Applied Biosystems
2720 Thermal Cycler Quick Reference Guide Rev B
Quick Reference Guide
4 Pages

Preview
Page 1
Applied Biosystems 2720 Thermal Cycler Quick Reference Card
Run IMPORTANT
Before operating this instrument, be sure to read safety information as instructed on the back of this card.
Main Menu 08:00 AM 09/25/00 25.0°C GeneAmp® PCR System Applied Biosystems Thermal2700 Cycler Version User: adrian Create Edit Util
Run F1
F2
F3
User
F4
F5
Stored Methods Methods appl01 exp000 exp001 exp002 Start View F1
F2
User adrian adrian adrian adrian User
Size 11 10 12 13
LastUsed 03/04/00 02/22/00 02/10/00 02/02/00
Sort
Cancel
F3
F4
F5
Go to Select User Name
Select Method Options Select Method Options Reaction Volume: 50 µL
Enter reaction volume here
View Method 2 Hld
Enter a value from 5 to 100 µL Start F1
F2
F3
Cancel
F4
55.0 2:00
F5
3 Tmp 25 Cycles 94.0 94.0 10:00 0:30
Start F1
55.0 0:30
2 Holds
72.0 72.0 0:30 5:00 4.0
Method: exp 001
Return
F3
F5
F2
F4
Run Time Sample 56.7 55.0 2:00
Cycle 1 of 25
94.0 94.0 10:00 0:30
HOT
72.0 0:30
55.0 0:30
72.0 5:00 4.0
Pause
Sorting Criteria
Info
F1
F2
F3
F4
F5
Sort Methods By: Method name Date last used Date stored Method size Accept
Cancel
F1
Pause Sample 56.7 55.0 2:00
Resume F1
94.0 0:30
55.0 0:30
HOT
72.0 0:30
72.0 5:00 4.0
Paused. Will resume in mm:ss F2
F3
F3
Method Information
Cycle 1 of 25
94.0 10:00
F2
F4
F5
02:32 PM Information 55.2°C Method: General PCR User: lisa Run started at 01:32:30 PM, 03/01/00. Run will end at 06:35:30 PM, 03/01/00. Reaction vol: 50 µL Return F1
F2
F3
F4
F5
F4
F5
Create/Edit Main Menu 08:00 AM 09/25/00 GeneAmp® PCR Thermal System Cycler 2700 Applied Biosystems
25.0°C
Stored Methods
Version User: adrian Create Edit Util
Run F1
F2
F3
User
F4
F5
Methods appl01 exp000 exp001 exp002 Edit View
User adrian adrian adrian adrian User
Size 11 10 12 13
LastUsed 03/04/00 02/22/00 02/10/00 02/02/00
Sort
Cancel
F1
F3
F4
F5
F2
Create/Edit You can highlight parameters and type in new values here. The More key is present only when you highlight a temperature or time.
1 Hld 3 Tmp 94.0 5:00
94.0 0:30
25 Cycles 2 Holds 72.0 0:30
55.0 0:30
72.0 7:00
4.0
Start
Store
More
Cancel
F1
F2
F3
F4
F5
The Modify key is present only when you highlight a PCR temperature or time. The Insert and Delete keys are present only when you highlight any temperature or time.
Store Method on Instrument
Modify
Store Method on Instrument
1 Hld 3 Tmp
User: april Method:exp002
94.0 5:00
94.0 0:30
F2
F3
F4
F5
72.0 0:30
55.0 0:30
Free Mem: 122 methods 946 segments Accept User Method Cancel F1
25 Cycles 2 Holds
Modify
Insert
Delete
More
F1
F2
F3
F4
abcdefghi jklmnopqrj Method Name exp001 stuvwxyz .,-+/():= Use ENTER key to select a character. Backsp F2
F5
Deletes highlighted segment
Method Name
F1
4.0
Go to Create/Edit
Go to Select User Name
Accept
72.0 7:00
Insert 2 Pre-PCR 3 Tmp 25 Cycles 55.0 2:00
94.0 94.0 10:00 0:30
*
55.0 0:30
72.0 0:30
2 Holds 72.0 5:00 4.0
Cancel
F3
F4
F5
Hold
Cycle
Pause
F1
F2
F3
Cancel F4
F5
Select Modification Select Modification AutoX - Increments or decrements time or temperature at the completion of each cycle AutoX F1
Inserts a hold or cycle to the left of the highlighted time or temperature
Cancel F2
F3
F4
2 Holds
+0.0 * +1.0 +0:00 -0:30 *
+
-
F1
F2
F3
Start first pause at cycle 1 of 25. Pause every 25 cycles for 00:30. Accept
2 Pre-PCR 3 Tmp 25 Cycles
Accept
Define Programmed Pause
F5
AutoX +0.0 +0:00
Define Programmed Pause
Cancel F4
F5
F1
Cancel F2
F3
F4
F5
Inserts a pause. Then you can edit the pause or go to Create/Edit
User Main Menu 08:00 AM 09/25/00 GeneAmp® PCR Thermal System Cycler 2700 Applied Biosystems Version User: adrian Create Edit Util
Run F1
F2
F3
F4
25.0°C
User F5
Select User Name Select User Name dave doug faye
<<ab>> adrian anna
jenife mamood mike
murray nayana ngui
Accept
New
Edit
Delete
Cancel
F1
F2
F3
F4
F5
Go to Main Menu
It's gone unless you have a method
User Name Highlight each letter then press Enter to spell the name.
abcdefghi jklmnopqrj stuvwxyz User Name .,-+/():= Use ENTER key to select a character. Accept Backsp Cancel F1
F2
F3
F4
Do you have a PIN number?
Yes
F5
No
Security Code
Security Check
User Name: hank
PIN number: None Protection: Unlocked Press PIN # to create a #. Then you set protection to Locked to prevent methods from being overwritten or deleted. Accept
Name
PIN#
F1
F2
F3
Security Check To perform this action, you must enter your PIN number. Your PIN #: Accept Cancel
Cancel F4
F5
F1
Go to Select User Name
F2
F3
F4
F5
New PIN Number Create a PIN Number
Go to User Name
Your PIN number protects the access to your user name and protection level Enter a PIN number. New PIN #: XXXX Accept
Cancel
F1
F2
F3
F4
F5
Protection Status
PIN Confirmation Confirm PIN Number Your PIN number protects the access to your user name and protection level Enter a PIN number again. PIN #: XXXX Press Accept to confirm your PIN #. Accept Cancel F1
F2
F3
F4
F5
Username: hank
PIN number: XXXX Protection: Unlocked Press PIN # to create a #. Then you set protection to Locked to prevent methods from being overwritten or deleted. Accept Name PIN# Lock Cancel F1
F2
F3
Go to Select User Name
F4
F5
Toggles Locked/ Unlocked
Stop or End of Run Stop pressed
Confirm Stop Sample 50.1
Confirm Stop
HOT
Press STOP to abort. Press Resume to continue. Resume F1
F2
F3
F4
F5
Go to Run Time
Stop pressed again
At end of run
End of Run 11:30 AM 25.1°C End of Run Method: General PCR Run completed at 11:30:05 AM, 02/01/00. Length of run is 01:34:25. Hist F1
Exit F2
F3
F4
F5
History File Has method been stored?
History of method exp002 User: adrian Reaction volume: 50 µL Run started at 02:30:45 PM, 02/01/00. Run aborted at 02:50:42 PM, 02/01/00. Length of run 00:19:57 No exceptions PageDn Print Return F1
F2
F3
F4
Yes
Go to Main Menu
No
F5
Method Not Stored Method Not Stored Press Store to save the method. Press Exit to delete the method. Store F1
F2
Exit F3
F4
F5
Go to Store Method on Instrument
Notice to Purchaser: Please refer to the Applied Biosystems 2720 Thermal Cycler User’s Manual for limited license or disclaimer statements. IMPORTANT Before operating this instrument, be sure to read the safety information in the Safety and EMC Compliance Information section of the Applied Biosystems 2720 Thermal Cycler User Guide. The user guide is provided in PDF on the CD that is shipped with the instrument. If you are unable to access the CD, you can view the user guide from the Applied Biosystems Web site http://www.appliedbiosystems.com/2720 From this Web site you can also request fax or e-mail delivery. In the United States and Canada you may call 1-800-762-4001. In other countries, contact your local sales office.
P/N 4359451 Rev. B