Applied Biosystems
3500 and 3500xL User Guide Rev C June 2010
User Guide
369 Pages

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Applied Biosystems 3500/3500xL Genetic Analyzer User Guide
User Guide For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures. © Copyright 2009, 2010 Life Technologies Corporation. All rights reserved. Information in this document is subject to change without notice. APPLIED BIOSYSTEMS DISCLAIMS ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. TO THE FULLEST EXTENT ALLOWED BY LAW, IN NO EVENT SHALL APPLIED BIOSYSTEMS BE LIABLE, WHETHER IN CONTRACT, TORT, WARRANTY, OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING BUT NOT LIMITED TO THE USE THEREOF, WHETHER OR NOT FORESEEABLE AND WHETHER OR NOT APPLIED BIOSYSTEMS IS ADVISED OF THE POSSIBILITY OF SUCH DAMAGES. NOTICE TO PURCHASER: The purchase price of this Instrument includes a grant of a limited, non-transferable license under U.S. patents and method claims of its foreign counterparts, and element clams of its foreign counterparts, to use this particular instrument for electrophoresis methods employing fluorescence as a means of detection. No other licenses or rights are hereby conveyed either expressly, by implication, or estoppel including, but not limited to, any claims to a composition. This instrument incorporates technology subject to one or more patents licensed from Hitachi, Ltd. as well as patents and patented technology owned by or under control of Applied Biosystems. This instrument is Authorized for use in DNA sequencing and fragments analysis only. This Authorization is included in the purchase price of the instrument and corresponds to the up-front fee component of a license under process claims of U.S. patents and under all process claims for DNA sequence and fragment analysis of U.S. patents now or hereafter owned or licensable by Applied Biosystems for which an Authorization is required, and under corresponding process claims in foreign counterparts of the foregoing for which an Authorization is required. The running royalty component of licenses may be purchased from Applied Biosystems or obtained by using Authorized reagents purchased from Authorized suppliers in accordance with the label rights accompanying such reagents. Purchase of this instrument does not itself convey to the purchaser a complete license or right to perform the above processes. This instrument is also licensed under U.S. patents and apparatus and system claims in foreign counterparts thereof. No rights are granted expressly, by implication, or by estoppel under composition claims or under other process or system claims owned licensable by Applied Biosystems. For more information regarding licenses, please contact the Director of Outlicensing at Applied Biosystems, 850 Lincoln Centre Drive, Foster City. California 94404, USA. TRADEMARKS: Trademarks of Life Technologies Corporation and its affiliated companies: Applied Biosystems®, AB (Design)®, AmpFlSTR®, BigDye®, BigDye Xterminator™, COfiler®, GeneMapper®, GeneScan™, Hi-Di™, Identifiler®, KB™, LIZ®, MicroSeq®, Minifiler™, POP-4™, POP-6™, POP-7™, Profiler®, Profiler Plus®, SEfiler Plus™, SeqScape®, SGM Plus®, SNaPshot®, Variant Reporter™, Yfiler® AFLP is a registered trademark of Keygene N.V. MLPA is a registered trademark of MRC-Holland. All other trademarks are the sole property of their respective owners.
Part Number 4401661 Rev. C 06/2010
Contents Preface... xiii Safety information... xiii About the product... xv Purpose of this guide... xv Audience... xv Assumptions... xv How to use this guide... xvi How to obtain support... xvii
Chapter 1
Instrument and Software Description... 1 System description... 1 Instrument description... 2 Instrument interior components... 3 Instrument parts and functions... 4 Theory of operation... 5 Preparing samples... 5 Preparing the instrument... 5 During a run... 5 Results... 6 Normalization... 7 Overview of the normalization feature... 7 When to use the normalization feature... 7 Materials for routine operation... 8 External barcode scanner... 8 Uninterruptible Power Supply (UPS)... 8 Instrument reagents and consumables... 9 Anode buffer container (ABC)... 9 Cathode buffer container (CBC)... 9 Polymers... 10 Conditioning reagent... 11 Hi-Di™ Formamide... 12 Capillary arrays... 12 Overview of the 3500 Series Data Collection Software... 14 About the software... 14 Parts of the software... 15
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Use the software without an instrument... 19
Chapter 2
Start the System... 21 Workflow... 21 Start the instrument... 22 Start the computer... 24 Log on to Windows... 24 Launch the application... 24 Log In... 26 Check system status in the Dashboard... 26 Dashboard, a quick glance... 26 Check maintenance notifications... 28 Check consumable status... 29 Check buffer fill levels... 31 Replenish consumables... 31 Set preferences... 32 Overview... 32 System preferences... 32 User preferences... 33 Set general preferences... 33 Set sequencing preferences... 36
Chapter 3
Set Up and Run... 41 Workflow... 41 Prepare the instrument... 42 Create a plate... 43 Assign plate contents... 46 Name samples and assign sample types in the plate view... 48 Assign assay, file name convention, and results group in the Plate View... 49 Print the plate layout... 50 Prepare and load sample plates... 51 Prepare sample plates... 52 Prepare the plate assembly... 53 Load the plate in the instrument... 53 Check instrument status... 53 Link the plate... 54 Quick Start a run... 55 Load plates for run and create the injection list... 56 Review and modify the injection list in Preview Run... 59 Start the run... 61 Monitor the run... 61 Check sequence or sample quality and specify re-injections... 62
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Check sequence or sample quality... 62 Specify re-injections... 65 Review completed injections in Review Results... 69 Start and stop a run... 69 More features in Assign Plate Contents... 70 Use the Plate View... 70 Use the Table View... 71 Sort and customize tables... 72 Add assays, file name conventions, and results groups to a plate... 73 Create a plate for importing... 73 Edit a plate... 74 Import and export a plate
... 75
Create a plate template... 75 Specify the default plate type for the Open Plate dialog box... 76 Save electronic version of reports... 76 More features in Load Plate for Run... 76 Link a plate from the recent plates or recent runs tab... 76 More features in Monitor Run... 77 Review the Instrument Run views... 77
Chapter 4
Review Results... 79 Review Sequencing Results... 80 Access the View Sequencing Results screen... 80 Review sequence quality... 81 Review traces... 82 Understand Quality Values (QVs)... 84 Specify re-injections... 85 View, print, and save (.pdf) trace quality reports... 85 Export sequencing results... 87 Review Fragment/HID Analysis results... 88 Access the View Fragment/HID Results screen... 88 Review sample quality... 89 Review normalized data... 90 Review plots... 91 Review sizing... 94 Specify re-injections... 95 View, print, and save (.pdf) sample quality reports... 95 Export sizing results... 96 More features in Review Results... 97 Use Rename... 97 Sort... 97 Modify sequence, fragment analysis, or HID data... 98
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Chapter 5
Calibrate and Check Performance... 99 Section 1 Calibration... 99 Spatial calibration... 99 When to perform a spatial calibration... 99 Perform a spatial calibration... 99 Evaluate the spatial calibration profile... 101 Example spatial profiles... 101 Export spatial calibration results... 101 View and print a spatial calibration report... 102 Save historical calibration reports (.pdf) for record keeping... 102 Spectral calibration... 103 When to perform a spectral calibration... 103 Prepare for the spectral calibration... 104 Perform a spectral calibration... 106 Evaluate the spectral calibration data... 110 What you see during a spectral calibration... 112 Example spectral calibration data... 114 Export spectral calibration results... 115 View and print a spectral calibration report... 116 Save historical calibration reports (.pdf) for record keeping... 116 View the spectral calibration history... 117
Section 2 Performance check... 119 Run the sequencing install standard performance check... 119 Prepare for the sequencing install standard performance check... 119 Run the sequencing install standard performance check... 122 What you see during a run... 124 How the software determines passing and failing capillaries for the spectral calibration 125 How the software determines passing and failing capillaries for the sequencing performance check... 125 Evaluate sequencing install standard data... 126 Example sequencing install standard results... 127 View previously run sequencing install standards... 127 View and print a sequencing install standard report... 127 Save historical performance check reports (.pdf) for record keeping... 128 Run the fragment analysis or HID Install standard performance check... 129 When to perform... 129 Prepare for the fragment or HID install standard performance check... 129 Run the fragment analysis or HID install standard performance check... 132 What you see during a run... 134 How the software determines passing and failing capillaries for the fragment/HID performance check... 135 vi
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Evaluate fragment/HID install standard data... 135 Example fragment install standard results... 137 Example HID install standard results... 137 View previously run install standards... 137 View and print a fragment or HID install standard detail report... 137 Save historical performance check reports (.pdf) for record keeping... 138
Chapter 6
Manage Library Resources... 139 Overview of libraries... 139 General library procedures... 140 Access libraries... 140 Create a new entry from a factory-provided, template, or locked entry... 140 Delete a library entry... 141 Edit a library entry... 141 Import and export a library entry... 141 View audit and e-signature histories for library entries... 142 Sort, filter, and search library entries... 142 Customize a library table... 143 Plates library... 143 Plate overview... 143 Create a new plate... 144 Assays library... 147 Assay overview... 147 Create a new assay... 148 File name conventions library... 151 File name convention overview... 151 Create a new file name convention... 151 Result group library... 155 Results group overview... 155 Create a new results group... 156 Results group example 1: store files by plate name... 160 Results group example 2: store one allelic ladder per run folder (8-capillary instruments)... 161 Results group example 3: store re-injections in separate folders... 162 Instrument protocol library... 165 Instrument protocol overview... 165 Create a new instrument protocol... 165 Dye sets library... 168 Dye set overview... 168 Create a new dye set... 168 Size standards library... 171 Size standard overview... 171 Normalization size standards provided... 171
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Create a new size standard... 172 Modify a factory-provided normalization size standard... 173 Basecalling protocols library (primary analysis – sequencing)... 174 Basecalling protocol overview... 174 Create a new basecalling protocol... 174 Sizecalling protocols library (primary analysis – fragment)... 179 Sizecalling protocol overview... 179 Create a new sizecalling protocol... 179 QC protocols library (primary analysis – HID)... 184 QC protocol overview... 184 Create a new QC protocol... 184 Sequencing analysis protocols library (secondary analysis)... 189 Sequencing analysis protocol overview... 189 Create a new sequencing analysis protocol... 189 MicroSeq® ID protocols library (secondary analysis)... 191 MicroSeq® ID analysis protocol overview... 191 Create a new MicroSeq® ID analysis protocol... 191 Fragment analysis protocols library (secondary analysis)... 193 Fragment analysis protocol overview... 193 Create a new fragment analysis protocol... 193 HID analysis protocols library (secondary analysis)... 195 HID analysis protocol overview... 195 Create a new HID analysis protocol... 195
Chapter 7
Use Security, Audit, and E-Sig Functions (SAE Module) . . . 197 Section 1 Administrators... 197 Administrators overview of system security, auditing, and electronic signature... 197 Configure the security system... 198 Access the Security screen and enable or disable security... 198 Set account setup and security policies... 199 Set up messaging notifications... 200 Manage user accounts... 201 Create or edit a user account... 201 Determine the name of the logged-in user... 203 Create or edit a user role... 203 View and print a user report... 205 Manage auditing... 207 Access the Audit screen and enable or disable auditing... 207 Select objects to audit... 208 Create audit reason settings... 208 Generate audit reports... 209 Display audit histories... 209
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Review the object audit history... 210 Review the system configuration history... 211 Review the action log... 212 View and print audit reports... 213 Archive, purge, and restore audit records... 214 Export audit records... 215 Manage electronic signature... 216 Access the E-Signature Settings screen and enable or disable e-sig... 216 Select the actions that allow signature... 217 Generate e-signature reports... 221 Display e-signature records... 221 View and print e-signature reports... 222 Export e-sig records... 223 Export and import user accounts, security, audit, and electronic signature settings . . 224
Section 2 Users... 225 Users overview of System Security, Audit Trail and E-Signature... 225 Security... 225 Audit... 227 Electronic signature... 227
Chapter 8
Maintain the Instrument... 229 Maintenance schedule... 229 Review maintenance notifications... 229 Daily instrument maintenance tasks... 230 Weekly instrument maintenance tasks
... 231
Monthly instrument maintenance tasks... 231 Quarterly maintenance tasks... 231 Annual planned maintenance tasks... 232 As-needed instrument maintenance tasks
... 232
Use the maintenance calendar... 232 View the calendar... 232 Default calendar entries... 233 Create calendar entries... 233 Review the Maintenance Notifications Log... 234 Instrument operational procedures... 236 Check consumables on the Dashboard... 236 Change the anode buffer container (ABC)... 237 Change the cathode buffer container (CBC)... 238 Check stored capillary arrays... 240 Flush the water trap (pump trap)... 241 Routine instrument cleaning... 242 Move and level the instrument... 243 Applied Biosystems 3500/3500xL Genetic Analyzer User Guide
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Use the Maintenance Wizards to perform operations... 244 About Maintenance Wizards... 244 Replenish polymer... 245 Change polymer type... 247 Partially used polymer... 249 Wash the pump chamber and channels... 249 Use the conditioning reagent... 250 Fill capillary array with fresh polymer... 251 Remove bubbles from the polymer pump... 251 To change the capillary array... 252 To shutdown the instrument... 253 Computer maintenance... 254 Uninstall the software... 254 Archive, purge, and restore data... 254 Monitor disk space... 256 Review the Maintenance Notifications Log... 257 Service Log... 258
Appendix A Application Reagents and Run Modules... 259 Sequencing analysis reagents... 259 Fragment and HID analysis reagents... 260 Sequencing analysis dye sets for all applications... 261 Fragment analysis dye sets for all applications... 261 HID analysis dye sets... 262 Run modules... 263 Capillary array and polymer (sequencing analysis run modules)... 263 Capillary array and polymer (fragment and HID analysis run modules)... 264
Appendix B Secondary Analysis: Sequencing... 267 Perform secondary analysis on sequencing experiments... 267 Auto-analyze projects in the sequencing analysis software... 267 Set up an auto-analysis project in SeqScape®... 268 Set up a SeqScape plate in the 3500 Series Data Collection Software... 270 Auto-analysis with MicroSeq® ID... 277
Appendix C Secondary Analysis: Fragment... 279 Perform secondary analysis on fragment experiments... 279 Auto-analyze projects in the fragment analysis software... 279 Set up an auto-analysis project in GeneMapper®... 280 Set up a GeneMapper plate in the 3500 Series Data Collection Software... 284 Auto-Analysis with GeneMapper® ID-X... 289 x
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Appendix D Remote Auto-Analysis Setup... 291 Remote auto-analysis configuration... 291 Remote auto-analysis installation... 291 Create a shared folder... 294 Set up the 3500 Series Data Collection Software v1.0... 297
Appendix E
Troubleshoot... 299 Instrument troubleshooting... 299 Spatial calibration troubleshooting... 300 Spectral calibration troubleshooting... 301 Sequencing install standard troubleshooting... 302 Fragment/HID install standard troubleshooting... 303 Anode buffer container troubleshooting... 303 Cathode buffer container troubleshooting... 304 RFID troubleshooting... 304 Link a plate troubleshooting... 304 How to search and use the log files... 305 View instrument sensor details... 305 Data/electropherogram troubleshooting
... 306
Dashboard troubleshooting... 309 Load plate troubleshooting... 309 Monitor run troubleshooting... 311 Review results troubleshooting... 311 Review error message details... 312 Audit troubleshooting... 312 Electronic signature troubleshooting... 312 Manual commands troubleshooting... 313 Miscellaneous... 313 Reset the instrument... 314
Appendix F
Safety... 315 Instrumentation safety... 315 Symbols on instruments... 315 Safety labels on instruments... 317 General instrument safety... 317 Physical hazard safety... 318 Electrical safety... 319 Laser safety... 320 Bar code scanner laser safety... 321 Workstation safety... 321 Safety and electromagnetic compatibility (EMC) standards... 322
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Chemical safety... 328 General chemical safety... 328 MSDSs... 329 Chemical waste safety... 330 Biological hazard safety... 331 Safety alerts... 332 Chemical alerts... 332 Instrumentation alerts... 334
Documentation... 335 Related documentation... 335 Obtaining information from the Help system... 336 Send us your comments... 336
Index... 337
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Applied Biosystems 3500/3500xL Genetic Analyzer User Guide
Preface Safety information Note: For general safety information, see this Preface and Appendix F, “Safety” on page 315. When a hazard symbol and hazard type appear by a chemical name or instrument hazard, see Appendix F, “Safety” on page 315 for the complete alert on the chemical or instrument.
Safety alert words
Four safety alert words appear in Applied Biosystems user documentation at points in the document where you need to be aware of relevant hazards. Each alert word-IMPORTANT, CAUTION, WARNING, DANGER-implies a particular level of observation or action, as defined below: IMPORTANT! – Indicates information that is necessary for proper instrument
operation, accurate chemistry kit use, or safe use of a chemical. CAUTION! – Indicates a potentially hazardous situation that, if not avoided, may result in minor or moderate injury. It may also be used to alert against unsafe practices. WARNING! – Indicates a potentially hazardous situation that, if not avoided, could result in death or serious injury. DANGER! – Indicates an imminently hazardous situation that, if not avoided, will result in death or serious injury. This signal word is to be limited to the most extreme situations.
Except for IMPORTANTs, each safety alert word in an Applied Biosystems document appears with an open triangle figure that contains a hazard symbol. These hazard symbols are identical to the hazard symbols that are affixed to Applied Biosystems instruments (see “Safety symbols” on page 315).
MSDSs
The MSDSs (Material Safety Data Sheets) for any chemicals supplied by Applied Biosystems or Ambion are available to you free 24 hours a day. For instructions on obtaining MSDSs, see “MSDSs” on page 329. IMPORTANT! For the MSDSs of chemicals not distributed by Applied Biosystems
or Ambion contact the chemical manufacturer.
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Preface
Safety labels on instruments Hazard symbol
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The following CAUTION, WARNING, and DANGER statements may be displayed on Applied Biosystems instruments in combination with the safety symbols described in the preceding section. English
Français
CAUTION! Hazardous chemicals. Read the Material Safety Data Sheets (MSDSs) before handling.
ATTENTION! Produits chimiques dangereux. Lire les fiches techniques de sûreté de matériels avant toute manipulation de produits.
CAUTION! Hazardous waste. Refer to MSDS(s) and local regulations for handling and disposal.
ATTENTION! Déchets dangereux. Lire les fiches techniques de sûreté de matériels et la régulation locale associées à la manipulation et l’élimination des déchets.
CAUTION! Potential slipping hazard.
ATTENTION! Risque potentiel d'avoir un sol glissant.
CAUTION! Hot surface.
ATTENTION! Surface brûlante.
DANGER! High voltage.
DANGER! Haute tension.
WARNING! To reduce the chance of electrical shock, do not remove covers that require tool access. No user-serviceable parts are inside. Refer servicing to Applied Biosystems qualified service personnel.
AVERTISSEMENT! Pour éviter les risques d’électrocution, ne pas retirer les capots dont l’ouverture nécessite l’utilisation d’outils. L’instrument ne contient aucune pièce réparable par l’utilisateur. Toute intervention doit être effectuée par le personnel de service qualifié venant de chez Applied Biosystems.
CAUTION! Class 2(II) visible and/or invisible radiation present. Do not stare directly into the beam or view directly with optical instruments.
ATTENTION! Rayonnement visible ou invisible d’un faisceau. Ne pas regarder le faisceau directement ou au travers d’un instrument optique.
DANGER! Class 3B (III) visible and/or invisible radiation present. Avoid exposure to beam.
DANGER! Rayonnement visible ou invisible d’un faisceau de Classe 3B (III) en cas d’ouverture. Evitez toute exposition au faisceau.
CAUTION! Sharp object.
AVERTISSEMENT! Objet Sharp.
Applied Biosystems 3500/3500xL Genetic Analyzer User Guide
About the product
About the product The Applied Biosystems 3500/3500xL Genetic Analyzers is an automated 8 and/or 24 capillary instrument designed for a wide range of sequencing and fragment analysis applications. IMPORTANT! For Research Use Only. Not for use in diagnostic procedures.
Purpose of this guide The Applied Biosystems 3500/3500xL Genetic Analyzer User Guide provides stepby-step instructions for preparing and analyzing a sample. It is designed to help you learn how to use the instrument. CAUTION! The protection provided by the equipment may be impaired if the instrument is operated outside the environment and use specifications, the user provides inadequate maintenance, or the equipment is used in a manner not specified by the manufacturer (Applied Biosystems).
Audience This user guide is written for principle investigators and laboratory staff who are planning to operate and maintain the Applied Biosystems 3500/3500xL Genetic Analyzers.
Assumptions The Applied Biosystems 3500/3500xL Genetic Analyzer User Guide assumes that your 3500 or 3500xL analyzer has been installed by an Applied Biosystems service representative. This guide also assumes you have the following background: • Familiarity with Microsoft® Windows Vista® operating system. • Knowledge of general techniques for handling DNA samples and preparing them for electrophoresis. • A general understanding of hard drives, data storage, file transfers, and copying and pasting.
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Preface
How to use this guide Text conventions
This guide uses the following conventions: • Bold text indicates user action. For example: Type 0, then press Enter for each of the remaining fields. • Italic text indicates new or important words and is also used for emphasis. For example: Before changing reagents, always determine what chemicals have been used in the instrument. • A right arrow symbol () separates successive commands you select from a drop-down or shortcut menu. For example: Select FileOpenSpot Set. Right-click the sample row, then select View Filter View All Runs.
User attention words
Two user attention words appear in Applied Biosystems user documentation. Each word implies a particular level of observation or action as described below: Note: Provides information that may be of interest or help but is not critical to the
use of the product. IMPORTANT! Provides information that is necessary for proper instrument
operation, accurate chemistry kit use, or safe use of a chemical.
Table of Acronyms
The following table explains the acronyms used in the Applied Biosystems 3500/3500xL Genetic Analyzer User Guide. Acronym
Definition
ABC
Anode Buffer Container
BDT
BigDye® Terminator Kit
BDX
BigDye® Xterminator™
Cap
Capillaries
CBC
Cathode Buffer Container
CV/Fitting
Check Valve pouch attachment fitting
EPT
ElectroPhoresis Telemetry
FR
Factory Repeating
MicroSeq® kit
Microbial Sequencing
(or other product name) NIC
Network Interface Card
NT
Nucleoid Type Nucleotide Base Color (A, G, C, T)
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Pe
Probability of error
QV
Quality Value Applied Biosystems 3500/3500xL Genetic Analyzer User Guide
How to obtain support
Acronym
Definition
GM
GeneMapper® Software
GMIDx
GeneMapper® IDx
POP™
Polymer (Brand name of the polymer)
PPS
Power Protection System
SAE
Security, Administration, Electronic signature
How to obtain support For the latest services and support information for all locations, go to: www.appliedbiosystems.com At the Applied Biosystems web site, you can: • Access worldwide telephone and fax numbers to contact Applied Biosystems Technical Support and Sales facilities. • Search through frequently asked questions (FAQs). • Submit a question directly to Technical Support. • Order Applied Biosystems user documents, MSDSs, certificates of analysis, and other related documents. • Download PDF documents. • Obtain information about customer training. • Download software updates and patches.
How to obtain more information
For detailed information on preparing for installation, refer to the Applied Biosystems 3500 Series Genetic Analyzer Site Preparation Guide (4401689). Note: The purpose of the Site Prep Guide is to help you prepare your site for installation of the 3500 or 3500xL analyzer. For specific details about your system, please refer to this user guide.
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Applied Biosystems 3500/3500xL Genetic Analyzer User Guide
Instrument and Software Description
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System description The 3500 or 3500xL analyzer is shipped with the following system components: • Capillary Electrophoresis instrument. • 3500 (8-capillary) or 3500xL (24-capillary) array and POP™ polymer • DNA sequencing, or fragment analysis, reagents, and other consumables for system qualification. • Dell® computer workstation with flat-screen monitor. • Integrated software for instrument control, data collection, quality control, and basecalling or sizing of samples.
Applied Biosystems 3500/3500xL Genetic Analyzer User Guide
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Chapter 1 Instrument and Software Description
Instrument description The Applied Biosystems 3500/3500xL Genetic Analyzers are fluorescence based DNA analysis instrument using capillary electrophoresis technology with 8- or 24capillaries. For detailed dimensions of the instrument, refer to the Applied Biosystems 3500 Series Genetic Analyzer Site Preparation Guide (4401689). Note: The purpose of the Site Prep Guide is to help you prepare your site for installation of the 3500 or 3500xL analyzer. For specific details about your system, please refer to this user guide.
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Applied Biosystems 3500/3500xL Genetic Analyzer User Guide