DMi8 A, C and M Installation Manual Rev 1.0 Jan 2015

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Copyright All rights to this documentation are held by Leica Microsystems CMS GmbH. Reproduction of text or illustrations (in whole or in part) by print, photocopy, microfilm or other method (including electronic systems) is not allowed without express written permission from Leica Microsystems CMS GmbH. The instructions contained in the following documentation reflect state-of-the-art technology. We have compiled the texts and illustrations as accurately as possible. Still, we are always grateful for comments and suggestions regarding potential mistakes within this documentation. The information included in this manual may be changed without prior notice.

Revision 1.0, published on 2015-01-15 by: Leica Microsystems CMS GmbH Ernst-Leitz-Strasse 17-37 D-35578 Wetzlar, Germany http://www.leica-microsystems.com Responsible for contents: Marketing CMS 2

Contents

Contents 1. 1.1

Important Notes about this Manual... 4 Text symbols and their meanings... 4

2. 2.1 2.2

Safety Notes... 6 General safety notes... 6 Technical data... 6

3.

Overview of Components... 8

4.

Unpacking... 12

5. Assembling the Microscope... 14 5.1 Assembling the DIC Module and DIC objective prisms... 15 5.2 Assembling the specimen stage... 16 5.3 Inserting the eyepieces... 18 5.4 Inserting the objectives... 18 5.5 Assembling the incident light illuminator... 19 5.5.1 Assembling the LED lamp housing... 19 5.5.2 Assembling the fluorescence illuminator... 19 5.6 Equipping the incident light reflector turret... 20 5.7 Assembling the polarizer and analyzer... 21 5.8 Assembling the transmitted light illuminator arm (TL)... 22 5.8.1 Tiltable transmitted light illuminator arm... 22 5.8.2 Fixed transmitted light illuminator arm... 23 5.9 Assembling the condensers for the tiltable transmitted light illuminator arm... 24 5.10 Assembling the transmitted light lamp housing... 30 5.11 Wiring... 31 5.11.1 Wiring for microscopes with transmitted light illuminator arm... 32 6. Startup... 33 6.1  Adjusting the diaphragms for the incident light illuminator... 33 6.2 Inspecting the phase contrast ring ... 34 6.3 Setting the polarizer... 36 6.4 Condenser centering... 37 7.

Index... 38

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1. Important Notes about this Manual

1. Important Notes about this Manual Caution! This Installation Manual is an essential component of the product; it must be read carefully before the product is assembled, put into operation or used, and must be kept for later reference. This Installation Manual includes important instructions and information related to the operating safety and maintenance of the instrument. 1.1

Text symbols and their meanings (Fig. 1.2)

Numbers in parentheses, e.g. (Fig. 1.2), refer to illustrations, in the example to Fig. 1, Item 2.

→ p.20

Numbers with an arrow, e.g. → p. 20, refer to a specific page of the manual.

WARNING

WARNING indicates a hazard with a medium degree of risk that, if not avoided, can result in death or serious injury.

CAUTION

CAUTION indicates a hazard with a low degree of risk that, if not avoided, can result in minor or moderate injury. Caution! In this manual, additional safety notes are indicated with the triangle symbol shown here, and have a gray background.

!

4

Caution! The instrument and accessories can be damaged when operated incorrectly.

1. IMPORTANT NOTES ABOUT THIS MANUAL

Warning of hazardous electrical voltage! Danger of electric shock! Danger due to hot surface Warning of optical radiation! Never look directly into the light beam! Wear safety goggles! Warning of electromagnetic field Warning of UV radiation! Wear safety goggles! Warning of hazardous laser radiation – take appropriate safety precautions! Warning of hand injuries Instructions for disposing of the instrument, its accessories and consumables. Connection to ground Explanatory note

* MM/YYYY

Item not contained in all equipment configurations Manufacturing date, e.g. 11 / 2011 for November 2011 China RoHS 50 years EFUP (Environmentally friendly use period)

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2. Safety Notes

2. Safety Notes 2.1

General safety notes

2.2

Technical data

Compact Leica CTR electronics box Caution! Make sure to follow the safety notes in the Leica DMi8 User Manual.

For indoor use only. Supply voltage: Frequency: Power consumption: Fuses:

Caution! Also see the instructions and warnings contained in this Installation Manual.

Caution! The instruments and accessories described in this Installation Manual have been safety-tested and checked for possible hazards. The responsible Leica representative or the main plant in Wetzlar must be consulted whenever the device is altered, modified or used in conjunction with non-Leica components that are outside of the scope of this manual! Unauthorized alterations to the instrument or noncompliant use shall void all rights to any warranty claims!

Ambient temperature: Relative humidity: Protection class: Overvoltage category: Pollution degree:

Advanced/Advanced+ Leica CTR electronics box For indoor use only. Supply voltage: Frequency: Power consumption: Fuses: Ambient temperature: Relative humidity: Protection class: Overvoltage category: Pollution degree:

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100 – 240 VAC 50 / 60 Hz max. 150 VA 3.15 A, slow-blowing, breaking capacity H, 250 VAC Size: 5x20 mm 15° - 35°C 90% up to 30°C, non-condensing I II 2

100 – 240 VAC 50 / 60 Hz max. 290 VA 6.3 A, slow-blowing, breaking capacity H, 250 VAC Size: 5x20 mm 15° - 35°C 90% up to 30°C, non-condensing I II 2

2. Safety Notes

Leica DMi8 M microscope (manual)

Leica EL6000*

For indoor use only. Supply voltage: Frequency: Power consumption: Fuses:

For indoor use only. Supply voltage: Frequency: Power consumption: Fuses:

Ambient temperature: Relative humidity: Protection class: Overvoltage category: Pollution degree:

100 – 240 VAC 50 / 60 Hz max. 55 VA 1.6 A, slow-blowing, breaking capacity H, 250 VAC Size: 5x20 mm 15° - 35°C 90% up to 30°C, non-condensing I II 2

100 – 240 VAC 50 / 60 Hz max. 200 VA 2.5 A, slow-blowing, breaking capacity H, 250 VAC Size: 5x20 mm Ambient temperature: 0° - 40°C Relative humidity: 90% up to 30°C, non-condensing Protection class: I Overvoltage category: II Pollution degree: 2 (* See provided Instructions for Use)

Leica DMi8 C microscope (manually encoded) For indoor use only. Supply voltage: Frequency: Power consumption: Fuses: Ambient temperature: Relative humidity: Protection class: Overvoltage category: Pollution degree:

100 – 240 VAC 50 / 60 Hz max. 55 VA 1.6 A, slow-blowing, breaking capacity H, 250 VAC Size: 5x20 mm 15° - 35°C 90% up to 30°C, non-condensing I II 2

Leica DMi8 A microscope (automated variants) For indoor use only. Supply voltage: Frequency: Power consumption: Fuses: Ambient temperature: Relative humidity: Protection class: Overvoltage category: Pollution degree:

100 – 240 VAC (→ Leica CTR) 50 / 60 Hz (→ Leica CTR) see Leica CTR see Leica CTR 15° - 35°C 90% up to 30°C, non-condensing I (→ Leica CTR) II (→ Leica CTR) 2 (→ Leica CTR)

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3. Overview of Components

3. Overview of Components

3

4

2

1 5

6

8

12

Fig. 1:

Right side of the Leica DMi8 A

1 2 3 4 5 6

Objective turret with objectives Tube Eyepieces and eyepiece tubes Specimen stage LED lamp housing Colour filter slot

8

7 8 9 10 11 12

11 10

7

9

Aperture diaphragm Field diaphragm Polarizer slot Analyzer slot Cover for right side-port Cover for motorized incident light turret disk

3. Overview of Components

1

2

4 Fig. 2:

Left side of the Leica DMi8 A

1 2 3 4

Motorized DIC objective prism disk Cover plate (not used) Service port Left side-port with camera

3

9

3. Overview of Components

1

2

3 10 9

8 4

5

7

Fig. 3:

Left side of the Leica DMi8 C

1 2 3 4 5 6 7 8 9 10

Eyepieces and eyepiece tubes Tube Top-port with camera Cover plate (not used) Incident light turret disk Cover for left side-port Manual DIC objective prism disk LED lamp housing Objective turret with objectives Specimen stage

10

6

3. Overview of Components

Variants with transmitted light illuminator arm Fig. 4: Left side of the Leica DMi8 with tiltable transmitted light illuminator arm 1 Tiltable transmitted light illuminator arm 2 Condenser height adjustment 3 Manual polarizer 4 Manual condenser, alternatively: motorized condenser 5 Condenser head 6 Condenser height stop

1

2

3 4

6 Fig. 5: Right side of the Leica DMi8 with tiltable transmitted light illuminator arm 1 Transmitted light filters 2 Transmitted light lamp housing receptacle 3 Condenser with motorized polarizer

5

1 2

3

Fig. 6: Left side of the Leica DMi8 with fixed transmitted light illuminator arm 1 Stop lever for condenser height adjustment 2 Fixed transmitted light illuminator arm 3 Transmitted light lamp housing 4 Receptacle for transmitted light filter 5 Light ring slide 6 Manual condenser with aperture diaphragm

3 4 2 1

5 6

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4. Unpacking

4. Unpacking The microscope is delivered in several packages depending on instrument configuration and the scope of the system. The Leica CTR electronics box, Leica SmartMove control element, Leica STP4000/8000 control panel, movable stages/ stage accessories and Leica EL6000 compact light source are delivered in separate packages.

Note:

If at all possible, avoid touching optical surfaces such as the lens surfaces on the objective. If fingerprints do appear on the glass surfaces, remove them with a soft leather or linen cloth. Even small traces of finger perspiration can damage the surfaces in a short time. See the chapter on "Care of the Microscope"→ for additional instructions in the Instructions for Use.

Caution! Caution! The microscope may only be set up, installed and put into operation by authorized Leica personnel!

Caution! The microscope may only be operated by personnel who have been instructed by an authorized Leica employee! Please compare the contents of the package you receive carefully with the packing slip, the delivery note or the invoice. We highly recommend keeping a copy of these documents with the manual in order to have delivery date and delivery package information available when (for example) placing repeat orders or making service calls. Please make sure that no small parts are lost in the packaging materials. Some of our packaging materials have symbols to help in environmentally responsible recycling. First, carefully remove all components from the transportation and packaging materials.

Caution! If an instrument or its packaging have visible damage, do not put the instrument into operation.

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Do not connect the microscope and peripherals to the power supply at this point under any circumstances! Installation location Work with the microscope should be performed in a dustfree room, which is free of oil vapors, other chemical vapors and extreme humidity. At the workstation, large temperature fluctuations, direct sunlight and vibrations should be avoided. These conditions can distort measurements and longer microscopic scans. Permitted ambient conditions: Temperature Relative humidity

15–35°C maximum 90% up to 30°C non-condensing

Microscopes in warm and warm-damp climatic zones require special care in order to prevent fungus contamination. For additional instructions, see the chapter on "Care of the Microscope" in the→ User Manual.

Caution! The microscope must be set up so that the power voltage input is not obstructed and the instrument can quickly be removed from the power supply if necessary.

4. Unpacking

Caution! Electrical components must be placed at least 10 cm away from the wall and away from flammable substances.

Weight The weight of the microscope depends on the respective equipment configuration.

CAUTION At a full load, the microscope weighs more than 18 kg. The user must take corresponding precautions for transporting the instrument.

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5. Assembling the Microscope

5. Assembling the Microscope The microscope components +) are logically assembled in this order: • Only for microscopes with transmitted light illuminator arm (chapter 5.8-5.10): • Transmitted light illuminator arm • Condenser with condenser head • Transmitted light lamp housing • DIC module and DIC objective prisms • Specimen stage • Eyepieces • Objectives • Incident light illuminator (LED lamp housing) • Equipment for the incident light turret • Polarizer and analyzer

When using other systems and advanced optical accessories, the sequence may vary. Only a few commonly used screwdrivers and keys are necessary for assembly; these are included in the delivery package.

+) Depending on scope of delivery

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5. Assembling the Microscope – DIC module and DIC objective prisms

5.1 Assembling the DIC Module and DIC objective prisms If the microscope is not equipped with DIC, please jump ahead to Chapter 5.2. In microscopes of the Leica DMi8 M series, the DIC prisms are already installed in the DIC disk (Fig. 7) below the objective turret. Motorized, manually encoded and manual DIC disks are offered. Assembly is identical for all variants.

Fig. 7: DIC objective prism turret (encoded and motorized) 1 IC objective prism in mount 2 Identifying letter (ID) 3 Orientation pin

When upgrading the IC prism disk, follow these steps: • Remove the front panel (Fig. 8) under the objective turret after loosening the Allen screws (Fig. 9). • Insert the DIC prism disk (Fig. 7) evenly into the receptacle. Start by gently turning one screw using the 3 mm hexagonal key provided. Then, tighten both Allen screws.

! Caution!

3

2

1

Fig. 8:

Front panel for DIC prism disk

Fig. 9:

Removing the front panel

Insert the prism disk so that the electronics board is facing downwards. Do not touch the electronics (especially the contacts) with your fingers! Upgrading individual IC prisms: • Loosen both Allen screws and remove the prism disk. • Place the prisms against the peg (Fig. 7.3) and the shim between the prism and screw (Fig. 10) and lightly tighten to avoid tension. Insert the prisms so that the identifying letter, or ID, is facing upwards and is legible. • After assembling the prisms, reinsert the prism disk into the receptacle. Alternatively: Insert the objective prism slide (Fig. 11) into the slot below the objective turret. The code letter must match the code letter on the objective. Fig. 11: Objective prism slide

Fig. 10: IC objective prism 1 Objective prism in mount 2 Washer and screw 2 1

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5. Assembling the Microscope – Specimen stages

5.2

Assembling the specimen stage

Fig. 12: Fixed stage

There is a wide variety of available specimen stages. The accompanying figures depict a few examples. The assembly of each of these stages is identical. The stages are permanently affixed to the microscope using 3 screws (Fig. 14). In the case of fixed stages, a mechanical stage can be attached (Fig. 15).

! Caution! Screw lengths may vary. The following applies to the shipment of different screws: Always use the shorter of the 3 screws for the front hole and both screws of equal length for the rear holes.

Fig. 13: Mechanical 3-plate stage

• Align the stage so that both holes point back towards the illumination axis and the single hole is facing in the direction of the tube. • Align the fastening holes in the stage with the holes in the contact surface. If the holes are not visible, as in the case of the 3-plated cross-stage or scanning stage, gently move the upper tabletop until the opening is visible. • Start by gently turning the front screw using the provided 3 mm hexagonal key. It is important that the shorter of the 3 screws is always placed in the front hole, because a screw that is too long can damage the focus hub.

Fig. 14: a, b assembly screws for 3-plate cross-stage

• Then, tighten both rear screws. • Finally, retighten the front screw.

a

16

b

5. Assembling the Microscope – Specimen stages

Fixed stage

Fig. 15: Mechanical stage for fixed micromanipulation stage

Mechanical stages for fixing holders for various culture flasks are also optionally available for fixed stage plates (Fig. 15). • The mechanical stage has 2 screws. Tighten these screws into the threaded holes on the bottom of the fixed stages using the 3 mm hexagon socket wrench and retighten regularly after frequent use of the mechanical stage. The mechanical stage is preadjusted at the factory. If the mechanical stage shifts out of focus when moving from right to left, this can be corrected by Leica Technical Service.

Fig. 16: Inserts for mechanical stage (fixed stage) For additional examples, see special brochure

• Now, remove the ordered stage insert or inserts (Fig. 16) from the packaging and guide one stage insert into the precise click system. The stage, mechanical stage and stage insert are now ready to use. Self-adhesive scales for reading the coordinate adjustment are included with some (not all) inserts. • Finally, affix these scales in the recesses of the mechanical stage. Motorized 3-plate or scanning stage 3-plate stages and scanning stages: • After assembling the stage, first connect (in the case of motorized stages) the provided stage cable to the socket on the stage and then to the electronics box. The corresponding mark on the box is called "XY- Stage". Various inserts are offered with the normal 3-plate or scanning stages (including heated inserts). • Carefully place these inserts into the corner provided with spring clips from above at an angle. The stage inserts click into place once positioned correctly.

! Caution!

Only press on the spring clips from the sides. Never press on the insert plate at the spring clips from above at an angle, because then the insert will not be plane-parallel to the stage and cannot be bent.

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5. Assembling the Microscope – Eyepiece and objectives

5.3

Inserting the eyepieces

Fig. 17: Eyepieces

• Insert the eyepieces into the eyepiece tubes.

Note:

We recommend using the LAS software to teach in eyepieces that are not included in the scope of delivery. This ensures that the total magnification specified on the touchscreen is correct. See LAS software manual:

5.4

Inserting the objectives

• Screw the objectives into the objective turret. The receptacles on the objective turret are numbered (Fig. 18). Depending on your equipment, specific positions have already been assigned to the individual objectives at the factory. For details on the exact positions of the objectives, please refer to the enclosed "Identification sheet".

Fig. 18: Objective turret

! Caution!

Close vacant threads in the turret using dust protection caps! Please note that the front lenses of the objectives face upwards. This subjects them to increased exposure to contamination compared to objectives on upright microscopes. Check the front lens more often for cleanliness for this reason.

Note:

We recommend carrying out a parfocality adjustment via the LAS software. See LAS software manual:

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Fig. 19: Objective turret, equipped

5. Assembling the Microscope – Illumination

5.5

Assembling the incident light illuminator

5.5.2 Assembling the fluorescence illuminator Leica EL6000

Caution! Ensure that the lamp housing has been disconnected from the power supply. Unplug the power plug and the power supply during assembly.

See separate manual.

CAUTION

CAUTION

When using the Leica EL6000 compact light source, it is essential to observe the safety notes in the separate instructions included.

Light sources pose a potential irradiation risk (blinding, UV radiation, IR radiation). Therefore, lamps may only be operated in closed housing and in an installed condition. Never look directly into the beam path (blinding hazard).

CAUTION

5.5.1 Assembling the LED lamp housing • Place the lamp housing into the incident light lamp housing receptacle (Fig. 20) and fasten it with the side clamping screw. Leica DMi8 M with manual incident light method: • Connect the cable of the lamp housing to the port (Fig. 21.1) on the rear side of the microscope. Leica DMi8 A and C with motorized incident light method: • Connect the cable of the lamp housing to the power supply for transmitted light for the Leica CTR electronics box ("Lamp RL" port). Fig. 20: Fastening the lamp housing to the incident light axis 1 Fastening screw

Just switch on the light source once the light guide is connected to the microscope. Uncontrolled light output from the light guide poses a blinding hazard! There is also a burn hazard at the light guide output! • Screw the Leica EL6000 microscope adapter onto the rear side of the microscope in the lamp housing receptacle. • Close the light guide according to the information in the Leica EL6000 manual.

Fig. 21: Manually connecting the lamp housing for the Leica DMi8 1 Incident light lamp housing connection

1

1

19

5. Assembling the Microscope – Incident Light turret equipment

5.6

Equipping the incident light reflector turret

! Caution!

Fig. 22: Access to turret disk 1 Cover

First, completely read through this section before you begin equipping the reflector turret disk. The reflector turret disk can be accessed from both the right side and the left side of the microscope (Fig. 22). The positions on the reflector turret disk are numbered. Depending on how they are equipped, the individual filter and/ or reflector cubes are set in pre-assigned positions at the factory. For details, check the identification sheet included with your order.

1 Fig. 23: Equipping the turret disk

• Remove the cover from the reflector turret disk (Fig. 22.1). The cover is secured in place by a magnetic holder and can be detached from the front. • With the holder facing you squarely, slide the filter cube or reflector cube forwards into the holder as described in the identification sheet provided. • Check to ensure that the cube is placed properly. A loose cube can be damaged when rotating the turret disk or can block the disk. • Once all filter blocks and reflector blocks have been inserted, put the cover back in place.

Note:

Is the microscope equipped with a 2-fold filter turret, the turret must be pushed across the arrester position to exchange the filter cubes. It is not possible to insert the filter cubes when the turret is in the arresting position.

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Fig. 24: Turret disk with used filter cubes