DM6000 B and M Operating Manual Jan 2004

Published January 2004 by: Leica Microsystems Wetzlar GmbH Ernst-Leitz-Straße D-35578 Wetzlar (Germany) Responsible for contents: Karin Schwab (Marketing CM, Compound Microscopy, Product Management) Holger Grasse (Safety Officer according to MPG §30) In case of questions, please contact the hotline:

Phone

+49(0)6441-292286

Fax

+49(0)6441-292255

E-mail: [email protected]

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Leica DM6000B Leica DM6000M Operating Manual

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Copyrights

Copyrights All rights to this documentation are held by Leica Microsystems Wetzlar GmbH. Reproduction of text or illustrations (in whole or in part) by print, photocopy, microfilm or other method (including electronic systems) is not allowed without express written permission from Leica Microsystems Wetzlar GmbH. The term "Windows" can be used in the following text without further identification. It is a registered trademark of the Microsoft Corporation. Otherwise, no inference with regard to the free usability of product names may be drawn from the use of those names. The instructions contained in the following documentation reflect state-of-the-art technology and knowledge standards. We have compiled the texts and illustrations as accurately as possible. Nevertheless, no liability of any kind may be assumed for the accuracy of this manual’s contents. Still, we are always grateful for comments and suggestions regarding potential mistakes within this documentation . The information in this manual is subject to modification at any time and without notification.

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Contents

Contents 1.

Important Notes about this Manual ...

7

2.

Intended Purpose of Microscopes ...

8

3. Safety Notes ... 9 3.1 General Safety Notes ... 9 3.2 Electrical Safety ... 10

7. 7.1 7.2 7.3 7.4 7.5 7.6

4.

Overview of the Instrument ... 12

5.

Unpacking the Microscope ... 18

6. 6.1 6.2 6.3 6.4 6.5

20 21 22 23 24

Assembling the Microscope ... Stage ... Condenser ... Tube and Eyepieces ... Objectives ... Light Sources for the Transmitted Light Axis ... 6.6 Light Sources for the Incident Light Axis ... 6.7 Equipping the Incident Light Turret Disc ... 6.8 Polarizer and Analyzer ... 6.9 DIC Prisms ... 6.10 Optional Accessories ... 6.11 Connecting the Leica CTR6000 Electronics Box ... 6.12 Connecting the Computer ... 6.13 Connection to the Power Supply ...

24 26 31 32 33 34

7.7 7.8 7.9 8. 8.1 8.2 8.3 8.4 61 8.5 8.6 8.7 8.8 8.9

Startup ... Functional Principle ... Switching on ... The LeicaScreen ... The Function Keys at the Stand ... The Remote Control Element SmartMove ... Köhler Illumination ... 7.6.1 Transmitted Light ... 7.6.2 Incident Light ... Checking Phase Contrast Rings ... Adjusting the motorized Polarizer ... Adjusting the Light Sources ...

38 38 43 44 45

Operation ... Switching on ... Stages and Specimen Displacement ... Focusing ... Tubes ...

57 57 59 60

Eyepieces ... Objectives ... Magnification Changer ... Light Sources ... Aperture Diaphragm and Field Diaphragm ...

63 63 66 66

46 46 46 48 49 51 51

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36 37 37

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Contents

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Contrasting Methods for Leica DM6000 B ... 9.1 Transmitted Light ... 9.1.1 Bright Field ... 9.1.2 Phase Contrast ... 9.1.3 Dark Field ... 9.1.4 Polarization ... 9.1.5 Differential Interference Contrast ... 9.2 Fluorescence ... 9.3 Combi Mode ...

11. Trouble Shooting ... 78 68 68 68 69 69 70

12. Care of the Microscope ... 12.1 Dust Cover ... 12.2 Cleaning ... 12.3 Handling Acids and Bases ... 13.

71 72 73

81 81 81 82

Essential Wear and Spare Parts ... 83

14. Abbreviations and Pictograms ... 84 15. Index ... 86

10. Contrasting Methods for Leica DM6000 M ... 10.1 Incident Light ... 10.1.1 Bright Field ... 10.1.2 Dark Field ... 10.1.3 Polarization ... 10.1.4 Interference Contrast ... 10.1.5 Fluorescence ... 10.2 Transmitted Light ... 10.2.1 Bright Field ... 10.2.2 Polarization ...

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74 74 74 74 75 76 76 77 77 77

16. EU Declaration of Conformity ... 87

1. Important Notes about this Manual

1. Important Notes about this Manual Caution! This operating manual is an essential component of the microscope, and must be read carefully before the microscope is assembled, put into operation or used.

This operating manual contains important instructions and information for the operational safety and maintenance of the microscope and accessories. Therefore, it must be kept and taken care of. For the operation of the LeicaScreen and the Software Leica DMControl, please see separate operating manual.

Text symbols, pictograms and their meanings: (1.2)

Numbers in parentheses, such as "(1.2)", correspond to illustrations (in the example, Figure 1, Item 2).

→ p.20

Numbers with pointer arrows (for example → p.20), point to a certain page of this manual. Caution! Special safety instructions within this manual are indicated with the triangle symbol shown here, and have a gray background.

!

Caution! The microscope and accessories can be damaged when operated incorrectly.

Explanatory note.

*

Item not contained in all configurations.

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2. Intended Purpose of Microscopes

2. Intended Purpose of Microscopes The Leica DM6000 B microscope, to which this user manual belongs, is designed for biological routine and research applications. This includes the examination of samples taken from the human body with a view to providing information on physiological or pathological states or congenital abnormalities, or to determining the safety and compatibility with potential recipients, or to monitoring therapeutic measures. The Leica DM6000 M microscope is designed for examinations in the field of materials research. All the above-named microscopes comply with the Council Directive 98/79/EEC concerning in vitro diagnostics. They also conform to the Council Directives 73/23/EEC concerning electrical apparatus and 89/336/EEC concerning electromagnetic compatibility for use in an industrial environment.

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Caution! The manufacturer assumes no liability for damage caused by, or any risks arising from using the microscopes for other purposes than those for which they are intended or not using them within the specifications of Leica Microsystems Wetzlar GmbH. In such cases the conformity declaration shall cease to be valid.

Caution! These (IVD) devices are not intended for use in the patient environment defined by DIN VDE 0100-710. Neither are they intended for combining with medical devices according to EN 60601-1. If a microscope is electrically connected to a medical device according to EN 60601-1, the requirements defined in EN 60601-1-1 shall apply.

3. Safety Notes

3. Safety Notes 3.1 General Safety Notes This safety class 1 device is constructed and tested in accordance with EN 61010-2-101:2002, EN 61010-1:2001, IEC 1010-1:2001, safety regulations for electrical measuring, control, and laboratory devices.

Caution! In order to maintain this condition and to ensure safe operation, the user must follow the instructions and warnings contained in this operating manual.

Caution! The devices and accessories described in this operating manual have been tested for safety and potential hazards. The responsible Leica affiliate or the main plant in Wetzlar must be consulted whenever the device is altered, modified or used in conjunction with non-Leica components that are outside of the scope of this manual. Unauthorized alterations to the device or noncompliant use shall void all rights to any warranty claims!

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3. Safety Notes 3.2 Electrical Safety Caution!

General specifications Leica CTR6000 electronics box For indoor use only. Supply voltage: Frequency: Power input: Fuses: Ambient temperature: Relative humidity: Overvoltage category: Pollution degree:

90-250 V~ 50-60 Hz max. 290 VA T6,3 A (IEC 60127-2/3) 15-35°C max. 80% to 30°C II 2

Microscope For indoor use only. Supply voltage: Frequency: Power input: Fuses: Ambient temperature: Relative humidity: Overvoltage category: Pollution degree:

The power plug may only be plugged into an outlet equipped with a grounding contact. Do not interfere with the grounding function by using an extension cord without a ground wire. Any interruption of the ground wire inside or outside of the device, or release of the ground wire connection, can cause the device to become hazardous. Intentional ground interruption is not permitted!

Caution! 90-250 V~ 50-60 Hz See CTR6000 See CTR6000 15-35°C max. 80% to 30°C II 2

Through activating to the grounding connection (earth screw on the back of the Leica CTR6000 Electronics Box) ancillary equipment with its own and/or extra power supply may be brought to the same ground wire potential. For connections without a ground connector, Leica Service must be consulted.

Supply unit ebq 100* For indoor use only. Supply voltage: Frequency: Power input: Fuses: Ambient temperature: Relative humidity: Overvoltage category: Pollution degree: (see enclosed manual)

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90-250 V~ 50-60 Hz max. 155 VA 2xT2A (IEC 127) 10-36°C max. 80% to 30°C II 2

Caution! Never use any fuses as replacements other than those of the types and the current ratings listed here. Using patched fuses or bridging the fuse holder is not permitted.

3. Safety Notes

Caution! The microscope’s electrical accessory components are not protected against water. Water can cause electric shock.

Caution! Protect the microscope from excessive temperature fluctuations. Such fluctuations can lead to the accumulation of condensation, which can damage the electrical and optical components. Ambient temperature: 15-35°C.

Caution! Before exchanging the fuses or lamps, be absolutely certain to switch-off the main power switch and remove the power cable.

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4. Overview of the Instrument

4. Overview of the Instrument Specification

Leica DM6000 B

Leica DM6000 M

Contrasting Method

• transmitted light: BF, DF, PH, ICT (DIC), Pol • incident light: Fluo

• transmitted light: BF, Pol • incident light: BF, DF, ICR (DIC), Pol, Fluo

Transmitted Light Axis

Incident Light Axis

Tube

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• automatic Illumination Manager (automatic aperture diaphragm and field diaphragm, automatic intensity control) • automatic Constant Color Intensity Control (CCIC) • motorized shutter • integrated into the stand • integrated into the stand • motorized 8x filter turret • motorized 4x filter turret ( 5x optional) (2 fixed positions, 2 variable • with FIM (Fluorescence positions) Intensity Manager) for de• automatic Illumination creasing light intensity in 5 Manager steps • motorized shutter • mechanical “Booster Lens“ (speed < 0.1 s) for increasing fluorescence intensity • Mechanical Excitation Manager to control the fluorescence emission when using double and triple filter cubes. • motorized shutter (speed < 0.1 s) • motorized or manual • up to 3 beam splitting positions • optionally with two camera outputs (for one digital and one analog camera)

4. Overview of the Instrument

Specification

Leica DM6000 B

Leica DM6000 M

Magnification Changer (optional)

• manual • absolute coded • magnification steps: 1x; 1.25x; 1.6x

• manual • absolute coded • magnification steps: 1x; 1.5x; 2x

Objective Turret

• motorized • absolute coded • 7x for objectives with M25 thread • mot. DIC objective prism turret with 4 positions (optional)

• motorized • absolute coded • 6x for objectives with M32 thread • mot. DIC objective prism turret with 4 positions (optional)

X/Y Stage

• motorized, direct stepper motor drive • replaceable specimen stage • zero position defined by end switch • outer dimensions: 234 mm x 157 mm • travel range: 76 mm x 50 mm • smallest increment: 0.3 µm

Condenser

• motorized condenser head • automatic change between contrasting methods: motorized condenser turret for light rings, DF stop, DIC prisms • optional polarizer integrated and motorized • automatic Köhler Illumination

Z Drive

• motorized • dovetail for exchanging stage • travel range: 25 mm • smallest increment: 0.015µm • max. speed: 5 mm/s • min. speed: 1 mm/s • max. load: 4 kg

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4. Overview of the Instrument

Specification

Leica DM6000 B

Leica DM6000 M

Control Panels

• Function keys at the stand • additional variable function keys • focusing knobs • LeicaScreen: touch sensitive LC display 70mm x 70mm, monochromatic • SmartMove: ergonomic control element for x,y,z control with additional variable function keys

Leica CTR6000 Electronics Box

• separate control unit to control • z-drive • xy-stage • objective turret with: • supply voltage for 100W halogen lamp • supply voltage for SmartMove See → p.10 (electrical safety))

Computer Interface

• RS232C

Software Tools

• Leica Application Suite with DMControl for WindowsTM 2000, XP • with plug-ins for: • microscope and camera configuration • microscope and camera control • image acquisition

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4. Overview of the Instrument

Fig. 1

System overview DM6000 B with remote control element SmartMove and electronic box Leica CTR6000

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4. Overview of the Instrument

1 2 3

14 4 5 6

7 13

Fig. 2 1 2 3 4 5 6 7

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12 11 10 9 8

Left side of the stand Leica DM6000 B

Eyepiece Eyepiece tube Motorized tube MBDT Motorized objective nosepiece with objectives Motorized specimen stage with specimen holder Condenser LeicaScreen

8 Function keys field diaphragm 9 Transmitted light/incident light switch 10 Function keys aperture diaphragm 11 Function keys light intensity 12 Focus wheel 13 Variable function keys (factory pre-assigned) 14 Lamp adjustment window

4. Overview of the Instrument

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23 22 21

16

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Fig. 3

Right side of the stand Leica DM6000 B

15 Lamp housing for incident light 16 Lamp housing for transmitted light 17 Transmitted light filter, optional 18 Transmitted light filter, optional 19 Variable function keys (factory pre-assigned) 20 Focus wheel 21 DIC turret 22 Motorized filter turret 23 Magnification changer

Fig. 4 1 2 3 4

19 18

17

Remote control element SmartMove

Movement (x-direction) Movement (y-direction) Focus adjustment Variable function keys (factory pre-assigned)

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1 2

4 17

5. Unpacking the Microscope

5. Unpacking the Microscope The stand box contains the following components:

The CTR6000 Electronics Box, the remote control element SmartMove and the external ebq 100 supply unit* are delivered in separate packaging.

• Stand with integrated incident light axis and objective nosepiece

First, carefully remove all components from the transportation and packaging materials.

The device is delivered in several boxes.

• Specimen stage with stage bracket • Power cable and PC connecting cable • CD with Leica DMControl software package • Instructions and list of microscope default settings („Identification Sheet“) The system box contains the microscope accessories:

Note: If at all possible, avoid touching the lens surfaces of the objectives. If fingerprints do appear on the glass surfaces, remove them with a soft leather or linen cloth. Even small traces of finger perspiration can damage the surfaces in a short time. See the chapter, "Care of the Microscope" → p. 81, for additional instructions.

• Tube • Eyepieces • Objectives • Condenser • Lamp housings with accessories • Fitting tool • Depending on configuration, additional microscope accessories such as filter cubes, etc.

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Caution! Do not yet connect the microscope and peripherals to the power supply at this point!

5. Unpacking the Microscope Installation location

Transport

Work with the microscope should be performed in a dust-free room, which is free of oil vapors and other chemical vapors, as well as extreme humidity. At the workplace, large temperature fluctuations, direct sunlight and vibrations should be avoided. These conditions can distort measurements and micrographic images.

For shipping or transporting the microscope and its accessory components, the original packaging should be used.

Allowable ambient conditions Temperature 15-35°C Relative humidity maximum 80% up to 30°C

• Unscrew the objectives.

Microscopes in warm and warm-damp climatic zones require special care in order to prevent the build up of fungus. See the chapter, "Care of the Microscope" → p. 81, for additional instructions.

• Remove the stage.

As a precaution to prevent damage from vibrations, the following components should be disassembled and packaged separately:

• Remove the condenser.

• Remove the lamp housings. • Disassemble the burner of 106 z lamp housing. • Remove all moving or loose parts.

Caution! Electrical components must be placed at least 10 cm from the wall and away from flammable substances.

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6. Assembly

6. Assembling the Microscope The microscope components are logically assembled in this order: • Stage • Condenser • Tube • Eyepieces • Objectives • Light sources • Filter cubes/reflectors* Only a few commonly used screwdrivers and keys are necessary for assembly, which are included in the delivery package.

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When using intermediate systems and optical accessories, the sequence may vary. In this case, read Chapter "6.10 Optional accessories" → p. 34.