Applied Biosystems

7300, 7500 and 7500 Fast Relative Quantification Getting Started Guide Rev B June 2010

Getting Started Guide

106 Pages

Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System  Introduction and Example RQ Experiment  Relative Quantification Getting Started Guide  Designing an RQ Experiment  Primer Extended on mRNA 5′  3′ Reverse Primer  5′ cDNA  Oligo d(T) or random hexamer  Synthesis of 1st cDNA strand 5′ cDNA  3′  Performing Reverse Transcription  STANDARD  Generating Data from RQ Plates – 7300 or Standard 7500 System  FAST  Generating Data from RQ Plates – 7500 Fast System  Analyzing Data in an RQ Study
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File Name: Applied Biosystems - 7300, 7500 and 7500 Fast Relative Quantification Getting Started Guide Rev B June 2010 - 2010-06.pdf

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