Leica CM1860 and CM1860UV Instructions for Use Rev D V1.3 Sept 2012

NOTE The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and state-of-the-art technology as we understand it following thorough investigation in this field. We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to provide our customers with additional copies, updates etc. of this manual. To the extent permitted in accordance with the national legal system as applicable in each individual case, we shall not be held liable for erroneous statements, drawings, technical illustrations etc. contained in this manual. In particular, no liability whatsoever is accepted for any financial loss or consequential damage caused by or related to compliance with statements or other information in this manual. Statements, drawings, illustrations and other information regarding the contents or technical details of the present Instructions for Use are not to be considered warranted characteristics of our products. These are determined only by the

contract provisions agreed between ourselves and our customers. Leica reserves the right to change technical specifications as well as manufacturing processes without prior notice. Only in this way is it possible to continuously improve the technology and manufacturing techniques used in our products. This document is protected under copyright laws. All copyrights to this documentation are held by Leica Biosystems Nussloch GmbH. Any reproduction of text and illustrations (or of any parts thereof) by means of print, photocopy, microfiche, web cam or other methods – including any electronic systems and media – requires express prior permission in writing by Leica Biosystems Nussloch GmbH. For the instrument serial number and year of manufacture, please refer to the nameplate on the back of the instrument. © Leica Biosystems Nussloch GmbH

Published by: Leica Biosystems Nussloch GmbH Heidelberger Str. 17 - 19 D-69226 Nussloch Germany Phone: +49 62 24 143-0 Fax: +49 6224 143-268 Internet: http://www.leica-microsystems.com Leica CM1860/CM1860 UV – Cryostat

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Table of Contents 1.

Important Information... 6 1.1 1.2 1.3 1.4

2.

Safety... 9 2.1 2.2 2.3 2.3.1 2.3.2 2.4 2.5 2.6 2.7 2.8

3. 4.

Electrical connection... 24 Prior to operation... 24 Overview of the Leica CM1860/CM1860 UV... 25 Power switch and circuit breaker... 27 Switching on the instrument... 27

Operating the Instrument... 28 6.1 6.2 6.2.1 6.2.2 6.2.3 6.2.4 6.2.5 6.2.6 6.2.7 6.3 6.4 6.5

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Installation site requirements... 18 Unpacking instructions for the cryostat CM1860/1860 UV... 19 Standard delivery-packing list... 22 Assembling the handwheel... 23

Instrument Setup... 24 5.1 5.2 5.3 5.4 5.5

6.

Safety notes... 9 Warnings... 9 Safety devices... 10 Locking/unlocking the handwheel... 12 Knife guard... 12 Operating conditions... 13 Operating the instrument... 14 Cleaning and disinfection... 14 Removing the microtome... 15 Maintenance... 15

Technical Data... 16 Setting up the Instrument... 18 4.1 4.2 4.3 4.4

5.

Symbols in the text and their meanings...6 Qualification of personnel... 8 Intended use of instrument... 8 Instrument type... 8

Control panel 1... 28 Programming the desired values... 31 Setting the time... 31 Setting the automatic defrost time (cryochamber)... 31 Selecting the cryochamber temperature... 31 Activation of the Peltier element (option)... 32 Manual defrosting of the quick freeze shelf... 32 Manual defrosting of the cryochamber... 33 Setting the section thickness... 33 Retraction (factory default setting: disabled)... 33 Display lock... 34 Control panel 2 - Electric coarse feed... 34 Instructions for Use V 1.3 – 09/2012

7.

Daily Use of the Instrument... 35 7.1 Specimen freezing... 35 7.1.1 Quick freeze shelf... 35 7.1.2 Stationary heat extractor (optional)... 36 7.2 Specimen discs... 36 7.2.2 Specimen orientation... 36 7.2.1 Inserting the specimen discs into the specimen head... 36 7.3 Sectioning... 37 7.3.1 Blade holder CE... 37 7.3.2 Blade holder CE-TC... 43 7.3.3 Knife holder CN... 44 7.3.4 Knife holder CN-Z with anti-roll guide... 48 7.3.5 Cleaning the blade and knife holders... 48 7.3.6 Installing the shelf (movable) (optional)... 50 7.4 Temperature selection chart (in minus °C)... 51 7.5 Defrosting... 52 7.5.1 Automatic defrosting of the cryochamber... 52 7.5.2 Manual defrosting of the cryochamber... 52 7.5.3 Manual defrosting of the quick freeze shelf... 53 7.6 Terminating work... 53 7.6.1 Terminating daily work... 53 7.6.2 Shutdown for a longer period... 54

8.

Troubleshooting... 55 8.1 8.2 8.3 8.3.1

9.

Cleaning, Disinfection, Maintenance... 62 9.1 9.2 9.3 9.3.1 9.3.2 9.3.3 9.3.4

10.

Cleaning... 62 Spray disinfection with Leica Cryofect... 63 Maintenance... 64 General maintenance instructions... 64 Replacing the fuses... 66 Replacing the UVC lamp... 66 Replacing the fluorescent lamp... 68

Ordering Information, Components and Consumables... 69 10.1 10.2 10.3

11. 12.

Error messages in the display... 55 Temperature control button... 56 Possible sources of error, causes and remedies... 57 Changing the battery... 61

Ordering information... 69 Mobile heat extractor – application... 94 Cold extractor – application... 94

Warranty and Service... 95 Decontamination Certificate (Master)... 96

Leica CM1860 /CM1860UV – Cryostat

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1.

Important Information

1.1

Symbols in the text and their meanings Warnings appear in a gray box and are marked . by a warning triangle Useful tips, i.e. important information for the user, appear in gray boxes and are marked by an . Caution – UVC radiation!

(5) (Fig.5)

Numbers in parentheses refer to item numbers in illustrations or to the illustrations themselves.

Manufacturer This product fulfills the requirements of the Council's Directive 98/79/EC concerning in vitro diagnostics (IVD) medical devices. In vitro diagnostics (IVD) medical device Observe the Instructions for Use REF

Order No.

SN

Serial number

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Symbol for labeling electrical and electronic equipment in accordance with Section 7 of the German Electrical and Electronic Equipment Act (ElektroG). ElektroG is the law governing how electrical and electronic equipment is brought into circulation, returned and disposed of in an environmentally friendly manner. The CSA test mark means that a product has been tested and fulfills the applicable safety and/or performance standards, including the relevant standards defined or administered by the American National Standards Institute (ANSI), Underwriters Laboratories (UL), the Canadian Standards Association (CSA), the National Sanitation Foundation International (NSF) and others. Environmental protection symbol of the China RoHS directive. The number in the symbol indicates the "Environmentfriendly Use Period" of the product. The symbol is used if a substance restricted in China is used in excess of the maximum permitted limit.

Instructions for Use V 1.3 – 09/2012

1.   Important Information The package contents are fragile and must be handled with care.

Example of labeling in accordance with IPPC

Indicates the correct upright position of the package.

• IPPC symbol • Country code in accordance with ISO 3166, e.g. DE for Germany • Regional identifier, e.g. HE for Hessen • Producer/treatment provider code, unique assigned number starting with 49 • Treatment code, e.g. HT (heat treatment), MB (methyl bromide), and possibly DB (debarked) Package labeling in accordance with German Hazardous Freight Ordinance Road and Rail (GGVSE)/European Agreement concerning the International Carriage of Dangerous Goods by Road (ADR) for transporting hazardous goods. Class 3: "FLAMMABLE LIQUID" Tip-n-Tell indicator to monitor whether the shipment has been transported and stored in upright position according to your requirements. With a pitch of 60 ° or more, the blue quartz sand flows into the arrow-shaped indicator window and sticks there permanently. Improper handling of the shipment is immediately detectable and can be proven definitively.

The package must be kept in a dry environment. It is not permitted to stack packages, and no loads may be placed on top of the package. Indicates the temperature range permitted for storing and handling the package. Minimum +5 °C Maximum +50 °C

Indicates the temperature range permitted for transporting the package. Minimum -40 °C Maximum +55 °C

Leica CM1860/CM1860 UV – Cryostat

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1.

Important Information

1.2

Qualification of personnel

Only trained laboratory personnel may operate the Leica CM1860/CM1860 UV. Prior to operating the instrument, the operator must thoroughly read and understand these Instructions for Use and must familiarize him/herself with all technical details of the instrument. Despite chemical and/or UV-light disinfection, adequate safety measures as per the applicable laboratory regulations must still be taken (i.e. safety goggles, gloves, laboratory coat and mask must be worn). These disinfections mean a reduction in the number of germs of at least 99.99 % (see certificate on the Leica website).

1.3 Intended use of instrument The Leica CM1860/CM1860UV is a high-performance cryostat with disinfection system for routine and research applications in biology, medicine and industry. The instrument is used to freeze and section specimen material quickly. The instrument is not designed for unsupervised storage of specimen material. The instrument may only be operated within the scope of its designated use as described above and as per the instructions given in these Instructions for Use. The Leica CM1860/CM1860UV is also suitable for IVD (in vitro diagnostics). Any other use of the instrument is considered improper.

1.4

Instrument type

All information contained in these Instructions for Use applies exclusively to the instrument type indicated on the title page. A nameplate bearing the instrument serial number (SN) is attached to the back of the instrument. These Instructions for Use include important instructions and information related to the operating safety and maintenance of the instrument. The Instructions for Use are an important part of the product, and must be read carefully prior to startup and use and must always be kept near the instrument. If additional requirements on accident prevention and environmental protection apply in the country of operation, these Instructions for Use must be supplemented by appropriate instructions to ensure

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compliance with such requirements. Proper and intended use includes the observance of all instructions in the Instructions for Use and compliance with all inspection and maintenance instructions.

Fig. 1

The nameplate shown here is intended as an example only!

Instructions for Use V 1.3 – 09/2012

2.   Safety The safety and caution notes in this chapter must be observed at all times. Be sure to read these notes even if you are already familiar with the operation and use of other Leica products.

2.1

Safety notes

These Instructions for Use include important instructions and information related to the operating safety and maintenance of the instrument. The Instructions for Use are an important part of the product, and must be read carefully prior to startup and use and must always be kept near the instrument. This instrument has been built and tested in accordance with the safety requirements for electrical equipment for measurement, control, and laboratory use. Residual risks The instrument has been designed and constructed with the latest state-of-the-art technology and

according to recognized standards and regulations with regard to safety technology. Operating or handling the instrument incorrectly can place the user or other personnel at risk of injury or can cause damage to the instrument or other property. The machine may be used only as intended and only if all of its safety features are in proper working condition. Malfunctions that impede safety must be remedied immediately. To maintain this condition and ensure safe operation, the user must observe all notes and warnings contained in these Instructions for Use. Only original spare parts and permitted original accessories may be used. For the CE certificate and up-todate certificates pertaining to UV disinfection, visit us online at: www.leica-microsystems.com

These Instructions for Use must be appropriately supplemented as required by the existing regulations on accident prevention and environmental safety in the operator's country. The protective devices on both instrument and components must neither be removed nor modified. Only service personnel qualified by Leica may repair the instrument and access the instrument's internal components.

2.2

Warnings The safety devices installed in this instrument by the manufacturer only constitute the basis for accident prevention. Operating the instrument safely is, above all, the responsibility of the owner, as well as the designated personnel who operate, service or repair the instrument. To ensure trouble-free operation of the instrument, make sure to comply with the following instructions and warnings.

Leica CM1860/CM1860 UV – Cryostat

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2.

Safety

2.3

Safety devices The instrument is equipped with the following safety devices: Handwheel lock and knife guard on the knife holder. To prevent adverse health effects from UV radiation, the UV disinfection cycle can be started only after the sliding window has been properly closed. Closing the window activates the corresponding safety features.

The consistent use of these safety features and strict observation of the warnings and cautions in these Instructions for Use will safeguard the operator from accidents and/or personal injury to a great extent. Personal safety precautions When working with cryostats, personal safety precautions must always be taken. It is mandatory to wear work safety shoes, safety gloves, a mask and safety goggles.

UVC disinfection is effective when disinfecting surfaces and air within the irradiated working area of the Leica CM1850UV, CM1860UV and CM1950 cryostats at -20 °C ­ (­Table 1, see I. Maier certificate). For powerful disinfection, we recommend irradiation for three hours (CM1850UV/CM1860UV/ CM1950). Vegetative bacteria including Mycobacterium tuberculosis, bacterial endospores (Bacillus sp.) and fungi are killed during this time. Even viruses including such resistant species as hepatitis are inactivated up to at least 4 log10 units (99.99 %).

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Instructions for Use V 1.3 – 09/2012

2.   Safety Personal safety precautions (continued) Medium disinfection can be attained through short irradiation for 30 minutes (CM1850UV/CM1860UV/CM1950). This reduces vegetative bacteria including Mycobacterium tuberculosis and sensitive viruses such as the influenza A virus (also including the highly pathogenic avian influenza virus H5N1) and the poliovirus by at least 5 Log 10 units (99.999 %). UVC irradiation within the working area of the cryostats can provide reliable and efficient disinfection of surfaces and the air and significantly reduces the risk of infection. We recommend removing tools and debris and then wiping off visible contamination in the cryostat with an alcohol-based disinfectant prior to using the UV lamps. The germicidal effect of the irradiation is restricted to the directly irradiated areas, which is why UVC irradiation cannot be a replacement for regular chemical disinfection of the cryostat chamber. For current information about certificates and recommendations, visit: http://www.leica-microsystems.com/products/total-histology/ cryosectioning/details/product/leica-CM1860-uv/downloads/

Leica CM1860 /CM1860UV – Cryostat

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2.

Safety

2.3.1 Locking/unlocking the handwheel 12 o'clock position

6 o'clock position Fig. 2 1 2

Rotate the handwheel only if the refrigeration system is on and the cryochamber is cold. Always lock the handwheel prior to making modifications to the knife or specimen, changing the specimen, or taking a break.

To lock the handwheel, move its handle to the 12 or 6 o'clock position. Press the lever (1) fully outward; gently rock the handwheel back and forth until the locking mechanism clicks into place noticeably. To release the handwheel, press the lever (2) on the handwheel toward the cryostat housing.

Fig. 3

2.3.2 Knife guard Prior to manipulating the knife and specimen, changing the specimen or knife, or taking a break, always lock the handwheel and cover the cutting edge with the knife guard.

The CN and CN-Z knife holders feature knife guards; the glass anti-roll plate of the CE and CE-TC blade holders also serves as a knife guard.

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Instructions for Use V 1.3 – 09/2012

2.   Safety 2.4

Operating conditions Transport and installation • After transporting, do not turn the instrument on for a minimum of 4 hours. • Do not operate in rooms with explosion hazard! • To ensure proper function, the instrument must be set up with the following minimum clearance to walls and fixtures on all sides: - rear: 15 cm - 5.9 inch - right: 30 cm - 11.8 inch - left: 15 cm - 5.9 inch Electrical connection • Before connecting the instrument to an AC power outlet, ensure that the AC power of your laboratory matches the values on the nameplate of the instrument. • During the start-up of the refrigeration system, the minimum nominal voltage must not drop below the values specified in Chapter 3, "Technical Data". The compressor draws a starting current between 45 and 50 A. Therefore, the electric circuit at the place of installation must be inspected by an electrical engineer to ensure that it meets the requirements for a smooth operation of the instrument. A stable power supply in accordance with the instrument's specifications is essential to its proper functioning. Failure to comply with the above can damage the instrument. • This waiting period is necessary to allow the compressor oil, which may have been displaced during transport, to return to its original position. Failure to comply with this may cause damage to the instrument. • No additional extension cable may be used. Defrosting • The quick freeze shelf can become very hot during the defrosting process. Therefore, do not touch it!

Leica CM1860 /CM1860UV – Cryostat

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2.

Safety

2.5

Operating the instrument • Take care when handling microtome knives and disposable blades. The cutting edge is extremely sharp and can cause serious injuries! We strongly recommend using the safety gloves included with the standard delivery!

• • • • •

Never leave knives and knife/blade holders with a knife/blade mounted lying around! Never place a knife on a table with the cutting edge facing upward! Never try to catch a falling knife! Always clamp the specimen first, then the knife. Before handling the specimen or the knife, changing the specimen or taking a break, lock the handwheel and ensure that the knife is covered by the knife guard. • Extended skin contact with the cold parts of the instrument may cause frostbite! • To make sure that the condensation water stemming from the defrost cycles drains into the waste container and to avoid the risk of possible contamination, ensure that the tap of the waste container (2, Fig. 54) is open whenever operating the cryostat. Only shut the tap when draining the waste container!

2.6

Cleaning and disinfection We strongly recommend defrosting the CM1860/CM1860UV Cryostat completely for 24 hours, 1-2x annually or as needed. • It is not necessary to remove the microtome for routine disinfection. The instrument has been designed for UV disinfection. Spray disinfection with Leica Cryofect* is also possible, thanks to the special insulation of the microtome. Remove section waste after EVERY change of specimen. Do not start disinfection until you have done this! Each new specimen is a potential source of contamination. The use of flammable sprays within the cryostat chamber is permitted only with the Leica Cryofect tested by us.

At cryochamber temperatures of 0 °C to -35 °C! Spray a MAXIMUM of 10 sprays into the middle of the cryostat chamber and close the sliding glass door. After each disinfection, the chamber must be wiped carefully with a cloth and briefly ventilated! * Cryofect is not sold in all parts of the world

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Instructions for Use V 1.3 – 09/2012

2.   Safety • Do not use organic solvents or other aggressive substances for cleaning. Use only disinfectants listed in these Instructions for Use, such as Leica Cryofect (alcohol or other common alcohol-based disinfectants). Please contact Leica Biosystems for further details on adequate disinfection measures.

2.7

Removing the microtome • The microtome is encapsulated and therefore does not require removal by the user.

2.8

Maintenance Replacing the fuses • Turn the instrument off and disconnect the power plug before replacing the fuses! • Use only the fuse types specified in Chapter 3, "Technical Data". Failure to comply with this can cause severe damage to the instrument and its surroundings! Replacement of the lamp/UVC lamp If both disinfection indicator lights are flashing alternately, the UV tube must be replaced!

• Turn the instrument off and disconnect the power plug before replacing the lamps. (For more details, refer to Chapter 9, "Cleaning, Disinfection, Maintenance.) It is possible to break the UVC lamp during replacement. If this happens, the lamp change must be completed by Technical Service. If any metallic mercury is released, handle it carefully and dispose of it properly.

Leica CM1860 /CM1860UV – Cryostat

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3.

Technical Data

Type

CM1860 / CM1860 / CM1860 / CM1860 / CM1860 UV CM1860 UV CM1860 UV CM1860 UV Test symbol c_CSA_us Nominal voltage 100 VAC ±10 % 120-127 VAC ±10 % 230 VAC ±10 % 220-230 VAC ±10 % Nominal frequency 50/60 Hz 60 Hz 60 Hz 50 Hz Power input 1440 VA 1440 VA 1440 VA 1440 VA Max. start-up current for 5 sec 45 A eff. 45 A eff. 45 A eff. 45 A eff. Protective class I I I I Pollution degree  2 2 2 2 Overvoltage inst. category II II II II Heat emission (max.) 1600 J/s 1600 J/s 1600 J/s 1600 J/s

CM1860/ CM1860 UV 240 VAC ±10 % 50 Hz 1440 VA 45 A eff. I 2 II 1600 J/s

 according to IEC-1010, UL 3101 Operating temperature range (ambient temperature): 18 °C to 35 °C. All specifications related to temperature of the cooling unit are valid only for an ambient temperature of 22 °C and a relative humidity of no more than 60 %! Refrigeration system Cryochamber Temperature range Cooling time down to -35 °C Defrost

50 Hz/60 Hz

Refrigeration capacity Safety factor Refrigerant Compressor oil

0 °C to -35 °C (+ 3 K/- 3 K) max. 6 hrs., at 22 °C ambient temperature Automatic hot gas defrost 1 automatic defrost cycle/24 hours, time-controlled (duration 12 min.) 690 W 3 300g ±5g refrigerant R 404A * 0.6 l EMKARATE RL-22S, ICI *

Quick-freeze shelf Maximum temperature Number of freezing stations Defrost

- 40 °C (+ 3 K/- 5 K) 8 Manual hot gas defrost, time-controlled (duration 12 min.)

Peltier element Max. temperature difference Number of freezing stations Defrost

17K, at - 35 °C chamber temperature 2 In conjunction with the quick-freeze shelf

 according to CECOMAF Liquid temperature 45 °C, evaporation temperature -25 °C 16

Instructions for Use V 1.3 – 09/2012

3.   Technical Data *) Refrigerant and compressor oil must be replaced by qualified, authorized service personnel only.

Microtome Rotary microtome Section thickness selection: Total specimen feed Vertical stroke Maximum specimen size Specimen orientation Electric coarse feed

Lamp 1 - 100 µm 25 mm 59 mm 55 x 55 mm or 50 x 80 mm 8° (x, y, z-axis)

Slow Rapid

50 Hz version: 60 Hz version:

Osram Dulux S 11 W/21 Color: LUMILUX light white Osram Dulux S 13 W/21 Color: LUMILUX light white

600 µm/s 900 µm/s

Cryocabinet Dimensions Width (w/o handwheel) Width (with handwheel) Depth Height Weight (incl. microtome, without specimen cooling)

600 mm 730 mm 730 mm 1140 mm

Storage conditions

Temperature: Humidity: Transport temperature:

5 - 50 °C <80 %, non-condensing -40 °C to +55 °C

Observe item 4.1, "Installation site ­requirements". approx. 135 kg

All CM1860/CM1860 UV instrument variants need the following secondary fuses: F1: T0.25A L250V Type FST 6.3x32 Brand: SCHURTER F2: T1.6A L250V Type FST 6.3x32 Brand: SCHURTER F3: T1.0A L250V Type FST 6.3x32 Brand: SCHURTER F4: T6.25A L250V Type FST 6.3x32 Brand: SCHURTER F5: T4A L250V Type FST 6.3x32 Brand: SCHURTER

Leica CM1860/CM1860 UV – Cryostat

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4.

Setting up the Instrument

4.1

Installation site requirements Do not operate in rooms with explosion hazard! To ensure proper function of the instrument, it must be set up with a minimum distance on all sides from walls and furniture (see "Transport and installation").

The place of installation must meet the following requirements: - The instrument requires an installation area of approx. 600 x 730 mm. - Room volume must be at least 8 m3. - Room temperature consistently 18 °C - 35 °C. - Temperature range during storage: 5 °C - 50 °C - Relative humidity, maximum 60 % (non-condensing), - Elevation: up to a max. of 2000 m above sea level Condensation water may form in the instrument if there is an extreme difference in temperature between the storage location and the installation site and if air humidity is high at the same time. If this is the case, wait at least two hours before switching on the instrument. Failure to comply with this may cause damage to the instrument.

• The instrument is designed for indoor use only. • The power plug/circuit breaker must be freely and easily accessible. The power socket may not be located further than 2.5 m from the instrument. • Power supply at a distance no greater than the length of the power cable – an extension cable must NOT be used. • The floor must be largely vibration-free and have sufficient load capacity and rigidity for the weight of the instrument. • Avoid impacts, direct sunlight, and excessive temperature fluctuations. • The instrument MUST be connected to a properly grounded socket. Use only the power cable provided, which is intended for the local power supply. • The chemicals to be used are easily inflammable and hazardous to health. Therefore the installation location must be well ventilated, and must contain no sources of ignition of any kind. • Furthermore, this instrument must NOT be operated directly under the outlet of an airconditioning system, since the increased air circulation accelerates icing of the chamber. • The installation location must be protected against electrostatic charge. Noise information: A-weighted noise level: <= 70 dB (A) Room temperatures and humidity levels in excess of the recommendations above will affect the cryostat's cooling capacity and the lowest stated temperatures will not be reached.

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Instructions for Use V 1.3 – 09/2012

4.

Setting up the Instrument

4.2

Unpacking instructions for the cryostat CM1860/1860 UV When the instrument is delivered, check the tilt indicators on the packaging. If the arrowhead is blue, the shipment was transported laying flat, was tilted at too great an angle or fell over during transport. Note this on the shipping documents and check the shipment for possible damage.

Fig. 4 Caution when removing the metal strips! There is a risk of injury when opening them (the strip has sharp edges and is under tension)!

2



 1

To remove the metal strips (1), you need metal shears and suitable gloves. Stand next to the crate and cut the strips at the location shown (see Fig. 2a "").

Fig. 4a

3

• Lift the outer carton ring (2) upwards and out. • Remove the foam transport anchors (3), and then pull the dust cover from the instrument. • Remove all accessories (4).

3

4

5

Fig. 4b

Leica CM1860 /CM1860UV – Cryostat

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4.

Setting up the Instrument 8

Transport grip points





Fig. 4c

Fig. 4d

6

• Lift and remove the wooden edge (6). • Remove the ramp (5) on the front side of the pallet (see Fig. 4b) and correctly attach it to the back (see Fig. 4d).

5

7

5

Do not push the device by its hood (8)! Use the transport grip points (9 )! The rollers (7) must run over the ramp (5) at the front and rear. Danger of tipping!

• Carefully roll the instrument backwards over the ramp from the pallet. • Push the instrument to the installation location on the castors (7). The instrument must be transported in an upright position.

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Instructions for Use V 1.3 – 09/2012

4.   Setting up the Instrument Transport to the site • First, check if the location meets the conditions specified in 'Installation site requirements'. • Transport the instrument to the desired location. • Observe the following: The instrument must be transported in an upright position or slightly tilted (max. 30°)! When tilting the instrument 2 people must counterbalance from the front side to prevent the instrument from falling down and causing severe injury, as well as possible severe damage to the instrument and the surrounding area!

• When transporting the instrument on wheels, (2) grip the cabinet only at the marked locations ( ). • To ensure a secure upright position at its intended location, the instrument must be adjusted. To do so, unscrew both adjusting feet (1) using the provided open-end wrench (No. 13). When subsequently transporting the instrument any further on wheels, screw them back in all the way. 2 (not visible)

1

2 1

Leica CM1860 /CM1860UV – Cryostat

2

Fig. 9

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