Leica Microsystems
TCS SP5 User Manual
User Manual
166 Pages
Preview
Page 1
Leica TCS SP5 User Manual
Table of Contents
1
Notes about this manual...
page 1
1.1
Copyright...
page 1
2
General...
page 3
2.1
About these operating instructions...
page 3
3
Proper intended use...
page 5
4
Legal Notes...
page 7
4.1
DISCLOSURE...
page 7
4.2
REVISIONS AND CHANGES...
page 8
4.3
WARRANTY...
page 8
4.4
Patents...
page 8
4.5
TRADEMARKS...
page 10
4.6
Software licenses...
page 10
5
TCS SP5 specifications...
page 11
5.1
System overview...
page 11
5.2
Dimensions...
page 12
5.2.1
System with inverted stand:...
page 12
5.2.2
System with upright stand...
page 13
5.3
Installation site requirements...
page 13
5.4
Permissible ambient conditions...
page 14
5.5
Electrical connections...
page 14
5.6
Required cooling capacity...
page 16
5.7
Important additional notes...
page 16
5.8
Required safety measures for VIS and UV systems...
page 17
5.9
Required laser safety measures for MP systems...
page 17
5.10
Important additional notes...
page 17
6
Safety Notes...
page 19
6.1
Which standards does this product meet?...
page 19
6.2
Which laser class does the product have?...
page 21
6.3
Laser class for VIS and UV systems...
page 21
6.4
Laser class for MP systems...
page 21
6.5
Warnings, Safety Cautions, and Notes...
page 22
6.6
What should the user of the laser scanning microscope observe?
page 23
6.7
Safety Notes for the User...
page 25
User Manual TCS SP5 V: 02 | Document-ID-No.:156500002
Table of Contents
6.8
Safety Notes for Operation...
page 26
6.9
Specific Safety Notes...
page 30
6.9.1
Operational reliability...
page 30
6.9.2
De-energizing the system...
page 30
6.9.3
Maximum current load of the power outlet strip at the supply unit
page 31
6.10
Laser safety devices...
page 32
6.10.1
Eye protection for VIS or UV systems...
page 32
6.10.2
Detachable-key switch...
page 33
6.10.3
Emissions warning indicators...
page 34
6.10.4
Remote interlock connector on the supply unit...
page 36
6.10.5
Remote interlock connector on the external 405nm advanced laser
page 37
6.10.6
Remote interlock connectors on additional external lasers...
page 37
6.10.7
Interlock connection on scanner...
page 39
6.10.8
Function and position of safety switches...
page 40
6.10.9
Transmitted-light lamp housing for inverted stands...
page 40
6.10.10
Transmitted-light lamp housing for upright stands...
page 42
6.10.11
Mirror housing on upright stand...
page 43
6.10.12
Special laser safety equipment...
page 44
6.10.12.1 Safety beam guide and beam collector...
page 44
6.10.12.2 Eye protection for MP systems...
page 45
6.10.12.3
Shielding in MP systems (UV laser)...
page 46
6.11
Overview of usable VIS/UV lasers...
page 47
6.12
Overview of usable MP lasers (IR)...
page 48
6.13
Safety label on TCS SP5 system...
page 49
6.13.1
Inverted stand DMI 4000/6000 CS...
page 49
6.13.2
Upright stand DM 5000/6000 CS...
page 50
6.13.3
Scan head...
page 53
6.13.4
Supply unit...
page 54
6.13.5
External 405-nm laser Advance / 405-nm imaging laser...
page 55
6.13.6
External 488-nm laser...
page 56
6.13.7
MP beam coupling unit...
page 57
6.13.8
Cover (for replacement flange)...
page 58
6.14
Requirements related to the installation/storage location...
page 59
6.15
Changing the installation site...
page 60
6.16
Scanner cooling...
page 61
7
Care and cleaning...
page 63
7.1
Cleaning the optical system of the microscope...
page 63
7.2
Cleaning the microscope surface...
page 64
User Manual TCS SP5 V: 02 | Document-ID-No.:156500002
Table of Contents
8
Startup of the system...
page 67
8.1
Setting Up Users...
page 70
9
Starting the LAS AF...
page 75
10
Introduction to LAS AF - Help...
page 79
10.1
General...
page 79
10.1.1
Calling Online Help...
page 79
10.1.2
Structure of the online help...
page 80
10.2
Imprint...
page 81
10.2.1
In the respective context (context-sensitive)...
page 82
10.2.2
Via the Help menu...
page 82
10.2.3
Full-text search with logically connected search terms...
page 83
10.3
Key combinations...
page 84
11
Structure of the user interface...
page 85
11.1
General structure of the user interface...
page 85
12
What is the Köhler illumination?...
page 87
12.1
Setting the Köhler Illumination...
page 88
13
Introduction to confocal work...
page 91
13.1
Preparation...
page 91
13.1.1
The objective...
page 92
13.1.2
Conventional microscopy...
page 93
13.1.3
Why scan?...
page 96
13.1.4
How is an optical section created?...
page 98
13.2
Acquiring optical sections...
page 100
13.2.1
Data acquisition...
page 101
13.2.2
Illumination...
page 103
13.2.3
Beam splitting...
page 104
13.2.4
Emission bands...
page 104
13.2.5
The pinhole and its effects...
page 106
13.2.6
Image detail and raster settings...
page 108
13.2.7
Signal and noise...
page 113
13.2.8
Profile cuts...
page 116
13.3
Multiparameter fluorescence...
page 117
13.3.1
Illumination...
page 117
User Manual TCS SP5 V: 02 | Document-ID-No.:156500002
Table of Contents
13.3.2
Beam splitting...
page 118
13.3.3
Emission bands...
page 119
13.3.4
Crosstalk...
page 119
13.3.5
Sequential capture...
page 121
13.3.6
Unmixing...
page 121
13.4
3D Series...
page 124
13.4.1
z-stack...
page 124
13.4.2
About section thickness...
page 124
13.4.3
...and spacing...
page 125
13.4.4
Data volumes...
page 125
13.4.5
Depictions...
page 127
13.4.5.1
Gallery...
page 127
13.4.5.2
Movie...
page 128
13.4.5.3
Orthogonal projections...
page 128
13.4.5.4
Rotated projections...
page 129
13.5
Time series...
page 131
13.5.1
About scan speeds...
page 131
13.5.2
Points...
page 132
13.5.3
Lines...
page 132
13.5.4
Planes...
page 132
13.5.5
Spaces (time-space)...
page 133
13.5.6
FRAP measurements...
page 133
13.6
Spectral series...
page 134
13.6.1
Data acquisition and utilization...
page 134
13.6.2
About spectral resolution...
page 134
13.7
Combinatorial analysis...
page 135
5
Switching off the system...
page 137
6
Contact...
page 139
7
Declaration of conformity...
page 141
8
Glossary...
page 143
18
Safety data sheets...
page 153
User Manual TCS SP5 V: 02 | Document-ID-No.:156500002
Notes about this Manual
1
Notes about this manual
1.1
Copyright The contents of this manual are current as of the time of publication and are subject to change without notice. Leica Microsystems CMS GmbH assumes no warranty with respect to this manual, including but not limited to implicit warranties for salability and for suitability for a specific purpose. Leica Microsystems CMS GmbH is not liable for errors or accidental damages or incidental damages in conjunction with equipment, performance or usage of this manual or the examples contained therein. © Leica Microsystems CMS GmbH This document contains information that is protected by copyright law. All rights reserved. Duplication, adaptation or translation of this manual requires the prior written approval of Leica Microsystems CMS GmbH unless otherwise permitted through copyright arrangements. The programs for controlling this product are protected by copyright law. All rights reserved. Reproduction, adaptation or translation of these programs without prior written approval of Leica Microsystems CMS GmbH is strictly prohibited. Microsoft Windows, Windows XP, the Windows logo and the Windows CE logo are ei-
User Manual TCS SP5 V: 02 | Document-ID-No.: 156500002
Page 1
Notes about this Manual
ther registered trademarks or trademarks of Microsoft Corporation in the US and/or other countries. Microsoft products are licensed for other companies by Microsoft Licensing, Inc., a subsidiary company that is wholly owned by Microsoft Corporation. All other tradenames and product names in this document are brands, service brands, trademarks or registered trademarks of their respective owners.
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User Manual TCS SP5 V: 02 | Document-ID-No.: 156500002
General
2
General
2.1
About these operating instructions The main focus of these operating instructions is directed to the safety instructions that must be observed while working with the laser scanning microscope. In addition, these operating instructions provide a rough overview of the operating principle of laser scanning microscopes. It presents you with the first steps for starting and startup of the system and provides a description of the Leica Application Suite Advanced Fluorescence (LAS AF). The Leica TCS SP5 is supplied with the latest version of the licensed Leica Application Suite Advanced Fluorescence. To maintain information on the most current level, the description of software functions was intentionally omitted from these operating instructions. Instead, you are referred to the online help of the Leica Application Suite Advanced Fluorescence in which you can obtain the most up-to-date explanations and instructions about the corresponding software functions. Please read the chapter ”Introduction to the help of the Leica Application Suite Advanced Fluorescence” in these operating instructions to familiarize yourself first with setup and operation. Additional information about specific functions can subsequently be obtained electronically at your TCS workstation.
User Manual TCS SP5 V: 02 | Document-ID-No.: 156500002
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General
Page 4
User Manual TCS SP5 V: 02 | Document-ID-No.: 156500002
Proper Intended Use
3
Proper intended use The system was designed for confocal recording (laser scanning images) of fluorescence-marked living and fixed specimens as well as for quantitative measurements in the area of life science. This system is intended for use in a lab. Applications of in-vitro diagnostics according to the medical products law are excluded from proper intended use. The manufacturer assumes no liability for any improper intended use and for use outside the specifications of Leica Microsystems CMS GmbH as well as any risks resulting from it. In such cases, the declaration of conformity becomes invalid.
User Manual TCS SP5 V: 02 | Document-ID-No.: 156500002
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Proper Intended Use
Page 6
User Manual TCS SP5 V: 02 | Document-ID-No.: 156500002
Legal Notes
4
Legal Notes Manufactured in the Federal Republic of Germany. © Copyright 2005, Leica Microsystems CMS GmbH. All rights reserved. No part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording, or storing in a retrieval system, or translating into any language in any form without the express written permission of Leica Microsystems CMS GmbH. This also includes photocopying, recording or storing on a retrievable system as well as the translation into another language.
4.1
DISCLOSURE This document contains Leica Microsystems CMS GmbH proprietary data and is provided solely to its customers for their express benefit of safe, efficient operation and maintenance of the product described herein. Use or disclosure of Leica Microsystems CMS GmbH proprietary data for the purpose of manufacture or reproduction of the item described herein, or any similar item, is prohibited, and delivery of this document shall not constitute any license or implied authorization to do so.
User Manual TCS SP5 V: 02 | Document-ID-No.: 156500002
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Legal Notes
4.2
REVISIONS AND CHANGES Leica Microsystems CMS GmbH reserves the right to revise this document and/or to further develop and improve the products described in this document at any time without prior notice or any other obligation. Information and specifications in this manual are subject to change without notice.
4.3
WARRANTY Leica Microsystems CMS GmbH provides this publication ”as is” without warranty of any kind, either expressed or implied, including but not limited to the implied warranties of merchantability or fitness for a particular purpose. All reasonable precautions have been taken in the preparation of this document, including both technical and non-technical proofing. Leica Microsystems CMS GmbH accepts no responsibility for any errors or omissions. In addition, Leica Microsystems CMS GmbH is not responsible for direct, incidental or consequential damages that could result from the use of the material described in this document.
4.4
Patents The product is protected by the following US patents: 5,886,784; 5,903,688; 6,137,627; 6,222,961;
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User Manual TCS SP5 V: 02 | Document-ID-No.: 156500002
Legal Notes
6,285,019; 6,311,574; 6,355,919; 6,423,960; 6,433,814; 6,444,971; 6,466,381; 6,510,001; 6,614,526; 6,654,165; 6,657,187; 6,678,443; 6,687,035; 6,738,190; 6,754,003; 6,801,359; 6,831,780; 6,850,358; 6,867,899. Further patents are pending.
User Manual TCS SP5 V: 02 | Document-ID-No.: 156500002
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Legal Notes
4.5
TRADEMARKS Throughout this manual, trademarked names may be used. Rather than including a trademark (™) symbol at every occurrence of a trademarked name, we state that we are using the names only in an editorial fashion, and to the benefit of the trademark owner, with no intention of infringement.
4.6
Software licenses The software described in this document is furnished under a License Agreement which is included with the product. This agreement specifies the permitted and prohibited uses of the product.
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User Manual TCS SP5 V: 02 | Document-ID-No.: 156500002
Specifications
5
TCS SP5 specifications
5.1
System overview
1
6
figure1.
2
5
4
3
System components (overview)
1 TCS SP5 Scanner 2 Control panel 3 TCS workstation 4 Supply unit 5 Panel box 6 Stand with scanner
User Manual TCS SP5 V: 02 | Document-ID-No.: 156500002
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Specifications
5.2
Dimensions
5.2.1
System with inverted stand:
figure2.
Page 12
Dimensions of the TCS SP5 with inverted stand
User Manual TCS SP5 V: 02 | Document-ID-No.: 156500002
Specifications
5.2.2
System with upright stand
figure3.
Dimensions of the TCS SP5 with upright stand
5.3
Installation site requirements
Do not expose the system to draft. Ensure that the system is not installed next to elevators, air conditioners or ventilation systems. For this reason, the installation site should be carefully planned. Ensure that the environment is as dustfree as possible.
User Manual TCS SP5 V: 02 | Document-ID-No.: 156500002
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Specifications
Please also read the notes on protection against dust in the chapters on maintenance and cleaning. Installing the system in darkened rooms is also advisable. For installation, maintenance and transport, the TCS SP5 system requires doors with inside spans of 1.00 m. With regard to the load-bearing capacity of the floor, please note that the system will apply a static load of 200 kg/m@.
5.4
Permissible ambient conditions Permissible temperature range for operation: 18 ... 25° C Permissible relative humidity: 20-80% (noncondensing) Permissible vibration speeds: Frequency range [5 Hz–30 Hz]: less than 30 μm/s (effective value) Frequency range [> 30 Hz]: less than 60 μm/s (effective value) Pollution degree: Class 2
5.5
Electrical connections Supply voltage (Input 1/Input 2): 100...240 V AC +/- 10% Frequency: 50/60 Hz Power consumption: 2 x 1.6kW Overvoltage category: II The building installation must feature two separate power connections with the following fuse protection: D
Page 14
100 V - 120 V AC power: 20 A
User Manual TCS SP5 V: 02 | Document-ID-No.: 156500002
Specifications
D
200 V - 240 V AC power: 12-16 A
For the specifications of external lasers such as UV and MP lasers, please refer to the manufacturer’s documentation.
User Manual TCS SP5 V: 02 | Document-ID-No.: 156500002
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