M-Series User manual V11.2001

Contents

Page Overview Safety concept... 4 Symbols... 5 Controls and functions... 6 Short instructions... 8 Use Ergonomics... 9 Viewing height and interpupillary distance... 9 Eyepoint, eye contact... 9 Changing the magnification... 10 Focusing... 11 Stereoscopic and axial observation... 12 Dioptric settings... 12 Working with the stereomicroscope... 14 Assembly Basic outfit Overview: Components... 15 Spacer rings... 16 Fitting the column to the incident- and transmitted-light bases... 18 Microscope carrier... 18 Binocular tubes, optics carrier, optical accessories... 19 Graticules... 20 Stands Transmitted-light stand, bright field, 20W... 20 Transmitted-light stands, bright and dark field... 22 Swinging-arm stand ESD... 22 Large swinging-arm and table-clamp stands... 24 Illuminators Transformers... 26 Leica L2 cold-light source...27 Fibre-optic light guide... 28 Cold-light source Leica CLS Series...28 Mains (line) lamp 25W... 28 Incident lamp 6V/10W... 28 Incident lamp 6V/20W... 30 Coaxial illuminator... 32 Near-vertical illuminator... 32

2

Page Accessories Fitting accessory tubes... 33 Double-iris diaphragm... 33 Drawing tube... 33 Video-phototubes: Photography, video, TV, filming, digital imaging... 34 Attachment for vertical and oblique observation... 37 Stages, polarization... 37 Special notes Tips and hints: What to do if …... 38 Care and maintenance of the stereomicroscope, maintenance... 39 Calculating total magnification and field diameter... 40 Optical data, MS5, MZ6... 41 Optical data, MZ75, MZ95... 42 Optical data, MZ125, MZAPO... 43 Dimensions... 44

3

To you, the user

Prohibited uses •

The use of the instrument in a different manner from that described in this user manual can lead to injury, malfunction and damage.

•

Do not fit different plugs. Do not dismantle optical systems or mechanical parts unless instructions for doing so are given in the user manual.

Thank you for your trust. We wish you success and satisfaction in your work with your new stereomicroscope from Leica. The innovative technology of the new stereomicroscope line from Leica is expressed externally in the avant-garde form of the instrument. The rounded shapes, with their elliptical tendencies, demonstrate that beauty can be practical.

Place of use In developing this new stereomicroscope line we have placed great weight on simple, self-explanatory directions. We have however put a lot of effort into explaining all functions to you by means of pictures and text. You will only get the best out of your stereomicroscope if you take the trouble to study this user manual in detail. If you still have a question sometime, just contact your local Leica agency or get in touch directly with Leica Microscopy Systems Ltd, Heerbrugg, Switzerland. We are gladly at your service. Customer service is a big thing with us. Not only before the sale, but afterwards as well.* * The business units of Leica Microsystems hold the management system certificates for the international standards ISO 9001 and ISO 14001 relating to quality management, quality assurance and environmental management.

•

The Leica MS5, MZ6, MZ75, MZ95, MZ125 and MZAPO stereomicroscopes are intended mainly for indoor use.

•

If the stereomicroscope is used outdoors, protect it from dirt and damp. Lamps and electrically-operated stands from Leica may not be used outdoors.

Use in ESD protected rooms The optics carrier MS5, MZ6, MZ75, MZ95, MZ125, the binocular tube, ErgoTubus™, ErgoKeil™ 5°–25°, the swinging-arm stand ESD and the cold light sources Leica L2 and CLS consist of ESDdischarging material (surface resistance (<1011 ohms/squared, discharge time <2 seconds, 1,000V to 100V). •

A connection socket for a 4mm dia. earthing cable is provided on the microscope carrier.

•

There are two connection sockets for 4mm dia. earthing cables in the base of the swinging-arm stand.

Servicing Repairs may only be carried out by Leica-trained service technicians. Only original Leica spare parts may be used.

Safety concept General directions Before you try to use the instrument, please read the user manual and the safety notes. Permitted uses The Leica MS5, MZ6, MZ75, MZ95, MZ125 and MZAPO stereomicroscopes are optical instruments which use magnification to see the finer details of objects close to the eye. The objects may be either natural, or created by human agency. The basic outfit, which includes a stand and a light source, can be supplemented with various modules for photography, TV, dual station viewing and other applications.

4

Responsibilities of person in charge of instrument •

Ensure that personnel who use the instrument have read and understood this user manual and in particular the safety instructions.

•

Ensure that the Leica MS5, MZ6, MZ75, MZ95, MZ125 and MZAPO stereomicroscopes are operated, maintained and repaired only by authorized and trained personnel.

Safety regulations Electrically-operated items

Changing bulbs •

Before changing a bulb, disconnect the power cable.

•

Never attempt to touch the inside of a lamp housing or transmitted-light stand while the instrument is connected to the power supply.

•

Before changing a bulb, wait until it is cool, otherwise you may burn your fingers.

The safety regulations listed below apply to the following items: – Transmitted-light stand, bright field, 20W (page 20) – Mains lamp 25W (page 28) – Regulating transformer 0–7V/40W (page 26) – Step transformer 4/5/6V (page 26) – Motor focus (separate instructions)

Legal requirements Adhere to general and local regulations relating to accident prevention and environmental protection.

Liquids Be careful when handling liquids. If spilt on the equipment, they can: – cause the stereomicroscopes and accessories to become electrically live, endangering personnel – cause damage to the equipment. Power cables Inspect cables regularly for damage. Defective cables can:

Conformity with European Community directive The electrically-operated accessories for the Leica MS5, MZ6, MZ75, MZ95, MZ125 and MZAPO stereomicroscopes are constructed in accordance with the latest technologies and are provided with a statement of conformity with EC requirements.

You will find these symbols in the user manual: Safety direction

– directly endanger personnel

This symbol denotes information which must be read and obeyed. Failure to respect it can cause injury Failure to respect it can cause malfunctioning, or damage to the equipment

– cause the stereomicroscopes and accessories to become electrically live, endangering personnel. Positioning cables Position cables with care. Make sure that personnel do not get entangled with them. The instrument could fall over, fall down, become damaged, damage other equipment, or cause personal injury.

Warning about accessible hot places, e.g. bulbs

Opening the instrument

Important information

Only authorized Leica personnel are permitted to repair electrical equipment. Before opening up the equipment, pull out the power cable. If you touch the live interior of the equipment, you may receive an electric shock.

Failure to respect it can cause burns

This symbol denotes additional information or explanations which promote understanding. Action 왘 This symbol within the text indicates that certain operations must be carried out.

Grid voltage Ensure that the voltage setting on the equipment is correct. A wrong setting can cause damage to the equipment. Connections

Explanatory notes •

This symbol within the text stands for additional information and explanations.

When connecting power-consuming devices to the transformers, do not exceed the maximum permitted loading, as this can cause damage to the equipment.

5

Overview

6 Interchangeable objective Various objectives (achromatic, planachromatic, planapochromatic), depending on the outfit.

Standard outfit: Controls and functions In this overview we explain the controls and functions relating to the standard outfits.

Binocular tube 7 Adjustable eyepiece tubes: Interpupillary distance adjustable within the range 52mm – 76mm.

Does your stereomicroscope look different from the one in the picture?

8 Wide-field eyepieces for spectacle wearers

The modular design enables you to assemble an outfit matched to the application. Controls and functions which differ from those in the standard outfit are described in the appropriate section (see contents list on page 2). Leica MZAPO see page 8.

9 Clamping screws

Stereomicroscope

Dioptric setting adjustable within the range from +5 to –5, and eyecups adjustable.

These hold the eyepieces in the binocular tube. 10 ErgoTube™ The viewing angle is adjustable within the range 10° – 50°.

1 Magnification changer Drive knobs with magnification scale. MS5: 5 steps MZ6: 6:1 zoom

Leica Design by Ernest Igl / Christophe Apothéloz

MZ75: 7.9:1 zoom MZ95: 9.5:1 zoom MZ125: 12.5:1 zoom MZAPO: 10:1 zoom.

MS5

2 Engaging ratchet positions MZ6: Switch engages 7 magnification steps MZ75: Milled ring engages 8 magnification steps

8

MZ95: Switch engages 9 magnification steps MZ125: Switch engages 10 magnification steps MZAPO: Switch engages 9 magnification steps. 3a Focusing drive 3b Coarse and fine focusing

9 7 5

Larger knob: Coarse focusing Smaller knob: Fine focusing. 3c Adjustable ring Changes the ease of movement of the coarse/fine drive. 4 Clamping screw Holds the optics carrier in the microscope carrier. 5 Clamping screw Holds the binocular tube (or accessory) to the optics carrier. 6

1 4 6 3a

MZ75

MZ6

8 8 7

9 7

5

5

2

1 1

4 6

2

4 6

3c 3b

3a

MZ95

MZ125

8

8

7

7

5

10 5 1 2

4 6

3c 3b

1 2 4 6

3c 3b

7

Short instructions

Inspect the cleanliness

Checklist

왘 Clean: Optical accessories, glass stage plates, and any graticules used (page 39).

왘 Remove dust and dirt from the instrument (page 39).

In this overview Only for variable ErgoModules™

we have summarized the working sequence. You will find detailed user instructions on the pages indicated.

왘 Adjust viewing height (page 9) Only for photomicrographic outfits 왘 Lever at position “VIS”. 왘 For trinocular tube: Push diaphragm lever to the right. 왘 Open double-iris diaphragm (position “OPEN”). Adjust the interpupillary distance 왘 Using both hands, displace the eyepiece tubes (page 9). Select the degree of eye contact 왘 Secure the eyecup in the position required (page 9). Adjust the dioptric setting 왘 Adjust the dioptric setting precisely for each eyepiece (page 12).

MZAPO Set the magnification required

8

왘 Turn the rotary knob to the desired magnification (page 10). Focusing

9 7

왘 Look into the eyepieces and focus on the object (page 11). •

10

Only for coarse/fine drive: Focus approximately with the large knob, and then accurately with the small knob.

5 2

왘 If the focusing movement is too loose or too tight, adjust the ease of movement (page 11).

1 4 6

3b

Adjust the depth of field

3c

왘 Adjust the depth of field with the double-iris diaphragm (page 33). Microscope carrier AX 왘 For measuring, photography and polarization, engage the vertical beam path (page 12).

8

Use

Interpupillary distance •

Ergonomics, viewing height, interpupillary distance, eye contact

The interpupillary distance is correct when you see a single circular field when viewing with both eyes. 왘 Look into the eyepieces and use both hands to move the eyepiece tubes closer together or further apart.

Ergonomics The reasons why users often cannot work comfortably with the 45° tube are the differing heights of the outfits, the various accessories and working distances, and the fact that the same instrument is often used by several people of differing builds. This problem can be effectively solved by using the Leica ErgoModules™. If you have problems with the viewing configuration on your stereomicroscope, ask your Leica consultant for the most ergonomic Leica solutions. Viewing height, viewing angle •

The viewing height is correct when your head and back are comfortably positioned. •

Locating the eyepoint •

For 10×/21B wide-field eyepieces for spectacle wearers, the distance from eye to eyepiece is about 22mm.

•

When you can see the entire field of view without restriction, you have found the correct position (eyepoint). 왘 To find the eyepoint, slowly bring your eyes closer towards the eyepieces.

On the ErgoTube™, the viewing angle is adjustable from 10° – 50°.

왘 Holding the eyepiece tubes at their bases, swing them up or down (see arrow).

Selecting the degree of eye contact If you do not wear spectacles, and want closer contact to the eyepieces: 왘 Holding the diopter ring, turn the eyecup anticlockwise until it is released.

ErgoModule™ •

ErgoWedge™ 5° – 25°

•

ErgoWedge™ ±15°

•

ErgoModule™ 50mm

•

The ErgoTube™ 45° has eyepiece tubes which are 65mm longer than the standard ones.

•

ErgoModule™ 30mm – 120mm •

왘 Pull the eyecup upwards. 왘 Holding the diopter ring, turn the eyecup clockwise.

After several years, regreasing may be necessary. Send the ErgoModule™ to the Leica service workshop. Spectacle wearers need the whole of the available distance. Move the eyecups to the lowest position.

9

Use

Scale of magnifications

Magnification changer

MS5, MZ6

MS5: 5 steps MZ6: 6:1 zoom MZ75: 7.9:1 zoom MZ95: 9.5:1 zoom MZ125: 12.5:1 zoom MZAPO: 10:1 zoom

Display of magnification factors from 0.63 – 4.

•

MZ75 Display of magnification factors from 0.63 – 5. For other objectiveeyepiece combinations, appropriate adhesive scales can be applied to the rotary knobs (page 19).

Zoom means continuous change of magnification. Ratchets can be engaged for certain magnifications (see below).

MZ95 Display of magnification factors from 0.63 – 6.

Changing the magnification •

The magnification changer can be operated from either the right or the left.

MZ125 Display of magnification factors from 0.8 – 10.

왘 Look into the eyepieces. 왘 Focus on the object (page 11).

MZAPO

왘 Turn the magnification changer to the position required.

Display of magnifications with various optical combinations, e.g. with 1.0× objective and 10× eyepieces: 8× – 80×. MZAPO: Setting the scale The magnification scale can be set in accordance with the optical combination.

Ratchet steps MZ6: 0.8, 1.0, 1.25, 1.6, 2, 2.5, 3.2 MZ75: 0.8, 1, 1.25, 1.6, 2, 2.5, 3.2, 4 MZ95: 0.8, 1, 1.25, 1.6, 2, 2.5, 3.2, 4, 5 MZ125: 1, 1.25, 1.6, 2, 2.5, 3.2, 4, 5, 6.3, 8 MZAPO: 10, 12.5, 16, 20, 25, 32, 40, 50, 63 •

Engage the ratchets whenever magnifications need to be exactly reproducible. 왘 For MZ6, MZ95, MZ125 and MZAPO: Press the switch.

Example: 1.0× objective / 25× eyepiece 왘 Turn the magnification changer to the stop at its lowest magnification position. 왘 Read the magnification range from the table on page 43 (in the example given, 20× to 200×). 왘 Place a finger in the rear window of the magnification changer and displace the scale so that the position 20 is lined up with the index. Magnifications and fields of view

왘 For MZ75: Turn the knurled ring anticlockwise.

10

The tables between page 41 and page 43 provide information about the magnifications and field diameters as a function of the magnification changer position of the eyepiece-objective combination used.

Magnification with MZ125/MZAPO Pan and Planapo objectives

Adjusting the free running of the focusing drive

When the MZ125/MZAPO planachromatic and planapochromatic objectives are used on the Leica MS5, MZ6, MZ75 and MZ95, the magnification is increased by the factor 1.25×. This factor is taken into account in the tables.

Does the focusing drive run too loosely or too tightly, or does the equipment drift downwards? You can match the ease of movement to the weight of the outfit and to your personal requirements.

Magnification on MZ125/MZAPO

Magnification on MS5/MZ6/MZ75/MZ95

1.0× Plan/Planapo

1.25×

1.6× Planapo

2.0×

0.63× Planapo

0.8×

0.5× Plan

0.63

0.8× Plan

1.0×

Setting the working distance, focusing = obtaining a sharp picture •

To focus, use the focusing drive to raise or lower the stereomicroscope until the feature of interest is in focus. The working distances of the various objectives are listed in the tables on pages 41–43.

•

The focusing drive can be operated from either the right or the left.

Focusing drive 왘 Grip the drive knobs firmly with both hands and turn them in opposite directions until you obtain the required degree of resistance during focusing

Focusing drive, coarse / fine 왘 Turn the black ring on the right-hand drive knob until you obtain the required degree of resistance during focusing.

왘 Place the object beneath the objective.

왘 Engage the lowest magnification (page 10). 왘 Look into the eyepieces. 왘 Using the rotary knob, bring the object into focus.

Only for coarse / fine drives 왘 Focus approximately with the larger knob. 왘 Focus accurately with the smaller knob.

11

Use

Microscope carrier AX

Microscope carrier: Turn optics carrier sideways, stereoscopic / axial observation Microscope carrier for stereoscopic observation This microscope carrier permits only stereoscopic observation, using both beam paths. •

The optics carrier can be turned sideways in the microscope carrier if the user needs to work from the side: 왘 Release the clamping screw. 왘 Turn the optics carrier sideways as required. 왘 Retighten the clamping screw.

The stereomicroscopes can be equipped with the microscope carrier AX for stereoscopic and axial observation instead of the standard microscope carrier. •

The microscope carrier cannot be turned sideways when in the microscope carrier AX (see page 24).

•

The microscope carrier AX can only be fitted in one position to the focusing drive (see page 18).

For the Leica MZ125 and Leica MZAPO, use the spacer ring (page 16). Stereoscopic observation A three-dimensional image is essential for studying and working on spatial objects. 왘 Shift the stereomicroscope to the central position. Axial observation

The microscope carrier can be attached to the focusing drive in either of two positions: •

1

•

2

For assembly, refer to page 18.

12

For short working distances and for flat objects, use Position 1. Use this position also in conjunction with the attachment for vertical and oblique observation (see detailed instructions).

Use Position 2 for long working distances and for bulky objects.

The parallax-free imaging produces more precise results in photography, measuring and polarization. 왘 Shift the stereomicroscope to the left or the right position. The object is now located directly beneath either the left or the right beam path. •

When photographing or measuring, select the beam path above which the phototube or measuring graticule is located.

Adjusting the dioptric settings and the parfocality You must adjust the dioptric settings for both eyes exactly as described on page 13. Only then will the image be parfocal, i.e. it will remain sharp whenever the magnification is changed and there will be no need to refocus. Make the most of this opportunity, which is not offered by every stereomicroscope. •

The dioptric range is from +5 to –5.

The following adjustments must be carried out only once by each user. The procedures for adjusting the dioptric setting and the parfocality are somewhat different if an eyepiece contains a graticule. Refer to the user manuals for the MPS modular photomicrographic systems or for measuring.

C Adjust the dioptric settings 6. Lowest magnification 왘 Engage the lowest magnification position.

A Preparations 왘 Set up the illumination (pages 20–21, 26–32). 7. Do not look into the eyepieces

왘 Set the interpupillary distance and, if necessary, the viewing height (page 9).

왘 Rotate the eyepieces anticlockwise in the “+” direction as far as the stop (+5 diopters).

왘 On the video-/phototube: Move the lever to position “VIS” and open the diaphragm. 왘 Set the double-iris diaphragm to “OPEN” (page 33). 왘 Adjust the eyecups (page 9). 왘 Set both eyepieces to zero.

8. Look into the eyepieces

왘 Set the microscope carrier AX for stereoscopic observation.

왘 Slowly rotate each eyepiece individually in the “–” direction until each eye sees the object sharply imaged.

B Focus on the test object 1. Flat test object 왘 Place such an object beneath the objective. D Inspect the parfocality

9. Highest magnification 왘 Engage the highest magnification position. 2. Lowest magnification 왘 Engage the lowest magnification position (page 10). 왘 Use the coarse focusing drive to set the working distance (page 11). 3. Look into the eyepieces 왘 Using the focusing drive, bring the test object into focus. 4. Highest magnification 왘 Engage the highest magnification position.

10. Refocus 왘 Refocus if necessary.

11. Magnification changer 왘 Slowly move the magnification changer from the lowest magnification to the highest. •

The image sharpness must remain constant (parfocal). Otherwise, repeat the procedure.

5. Image sharpness 왘 Optimize with focusing drive.

13

•

Use Working with the stereomicroscope In this section we will show you how to work smoothly with your stereomicroscope and how to study objects routinely.

When focusing on a fresh object, always start with the lowest magnification, because the larger field of view makes the object easier to find. Afterwards, select the magnification you want. 왘 Place the object beneath the objective. 왘 Illuminate the object.

Preconditions for working comfortably •

Pay great attention when setting up your stereomicroscope. You will only benefit fully from the outstanding optical and ergonomic advantages of the instrument if you set it up precisely in accordance with the directions already given.

•

For fatigue-free work, use the ErgoModules™ (page 9).

•

Arrange your workplace as well as possible, and pay attention to the heights of chair and table.

•

When sitting, use the entire seat area and also the back support of the chair.

•

Support your forearms.

•

During work breaks, stand up and move around for exercise. Tips for your work The stereomicroscopes are parfocally matched. This means that, once you have focused on a feature in the manner described, taking account of dioptric settings (page 12), you can zoom the magnification up and down without refocusing. You only need to refocus if you want to look at higher or lower planes in the object.

14

왘 Select the lowest magnification position. 왘 Using the focusing drive, set the working distance approximately (page 11). 왘 Look into the eyepieces and use the focusing drive to bring the object precisely into focus. 왘 Select the highest magnification position. 왘 Use the focusing drive to refocus. Now the object should remain in focus at all zoom magnifications. Otherwise, check that your dioptric settings have been adjusted exactly as described on page 12.

Assembly Basic outfit The components 1a Incident-light base with stage plate 1b Transmitted-light base with glass stage plate 2a Side-faced column with drive housing, coarse/fine 2b Side-faced column with drive housing 3 Microscope carrier 4 Optics carrier 5 Interchangeable objective 6 Binocular tube 7 Wide-field eyepiece for spectacle wearers, with eyecups 8 Various ErgoModules™ or accessories for video, photography, drawing, fluorescence, coaxial incident light etc.

7

8 6

4 5 2b

3

2a 1a

1b

15

Spacer rings Spacer rings are required in order to attach the objectives for certain instrument configurations. •

The Leica MZ95 is supplied with a spacer ring for the smaller-diameter achromatic objectives and 1.0× planachromatic objective. When the spacer ring has been removed by applying the tool provided, the larger-diameter planachromatic and planapochromatic objectives for the MZ125/MZAPO can be used.

•

The Leica MS5, MZ6 and MZ75 can also be equipped with the larger-diameter planachromatic and planapochromatic objectives for the MZ125/MZAPO if a spacer ring is fitted.

•

When the MZ125/MZAPO planachromatic and planapochromatic objectives are used on the Leica MS5, MZ6, MZ75 and MZ95 stereomicroscopes, the magnification is increased by a factor of 1.25×.

10 445 821 MZAPO 10 446 370 MZ12 5

10 446 272 MZ9 5 10 446 371 MZ7 5 10 445 614 MZ6 10 445 613 MS5

10 446 172

10 411 589 1x 10 422 562 1.5x 10 422 561 2x 10 473 832 0.8x 10 445 201 0.63x 10 422 563 0.5x 10 422 564 0.32x Achromat

10 446 275 1x Plan 10 446 157 0.5x 10 446 230 1x 10 472 650 1.6x 10 446 236 0.63x 10 447 075 0.8×

10 411 589 1x 10 422 562 1.5x 10 422 561 2x 10 473 832 0.8x 10 445 201 0.63x 10 422 563 0.5x 10 422 564 0.32x Achromat

10 446 275 1x Plan

10 445 819 1x Plan 10 446 157 0.5x 10 446 230 1x 10 472 650 1.6x 10 446 236 0.63x Plan/Planapo

10 367 929 10 315 306 10 445 352 10 367 929 10 315 306 10 445 352

16

Objective combinations

Article no.

MS5

MZ6

MZ75

MZ95

Achromat 1×

10 411 589

C

C

C

C

Achromat 1.5×

10 422 562

C

C

C

C

Achromat 2×

10 422 561

C

C

C

C

Achromat 0.8×

10 473 832

C

C

C

C

Achromat 0.63×

10 445 201

C

C

C

C

Achromat 0.5×

10 422 563

C

C

C

C

Achromat 0.32×

10 422 564

C

C

C

C

MZ125

MZAPO

C

C

Achromat

Plan Plan 1×

10 446 275

C

C

C

C

Plan 1× MZ125/MZAPO

10 445 819

CA (10 446 172) M

CA (10 446 172) M

CA (10 446 172) M

C*M

Plan 0.5× MZ125/MZAPO

10 446 157

CA (10 446 172) M

CA (10 446 172) M

CA (10 446 172) M

C*M

C

C

Plan 0.8×

10 447 075

CA (10 446 172) M

CA (10 446 172) M

CA (10 446 172) M

C*M

C

C

Planapo 1× MZ125/MZAPO

10 446 230

CA (10 446 172) M

CA (10 446 172) M

CA (10 446 172) M

C*M

C

C

Planapo 1.6× MZ125/MZAPO

10 472 650

CA (10 446 172) M

CA (10 446 172) M

CA (10 446 172) M

C*M

C

C

Planapo 0.63× MZ125/MZAPO

10 446 236

CA (10 446 172) M

CA (10 446 172) M

CA (10 446 172) M

C*M

C

C

Coaxial incident light

10 446 180

C

C

O (10 446 300)

O (10 446 300)

C

C

Microscope carrier AX

10 445 618

C

C

C

C

CA (10 446 172)

CA (10 446 172)

Near-vertical illuminator

10 445 198

C

C

CA 2× (10 446 300)

CA (10 446 300)

CA (10 446 300)

CA (10 446 300)

Planapo

Accessories

Attachment for vertical

10 445 156

C

and oblique observation C

C

CA 2× (10 446 300)

CA (10 446 300)

+ (10 446 393)

+ (10 446 393)

CA (10 446 300)

CA (10 446 300)

+ (10 446 393)

+ (10 446 393)

Compatible

CA Compatible if used with intermediate ring (order separately) 0

Intermediate ring recommended

M Magnification increased by factor 1.25× *

Remove intermediate ring (10 446 393); already included with MZ95.

17

Assembly

Microscope carrier, optics carrier, binocular tube, optical accessories

In the following sections we will explain how to assemble a complete stereomicroscope outfit, including the incident- and transmitted-light stands. Detailed directions about the transmitted-light stands, the swinging-arm stand and the fitting of the remaining accessories are all described in the appropriate sections (see contents list on page 3). Side-faced column ➜ incident-light base 왘 Remove the stage plate. 왘 Introduce 3 Allen screws into the underside of the baseplate and secure them to the bottom end of the side-faced column.

In this section we will explain how to fit the microscope carrier and the optics carrier to the incident- and transmitted-light stands. The combination of the optics carrier with the inclinable drive housing is described on page 22. The Leica motor focus system is described in a separate user manual. Side-faced column ➜ drive housing The microscope carrier can be fitted in either of two positions to the drive housing (refer also to page 12): 왘 Use an Allen screw to secure the microscope carrier in the position required.

왘 Insert the stage plate.

The side-faced column with focusing drive is available with column lengths 300mm and 500mm. To fit the microscope carrier and the stereomicroscope, see the next column of text. Side-faced column ➜ transmitted-light base Transmitted-light stand, bright field, 20W: Pull the power cable out of the socket in the stand. •

Transmitted-light stand for bright and dark field: Pull the fibre-optic light guide out of the connector in the stand. 왘 Remove the glass stage plate. 왘 Carefully invert the transmittedlight base and remove 7 Allen screws from the base.

A connection socket for a 4mm dia. earthing cable is provided on the microscope carrier.

왘 Lift off the lower part of the base. 왘 Reverse the upper part of the base. 왘 Introduce 3 Allen screws into the cover plate from beneath and secure them to the bottom end of the side-faced column.

Microscope carrier AX •

The microscope carrier AX can be fitted to the drive housing only in position 1 (see above).

왘 Insert the glass stage plate.

•

왘 Fit the microscope carrier and stereomicroscope as follows:

The optics carrier cannot be turned sideways in the microscope carrier AX.

•

The Leica MZ125 and Leica MZAPO require a spacer ring (page 16) in order to secure the planachromatic and planapochromatic objectives to the microscope carrier AX.

왘 Replace and secure the base.

18

Optics carrier ➜ microscope carrier

Binocular tube 왘 Release the clamping screw.

왘 Gently lower the optics carrier into the microscope carrier.

•

왘 Use the screw to secure the optics carrier in the position required.

Apply the adhesive scale of magnifications

If you have an accessory such as an ErgoModule™ (page 9), a coaxial illuminator (page 32), a video-/phototube (page 35) or a filter-slide holder (page 35), fit it now to the optics carrier. Tighten the clamping screw.

왘 Tilt the binocular tube and insert the edge of its dovetail ring beneath the two lugs on the optics carrier.

Only for MZ75: 왘 Remove the screws. 왘 Remove the rotary knobs.

왘 Move the binocular tube from side to side until the locating screw on the optics carrier engages the guide.

왘 Apply the adhesive scales. 왘 Replace the rotary knobs.

왘 Tighten the clamping screw. Objective 왘 Screw on the selected objective anticlockwise.

Eyepieces 왘 Insert a graticule if appropriate (page 20). 왘 Release the clamping screws on the eyepieces. 왘 Insert the eyepieces fully into the eyepiece tubes. Check that they have seated correctly.

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Some instrument configurations require spacer rings in order to secure the objectives (page 16). These rings are fitted and removed using the tool provided.

왘 Tighten the clamping screw.

The Leica MZ95 is supplied with a spacer ring for the achromatic objectives and for the 1.0× planachromatic objective. When the ring has been removed by means of the tool provided, the larger-diameter planachromatic and planapochromatic objectives for the MZ125/MZAPO can be fitted. •

You can extend the overall magnification range by using 16×, 25× and 40× wide-eyepieces for spectacle wearers.

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Graticules •

Stands

The graticules for measuring and counting are in mounts which can be fitted directly into the eyepieces.

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A highly-precise stage micrometer with a 50mm scale and 0.1mm and 0.01mm divisions enables the graticule to be calibrated in accordance with the magnification selected.

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It is best to measure with the microscope carrier AX in vertical position. The measurements are parallax-free and therefore more precise.

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Focusing and framing graticules indicate the format limits for all films.

Transmitted-light stand, bright field, 20W Follow the safety instructions given on page 4. 왘 Fit the side-faced column to the transmitted-light base as described on page 16. 왘 Fit the microscope carrier and the stereomicroscope as described on page 16. Fitting the bulb

Fitting the graticule

Never touch the bulb when it is hot. You may sustain burns.

왘 Unscrew the sleeve from beneath the eyepiece. 왘 Push the graticule in mount over the sleeve, ensuring that it seats properly.

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왘 Screw the sleeve into position again.

Do not touch the 6V/20W halogen bulb directly with your fingers, because fingermarks can cause it to crack when heated. Remove fingermarks immediately with a cloth dipped in pure alcohol. 왘 Insert the slider together with the bulb socket.

왘 Insert the eyepiece fully into the eyepiece tube.

왘 Hold the new bulb with a cloth and insert it fully into the mount.

왘 Looking into the eyepiece, turn the eyepiece in the eyepiece tube to align the graticule, and tighten the clamping screw.

왘 Push the slider carefully but completely back along the rail to the stop. Filters

The dioptric adjustment, and setting for parfocality when a graticule is in the eyepiece, are described in the user manuals for photomicrographic and measuring systems.

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The transmitted-light stand contains a KG1 filter.

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You can insert other 50mm diameter filters if you need them: 왘 Pull out the slider. 왘 Insert the 50mm diameter filter. 왘 Push the slider carefully but completely back along the rail to the stop.

Starting up The ex-factory setting of the voltage selector is 230 (for voltages between 200–240V) and the fuse holder contains two 160mAT fuses. For 100–120V power supply: 왘 Set the voltage selector to 115. 왘 Fit the enclosed two 315mAT fuses in the fuse holder instead of the two 160mAT fuses (see section “Changing fuses”, page 21). 왘 Connect the power cable to the socket of the base and to the power source. 20