DM1000 and DM1000 LED Operating Manual June 2010

Published June 2010 by/ Herausgegeben Juni 2010 von/ Publiée en june 2010 par : Leica Microsystems CMS GmbH Ernst-Leitz-Strasse 17-37 D-35578 Wetzlar (Germany) Responsible for contents/ Verantwortlich für den Inhalt/ Responsable du contenu rédactionnel : Peter Schmitt, Manuela Jacobsen (Clinical Imaging, Product Management) Holger Grasse (Safety Officer according to MPG §30/ Sicherheitsbeauftragter nach MPG §30/ Responsable de la sécurité conformément à la loi relative aux dispositifs médicaux, §30) In case of questions, please contact the hotline/ Bei Fragen wenden Sie sich bitte an die Hotline/ Pour toute question, contacter notre service d’assistance téléphonique :

Phone/Tel./Tél.

+49 (0) 64 41-29 4253

Fax

+49 (0) 64 41-29 2255

E-Mail/e-mail/courriel

[email protected]

Leica DM1000 Leica DM1000 LED Operating Manual

Copyrights

Copyrights All rights to this documentation are held by Leica Microsystems CMS GmbH. Reproduction of text or illustrations (in whole or in part) by print, photocopy, microfilm or other method (including electronic systems) is not allowed without express written permission from Leica Microsystems CMS GmbH. The instructions contained in the following documentation reflect state-of-the-art technology. We have compiled the texts and illustrations as accurately as possible. Nevertheless, no liability of any kind may be assumed for the accuracy of this manual’s contents. Still, we are always grateful for comments and suggestions regarding potential mistakes within this documentation. The information in this manual is subject to modification at any time and without notification.

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Contents

Contents 1.

Important Notes about this Manual ...

6

2.

Intended Purpose of the Microscope ...

7

3. 3.1 3.2 3.3

Safety Notes ... General Safety Notes ... Electrical Safety ... Disposal ...

8 8 8 9

4.

Overview of the Instrument ... 10

5.

Unpacking the Microscope ... 14

6. 6.1 6.2 6.3 6.4 6.5 6.6

Assembling the Microscope ... Stage ... Condenser ... Tube and Eyepieces ... Objectives ... Light Source - Transmitted Light Axis ... Components for Fluorescence Applications ... 6.6.1 Fluorescence Illuminator ... 6.6.2 106z Lamp Housing ... 6.7 Analyzer and Polarizer* ... 6.8 Lambda Plate Compensator ... 6.9 Optional Accessories ... 6.10 Insertion of the batteries ... 6.11 Connection to the Power Supply ...

16 16 18 19 19 20

7. 7.1 7.2 7.3 7.4

28 28 28 29 31

Startup ... Switching on the Microscope ... Köhler Illumination ... Checking Phase Contrast Rings ... Adjusting the Light Sources ...

21 21 21 24 24 25 27 27

8. Operation ... 35 8.1 Switching on ... 35 8.2 Stages and Object Displacement ... 35

8.3 8.4 8.5 8.6 8.7 8.8 8.9

Focusing ... 36 Tubes ... 37 Eyepieces ... 38 Objectives ... 39 Light Sources ... 40 Aperture Diaphragm ... 40 Field Diaphragm ... 41

9. Contrast Methods ... 9.1 Transmitted Light ... 9.1.1 Brightfield ... 9.1.2 Phase Contrast ... 9.1.3 Darkfield ... 9.1.4 Oblique Illumination ... 9.1.5 Polarization ... 9.2 Fluorescence ...

42 42 43 44 44 45 45 46

10. Measurements with the Microscope ... 10.1 Linear Measurements ... 10.2 Thickness Measurements ... 10.3 Differentiation of Gout / Pseudo Gout ...

47 47 48 49

11. Trouble Shooting ... 51 12. Care of the Microscope ... 12.1 Dust Cover ... 12.2 Cleaning ... 12.3 Handling Acids and Bases ... 12.4 Changing Fuses ...

54 54 54 55 55

13. Essential Wear and Spare Parts ... 56 14. Retrofitting Components ... 57 14.1 Equipping the Condenser Disk ... 57 15. Index ... 59 16. EC Declaration of Conformity ... 60

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1. Important Notes about this Manual

1. Important Notes about this Manual Caution! This operating manual is an essential component of the microscope, and must be read carefully before the microscope is assembled, put into operation or used.

This operating manual contains important instructions and information for the operational safety and maintenance of the microscope and accessories. Therefore, it must be kept and taken care of.

Text symbols, pictograms and their meanings: (1.2)

Numbers in parentheses, such as "(1.2)", correspond to illustrations (in the example, figure 1, item 2).

→ p. 20

Numbers with pointer arrows (for example → p. 20), point to a certain page of this manual. Caution! Special safety instructions within this manual are indicated with the triangle symbol shown here, and have a gray background.

!

Caution! The microscope and accessories can be damaged when operated incorrectly.

Notes on the disposal of the device, accessories and consumable materials.

Explanatory note * 6

Item not contained in all configurations

2. Intended Purpose of the Microscope

2. Intended Purpose of the Microscope The Leica DM1000/DM1000 LED microscope, to which this user manual belongs, is designed for biological routine and research applications. This includes the examination of samples taken from the human body with a view to provide information on physiological or pathological states or congenital abnormalities, or to determine the safety and compatibility with potential recipients, or to monitor therapeutic measures. The above-named microscope complies with the Council Directive 98/79/EEC concerning in vitro diagnostics. (It also conforms to the Council Directives 2006/95/EC concerning electrical apparatus and 2004/108/EC concerning electromagnetic compatibility for use in an industrial environment.)

Caution! The manufacturer assumes no liability for damage caused by, or any risks arising from using the microscope for other purposes than those for which they are intended or not using them within the specifications of Leica Microsystems CMS GmbH. In such cases the conformity declaration shall cease to be valid.

Caution! These (IVD) devices are not intended for use in the patient environment defined by DIN VDE 0100-710. Neither are they intended for combining with medical devices according to EN 60601-1. If a microscope is electrically connected to a medical device according to EN 60601-1, the requirements defined in EN 60601-1-1 shall apply. Not suitable for examining potentially infectious specimens.

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3. Safety Notes

3. Safety Notes 3.1 General Safety Notes

3.2 Electrical Safety

This safety class 1 (DM1000) or class 2 (DM1000 LED) device is constructed and tested in accordance with EN 61010-2-101:2002 (DM1000/DM1000 LED), EN 61010-1:2001 (DM1000/DM1000 LED), IEC 61010-1:2001 (DM1000/DM1000 LED), IEC 60825-1:2007 (DM1000 LED) EN 60825-1 + A1 + A2:2003 (DM1000 LED), LED Class I (DM1000 LED) safety regulations for electrical measuring, control, and laboratory devices.

General Specifications

Caution! In order to maintain this condition and to ensure safe operation, the user must follow the instructions and warnings contained in this operating manual.

Caution! The devices and accessories described in this operating manual have been tested for safety and potential hazards. The responsible Leica affiliate or the main plant in Wetzlar, Germany must be consulted whenever the device is altered, modified or used in conjunction with non-Leica components that are outside of the scope of this manual. Unauthorized alterations to the device or noncompliant use shall void all rights to any warranty claims and product liability! 8

Microscope For indoor use only. Supply voltage:

90-250 V AC, 50-60 Hz (DM1000) 12 V DC, 1.5 A (DM1000 LED) Power input: 40 W (DM1000) 18 W (DM1000 LED) Fuses: F 3.15 A 250 V (DM1000) Integrated in external power supply unit, not replaceable (DM1000 LED) Ambient temperature: 15-35°C Relative humidity: max. 80% to 30°C Over voltage category: II Pollution degree: 2 Specifications of the external power supply ELPAC POWER SYSTEMS power supply, Model: FW1812 Input: 100-240 V AC 0,5 A 47-63 Hz Output: 12 V DC 1,5 A max. 18 W max. Caution! Use the original power supply only. Other power supplies must not be used.

3. Safety Notes

If the original power supply fails or is damaged, it must be replaced. Repair is not permitted. Original power supplies are available from your Leica branch office or Leica dealer.

Caution! Protect the microscope from excessive temperature fluctuations. Such fluctuations can lead to the accumulation of condensation, which can damage the electrical and optical components. Ambient temperature: 15-35°C.

Caution! The power plug may only be plugged into an outlet equipped with a grounding contact. Do not interfere with the grounding function by using an extension cord without a ground wire. Any interruption of the ground wire inside or outside of the device, or release of the ground wire connection, can cause the device to become hazardous. Intentional ground interruption is not permitted!

Caution! DM1000 only: Never use any fuses as replacements other than those of the types and the current ratings listed here. Using patched fuses or bridging the fuse holder is not permitted. The use of incorrect fuses may result in a fire hazard.

Caution! The microscope's electrical accessory components are not protected against water. Water can cause electric shock.

Caution! DM1000 only: Before exchanging the fuses or lamps, be absolutely certain to switch off the main power switch and remove the power cable.

3.3 Disposal To dispose of the product at the end of its service life, please contact Leica Service or Sales. Please observe national laws and regulations, such as those implementing and enforcing the WEEE EU directive.

Note:

Like other electronic devices, the microscope and its accessory components must not be disposed of as regular household waste.

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4. Overview of the Instrument

4. Overview of the Instrument Leica DM1000/DM1000 LED

Specification Contrast Methods

• Transmitted light: • Incident light:

brightfield, darkfield, phase contrast, polarization fluorescence

Transmitted Light Axis

Leica DM1000: Integrated halogen illumination Leica DM1000 LED: Integrated LED illumination manual adjustment of • Light intensity • Aperture diaphragm • Field diaphragm (only with Köhler kit)

Incident Light Axis (optional)

Incident light fluorescence illuminator for eyepieces with field number up to 20 with • Interchangeable slide with mount for 3 filter systems • Adjusting lens for lamp • Light trap for the suppression of extraneous light • BG38 blue filter and shutter, switchable

Tube

optionally with • Fixed or variable viewing angle • Up to 3 switching positions • one or two camera ports • Ergotube with height-adjustable eye level and camera port

Magnification Changer (optional)

• Manual • Magnification steps: 1x; 1.5x; 2x

Objective Turret

• Manual • 5-fold for objectives with M25 thread

X/Y Stage

• With condenser holder • Coaxial pinion, optional telescopable • Controls mountable left or right

10

4. Overview of the Instrument

Specification

Leica DM1000/DM1000 LED

Condenser

optionally with • CL/PH 0.90/1.25 OIL condenser with color coding • CLP/PH 0.85 condenser for polarization • Achr.apl. A 0.9 (P) condenser with swivelable condenser head • UCL 0.90/1.25 OIL universal condenser UCLP 0.85 for polarization with 5-position light ring disk) • UCL/P pol. universal condenser with interchangeable condenser head and condenser disk with 6 positions

Focusing

• Focus wheel for coarse and fine focusing • Height adjustment

11

4. Overview of the Instrument

6 5

4

1 2

Fig. 1 Left side of the Leica DM1000 stand 1 Coarse and fine focusing 2 Condenser height adjustment 3 Brightness control 4 Field diaphragm 5 Aperture diaphragm 6 Condenser

12

3

4. Overview of the Instrument

1

2

3

4

5

6

7 8 9

10

Fig. 2 Right side of the Leica DM1000 stand 1 Eyepiece 2 Eyepiece tube 3 Tube 4 Objective turret with objectives 5 Specimen stage with specimen holder 6 Integrated illumination 7 On/Off switch 8 Condenser height adjustment 9 Coarse and fine focusing 10 Coaxial pinion for x/y stage movement

13

5. Unpacking the Microscope

5. Unpacking the Microscope First, carefully remove all components from the transportation and packaging materials.

Note: If at all possible, avoid touching the lens surfaces of the objectives. If fingerprints do appear on the glass surfaces, remove them with a soft leather or linen cloth. Even small traces of finger perspiration can damage the surfaces in a short time. See the chapter "Care of the Microscope" → p. 54, for additional instructions.

Caution!

Installation Location Work with the microscope should be performed in a dust-free room, which is free of vapors (oil, chemicals etc.) and of extreme humidity. At the workplace, large temperature fluctuations, direct sunlight and vibrations should be avoided. These conditions can distort measurements and micrographic images. Allowable ambient conditions Temperature 15-35°C Relative humidity maximum 80% up to 30°C Microscopes in warm and warm-damp climatic zones require special care in order to prevent the build up of fungus. See the chapter "Care of the Microscope" → p. 54, for additional instructions.

Do not yet connect the microscope and peripherals to the power supply at this point! Caution! Electrical components must be placed at least 10 cm away from the wall and away from flammable substances.

14

5. Unpacking the Microscope Transport For shipping or transporting the microscope and its accessory components, the original packaging should be used. As a precaution to prevent damage from vibrations, the following components should be disassembled and packaged separately: • Unscrew the objectives • Remove the condenser • Remove the coaxial pinion • Remove the lamp housings • Disassemble the burner of 106z lamp housing • Remove all moving or loose parts

15

6. Assembly

6. Assembling the Microscope The microscope components are logically assembled in this order: • Stage • Condenser • Fluorescence illuminator* • Intermediate systems* • Tube • Eyepieces • Objectives • Lamp housings with light sources* • Polarization equipment* Only one commonly used screwdriver is necessary for assembly, which is included in the delivery package. The tool can be stored on a magnetic retainer on the underside of the stage at the right. When using intermediate systems and optical accessories, the sequence may vary. In this case, read Chapter "6.9 Optional Accessories" → p. 25. 6.1 Stage

!

Caution:

Before completing the stage, make sure no objectives are installed! Remove the srew located under the stage in the front.

Coaxial Pinion

Note: The coaxial pinion can be mounted on the leftor right-hand side. • First, place the flat fine focus wheel on the side to which you intend to mount the coaxial pinion; the wheel is held in place magnetically (4.1); ensure that the button snaps into place. Attach the other focus knob on the opposite side • Loosen the lock screw (5.1) at the front lefthand side of the stage • Slide the stage as far back as possible • Attach the coaxial pinion with the srew (6.1) • Return the stage to the starting position and retighten the lock screw; after installation of the stage control, move object guide all the way to the left side of the instrument; keep turning when guide has reached the end of travel until a click noise is heard Fig. 3 Specimen stage with specimen holder 1 Lock screws for specimen holder

1

Specimen Holder • Place the specimen holder on the stage and fasten it with the two screws (3.1)

16

6. Assembly Adjusting the Focus Stop The focus stop is preadjusted by the factory to prevent collision with objectives. The focus stop should be set approx. 0.3 mm higher as the focal plane to enable focussing of the samples of different thickness. If a readjustment is necessary, adjust as following: • Lower the stage by rotating 1/2 turn of the coarse adjustment knob • Loosen the focus stop screw on left hand side of microscope

• Loosen the locking screw (5.1) on the left side underside of stage and move the stage all the way back • Loosen the screw for coaxial pinion (6.1), remove the coaxial pinion, and attach the stage lock with this screw • Loosen the stage lock screw and pull the stage forward to the desired position • Retighten the locking screw (5.1) underside of stage • Press the pinion of the stage lock against the rack and retighten the stage lock screw

• Move stage to desired focal plane (preferably approx. 0.3 mm higher) Fig. 5 Underside of stage 1 Lock screw

• Tighten focus stop screw

Stage Lock* The stage lock is mounted in the same hole as the stage drive (in case of ErgoStages, it can be mounted on the opposite position in addition to stage drive). The mounting of stage lock is similar to mounting of the stage drive:

Fig. 4 Focus wheel 1 Magnetic retainer for fine focus wheel

1

Fig. 6 Coaxial pinion installation 1 Mounting screw for coaxial pinion

1

1

17

6. Assembly 6.2 Condenser • If present, screw the condenser head into the condenser • Using the condenser height adjuster (9.3), turn the condenser holder (fig. 8) completely downward • Unscrew the clamping screw for the condenser (9.2) far enough so that the condenser can be inserted from the front

Note: The condenser must be centered before using the microscope. → Köhler illumination p. 28.

Fig. 8 Condenser holder 1 Guiding notch

• From the front, insert the condenser into the condenser holder as far as it will go; on the underside of the condenser, there is an orientation pin (7.1), which must be located in the guiding notch (8.1) • Pull the condenser's clamping screw (9.2) so that the condenser is locked in place

1

Fig. 9 Condenser holder 1 Condenser centering bolts 2 Clamping screw for condenser 3 Condenser height adjuster Fig. 7 Underside of condenser (example CL/PH) 1 Orientation pin 2 Auxiliary condenser lens LS

1

1

3

3

1

2 1

2

18

6. Assembly 6.3 Tube and Eyepieces

• Loosen the clamping screw (11.1) on the stand

Note: For fluorescence applications, install fluorescence illuminator* first → p. 21.

the

If available, the analyzer* (10.1) must be inserted into the stand. This requires that the guide key engages in the guide pin (10.2). To mount the analyzer, the analyzer mount TL* 20 mm or 60 mm can also be placed between stand and tube.

• Insert the tube in the circular receptacle (dovetail ring) • Retighten the clamping screw (11.1) • The eyepieces are inserted into the eyepiece tubes on the tube

6.4 Objectives

An intermediate tube pole* with a switchable analyzer (on/off) and Bertrand lens is also available as an option.

Always only use Leica objectives of tube length ¥ (infinity)! The standard thread is M25. The objectives should be arranged so that the magnification increases when the objective nosepiece is rotated counter-clockwise.

The tube is mounted to the stand either directly or with the use of intermediate modules*.

!

Attention:

Lower the specimen stage as far as possible before assembling the objectives. Close vacant threads in the nosepiece with dust protection caps! Fig. 10 Analyzer mounting 1 Analyzer 2 Orientation pin and guiding notch 3 Clamping screw

Fig. 11 Fastening the tube 1 Clamping screw

1

19

6. Assembly 6.5 Light Source for the Transmitted Light Axis Caution! Note: The Leica DM1000 LED is equipped with integrated LED illumination. The service life of the LED is about 100.000 hours. If, despite this, it should be necessary to change the LED, this task must be carried out by Technical Service only. The following instructions in this chapter refer to the Leica DM1000 with tungsten halogen lamp.

Caution!

Light sources pose a potential irradiation risk (glare, UV-radiation, IR-radiation). Therefore, lamps have to be operated in closed housings. Replacing the Lamp of the Integrated Illumination The transmitted light illumination with a lowvoltage tungsten halogen lamp (fig. 12) is integrated in the base of the microscope and is accessible from the right-hand side. • Remove the insert (12.2)

Be sure that the microscope and lamp housing are disconnected from the power supply. Unplug the power plug and the power supply during assembly.

Caution! The lamp may still be hot! • Remove the lamp

Caution! Fig. 12 1 2

Transmitted-light illumination in Leica DM1000 microscope base Tungsten halogen lamp Insert

Do not remove the new lamp's dust cover until you have installed the lamp. Avoid fingerprints on the lamp. • Insert the new lamp with the dust cover straight into the socket until it stops; be sure that the lamp is inserted straight • Remove the lamp's dust cover • Replace the insert (12.2)

1 20

2