Applied Biosystems
ViiA 7 Site Preparation Guide Rev B Dec 2010
Site Preparation Guide
56 Pages

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Applied Biosystems ViiA™ 7 Real-Time PCR System Site Preparation Guide
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. Information in this document is subject to change without notice. APPLIED BIOSYSTEMS DISCLAIMS ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. TO THE FULLEST EXTENT ALLOWED BY LAW, IN NO EVENT SHALL APPLIED BIOSYSTEMS BE LIABLE, WHETHER IN CONTRACT, TORT, WARRANTY, OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING BUT NOT LIMITED TO THE USE THEREOF, WHETHER OR NOT FORESEEABLE AND WHETHER OR NOT APPLIED BIOSYSTEMS IS ADVISED OF THE POSSIBILITY OF SUCH DAMAGES. TRADEMARKS The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. Dell is a trademark of Dell Inc. Milli-Q is a trademark of Millipore Corporation. Twister is a trademark of Caliper Life Sciences Inc. © 2010 Life Technologies Corporation. All rights reserved.
Part Number 4445302 Rev. B 12/2010
Contents
About This Guide... 7 About this document... 7 Purpose of this guide... 7 Audience... 7 User attention words... 7 Safety conventions used in this document... 8 Safety alert words... 8 Definitions... 8 Examples... 8 Safety information... 10 Safety labels on instruments... 10 How to obtain support... 11 How to obtain more information... 12 Related documentation... 12 Send us your comments... 12
CHAPTER 1
Site Preparation Tasks... 13 Instrument overview... 14 Installation overview... 14 Site preparation schedule... 14 Site preparation process... 15 Assign personnel... 16 Laboratory safety representative... 16 Tasks and personnel... 16 Select the site... 18 Space requirements... 19 System components... 19 Setup requirements... 19 Layout requirements... 20 Dimensions and weights... 21 Required clearances... 21 Environmental requirements... 22 Altitude... 22 Temperature and humidity requirements... 22 Pollution... 22 Environmental conditions for storage and transportation... 22
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Contents
Instrument hot-air exhaust venting... 22 Waste collection requirements... 23 Collecting liquid... 23 Electrical requirements... 23 System electrical requirements... 23 Electrical protective devices... 24 Computer requirements... 25 Antivirus software requirements... 25 Network requirements... 25 Safety and materials... 26 Safety practices... 26 Required safety equipment... 26 Materials for installation... 27 Materials for routine operation... 27 Receiving and inspecting the system... 28 Shipped contents... 28 Shipping list... 28 Inspect shipping containers for damage... 28 Unpack and store the Installation Kit... 28 Inspecting shipping containers for damage... 29 Moving the crated instrument to the laboratory... 29 Moving schedule... 29 During installation... 30 Operator training... 30
CHAPTER 2
Checklists... 31 Overview... 32 Personnel checklist... 32 Space and layout checklist... 32 Environmental checklist... 33 Electrical checklist... 33 Computer checklist... 33 Safety checklist... 34 Materials checklist... 34 System receipt and inspection checklist... 35 Moving the crated instrument checklist... 35
APPENDIX A
Safety and EMC Compliance Information... 37 Symbols on instruments... 38 Electrical symbols on instruments... 38 Safety symbols... 38 Environmental symbols on instruments... 39
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Contents
Location of safety symbols... 40 General instrument safety... 41 Moving and lifting the instrument... 41 Moving and lifting stand-alone computers and monitors... 41 Operating the instrument... 41 Cleaning or decontaminating the instrument... 42 Chemical safety... 42 Chemical hazard warning... 42 About SDSs... 42 Obtain an SDS... 42 Chemical safety guidelines... 43 Chemical waste safety... 44 Chemical waste hazard... 44 Chemical waste safety guidelines... 44 Waste disposal... 45 Electrical safety... 45 Power... 45 Overvoltage rating... 45 Fuses... 46 Bar code scanner laser safety... 46 Physical hazard safety... 46 Ultraviolet light... 46 Moving parts... 46 Biological hazard safety... 47 General biohazard... 47 Workstation safety... 47 Safety and electromagnetic compatibility (EMC) standards... 48 U.S. and Canadian safety standards... 48 Canadian EMC standard... 48 European safety and EMC standards... 48 Australia and New Zealand EMC standards... 49
Index... 51
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Contents
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Applied Biosystems ViiA™ 7 Real-Time PCR System
About This Guide
About this document Purpose of this guide The Applied Biosystems ViiA™ 7 Real-Time PCR System Site Preparation Guide provides the information you need to fully prepare your site for the arrival and installation of the system. Complete preparation helps ensure a smooth installation process, as well as correct and safe instrument operation.
Audience This guide is intended for the personnel who will schedule, manage, and perform the tasks required to prepare your site for installation of the Applied Biosystems ViiA™ 7 Real-Time PCR System.
User attention words Two user attention words appear in Applied Biosystems user documentation. Each word implies a particular level of observation or action as described below: Note: – Provides information that may be of interest or help but is not critical to the use of the product. IMPORTANT! – Provides information that is necessary for proper instrument operation, accurate chemistry kit use, or safe use of a chemical. Examples of the user attention words appear below: Note: For additional documentation, see “How to obtain support” on page 11. IMPORTANT! To verify your client connection to the database, you need a valid user ID and password.
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About This Guide Safety conventions used in this document
Safety conventions used in this document Safety alert words Four safety alert words appear in Applied Biosystems user documentation at points in the document where you need to be aware of relevant hazards. Each alert word- IMPORTANT, CAUTION, WARNING, DANGER-implies a particular level of observation or action, as defined below.
Definitions IMPORTANT! – Indicates information that is necessary for proper instrument operation, accurate chemistry kit use, or safe use of a chemical.
CAUTION! – Indicates a potentially hazardous situation that, if not avoided, may result in minor or moderate injury. It may also be used to alert against unsafe practices.
WARNING! – Indicates a potentially hazardous situation that, if not avoided, could result in death or serious injury.
DANGER! – Indicates an imminently hazardous situation that, if not avoided, will result in death or serious injury. This signal word is to be limited to the most extreme situations. Except for IMPORTANTs, each safety alert word in an Applied Biosystems document appears with an open triangle figure that contains a hazard symbol. These hazard symbols are identical to the hazard symbols that are affixed to Applied Biosystems instruments (see “Safety symbols” on page 38).
Examples The following examples show the use of safety alert words: IMPORTANT! You must create a separate sample entry spreadsheet for each 384-well plate.
CAUTION! Do not install on the computer additional software other than antivirus software. Changes to the configured software could void the instrument warranty and cause the system to be nonoperational.
WARNING! Physical Injury Hazard. Using the instrument in a manner not specified by Applied Biosystems may result in personal injury or damage to the instrument
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Safety conventions used in this document
DANGER! ELECTRICAL HAZARD. Failure to ground the instrument properly can lead to an electrical shock. Ground the instrument according to the provided instructions.
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How to obtain support
How to obtain support For the latest services and support information for all locations, go to www.appliedbiosystems.com, then click the link for Support. At the Support page, you can: • Search through frequently asked questions (FAQs) • Submit a question directly to Technical Support • Order Applied Biosystems user documents, certificates of analysis, and other related documents • Order Safety Data Sheets (SDSs) for any chemicals supplied by Applied Biosystems or Ambion (available to you free 24 hours a day). • Download PDF documents • Obtain information about customer training • Download software updates and patches In addition, the Support page provides access to worldwide telephone and fax numbers to contact Applied Biosystems Technical Support and Sales facilities.
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About This Guide How to obtain more information
How to obtain more information Related documentation The following related documents are part of your shipment: • Applied Biosystems ViiA™ 7 Real-Time PCR System User Guide – The user guide provides step-by-step instructions for preparing and analyzing a sample. It is designed to help you learn how to use the system. • Applied Biosystems ViiA™ 7 Real-Time PCR System Quick Reference Card – The Quick Reference Card contains a flowchart on how to run the samples and system, a table of maintenance tasks, and a software reference guide. • Applied Biosystems ViiA™ 7 Real-Time PCR System Getting Started Guide – The Getting Started Guide is a tutorial for performing your experiments. Portable document format (PDF) versions of the User Guide and Quick Reference Card are also available on the Software CD. Note: To view the user documentation, you can use the Adobe® Acrobat® Reader® software available from www.adobe.com. Note: For additional documentation, see “How to obtain support” on page 11.
Send us your comments Applied Biosystems welcomes your comments and suggestions for improving its user documents. You can e-mail your comments to: [email protected] IMPORTANT! The e-mail address above is only for submitting comments and suggestions relating to documentation. To order documents, download PDF files, or for help with a technical question, go to www.appliedbiosystems.com, then click the link for Support. (See “How to obtain support”).
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CHAPTER 1
Site Preparation Tasks
1 ■
Instrument overview... 14
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Installation overview... 14
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Assign personnel... 16
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Select the site... 18
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Space requirements... 19
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Environmental requirements... 22
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Waste collection requirements... 23
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Electrical requirements... 23
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Computer requirements... 25
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Safety and materials... 26
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Materials for routine operation... 27
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Receiving and inspecting the system... 28
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Moving the crated instrument to the laboratory... 29
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During installation... 30
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Chapter 1 Site Preparation Tasks Instrument overview
Instrument overview The instrument uses fluorescent-based polymerase chain reaction (PCR) reagents to provide: • Quantitative detection of target nucleic acid sequences (targets) using real-time analysis. • Qualitative detection of targets using post-PCR (endpoint) analysis. • Qualitative analysis of the PCR product (achieved by melt-curve analysis that occurs post-PCR). The instrument is to be used only by technologists trained in laboratory techniques and procedures and in the use of the instrument. The customer is responsible for validation of any assays, and compliance with any regulatory requirements that pertain to their procedures and uses of the instrument.
Installation overview Before an Applied Biosystems service representative arrives to install the instrument and associated devices, prepare your site for the installation according to the instructions in this chapter. For checklists, see“Checklists” on page 31. IMPORTANT! If site preparation tasks are not complete when the Applied Biosystems service representative arrives, the scheduled installation may be postponed.
Site preparation schedule To minimize the time between the shipment arrival and installation:
1. Complete the site preparation tasks in this chapter. 2. Fill out the corresponding checklists. See “Checklists” on page 31. 3. Schedule installation before the shipment arrives. 4. Verify with an Applied Biosystems service representative who will contact you by telephone that: • All checklists are complete • The purchase order is complete • You have considered all components and options in preparing the site
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Chapter 1 Installation overview
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Site preparation process The general site preparation tasks and a suggested sequence for completing the tasks are summarized in Figure 1,“Site preparation tasks and their suggested sequence”, below. The sequence can vary, but always: • Review this guide first • Unpack and store the Installation Kit as soon as you receive it Figure 1 Site preparation tasks and their suggested sequence
Review this guide Stock the site
Assign personnel Receive and inspect the system Select the installation site
Meet space, utility, computer, and environment
Applied Biosystems ViiA™ 7 Real-Time PCR System
Unpack and store the Installation Kit
Move the system to the installation site
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Chapter 1 Site Preparation Tasks Assign personnel
Assign personnel Laboratory safety representative Applied Biosystems requests that a representative from your laboratory be in the vicinity and available to the Applied Biosystems service representative at all times while the service representative is at your facility. The laboratory safety representative should be familiar with laboratory safety procedures and know the location of all the safety equipment.
Tasks and personnel Table 1, Suggested personnel tasks, summarizes specific site-preparation tasks and suggests the personnel to accomplish the tasks. Use the table to help schedule and manage the site-preparation process. Table 1 Suggested personnel tasks Personnel Site preparation-installation coordinator
Tasks • Orders required materials. • Chooses the site. • Reviews checklists with applicable personnel, then verifies with the Applied Biosystems service representative that the site is properly prepared. • Schedules the installation and informs personnel of the installation date. • Ensures that the site is clear of unnecessary material on the installation day. • Is available to assist the service representative throughout installation.
Laboratory safety representative
• Ensures that the required safety practices and equipment are in place. • Is available to assist with unpacking and setup.
Laboratory personnel-primary users
• Ensures that all customer-provided materials for installation are present at the site. • Primary users (responsible for training other users) are available during the installation, so that they can be trained on the instrument. • Primary users have access to an active email account and networked computer in order to acquire software license during installation.
Facilities personnel-primary users
• Ensures that installation requirements are met for: – Space at the installation site – Building clearances – Temperature and humidity – Waste collection – Electrical supply – Computer – Safety and installation materials • At least two people are available to help the Applied Biosystems service representative move and position the system.
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Chapter 1 Assign personnel
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Personnel
Tasks
Network or IT specialist (if the system will be connected to a network)
• Ensures that two active, tested local area network (LAN) connections are in place before the scheduled installation date. • Ensures that network hardware is compatible with an RJ45-type connector. • Is available during installation to connect the system to the network.
CAUTION! Do not attempt to connect the system components to the network before the Applied Biosystems service representative arrives.
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Chapter 1 Site Preparation Tasks Select the site
Select the site When deciding where to install the instrument, refer to the following sections for site requirements: • “Space requirements” on page 19 • “Environmental requirements” on page 22 • “Waste collection requirements” on page 23 • “Electrical requirements” on page 23 • “Computer requirements” on page 25 • “Safety and materials” on page 26 IMPORTANT! The site cannot be designated BioSafety Level 3 (BSL-3) or BioSafety Level 4 (BSL-4). Applied Biosystems does not install, service, or repair Applied Biosystems instruments in areas designated BSL-3 or BSL-4.
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Chapter 1 Space requirements
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Space requirements System components The shipment shown in Figure 2 includes the: • Instrument • Dell® computer, keyboard and mouse • Dell® flat screen monitor
Setup requirements A typical setup is shown in Figure 2 and Figure 3. For details on the space requirements, see “Required clearances” on page 21. Figure 2 Setup requirements (not to scale) 15.24 cm (6 in.) 78.8 cm (31 in.)
63.5 cm (25 in.)
53.3 cm (21 in.) 162.56 cm (64 in.) 15.24 cm (6 in.)
64.5 cm (25.4 in.)
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Chapter 1 Site Preparation Tasks Space requirements
Figure 3 Setup requirements - showing optional robot (not to scale)
15.24 cm (6 in.) 81.28 cm (32 in.)
63.5 cm (25 in.)
53.3 cm (21 in.)
53.3 cm (21 in.)
213.36 cm (84 in.)
97.0 cm (38 in.)
Layout requirements Verify that the installation location: • Is not adjacent to vibration sources, such as a centrifuge, pump, or compressor. Excessive vibration will affect instrument performance. • Is not adjacent to electrically noisy devices, such as a refrigeration unit. • Allows the computer to be within 2 meters (6 feet) of the instrument. • Allows positioning of the monitor, keyboard, and accessories for proper ergonomics during use. • Is a stable and level surface. The instrument and robot must be level to function properly.
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Chapter 1 Space requirements
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Dimensions and weights The table below indicates dimensions and weights of the instrument and associated devices. Ensure that the installation site (floor space and/or bench space) can accommodate the dimensions and support the weights. Table 2 Dimensions and weights of instrument and associated devices Component
Width
Depth
Height
Weight
Instrument
53.3 cm (21.0 in)
63.5 cm (_25.0 in)
64.5 cm (25.4 in)
60.7 kg (134 lbs)
Computer (desktop)
18.7 cm (7.36 in)
44.5 cm (17.52 in)
41 cm (16.1 in)
10.9 kg (24 lbs)
Computer (laptop) (optional)
35.8 cm (14.1 in)
25.7 cm (10.1 in)
35.8 cm (14.1 in)
2.6 kg (5.7 lbs)
• Monitor
44.7 cm (17.5 in)
19.3 cm (7.6 in)
36.6 cm (14.4 in)
6.9 kg (15.2 lbs)
• Keyboard
44.7 cm (17.5 in)
15.25 cm (6 in)
5 cm (2 in)
.09 kg (0.2 lbs)
Twister II Robot (optional)
53.3 cm [21 in]
77 cm [28 in]
97 cm [38 in]
49.9 kg [110 lbs]
Computer accessories
Required clearances During instrument setup and maintenance, it is necessary to access the back of the instrument. If the back of the instrument faces a wall, it will be necessary to have enough space to rotate the instrument on the bench for access. Required clearances are: • Clearance – At least 15.2 cm (6 in) of clearance for ventilation, service access, and cable routing at the back of the instrument. The total width of the required space with the instrument and a desktop computer will be 162.5 cm (64 in) and the total depth will be 81.3 cm (32 in). If a robot is included, the total width will be 213 cm (84 in). • Vertical clearance – Including instrument height, total vertical space required will be 80 cm (31.4 in). If a robot is included, the total vertical space required will be 112 cm (44 in).
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