M125, M-165 and M-205 User Manual

General Instructions Safety concept Before using your microscope for the first time, please read the "Safety concept" brochure included with your instrument. It contains additional information about handling and care.

Use in clean rooms The Leica M series can be used in clean rooms without any problems. Cleaning ★★ Do not use any unsuitable cleaning agents, chemicals or techniques for cleaning. ★★ Never use chemicals to clean colored surfaces or accessories with rubberized parts. This could damage the surfaces, and specimens could be contaminated by abraded particles.

Servicing ★★ Repairs may only be carried out by Leica Microsystems-trained service technicians. Only original Leica Microsystems spare parts may be used.

Responsibilities of person in charge of instrument ★★ Ensure that the Leica stereomicroscope is operated, maintained and repaired by authorized and trained personnel only.

★★ In most cases, we can provide special solutions on request. Some products can be modified, and we can offer other accessories for use in clean rooms.

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Safety concept The individual modules of the Leica M stereomicroscopy series include an interactive CD-ROM with all relevant user manuals in 20 other languages. Keep it in a safe place, and readily accessible to the user. User manuals and updates are also available for you to download and print from our web site at www.stereomicroscopy.com. This operating manual describes the special functions of the individual modules of the Leica M stereomicroscopy series and contains important instructions for their operational safety, maintenance, and accessories.

You can combine individual system articles with articles from external suppliers (e.g. cold light sources, etc.). Please read the user manual and the safety requirements of the supplier. Before installing, operating or using the instruments, read the user manuals listed above. In particular, please observe all safety instructions. To maintain the unit in its original condition and to ensure safe operation, the user must follow the instructions and warnings contained in these user manuals.

The "Safety concept" booklet contains additional safety information regarding the service work, requirements and the handling of stereomicroscope, accessories and electrical accessories as well as general safety instructions.

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Symbols used Warning of a danger This symbol indicates especially important information that must be read and complied with. Failure to comply can cause the following:

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★★ Hazards to personnel ★★ Functional disturbances or damaged instruments

Warning of hazardous electrical voltage This symbol indicates especially important information that, if not observed, can cause the following: ★★ Hazards to personnel ★★ Functional disturbances or damaged instruments.

Leica M series

Danger due to hot surface This symbol warns against touching hot surfaces, e.g. those of light bulbs.

Important information This symbol indicates additional information or explanations that intend to provide clarity.

Action ★★ This symbol refers to actions described in the text that are to be carried out.

Figures (1) Numbers in brackets within the descriptions relate to the figures and the items within those figures.

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Safety regulations Description The individual modules fulfill the highest requirements for observation and documentation of Leica stereo microscopes of the M series.

non-Leica components that are outside of the scope of this manual.

Requirements for the owner/operator ★★ See “Safety concept” booklet

Unauthorized alterations to the instrument or noncompliant use shall void all rights to any warranty claims!

Ensure that: ★★ The M series stereomicroscopes and accessories are operated, maintained and repaired by authorized and trained personnel only.

Designated use ★★ See “Safety concept” booklet Location of use ★★ See “Safety concept” booklet Non-intended use ★★ See “Safety concept” booklet

★★ Set up the electrical components at least 10 cm from walls and combustible objects.

Never use M series microscopes or their components for surgical procedures (e.g. on the eye) unless they are specifically intended for that purpose.

★★ Avoid large temperature fluctuations, direct sunlight and vibrations. These conditions can distort measurements and micrographic images.

The devices and accessories described in this operating manual have been tested for safety and potential hazards. The responsible Leica affiliate must be consulted whenever the device is altered, modified or used in conjunction with

★★ In warm and warm-damp climatic zones, the individual components require special care in order to prevent the build up of fungus.

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★★ All operators have read, understood and observe this user manual, and particularly the safety instructions.

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Safety regulations (continued) Repair, service work ★★ See “Safety concept” booklet ★★ Only original Leica Microsystems spare parts may be used.

Installation in third-party products ★★ See “Safety concept” booklet

Disposal ★★ See “Safety concept” booklet

★★ Before opening the instruments, switch off the power and unplug the power cable. ★★ Touching the live circuit can cause injury.

Transport ★★ Use the original packaging for shipping or transporting the individual modules of the Leica M stereomicroscopy series and the accessory components.

Legal regulations ★★ See “Safety concept” booklet

EC Declaration of Conformity ★★ See “Safety concept” booklet

★★ Optimal arrangement of workplace, work assignments and work flow (changing tasks frequently). ★★ Thorough training of the personnel, giving consideration to ergonomic and organizational aspects.

★★ In order to prevent damage from vibrations, disassemble all moving parts that (according to the user manual) can be assembled and disassembled by the customer and pack them separately.

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Health risks Workplaces equipped with stereomicroscopes facilitate and improve the viewing task, but they also impose high demands on the eyes and holding muscles of the user. Depending on the duration of uninterrupted work, asthenopia and musculoskeletal problems may occur. For this reason, appropriate measures for reduction of the workload must be taken:

The ergonomic design and construction of the Leica M stereomicroscopy series are intended to reduce the exertion of the user to a minimum.

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Safety regulations (continued) Direct contact with eyepieces can be a potential transmission path for bacterial and viral infections of the eye. The risk can be kept to a minimum by using personal eyepieces for each individual or detachable eyecups.

Light sources: safety instructions Protective measures of the manufacturer: ★★ UV protection screen in front of the specimen plane prevents the user from looking directly into the UV rays. ★★ Dummy filter carriers in the free positions of the rapid filter changer prevent direct UV radiation from reaching the eyes. ★★ UV filters are installed in the observation beam paths to protect the eyes.

Warning UV radiation could damage the eyes. Therefore: ★★ Never look into the light spot on the specimen plane without a UV protection screen. ★★ Never look into the eyepieces if no excitation filter is in the beam path. ★★ Fill empty filter positions with dummy filter carriers (e.g. M165FC). ★★ Do not select a white, strongly reflective background for the stage.

★★ The stray-light protection on the lamp housing prevents irradiation of the hands.

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Safety regulations (continued) Supply unit Always unplug the supply unit from the power supply: ★★ When installing and disassembling the lamp housing ★★ Before opening the lamp housing ★★ When replacing the high-pressure mercury lamp and other parts, such as the heat-absorbing filter or the collector

Lamp housing ★★ Never open the lamp housing while the lamp is switched on. Risk of explosion, UV exposure, blinding! ★★ Before opening the lamp housing, allow it to cool off for at least 15 minutes. Danger of explosion! ★★ Never cover the air duct on the lamp housing. Danger of fire!

★★ during maintenance work on the supply unit.

Mercury lamp ★★ Follow the user manual and safety instructions provided by the lamp manufacturer, and, in particular, the section on how to proceed if a lamp breaks and releases mercury. ★★ For transport, remove the mercury lamp, transport it in its original packaging and protect moving parts in the lamp housing using the transport anchors. ★★ To minimize the risk of explosion, replace a discoloured mercury lamp promptly when it has reached the end of its rated life (follow the manufacturer's specifications and the minute meter on the supply unit). ★★ Leica Microsystems assumes no liability for damage caused by exploding, incorrectly installed or improperly used mercury lamps.

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Table of Contents General Instructions Safety concept Symbols used Safety regulations

2 3 4 5

Transmitted-light Base TL BFDF: Before First Use Transmitted-light Base TL BFDF TL RC™ / TL RCI™ IsoPro™ Manual Cross-stage: Assembly IsoPro™ Motorized Cross-stage: Assembly Cables: Terminals Cables: Cable Duct Cables: Diagram Leica LED5000 MCI™ Leica LED5000 MCI™: Alternative Assembly Leica LED5000 RL

30 31 32 33 36 40 41 42 43 44 45

The Leica M Series Congratulations! A Step Towards Infinity The Electronics: Comfort, Convenience and Safety The Modular Design: Everything is Relative Maximum Compatibility On We Go

13 14 15 16 17 18

Assembly Base and Focusing Column Optics carrier Tube Eyepieces Objective Objective Nosepiece – Assembly Objective Nosepiece – Adjusting Parfocality AX Carrier – Preparation AX Carrier – Assembly Transmitted-light Base TL ST

20 21 22 23 24 25 26 27 28 29

Quick Start Guide The Fastest Route to Success Overview of an M series Microscope The Correct Interpupillary Distance Using the Eyepieces Focusing Adjusting the resistance of the focus drive Changing the magnification (zoom) Ratchet Steps and Magnification Levels Parfocality: More Comfort and Convenience for Your Work Iris Diaphragm

47 48 49 50 51 52 53 54 55 56

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Eyepieces Magnification Factors of the Eyepieces Health Notes Dioptric correction Dioptric Correction and Parfocality Graticules

58 59 60 61 62

Photography & Video Photography & Video Photo tubes and C-mounts Trinocular video/phototube 50% Trinocular video/phototube 100%

64 65 66 67

Microscope Carrier The Microscope Carrier AX The Objective Nosepiece

69 71

Bases Transmitted-light Base TL ST: Controls Transmitted-light Base TL ST: Operation Transmitted-light Base TL ST: Lamp Replacement Transmitted-light Base TL BFDF: Controls Transmitted-light Base TL BFDF: Operation TL RC™ / TL RCI™: Controls TL RCI™: The Deflection Mirror TL RCI™: Color Intensity and Temperature TL RC™ / TL RCI™: Operation TL RCI™: Methods in Transmitted Light TL RCI™: Relief Images Using Filters IsoPro™ (Non-motorized): Controls IsoPro™ (Motorized): Controls

75 76 77 78 79 80 81 82 83 84 85 87 88 89

System Illumination Leica LED5000 MCI™ Leica LED5000 RL

91 93

Objectives andoptical accessories The Different Types of Objectives

73

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Accessories Leica PSC Controller

95

Dimensional Drawings Leica M125 / M165 C Leica M205 C Leica LED5000 MCI Leica LED5000 RL

97 101 104 105

Specifications for the Bases Transmitted-light Base TL ST Transmitted-light Base TL BFDF TL RC™ / TL RCI™ IsoPro™ Motorized XY Stage

107 108 109 110

Appendix Calculating the Total Magnification and Field of View Diameter Care, Maintenance, Contact Persons

112 113

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Congratulations! Congratulations on obtaining your new Leica M series stereomicroscope. We are convinced it will exceed your expectations, as never before have we applied our decades of experience in the areas of optics, mechanical engineering and ergonomics in such an uncompromising manner. The M series embodies all the qualities you associate with the name Leica Microsystems: excellent objectives, high-quality engineering, and reliability. Furthermore, the modular design ensures that the M series adapts perfectly to your needs-no matter which accessories you require for your tasks.

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Though the reliability and robustness of Leica stereomicroscopes is legendary, like any high-tech product, the M series requires a certain degree of care and attention. Therefore, we recommend that you read this manual. It contains all the information you need regarding operation, safety and maintenance. Simply observing a few guidelines will ensure that even after years of intensive use, your stereomicroscope will continue to work as smoothly and reliably as on the very first day. We wish you the best of success in your work-after all, you are now equipped with the best tool!

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A Step Towards Infinity Ever since their introduction by Horatio S. Greenough, stereomicroscopes have worked according to the optical principles based primarily on Ernst Abbe's research. For over a century, ingenious optics designers and engineers have worked to push magnification, resolution and image fidelity to the limit permitted by optics. In doing so, they have always been constrained by the interrelation between three factors: the higher a microscope's resolution, the lower the available working distance. If one increases the distance of the optical axes, the three-dimensional image seen by the observer becomes distorted-a sphere becomes an ellipse, a flat surface curves toward the observer.

Limits are made to be broken. The Leica M205 C is the world's first stereomicroscope with a zoom range of 20.5:1. This accomplishment, however, was not enough for Leica's engineers. With the new FusionOptic™ in the M205 C, they have succeeded in going yet another step beyond previous limits. In addition to the increase in magnification, the resolution, too, has been increased to up to 1050 lp / mm*, which corresponds to a resolved structure size of 472 nm. Of course, this performance increase benefits your everyday work. Set up your specimens on the microscope with comfortable freedom of movement and discover details in stereomicroscopy that you could never see before.

* Leica M205 C with 1.0× planapochromat and 10× eyepieces

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The Electronics: Comfort, Convenience and Safety for your Experiments Never before have electronics been used as extensively in a Leica series as in the new M series. Optics carrier, tripod, base and illuminator are all connected using electrical contacts-which provides a number of advantages.

Reliability for your experiments Exact reproducibility of results is an increasingly common demand, particularly in research and development. The continuous encoding captures parameters such as the magnification, the illumination, the position of the iris

Contacts not only transmit data, but also supply the power.

Leica Application Suite (LAS) evaluates the transmitted data and can restore the test situation later.

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diaphragm and more to the Leica LAS software. Thus you always know the conditions under which an image was acquired. Fewer cables A large part of the cables have been routed in the interior of the column. Data are also transferred via the interface between the column, the optics carrier and the carrier. The direct result is that you need fewer cables-this not only makes your workstation neater and more comfortable, it even makes it appear larger.

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The Modular Design: Everything is Relative The Leica M series provides maximum flexibility in choosing equipment, thanks primarily to the modular configuration and the compatibility that Leica has painstakingly maintained for decades. The optics carriers, eyepieces, bases and more can be combined in any way you choose, allowing you to create the microscope that best suits your needs.

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Despite this, you will notice that the controls and individual components do not differ significantly. Whichever configuration you choose, you will quickly feel right at home.

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Have a special request? Let us know! Leica Microsystems enjoys an exceptional reputation when it comes to devising customerspecific solutions. If you have a special request that cannot be met with standard parts, contact your Leica consultant. We have a solution for every problem.

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Maximum Compatibility Leica engineers were careful to ensure that the new M series-like its predecessors-remains compatible with existing series. This means that objectives, bases, tubes and so on can be reused. Objectives All new objectives of the M series are parfocal, meaning that when used with the objective nosepiece, they can be replaced while keeping the specimen in perfect focus. If you prefer, you can continue to use the previous objective series. In this case, parfocality is no longer guaranteed.

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Tubes The interface between the optics carrier and the tube has remained the same, so existing tubes fit the new M series. The new tubes are designed for eyepieces with field number 23, while the predecessor models were only designed for field number 21, resulting in a smaller object field. Eyepieces However, the new M-series eyepieces models have an audible and tangible click to provide immediate feedback in case of accidental adjustment.

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On We Go If your new Leica microscope has already been assembled and commissioned by your Leica consultant, click here to skip through the installation instructions and go directly to the Quick Start Guide on page 46.

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If, on the other hand, you are assembling the microscope yourself, continue with the "Assembly" chapter, which begins on page 19.

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Base and Focusing Column The first step is to connect the focusing column of the M series to the corresponding base.

Assembling the column adapter 1. Securely install the column adapter on the column using the four included screws.

Assembling the focusing drive 2. Securely screw the focusing column to the base using the six included screws.

Tools used ★★ Hex socket screwdriver, 3 mm

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Optics carrier Tools used ★★ Hex socket screwdriver, 4 mm

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Assembling the optics carrier 1. Place the optics carrier on the focusing column so that the screw fit into the thread provided and the lug fits into the groove.

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2. Press the optics carrier backwards to the focusing column and screw it in place using your other hand.

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Tube All intermediate tubes that fit between the optics carrier and the binocular tube are fitted in the same manner. Tools used ★★ No tools required.

Assembling the tube 2. Push the tube (for example, the inclined binocular tube) into the dovetail ring and rotate it slightly in both directions until the positioning screw meshes with the guide groove.

3. While holding the tube only slightly, carefully tighten the positioning screw. It is automatically brought to the correct position.

Preparations 1. Unscrew the positioning screw and remove the protective cover.

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