CV5030 Operation Manual V2.1 Aug 2008

NOTE The information, numerical data, notes and value judgments contained in this documentation represent the current state of scientific knowledge and state-of-the-art technology as we understand it following thorough investigation in this field. We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to provide our customers with additional copies, updates etc. of this manual. For erroneous statements, drawings, technical illustrations etc. contained in this manual we exclude liability as far as permissible according to the national legal system applicable in each individual case. In particular, no liability whatsoever is accepted for any financial loss or consequential damage caused by or related to compliance with statements or other information in this manual. Statements, drawings, illustrations and other information as regards contents or technical details of the present manual are not to be considered as warranted characteristics of our products.

These are determined only by the contract provisions agreed between ourselves and our customers. Leica reserves the right to change technical specifications as well as manufacturing processes without prior notice. Only in this way is it possible to continuously improve the technology and manufacturing techniques used in our products. This document is protected under copyright laws. All copyrights of this document are retained by Leica Biosystems Nussloch GmbH. Any reproduction of text and illustrations (or of any parts thereof) by means of print, photocopy, microfiche, web cam or other methods-including any electronic systems and media-requires express prior permission in writing by Leica Biosystems Nussloch GmbH. For the instrument serial number and year of manufacture, please refer to the nameplate on the back of the instrument.

© Leica Biosystems Nussloch GmbH

Published by: Leica Biosystems Nussloch GmbH Heidelberger Str. 17 - 19 D-69226 Nussloch Germany Phone: +49 (0) 62 24 143-0 Fax: +49 (0) 62 24 143-200 Internet http://www.histo-solutions.com Leica CV5030 – Robotic Coverslipper

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Table of contents 1.

Important information ... 6

2. 2.1 2.2

Safety ... 7 Safety notes ... 7 Caution notes ... 7

3. 3.1 3.2 3.3

Instrument Components and Specifications ... 10 Overview - instrument components ... 10 Technical data ... 11 Standard delivery-packing list ... 12

4. Initial Commissioning ... 13 4.1 Location requirements ... 13 4.2 Unpacking and installation ... 14 4.3 Preparing and adjusting the instrument ... 14 4.3.1 Removing the transport anchors ... 14 4.3.2 Leveling the instrument ... 15 4.3.3 Vent system ... 16 4.3.4 Installing the dispensing group/dispenser ... 17 4.3.5 Aligning the nozzle relative to the specimen slide ... 19 4.3.6 Nozzle cleaner ... 21 4.3.7 Connecting to the power supply ... 24 4.3.8 Inserting the accessories ... 24 4.3.9 Refilling consumables ... 26 5. Operation ... 27 5.1 Functions of the control panel ... 27 5.2 Key designations and their functions ... 28 5.3 Switching on the instrument ... 30 5.4 The configuration menu ... 31 5.4.1 Configuring parameter SETs ... 32 5.4.2 Setting menu A parameters ... 32 5.4.3 Setting menu B parameters ... 35 5.4.4 Parameter settings for various cover slip mountants ... 37 5.5 Displays – status messages ... 40 5.6 Coverslipping ... 44 5.6.1 Preparation ... 44 5.6.2 Starting the coverslipping process ... 45 5.6.3 Interrupting the coverslipping process ... 46 5.6.4 Process-based interruptions ... 48

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Operating Manual V 2.1 – 08/2008

Table of contents 5.7 CV5030 with transfer station ... 51 5.7.1 Operation as ST5010 – CV5030 workstation ... 51 5.7.2 Operation as ST5020 – CV5030 workstation ... 52 5.7.2 Operation as ST5020 – CV5030 workstation ... 53 5.7.3 Important instructions for operation as workstation ... 54 5.7.4 Interrupting workstation operation ... 55

6. Maintenance and Cleaning ... 56 6.1 Daily start-up of the instrument ... 56 6.2 Daily cleaning and maintenance ... 57 6.2.1 Overview of cleaning measures ... 59 6.2.2 Cleaning and replacing the suction cups ... 60 6.2.3 Cleaning and maintenance instructions for the nozzle cleaner ... 60 6.3 Procedure for changing the cover slip mountant ... 61 6.3.1 Xylene-based coverslipping mountant -> xylene-based coverslipping mountant ... 61 6.3.2 Xylene substitute-based mountant -> xylene-based mountant ... 61 6.3.3 Xylene-based mountant -> xylene substitute-based mountant ... 61 7.

Malfunctions and Troubleshooting ... 62

8. 8.1

Optional Accessories ... 63 Ordering information ... 63

9.

Warranty and service ... 75

10.

EC Declaration of Conformity ... 76

Leica CV5030 – Robotic Coverslipper

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1.

Important information

Symbols in the text and their meanings Caution notes are highlighted in gray and marked by a

warning triangle.

Notes, i.e. important information for the user, are highlighted in gray and marked by the symbol.

Solvents and reagents that are inflammable are identified using this symbol.

(5)

Numbers given in parentheses refer to positions in illustrations.

START

Function keys to be pressed on the touch screen are written in bold capital letters.

Designated use The Leica CV5030 is a robotic coverslipper for coverslipping tissue sections, cells or swabs mounted on specimen slides with cover slips made out of glass using a wide variety of different cover slip mountants. Any other use of the instrument represents an unauthorized operation. Failure to adhere to these instructions may result in an accident, personal injury, damage to the instrument or accessory equipment.

Instrument type All information contained in this Operating Manual applies solely to the device type listed on the cover page. A name plate with serial number is attached to the rear of the instrument. (The serial number is also displayed above the loading door on the front side of the instrument.)

Qualification of personnel • The Leica CV5030 may be operated by trained laboratory personnel only. • All laboratory personnel designated to operate the Leica CV5030 must read this Operating Manual carefully and must be familiar with all technical features of the instrument before attempting to operate the Leica CV5030. Fig. 1

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Operating Manual V 2.1 – 08/2008

2.

Safety

The safety and caution notes in this chapter must be observed at all times. The user should read these notes even if he or she is familiar with the operation and use of Leica equipment.

2.1 Safety notes This Operating Manual contains important instructions and information for the operational safety and maintenance of the instrument. It is an important component of the instrument and must be read carefully prior to setup and use and kept close to the instrument.

The Operating Manual must be expanded by corresponding instructions if this becomes necessary due to existing national regulations on accident prevention and environmental protection in the country where the instrument is operated.

This instrument has been built and tested in accordance with the safety regulations for electrical measuring, control, regulating and laboratory devices. To maintain this condition and ensure safe operation, the user must observe all notes and warnings contained in this Operating Manual.

For current information about applicable standards, please refer to the CE declaration for the instrument on our Internet site: http://www.histo-solutions.com

The protective devices located on the instrument and the accessories must not be removed or modified. The instrument must only be opened and repaired by service technicians authorized by Leica.

2.2 Caution notes The safety devices attached to this instrument by the manufacturer form only the basis for accident prevention. The main responsibility for accident-free operation lies primarily with the businessperson where the instrument is operated, as well as the persons he or she designates for operating, maintaining or repairing the instrument. To ensure perfect operation of the instrument, the following notes and cautions must be observed.

Leica CV5030 – Robotic Coverslipper

7

2.

Safety

Warnings – Transport and installation • The instrument must be transported in an upright position only (use transport anchors!). Two persons are required when lifting or carrying the instrument! • The Leica CV5030 is intended for use in enclosed rooms only. • Connect the instrument to a grounded power socket only using one of the power cables provided. The protective function must not be removed through the use of an extension cord without protective conductor. The instrument automatically detects the voltage/frequency to which it is connected. • The installation location must be well ventilated, and must contain no sources of ignition of any kind. The chemicals to be used in the Leica CV 5030 are easily inflammable and hazardous to health. • Do not operate the instrument in rooms with explosion hazard. • Exposure to extreme temperature changes between storage and installation locations and high air humidity may cause condensation inside the instrument. In this case, a two-hour waiting period must be maintained before switching on the instrument. If the waiting period is not maintained, the instrument may be damaged. • The instrument must be leveled carefully before commissioning (for more information, refer to Chapter 4.3, "Initial Commissioning").

Warnings – Operating the instrument • The Leica CV5030 may be operated by trained laboratory personnel only. • It may only be used according to its intended use and the instructions in this Operating Manual. • In the event of an emergency, switch off the power switch and unplug the instrument from the power supply. • When working with reagents, wear suitable protective clothing (lab coat, gloves, safety glasses). Avoid skin contact with solvents or cover slip mountants. • Make sure that mountant is applied in the correct amount (for additional information, refer to Chapter 5.4, "The configuration menu"). Excess mountant can run from the specimen slide onto the work surface and the transport belt of the bath insert, thus impairing the movement of parts of the instrument (refer also to Chapter 6, "Cleaning and Maintenance"). • During operation, do not block motor-driven components with objects or manual interference. There is a risk of injury from broken glass! • Do not tilt the transfer module manually!

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Operating Manual V 2.1 – 08/2008

2.

Safety

Warnings – Operating the instrument (continued) • Be absolutely certain to operate the instrument either with the active carbon filter provided or with an exhaust hose, as even when the instrument is used according to its designated use, solvents arise that are both hazardous to health and inflammable! • There is a fire hazard when working with open flame (e.g. Bunsen burners) in the immediate vicinity of the instrument (solvent vapors). Therefore, maintain a minimum safety distance of 3 meters!

Warnings – Handling reagents • Take care when handling solvents! When handling the chemicals used in this instrument, always wear gloves and safety glasses. • The reagents used can be toxic and/or flammable. • When using cleaners, comply with the safety instructions of the manufacturer and the safety regulations of your laboratory! • Dispose of used reagents while observing applicable local regulations and the disposal regulations of your company/lab.

Warnings – cleaning and maintenance • Before each cleaning, switch the instrument off and unplug it from the power supply. • Only authorized and qualified service personnel may access the internal components of the instrument for service and repair. • When cleaning the surfaces, do not use scouring powders or solvents containing acetone, chlorine or xylene. • Clean the hood and housing using mild commercial household cleaners. The finished surfaces are not resistant to solvents! • Prevent liquids from entering the interior of the instrument while the instrument is being cleaned or during operation. Material safety data sheets for reagents can be requested from the respective manufacturer of the chemical. Alternatively, they can be downloaded from the following website: http://www.msdsonline.com Leica CV5030 – Robotic Coverslipper

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3.

Instrument Components and Specifications

3.1 Overview - instrument components Unit cover

Output magazine guide

Output magazines Maintenance door

bottle for cover slip mountant

Cover slip magazine

Dispenser Park position

Control panel

On/Off switch

Loading bath Height-adjustable instrument feet

Input door Fig. 2

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Operating Manual V 2.1 – 08/2008

3. Instrument Components and Specifications 3.2 Technical data Electrical connection values Power supply voltage: Power frequency: Max. input power: Fuse: Tripping current: IEC 1010 classifications:

Connections:

100 to 240 V AC, ± 10 % 50 to 60 Hz approx. 100 VA thermoelectric, integrated in the main power switch 5A Protective class 1 Pollution degree 2 Overvoltage category II RS 232C, service interface

Operating conditions Temperature range: Absolute humidity: Storage temperature range: Storage humidity:

10 °C - 35 °C 10 % - 80 % (non-condensing) 5 °C - 55 °C < 80 %

Dimensions and weights Width: Depth: Height: (with lid closed) Weight:

420 mm 600 mm 550 mm approx. 57 kg

Performance Specimen slide throughput: Suitable specimen slides: Suitable cover slips: Cover slip magazine capacity:

1 specimen slide in approx. 9 sec. high-quality specimen slides* 22 - 24 mm x 40 - 60 mm depends on the thickness/composition of the cover slip

Mountant bottle capacity: Max. fill quantity: Application quantity: Mountant types:

250 ml 200 ml individually adjustable all commercial mountants**

* Contact your supplier for additional information! ** Recommendation: Leica CV UltraTM

Leica CV5030 – Robotic Coverslipper

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3.

Instrument Components and Specifications

3.3 Standard delivery-packing list The standard equipment of the Leica CV5030 consists of the following parts: 1 Basic instrument 1 Dispensing group consisting of: 3 Nozzles (21 G, 20 G, 18 G) 1 Dispenser 1 Accessory kit consisting of: 1 Leica brush 1 Allen key, size 3.0 1 single-head wrench, size 13 1 set of power cables: 1 Power cable "UK" ST/BU F-5A 1 Power cable "Germany" 1 Power cable "USA-Canada-Japan" 1 Active carbon filter (xylene) 1 Nozzle cleaner, complete 2 Bottles for cover slip mountant with lids, 250 ml 1 Specimen slide holder 30, plastic, pack of 5 1 Cross spirit level 1 Cover for loading bath 1 Cover slip catch tray 1 Removal magazine 30, pack of 4 1 Loading bath for specimen slide holder 1 Bath insert for Leica specimen slide holder 30 1 Suction cup, pack of 2 2 Cover slip magazines, multi-size TM 40-60 x 22 mm 2 Cover slip magazines, multi-size TM 40-60 x 24 mm 1 Container for priming solution 1 Exhaust hose, 3 m 1 Operating Manual for Leica CV5030

Cat. No. 14 0478 39700 14 0478 39402

14 0478 39734 14 0183 30751 14 0222 04138 14 0330 37628 14 0411 27822 14 0411 13558 14 0411 13559 14 0422 30673 14 0478 40941 14 0464 36537 14 0475 33643 14 0464 37056 14 0478 39584 14 0478 39585 14 0478 39586 14 0478 39592 14 0478 39593 14 0478 39701 14 0478 39748 14 0478 39749 14 0478 39789 14 0478 39820 14 0707 37101

Carefully check the delivery against the packing list and the delivery note. Should you find any discrepancies, please contact your Leica sales office without delay.

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Operating Manual V 2.1 – 08/2008

4.

Initial Commissioning

4.1 Location requirements The location for the Leica CV5030 Robotic Coverslipper must meet the following requirements: • Stable, vibration-free laboratory bench with horizontal and level tabletop and largely vibration-free floor. Avoid vibrations, bright direct light and strong temperature fluctuations. • The robotic coverslipper requires an installation area of approx. 550 x 600 mm. • So that the service door can be opened, at least 25 cm of free room is required to the left of the instrument. • To ensure that the exhaust hose is connected correctly, at least 15 cm of free room must be maintained on the rear side of the instrument. • Room temperature generally between +10 °C and +35 °C. • Relative humidity 80 % maximum, non-condensing. • The AC socket used for the power supply must be close to the instrument and easily accessible. • The instrument should be operated in a fume hood. If this is not possible, insert the active carbon filter and connect the exhaust hose correctly. • The robotic coverslipper is suitable for operation in enclosed rooms only.

• The installation location must be well ventilated, and must contain no sources of ignition of any kind. The chemicals used in the Leica CV5030 are easily inflammable and hazardous to health. • The instrument must not be operated in areas at risk of explosion. • Exposure to extreme temperature changes between storage and installation locations and high air humidity may cause condensation inside the instrument. In this case, a two-hour waiting period must be maintained before switching on the instrument. If the waiting period is not maintained, the unit may be damaged.

Leica CV5030 – Robotic Coverslipper

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4.

Initial Commissioning

4.2 Unpacking and installation

21

Fig. 3

65

20

• Grab hold of the bottom of the instrument on each side (have at least 2 persons do this) and place it on a stable laboratory table. • When doing so, ensure that the instrument is standing on all four feet. • Pull the plastic protective hood off of the instrument by pulling upwards and remove the two adhesive strips (20). • Remove the foam safeguard (21) for the lift. • Open the loading door (65) and remove the foam cover for the loading bath. • Ensure that the provided accessories are complete in accordance with the order.

4.3 Preparing and adjusting the instrument To commission the instrument, carry out the following tasks: • Remove the transport anchors. • Use the following accessories: • Level the instrument. • Mountant bottle • Insert the filter and attach the exhaust hose. • Cover slip catch tray • Install the dispenser group. • Cover slip magazine • Align the nozzle relative to the specimen slide. • Output magazines • Install the nozzle cleaner. • Loading bath • Connect to the power supply. • Refill consumables.

4.3.1 Removing the transport anchors 50

28

14

• Open the service door (28) on the left of the instrument and remove the foam transport anchor (50) located behind the drive for the loading bath.

Fig. 4

Operating Manual V 2.1 – 08/2008

4.

Initial Commissioning

Transport anchor (24) for cover slip transfer module

24

23 Transport anchor (25) for the gripper

22

To remove the safeguards, unscrew and remove screws (22) and (23). Remove the transport anchor (25) while holding the gripper tightly and set it down slowly.

25

25

Fig. 5

We recommend keeping the transport anchors and screws close to the instrument. The parts can be stored (in the clear plastic bag provided) in the service door.

4.3.2 Leveling the instrument

26 Detail 1

• Move the instrument to its final location on the work surface. When doing so, ensure that the instrument is standing on all four feet.

• Open the hood and place the spirit level (26) provided onto the work surface as shown in Fig. 6, detail 1. 27

• By screwing or unscrewing the instrument feet (27) (detail 2 in Fig. 6), make sure the instrument is level in both directions. Check this using the spirit level.

Detail 2

Fig. 6

Leica CV5030 – Robotic Coverslipper

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4.

Initial Commissioning

4.3.3 Vent system Enlarged detail: Installing the exhaust hose (70). The filter cover (30) and filter (32) have been removed to show 33 the connecting piece (33).

70

Either operate the instrument in a fume hood or insert the active carbon filter and connect the exhaust hose correctly. IMPORTANT! For dry coverslipping, the active carbon filter should be replaced twice a year. For operation with xylene in the bath (without fume hood, with exhaust hose), the active carbon filter must be replaced at least four times a year!

30

Inserting the active carbon filter • Unscrew the knurled screw (34) on the service door (28) on the left of the instrument. Now open the service door towards the left (Fig. 7). • Then, unscrew the screw (29) of the filter cover (30) using a size 3 Allen key and pivot the cover upwards. • You can write down the date of use on the filter label (31). • Insert the filter, close the cover (30) and secure it in place using the screw (29). • Finally, close the service door and retighten the knurled screw.

32 31

29 34

28 31

Attaching the exhaust hose (detail in Fig. 7) • For installation, push the exhaust hose (70) onto the pipe (33) as far as it will go; the pipe is on the rear side of the service door (28).

Filter label

Fig. 7

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Operating Manual V 2.1 – 08/2008

4. 4.3.4 Installing the dispensing group/dispenser Dispensing group 37

36 40 35

Initial Commissioning

• Remove the dispensing group (Fig. 8) from the packaging and keep the three nozzles (Fig. 12) in a safe place. • Insert the dispenser (35) into the holder (39) for the prime position (Fig. 10, page 18). • Connect the pressure hose (36) from the cover of the mountant bottle (37) to the compressed air output (41). To do so, press the top white ring (42) downward and insert the hose (36) as far as it will go. Then pull the ring (42) back upwards. The hose is then connected securely.

38

Fig. 8

Enlarged detail:

Compressed air output

42

and

37 41

Socket for dispenser control

34

• Plug the cable (37) from the dispenser valve into the socket (43) and screw it in place using the knurled screw (38) (see detail). • Screw the cover (40) onto the mountant bottle (44, Fig. 10) and plug the mountant bottle into the holder (45) (Fig. 10).

35 45 39 Fig. 9

Leica CV5030 – Robotic Coverslipper

43

When screwing on the lid, make sure that the sealing ring in the lid of the mountant bottle fits correctly!

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4.

Initial Commissioning

Installing the dispensing group/dispenser (continued)

45 49

48

46

44

Fig. 10

• Finally, insert the cable and the air hose into the holder (45) provided (Fig. 10)

Scope of delivery of coverslipping nozzles

Nozzle 20 G

Inserting the nozzle (Fig.11) • Select the nozzle to be used for coverslipping from the scope of delivery (Fig. 12). • Insert the nozzle (46) into the nozzle holder (48) from below and rotate one corner (47 in Fig. 12) of the nozzle counterclockwise via the retaining plate (49) (Fig. 11). Make sure that the nozzle fits tightly. Leaky nozzles can cause bubbles when coverslipping.

47

Nozzle 18 G

Fig. 11

Nozzle 21 G Fig. 12

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Operating Manual V 2.1 – 08/2008

4.

Initial Commissioning

4.3.5 Aligning the nozzle relative to the specimen slide Aligning the nozzle is necessary to attain optimum distance to the specimen slide when applying the cover slip mountant. This prevents air bubbles from occurring below the cover slip.

Enlarged detail:

55

52

28

51

53

Fig. 13

55

Follow this procedure to align the nozzle: • De-energize the instrument. To do so, set the main switch to OFF and disconnect the power plug. • Open the service door (28) on the left of the instrument as outlined in Chap. 4.3.3 (see Fig. 7). • Move the dispenser into working position. • If necessary, remove the cover slip catch tray. • By rotating the pulley (51) of the specimen slide outfeed (52), position the outfeed such that one of the highest points (55) (see detail) is exactly below the nozzle (Fig. 14). • If necessary, carefully move the transfer module (53) horizontally by hand accordingly. Caution! Do not tilt the coverslip transfer module manually!

• Now check the vertical clearance from the specimen slide outfeed to the nozzle (Fig. 14). It should be approx. 0.5 - 0.8 mm.

46

Fig. 14

Leica CV5030 – Robotic Coverslipper

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4.

Initial Commissioning

Aligning the nozzle to the specimen slide (continued) To set the distance accurately, for example when nozzles have been replaced, follow this procedure: • Remove the dispenser (35) from the holder (57, Fig. 16) and place it in the park position (= prime position) (39) as shown in Fig. 15. • The Allen screw (54, Fig. 16) determines the distance between the nozzle and the specimen slide. It can be adjusted using a size 3 Allen key. Rotating clockwise decreases the distance; rotating counterclockwise increases it.

35

39

Fig. 15

• The change in height per complete turn (360°) is approx. 0.75 mm in the direction of the turn. • Now turn the screw (54) downwards (clockwise) until the nozzle is exactly at the highest point (55, Fig. 14) (distance = 0 mm). Several attempts may be required for this. • Once this point is reached, return the dispenser to rest position and turn the screw (54) one complete turn counterclockwise using the size 3 Allen key (56).

56

• The distance to the nozzle is thus set to the correct value.

57

• Finally, reconnect the instrument to the power supply.

54 Fig. 16

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Operating Manual V 2.1 – 08/2008

4. 4.3.6 Nozzle cleaner The nozzle cleaner is used to clean excess cover slip mountant off of the nozzle after each specimen slide processed. 3

1

7

5

9 8

4

10

Initial Commissioning

Parts Remove the individual components from the packaging and check them for completeness. The following must be present: • • • • • •

Container (1) with lid (10) Brush (2) (2x) Holder (3) with hollow screw (7) and washer (8) Mounting bracket (4) 2 hollow screws (9) Plastic pipette (5) Size 3 Allen key (6)

6

2

Fig. 17

Assembly The nozzle cleaner consists of a container (for the cleaning fluid), into which a brush is inserted. A felt strip (19) is used to keep the brush moist (using xylene). 2

16

10

19 15

①

➁

③ Fig. 18

• Insert the brush (2) into the container so that the lateral guides (15) (2 each on the left and right) fit into the notches (16) provided.

Leica CV5030 – Robotic Coverslipper

• Now, attach the lid (10) and push it down until it clicks into place.

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