Models CB series Operating Manual Sept 2013

Operating Manual

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Operating Manual APT.line™ CB (E6) CO2 - Incubators CO2 – Incubators with O2 control with sterilizable NDIR sensor system for CO2 and humidity and microprocessor controller T4.12 Model

Voltage

Equipment

CB 60

230 V

9040-0088, 9140-0088

CB 60-UL

100-120V

9040-0089, 9140-0089

CB 60

230 V

with O2 control

9040-0090, 9140-0090

CB 60-UL

100-120V

with O2 control

9040-0091, 9140-0091

CB 160 (E6)

230 V

9040-0092, 9140-0092

CB 160-UL

100-120V

9040-0093, 9140-0093

CB 160 (E6)

230 V

with O2 control

9040-0094, 9140-0094

CB 160-UL

100-120V

with O2 control

9040-0095, 9140-0095

CB 160

230 V

with divided glass door

9040-0100, 9140-0100

CB 160-UL

100-120V

with divided glass door

9040-0101, 9140-0101

CB 160

230 V

with O2 control

with divided glass door

9040-0102, 9140-0102

CB 160-UL

100-120V

with O2 control

with divided glass door

9040-0103, 9140-0103

CB 220

230 V

9040-0096, 9140-0096

CB 220-UL

100-120V

9040-0097, 9140-0097

CB 220

230 V

with O2 control

9040-0098, 9140-0098

CB 220-UL

100-120V

with O2 control

9040-0099, 9140-0099

BINDER GmbH Address Tel. Fax Internet E-mail Service Hotline Service Fax Service E-Mail Service Hotline USA Service Hotline Asia Pacific Service Hotline Russia and CIS Issue 09/2013

Art. No.

Post office box 102 D-78502 Tuttlingen +49 7462 2005 0 +49 7462 2005 100 http://www.binder-world.com [email protected] +49 7462 2005 555 +49 7462 2005 93 555 [email protected] +1 866 885 9794 or +1 631 224 4340 x3 +852 39070500 or +852 39070503 +7 495 98815 17 Art. No. 7001-0229

EG – declaration of conformity

EG – KONFORMITÄTSERKLÄRUNG EC - DECLARATION OF CONFORMITY CE - DECLARATION DE CONFORMITE

Anbieter / Supplier / Fournisseur:

BINDER GmbH

Anschrift / Address / Adresse:

Im Mittleren Ösch 5, D-78532 Tuttlingen

Produkt / Product / Produit:

CO2 Begasungsbrutschrank CO2 Incubators Incubateurs à CO2

Typenbezeichnung / Type / Type:

CB 60, CB 160, CB 220

Die oben beschriebenen Produkte sind konform mit folgenden EG-Richtlinien: The products described above are in conformity with the following EC guidelines: Les produits décrits ci-dessus sont conformes aux directives CE suivantes: Niederspannungsrichtlinie 2006/95/EG Low voltage directive 2006/95/EC Directive basse tension 2006/95/CE

Richtlinie 2006/95/EG des Europäischen Parlaments und des Rates vom 12. Dezember 2006 zur Angleichung der Rechtsvorschriften der Mitgliedstaaten betreffend elektrische Betriebsmittel zur Verwendung innerhalb bestimmter Spannungsgrenzen Council Directive 2006/95/EC of 12 December 2006 on the harmonization of the laws of Member States relating to electrical equipment designed for use within certain voltage limits Directive 2006/95/CE du Parlement Européen et du Conseil du 12 décembre 2006 concernant le rapprochement des législations des États membres relatives au matériel électrique destiné à être employé dans certaines limites de tension

EMV-Richtlinie 2004/108/EG EMC Directive 2004/108/EC Directive CEM 2004/108/CE

Richtlinie 2004/108/EG des Europäischen Parlaments und des Rates vom 15. Dezember 2004 zur Angleichung der Rechtsvorschriften der Mitgliedstaaten über die elektromagnetische Verträglichkeit und zur Aufhebung der Richtlinie 89/336/EWG. Directive 2004/108/EC of the European Parliament and of the Council of 15 December 2004 on the approximation of the laws of the Member States relating to electromagnetic compatibility and repealing Directive 98/336/EEC. Directive 2004/108/CE du Parlement Européen et du Conseil du 15 décembre 2004 relative au rapprochement des législations des États membres concernant la compatibilité électromagnétique et abrogeant le directive 98/336/CEE.

Die oben beschriebenen Produkte tragen entsprechend die Kennzeichnung CE. The products described above, corresponding to this, bear the CE-mark. Les produits décrits ci-dessus, en correspondance, portent l’indication CE. 1/3

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Die oben beschriebenen Produkte sind konform mit folgenden harmonisierten Normen: The products described above are in conformity with the following harmonized standards: Les produits décrits ci-dessus sont conformes aux normes harmonisées suivantes: Sicherheit / safety / sécurité: EN 61010-1:2010

Sicherheitsbestimmungen für elektrische Mess-, Steuer-, Regel- und Laborgeräte – Teil 1: Allgemeine Anforderungen (DIN EN 610101:2011, VDE 411-1:2011) Safety requirements for electrical equipment for measurement, control, and laboratory use – Part 1: General requirements (IEC 61010-1:2010, BS EN 61010-1:2010) Règles de sécurité pour appareils électriques de mesurage, de régulation et de laboratoire – Partie 1: Prescriptions générales (CEI 61010-1:2010, NF EN 61010:2011)

EN 61010-2-010:2003

Sicherheitsbestimmungen für elektrische Meß-, Steuer-, Regel- und Laborgeräte – Teil 2-010: Besondere Anforderungen an Laborgeräte für das Erhitzen von Stoffen (DIN EN 61010-2-010:2004) Safety requirements for electrical equipment for measurement, control, and laboratory use – Part 2-010: Particular requirements for laboratory equipment for the heating of materials (IEC 61010-2-10:2005, BS EN 61010-2-10:2003) Règles de sécurité pour appareils électriques de mesurage, de régulation et de laboratoire – Partie 2-010 : Prescriptions particulières pour appareils de laboratoire utilisés pour l’échauffement des matières (CEI 61010-2-10:2003, NF EN 61010-2-10:2005)

EMV / EMC / CEM: EN 61326-1:2006 + Corr. 1:2008 + Corr. 2:2010

Elektrische Mess-, Steuer-, Regel- und Laborgeräte - EMVAnforderungen - Teil 1: Allgemeine Anforderungen (DIN EN 613261:2006 + Berichtigung 1:2008 + Berichtigung 2:2011) Electrical equipment for measurement, control and laboratory use EMC requirements - Part 1: General requirements (IEC 61326-1:2005 + Corr. 1:2008 + Corr. 2:2010, BS EN 61326-1:2006+ A1:2008) Matériel électrique de mesure, de commande et de laboratoire Exigences relatives à la CEM - Partie 1: Exigences générales (CEI 61326-1:2005 + AC1:2008, NF EN 61326-1:2006 mod.)

EN 61326-2-2:2006

Elektrische Mess-, Steuer-, Regel- und Laborgeräte – EMVAnforderungen. Teil 2-2: Besondere Anforderungen - Prüfanordnung, Betriebsbedingungen und Leistungsmerkmale für ortsveränderliche Prüf-, Mess- und Überwachungsgeräte in NiederspannungsStromversorgungsnetzen. (DIN EN 61326-2-2:2006) Electrical equipment for measurement, control and laboratory use – EMC requirements. Part 2-2: Particular requirements - Test configurations, operational conditions and performance criteria for portable test, measuring and monitoring equipment used in low-voltage distribution systems. (IEC 61326-2-2:2005, BS EN 61326-2-2:2006) Matériel électrique de mesure, de commande et de laboratoire – Exigences relatives à la CEM. Partie 2-2: Exigences particulières Configurations d’essai, conditions de fonctionnement et critères d’aptitude à la fonction des matériels portatifs d’essai, de mesure et de surveillance utilisés dans des systèmes de distribution basse tension. (CEI 61326-2-2:2005 + AC1:2007, NF EN 61326-2-2:2006)

2/3 CB (E6) 09/2013

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D-78532 Tuttlingen, 20.07.2012 BINDER GmbH

P. M. Binder

B. Hofmann

Geschäftsführender Gesellschafter Managing Director Directeur général

Leiter F & E Director R & D Chef de service R&D

3/3

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Product registration

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Contents EG – declaration of conformity ... 2 Product registration ... 5

SAFETY ... 10

1.1 1.2

Legal considerations ... 10 Structure of the safety instructions ... 10 1.2.1 Signal word panel ... 10 1.2.2 Safety alert symbol ... 11 1.2.3 Pictograms ... 11 1.2.4 Word message panel structure ... 12 1.3 Localization / position of safety labels on the unit ... 12 1.4 Type plate... 14 1.5 General safety instructions on installing and operating the CO2 incubator... 15 1.6 Precautions when working with gases ... 16 1.7 Precautions when handling gas cylinders ... 18 1.8 Intended use ... 18

UNIT DESCRIPTION ... 19

2.1 2.2 2.3 2.4

Unit overview ... 20 Instrument panel ... 20 Inner chamber ... 21 Control panel on the rear of the unit ... 23

COMPLETENESS OF DELIVERY, TRANSPORTATION, STORAGE, AND INSTALLATION... 24

3.1 3.2 3.3 3.4

Unpacking, and checking equipment and completeness of delivery ... 24 Guidelines for safe lifting and transportation... 24 Storage ... 25 Location of installation and ambient conditions ... 25

INSTALLATION AND CONNECTIONS ... 29

4.1 4.2 4.3

Shelves... 29 Permadry™ water pan ... 29 Gas connections ... 30 4.3.1 Connection of the CO2 gas cylinder ... 31 4.3.2 Connection of the O2 gas cylinder (unit with O2 control)... 32 4.3.3 Connection of the N2 gas cylinder (unit with O2 control) ... 33 4.3.4 Connecting the gas hose to the unit rear (for CO2, O2, N2) ... 34 4.3.5 Gas cylinder connection kits (option) ... 35 4.4 Electrical connection ... 36 4.5 Handling and aligning the multiple-divided inner glass door, gas proof (optional equipment) ... 37

START UP ... 38

5.1 5.2

Equilibration time ... 38 Factory settings ... 38

FUNCTIONAL OVERVIEW OF THE T4.12 CHAMBER CONTROLLER ... 39

6.1

Menu structure ... 40 6.1.1 General menu ... 40 6.1.2 Quick menu ... 41 6.1.3 User menu ... 42 6.2 Operating modes ... 43 6.2.1 Activating the “control off” mode or change to “fixed value” operating mode ... 43

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Deactivating the O2 control and O2/N2 pressure alarms (unit with O2 control) ... 45 6.3.1 Required gas supply of the unit with O2 control ... 45 6.3.2 Activating / deactivating the O2 control and O2 / N2 pressure alarms ... 46 6.4 Humidity control of the Permadry™ system... 47 6.5 Turning on / off the interior socket voltage (with optional interior socket) ... 48 6.6 Performance during and after power failure... 48 6.7 Information ... 49 6.3

SETPOINT ENTRY ... 50

7.1 7.2 7.3 7.4

Setting ranges ... 50 Note when setting high gas concentrations ... 50 Entering the setpoints via “quick menu” ... 52 Entering the setpoints via general menu... 54

KEY LOCK ... 56

8.1 8.2 8.3

Directly activating the key lock ... 57 Automatic key lock ... 57 Changing the password for unlocking the key lock ... 59

GENERAL CONTROLLER SETTINGS ... 60

9.1 9.2 9.3 9.4 9.5 9.6 9.7 9.8 9.9 9.10 9.11 9.12

Setup wizard ... 61 Date and time settings ... 61 Selecting the menu language of the T4.12 controller ... 63 Setting display brightness ... 63 Changing the temperature unit... 64 Defining the data recording rate ... 64 Factory reset ... 65 Network configuration ... 65 Display of the network configuration ... 69 MAC Address ... 69 RS 422 address (with optional RS 422 interface) ... 70 Display and entry of the device configuration – for service purpose ... 71

10. DATA TRANSFER VIA USB INTERFACE... 72 10.1 Exporting data to USB drive ... 72 10.2 Importing data from USB drive ... 73

11. NOTIFICATIONS AND ALARMS ... 74 11.1 Notifications overview ... 74 11.2 Alarm messages overview ... 74 11.3 Alarm status ... 76 11.4 Confirming an active “set” alarm ... 77 11.5 Alarm configuration and overview ... 78 11.5.1 List of active alarms ... 78 11.5.2 History – list of all alarms ... 79 11.5.3 Activating, deactivating, and testing the alarm buzzer ... 80 11.5.4 Activating / deactivating all alarm functions ... 80 11.5.5 Setting the delay time after opening the door ... 81 11.5.6 Tolerance ranges and alarm delay times... 81 11.6 Zero-voltage relay alarm output ... 86

12. EVENT LIST ... 87 13. GRAPHICAL DISPLAY OF THE MEASURED VALUES ... 88 13.1 Setting the sampling rate ... 88 13.2 Defining the display range... 89 13.3 Selecting the parameters ... 90

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14. TEMPERATURE SAFETY DEVICES ... 91 14.1 Overtemperature protective device (class 1) ... 91 14.2 Overtemperature safety controller (temperature safety device class 3.1) ... 91 14.2.1 Safety controller modes ... 91 14.2.2 Setting the safety controller ... 92

15. OPTIONS ... 95 15.1 Communication software APT-COM™ 3 DataControlSystem (option) ... 95 15.2 RS 422 interface (option) ... 95 15.3 Silicone access ports 30 mm / 1.18 in, closable from both sides with silicon plugs (option) ... 95 15.4 Interior socket 230V (option) ... 96 15.5 Analog outputs for temperature and CO2 (option) ... 98 15.6 Access port for extra-low voltage (option)... 98 15.7 BINDER Gas Supply Service – External bottle changer for CO2, N2 or O2 (option) ... 99 15.8 Stands ... 100 15.8.1 Stacking stand (option) ... 100 15.8.2 Stacking adapter for direct thermal decoupled stacking (option) ... 100 15.8.3 Base on castors (option) ... 100

16. REFERENCE MEASUREMENTS ... 101 16.1 CO2 reference measuring ... 101 16.1.1 Measuring CO2 concentration indirectly via the pH of the cell medium ... 101 16.1.2 Measuring CO2 directly via chemical indicator tubes... 102 16.1.3 Measuring CO2 directly with an electronic infrared measuring device ... 103 16.2 Temperature reference measurement ... 103

17. AVOIDING MICROBIAL CONTAMINATION... 103 17.1 17.2 17.3 17.4 17.5

Cells and media ... 103 Laboratory conditions / equipment around the incubator ... 103 Working and behavior in the lab ... 104 Chamber design and equipment of the CO2 incubator ... 104 Handling the CO2 incubator ... 105

18. CLEANING, DECONTAMINATION / DISINFECTION, AND STERILIZATION .. 107 18.1 Cleaning ... 107 18.2 Decontamination / chemical disinfection of the CO2 incubator ... 108 18.3 Hot-air sterilization at 180 °C / 356 °F ... 110 18.3.1 Overview ... 110 18.3.2 Performing a hot-air sterilization ... 111 18.3.3 Aborting the hot-air sterilization prematurely ... 113 18.3.4 End of the sterilization cycle ... 115

19. MAINTENANCE AND SERVICE ... 116 19.1 19.2 19.3 19.4 19.5

Maintenance intervals, service ... 116 Checking the air jacket heating fan ... 116 Checking the humidity system fan ... 117 Gas inlet fine filter ... 117 Sending the unit back to BINDER GmbH ... 117

20. DISPOSAL... 118 20.1 Disposal of the transport packing ... 118 20.1.1 Outer unit packing ... 118 1.1.1 Packing inside the unit and equipment ... 118 20.2 Decommissioning ... 119 20.3 Disposal of the unit in the Federal Republic of Germany ... 119 20.4 Disposal of the unit in the member states of the EC except for the Federal Republic of Germany 120 20.5 Disposal of the unit in non-member states of the EC ... 121

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21. TROUBLESHOOTING ... 122 21.1 21.2 21.3 21.4 21.5 21.6 21.7

Heating ... 122 Gas cylinder pressure too low ... 123 Gas concentration ... 125 Sterilization ... 127 Humidity ... 127 Controller ... 129 Open door ... 129

22. TECHNICAL DESCRIPTION ... 130 22.1 Factory calibration and adjustment ... 130 22.2 Over current protection ... 130 22.3 Definition of usable volume ... 130 22.4 CB technical data ... 131 22.5 Equipment and Options ... 133 22.6 Spare parts and accessories ... 134 22.7 Important conversion data for non-SI units ... 135 22.8 Conversion table for gas inlet pressures, bar – psi ... 135 22.9 Dimensions CB 60 ... 136 22.10 Dimensions CB 160 ... 137 22.11 Dimensions CB 220 ... 138

23. CONTAMINATION CLEARANCE CERTIFICATE ... 139 23.1 For units located outside North America and Central America ... 139 23.2 For units in North America and Central America ... 142

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Dear customer, For the correct operation of the CO2 incubator CB, it is important that you read this operating manual completely and carefully and observe all instructions as indicated. Failure to read, understand and follow the instructions may result in personal injury. It can also lead to damage to the unit and/or poor equipment performance

Safety

This operating manual is part of the components of delivery. Always keep it handy for reference. The device should only be operated by laboratory personnel especially trained for this purpose and familiar with all precautionary measures required for working in a laboratory. To avoid injuries and damage observe the safety instructions of the operating manual.

WARNING Failure to observe the safety instructions. Serious injuries and unit damage.  Observe the safety instructions in this operating manual.  Carefully read the complete operating instructions of the CO2 incubator CB.

1.1

Legal considerations

This operating manual is for informational purposes only. It contains information for installing, start-up, operation and maintenance of the product. Note: the contents and the product described are subject to change without notice. Understanding and observing the instructions in this operating manual are prerequisites for hazard-free use and safety during operation and maintenance. In no event shall BINDER be held liable for any damages, direct or incidental arising out of or related to the use of this manual. This operating manual cannot cover all conceivable applications. If you would like additional information, or if special problems arise that are not sufficiently addressed in this manual, please ask your dealer or contact us directly by phone at the number located on page one of this manual Furthermore, we emphasize that the contents of this operating manual are not part of an earlier or existing agreement, description, or legal relationship, nor do they modify such a relationship. All obligations on the part of BINDER derive from the respective purchase contract, which also contains the entire and exclusively valid statement of warranty administration. The statements in this manual neither augment nor restrict the contractual warranty provisions.

1.2

Structure of the safety instructions

In this operating manual, the following safety definitions and symbols indicate dangerous situations following the harmonization of ISO 3864-2 and ANSI Z535.6.

1.2.1

Signal word panel

Depending on the probability of serious consequences, potential dangers are identified with a signal word, the corresponding safety color, and if appropriate, the safety alert symbol.

DANGER Indicates an imminently hazardous situation that, if not avoided, will result in death or serious (irreversible) injury.

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WARNING Indicates a potentially hazardous situation which, if not avoided, could result in death or serious (irreversible) injury.

CAUTION Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor (reversible) injury.

CAUTION Indicates a potentially hazardous situation which, if not avoided, may result in damage to the product and/or its functions or of a property in its proximity.

1.2.2

Safety alert symbol Use of the safety alert symbol indicates a risk of injury. Observe all measures that are marked with the safety alert symbol in order to avoid death or injury.

1.2.3

Pictograms

Warning signs

Electrical hazard

Hot surface

Explosive atmosphere

Stability hazard

Lifting hazard

Gas cylinders

Suffocation hazard

CO2 suffocation and poisoning hazard

Explosive substances

Fire promoting agents

Harmful substances

Risk of corrosion and / or chemical burns

Biohazard

Pollution Hazard

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Mandatory action signs

Mandatory regulation

Read operating instructions

Disconnect the power plug

Environment protection Prohibition signs

Wear protective gloves

Wear safety goggles

Do NOT touch

Do NOT spray with water

Do NOT climb

Lift with several persons

Information to be observed in order to ensure optimum function of the product.

1.2.4

Word message panel structure

Type / cause of hazard. Possible consequences.  Instruction how to avoid the hazard: prohibition  Instruction how to avoid the hazard: mandatory action Observe all other notes and information not necessarily emphasized in the same way, in order to avoid disruptions that could result in direct or indirect injury or property damage.

1.3

Localization / position of safety labels on the unit

The following labels are located on the unit: Pictograms (Warning signs)

Service label

Hot surface Risk of injury • on the outer door: CB-UL only • above the access ports (option)

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CO2 -Incubator CB

CO2 -Incubator CB-UL

Figure 1: Position of labels on the unit

Keep safety labels complete and legible. Replace safety labels that are no longer legible. Contact BINDER service for these replacements.

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1.4

Type plate

Position of type plate: left unit side (seen from front), at the bottom in the middle Nominal temperature 187 °C 369°F Enclosure protection IP 20 Temp. safety device DIN 12880 Class 3.1 Art. No. 9040-0092 Project No.

1,30 kW 200-240 V 1 N ~ 5,7 A 50/60 Hz US PATS 4585923 / 5222612 / 5309981 5405194 / 5601143 / 5773287 / 6079403 D 78532 Tuttlingen / Germany Tel. + 49 (0) 7462/ 2005-0 Internet: www.binder-world.com

CB 160 Serial No. 00-00000 Made in Germany

Figure 2: Type plate (example of CB 160 regular unit) Indications of the type plate BINDER CB 160 Serial No. 00-00000 Nominal temperature 1 °C 369 °F Enclosure protection IP 20 Temp. safety device DIN 12880 Class 3.1 Art. No. 9040-0092 Project No. --1,30 kW 200-240 V 1 N ~ 5,7 A 50/60 Hz

Information Manufacturer: BINDER GmbH Model CB 160 Serial No. 00-00000

Symbol on the type plate

Information

Nominal temperature IP type of protection 20 acc. to EN 60529 Temperature safety device acc. to standard DIN 12880 Temperature safety device, class 3.1 Art. No. 9040-0092 (Special application acc. to project no.) Nominal power 1,30 kW Nominal voltage 200-240 V ± 10%, single-phase unit Nominal current 5,7 Amp Power frequency 50/60 Hz

CE conformity marking Electrical and electronic equipment manufactured / placed on the market in the EC after 13 August 2005 and to be disposed of in separate collection according to directive 2002/96/EC on waste electrical and electronic equipment (WEEE). The equipment is certified in the GOST R certification system of GOSTSTANDARD Russia. ®

(CB-UL only) LABORATORY EQUIPMENT 43KM

CB (E6) 09/2013

The equipment is certified by Underwriters Laboratories Inc. nd according to standards CAN/CSA-C22.2 No. 61010-1, 2 Edition, 2004-07 (Electrical Equipment for Measurement, Control, and Laboratory Use; Part 1: General Requirements); UL nd 61010-1, 2 Edition, 2005-07-22 (Electrical Equipment for Measurement, Control, and Laboratory Use; Part 1: General rd Requirements); IEC 61010-1:2010, 3 Edition and IEC 61010-210:2003 (Particular Requirements for Laboratory Equipment for the heating of materials).

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1.5

General safety instructions on installing and operating the CO2 incubator

With regard to operating the CO2 incubator CB and to the installation location, please observe the guideline BGI/GUV-I 850-0 on safe working in laboratories (formerly BGR/GUV-R 120 or ZH 1/119 laboratory guidelines issued by the employers’ liability insurance association) (for Germany). BINDER GmbH is only responsible for the safety features of the unit provided skilled electricians or qualified personnel authorized by BINDER perform all maintenance and repair, and if components relating to chamber safety are replaced in the event of failure with original spare parts. To operate the unit, use only original BINDER accessories or accessories from third-party suppliers authorized by BINDER. The user is responsible for any risk caused by using unauthorized accessories.

CAUTION Danger of overheating. Damage to the unit. ∅ Do NOT install the unit in unventilated recesses.  Ensure sufficient ventilation for dispersal of the heat. Do not operate the CO2 incubator CB in hazardous locations.

DANGER Explosion hazard. Danger of death. ∅ Do NOT operate the unit in potentially explosive areas.  KEEP explosive dust or air-solvent mixtures AWAY from the unit. The CO2 incubator CB does not dispose of any measures of explosion protection.

DANGER Explosion hazard. Danger of death. ∅ Do NOT introduce any substance into the CO2 incubator which is combustible or explosive at working temperature. ∅ NO explosive dust or air-solvent mixture in the inner chamber. Any solvent contained in the charging material must not be explosive or inflammable. I.e., irrespective of the solvent concentration in the steam room, NO explosive mixture with air must form. The temperature inside the chamber must lie below the flash point or below the sublimation point of the charging material. Familiarize yourself with the physical and chemical properties of the charging material, as well as the contained moisture constituent and its behavior with the addition of heat energy. Familiarize yourself with any potential health risks caused by the charging material, the contained moisture constituent or by reaction products which may arise during the temperature process. Take adequate measures to exclude such risks prior to putting the CO2 incubator into operation.

DANGER Electrical hazard. Danger of death. ∅ The unit must NOT become wet during operation or maintenance. The CO2 incubators were produced in accordance with VDE regulations and were routinely tested in accordance to VDE 0411-1 (IEC 61010-1).

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CAUTION The glass doors and the inner chamber will become hot during operation. Danger of burning. ∅ Do NOT touch the glass doors, the inner surfaces or the charging material during operation.

WARNING Stability hazard. Danger of injury. Damage to the unit and the charging material. Housing cover breakaway. ∅ Do NOT climb on the lower housing cover. ∅ Do NOT load the lower housing cover with heavy objects while the unit door is open.

1.6

Precautions when working with gases

Notes on handling carbon dioxide (CO2) Carbon dioxide (CO2) in high concentrations is hazardous to health. It is colorless and almost odorless and therefore practically imperceptible. Vent out any CO2 gas that may escape via good room ventilation or a suitable connection to an exhaust system. We recommend installing a CO2 warning system

WARNING High concentration of CO2 (> 4 Vol.-%). Risk of death by suffocation. Danger of poisoning. ∅ Do NOT set up units in non-ventilated recesses.  Ensure technical ventilation measures.  Observe the relevant regulations for handling CO2.

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Unit with O2 control: Notes on handling oxygen (O2) Oxygen (O2) is colorless and almost odorless and therefore practically imperceptible. It promotes burns, which can proceed explosively. There is a fire hazard for flammable oxygenated materials, e.g. clothes and hair. O2 is heavier than air and may accumulate in low-lying areas.

WARNING High concentration of O2 (> 21 % O2). Fire and explosion hazard through contact of combustible materials with O2. Risk of burns and other injuries. ∅ Do NOT set up units in non-ventilated recesses.  Ensure technical ventilation measures.  Observe the relevant regulations for handling O2. Take appropriate measures to prevent oxygen enrichment and fire and explosion hazards in areas where oxygen enrichment is possible. General information for safe handling of oxygen: • Make sure training of personnel on hazards of oxygen enrichment and necessary safety measures. • Make sure adequate labeling of all oxygen equipment and facilities. • Make sure gas tightness of all gas connections by checking them for leaks (e.g. with leak spray or diluted soap solution). • Close the main valve of the source of oxygen after work when not using the chamber. • Never lubricate O2 equipment with oil or fat. Use only materials and spare parts which are approved for use with oxygen. • Regularly inspect fire extinguishers for proper condition. • Set up emergency showers where oxygen enrichment is possible. • Strictest smoking ban and no ignition sources in areas where oxygen enrichment is possible. • Make sure good ventilation of areas where oxygen enrichment is possible (location of the chamber and/or O2 cylinders. • Persons who may have been in a possibly oxygen-enriched atmosphere must keep away from ignition sources (flames, cigarettes, etc.) and ventilate their clothes at least 15 minutes. • Always keep emergency routes free. Unit with O2 control: Notes on handling nitrogen (N2) Nitrogen (N2) in high concentrations is hazardous to health. It is colorless and almost odorless and therefore practically imperceptible. Any N2 gas that may escape must be safely led out via good room ventilation or a suitable connection to an exhaust system.

WARNING High concentration of N2. Risk of death by suffocation. ∅ Do NOT set up units in non-ventilated recesses.  Ensure technical ventilation measures.  Observe the relevant regulations for handling N2.

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1.7

Precautions when handling gas cylinders General information for safe handling of gas cylinders: • Store and use gas cylinders only in well-ventilated locations. • Open the gas cylinder valve slowly to avoid pressure surges. • Secure gas cylinders during storage and use against falling (chaining). • Transport gas cylinders with a cylinder cart, do not carry, roll, or throw them. • Always close the valve even with apparently empty cylinders; screw on the cap when not in use. Return gas cylinders with the valve closed. • Do not open gas cylinders by force. Mark them when damaged. • Protect gas cylinders against fire, e.g. do not store together with flammable liquids. • Observe relevant regulations for dealing with gas cylinders.

Secure the gas cylinders against falling and other mechanical damage.

WARNING Safety valve tearing off. Sudden release of the stored pressure energy. Risk of injury.  Secure gas cylinders against falling (chaining).  Transport gas cylinders with a cylinder cart. The valve of the gas cylinder always must be closed before screwing on or unscrewing the gas hose.

WARNING Opening the cylinder valve when the cylinder is not connected. Sudden release of the stored pressure energy. Risk of injury.  Close the gas cylinder valve before connecting or removing the gas hose. After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or diluted soap solution).

1.8

Intended use

Series CB incubators are suitable for the cultivation of mammal cells under typical conditions of approx. 37 °C / 98.6°F. The incubator permits setting defined pH conditions by common NaHCO3 buffer systems of commercial cell media by keeping an exact CO2 atmosphere inside. CB incubators guarantee high humidity inside to avoid osmolarity increasing caused by the evaporation of the cell media. With the unit with O2 control, a variable oxygen atmosphere can additionally influence the growth of the cells. Following the instructions in this operating manual and conducting regular maintenance work (chap. 21.1) are part of the intended use. Other applications are not approved. WARNING: If customer should use a BINDER chamber running in non-supervised continuous operation, we strongly recommend in case of inclusion of irrecoverable specimen or samples to split such specimen or samples and store them in at least two chambers, if this is feasible.

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Unit description

The CO2 incubators CB are equipped with a multifunctional microprocessor display controller for temperature, CO2, and O2 (unit with O2 control) levels and a digital display accurate to one-tenth of a degree resp. 0.1 vol.-% The inner chamber, the pre-heating chamber and the inside of the doors are all made of stainless steel (material no. 1.4301 (V2A) in Germany). The inner surfaces are smooth and therefore easy to clean. The inner chamber is deep-drawn from one piece, polished (suitable for pharmaceutical applications) and has no welds or inaccessible corners. The hinges and the seal of the inner glass door are glued from the outside to aid cleaning of the inner chamber. When operating the chamber at high temperatures (sterilization), the impact of the oxygen in the air may cause discoloration of the metallic surfaces (yellowish-brown or blue) by natural oxidation processes. These colorations are harmless and will in no way impair the function or quality of the unit. The perforated shelves are also made of stainless steel. You can insert a maximum of 3 (CB 60), 6 (CB 160), resp. 8 (CB 220) shelves. The housing is RAL 7035 powder-coated. All corners and edges are also completely coated. The heating system of the CO2 incubator permits hot-air auto-sterilization at a setpoint of 187.5 °C / 369.5°F. Thus, a temperature of 180 °C / 356°F is maintained for at least 30 minutes on all internal surfaces, resulting in sterilization of the entire inner chamber. Therefore, this procedure meets all international guidelines regarding hot air sterilization, e.g. AAMI ST63, DIN 58947, European Pharmacopoeia. Thanks to the standard safety device (class 3.1 according to DIN 12880), the set temperature is maintained in case of failure. The gas enters the chamber via a fine filter (aseptic filter) with a high filtration efficiency that also filters the smallest particles. A highly precise, drift-free CO2 infrared measuring system in combination with the permanent mixture of CO2 gas through a special proprietary gas mixing head developed by BINDER allows precise and constant CO2 concentrations for long periods. This creates optimum growth conditions for cultures. The CO2 incubator is also available with O2 control in addition to CO2 control. CO2 and O2 sensors Fast reaction times, maximum accuracy and selectivity characterize the CO2 measuring procedure of the CB incubator series. The accuracy of the CO2 measuring system is based on a double-beam infrared measuring cell with NDIR (non-dispersive infrared) sensor, which continuously regulates to a reference value. Therefore, disturbance variables and aging phenomena in the measuring system are almost completely eliminated, so that this measuring system, in contrast to other measuring procedures, remains practically drift-free between calibrations and is entirely selective for CO2 and humidity. The sensor is built into the incubator and can be sterilized. The O2 sensor is a semiconductor gas sensor with ZrO2 ceramic. The accuracy of the indicated values of CO2 and O2 (unit with O2 control) depends on the ambient air pressure (approx. 0.08 vol.-% per 10 mbar / 0.15 psi). To compensate for this effect in the CO2 measurement, the controller measures the ambient air pressure and automatically includes it in the calculation. The CO2 incubators CB are equipped with an Ethernet interface for computer communication, e.g. with the communication software APT-COM™ 3 DataControlSystem (option, chap. 17.1). For further options, see chap. 24.4. Temperature range: 7 °C / 12.6 °F above ambient temperature up to +60 °C / 140 °F CO2 range: 0 vol.-% up to 20 vol.-%) O2 range (unit with O2 control): 0.2 vol.-% up to 95 vol.-%

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2.1

Unit overview Instrument panel with microprocessor controller T4.12 and USB interface

Door handle

Unit door

Main power switch

Figure 3: CO2 incubator CB (example: model CB 160)

2.2

Instrument panel

5,7" controller display

Context-sensitive buttons USB interface Operating button Pilot lamp: ready for operation

Figure 4: Instrument panel with microprocessor controller T4.12 and USB interface

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2.3

Inner chamber (A)

(B) (C) (D) (E) (G)

(H)

FRONT

(I) (J) (K) (L) Figure 5: CB 160 with O2 control and options

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(B) (C) (D) (D2) (F) (G)

(E)

Figure 6: CB 160 / CB 220 with O2 control and options (A)

Instrument panel with microprocessor controller T4.12, indicating temperature and CO2 as well as O2 (unit with O2 control)”

(B)

Connection socket for extra-low voltage supply (option, chap. 17.6)

(C)

CO2 and humidity sensor

(D)

Gas mixing head CO2

(D2) Additional gas mixing head O2/ N2 (unit with O2 control) (E)

Pt 100 temperature probe

(F)

O2 sensor (unit with O2 control)

(G)

Internal socket 230V (max. 3 A) (option, chap. 17.4)

(H)

Perforated shelves, made of stainless steel

(I)

Measuring access port

(J)

Inner glass doors

(K)

Permadry™ water pans

(L)

Lower housing cover

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2.4

Control panel on the rear of the unit (1)

(2)

(3)

(7)

(8)

(9)

(6)

(4)

(5)

(10)

Figure 7: Rear control panel CB with O2 control and options (1)

Miniature fuse

(2)

External socket for extra-low voltage supply (option for CB 160 / CB 220, chap. 17.6)

(3)

DIN socket for analog outputs 4-20 mA (option, chap. 17.5)

(4)

Quick acting closure socket for CO2

(5)

Quick acting closure socket for O2 (unit with O2 control)

(6)

Quick acting closure socket for N2 (unit with O2 control)

(7)

Socket for IEC connector plug for power cable

(8)

Ethernet interface for computer communication

(9)

DIN-socket for zero-voltage relay alarm outputs

(10)

RS 422 interface for computer communication (option)

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Completeness installation

delivery,

transportation,

storage,

3.1

Unpacking, and checking equipment and completeness of delivery

and

After unpacking, please check the unit and its optional accessories, if any, based on the delivery receipt for completeness and for transportation damage. Inform the carrier immediately if transportation damage has occurred. The final tests of the manufacturer may have caused traces of the shelves on the inner surfaces. This has no impact on the function and performance of the unit. Please remove any transportation protection devices and adhesives in/on the unit and on the doors and remove the operating manuals and accessory equipment. Remove any protective lamination sheet on the inner metal surfaces prior to commissioning.

CAUTION Sliding or tilting of the unit. Damage to the unit. Risk of injury by lifting heavy loads.  Do NOT lift or transport the unit using the door handle, the door or the lower housing.  Lift the unit from the pallet at the four lower corners with the aid of four people.

If you need to return the unit, please use the original packing and observe the guidelines for safe lifting and transportation (chap. 3.2). For disposal of the transport packing, see chap. 22.1. Note on second-hand units (Ex-Demo-Units): Second-hand units are units that were used for a short time for tests or exhibitions. They are thoroughly tested before resale. BINDER ensures that the chamber is technically sound and will work flawlessly. Second-hand units are marked with a sticker on the unit door. Please remove the sticker before commissioning the unit.

3.2

Guidelines for safe lifting and transportation

After operation, please observe the guidelines for temporary decommissioning (chap. 22.2). Empty the Permadry™ water pan before moving the incubator. In case of any spilling of the contents, shut down the incubator and dry it out carefully and completely.

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CAUTION Sliding or tilting of the unit. Damage to the unit. Risk of injury by lifting heavy loads.  Transport the unit in its original packaging only.  For moving or shipping, secure the unit with transport straps. ∅ Do NOT lift or transport the unit using the door handle, the door or the lower housing.  Lift the unit at the four lower corners with the aid of 4 people and place it on a rolling pallet.  Move the unit to the desired location and lift it from the rolling pallet with the aid of four people. • Permissible ambient temperature range during transport: 10 °C / 14 °F to +60 °C / 140 °F. You can order transport packing for moving or shipping purposes from BINDER service.

3.3

Storage

Intermediate storage of the unit is possible in a closed and dry room. Observe the guidelines for temporary decommissioning (chap. 22.2). • Permissible ambient temperature range during storage: -10 °C / 14 °F to +60 °C / 140 °F. • Permissible ambient humidity: max. 70 % r.H., non-condensing When after storage in a cold location you transfer the unit to its warmer installation site, condensation may form. Before start-up, wait at least one hour until the chamber has attained ambient temperature and is completely dry.

3.4

Location of installation and ambient conditions

Notes on the location of installation Set up the CO2 incubator on a flat, even surface, free from vibration and in a well-ventilated, dry location. The chambers are designed for setting up inside a building (indoor use). Freestanding CB incubators are suitable for installation on tables or on the optionally available stand (height 200 mm / 0.5 ft). Note: The site of installation must be capable of supporting the unit’s weight (see technical data, chap.24.3). Align the unit using a spirit level to ensure even covering of the cell-cultures with the medium. For this purpose, manually adjust the four incubator feet. CO2 incubators can be stacked on top of each other (two units maximum). For safe stacking that is easy to maintain, use the original BINDER stacking stand (chap. 17.9.1) or the stacking adapter (chap. 17.9.2).

CAUTION Sliding of stacked unit. Damage to the units.  When stacking, use rubber pads (Art. No. 8012-0376) for the feet of the upper incubator. To completely separate the unit from the power supply, you must disconnect the power plug. Install the unit in a way that the power plug is easily accessible and can be easily pulled in case of danger. In order to avoid contamination, never place the unit directly on the floor.

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CAUTION Danger of overheating. Damage to the unit. ∅ Do NOT set up units in non-ventilated recesses.  Ensure sufficient ventilation for dispersal of the heat. Do not install or operate the CO2 incubator CB in potentially explosive areas.

DANGER Explosion hazard. Danger of death. ∅ Do NOT operate the unit in potentially explosive areas.  KEEP explosive dust or air-solvent mixtures AWAY from the vicinity of the unit. Ambient conditions • Permissible ambient temperature range for operation: +18 °C / 64.4 °F to +30 °C / 86 °F At elevated ambient temperature values, fluctuations in temperature can occur. •

Ideal ambient temperature: at least 7 °C / 12.6 °F below the intended working temperature. E.g., working temperature 37 °C / 98.6 °F = ambient temperature 30 °C / 86 °F and lower. In the event of working temperatures of less than 7 °C / 12.6 °F above the ambient temperature, the setpoint can be exceeded. The ambient temperature should not be substantially higher than the indicated ambient temperature of 22 ±3 °C / 71.6 ±5.4 °F to which the specified technical data relates. For other ambient conditions, deviations from the indicated data are possible. Avoid direct solar radiation on the unit.

• Permissible ambient humidity: 70 % r.H. max., non-condensing. •

Installation height: max. 2000 m / 6561.7 ft above sea level.

•

Wall distances: rear 100 mm / 3.94 in, sides 50 mm / 1.97 in.

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WARNING High concentration of CO2 (> 4 Vol.-%). Danger of death by suffocation. Danger of poisoning. ∅ Do NOT set up units in non-ventilated recesses.  Ensure technical ventilation measures.  Observe the relevant regulations for handling CO2. Observe the occupational exposure limit OEL for CO2 set by the national authorities (formerly maximum permitted workplace concentration). Check compliance when operating all units located in the room. 3

• OEL for Germany: 5000 ml/m (ppm) = 0,5 Vol.-% •

CO2 lost with each opening the door: about 16.4 g, i.e. 0.0084 cubic meters / 0.296 cubic feet (under normal pressure)

•

CO2 lost during 12h at 5 vol.-% without opening the door: approx. < 2 g, i.e. 0.001 cubic meter / 0.035 cubic feet (under normal pressure 1013 mbar / 14.7 psi)

An example of how to evaluate laboratory volume and air change rate: Question: Is an air change rate of 1/h sufficient for a lab with a volume of 100 cubic meters / 3,531.5 cubic feet with 10 incubators CB, opened 4 times per hour? Calculation: CO2 concentration = CO2 lost by opening the door, multiplied by 10 units, multiplied by opening the door 4 times per hour, divided by lab volume 0.0084 cubic meters x 10 x 4 div. 100 cubic meters = 0.00336, i.e. 0.336 % or 3360 ppm. 0.296 cubic feet x 10 x 4 div. 3,531.5 cubic feet = 0.00336, i.e. 0.336 % or 3360 ppm. Result: The maximum permissible value of 5000 ppm is not exceeded under these operation conditions. Unit with O2 control: Notes on handling oxygen (O2) Oxygen (O2) is colorless and almost odorless and therefore practically imperceptible. It promotes burns, which can proceed explosively. There is a fire hazard for flammable oxygenated materials, e.g. clothes and hair. O2 is heavier than air and may accumulate in low-lying areas.

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General information for safe handling of oxygen: • Make sure training of personnel on hazards of oxygen enrichment and necessary safety measures. • Make sure adequate labeling of all oxygen equipment and facilities. • Make sure gas tightness of all gas connections by checking them for leaks (e.g. with leak spray or diluted soap solution). • Close the main valve of the source of oxygen after work when not using the chamber. • Never lubricate O2 equipment with oil or fat. Use only materials and spare parts which are approved for use with oxygen. • Regularly inspect fire extinguishers for proper condition. • Set up emergency showers where oxygen enrichment is possible. • Strictest smoking ban and no ignition sources in areas where oxygen enrichment is possible. • Make sure good ventilation of areas where oxygen enrichment is possible (location of the chamber and/or O2 cylinders. • Persons who may have been in a possibly oxygen-enriched atmosphere must keep away from ignition sources (flames, cigarettes, etc.) and ventilate their clothes at least 15 minutes. • Always keep emergency routes free. Unit with O2 control: Notes on handling nitrogen (N2) Nitrogen (N2) in high concentrations is hazardous to health. It is colorless and almost odorless and therefore practically imperceptible. Any N2 gas that may escape must be safely led out via good room ventilation or a suitable connection to an exhaust system.

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Installation and connections

4.1

Shelves

You can put the shelves in different positions at the line of channel beads in the inner chamber. Hold the shelf straight and then insert it so it will go smoothly inside the unit. Permitted shelf loads: Maximum load of one single shelf: Maximum total load of all shelves:

4.2

10 kg / 22 lb 30 kg / 66 lb

Permadry™ water pan

The Permadry™ system developed by BINDER is an effective and easy to handle system that ensures high humidity inside the incubator without any condensation forming on the inner surfaces. The Permadry™ water pan consists of two pans in which the outer one is heated and the inner one cooled. With the slight difference of temperature caused by that cooling, the central pan is the specific point for condensation of the surplus humidity. Therefore, all other inner surfaces remain dry.

Figure 8: Permadry™ water pan CB 160 / CB 220

Figure 9: Permadry™ water pan CB 60

•

Put the Permadry™ water pan on the bottom of the inner chamber in a way that both notches lock into place.

•

The front side of the Permadry™ water pan is marked “FRONT”. Figure 10: Letters “FRONT” indicating the front of the Permadry™ water pan

•

Please make sure that the Permadry™ water pan has firm contact to the inner chamber bottom and rests tightly on it.

•

Fill only the outer pan with distilled, sterilized water up to the filling level marking on the edge of the inner pan. Maximum filling quantity of the outer pan: CB 60: approx. 0.7 liters, CB 160 and CB220): approx. 2 liters.

Figure 11: Filling height line of the outer basin CB 160 / CB 220

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•

We recommend cleaning and refilling the pans 2 to 3 times a week. For evacuation, remove the Permadry™ water pan.

•

We recommend using distilled, sterile water to achieve optimum growth results. Any corrosive damage that may arise following the use of water of different quality or by additives is excluded from the liability agreement.

•

If required, you can add microbiologically inhibiting substances such as copper chips, copper sulfate or ethylene diamine tetra-vinegar acid (EDTA) in a concentration of 1 to 5 mmol/l.

Empty the Permadry™ water pan before moving the incubator. In case of the contents spilling, immediately shut down the incubator and dry it carefully and completely.

4.3

Gas connections General information for safe handling of gas cylinders: • Store and use gas cylinders only in well ventilated areas. • Open the gas cylinder valve slowly to avoid pressure surges. • Secure gas cylinders during storage and use against falling (chaining). • Transport gas cylinders with a cylinder cart, do not carry, roll, or throw them. • Always close the valve even with apparently empty cylinders; screw on the cap when not in use. Return gas cylinders with the valve closed. • Do not open gas cylinders by force. Mark them when damaged. • Protect gas cylinders against fire, e.g. do not store together with flammable liquids. • Observe relevant regulations for dealing with gas cylinders.

Secure the gas cylinder against falling and other mechanical damage.

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4.3.1

Connection of the CO2 gas cylinder

Carbon dioxide (CO2) in high concentrations is hazardous to health. It is colorless and almost odorless and therefore practically imperceptible. Vent out any CO2 gas that may escape via good room ventilation or a suitable connection to an exhaust system. We recommend installing a CO2 warning system.

WARNING High concentration of CO2 (> 4 Vol.-%). Danger of death by suffocation. ∅ Do NOT set up units in non-ventilated recesses.  Ensure technical ventilation measures.  Observe the relevant regulations for handling CO2. The CO2 gas necessary for operation must have a technical grade of 99.5 %.

The gas connections must be established by qualified personnel who are trained in handling the respective gases and familiar with the required safety measures. The following steps are required: Ensuring the correct CO2 output pressure A gas supply pressure above 2.5 bar / 36 psi will result in unit damage. Use a pressure reducer and make sure to avoid any excessive outlet pressure when connecting the gas hose to the incubator. The real outlet pressure of gas cylinders, sets of gas cylinders or central gas supplies am on the second manometer must not exceed 2.5 bar / 36 psi.

CAUTION Excessive outlet pressure > 2.5 bar / 36 psi. Damage to the unit. ∅ The outlet pressure must NOT exceed the indicated value of 2.5 bar / 36 psi.  Before connecting, check the outlet pressure on the pressure reducer of the cylinder.  Adjust the outlet pressure to 2.0 bar / 29 psi above the ambient pressure. Observe the correct outlet pressure also when replacing the gas cylinders. Establishing the connection to the incubator Connect the supplied gas hose (internal diameter 6 mm / 0.24 inches) to the pressure reducer of the gas cylinders or central gas supply and secure the connection with the supplied hose clamp. Connect the pre-assembled hose nozzle of the gas hose to the quick acting closure socket (4) DN 6 on the unit rear, as described in chap. 4.3.4. Leak test After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or diluted soap solution).

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The recovery times of the gas concentrations inside the chamber after opening the door are indicated in the technical data (chap. 24.3) and refer to a connection pressure of 2.0 bar / 29 psi. Decreasing supply pressure will result in longer recovery times. Conversion table for gas inlet pressures, bar – psi, see chap. 24.7.

4.3.2

Connection of the O2 gas cylinder (unit with O2 control) Note: Do not connect the O2 cylinder or disconnect the gas supply (by pulling off the gas hose) when operating at setpoints below 19 vol.-% O2.

Oxygen (O2) is colorless and almost odorless and therefore practically imperceptible. It promotes burns, which can proceed explosively. There is a fire hazard for flammable oxygenated materials, e.g. clothes and hair. O2 is heavier than air and may accumulate in low-lying areas.

WARNUNG High concentration of O2 (> 21 % O2). Fire and explosion hazard through contact of combustible materials with O2. Risk of burns and other injuries. ∅ Do NOT set up units in non-ventilated recesses.  Ensure technical ventilation measures.  Observe the relevant regulations for handling O2. The O2 gas necessary for operation must have a technical grade of 99.5 %.

The gas connections must be established by qualified personnel who are trained in handling the respective gases and familiar with the required safety measures.

The following steps are required: Ensuring the correct O2 output pressure A gas supply pressure above 2.5 bar / 36 psi will result in unit damage. Use a pressure reducer and make sure to avoid any excessive outlet pressure when connecting the gas hose to the incubator. The real outlet pressure of gas cylinders, sets of gas cylinders or central gas supplies am on the second manometer must not exceed 2.5 bar / 36 psi.

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Establishing the connection to the incubator Connect the supplied gas hose (internal diameter 6 mm / 0.24 inches) to the pressure reducer of the gas cylinders or central gas supply and secure the connection with the supplied hose clamp. Connect the pre-assembled hose nozzle of the gas hose to the quick acting closure socket (5) DN 6 on the unit rear, as described in chap. 4.3.4. Leak test After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or diluted soap solution). The recovery times of the gas concentrations inside the chamber after opening the door are indicated in the technical data (chap. 24.3) and refer to a connection pressure of 2.0 bar / 29 psi. Decreasing supply pressure will result in longer recovery times. Conversion table for gas inlet pressures, bar – psi, see chap. 24.7.

4.3.3

Connection of the N2 gas cylinder (unit with O2 control)

Nitrogen (N2) in high concentrations is hazardous to health. It is colorless and almost odorless and therefore practically imperceptible. Any N2 gas that may escape must be safely led out via good room ventilation or a suitable connection to an exhaust system.

The gas connections must be established by qualified personnel who are trained in handling the respective gases and familiar with the required safety measures. The following steps are required: Ensuring the correct N2 output pressure A gas supply pressure above 2.5 bar / 36 psi will result in unit damage. Use a pressure reducer and make sure to avoid any excessive outlet pressure when connecting the gas hose to the incubator. The real outlet pressure of gas cylinders, sets of gas cylinders or central gas supplies am on the second manometer must not exceed 2.5 bar / 36 psi.

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