Hangzhou Allsheng Instruments Co
MS-100 Thermo Shaker Operations Manual Ver 2.0
Operations Manual
19 Pages

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Operations Manual Version 2.0
MS-100 Thermo Shaker
Hangzhou Allsheng Instruments Co., Ltd
Foreword Thank you for purchasing our Products: MS-100 Thermo Shaker. This Manual for users contains function and operation of the Instrument. In order to use the instrument properly, please read this manual carefully before using the Instrument. Keep it for later use when you meet with difficulties.
Opening Check Please check the Instrument and Appendix with the packing list when you first open the instrument packing case. If you find there is something wrong with the Instrument and the Appendix, do contact the vendor or the producer.
Hangzhou Allsheng Instruments Co., Ltd. Address: Building 1&2, Zheheng Science Park, Zhuantang Town, Xihu District, Hangzhou, Zhejiang 310024, China Telephone: +86-571-88802738,88948289 Fax: +86-571-87205673 Post code: 310024 Website: www.allsheng.com Email: [email protected]
File No.:AS02SM Version No.:V2.0, Dec. 2019
Safety Warnings and Guidelines 1. Important operation information of the security: Before the users’ operation, they should have a perfect conception of how to use the Instrument. Therefore, read this Manual carefully before using it. Operation before reading the Manual is forbidden. Read the guidelines and directions below and carry out the countermeasure according to them.
2. Security: The operation, maintenance and repair of the Instrument should comply with the basic guidelines and the remarked warning below. If you don’t comply with them, it will have effect on the scheduled using life of the Instrument and the protection provided. This product is a normal and an indoor Instrument.
Read the Manual carefully before operation, The expert of wiring Read the Manual carefully before operation, The expert of wiring Read the Manual carefully before operation, The expert of wiring Read the Manual carefully before operation, The expert of wiring Read the Manual carefully before operation, the expert of equipment can operate this Instrument. equipment can operate this Instrument. equipment can operate this Instrument. equipment can operate this Instrument. wiring equipment can operate this Instrument. The operator should not open or repair the Instrument by himself, which will result in losing the qualification of repair guarantee or occurring accident. If there is some wrong with the Instrument, the company will repair it. A.C. power’s grounding should be reliable to safeguard against an electric shock. The 3-pin plug supplied with thermo-shaker’s power cable is a safety device that should be matched with a suitable grounded socket. During the normal operation, the temperature of metal block will be very high. There will be scald or boiling of the liquid. Therefore strictly prohibit any part of the body to touch the Instrument from scald.
Close the test tube lids before put the test tubes into the block. Liquids maybe spill in the block or onto the device if tube lids opened, that will damage the block or the device.
Before power on, guarantee the voltage used should be accordant to the voltage needed, and the rated load of electrical outlet should not lower than the demand. If the electric line is damaged, you should replace it with the same type. You should assure there’s nothing on the electric line and you should not put the electric line in the ambulatory place. Hold the jack when you pull out the electric line, and don’t pull the electric line. The Instrument should be put in the place of low temperature, little dust, no water and no sun or strong lamp. What’s more, the place should be good aeration, no corrosively gas or strong disturbing magnetic field, far away from central heating, camp stove and other hot resource. Don't put the Instrument in wet and dusty place. The vent on the Instrument is designed for aeration. Don’t wall up or cover the vent in order to keep from high temperature. If you use the more than one Instrument the same time, the distance between them should be more than 100cm. Mains switch is on the rear of the device, push “I” to power on the device, and push “O” to power off the device.
Power off when you finish your work. Pull off the connector plug when there’s long time no use of the Instrument and cover it with a cloth or plastic paper to prevent from dust. Pull the connector plug from the jack at once in the following case, and contact the vendor: There is some liquid flowing into the Instrument; Drenched or fire burned. Abnormal operation: such as abnormal sound or smell. Instrument dropping or outer shell damaged. The function has obviously changed.
3. The maintenance of Instrument The well in the block should be cleaned by the cloth stained with alcohol to assure good heat translation between the block and the test tube and no pollution. If there are smutches on the Instrument, clean them with cloth. Power off when cleaning the Instrument. When cleaning the well, don’t drop the cleaning liquid in the well. Corrosive cleaning liquid is strongly prohibited.
Contents
Chapter 1 Introduction---------------------------------------------------------- -----1 Chapter 2 Specifications-------------------------------------------------------- ----2 1 The normal operating condition--------------------------------------------2 2 The basic parameters and performance--------------------------------2 Chapter 3 Preparations-------------------------------------------------------------3 1 Structure Description----------------------------------------------------------3 2 Keyboard and Display panel-------------------------------------------------4 3 Key Functions--------------------------------------------------------------------5 Chapter 4 Operation Guide--------------------------------------------------------6 1 Single Temperature, Speed and Time setting--------------------------6 2 How to Shut Off the Temperature Speed and Timing--------------- 7 3 Short Mix---------------------------------------------------------------------------7 4 Temperature Calibration-------------------------------------------------------8 5 Exchange of Block-------------------------------------------------------------11 Chapter 5 Failure analysis and troubleshooting--------------------------12 Annex 1 Wiring Diagram of MS-100 Thermo Shaker---------------------13
MS-100 Thermo Shaker Operations Manual
Chapter 1 Introduction
Chapter 1 Introduction The Thermo shaker for Micro-tubes is an ideal instrument for intensive mixing of samples in the regulated temperature conditions. Mixing and heating modes can be used both simultaneously and independently i.e., the device can work as shaker and as a thermostat. The main body of the Mixing Block can be used with different kinds of blocks. MS-100 is applicable for DNA analysis, extraction of lipids and other cell components, DNA library creation, PCR amplification, pre-denaturation in electrophoresis, serum solidification etc.
Features of this product as follows: 1. As it is equipped with various optional mixing blocks, the instrument can adapt to different tubes & wells to cope with experimental needs. It is easy to replace the metal blocks and is very simple to clean and sterilize. Customized blocks are available to suit your specific demands 2. LCD display. It Easy to setup and use 3. Simultaneous display of set and actual time, temperature and speed 4. Over heating protection device ensures safety & reliability 5. Temperature can be calibrated to meet user's needs 6. Beep-signal / Stop after program completion 7. Can be produced for OEM customers
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MS-100 Thermo Shaker Operations Manual
Chapter 2 Specifications
Chapter 2 Specifications 1. The normal operating condition Ambient temperature: 5C ~35C The relative humidity: ≤70%
2. The basic parameters and performance Model
MS-100
Parameter Mixing rate
200~1500 rpm
Orbit
2mm
Temperature setting range
5℃~100℃
Timing range
1min~ 99h59min
Accuracy of the temperature
≤0.5℃
Heating time(RT25℃ )
Standard Block
Power Supply Fuse
≤12min (From 25℃ TO 100℃) A-BLOCK: 96x0.2 ml G-BLOCK:12x15ml Falcon B-BLOCK: 54 x 0.5ml H-BLOCK: 6x50ml Falcon C-BLOCK: 35 x 1.5ml I-BLOCK: 103X67X30mm Bath Block D-BLOCK: 35 x 2.0ml J-BLOCK:96x0.2ml Micro-plate E-BLOCK: 20 x 1.5ml + 15 x 0.5ml K-BLOCK:24x5ml Tube F-BLOCK:24x ≤Ф12mm Tube L-BLOCK:96-Deep Well Plate
AC100-120V~
AC200-240V~
50-60Hz
50-60Hz
250V
4.0A Ф5×20
250V
3.0A Ф5×20
Dimension (mm)
300(D)×220(W)×195(H)
Net weight (kg)
7.0
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MS-100 Thermo Shaker Operations Manual
Chapter 3 Preparations
Chapter 3 Preparations This chapter is introduces Thermo Shaker’s mechanical structure, the keyboard and each key’s functions and some preparations before power-on. You should be familiar with this chapter before the Thermo Shaker is first operated。
1. Structure Description
Standard Block
Keyboard
Power Switch
Power connector
Fuse
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MS-100 Thermo Shaker Operations Manual
Chapter 3 Preparations
2. Keyboard and Display panel
LCD
Display Panel:
Section in run
Current temperature
S1 100.0 S1 100.0 Setting Section
Current speed
1200 1200
Setting temperature
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Remnant time
10:30 15:00
Setting speed
Setting time
MS-100 Thermo Shaker Operations Manual
Chapter 3 Preparations
3. Key Functions
Seg. ▲ ▼
To select procedure Section: five Section points as S1,S2,S3,S4,S5.
Temperature setting key. Pressing“ ▲ ”or“ ▼ ”to set running , To set temperature though pressing “▲”or“▼”continuously, which is more fast and conveniently.
Temp. ▲ ▼ temperature
Speed ▲ ▼
Speed setting key. Pressing“▲”or“▼”to set mixing speed,To set mixing speed though pressing “▲”or“▼”continuously, which is more fast and conveniently. Each time the key is pressed , the mix speed changes by 10rpm.
Time ▲ ▼
Timing setting key. Pressing“▲”or“▼”to set timing hours,To set timing hours though pressing “▲”or“▼”continuously, which is more fast and conveniently.
Short Mix
Start/Stop
The device mixes at the frequency visible in the display for as long as the “Short Mix” key is held down. The time is counted in seconds until 999S has expired.
Stop/start key. Pressing this key to stop or start the procedure. Pressing momentary to start, Pressing continuously to stop.
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MS-100 Thermo Shaker Operations Manual
Chapter 4 Operation Guide
Chapter 4 Operation Guide 1. Single temperature, speed and timing setting a)
The LCD will display the picture as the chart when the instrument powers on and the instrument goes into the initial state with the
System-Testing ...
sound of “du…”. b) About 6 seconds,the display window for practical temperature shows 30.0, which is the
S1 30.0 0 00:00 S1 37.0 1000 10:00
practical temperature of the block. Setting temperature
shows
37.0,
which
is
the
establishment temperature. While 10:00 is the
Temp ▲ ▼
former timing time, and S1 is the former temperature section. The temperature unit is “℃”, the speed unit is “RPM” and the time
S1 30.0 0 00:00 S1 40.0 1000 10:00
unit is “hour:minute”.
c) Pressing
Temp’s “▲”or“▼”,the value of
display windows for setting temperature will increase or reduce from decimal digit, unit digit, tens digit to hundreds digit.
Pressing Speed or Time’s “▲”or“▼” ,to set mix speed or timing time according to the
Speed ▲ ▼
S1 30.0 0 00:00 S1 40.0 1200 10:00
Time ▲ ▼
same transformation rule above.
Besides, pressing “▲”or“▼” for 2 seconds continuously to amend the digit from decimal to unit ,from unit to tens digit ,from tens digit to hundreds digit quickly.
S1 30.0 0 00:00 S1 40.0 1200 2:00 Start/Stop Start single temperature procedure
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MS-100 Thermo Shaker Operations Manual
Chapter 4 Operation Guide
If you want to set temperature to 40.0℃, mix speed to 1200 rpm ,timing time to 2 hour, pressing temp’s “▲” continuously to let the temperature reach 40.0, it will be confirmed and stored at once. Press Speed’s “ ▲ ”
continuously to let the
mix speed reach 1200rpm ,Meanwhile pressing time’s “▼” continuously to let the timing time reach 02:00, it will be confirmed and stored at once too. After finishing setting program S1, pressing “stop/start” key to run S1 program.
Heating begins after Start/Stop has been pressed or one of the Temp. curor keys has been pressed.
d) Pressing seg.’s“▲”or“▼” to select
one
section of S1,S2,S3,S4,S5. Then set the values of
temperature, speed and time
Seg. ▲ ▼
according to step (C). You can set these five points as usual segment point, whenever you want to use,you can transfer one of them.
S2 30.0 0 00:00 S2 50.0 1400 3:00
2. How to shut off the temperature, speed and timing function a) b)
Press Seg’s“▲”or “▼” key to Select one section of the S1,S2,S3,S4,S5. Press Temp’s “ ▼ ” key , the lowest value displayes “OFF” , and the device remeans at room temperature. Also press Speed or Time’s “ ▼ ” key, the lowest value displayes “OFF”, Speed displayes “OFF”, it means no mixing function. Time displayes “OFF” , it means no timing function, timing time is ∞, if start/stop key is pressed, it will display “CON” with flickering .
3. Short Mix The device mixes at the frequency visible in the display for as long as the “Short Mix” key is held down. The time is counted in seconds until 999 seconds has expired. The max. Short Mix speed can be set according to your requirement at the current section.
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Short Mix 600rpm
is
running 023S
MS-100 Thermo Shaker Operations Manual
Chapter 4 Operation Guide
4. Temperature calibration The temperature of the Instrument has been adjusted before it is sold out. But if there is deviation between the actual temperature and the displayed temperature due to some reasons, you can do as follows to correct the error. Notes: The Instrument uses two temperatures adjustment to ensure its veracity. This means it is linearly adjusted on 40℃ and 100℃ two points. The temperature veracity will be within ±0.5℃ after the double temperature adjustment. Both the circumstances and the block temperature should be lower than 30℃. Adjustment methods as follows: a) After the startup of the Instrument, it enters waiting interface. Make sure the temperature in display is below 30℃. If the temperature is higher than 30℃, you should wait until the temperature is below 30℃. b) Inject olefin oil into one of the cone-shaped wells, and then put a thermometer into this well (Make sure the precision of the thermometer should be within 0.1℃ and the temperature ball should be absolutely immerged into the cone-shaped well).. Seeing from Fig a.:
Fig a
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MS-100 Thermo Shaker Operations Manual
Chapter 4 Operation Guide
Notes: Please read the actual value after 20minutes’ constant temperature to ensure the adjustment precision.
P:20.5 AdjTemp=
ADJ 40.0
” flicker ceaselessly together
P:40.1 AdjTemp=
ADJ 40.0
d) After 20 mintues,the actual temperature of Thermometer is 38.0℃.Pressing temp’s“▲”or “ ▼ ”key ,amend the value of “Adjtemp” to reach 38.0, then pressing “stop/start”key to confirm.
P:40.1 AdjTemp=
ADJ 38.0
c) Pressing Seg’s“ ▲ ”and“ ▼ ”simultaneously, practical temperature shows 20.5, and rise to 40.0 at once,at the same time the sign“ flicker
ceaselessly.
When
the
”
practical
temperature reach 40.0, the sign “ADJ” and “
Press “stop/start” Then rise to 100.0℃ automatically and “ flicker ceaselessly.
”
e) When the practical temperature reach 100.0, the sign “ADJ” and “
” flicker ceaselessly
P:45.1 ADJ AdjTemp= 100.0
P:100.1 AdjTemp=
ADJ 100.0
P:100.1 AdjTemp=
Adj 98.0
together.
f) After 20 mintues,the actual temperature of Thermometer is 98.0℃.Pressing temp’s“▲”or “ ▼ ”key ,amend the value of “Adjtemp” to reach 98.0. then pressing “stop/start”key to confirm.
Press “stop/start”
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MS-100 Thermo Shaker Operations Manual
Chapter 4 Operation Guide
After Temperature calibration, the temperature displayed is the same with the practical temperature of block.
S1 S1
30.0 0 00:00 50.0 1400 3:00
Note! During Temperature calibration, press Seg.’s “ ▲ ”and“ ▼ ” simultaneously to cancel the calibration. The system keeps the former calibration. So don’t press Seg.’s “ ▲ ”and“ ▼ ” simultaneously unless need calibrate the temperature!
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MS-100 Thermo Shaker Operations Manual
Chapter 4 Operation Guide
5.Exchange of block a) Opens the transparent lid and pull out the four screws which fix the block to the heating board with the screwdriver. Spanner
b) Takes out the screws, closes the lid, puts out the block from the main engine
c) Takes another model block, steadily lays aside on the main engine. The block installment holes aim consistently with the main engine installment holes.
d)
Puts the screws into the installment hole, fixes the metal block on the instrument with the spanner.
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Screw
MS-100 Thermo Shaker Operations Manual Chapter 5 Failure analysis and troubleshooting
Chapter 5 Failure analysis and troubleshooting Failure analysis and processing procedures No.
Phenomenon
Possible Causes No power
1
Check the power
No signals on the Broken Fuse display when it is powered on. Broken switch Others
2
3
4
Exchange fuse(250V 3.0A 5X20) Exchange the switch Contact to the seller
The actual and displayed Broken sensor temperatures are quite different. “OPEN” in the temperature display with the alarm of “du...” “SHORT” in the temperature display with the alarm of “du...”
Processing Procedure
Contact to the seller
Temperature sensor are broken or the environmental Contact to the seller temperature is below zero Temperature sensor are broken or the environmental Contact to the seller temperature is below zero
5
No heating or cooling
Broken sensor or Broken TE Contact to the seller module
6
Press invalid
Broken film switch
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Contact to the seller
MS-100 Thermo Shaker Operations Manual
Annex
Annex 1 Wiring Diagram of MS-100 Thermo Shaker (This diagram is just for you reference. If there is any change, excuse for no notice.) 0.5mm2 red L PE N
24V Power
0.5mm2 red L PE N
Earth
1.0 mm2 yellow+green
+24V+ - 24V-
0.5mm2 blue
1.5mm 红色
Plate Heat Sensor
0.2mm2 white 0.2mm2 white
Heat
0.5mm2 brown(HT) 0.5mm2 black(HT)
X8 X8
X3 X3
220V input
+ -
+ -
24V input X2
X5 Flate heat X5
Motor control X9
Main主板 Board
0.5mm2 red 0.5mm2 black 0.2mm2 green
+ -
24V input J1
J2 X9
J3
0.2mm2 brown Motor control
Motor driver
X1
J2 Lid Sensor X1
驱动板
X4
Driver Board Key interface
Lcd interface
X6 X7
Lcd Display
X7 X6
1x5 wire
Lid heat X4
16pin wire
Earth
J1
X2
Flate sensor
Keypad
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J3
blue green white black blue green white red
Motor
Note