TP1020 Instruction Manual V2.4 Aug 2009

1. Important information The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and stateof-the-art technology as we understand it following thorough investigation in this field. We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to provide our customers with additional copies, updates etc. of this manual. For erroneous statements, drawings, technical illustrations etc. contained in this manual we exclude liability as far as permissible according to the national legal system applicable in each individual case. In particular, no liability whatsoever is accepted for any financial loss or consequential damage caused by or related to compliance with statements or other information in this manual. Statements, drawings, illustrations and other information as regards contents or technical details of the present manual are not to be considered as warranted characteristics of our products. These are determined only by the contract provisions agreed between ourselves and our customers.

Leica reserves the right to change technical specifications as well as manufacturing processes without prior notice. Only in this way is it possible to continuously improve the technology and manufacturing techniques used in our products. This document is protected under copyright laws. Any copyrights of this document are retained by Leica Biosystems Nussloch GmbH. Any reproduction of text and illustrations (or of any parts thereof) by means of print, photocopy, microfiche, web cam or other methods – including any electronic systems and media – requires express prior permission in writing by Leica Biosystems Nussloch GmbH. For the instrument serial number and year of manufacture, please refer to the name plate at the back of the instrument. © Leica Biosystems Nussloch GmbH

Published by: Leica Biosystems Nussloch GmbH Heidelberger Str. 17 - 19 D-69226 Nussloch Germany Phone: +49 (0)62 24 143-0 Fax: +49 (0)62 24 143-2 68 e-Mail: [email protected] Internet: http://www.leica-microsystems.com Leica TP1020

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Table of contents 1.

Important information ... 3

2.

Safety ... 9

3.

Instrument components and specifications ... 12

3.1 3.2 3.3 3.3 3.4 3.5 3.5.1 3.5.2 3.5.3 3.5.4

Technical Data ... 12 Description of the instrument ... 14 Standard delivery ... 15 General overview Leica TP1020 - Type 1 with additional paraffin station (optional accessory) ... 16 General overview Leica TP1020 - Type 3 ... 18 Accessories supplied together with the instrument ... 20 Standard tissue basket ... 20 Glass beaker with holder ... 20 Aluminum container with holder ... 21 Paraffin station ... 22

4.

Setting up the instrument ... 23

4.1 4.1 4.2 4.3 4.3.1 4.3.4 4.3.3 4.4 4.5 4.6 4.6.1 4.6.2 4.6.3 4.6.4

Unpacking ... 23 Unpacking (continuance) ... 24 Requirements at the installation site ... 25 Electrical connection ... 26 Checking the voltage selector setting ... 26 Adjust the voltage selector ... 27 Connecting the mains cable ... 28 Switching on the instrument ... 29 Setting the time ... 29 Installing the accessories ... 30 Installing the paraffin stations ... 30 Connecting the paraffin stations ... 31 Fixing the paraffin station connection cables (instruments with fume control system only) ... 32 Installing the reagent stations ... 32

5.

The user interface ... 33

6.

Programming the instrument ... 39

6.1 6.1.1 6.2 6.2.1 6.2.2 6.2.3 6.2.4 6.2.5 6.2.6

General description ... 39 Special characteristics of two-basket programs ... 40 Setting-up / editing programs ... 41 Selecting programming mode ... 41 Selecting the program ... 41 Selecting the number of baskets ... 41 Selecting the starting station ... 42 Activating the vacuum function (instruments with vacuum function only) ... 42 Selecting infiltration times per station ... 43

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Instruction manual V 2.4 – 08/2009

Table of contents 6.2.7 9.2.8

Displaying total program duration ... 44 Leaving the programming mode ... 44

7.

Preparations for tissue processing ... 45

7.1 7.2 7.3

Filling the reagent stations ... 45 Filling the paraffin stations ... 46 Inserting the tissue basket ... 48

8.

Operating the instrument in the manual processing mode ... 50

8.1 8.2 8.3

Lifting and lowering the tissue basket ... 50 Moving the tissue basket to the next station ... 51 Activating the vacuum function (instruments with vacuum function only) ... 52

9.

Operating the instrument in the automatic processing mode ... 53

9.1 9.1.1 9.1.2 9.2 9.3 9.4 9.5 9.6 9.7 9.8 9.9

Starting a program ... 53 Immediate start ... 53 Delayed start ... 55 Editing and altering programs while a processing cycle is in progress ... 59 Display end of processing time ... 59 Pausing a process ... 60 Resuming a paused process ... 61 Stopping or aborting a process ... 61 End of an automatic process ... 62 Remove the specimens ... 63 Finishing your daily work ... 63

10.

Warning and error codes - troubleshooting ... 64

10.1 Warning codes ... 64 10.2 Error codes ... 66 10.3.1 ’POWER FAILURE’ and ’WRONG STATION’ messages ... 68 10.3.2 Measures to remove specimen material in case of prolonged power failure ... 69 10.4 ‘ABORT’ message ... 71

11.

Cleaning ... 72

12.

Maintenance ... 74

12.1 Allgemeine Wartungshinweise ... 74 12.2 Paraffin stations ... 75 12.2.1 Altering the standard working temperature ... 75 12.2.2 Reset after excess temperature shutdown ... 76 12.3 Replacing the fuses ... 77

13.

Optional accessories ... 78

13.1 13.2

Three-level tissue basket ... 78 Basket removal device ... 79

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13.3 13.4 13.5

Basket holder for second tissue basket ... 80 Connecting the fume outlet tube (optional accessory for instruments with fume control system) ... 83 Inserting the activated carbon filters (optional on instruments with fume control system) ... 84

14.

Ordering information ... 85

14.1 14.2

TP 1020 Automatic tissue processor ... 85 Accessories ... 86

15.

Appendix ... 87

15.1 15.2 15.4

Altering the instrument standard configuration ... 87 Reagents appropriate for use with the instrument ... 90 Factory-set programs ... 91

16.

Warranty and Service

17.

EC-Declaration of Conformity ... 96

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Instruction manual V 2.4 – 08/2009

... 95

1. Symbols used in this manual and their meaning Warnings appear in a grey box and are marked by a warning triangle .

Notes, i.e. important user information appear in a grey box and are marked by an information symbol .

(5)

Figures in brackets refer to item nos. in drawings.

ENTER

Function keys to be pressed on the instrument touch screen are written in bold-print capital letters.

Qualification of personnel • The Leica TP1020 may be operated only by trained laboratory personnel. • The instrument may be operated only according to the instructions contained in this manual.

Important Information

The Leica TP1020 is a modular automated tissue processor designed for the following laboratory applications: • fixation • dehydration • paraffin wax infiltration of histological tissue specimens. The Leica TP1020 must be operated exclusively with the reagents listed in chapter 15.2. The instrument may be operated only according to the instructions contained in this manual. Any other use of the instrument is considered improper! Failure to adhere to these instructions may result in an accident, personal injury, damage to the instrument or accessory equipment.

Instrument type All information provided in this manual applies only to the instrument type indicated on the title page. A name plate indicating the instrument serial number is attached to the back of the instrument.

Designated use The instrument has been designed so that it is safe to use by the operator as well as for professing specimens - provided that it is operated according to the present instruction manual.

Fig. 1 Leica TP1020

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1.

Important information Make sure to comply with the safety instructions and warnings in this chapter. Make sure to read these instructions, even if you are already familiar with the operation and use of other Leica products.

Safety instructions This instruction manual includes important information related to the operating safety and maintenance of the instrument and it is an important part of the product.

If additional requirements on accident prevention and environmental protection exist in the country of operation, this instruction manual must be supplemented by appropriate instructions to ensure compliance with such requirements.

This instrument has been built and tested in accordance with the following safety regulations on electrical measuring, control, regulating and laboratory devices. In order to maintain this condition and to ensure safe operation, the operator must observe the instructions and warnings contained in this instruction manual. For current information about applicable standards, please refer to the CE declaration of conformity on our Internet site: www.leica-microsystems.com

The protective devices on both instrument and accessories may neither be removed nor modified. Only authorized and qualified service personnel may repair the instrument and access the instrument’s internal components.

Warnings The safety devices installed in this instrument by the manufacturer only constitute the basis for accident prevention. Primarily responsible for accident-free operation is above all the institution which owns the instrument and, in addition, the designated personnel who operates, services or repairs the instrument. To ensure trouble-free operation of the instrument, make sure to comply with the following instructions and warnings.

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Instruction manual V 2.4 – 08/2009

2.

Safety

Transport and installation • Keep in mind Chapter 3, 'Technical data'! • During transport, keep the instrument up-right! • When transporting the instrument, do not lift it up by the carousel cover! • This instrument may not be operated in hazardous locations! • Attention: the voltage selector is factory preset. Before connecting the instrument to themains make sure the correct setting matching your laboratory’s power supply has been selected. The socket for connecting the mains cable, located at the rear of the instrument is sealed with an adhesive tape which indicates the factory preset voltage for your particular instrument. • Connecting the instrument to the mains with the voltage selector set at a wrong value can cause severe damage to the instrument! • When adjusting the voltage selector setting, the instrument must be disconnected from the mains. • The instrument may only be connected to the mains with the cable supplied together with the instrument and it may only be connected to grounded sockets.

Operating the instrument • The carousel may not be rotated manually!Severe damage will result from doing so! • Caution when lowering the carousel! Keep your fingers out of the space between the container lid and the upper rim of the container! • Caution! In case of a power failure, thecarousel automatically descends into a station. • Instruments equipped with vacuum func-tion may only be operated with the aluminium containers supplied together with theinstrument. • While operating the instrument, no liquid may enter in contact with any of the electrical connections or the interior of the in-strument. • Make sure to observe the level indicators on the reagent and paraffin stations. • Warning! Use caution when handling solvents! Make sure the premises are adequately ventilated! Explosion hazard! • Spilled reagents have to be wiped away immediately. In case of long-term expo-sure, the instrument surfaces are only con-ditionally resistant to solvents. • Always observe worker’s protection rules and use adequate protective gear (gloves, laboratory coats).

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2.

Safety

Operating the instrument (continuance) • The heated wax baths may only be used with paraffin. Under no circumstances may they be filled with solvents. When solvents heat, a highly explosive mixture builds up! • Caution! The interior containers of the paraffin stations become very hot when the heating function is activated! Do not touch the gray upper rim of the containers with your hands! Risk of injury! • Caution when handling hot paraffin! Risk of injury!

Cleaning • Before cleaning the instrument, disconnect the mains switch. • Caution! The interior containers of the paraffin stations become very hot when the heating function is activated! Do not touch the gray upper rim of the containers with your hands! Risk of injury! • Caution when handling hot paraffin! Risk ury! • When cleaning the instrument, no liquid may enter in contact with any of the electrical connections or the interior of the instrument. • Spilled reagents have to be wiped away immediately. In case of long-term exposure, the instrument surfaces are only conditionally resistant to solvents. • To clean the painted surfaces, the container platform and the control panel, do not use solvents containing acetone or xy-lene; neither use abrasive cleaning powders!Only mild household detergents may be used!The lacquered surfaces and the control panel are not resistant to xylene or acetone!

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Instruction manual V 2.4 – 08/2009

2.

Safety

Maintenance • For purposes of maintenance or repair, the instrument may only be opened by service technicians authorized by Leica. • Before exchanging the fuses, switch off the mains switch and unplug the instrument! • Burnt-out fuses may only be replaced by fuses of the same type and specification. For appropriate brands and specifications, see Chapter 4 ‘Technical Data’.

Paraffin stations • If the excess temperature repeatedly - please call Leica Technical Service. DO NOT continue to use the paraffin station. • Check if the paraffin station works trouble-free. Use of defective paraffin stations has to be discontinued for safety reasons!

Activated carbon filter (optional accessory) • Warning! Fire hazard! It is important that the activated carbon filters are changed at factory recommended intervals. If a filter becomes saturated with solvent, there is a potential fire risk!

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3.

Instrument components and specifications

3.1

Technical Data

Type TP1020 Approvals ... UL / cUL / VDE Electrical data Nominal voltage ... 100 / 120 / 230 /240 V AC ±10% Rate frequency ... 50 - 60 Hz Mains fuses (type MDA; manufacturer: Bussmann) ... 2 x T 10 A, UL-approved Nominal capacity ... 700 VA Classifications according to IEC-1010/EN61010-1: Protective class ... I Pollution degree ... 2 Overvoltage installation category ... II Heat emission (max.) ... 700 J/s Working temperature range: ... 5 °C - 40 °C Relative humidity of air ... 80%, non-precipitating Dimensions Carousel lid ... 820 mm Ø Height ...595 - 780 mm Pitch diameter of rollers ... 610 mm Ø Weight Net weight (including accessories) ... 60 kg Net weight, including packaging material ... 116 kg Paraffin stations Quantity: ... 2 (optionally 3) Capacity: ... 1.8 l Nominal voltage / rated frequency: ... 230 V AC, 50-60 Hz Nominal capacity per station: ... 150 VA Temperature setting range: ... 45 °C - 65 °C ±3 K Excess temperature disconnection: ... 85 °C ±5 K

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Instruction manual V 2.4 – 08/2009

3.

Instrument components and specifications

Technical Data (continuance) Reagent stations Quantity: ... 10 (9 when 3rd paraffin station is installed) Capacity: ... 1.8 l Tissue basket Quantity: ... 1 (optionally 2) Capacity: ... 100 cassettes (optionally: 200) Programs Quantity: ... 9, freely selectable Programmable infiltration time per station: ... up to 99 hours 59 minutes Delayed start time of processing: ... up to 9 days Dripping time: ... 60 seconds Vacuum function (types 2 and 4) Pressure difference ... 500 hPa maximum (appr. 0.5 bar)

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3.

Instrument components and specifications

3.2

Description of the instrument The Leica TP1020 is an automatic tissue processor used for the fixation, dehydration and infiltration of histological tissue samples with fixatives, alcohol, solvents and paraffin wax. The reagent stations numbered 1 - 10 are used to contain reagents. Station 10 may be replaced with an optional third paraffin wax bath. Stations 11, 12, and if used 10, are heated, temperature controlled wax baths that can be filled with either wax pellets or molten paraffin wax. Embedding cassettes used to hold the tissue samples, are placed into the tissue basket. The basic instrument is designed for a single tissue basket. An optional second basket can be added. The basket, or baskets, are moved clockwise from station to station. To ensure thorough infiltration the basket containing the tissue samples is agitated, by moving up and down, at each station. This function can be switched off at any time. During processing as the tissue basket moves from station to station there is a delay period of sixty (60) seconds during which time the basket is suspended above the station. This ensures that there is minimal reagent carryover from station to station. All instrument functions are activated through the control panel. Real time is displayed via LCD. The instrument can be operated in manual and automatic processing mode. Automatic processing is controlled via 9 different programs which can be individually set up, altered and edited. If a power failure occurs, the specimens are protected from drying out even when overnight processing has been selected, since in case of a power failure the tissue basket will always be immersed into a station. Once mains power is restored, processing will be resumed where it had been interrupted. After a long-term power failure, critical excess immersion time in a station will be visually displayed. The Leica TP1020 has been designed to comply with the strict UL and VDE safety regulations. It is manufactured under a quality system in compliance with ISO9001.

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Instruction manual V 2.4 – 08/2009

3. 3.3

Instrument components and specifications

Standard delivery The Leica TP1020 is available in 4 different versions: Type 1 - Basic instrument Type 2 - Basic instrument with vacuum function Type 3 - Basic instrument with fume control system Type 4 - Basic instrument with vacuum function and fume control system The basic instrument comes with the following accessories: 12 glass Beakers with holder 2 paraffin containers 1 standard tissue basket 2 set of replacement fuses (2 x T 10,0 A) 1 set of mains cables -1 power cord „D“ - 1 power cord „USA-C-J“ - 1 power cord „UK“ ST/BU F-5A 1 screwdriver 5,5 x 200 1 crank handle (mounted) 1 instruction manual for Leica TP1020

14 0422 42545 14 0422 30665 14 0422 30585 14 0600 00759 14 0411 45754 14 0411 13558 14 0411 13559 14 0411 27822 14 0170 10702 14 0222 30663 14 0422 80001

Types 2 and 4 - instead of glass b+eakers, these instruments are supplied with: 10 aluminum containers with holders Vacuum function and fume control system are not available as a retrofitting option. All types can also be retrofitted with a third paraffin bath at station 10 (optional accessory). You will find the standard accessories and - in case you ordered them - further parts in the upper portion of the packing box. Compare the delivered parts with the packing list and your actual order. If you find any discrepancies, please contact your local Leica sales organization immediately. Leica TP1020

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3.

Instrument components and specifications

3.3

General overview Leica TP1020 - Type 1 with additional paraffin station (optional accessory)

Carousel cover

Reagent station lid Tissue basket holder Glass container with holder

Standard tissue basket

Paraffin station Container station holder with level indicator

Notch for paraffin station connection cable

Container station platform Control panel

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Instruction manual V 2.4 – 08/2009

3.

Instrument components and specifications

General overview Leica TP1020 - Type 1 with additional paraffin station (optional accessory, continuance)

12 11 10

Sockets for paraffin station connector plugs (socket of station no. 10 closed with a cover cap (not shown))

Voltage selector Mains switch

Paraffin station connector plug

Socket for mains cable

Fig. 3 - TP 1020 - rear side

Leica TP1020

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3.

Instrument components and specifications

3.4

General overview Leica TP1020 - Type 3

Plexiglass fume containment shield

Fig. 4

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Instruction manual V 2.4 – 08/2009

3.

Instrument components and specifications

General overview Leica TP1020 - Type 3 (continuance)

Activated carbon filter receptacle Hose clamp

Fume outlet tube (optional accessory)

Fig. 5

Leica TP1020

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3.

Instrument components and specifications

3.5

Accessories supplied together with the instrument

3.5.1 Standard tissue basket Capacity: 100 cassettes Tissue cassettes are loosely arranged in the basket.

Fig. 6

3.5.2 Glass beaker with holder Capacity: 1.8 l Reagent containers consist of a glass beaker inserted into a metal holder with handle. Two marks in the metal holder serve as minimum and maximum level indicators. When filling the container the level of liquid should not be below the minimum or above the maximum level indication mark.

Fig. 7

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Instruction manual V 2.4 – 08/2009

3.

Instrument components and specifications

3.5.3 Aluminum container with holder (standard outfit in instruments with vacuum function or available as optional accessory for other types) Capacity: 1.8 l Instruments equipped with vacuum function are supplied with aluminum containers instead of glass containers. In aluminum containers the level indications marks are located on the inside of the container. Max Min

When filling the container make sure the filling level is not below the minimum or above the maximum level indication mark.

Fig. 8

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