Leica CM1850 UV Cryostat Instruction Manual V1.4 Oct 2010

INFORMATION

The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and stateof-the-art technology as we understand it following thorough investigation in this field. We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to provide our customers with additional copies, updates etc. of this manual. For erroneous statements, drawings, technical illustrations etc. contained in this manual we exclude liability as far as permissible according to the national legal system applicable in each individual case. In particular, no liability whatsoever is accepted for any financial loss or consequential damage caused by or related to compliance with statements or other information in this manual. Statements, drawings, illustrations and other information as regards contents or technical details of the present manual are not to be considered as warranted characteristics of our products.

These are determined only by the contract provisions agreed between ourselves and our customers. Leica reserves the right to change technical specifications as well as manufacturing processes without prior notice. Only in this way is it possible to continuously improve the technology and manufacturing techniques used in our products. This document is protected under copyright laws. Any copyrights of this document are retained by Leica Biosystems Nussloch GmbH. Any reproduction of text and illustrations (or of any parts thereof) by means of print, photocopy, microfiche, web cam or other methods – including any electronic systems and media – requires express prior permission in writing by Leica Biosystems Nussloch GmbH. For the instrument serial number and year of manufacture, please refer to the name plate at the back of the instrument.

© Leica Biosystems Nussloch GmbH

Leica Biosystems Nussloch GmbH Heidelberger Str. 17 - 19 D-69226 Nussloch Germany Phone: Fax: eMail: Internet:

+49 (62 24) 143-0 +49 (62 24) 143-268 [email protected] http://www.leica-microsystems.com

Leica CM1850 UV – Cryostat

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Table of contents 1.

Important Information ... 6 1.1 1.2 1.3 1.4

2.

Safety ... 7 2.1 2.1.1 2.1.2 2.2 2.3 2.4 2.5 2.6 2.7

3. 4.

Connection to mains power ... 17 Prior to operation ... 17 Leica CM1850UV - Overview ... 18 Mains switch and automatic mains fuse ... 20 Turning the instrument on ... 20

Control panel operation... 21 6.1 6.2 6.2.1 6.2.2 6.2.3 6.2.4 6.2.5 6.2.6 6.3 6.4

4

Site requirements ... 14 Transport to the desired location ... 14 Standard delivery ... 15 Handwheel assembly ... 16

Setup ... 17 5.1 5.2 5.3 5.4 5.5

6.

Safety features ... 7 Locking the handwheel ... 7 Knife guard ... 8 General information on instrument design and safe handling ... 8 Operating conditions ... 9 Operating the instrument ... 10 Cleaning and disinfection ... 10 Removal of the microtome ... 11 Maintenance ... 11

Technical data ... 12 Unpacking and installation ... 14 4.1 4.2 4.3 4.4

5.

Symbols used in this manual and their meaning ... 6 Qualification of personnel ... 6 Designated use ... 6 Instrument type: ... 6

Control panel 1 ... 21 Programming the desired values ... 22 Setting the time ... 22 Setting the automatic defrost time (cryochamber) ... 22 Selecting the cryochamber temperature ... 22 Activation of the Peltier element (option) ... 23 Manual defrosting of the quick freeze shelf ... 23 Manual defrosting of the cryochamber ... 24 Display lock ... 24 Control panel 2 - Electric coarse feed ... 25

Instruction Manual V 1.4 – 10/2010

Table of contents 7.

Daily use of the instrument ... 26 7.1 7.1.1 7.1.2 7.2 7.2.1 7.2.2 7.3 7.3.1 7.3.2 7.4 7.5 7.5.1 7.5.2 7.5.3 7.6 7.6.1 7.6.2

8.

Troubleshooting ... 33 8.1 8.2 8.3

9.

Cleaning ... 39 Spray disinfection with Leica Cryofect ... 40 Maintenance ... 41 General maintenance ... 41 Removal of the microtome ... 42 Removal of the microtome cover ... 44 Reinstallation of the microtome ... 44 Replacement of UVC lamp ... 46 Replacement of the fuses ... 46 Replacement of the lamp ... 48

Ordering information, optional accessories ... 49 10.1 10.2 10.2.1 10.2.2

11. 12. 13.

Error messages in the display ... 33 Temperature control button ... 33 Possible causes and remedies ... 34

Cleaning, disinfection, maintenance ... 39 9.1 9.2 9.3 9.3.1 9.3.2 9.3.3 9.3.4 9.3.6 9.3.5 9.3.7

10.

Specimen freezing ... 26 Quick freeze shelf ... 26 Stationary heat extractor ... 26 Specimen discs ... 27 Inserting the specimen discs in the specimen head ... 27 Specimen orientation ... 27 Sectioning ... 28 Trimming the specimen ... 28 Section thickness setting ... 28 Temperature selection chart (in minus °C) ... 29 Defrosting ... 30 Automatic defrosting of the cryochamber ... 30 Manual defrosting of the cryochamber ... 30 Manual defrosting of the quick freeze shelf ... 31 Terminating work ... 31 Terminating daily work ... 31 Shutdown for a longer period ... 32

Ordering information ... 49 Optional accessories ... 51 Mobile heat extractor ... 51 Thermal block ... 51

Warranty and service ... 52 Decontamination Certificate (master) ... 53 Information for the People´s Republik of China ... 55

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1.

Important Information

1.1

Symbols used in this manual and their meaning Warnings appear in a grey box and are marked by a warning triangle . Notes i.e. important user information appears in a grey box and is marked by an information symbol.

Caution – UVC radiation!

(5) (Fig.5)

1.2

Figures in brackets refer to item numbers in drawings or to the drawings themselves.

Qualification of personnel

The Leica CM1850 UV may only be operated by trained laboratory personnel. All laboratory personnel designated to operate the instrument must carefully read the present instruction manual prior to starting work with the instrument.

1.3

Designated use

The Leica CM1850 UV is a powerful cryostat for routine as well as research applications in biology, medicine and industry. The instrument has been designed for rapid freezing and sectioning of tissue samples and is equipped with an automated disinfection system. The instrument is not suitable for unattended storage of tissue material. The instrument may only be operated within the scope of its designated use as described above and as per the instructions given in this manual. The Leica CM1850 UV is also suitable for IVD (in vitro diagnostics).

1.4

Instrument type:

All information given in this instruction manual applies only to the instrument type indicated on the title page. A name plate, indicating the instrument serial number, is attached to the back of the instrument. Fig. 1

Despite chemical and/or UV-light disinfection, adequate safety measures as per the applicable laboratory regulations must still be taken (i.e. safety goggles, gloves, laboratory coat and mask must be worn). This type of disinfection reduces the number of germs by at least 99.9 %.

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Instruction Manual V 1.4 – 10/2010

2.

Safety

This instruction manual includes important instructions and information related to the operating safety and maintenance of the instrument. The instruction manual is an important part of the product which must be read carefully before setting up and using the instrument and must always be kept with the instrument. If additional requirements are imposed by regulations and/or laws on accident prevention and environmental protection in the country of operation, appropriate instructions for compliance with such requirements must be added to this manual.

2.1

Safety features The instrument incorporates the following safety features: safety handwheel and knife guards on the knife holders. To prevent adverse health effects from UV radiation, the UV disinfection cycle can be started only after the sliding window has been properly closed. Closing the window activates the corresponding safety features.

The consistent use of these safety features and strict observation of the warnings and cautions in this manual, will safeguard the operator from accidents and/or personal injury to a great extent.

2.1.1 Locking the handwheel 2 1

Fig. 2

Prior to manipulating the knife and specimen, or changing the specimen or knife, and during breaks, always lock the handwheel!

For locking the handwheel rotate the handle until it is in the upper position. Push the locking pin (1) into the recess at the handwheel. The locking position is marked by a black dot (2). If necessary, move the handwheel slightly forth and back until the locking mechanism engages. To unlock, push the locking pin (1) to the left from the recess at the handwheel. Only rotate the handwheel when the refrigeration system is on and the cryochamber is cold.

Fig. 3

Leica CM1850 UV – Cryostat

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2.

Safety

2.1.2 Knife guard For every manipulation in the cryochamber, or upon changing a specimen when the knife or disposable blade is clamped, or during breaks, cover the cutting edge with the knife guard.

The knife holders CN and CS are equipped with knife guards; on the knife holder CE the glass plate of the anti-roll guide constitutes the knife guard (please refer to the separate instruction manual for your knife holder). Prior to manipulating the knife and specimen, or changing the specimen or knife, and during breaks, always lock the handwheel!

2.2

General information on instrument design and safe handling This instrument has been built and tested in accordance with the following safety regulations on electrical measuring, control, regulating and laboratory devices. For current information about applicable standards, please refer to the CE declaration of conformity on our Internet site: www.leica-microsystems.com In order to maintain this condition and to ensure safe operation, the operator must observe the instructions and warnings contained in this instruction manual.

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Instruction Manual V 1.4 – 10/2010

2. Safety 2.3

Operating conditions Transport and installation • After transporting do not turn the instrument on for a minimum of 4 hours! • Do not operate in rooms with explosion hazard! • To ensure an adequate cooling capacity, the instrument must be set up with at least 10 cm distance from walls and furniture!

Connection to mains • Before connecting to the mains power, please check if the local voltage complies with the power rating specified on the name plate of the instrument! • During the compressor start-up the nominal voltage must not drop below the values specified in the ‘Technical data‘! The compressor requires a start-up current between 45 and 50 A. Therefore, the electric circuit at the place of installation must be inspected by an electrical engineer to ensure that it meets the requirements for a smooth operation of the instrument. A constant adequate power supply to the instrument must be ensured at all times. Failure to comply with the above will cause severe damage to the instrument. • After transporting, wait at least 4 hours before turning the instrument on. This waiting period is necessary to allow the compressor oil, which may have been displaced during transport, to return to its original position. Failure to comply with this can cause severe damage to the instrument.

Defrosting • The quick freeze shelf may become hot during defrosting! Therefore, do not touch it!

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2.

Safety

2.4

Operating the instrument • Be very careful when handling microtome knives and disposable blades. The cutting edge is extremely sharp and can cause severe injury! • Never leave knives and knife holders with a knife/blade mounted lying around! • Never place a knife on a table with the cutting edge facing upward! • Never try to catch a falling knife! • Always clamp the specimen before the knife! • Prior to manipulating the knife and specimen, or changing the specimen or knife, and during breaks, always lock the handwheel and cover the cutting edge with the knife guard! • Avoid skin contact with cold parts of the instrument as this can cause frostbite! • To make sure that the condensation water stemming from the defrost cycles drains into the waste container and to avoid the risk of possible contamination, ensure that the tap of the waste container (2, Fig. 22) is open when operating the instrument. Only shut the tap when draining the waste container!

2.5

Cleaning and disinfection • It is not necessary to remove the microtome for routinely disinfecting the cryochamber. The instrument has been designed for UV disinfection. Spray disinfection with Leica Cryofect is also possible, thanks to the special insulation of the microtome. (Cryofect is not available in all countries!) Remove section waste EVERY TIME after changing the specimen! Erst dann Desinfektion starten! Each new specimen is a potential source of contamination.

• Do not use organic solvents or any other aggressive substances for cleaning and disinfection! Only use the cleaning agents and disinfectants specified in this instruction manual such as Leica Cryofect (alcohol or common disinfectants based on alcohol)! Please contact Leica Biosystems for further details on adequate disinfection measures.

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Instruction Manual V 1.4 – 10/2010

2. Safety 2.6

Removal of the microtome • Prior to removing the microtome, turn the instrument off with the mains switch and pull the mains plug. • Prior to removing the microtome, bring the specimen head to the lower position with the handwheel. Otherwise the specimen head would rapidly fall down and might injure the operator‘s hands, when taking out the microtome. • Wear appropriate protective gloves to take the cold microtome out of the cryochamber. • Extended skin contact with cold instrument parts - risk of frost bite! • The microtome must be entirely dry before reinstallation. Humidity inside will condense and freeze in the cold cryostat and thus cause malfunctions or damage. • Do not use external heaters for drying the cryochamber. This can cause damage to the cooling system! • All components removed from the cryostat must be carefully dried before they are put back into the cryochamber

2.7

Maintenance Replacement of the fuses • Turn the instrument off with the automatic mains fuse and pull the mains plug before replacing the fuses. • Only use fuses of the same specification! For the required values, please refer to Chapter 3 ‘Technical data‘. Using fuses other than those specified by the manufacturer may cause severe damage to the instrument!

Replacement of the lamp • Turn the instrument off with the automatic mains fuse and pull the mains plug, before replacing the lamp. When removing / installing the microtome improperly or when changing the UVC lamp, the lamp can be destroyed. If this happens, the lamp change must be completed by Technical Service. If any metallic mercury is released, handle it carefully and dispose of it properly. If both disinfection indicator lights are blinking alternately, the UV tube must be replaced!

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3.

Technical data Operating temperature range (ambient temperature): 18 °C to 35 °C. All specifications related to temperature are valid only up to an ambient temperature of 22 °C and an air humidity lower than 60 %!

Type Mark of conformity Nominal voltage Nominal frequency Power input Max. start-up current for 5 sec Protective class Automatic mains fuse Pollution degree Overvoltage inst. category Heat emission (max.)

CM1850 UV 100 V AC ±10% 50/60 Hz 1440 VA 45 A eff. I T12A T1 2 II 1600 J/s

CM1850 UV CUL 120 V AC ±10% 60 Hz 1440 VA 45 A eff. I T12A T1 2 II 1600 J/s

CM1850 UV 220 V AC ±10% 60 Hz 1440 VA 45 A eff. I T12A T1 2 II 1600 J/s

CM1850 UV CM1850 UV VDE 230 V AC ±10% 240 V AC ±10% 50 Hz 50 Hz 1440 VA 1440 VA 45 A eff. 45 A eff. I I T10A T1 T10A T1 2 2 II II 1600 J/s 1600 J/s

according to IEC-1010, UL 3101

Refrigeration system Cryochamber Temperature setting range Defrosting Refrigeration capacity Safety factor Refrigerant Compressor oil Quick-freeze shelf Max. temperature No. of quick-freeze stations Defrosting Peltier element (optional) Lowest possible temperature No. of quick-freeze stations Defrosting

50 Hz

60 Hz

0 °C to -35 °C (+ 2 K/- 0 K) automatic hot gas defrosting 1 automatic defrost cycle/24 hrs, temperature controlled; 690 W 3 300g ±5g refrigerant R 404A * 0.6 l EMKARATE RL-22S, ICI *

0 °C to -35 °C (+2 K/- 0 K) automatic hot gas defrosting 1 automatic defrost cycle/24 hrs, temperature controlled; 690 W 3 300 g ±5g refrigerant R 404A* 0.6 l EMKARATE RL-22S, ICI *

- 40 °C (+ 0 K/- 2 K) 10 manual hot gas defrosting, time controlled

- 40 °C (+ 0 K/- 2 K) 10 manual hot gas defrosting, time controlled

-60 °C (+5K) 2 Together with quick-freeze shelf

-60 °C (+5K) 2 Together with quick-freeze shelf

according to CECOMAF Liquid temperature 45 °C, evaporation temperature -25 °C

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Instruction Manual V 1.4 – 10/2010

3.

Technical data

* Refrigerant and compressor oil must only be replaced by qualified, authorized service personnel!

Microtome Rotary microtome Section thickness setting 1 - 60 µm Total specimen feed 25 mm Vertical stroke 59 mm Maximum specimen size 55 x 55 mm Specimen orientation 8° (x-, y-, z-axis) Electric coarse feed 200 µm/s slow rapid 700 µm/s

Lamp 50 Hz version: 60 Hz version:

Osram Dulux S 11 W/21 Color: LUMILUX light white Osram Dulux S 13 W/21 Color: LUMILUX light white

Cryocabinet Dimensions Width (w/o handwheel) Width (with handwheel) Depth Height

600 mm 730 mm 730 mm 1140 mm

Please refer to section 4.1 ‘Site requirements‘!

Storage conditions Weight (incl. microtome, without specimen cooling)

ca.135 kg

Temperature: 5 - 55 °C Relative humidity: < 85 %, non-condensing

All CM 1850 UV instrument types require the following secondary fuses: F1: T0.25 A Fa. Schurter, Type FST; 6.3x32 mm or T0.25 A Fa. Littlefuse, Type 313; 6.3x32 mm F2: T0.6 A Fa. Schurter, Type FST; 6.3x32 mm or T0.6 A Fa. Littlefuse, Type 313; 6.3x32 mm F3: T1.6 A Fa. Schurter, Type FST; 6.3x32 mm or T1.6 A Fa. Littlefuse, Type 313; 6.3x32 mm F4: T6.25 A Fa. Schurter, Type FST; 6.3x32 mm or T6.25 A Fa. Littlefuse, Type 313; 6.3x32 mm F5: T4 A Fa. Schurter, Type FST; 6.3x32 mm or T4 Fa. Littlefuse, Type 313; 6.3x32 mm Leica CM1850 UV – Cryostat

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4.

Unpacking and installation

4.1

Site requirements Do not operate in rooms with explosion hazard! To ensure an adequate cooling capacity, the instrument must be set up with at least 10 cm distance from walls and furniture.

• The place of installation must meet the following requirements: – no direct sunlight, – mains power socket at a distance no greater than approximately 1.5 m, – no drafts (air condition outlets, etc.) directly over the instrument, – even floor, – mainly vibration-free floor, – obstruction-free access to the handwheel, – room temperature max. 35 °C, – Air humidity must not exceed 60 %. – The instrument is designed for indoor use only. High room temperatures and excessive air humidity affect the cooling capacity of the cryostat.

4.2

Transport to the desired location

16

Lengthwise movement

15 Fig. 4

14

• Move the instrument to the installation site on its wheels (14). Please note the areas which are reinforced for transporting and grip the cabinet only at these locations (Fig. 4). Tilt • The adjustable feet (15) can support the weight of the instrument when tipping at a slight angle (max. 30 °). Sideways • At the installation site unscrew the screws in the adjustable feet (15) movement using a fork wrench. This is necessary to ensure stability. • Align the adjustable feet to level the instrument. 14

The instrument must be transported in an upright position only. When the instrument is tilted, the compressor oil is displaced. Do not grip the cabinet at the lid. Grip the cabinet only at the marked locations ( ). The alignment of the adjustable feet is necessary to ensure an unobstructed drain of the quick freeze shelf defrosting water.

Instruction Manual V 1.4 – 10/2010

4. Unpacking and installation 4.3

Standard delivery

It gives following variations: 1 1 1 1 1

1 1 1 1 1

1 1 1

- CM1850UV with Retraction, in 4 different voltages - CM1850UV without Retraction (only US) ... 14 0471 31148 knob with marking, antibacterial ... 14 0471 42558 Heat extractor, stationary ... 14 0471 30792 Low temperature stabilizer for heat extractor, (Parking station)... 14 0471 30793 Optional clamping lever for object head ... 14 0112 28806 Set of specimen discs ... 14 0470 43550 - 4 specimen discs, 25 mm ... 14 0416 19275 - 4 specimen discs, 30 mm ... 14 0370 08587 Section waste tray ... 14 0471 30787 Storage shelf, right ... 14 0471 30789 Storage shelf, left ... 14 0471 30790 Brush shelf ... 14 0398 13088 toolkit: ... 14 0436 43463 - 1 brush, fine ... 14 0183 28642 - 1 “Leica” brush w/magnet ... 14 0183 40426 - 1 Allen key, no. 1.5 ... 14 0222 10050 - 1 Allen key, no. 2.5 ... 14 0222 04137 - 1 Allen key, no. 3.0 ... 14 0222 04138 - 1 Allen key, no. 4.0 ... 14 0222 04139 - 1 Allen key with spherical head, no. 4.0 ... 14 0222 32131 - 1 Allen key, no. 5 ... 14 0222 04140 - 1 Allen key with handle, no. 5 ... 14 0194 04760 - 1 Allen key, no. 6 ... 14 0022 04141 - 1 Single-head wrench, no. 13 ... 14 0330 33149 - 1 Single-head wrench, no. 16 ... 14 0330 18595 Bottle of cryostat oil, type 407, 50 ml ... 14 0336 06098 Bottle of OCT-Compound, mounting medium for cryosectioning, 125 ml ... 14 0201 08926 Instruction manual Leica CM1850UV (+ language CD) ... 14 0471 80002 Compare the delivered components with the parts list and your order. Should you find any discrepancies, please contact your Leica sales office without delay. A choice of different knife holders is available for the CM 1850UV. The knife holder is accompanied by its own separate instruction manual. Please contact your Leica sales office if the instruction manual is missing.

Leica CM1850 UV – Cryostat

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4.

Unpacking and installation

4.4

Handwheel assembly 1

2

3 4

• Insert the pin (1) of the handwheel shaft in to the hole (2) of the handwheel. 5

• Mount the spring washer (3) on the screw (4) as shown in Fig. 16. • Tighten the screw (4) with an Allen key (5 mm). • Attach the cover disc (5) (self-adhesive). To dismount, proceed in reverse order.

Fig. 5

The handwheel including the fixing components are packed in the cardboard box for the accessories. The handwheel can be dismounted for transporting (e.g. narrow doors).

Only rotate the handwheel when the refrigeration system is on and the cryochamber is cold.

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Instruction Manual V 1.4 – 10/2010

5. 5.1

Setup

Connection to mains power During the start-up of the compressor the nominal voltage must not drop below the values specified in the ‘Technical data‘. Please note that the compressor requires a start-up current between 45 and 50 A. Therefore, the electric circuit at the installation site must be inspected by an electrical engineer to ensure that it meets the requirements for a smooth operation of the instrument. A constant adequate power supply to the instrument must be ensured at all times. Failure to comply with the above will cause severe damage to the instrument.

5.2

•

The electric circuit at the place of installation must be protected separately.

•

Do not connect any other consumers to this electric circuit.

Prior to operation

Leica CM1850 UV – Cryostat

•

Before connecting the instrument to the mains power, please check if the local mains voltage complies with the power rating indicated on the name plate of the instrument.

•

Place the storage shelves in the cryochamber.

•

Place the section waste tray and brush shelf in the cryochamber.

•

Place the knife holder base on the microtome base plate.

•

Insert the knife holder and clamp it on the base plate For details, please refer to the separate instruction manual for your knife holder).

•

Open the knife box with the knife and place it in the cryochamber for precooling.

•

Place all tools needed for specimen preparation in the cryochamber.

•

Close the sliding window.

•

Connect the mains plug to the mains power outlet at the wall.

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5.

Setup

5.3

Leica CM1850 UV - Overview 17 15 4

13

16

21

20

8

13

13 19

12

10 18 Fig. 6

18

14 9

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Instruction Manual V 1.4 – 10/2010

5.

Setup

3 6

5

1 2 3 4 5 6 7 8 7

2

Leica CM1850 UV – Cryostat

1

9 10 11 12 13 14 15 16 17 18 19 20 21

Cryostat CM1850 UV Waste container Control panel 1 Control panel 2 Storage shelf, left Storage shelf, right Automatic mains fuse Support for stationary heat extractor Quick freeze shelf Peltier element Parking station Stationary heat extractor Specimen disc Thermal bloc (accessory) Section waste tray Brush shelf Orientable specimen head Knife holder base (accessory) Knife holder CE (accessory) Knife holder CN (accessory) Knife holder CE-TC (accessory)

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5.

Setup

5.4

Mains switch and automatic mains fuse The automatic mains fuse is used as mains switch. To turn the automatic mains fuse on, the switch must be set in the upper position (pos. 1). To turn the automatic mains fuse off, the switch must be set in the lower position (pos. 0).

Fig. 7 Automatic mains fuse

5.5

Turning the instrument on After transporting, wait at least 4 hours before turning the instrument on. This waiting period is necessary to allow the compressor oil, which may have been displaced during transport, to return to its original position. Failure to comply with this can cause severe damage to the instrument.

• Turn the instrument on with the automatic mains fuse. • The instrument has been configured ex works as follows: Time: Defrost time: Cryochamber cooling: Peltier element (option):

00:00 10:00 On (Indication of the temperature) Off Indication ‘PE‘

• Set the desired values as described in the sections 6.2.1 to 6.2.3. During normal operation pressure compensation prior to the compressor start-up might lead to a hissing sound.

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Instruction Manual V 1.4 – 10/2010

6. Control panel operation 6.1

Control panel 1

Fig. 8

Function keys Lamp button ON/OFF switch for cryochamber illumination. Manual defrost button To activate and deactivate manual defrosting. Key button To lock and unlock the control panel to protect the entered parameters from unintended modifications. To lock or unlock, hold down for approximately 5 seconds. Disinfection Duration - 30 min Duration - 180 min

UVC key - to activate / deactivate the disinfection cycle and/or to acknowledge interruption of a disinfection cycle. To start disinfection the sliding window must be completely closed. Press UVC key once briefly to start the 30 min mode Press UVC key once for a longer time (approx. 4 sec) to start the 180 min mode Opening the sliding window interrupts the disinfection cycle. Press the UVC key to resume the disinfection cycle!

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