DMi8 Instructions for Use Rev 1.1 July 2015

COPYRIGHT/ CONTENTS

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Leica DMi8 Instructions for Use Leica Microsystems CMS GmbH t Instructions 11934056, Revision 1.1 from 2015-07-15

COPYRIGHT/ CONTENTS

Copyright

Contents

All rights to this documentation are held by Leica Microsystems CMS GmbH. Reproduction of text or illustrations (in whole or in part) by print, photocopy, microfilm or other method (including electronic systems) is not allowed without express written permission from Leica Microsystems CMS GmbH.

1 1.1

Important Notes about this Manual ... 4 Text symbols and their meanings ... 4

2.

Intended Purpose of the Microscope ... 6

The instructions contained in the following documentation reflect state-of-the-art technology. We have compiled the texts and illustrations as accurately as possible. Still, we are always grateful for comments and suggestions regarding potential mistakes within this documentation.

3. 3.1 3.2 3.3 3.3.1 3.3.2 3.3.3 3.3.3.1

The information included in this manual may be changed without prior notice.

3.3.3.2 3.3.3.3

3.3.4. 3.5 3.6 3.7 3.8 3.9 3.10 3.11

Safety Notes ... 8 General safety notes ... 8 Electrical safety ... 8 Laser safety ... 11 Safety switch (interlock) on the microscope ... 11 Screwed-on filter turret cover... 12 Laser coupling by the user ... 12 Safety hazard due to folded back Transmitted Light arm ... 13 Safety hazard at eyepiece... 13 Safety hazard in the condenser area during operation. ... 13 Safety hazard above the condenser... 14 Safety hazard in the specimen area ... 14 Safety hazard due to openings at microscope ... 15 Note regarding the safety hazards in Sections 3.3.3.1 and Section 3.3.3.2 ... 16 Exception for Class 1 lasers ... 17 Storage and transport ... 18 Ambient conditions... 18 Notes on handling light sources ... 19 Notes on handling the Touch Screen ... 20 Notes on handling immersion oil... 20 Notes on handling acids and bases ... 20 Disposal ... 20

4. 4.1 4.2 4.3 4.4 4.4.1 4.4.2 4.4.3 4.4.4 4.4.5

Leica DMi8 configurations instrument overview 21 Microscope configurations (examples) ... 21 Dimensions (specified in mm)... 22 Specifications+) ... 24 Controls ... 28 Control panel fields... 29 Additional control elements ... 31 The Touch Screen ... 34 The Leica SmartMove control element... 36 Leica STP4000/8000 external control panel... 37

3.3.3.4 3.3.3.5 3.3.3.6 3.3.3.7

Revision 1.1, published on 2015-07-15 by: Leica Microsystems CMS GmbH Ernst-Leitz-Straße 17-37 D-35578 Wetzlar (Germany) http://www.leica-microsystems.com Responsible for contents: Marketing CMS 2

CONTENTS

5. Operation ... 38 5.1 Switching on the microscope ... 39 5.2 Illumination ... 41 5.2.1 Transmitted Light... 41 5.2.2 Fluorescence ... 42 5.2.3 Infinity port ... 44 5.2.4 Shutter ... 45 5.3 Objectives ... 46 5.4 Tube ... 49 5.5 Eyepieces ... 50 5.6 Stages and object displacement... 51 5.6.1 Object displacement ... 51 5.6.2 Adjusting the step sizes on motorized stages... 52 5.7 Focusing ... 54 5.7.1 Focusing the image ... 54 5.7.2 Focus thresholds ... 55 5.7.3 Adjusting the step sizes ... 56 5.7.4 AFC (Adaptive Focus Control) ... 57 5.8 Selecting the ports ... 58 5.9 Magnification Changer ... 59 5.10 Aperture diaphragm and field diaphragm ... 60 5.11 Filter ... 62 5.11.1 Transmitted Light filter ... 62 5.11.2 Fluorescence Cubes... 63 5.11.3 Leica RFID Module... 65 5.11.3.1 Product Description ... 65 5.11.3.2 Specifications LID Module 1.0 ...65 5.11.3.3 Specifications RFID Tag D6.7 special ... 65 5.11.3.4 Ports ... 65 5.11.3.5 FCC / IC Compliance ... 65 5.12 The memory function on the Touch Screen ... 66 5.13 Transmitted Light contrast method ... 66 5.13.1 Brightfield (TL) ... 67 5.13.2 Phase Contrast (TL) ... 69 5.13.3 Darkfield (TL) ... 71 5.13.4 Polarization (TL) ... 72 5.13.5 Differential Interference Contrast – DIC (TL) ... 74 5.13.6 Integrated Phase Contrast – IPH (TL) ... 76 5.13.7 Integrated Modulation Contrast – IMC (TL) ... 78 5.14 Fluorescence ... 82 5.15 Combination method ... 85

6.

Troubleshooting ... 86

7. 7.1 7.2 7.3

Care of the Microscope... 89 Dust Cover ... 89 Cleaning ... 89 Handling acids and bases ... 90

8.

Essential Consumable and Spare Parts ... 91

9. 9.1 9.2 9.3 9.4

Appendix ... 92 Assigning the variable function keys ... 92 Abbreviation glossary ... 94 Overview of Touch Screen pictograms ... 95 Additional information... 96

10.

Index ... 97

11.

EC Declaration of Conformity ... 99

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1. IMPORTANT NOTES ABOUT THIS MANUAL

1 Important Notes about this Manual Caution! These Instructions for Use are an essential component of the product; they must be read carefully before the product is assembled, put into operation or used, and must be kept for later reference. These Instructions for Use include important instructions and information related to the operating safety and maintenance of the instrument.

1.1

Text symbols and their meanings (1.2)

Numbers in brackets, e.g. (1.2), refer to illustrations, in the example to Fig. 1, item 2.

o p.20

Numbers with an arrow, e.g. o p. 20, refer to a specific page of the manual.

WARNING

WARNING indicates a hazard with a medium degree of risk that, if not avoided, can result in death or serious injury.

CAUTION

CAUTION indicates a hazard with a low degree of risk that, if not avoided, can result in minor or moderate injury.

Caution! In this manual, additional safety notes are indicated with the triangle symbol shown here, and have a gray background.

!

4

Caution! The instrument and accessories can be damaged when operated incorrectly.

1. IMPORTANT NOTES ABOUT THIS MANUAL

Warning of hazardous electrical voltage! Risk of electrical shock!

Danger due to hot surface Warning of optical radiation! Never look directly into the light beam! Wear safety goggles!

Warning of electromagnetic field

Warning of UV radiation! Wear safety goggles! Warning of hazardous laser radiation – take appropriate safety precautions!

Warning of hand injuries Instructions for disposing of the instrument, its accessories and consumables.

Connection to ground

Explanatory note

*

MM/YYYY

Item not contained in all equipment configurations

Manufacturing date, e.g. 11 / 2011 for November 2011

China RoHS 50 years EFUP (Environmentally friendly use period)

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2. INTENDED PURPOSE OF THE MICROSCOPE

2. Intended Purpose of the Microscope DMi8 Leica microscopes are inverted light microscopes and are intended for use as general lab microscopes for routine examinations of biological specimens. The Leica DMi8 series can be used universally. All contrast methods, such as Brightfield, Darkfield, Phase Contrast, DIC, fluorescence or modulation contrast, are integral components of the microscope and can be adapted or exchanged quickly and easily. Variable illumination and imaging beam paths as well as HCS optics, modular accessories and a wide range of available peripherals supplement the inverted Leica research stand from Leica Microsystems CMS GmbH. Leica DMi8 microscopes: Leica DMi8 manual Leica DMi8 coded Leica DMi8 automated Leica DMi8 automated Leica DMi8 automated

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11889110 11889111 11889112 11889113 11889014

Directives of European Community (EC-Directives) The named microscope meets the requirements of the Council Directives 2006/95/EC and 2014/35/EC concerning electrical apparatus and 2004/108/ EC and 2014/30/EC concerning electromagnetic compatibility.

Reasonably foreseeable misuse The following are prohibited: t
To use the microscope for any purpose not in accordance with the Declaration of Conformity (e.g. the use of medical products in accordance with EU Directive 93/42/EEC) t
Making use of auxiliary equipment to use the area between the microscope stage and objectives as a clamp or holder (working like a vice) t
Operating the microscope in an inclined position t
Cleaning the microscope in a way other than specified in the manual t
Modifying the built-in safety circuits in the instrument t
Allowing unauthorized personnel to open the instrument t
Using cables that Leica Microsystems CMS GmbH has not provided or permitted t
Using the swiveling Touch Screen of the microscope as a transport handle t
Using combinations with non-Leica components that go beyond the scope of the manual

2. INTENDED PURPOSE OF THE MICROSCOPE

Caution! The manufacturer assumes no liability for damage caused by, or any risks arising from, using the microscope for other purposes than those for which they are intended or not using them within the specifications of Leica Microsystems CMS GmbH. In such cases, the Declaration of Conformity shall be invalid.

WARNING Notes on handling laser devices In the standard design without additional laser safety precautions, the microscopes are not suitable for coupling laser radiation (such as to the camera ports), since this radiation poses a hazard for the user (eye injury in particular). For use of the microscope with lasers, Leica Microsystems CMS GmbH offers special microscope variants with additional safety features. Laser couplings require corresponding safety devices that have to be inspected and installed by trained personnel. For further information, please contact your authorized Leica Microsystems CMS GmbH representative.

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3. SAFETY NOTES

3. Safety Notes 3.1

General safety notes

These instruments of protection class 1 are built and inspected in accordance with EN 61010-1 /IEC 61010-1 Safety requirements for electrical equipment for measurement, control and laboratory. They also meet the requirements in EN / IEC 61326-1 Electrical equipment for measurement, control and laboratory use – EMC requirements. The products were also tested in accordance with EN 62471 / IEC 62471 Photobiological safety of lamps and lamp systems.

Caution! The instruments and accessories described in this Instructions for Use have been tested for safety and potential hazards. The responsible Leica Microsystems CMS GmbH affiliate or the main plant in Wetzlar must be consulted whenever the device is altered, modified or used in conjunction with non-Leica components that are outside of the scope of this manual. Unauthorized alterations to the instrument or noncompliant use shall void all rights to any warranty claims!

3.2

Electrical safety

General specifications Caution! In order to maintain this condition and to ensure safe operation, the user must follow the instructions and warnings contained in this operating manual.

Compact Leica CTR electronics box For indoor use only. Supply voltage: Frequency: Power consumption: Fuses:

Ambient temperature: Relative humidity: Protection class: Overvoltage category: Pollution degree:

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100 – 240 VAC 50 / 60 Hz max. 150 VA 3.15 A, slow-blowing, Breaking capacity H, 250 VAC Size: 5x20 mm 15° - 35°C 90% up to 30°C, non-condensing I II 2

3. SAFETY NOTES

Advanced/Advanced+ Leica CTR electronics box

Leica DMi8 automated

For indoor use only. Supply voltage: Frequency: Power consumption: Fuses:

For indoor use only. Supply voltage: Frequency: Power consumption: Fuses: Ambient temperature: Relative humidity:

Ambient temperature: Relative humidity: Protection class: Overvoltage category: Pollution degree:

100 – 240 VAC 50 / 60 Hz max. 290 VA 6.3 A, slow-blowing, Breaking capacity H, 250 VAC Size: 5x20 mm 15° - 35°C 90% up to 30°C, non-condensing I II 2

Protection class: Overvoltage category: Pollution degree:

100 – 240 VAC (oLeica CTR) 50 / 60 Hz (oLeica CTR) see Leica CTR see Leica CTR 15° - 35°C 90% up to 30°C, non-condensing I (oLeica CTR) II (oLeica CTR) 2 (oLeica CTR)

Leica EL6000* Leica DMi8 manual For indoor use only. Supply voltage: Frequency: Power consumption: Fuses:

Ambient temperature: Relative humidity: Protection class: Overvoltage category: Pollution degree:

100 – 240 VAC 50 / 60 Hz max. 55 VA 1.6 A, slow-blowing, Breaking capacity H, 250 VAC Size: 5x20 mm 15° - 35°C 90% up to 30°C, non-condensing I II 2

For indoor use only. Supply voltage: Frequency: Power consumption: Fuses:

100 – 240 VAC 50 / 60 Hz max. 200 VA 2.5 A, slow-blowing, Breaking capacity H, 250 VAC Size: 5x20 mm Ambient temperature: 0° - 40°C Relative humidity: 90% up to 30°C, non-condensing Protection class: I Overvoltage category: II Pollution degree: 2 (* See provided Instructions for Use)

Leica DMi8 coded For indoor use only. Supply voltage: Frequency: Power consumption: Fuses:

Ambient temperature: Relative humidity: Protection class: Overvoltage category: Pollution degree:

100 – 240 VAC 50 / 60 Hz max. 55 VA 1.6 A, slow-blowing, Breaking capacity H, 250 VAC Size: 5x20 mm 15° - 35°C 90% up to 30°C, non-condensing I II 2

WARNING The power plug may only be plugged into an outlet equipped with a grounding contact. Do not interfere with the grounding function by using an extension cord without a ground wire. Any interruption of the ground wire inside or outside of the instrument, or release of the protective conductor terminal, can cause the instrument to become hazardous. Intentional ground interruption is not permitted!

9

3. SAFETY NOTES

Caution! By connecting it to the grounding connection (earth screw at the back of the electronics box) ancillary equipment with its own and/or extra power supply may be brought to the same ground wire potential. For connections without a ground protection conductor, Leica Microsystems CMS GmbH Service must be consulted.

Caution! Never use any fuses as replacements other than those of the types and the current ratings listed here. Using other fuses or bridging the fuse holder is not permitted. The use of incorrect fuses may result in a fire hazard.

Caution! The microscope's electrical accessory components are not protected against water. Water can cause electric shock.

Caution! Protect the microscope from excessive temperature fluctuations. Such fluctuations can lead to the accumulation of condensation, which can damage the electrical and optical components. Operating temperature: 15-35°C

WARNING Before exchanging the fuses or lamps, be absolutely certain to switch off the main power switch and remove the power cable.

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! Caution! Only insert or unplug data and control circuits when the instrument is switched off; otherwise, the instrument may be damaged.

WARNING By definition, the main circuit breaker of this instrument is the connection between the power cable and device port. The user must ensure unobstructed access to the main circuit breaker at all times.

WARNING Only use power cables, or alternative cables with VDE/ HAR codes that minimally fulfill the following conditions: 3x0.75 mm2 and 10A/250V.

3. SAFETY NOTES

3.3

Laser safety

3.3.1

Safety switch (interlock) on the microscope

Two safety switches are integrated into the microscope using a laser safety kit option. These must be integrated into the laser safety concept so that the light path of the laser beam is interrupted when the interlock switch is opened.

Two equivalent interlock connectors, which lead to the microscope's interlock switch, are attached to the back of the microscope. The interlock connector of the microscope must be connected to your laser beam interrupter.

Interlock connectors on the rear side of the stand

Technical data for the interlock connector: Plug: Manufacturer: Binder Type: 09-0097-20-05 (5-pin flange plug, 711 series subminiature round plug connector)

1

2

Interlock switch (1) and (2)

Interlock switch 1: An interlock switch is integrated on the Transmitted Light arm and activated when the Transmitted Light arm is folded back (interlock switch opens). Interlock switch 2: An additional interlock switch is also installed in the microscope, which is activated (interlock switch opens) when the beam path to the eyepiece is released.

Compatible coupling: Manufacturer: binder Type: 99-0096-102-05 (5-pin cable socket, 711 series subminiature round plug connector) Type: 79-1414-12-05 (5-pin cable socket with 2m cable, 702 series subminiature round plug connector) Type: 99-0414-00-05 (5-pin cable socket, 712 series subminiature round plug connector) Pin assignment: Switching contact between pin 1 and pin 5. Maximum permitted electrical characteristic values of the integrated interlock switch: Umax= 40 V DC Imax= 0.2 A

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3. SAFETY NOTES

3.3.2

Screwed-on filter turret cover

As a safety precaution on laser microscope systems, the fluorescent turret disk cover is secured using 2 DIN 912 M4x6 lens head Allen screws. (Order No. 11 703 121 630 000)

3.3.3

Laser coupling by the user

Because of a danger for the user due to laser radiation, the Leica DMi8 microscopes in the standard version are not suitable without additional laser safety measures for coupling laser radiation1). If you are, however, modifying a microscope and couple a laser beam into it, as the user, you assume responsibility as the manufacturer of the complete system.

WARNING Store the screws in a safe place. Never operate the microscope when the cover is not screwed into place!

We do not make provisions for your interference with the device and, therefore, you are fully responsible for the modifications regarding the adherence to basic safety requirements and laser protective measures set forth by the European Union guidelines and by the national legal and technical directives and standards. If you modify your microscope by coupling a laser beam without authorization, when determining and implementing laser safety measures, also take note of the subsequently described hazards at the microscope, as here the danger exists of serious and irreversible eye damage and skin injuries!

WARNING

Turn the screws to secure the filter turret cover

Danger of serious and irreversible eye damage from laser radiation! Radiation of the eye or skin from direct or indirect laser radiation has to be avoided by all means! The laser light can cause serious eye damage and skin damage!

1) Laser radiation of Class 1 lasers is exempt. Further details regarding this topic in Section 3.3.4

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3. SAFETY NOTES

3.3.3.1 Safety hazard due to folded back Transmitted Light arm If you fold back the Transmitted Light arm, the laser radiation escaping from the lenses is beamed directly into the open space.

Important safety note As a safety measure, we recommend the microscope models with integrated interlock connectors as mentioned in Section 3.3.3.7.

Folded back Transmitted Light arm; the light output out of the objective is not shadowed by the condenser base.

3.3.3.2 Safety hazard at eyepiece The laser radiation that you coupled into the microscope can disperse from the eyepiece and directly hit the user's eye!

Important safety note As a safety measure, we recommend the microscope models with integrated interlock connectors as mentioned in Section 3.3.3.7.

Eyepieces

3.3.3.3 Safety hazard in the condenser area during operation. If you couple a high power laser beam into the microscope, the laser radiation, broadened by the lens, that passes the condenser laterally, can exceed the permitted limit values for eyes and skin.

Increased laser radiation can beam laterally past the condenser

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3. SAFETY NOTES

3.3.3.4 Safety hazard above the condenser The laser beam path between the condenser base and the illuminated field diaphragm is without obstruction.

Laser beam path between condenser base and illuminated field diaphragm without obstruction

3.3.3.5 Safety hazard in the specimen area The laser radiation is accessible without obstruction in the specimen area of the microscope!

Specimen area

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3. SAFETY NOTES

3.3.3.6 Safety hazard due to openings at microscope Make sure, as part of your risk assessment, that all openings at the microscope are tightly sealed and can only be opened with tools to prevent laser radiation from escaping!

Furthermore, the terminals that are not occupied by objectives have to be closed with caps at the objective nosepiece.

The openings to consider, for example: top port next to the eyepieces, left and right side port, bottom port, infinity port, connection for Transmitted Light lamp housing at the arm, lamp connection for fluorescence/connection for incident light lamp housing, cover for fluorescent turret disk. The material that is used to seal off the openings has to be suitable for the intensity of the coupled laser beam.

Openings at the stand that have to be tightly sealed a Cover for fluorescent turret disk b Transmitted Light lamp housing c Incident light lamp housing d Side port right e Bottom port f Side port, left g Objective nosepiece h Top port i Infinity port j Openings at objective nosepiece

j

b

h

a i c

g f

d e 15

3. SAFETY NOTES

3.3.3.7 Note regarding the safety hazards in Sections 3.3.3.1 and Section 3.3.3.2 Important safety note The overall responsibility for laser safety of the laser microscope system assembled by you remains with you as the responsible body, even if you use the microscopes from Leica Microsystems CMS GmbH listed below in conjunction. Within the context of your system composition and modifications, you have to verify whether the component selected by you from this chapter is effective and suitable for your laser protection concept. We recommend that for your laser applications you use a microscope variant with the following selection options because the microscope is already equipped with interlock switches at the factory: Leica No. 11889086 Laser Safety Kit and also one of the following options:

a) Safety function for the safety hazard mentioned in Section 3.3.3.1. The special microscope variants have a built-in interlock switch on the Transmitted Light arm that is activated when folding back the Transmitted Light arm (interlock switch opens). The interlock connector of the microscope must be connected to your laser beam interrupter, which interrupts the laser beam coupled into the microscope when opening the interlock switch or folding back the illumination arm. b) Safety function for the safety hazard mentioned in Section 3.3.3.2. An additional interlock switch is also installed in the microscope, which is activated (interlock switch opens) when the beam path to the eyepiece is released. The interlock switch of the microscope must be connected to your laser beam interrupter, which interrupts the laser beam coupled into the microscope when opening the interlock switch or releasing the beam path towards the eyepiece. Please note that the laser radiation may not be coupled via the bottom port!

Leica No. 11889077 Left motorized side port, 100% and interlock Leica No. 11889078 Right motorized side port, 100% and interlock Leica No. 11889079 Right/left motorized side port, 100% and interlock Leica No. 11889087 Manual side port (100/0), Int. Leica No. 11889088 Left manual side port (100/0)

Note: The fixed Transmitted Light arm (order No. 11525105 (with short cable) or order No. 11525116 (with long cable) is not suitable for laser applications and therefore is not provided for these applications.

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Bottom port

Both interlock switches a) and b) are routed to a shared interlock connector on the microscope; you have to connect your laser beam interrupter to this connector.

3. SAFETY NOTES

When doing so, note the electrical characteristic values of the integrated interlock switches in the microscope. You must not exceed these values by connecting a laser beam interrupter, as otherwise the interlock switches will be damaged and no longer function safely and reliably: Maximum permitted electrical characteristic values of the integrated interlock switch: Umax= 40 V DC Imax= 0.2 A The integrated interlock switches in the microscope and their wiring with a laser beam interrupter/laser ensure that the laser radiation you coupled into the microscope cannot get into the user's eyes through the eyepieces.

3.3.4. Exception for Class 1 lasers Coupling a laser of Class 1 in accordance with IEC/EN 60825-1 into a microscope does not result in any danger to the user. The listed safety hazards apply only for lasers of Class 1M, 2, 2M, 3R, 3B and 4. Note that the rules and regulations of the United States FDA 21CFR1040.10 Class I (CFR – Code of Federal Regulation Title 21, revised as of April 1, 2011) do not distinguish between Class 1 and 1M. Thus the statement that there are no safety hazards on the microscope do not apply for the laser classification in accordance with FDA Class 1.

Important safety note In addition, observe the safety hazards described in Section 3.3.3.3 to 3.3.3.6!

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3. SAFETY NOTES

3.5

Storage and transport

Weight

CAUTION

Transport Caution! Transport and storage at -20 °C – +85 °C and max. 90% humidity.

For shipping or transporting the microscope and its accessory components, the original packaging should be used. As a precaution to prevent damage from vibrations, the following components should be disassembled and packaged separately:

At a full load, the microscope weighs more than 18 kg. The user must take corresponding precautions for transporting the instrument.

Caution! For transport, always remove all components listed under "Transport!".

3.6

Ambient conditions

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Caution! This microscope may not be used at elevations over 2000 m above sea level.

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18

Caution! Do not use this instrument near sources of high electromagnetic radiation (for example, unshielded, intentionally operated ultra-high frequency sources), because these can disrupt proper operation. We recommend assessing the electromagnetic environment before operation of this instrument and then giving corresponding instructions.