mdoles_cb_series_operating_manual_sept_2013.pdf
Page 1
Operating Manual
APT.line™ CB (E6)
CO2 - Incubators
CO2 – Incubators with O2 control
with sterilizable NDIR sensor system for CO2 and humidity
and microprocessor controller T4.12
Model
Voltage
Equipment
CB 60
230 V
9040-0088, 9140-0088
CB 60-UL
100-120V
9040-0089, 9140-0089
CB 60
230 V
with O2 control
9040-0090, 9140-0090
CB 60-UL
100-120V
with O2 control
9040-0091, 9140-0091
CB 160 (E6)
230 V
9040-0092, 9140-0092
CB 160-UL
100-120V
9040-0093, 9140-0093
CB 160 (E6)
230 V
with O2 control
9040-0094, 9140-0094
CB 160-UL
100-120V
with O2 control
9040-0095, 9140-0095
CB 160
230 V
with divided glass door
9040-0100, 9140-0100
CB 160-UL
100-120V
with divided glass door
9040-0101, 9140-0101
CB 160
230 V
with O2 control
with divided glass door
9040-0102, 9140-0102
CB 160-UL
100-120V
with O2 control
with divided glass door
9040-0103, 9140-0103
CB 220
230 V
9040-0096, 9140-0096
CB 220-UL
100-120V
9040-0097, 9140-0097
CB 220
230 V
with O2 control
9040-0098, 9140-0098
CB 220-UL
100-120V
with O2 control
9040-0099, 9140-0099
BINDER GmbH
Address
Tel.
Fax
Internet
Service Hotline
Service Fax
Service E-Mail
Service Hotline USA
Service Hotline Asia Pacific
Service Hotline Russia and CIS
Issue 09/2013
Art. No.
Post office box 102
D-78502 Tuttlingen
+49 7462 2005 0
+49 7462 2005 100
http://www.binder-world.com
info@binder-world.com
+49 7462 2005 555
+49 7462 2005 93 555
service@binder-world.com
+1 866 885 9794 or +1 631 224 4340 x3
+852 39070500 or +852 39070503
+7 495 98815 17
Art. No. 7001-0229
Page 2
EG – declaration of conformity
EG – KONFORMITÄTSERKLÄRUNG
EC - DECLARATION OF CONFORMITY
CE - DECLARATION DE CONFORMITE
Anbieter / Supplier / Fournisseur:
BINDER GmbH
Anschrift / Address / Adresse:
Im Mittleren Ösch 5, D-78532 Tuttlingen
Produkt / Product / Produit:
CO2 Begasungsbrutschrank
CO2 Incubators
Incubateurs à CO2
Typenbezeichnung / Type / Type:
CB 60, CB 160, CB 220
Die oben beschriebenen Produkte sind konform mit folgenden EG-Richtlinien:
The products described above are in conformity with the following EC guidelines:
Les produits décrits ci-dessus sont conformes aux directives CE suivantes:
Niederspannungsrichtlinie
2006/95/EG
Low voltage directive
2006/95/EC
Directive basse tension
2006/95/CE
Richtlinie 2006/95/EG des Europäischen Parlaments und des
Rates vom 12. Dezember 2006 zur Angleichung der
Rechtsvorschriften der Mitgliedstaaten betreffend elektrische
Betriebsmittel zur Verwendung innerhalb bestimmter
Spannungsgrenzen
Council Directive 2006/95/EC of 12 December 2006 on the
harmonization of the laws of Member States relating to electrical
equipment designed for use within certain voltage limits
Directive 2006/95/CE du Parlement Européen et du Conseil du 12
décembre 2006 concernant le rapprochement des législations des
États membres relatives au matériel électrique destiné à être
employé dans certaines limites de tension
EMV-Richtlinie
2004/108/EG
EMC Directive
2004/108/EC
Directive CEM
2004/108/CE
Richtlinie 2004/108/EG des Europäischen Parlaments und des
Rates vom 15. Dezember 2004 zur Angleichung der
Rechtsvorschriften der Mitgliedstaaten über die
elektromagnetische Verträglichkeit und zur Aufhebung der
Richtlinie 89/336/EWG.
Directive 2004/108/EC of the European Parliament and of the
Council of 15 December 2004 on the approximation of the laws of
the Member States relating to electromagnetic compatibility and
repealing Directive 98/336/EEC.
Directive 2004/108/CE du Parlement Européen et du Conseil du
15 décembre 2004 relative au rapprochement des législations des
États membres concernant la compatibilité électromagnétique et
abrogeant le directive 98/336/CEE.
Die oben beschriebenen Produkte tragen entsprechend die Kennzeichnung CE.
The products described above, corresponding to this, bear the CE-mark.
Les produits décrits ci-dessus, en correspondance, portent l’indication CE.
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Die oben beschriebenen Produkte sind konform mit folgenden harmonisierten Normen:
The products described above are in conformity with the following harmonized standards:
Les produits décrits ci-dessus sont conformes aux normes harmonisées suivantes:
Sicherheit / safety / sécurité:
EN 61010-1:2010
Sicherheitsbestimmungen für elektrische Mess-, Steuer-, Regel- und
Laborgeräte – Teil 1: Allgemeine Anforderungen (DIN EN 610101:2011, VDE 411-1:2011)
Safety requirements for electrical equipment for measurement, control,
and laboratory use – Part 1: General requirements (IEC 61010-1:2010,
BS EN 61010-1:2010)
Règles de sécurité pour appareils électriques de mesurage, de
régulation et de laboratoire – Partie 1: Prescriptions générales (CEI
61010-1:2010, NF EN 61010:2011)
EN 61010-2-010:2003
Sicherheitsbestimmungen für elektrische Meß-, Steuer-, Regel- und
Laborgeräte – Teil 2-010: Besondere Anforderungen an Laborgeräte für
das Erhitzen von Stoffen (DIN EN 61010-2-010:2004)
Safety requirements for electrical equipment for measurement, control,
and laboratory use – Part 2-010: Particular requirements for laboratory
equipment for the heating of materials (IEC 61010-2-10:2005, BS EN
61010-2-10:2003)
Règles de sécurité pour appareils électriques de mesurage, de
régulation et de laboratoire – Partie 2-010 : Prescriptions particulières
pour appareils de laboratoire utilisés pour l’échauffement des matières
(CEI 61010-2-10:2003, NF EN 61010-2-10:2005)
EMV / EMC / CEM:
EN 61326-1:2006
+ Corr. 1:2008 + Corr. 2:2010
Elektrische Mess-, Steuer-, Regel- und Laborgeräte - EMVAnforderungen - Teil 1: Allgemeine Anforderungen (DIN EN 613261:2006 + Berichtigung 1:2008 + Berichtigung 2:2011)
Electrical equipment for measurement, control and laboratory use EMC requirements - Part 1: General requirements (IEC 61326-1:2005 +
Corr. 1:2008 + Corr. 2:2010, BS EN 61326-1:2006+ A1:2008)
Matériel électrique de mesure, de commande et de laboratoire Exigences relatives à la CEM - Partie 1: Exigences générales (CEI
61326-1:2005 + AC1:2008, NF EN 61326-1:2006 mod.)
EN 61326-2-2:2006
Elektrische Mess-, Steuer-, Regel- und Laborgeräte – EMVAnforderungen. Teil 2-2: Besondere Anforderungen - Prüfanordnung,
Betriebsbedingungen und Leistungsmerkmale für ortsveränderliche
Prüf-, Mess- und Überwachungsgeräte in NiederspannungsStromversorgungsnetzen. (DIN EN 61326-2-2:2006)
Electrical equipment for measurement, control and laboratory use –
EMC requirements. Part 2-2: Particular requirements - Test
configurations, operational conditions and performance criteria for
portable test, measuring and monitoring equipment used in low-voltage
distribution systems. (IEC 61326-2-2:2005, BS EN 61326-2-2:2006)
Matériel électrique de mesure, de commande et de laboratoire –
Exigences relatives à la CEM. Partie 2-2: Exigences particulières Configurations d’essai, conditions de fonctionnement et critères
d’aptitude à la fonction des matériels portatifs d’essai, de mesure et de
surveillance utilisés dans des systèmes de distribution basse tension.
(CEI 61326-2-2:2005 + AC1:2007, NF EN 61326-2-2:2006)
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D-78532 Tuttlingen, 20.07.2012
BINDER GmbH
P. M. Binder
B. Hofmann
Geschäftsführender Gesellschafter
Managing Director
Directeur général
Leiter F & E
Director R & D
Chef de service R&D
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Product registration
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Contents
EG – declaration of conformity ...................................................................................................................... 2
Product registration ....................................................................................................................................... 5
1.
SAFETY ................................................................................................................ 10
1.1
1.2
Legal considerations ......................................................................................................................... 10
Structure of the safety instructions .................................................................................................... 10
1.2.1
Signal word panel ................................................................................................................... 10
1.2.2
Safety alert symbol ................................................................................................................. 11
1.2.3
Pictograms .............................................................................................................................. 11
1.2.4
Word message panel structure ............................................................................................... 12
1.3 Localization / position of safety labels on the unit ............................................................................. 12
1.4 Type plate.......................................................................................................................................... 14
1.5 General safety instructions on installing and operating the CO2 incubator....................................... 15
1.6 Precautions when working with gases .............................................................................................. 16
1.7 Precautions when handling gas cylinders ......................................................................................... 18
1.8 Intended use ..................................................................................................................................... 18
2.
UNIT DESCRIPTION ............................................................................................ 19
2.1
2.2
2.3
2.4
Unit overview ..................................................................................................................................... 20
Instrument panel ............................................................................................................................... 20
Inner chamber ................................................................................................................................... 21
Control panel on the rear of the unit ................................................................................................. 23
3.
COMPLETENESS OF DELIVERY, TRANSPORTATION, STORAGE, AND
INSTALLATION.................................................................................................... 24
3.1
3.2
3.3
3.4
Unpacking, and checking equipment and completeness of delivery ................................................ 24
Guidelines for safe lifting and transportation..................................................................................... 24
Storage .............................................................................................................................................. 25
Location of installation and ambient conditions ................................................................................ 25
4.
INSTALLATION AND CONNECTIONS ............................................................... 29
4.1
4.2
4.3
Shelves.............................................................................................................................................. 29
Permadry™ water pan ...................................................................................................................... 29
Gas connections ............................................................................................................................... 30
4.3.1
Connection of the CO2 gas cylinder ........................................................................................ 31
4.3.2
Connection of the O2 gas cylinder (unit with O2 control)......................................................... 32
4.3.3
Connection of the N2 gas cylinder (unit with O2 control) ......................................................... 33
4.3.4
Connecting the gas hose to the unit rear (for CO2, O2, N2) .................................................... 34
4.3.5
Gas cylinder connection kits (option) ...................................................................................... 35
4.4 Electrical connection ......................................................................................................................... 36
4.5 Handling and aligning the multiple-divided inner glass door, gas proof (optional equipment) ........ 37
5.
START UP ............................................................................................................ 38
5.1
5.2
Equilibration time ............................................................................................................................... 38
Factory settings ................................................................................................................................. 38
6.
FUNCTIONAL OVERVIEW OF THE T4.12 CHAMBER CONTROLLER ............. 39
6.1
Menu structure .................................................................................................................................. 40
6.1.1
General menu ......................................................................................................................... 40
6.1.2
Quick menu ............................................................................................................................. 41
6.1.3
User menu .............................................................................................................................. 42
6.2 Operating modes ............................................................................................................................... 43
6.2.1
Activating the “control off” mode or change to “fixed value” operating mode ......................... 43
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Deactivating the O2 control and O2/N2 pressure alarms (unit with O2 control) .................................. 45
6.3.1
Required gas supply of the unit with O2 control ...................................................................... 45
6.3.2
Activating / deactivating the O2 control and O2 / N2 pressure alarms ..................................... 46
6.4 Humidity control of the Permadry™ system...................................................................................... 47
6.5 Turning on / off the interior socket voltage (with optional interior socket) ......................................... 48
6.6 Performance during and after power failure...................................................................................... 48
6.7 Information ........................................................................................................................................ 49
6.3
7.
SETPOINT ENTRY ............................................................................................... 50
7.1
7.2
7.3
7.4
Setting ranges ................................................................................................................................... 50
Note when setting high gas concentrations ...................................................................................... 50
Entering the setpoints via “quick menu” ............................................................................................ 52
Entering the setpoints via general menu........................................................................................... 54
8.
KEY LOCK ........................................................................................................... 56
8.1
8.2
8.3
Directly activating the key lock .......................................................................................................... 57
Automatic key lock ............................................................................................................................ 57
Changing the password for unlocking the key lock ........................................................................... 59
9.
GENERAL CONTROLLER SETTINGS ................................................................ 60
9.1
9.2
9.3
9.4
9.5
9.6
9.7
9.8
9.9
9.10
9.11
9.12
Setup wizard ..................................................................................................................................... 61
Date and time settings ...................................................................................................................... 61
Selecting the menu language of the T4.12 controller ....................................................................... 63
Setting display brightness ................................................................................................................. 63
Changing the temperature unit.......................................................................................................... 64
Defining the data recording rate ........................................................................................................ 64
Factory reset ..................................................................................................................................... 65
Network configuration ....................................................................................................................... 65
Display of the network configuration ................................................................................................. 69
MAC Address .................................................................................................................................... 69
RS 422 address (with optional RS 422 interface) ............................................................................. 70
Display and entry of the device configuration – for service purpose ................................................ 71
10. DATA TRANSFER VIA USB INTERFACE........................................................... 72
10.1 Exporting data to USB drive .............................................................................................................. 72
10.2 Importing data from USB drive .......................................................................................................... 73
11. NOTIFICATIONS AND ALARMS ......................................................................... 74
11.1 Notifications overview ....................................................................................................................... 74
11.2 Alarm messages overview ................................................................................................................ 74
11.3 Alarm status ...................................................................................................................................... 76
11.4 Confirming an active “set” alarm ....................................................................................................... 77
11.5 Alarm configuration and overview ..................................................................................................... 78
11.5.1 List of active alarms ................................................................................................................ 78
11.5.2 History – list of all alarms ........................................................................................................ 79
11.5.3 Activating, deactivating, and testing the alarm buzzer ........................................................... 80
11.5.4 Activating / deactivating all alarm functions ............................................................................ 80
11.5.5 Setting the delay time after opening the door ......................................................................... 81
11.5.6 Tolerance ranges and alarm delay times................................................................................ 81
11.6 Zero-voltage relay alarm output ........................................................................................................ 86
12. EVENT LIST ......................................................................................................... 87
13. GRAPHICAL DISPLAY OF THE MEASURED VALUES ..................................... 88
13.1 Setting the sampling rate .................................................................................................................. 88
13.2 Defining the display range................................................................................................................. 89
13.3 Selecting the parameters .................................................................................................................. 90
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14. TEMPERATURE SAFETY DEVICES ................................................................... 91
14.1 Overtemperature protective device (class 1) .................................................................................... 91
14.2 Overtemperature safety controller (temperature safety device class 3.1) ........................................ 91
14.2.1 Safety controller modes .......................................................................................................... 91
14.2.2 Setting the safety controller .................................................................................................... 92
15. OPTIONS .............................................................................................................. 95
15.1 Communication software APT-COM™ 3 DataControlSystem (option) ............................................ 95
15.2 RS 422 interface (option) .................................................................................................................. 95
15.3 Silicone access ports 30 mm / 1.18 in, closable from both sides with silicon plugs (option) ............ 95
15.4 Interior socket 230V (option) ............................................................................................................. 96
15.5 Analog outputs for temperature and CO2 (option) ............................................................................ 98
15.6 Access port for extra-low voltage (option)......................................................................................... 98
15.7 BINDER Gas Supply Service – External bottle changer for CO2, N2 or O2 (option) ......................... 99
15.8 Stands ............................................................................................................................................. 100
15.8.1 Stacking stand (option) ......................................................................................................... 100
15.8.2 Stacking adapter for direct thermal decoupled stacking (option) ......................................... 100
15.8.3 Base on castors (option) ....................................................................................................... 100
16. REFERENCE MEASUREMENTS ...................................................................... 101
16.1 CO2 reference measuring ............................................................................................................... 101
16.1.1 Measuring CO2 concentration indirectly via the pH of the cell medium ................................ 101
16.1.2 Measuring CO2 directly via chemical indicator tubes............................................................ 102
16.1.3 Measuring CO2 directly with an electronic infrared measuring device ................................. 103
16.2 Temperature reference measurement ............................................................................................ 103
17. AVOIDING MICROBIAL CONTAMINATION...................................................... 103
17.1
17.2
17.3
17.4
17.5
Cells and media .............................................................................................................................. 103
Laboratory conditions / equipment around the incubator ................................................................ 103
Working and behavior in the lab ..................................................................................................... 104
Chamber design and equipment of the CO2 incubator ................................................................... 104
Handling the CO2 incubator ............................................................................................................ 105
18. CLEANING, DECONTAMINATION / DISINFECTION, AND STERILIZATION .. 107
18.1 Cleaning .......................................................................................................................................... 107
18.2 Decontamination / chemical disinfection of the CO2 incubator ....................................................... 108
18.3 Hot-air sterilization at 180 °C / 356 °F ............................................................................................. 110
18.3.1 Overview ............................................................................................................................... 110
18.3.2 Performing a hot-air sterilization ........................................................................................... 111
18.3.3 Aborting the hot-air sterilization prematurely ........................................................................ 113
18.3.4 End of the sterilization cycle ................................................................................................. 115
19. MAINTENANCE AND SERVICE ........................................................................ 116
19.1
19.2
19.3
19.4
19.5
Maintenance intervals, service ........................................................................................................ 116
Checking the air jacket heating fan ................................................................................................. 116
Checking the humidity system fan .................................................................................................. 117
Gas inlet fine filter ........................................................................................................................... 117
Sending the unit back to BINDER GmbH ....................................................................................... 117
20. DISPOSAL.......................................................................................................... 118
20.1 Disposal of the transport packing .................................................................................................... 118
20.1.1 Outer unit packing ................................................................................................................. 118
1.1.1
Packing inside the unit and equipment ................................................................................. 118
20.2 Decommissioning ............................................................................................................................ 119
20.3 Disposal of the unit in the Federal Republic of Germany ............................................................... 119
20.4 Disposal of the unit in the member states of the EC except for the Federal Republic of Germany 120
20.5 Disposal of the unit in non-member states of the EC ..................................................................... 121
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21. TROUBLESHOOTING ....................................................................................... 122
21.1
21.2
21.3
21.4
21.5
21.6
21.7
Heating ............................................................................................................................................ 122
Gas cylinder pressure too low ......................................................................................................... 123
Gas concentration ........................................................................................................................... 125
Sterilization ...................................................................................................................................... 127
Humidity .......................................................................................................................................... 127
Controller ......................................................................................................................................... 129
Open door ....................................................................................................................................... 129
22. TECHNICAL DESCRIPTION .............................................................................. 130
22.1
22.2
22.3
22.4
22.5
22.6
22.7
22.8
22.9
22.10
22.11
Factory calibration and adjustment ................................................................................................. 130
Over current protection ................................................................................................................... 130
Definition of usable volume ............................................................................................................. 130
CB technical data ............................................................................................................................ 131
Equipment and Options ................................................................................................................... 133
Spare parts and accessories .......................................................................................................... 134
Important conversion data for non-SI units ..................................................................................... 135
Conversion table for gas inlet pressures, bar – psi ......................................................................... 135
Dimensions CB 60 .......................................................................................................................... 136
Dimensions CB 160 ........................................................................................................................ 137
Dimensions CB 220 ........................................................................................................................ 138
23. CONTAMINATION CLEARANCE CERTIFICATE ............................................. 139
23.1 For units located outside North America and Central America ....................................................... 139
23.2 For units in North America and Central America ............................................................................ 142
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Dear customer,
For the correct operation of the CO2 incubator CB, it is important that you read this operating manual
completely and carefully and observe all instructions as indicated. Failure to read, understand and follow
the instructions may result in personal injury. It can also lead to damage to the unit and/or poor equipment
performance
1.
Safety
This operating manual is part of the components of delivery. Always keep it handy for reference. The
device should only be operated by laboratory personnel especially trained for this purpose and familiar
with all precautionary measures required for working in a laboratory. To avoid injuries and damage
observe the safety instructions of the operating manual.
WARNING
Failure to observe the safety instructions.
Serious injuries and unit damage.
Observe the safety instructions in this operating manual.
Carefully read the complete operating instructions of the CO2 incubator CB.
1.1
Legal considerations
This operating manual is for informational purposes only. It contains information for installing, start-up,
operation and maintenance of the product. Note: the contents and the product described are subject to
change without notice.
Understanding and observing the instructions in this operating manual are prerequisites for hazard-free
use and safety during operation and maintenance. In no event shall BINDER be held liable for any
damages, direct or incidental arising out of or related to the use of this manual.
This operating manual cannot cover all conceivable applications. If you would like additional information,
or if special problems arise that are not sufficiently addressed in this manual, please ask your dealer or
contact us directly by phone at the number located on page one of this manual
Furthermore, we emphasize that the contents of this operating manual are not part of an earlier or
existing agreement, description, or legal relationship, nor do they modify such a relationship. All
obligations on the part of BINDER derive from the respective purchase contract, which also contains the
entire and exclusively valid statement of warranty administration. The statements in this manual neither
augment nor restrict the contractual warranty provisions.
1.2
Structure of the safety instructions
In this operating manual, the following safety definitions and symbols indicate dangerous situations
following the harmonization of ISO 3864-2 and ANSI Z535.6.
1.2.1
Signal word panel
Depending on the probability of serious consequences, potential dangers are identified with a signal
word, the corresponding safety color, and if appropriate, the safety alert symbol.
DANGER
Indicates an imminently hazardous situation that, if not avoided, will result in death or serious
(irreversible) injury.
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WARNING
Indicates a potentially hazardous situation which, if not avoided, could result in death or serious
(irreversible) injury.
CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor
(reversible) injury.
CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in damage to the product
and/or its functions or of a property in its proximity.
1.2.2
Safety alert symbol
Use of the safety alert symbol indicates a risk of injury.
Observe all measures that are marked with the safety alert symbol in order to avoid death or
injury.
1.2.3
Pictograms
Warning signs
Electrical hazard
Hot surface
Explosive atmosphere
Stability hazard
Lifting hazard
Gas cylinders
Suffocation hazard
CO2 suffocation and
poisoning hazard
Explosive substances
Fire promoting agents
Harmful substances
Risk of corrosion and /
or chemical burns
Biohazard
Pollution Hazard
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Mandatory action signs
Mandatory regulation
Read operating
instructions
Disconnect the power
plug
Environment protection
Prohibition signs
Wear protective gloves
Wear safety goggles
Do NOT touch
Do NOT spray with
water
Do NOT climb
Lift with several persons
Information to be observed in order to ensure optimum function of the product.
1.2.4
Word message panel structure
Type / cause of hazard.
Possible consequences.
Instruction how to avoid the hazard: prohibition
Instruction how to avoid the hazard: mandatory action
Observe all other notes and information not necessarily emphasized in the same way, in order to avoid
disruptions that could result in direct or indirect injury or property damage.
1.3
Localization / position of safety labels on the unit
The following labels are located on the unit:
Pictograms (Warning signs)
Service label
Hot surface
Risk of injury
• on the outer door: CB-UL only
• above the access ports (option)
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CO2 -Incubator CB
CO2 -Incubator CB-UL
Figure 1: Position of labels on the unit
Keep safety labels complete and legible.
Replace safety labels that are no longer legible. Contact BINDER service for these replacements.
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1.4
Type plate
Position of type plate: left unit side (seen from front), at the bottom in the middle
Nominal temperature 187 °C
369°F
Enclosure protection IP 20
Temp. safety device
DIN 12880
Class
3.1
Art. No.
9040-0092
Project No.
1,30 kW
200-240 V 1 N ~
5,7 A
50/60 Hz
US PATS 4585923 / 5222612 / 5309981
5405194 / 5601143 / 5773287 / 6079403
D 78532 Tuttlingen / Germany
Tel. + 49 (0) 7462/ 2005-0
Internet: www.binder-world.com
CB 160 Serial No. 00-00000
Made in Germany
Figure 2: Type plate (example of CB 160 regular unit)
Indications of the type plate
BINDER
CB 160
Serial No.
00-00000
Nominal temperature 1 °C
369 °F
Enclosure protection IP 20
Temp. safety device
DIN 12880
Class
3.1
Art. No.
9040-0092
Project No.
--1,30 kW
200-240 V 1 N ~
5,7 A
50/60 Hz
Information
Manufacturer: BINDER GmbH
Model CB 160
Serial No. 00-00000
Symbol on the type plate
Information
Nominal temperature
IP type of protection 20 acc. to EN 60529
Temperature safety device acc. to standard DIN 12880
Temperature safety device, class 3.1
Art. No. 9040-0092
(Special application acc. to project no.)
Nominal power 1,30 kW
Nominal voltage 200-240 V ± 10%, single-phase unit
Nominal current 5,7 Amp
Power frequency 50/60 Hz
CE conformity marking
Electrical and electronic equipment manufactured / placed on
the market in the EC after 13 August 2005 and to be disposed of
in separate collection according to directive 2002/96/EC on
waste electrical and electronic equipment (WEEE).
The equipment is certified in the GOST R certification system of
GOSTSTANDARD Russia.
®
(CB-UL only)
LABORATORY EQUIPMENT
43KM
CB (E6) 09/2013
The equipment is certified by Underwriters Laboratories Inc.
nd
according to standards CAN/CSA-C22.2 No. 61010-1, 2
Edition, 2004-07 (Electrical Equipment for Measurement,
Control, and Laboratory Use; Part 1: General Requirements); UL
nd
61010-1, 2 Edition, 2005-07-22 (Electrical Equipment for
Measurement, Control, and Laboratory Use; Part 1: General
rd
Requirements); IEC 61010-1:2010, 3 Edition and IEC 61010-210:2003 (Particular Requirements for Laboratory Equipment for
the heating of materials).
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1.5
General safety instructions on installing and operating the CO2 incubator
With regard to operating the CO2 incubator CB and to the installation location, please observe the
guideline BGI/GUV-I 850-0 on safe working in laboratories (formerly BGR/GUV-R 120 or ZH 1/119
laboratory guidelines issued by the employers’ liability insurance association) (for Germany).
BINDER GmbH is only responsible for the safety features of the unit provided skilled electricians or
qualified personnel authorized by BINDER perform all maintenance and repair, and if components
relating to chamber safety are replaced in the event of failure with original spare parts.
To operate the unit, use only original BINDER accessories or accessories from third-party suppliers
authorized by BINDER. The user is responsible for any risk caused by using unauthorized accessories.
CAUTION
Danger of overheating.
Damage to the unit.
∅ Do NOT install the unit in unventilated recesses.
Ensure sufficient ventilation for dispersal of the heat.
Do not operate the CO2 incubator CB in hazardous locations.
DANGER
Explosion hazard.
Danger of death.
∅ Do NOT operate the unit in potentially explosive areas.
KEEP explosive dust or air-solvent mixtures AWAY from the unit.
The CO2 incubator CB does not dispose of any measures of explosion protection.
DANGER
Explosion hazard.
Danger of death.
∅ Do NOT introduce any substance into the CO2 incubator which is combustible or
explosive at working temperature.
∅ NO explosive dust or air-solvent mixture in the inner chamber.
Any solvent contained in the charging material must not be explosive or inflammable. I.e., irrespective of
the solvent concentration in the steam room, NO explosive mixture with air must form. The temperature
inside the chamber must lie below the flash point or below the sublimation point of the charging material.
Familiarize yourself with the physical and chemical properties of the charging material, as well as the
contained moisture constituent and its behavior with the addition of heat energy.
Familiarize yourself with any potential health risks caused by the charging material, the contained
moisture constituent or by reaction products which may arise during the temperature process. Take
adequate measures to exclude such risks prior to putting the CO2 incubator into operation.
DANGER
Electrical hazard.
Danger of death.
∅ The unit must NOT become wet during operation or maintenance.
The CO2 incubators were produced in accordance with VDE regulations and were routinely tested in
accordance to VDE 0411-1 (IEC 61010-1).
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Page 16
CAUTION
The glass doors and the inner chamber will become hot during operation.
Danger of burning.
∅ Do NOT touch the glass doors, the inner surfaces or the charging material during
operation.
WARNING
Stability hazard.
Danger of injury.
Damage to the unit and the charging material.
Housing cover breakaway.
∅ Do NOT climb on the lower housing cover.
∅ Do NOT load the lower housing cover with heavy objects while the unit door is open.
1.6
Precautions when working with gases
Notes on handling carbon dioxide (CO2)
Carbon dioxide (CO2) in high concentrations is hazardous to health. It is colorless and almost odorless
and therefore practically imperceptible. Vent out any CO2 gas that may escape via good room ventilation
or a suitable connection to an exhaust system. We recommend installing a CO2 warning system
WARNING
High concentration of CO2 (> 4 Vol.-%).
Risk of death by suffocation.
Danger of poisoning.
∅ Do NOT set up units in non-ventilated recesses.
Ensure technical ventilation measures.
Observe the relevant regulations for handling CO2.
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Page 17
Unit with O2 control: Notes on handling oxygen (O2)
Oxygen (O2) is colorless and almost odorless and therefore practically imperceptible. It promotes burns,
which can proceed explosively. There is a fire hazard for flammable oxygenated materials, e.g. clothes
and hair. O2 is heavier than air and may accumulate in low-lying areas.
WARNING
High concentration of O2 (> 21 % O2).
Fire and explosion hazard through contact of combustible materials with O2.
Risk of burns and other injuries.
∅ Do NOT set up units in non-ventilated recesses.
Ensure technical ventilation measures.
Observe the relevant regulations for handling O2.
Take appropriate measures to prevent oxygen enrichment and fire and explosion hazards in areas where
oxygen enrichment is possible.
General information for safe handling of oxygen:
• Make sure training of personnel on hazards of oxygen enrichment and necessary safety
measures.
• Make sure adequate labeling of all oxygen equipment and facilities.
• Make sure gas tightness of all gas connections by checking them for leaks (e.g. with leak
spray or diluted soap solution).
• Close the main valve of the source of oxygen after work when not using the chamber.
• Never lubricate O2 equipment with oil or fat. Use only materials and spare parts which are
approved for use with oxygen.
• Regularly inspect fire extinguishers for proper condition.
• Set up emergency showers where oxygen enrichment is possible.
• Strictest smoking ban and no ignition sources in areas where oxygen enrichment is
possible.
• Make sure good ventilation of areas where oxygen enrichment is possible (location of the
chamber and/or O2 cylinders.
• Persons who may have been in a possibly oxygen-enriched atmosphere must keep away
from ignition sources (flames, cigarettes, etc.) and ventilate their clothes at least 15
minutes.
• Always keep emergency routes free.
Unit with O2 control: Notes on handling nitrogen (N2)
Nitrogen (N2) in high concentrations is hazardous to health. It is colorless and almost odorless and
therefore practically imperceptible. Any N2 gas that may escape must be safely led out via good room
ventilation or a suitable connection to an exhaust system.
WARNING
High concentration of N2.
Risk of death by suffocation.
∅ Do NOT set up units in non-ventilated recesses.
Ensure technical ventilation measures.
Observe the relevant regulations for handling N2.
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1.7
Precautions when handling gas cylinders
General information for safe handling of gas cylinders:
• Store and use gas cylinders only in well-ventilated locations.
• Open the gas cylinder valve slowly to avoid pressure surges.
• Secure gas cylinders during storage and use against falling (chaining).
• Transport gas cylinders with a cylinder cart, do not carry, roll, or throw them.
• Always close the valve even with apparently empty cylinders; screw on the cap when not in
use. Return gas cylinders with the valve closed.
• Do not open gas cylinders by force. Mark them when damaged.
• Protect gas cylinders against fire, e.g. do not store together with flammable liquids.
• Observe relevant regulations for dealing with gas cylinders.
Secure the gas cylinders against falling and other mechanical damage.
WARNING
Safety valve tearing off.
Sudden release of the stored pressure energy.
Risk of injury.
Secure gas cylinders against falling (chaining).
Transport gas cylinders with a cylinder cart.
The valve of the gas cylinder always must be closed before screwing on or unscrewing the gas hose.
WARNING
Opening the cylinder valve when the cylinder is not connected.
Sudden release of the stored pressure energy.
Risk of injury.
Close the gas cylinder valve before connecting or removing the gas hose.
After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or
diluted soap solution).
1.8
Intended use
Series CB incubators are suitable for the cultivation of mammal cells under typical conditions of approx.
37 °C / 98.6°F. The incubator permits setting defined pH conditions by common NaHCO3 buffer systems
of commercial cell media by keeping an exact CO2 atmosphere inside. CB incubators guarantee high
humidity inside to avoid osmolarity increasing caused by the evaporation of the cell media.
With the unit with O2 control, a variable oxygen atmosphere can additionally influence the growth of the
cells.
Following the instructions in this operating manual and conducting regular maintenance work
(chap. 21.1) are part of the intended use.
Other applications are not approved.
WARNING: If customer should use a BINDER chamber running in non-supervised
continuous operation, we strongly recommend in case of inclusion of irrecoverable specimen
or samples to split such specimen or samples and store them in at least two chambers, if this
is feasible.
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Page 19
2.
Unit description
The CO2 incubators CB are equipped with a multifunctional microprocessor display controller for
temperature, CO2, and O2 (unit with O2 control) levels and a digital display accurate to one-tenth of a
degree resp. 0.1 vol.-%
The inner chamber, the pre-heating chamber and the inside of the doors are all made of stainless steel
(material no. 1.4301 (V2A) in Germany). The inner surfaces are smooth and therefore easy to clean. The
inner chamber is deep-drawn from one piece, polished (suitable for pharmaceutical applications) and has
no welds or inaccessible corners. The hinges and the seal of the inner glass door are glued from the
outside to aid cleaning of the inner chamber. When operating the chamber at high temperatures
(sterilization), the impact of the oxygen in the air may cause discoloration of the metallic surfaces
(yellowish-brown or blue) by natural oxidation processes. These colorations are harmless and will in no
way impair the function or quality of the unit.
The perforated shelves are also made of stainless steel. You can insert a maximum of 3 (CB 60), 6 (CB
160), resp. 8 (CB 220) shelves.
The housing is RAL 7035 powder-coated. All corners and edges are also completely coated.
The heating system of the CO2 incubator permits hot-air auto-sterilization at a setpoint of 187.5 °C /
369.5°F. Thus, a temperature of 180 °C / 356°F is maintained for at least 30 minutes on all internal
surfaces, resulting in sterilization of the entire inner chamber. Therefore, this procedure meets all
international guidelines regarding hot air sterilization, e.g. AAMI ST63, DIN 58947, European
Pharmacopoeia.
Thanks to the standard safety device (class 3.1 according to DIN 12880), the set temperature is
maintained in case of failure.
The gas enters the chamber via a fine filter (aseptic filter) with a high filtration efficiency that also filters
the smallest particles.
A highly precise, drift-free CO2 infrared measuring system in combination with the permanent mixture of
CO2 gas through a special proprietary gas mixing head developed by BINDER allows precise and
constant CO2 concentrations for long periods. This creates optimum growth conditions for cultures.
The CO2 incubator is also available with O2 control in addition to CO2 control.
CO2 and O2 sensors
Fast reaction times, maximum accuracy and selectivity characterize the CO2 measuring procedure of
the CB incubator series. The accuracy of the CO2 measuring system is based on a double-beam
infrared measuring cell with NDIR (non-dispersive infrared) sensor, which continuously regulates to a
reference value. Therefore, disturbance variables and aging phenomena in the measuring system are
almost completely eliminated, so that this measuring system, in contrast to other measuring
procedures, remains practically drift-free between calibrations and is entirely selective for CO2 and
humidity. The sensor is built into the incubator and can be sterilized.
The O2 sensor is a semiconductor gas sensor with ZrO2 ceramic.
The accuracy of the indicated values of CO2 and O2 (unit with O2 control) depends on the ambient air
pressure (approx. 0.08 vol.-% per 10 mbar / 0.15 psi). To compensate for this effect in the CO2
measurement, the controller measures the ambient air pressure and automatically includes it in the
calculation.
The CO2 incubators CB are equipped with an Ethernet interface for computer communication, e.g. with
the communication software APT-COM™ 3 DataControlSystem (option, chap. 17.1). For further options,
see chap. 24.4.
Temperature range: 7 °C / 12.6 °F above ambient temperature up to +60 °C / 140 °F
CO2 range: 0 vol.-% up to 20 vol.-%)
O2 range (unit with O2 control): 0.2 vol.-% up to 95 vol.-%
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Page 20
2.1
Unit overview
Instrument panel with
microprocessor controller
T4.12 and USB interface
Door handle
Unit door
Main power switch
Figure 3: CO2 incubator CB (example: model CB 160)
2.2
Instrument panel
5,7" controller display
Context-sensitive buttons
USB interface
Operating button
Pilot lamp: ready for operation
Figure 4: Instrument panel with microprocessor controller T4.12 and USB interface
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Page 21
2.3
Inner chamber
(A)
(B)
(C)
(D)
(E)
(G)
(H)
FRONT
(I)
(J)
(K)
(L)
Figure 5: CB 160 with O2 control and options
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Page 22
(B)
(C)
(D)
(D2)
(F)
(G)
(E)
Figure 6: CB 160 / CB 220 with O2 control and options
(A)
Instrument panel with microprocessor controller T4.12, indicating temperature and CO2 as well as
O2 (unit with O2 control)”
(B)
Connection socket for extra-low voltage supply (option, chap. 17.6)
(C)
CO2 and humidity sensor
(D)
Gas mixing head CO2
(D2) Additional gas mixing head O2/ N2 (unit with O2 control)
(E)
Pt 100 temperature probe
(F)
O2 sensor (unit with O2 control)
(G)
Internal socket 230V (max. 3 A) (option, chap. 17.4)
(H)
Perforated shelves, made of stainless steel
(I)
Measuring access port
(J)
Inner glass doors
(K)
Permadry™ water pans
(L)
Lower housing cover
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2.4
Control panel on the rear of the unit
(1)
(2)
(3)
(7)
(8)
(9)
(6)
(4)
(5)
(10)
Figure 7: Rear control panel CB with O2 control and options
(1)
Miniature fuse
(2)
External socket for extra-low voltage supply (option for CB 160 / CB 220, chap. 17.6)
(3)
DIN socket for analog outputs 4-20 mA (option, chap. 17.5)
(4)
Quick acting closure socket for CO2
(5)
Quick acting closure socket for O2 (unit with O2 control)
(6)
Quick acting closure socket for N2 (unit with O2 control)
(7)
Socket for IEC connector plug for power cable
(8)
Ethernet interface for computer communication
(9)
DIN-socket for zero-voltage relay alarm outputs
(10)
RS 422 interface for computer communication (option)
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Page 24
3.
Completeness
installation
of
delivery,
transportation,
storage,
3.1
Unpacking, and checking equipment and completeness of delivery
and
After unpacking, please check the unit and its optional accessories, if any, based on the delivery receipt
for completeness and for transportation damage. Inform the carrier immediately if transportation damage
has occurred.
The final tests of the manufacturer may have caused traces of the shelves on the inner surfaces. This has
no impact on the function and performance of the unit.
Please remove any transportation protection devices and adhesives in/on the unit and on the doors and
remove the operating manuals and accessory equipment.
Remove any protective lamination sheet on the inner metal surfaces prior to commissioning.
CAUTION
Sliding or tilting of the unit.
Damage to the unit.
Risk of injury by lifting heavy loads.
Do NOT lift or transport the unit using the door handle, the door or the lower housing.
Lift the unit from the pallet at the four lower corners with the aid of four people.
If you need to return the unit, please use the original packing and observe the guidelines for safe lifting
and transportation (chap. 3.2).
For disposal of the transport packing, see chap. 22.1.
Note on second-hand units (Ex-Demo-Units):
Second-hand units are units that were used for a short time for tests or exhibitions. They are thoroughly
tested before resale. BINDER ensures that the chamber is technically sound and will work flawlessly.
Second-hand units are marked with a sticker on the unit door. Please remove the sticker before
commissioning the unit.
3.2
Guidelines for safe lifting and transportation
After operation, please observe the guidelines for temporary decommissioning (chap. 22.2). Empty the
Permadry™ water pan before moving the incubator. In case of any spilling of the contents, shut down the
incubator and dry it out carefully and completely.
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CAUTION
Sliding or tilting of the unit.
Damage to the unit.
Risk of injury by lifting heavy loads.
Transport the unit in its original packaging only.
For moving or shipping, secure the unit with transport straps.
∅ Do NOT lift or transport the unit using the door handle, the door or the lower housing.
Lift the unit at the four lower corners with the aid of 4 people and place it on a rolling
pallet.
Move the unit to the desired location and lift it from the rolling pallet with the aid of four
people.
• Permissible ambient temperature range during transport: 10 °C / 14 °F to +60 °C / 140 °F.
You can order transport packing for moving or shipping purposes from BINDER service.
3.3
Storage
Intermediate storage of the unit is possible in a closed and dry room. Observe the guidelines for
temporary decommissioning (chap. 22.2).
• Permissible ambient temperature range during storage: -10 °C / 14 °F to +60 °C / 140 °F.
• Permissible ambient humidity: max. 70 % r.H., non-condensing
When after storage in a cold location you transfer the unit to its warmer installation site, condensation
may form. Before start-up, wait at least one hour until the chamber has attained ambient temperature and
is completely dry.
3.4
Location of installation and ambient conditions
Notes on the location of installation
Set up the CO2 incubator on a flat, even surface, free from vibration and in a well-ventilated, dry location.
The chambers are designed for setting up inside a building (indoor use).
Freestanding CB incubators are suitable for installation on tables or on the optionally available stand
(height 200 mm / 0.5 ft). Note: The site of installation must be capable of supporting the unit’s weight (see
technical data, chap.24.3).
Align the unit using a spirit level to ensure even covering of the cell-cultures with the medium. For this
purpose, manually adjust the four incubator feet.
CO2 incubators can be stacked on top of each other (two units maximum). For safe stacking that is easy
to maintain, use the original BINDER stacking stand (chap. 17.9.1) or the stacking adapter (chap. 17.9.2).
CAUTION
Sliding of stacked unit.
Damage to the units.
When stacking, use rubber pads (Art. No. 8012-0376) for the feet of the upper
incubator.
To completely separate the unit from the power supply, you must disconnect the power plug. Install the
unit in a way that the power plug is easily accessible and can be easily pulled in case of danger.
In order to avoid contamination, never place the unit directly on the floor.
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