Instruction Manua
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Instruction Manual For CENTAUR 2 CENTRIFUGE
READ BEFORE USE! Models:
MSB020.CX1.5 MSB020.CX1.1 71100-493-4
Health and Safety at Work MSE UK Ltd is required under the Health and Safety at Work Act, 1974 and other UK legislation as designers, manufacturers, suppliers and importers of articles for use at work to ensure that, as far as is reasonably practicable, articles which we design, produce, supply or import are safe and without risk to health. We are required to provide information on the safety and handling precautions to be observed when installing, operating, maintaining and servicing our products. Such advice is contained in this manual. We are also obliged to update this information should circumstances change and to operate a system to this end. We should also like to point out, however that you as users have an important responsibility in the provision and maintenance of safe working practices and conditions. Accordingly, we draw the following matters to your attention: 1.
This apparatus should only be used as intended and within its design parameters by suitably qualified and trained personnel who have read and understood the relevant sections of this manual.
2.
This manual should be readily available to such personnel at all times.
3.
In addition to that which is written in the manual, normal common-sense safety precautions must be taken at all times to avoid the possibility of accidents. Particular care is required when working with apparatus at high temperature or pressure.
4.
Installation, maintenance, repairs and servicing should only be carried out by a MSE (UK) Ltd approved engineer, and suitably trained personnel should only carry out connection to electrical supplies.
TECHNICAL SUPPORT, WARRANTY SERVICE AND MAINTENANCE UK customers; if you are in any doubt whatsoever regarding the correct use of this apparatus, or if you require any technical data or assistance, please contact the MSE UK Ltd Technical Support Department at: MSE UK Ltd
Worsley Bridge Road Lower Sydenham London SE26 5AZ
Telephone Fax E-mail: Web Page:
+44 (0) 870 609 4097 +44 (0) 208 650 8408 [email protected] www.mseuk.co.uk
OVERSEAS CUSTOMERS: Should contact their local Distributor. ELECTRICITY SUPPLIES: Voltage and frequency MSE (UK) Ltd electrical apparatus is offered and labelled in a number of voltage ranges and, where necessary, different frequencies of mains supply. MSE UK Ltd does not accept any responsibility for the operation of any such apparatus should it be connected to electricity supplies which are normally outside, or vary outside, the stated voltage and frequency values for which it is designed, nor for any consequential loss, damage or injury, howsoever caused.
MSE UK Ltd Warranty Terms and Conditions We hope that you do not have the need to use the extensive warranty cover that MSE UK Ltd. extends to you. However should you have a problem, our prompt response is greatly helped if you have filled in and posted the pre-paid Warranty Registration Card supplied with your new equipment. This entitles you to a one-year warranty from the date of delivery. During this period, component parts proven to be defective in materials or workmanship will be repaired or replaced at our expense. Installation, commissioning and calibration are not covered by this warranty agreement. The MSE approved service agent must be contacted for warranty determination and direction prior to any work being carried out. These warranties are only applicable to new products, and not second hand nor refurbished products even if repaired by MSE UK Ltd. Any such products are covered by separate warranty terms and conditions which will be made available on request. Replacement or repair of component parts or equipment under this warranty shall not extend the warranty to either the equipment or the component part beyond the original one-year warranty period unless agreed in writing by MSE UK Ltd. The above warranties are extended to the original purchaser upon full invoice payment. A purchase receipt or other proof of purchase may be required before warranty service will be performed. These warranties only cover failures due to defective workmanship which occur during the normal operation of the product by the original purchaser, and not failures which result from accident, misuse, abuse, neglect, mishandling, misapplication, alteration, faulty installation, electrical power fluctuations, dust, or other environmental extremes, modification or service other than by an approved service agent or following the written authority of the manufacturer, or damage that is attributable to acts of God. Expendable items such as motor brushes, door seals, lid seals, "O" rings or lamps are excluded. MSE UK Ltd, or its approved service agent, reserves the right to repair defective equipment on the premises of the customer, or at a service station, at the sole discretion of MSE UK Ltd or their approved agent. In the event of return to an approved service centre the customer is responsible for the safe packaging of the instrument and notification to the service centre. Neither MSE UK Ltd nor its agents are responsible for any damage occurring during shipment. Specification and Material Changes: MSE UK Ltd reserves the right to supply our latest and improved models at time of shipment. Taxes: The prices quoted do not include any taxes imposed by the State or Country in which the purchase was made. Installation: Installation of all equipment shall be at the expense of the purchaser unless stated otherwise. Access to the site, and the provision of required utilities e.g. Power, water and drainage to suitable connections, will be the responsibility of the purchaser, and at the purchaser's expense. Limitation of liability: In no event, whether as a result of breach of contract or warranty, shall MSE UK Ltd be liable for any consequential or incidental damages including, but not limited to, loss of profit or revenues, loss of use of the equipment or any associated equipment, down time costs, costs of substitute equipment, costs of labour, costs due to delays or claims of purchaser's own customers for such damages. The purchaser agrees to indemnify MSE Ltd and to hold them harmless from any and all liability, claims, demands, actions, suits, expenses or costs, including attorney's fees relating to such consequential or incidental damages. All expressed and implied warranties, including the warranties of merchantable quality and fitness for a particular purpose, are limited to the application period of one year. Validity: Legal rights vary from country to country and states within countries, so some or all of the exclusions or limitations listed above may not apply, but if any part of these conditions shall be found to be unenforceable it shall not affect the validity or enforceability of the remainder of the conditions.
ELECTRICITY SUPPLY Before connecting this apparatus to the electricity supply, check the information given on the apparatus rating plate and ensure that; A) Your supply is single phase A.C. (alternating current) of the stated frequency with neutral nominally at earth potential. B) Your supply voltage is within the stated range. C) The current rating is within the capacity of your outlet. D) Your plug or electricity supply circuit is fitted with a suitable fuse.
Fuse rating
220v - 240v 5 amp
110v - 120v see note for 110V - 120V
WARNING! This apparatus must be earthed. The wires in the mains lead are coloured in accordance with the following code;
Live Neutral Earth
220v - 240v Brown Blue Green and Yellow
110v - 120v Black White Green
Connect the wires to a non-reversible 3-pin plug as follows; Green and Yellow or Green to terminal marked E (Earth), G (Ground), coloured Green or Green and Yellow or marked with the Earth symbol. Blue or White wires to terminal marked N (Neutral) or Common or coloured Blue. Brown or Black wires to terminal marked L (Live) or Phase or coloured Brown. Note 110v - 120v installations to comply with National and State Wiring Codes. IMPORTANT Consult an electrician if in any doubt or if your supply system has any of the following: No earth. A coloured code different from the above. Reversible plugs. Supply and return leads that are both above earth potential. NOTE: Do not touch plug pins within two seconds of plug removal from socket.
CONTENTS
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1. IMPORTANT INFORMATION... 7 2. GENERAL DESCRIPTION ... 8 2.1 Controls And Indicators ... 9 2.2 Centrifuge Bowl ... 10 2.3 Opening Lid Manually ... 10 2.4 Specifications... 10 3. INSTALLATION ... 12 3.1 Preparation ... 12 3.2 Connecting To 3 Pin Plug ... 12 3.3 Recommendation For Securing Centrifuge ... 13 4. ROTORS AND ACCESSORIES... 14 4.1 Installation Of Rotor ... 14 4.2 Removal Of Rotor ... 14 4.3 Loading The Rotor... 15 4.4 Rotor Speed... 15 4.5 Rotors And Accessories Tables... 16 4.5.1 4.5.2 4.5.3 4.5.4 4.5.5
4 Place Swing out rotor ... 16 4 Place Swing out rotor ... 17 8 Place Swing out rotor ... 18 8 Place Angle rotor ... 19 12 Place Angle rotor ... 20
5. OPERATION ... 21 5.1 Run Procedure ... 21 5.2 Stop Procedure ... 21 5.3 Imbalance ... 21 5.4 Motor Thermal Cut-out ... 21 5.5 Power Failure ... 21 5.6 Lid Safety Circuit ... 22 6. MAINTENANCE ... 23 6.1 Cleanliness ... 23 6.2 Drive Shaft ... 23 6.3 Prevention Of Corrosion ... 23 6.4 O-Rings ... 23 6.5 Sterilisation ... 23 6.6 Disinfecting ... 23 6.7 Inspection Or Renewal Of Motor Brushes ... 23 6.8 Renewing The Fuse... 24 6.9 Removal And Replacement Of Centrifuge Bowl ... 25 6.10 Lid Friction Adjustment ... 25 7. SERVICING... 26 7.1 Calibration Of Speed Control ... 26 7.2 Removal Of The Motor ... 26
CONTENTS 7.3
6
Adjustment Of Balance Detector... 27
8. CIRCUIT DESCRIPTION ... 29 8.1 Power Input ... 29 8.2 Speed Control ... 29 8.3 Tacho Circuit ... 29 8.4 Speed Indication ... 30 8.5 Lid Safety Circuit ... 30 8.6 Balance Circuit... 30 9. ESSENTIAL PARTS LIST ... 32 9.1 Body Assembly ... 32 9.2 Lid and Associated Assembly... 32 9.3 Motor Assembly ... 33 9.4 PCB ‘A’ Assembly... 33 9.5 PCB ‘D’ Assembly... 33 9.6 Instrument Chassis Assembly ... 34 10.
PRODUCT DISPOSAL - ISO 14001 COMPLIANCE... 34
11.
HOW TO OBTAIN SERVICE ON YOUR FALCON CENTRIFUGE ... 35
GENERAL INFORMATION
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1. IMPORTANT INFORMATION The following general instructions, and the precautions regarding the prevention of corrosion in rotors and accessories, as described under MAINTENANCE, must be noted and carefully observed. The maximum load should always be checked before commencing any operation. If the sample has a relative density greater than 1.2, the maximum permissible speed of the rotor should be calculated as given in this publication, and applied. Any liquid spilt in the centrifuge should be removed immediately because corrosion is a major cause of failure in centrifuges. Where particularly corrosive solutions are used, the samples should be placed in sealed containers and all necessary precautions observed. The following list gives some examples of corrosive liquids used, particularly in the biochemical field: Phenol/cresol/water, chloroform/isoamyl alcohol, salt solutions (especially ammonium sulphate), solutions of ammonium hydroxide and solutions of acids such as hydrochloric, trichloroacetic and perchloric acids. It should be noted that many other substances used in different fields are often equally corrosive. Users should always check whether or not a substance being used is corrosive in order to take suitable precautions. NOTE: The following materials are prohibited: Flammable or explosive materials Materials which chemically interact vigorously The load must be distributed symmetrically round the rotating assembly so that it is evenly balanced and complies with the manufacturer's instructions in this respect. After use the sleeves and adaptors in centrifuge rotors should be removed to allow thorough cleaning of rotors and accessories and so prevent corrosion. Special care is necessary when using infective material in centrifuges. Containers, when used with such materials, should be closed while being centrifuged and sterilised immediately after use, using non-corrosive means. NOTE: Sealed containers and related components are intended to be part of biosafety systems such as are specified in international and national biosafety guide lines, and cannot be relied on as the only means of safeguarding workers and the environment when handling pathogenic micro-organisms. Care should be taken to preserve intact, sets of accessories which have been supplied as sets, in which all the members possess closely similar weights. It is advisable to have the centrifuge checked by a competent engineer, preferably the manufacturer's representative, at least every six months. Carbon brushes, whilst being of the highest quality obtainable are expressly excluded from the guarantee. A spare set is included in the accessory pack. The method of replacement is detailed in section 6.7 of this manual.
GENERAL DESCRIPTION
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2. GENERAL DESCRIPTION The Centaur 2 centrifuge is CE marked in line with required European Directives for Electromagnetic and Low Voltage Compatibility, see “Declaration of Conformity” for EC Directive/standard used. 120v units designed for CSA approval to the following standards: CAN/CSA
C22.2 No. 1010.1-92
CAN/CSA
C22.2 No. 1010.2.020-94
INDEPENDENT SPEED SENSING - 120 volt Machines only To comply with certain USA standards the 120 volt machines are fitted with a small window in the cover. This permits independent speed check directly from the rotor with use of an optically coupled tachometer.
GENERAL DESCRIPTION
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2.1 Controls And Indicators
Figure 1. Control Panel of the Centaur 2 Centrifuge 1. The Power On rocker switch, when on (switch illuminated) connects power through to various circuits and components within the centrifuge. 2. The Fuse Holder carries a 20mm long, 5 Amp glass cartridge fuse. It is easily removable. 3. The Timer control allows a run period up to 30 minutes-selected by turning the knob clockwise. When the time selected has run out, the rotor will automatically run down to a stop. If required, the knob may be returned (anti-clockwise) to a lesser time or to the Off position at will. It cannot be turned anti-clockwise between the 'Hold' and '30' 'positions. The Hold position switches the centrifuge for continuous running until manually reset to Off. 4. The Rev/min indicator is a digital display giving continuous read-out of the rotational speed of the rotor, rounded off to the nearest hundred rev/min, e.g. 2500, 3200. 5.
The Speed control allows selection of any speed between 300 rev/min and the maximum allowed for the rotor in use (see section 4.5 Rotors And Accessories Tables) as indicated on the Rev/min digital display.
6. The Imbalance indicator lamp (Red) when on, gives warning that the rotor is misloaded to a marked degree. In this event, drive to the rotor will cease and the rotor will run down to a stop. Loading and balance must be checked and corrected before re-starting the centrifuge. 7. The Lid Unlocked indicator lamp (Amber) when on, indicates that the rotor is at a standstill and the lid may be opened safely. When the rotor is spinning the lamp remains off and the lid is locked.
GENERAL DESCRIPTION
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2.2 Centrifuge Bowl The centrifuge bowl is easily removable for cleaning or disinfection purposes see section 6 (Maintenance). 2.3 Opening Lid Manually An electrical interlock prevents the lid being opened while the rotor is turning, or when the power is disconnected. If access to the rotor is required during a power failure, insert the Lid Lock Release Key (supplied with accessories) to its fullest extent, into the small hole on the top of the lid, near the front; move the point towards the rear against a slight spring pressure to actuate the lid lock mechanism. Press the lid actuator to the right and open the lid. The lid is friction-controlled, to remain open at any intermediate position desired.
Front Figure 2. Manual Over-ride of Lid Lock 2.4 Specifications Dimensions Height
336mm (with lid open - 470mm)
Width
436mm
Depth
487mm
Weight
36kg (without rotor)
Power Supply Input voltage and frequency. Cat. No. MSB020.CX1.5
220/240V
50/60 Hz.
Cat. No. MSB020.CX1.1
110/120V
50/60 Hz.
Operating Humidity
MRH 80% up to 31°C decreasing linearly to 50% RH at 40°C
Altitude
Up to 2000M
NOTE: Motor, as fitted, contains overtemperature cut-out device.
GENERAL DESCRIPTION Accessories Supplied With The Centrifuge Part No. 32600.019 76600.004 88110.024 71100.493 71100.958 71100.1052 82120.143
Description Quantity Fuse 5A (20mm) 1 Lid Lock Release Key 1 Lid Seal Insertion Tool 1 Operating Manual 1 Guarantee Card 240V 1 Guarantee Card 120V 1 Spindle Nut 1 (for Rotors 34121-607, 34117-603, 34117-604 only)
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INSTALLATION
12
3. INSTALLATION IMPORTANT: The rotor must not be fitted when moving or during transit. 3.1 Preparation Remove the centrifuge from its packing and place it on a rigid, level surface. Check that the available power supply corresponds to that stated on the voltage plate fixed to the rear panel of the centrifuge. NOTE: (i) An electrical interlock prevents the lid being opened unless the centrifuge is connected to the power supply. (ii) It is important that, at least, 100mm clearance is allowed at the sides and rear of the centrifuge when placed in the final operating position. NOTE: Laboratory management procedures should require that no person or any hazardous materials are within a 300mm boundary while the centrifuge is operating. 3.2 Connecting To 3 Pin Plug Connect the 3-core cable to a 3-pin plug fitted with a 5 Amp fuse as follows: WARNING! This equipment must be earthed. Brown wire to Live (L) Terminal Blue wire to Neutral (N) Terminal Yellow/Green wire to Earth (E) Terminal Connect the centrifuge to the power supply and press the Power switch on. The Power On and the Lid Unlocked indicators will come on. Push the lid lock actuator to the right and open the lid. NOTE: BS EN 61010-2-020 ‘Particular Requirements for Centrifuges’ states that a remote switch, preferably adjacent to the room exit is a requirement in case of unit malfunction.
INSTALLATION
13
3.3 Recommendation For Securing Centrifuge A Fixing Kit for securing the Centaur 2 centrifuge to a suitably sound worktop is for securing centrifuge available from MSE as an optional accessory. It consists of an anchor bracket which is secured to the bench, or table, by two screws with nuts and washers; and two screws which screw into the body of the centrifuge to secure it to the bracket. See Figure 3.
Figure 3 Securing Centrifuge to work surface using Bench fixing Kit (Part No. 41159.188) If the restraint kit is not used to secure the centrifuge on the bench then, it is the recommendation of BS EN61010-2-020 that a clearance of 300mm is allowed around the base of the centrifuge when in the final operating position. NOTE: Laboratory management procedures should require that no person or any hazardous materials are within a 300mm boundary while the centrifuge is operating.
Figure 3a Recommended Clearances Note: 75mm if solid surface (i.e. wall) otherwise 300mm
ROTORS AND ACCESSORIES
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4. ROTORS AND ACCESSORIES 4.1 Installation Of Rotor Before fitting a rotor to the centrifuge ensure that the drive shaft is lightly smeared with petroleum jelly. Check that the rotor is clean - particularly pockets in angle rotors. Locate the rotor on the drive shaft and, ensuring that the correct spindle nut for the rotor in use is employed (see sections 0 & 4.5), engage and tighten the spindle nut finger tight. 4.2 Removal Of Rotor Press Power switch off. Open the centrifuge lid. Remove all rotor accessories, i.e. buckets, trunnions etc. Loosen the spindle nut a few turns, grip the rotor as illustrated in Figure 4 and pull upwards with the fingers whilst gently rocking the rotor and applying downward pressure with the thumbs, to release it from the drive shaft. Remove the nut and lift the rotor free. Note: Do not attempt to loosen the rotor by tapping on the centrifuge motor spindle.
Figure 4. Removing Rotor from Drive Shaft
ROTORS AND ACCESSORIES
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4.3 Loading The Rotor The rotors must carry a full complement of cups/buckets and/or trunnion rings, but all cups/buckets need not be loaded. Loads must be placed symmetrically about the rotor and opposing loads should balance to within 2 grams. Where possible, balancing by sight is usually sufficient. Before loading centrifuge tubes ensure that the appropriate colour coded-adapter or rubber cushion is correctly positioned in the cup/bucket. Check that all tubes project equally from the upper surface of the rotor. Finally, if using a swing-out rotor, check that all cups of trunnion rings swing freely. If necessary, apply a light smear of petroleum jelly to the rotor pivot slots. Failure to load the rotor correctly can result in serious imbalance and possible damage to the centrifuge. Biologically hazardous material should only be centrifuged in sealed buckets or cups. 4.4 Rotor Speed Maximum Speeds of rotors (See section 4.5) are calculated on the basis of tubes filled with a sample of specific gravity 1.2. If liquids of higher specific gravity are used, the maximum speed of the rotor must be reduced according to the formula:
Where M = New maximum speed N = Maximum rotor speed at 1.2 specific gravity S = Specific gravity of sample used
ROTORS AND ACCESSORIES
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4.5 Rotors And Accessories Tables The centrifuge complies with the requirements of BS4402 only when fitted with one of the following rotors. * Max. rev/min at 240V or 110V Haem. Haematocrit tube
** Supplied as standard with buckets Wass. Wasserman glass tube
# Available as spares
4.5.1 4 Place Swing out rotor 43124-126 (includes spindle nut)
Cup (Set of 4)
Sealed Cup 43551-121 43551-121 43551-121 43551-121 43551-121 43551-121 43551-121 Open Cup 34421-639
Adaptor (Set of 4)
34159-302 (brown) 34159-301 (yellow) 34159-304 (green) 34159-303 (grey) 34159-305 (orange) 34159-306 (blue) As above
Rotor Capacity
Max.* Speed
Max. RCF
Max. Tube Size Dia (mm)
Ht (mm)
4 x 200ml 4 x 100ml
3300 3300
2000 2000
57 39.5
116 113
4 x 50ml
3300
2000
29
113
28 x 15ml
3300
2000
17
110
16 x 10ml DSS
3300
2000
16.5
105
36 x 13∅ mm
3300
2000
13
110
48 x 12 ∅ mm
3300
2000
12
110
As above
3600
2200
ROTORS AND ACCESSORIES
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4.5.2 4 Place Swing out rotor 34121-607
Bucket
Trunnion
34411-912 34134-604 Sealing cap 43561-156 can be used if required 34134-605 34411-909 Sealing cap assy 43561-606 can be used if required
34411-904
34136-102 34136-103
Adaptor/ Cushion
Rotor Capacity
Max.* Speed
Max. RCF
Max. Tube Size Dia (mm) 42 29 17 13
Ht (mm) 125 120 118 118
34142-105** 34144-308 34151-302 34153-302
4 x 100ml 4 x 50ml 8 x 15ml 20 x 7ml
3500 3500 3500 3500
2246 2246 2246 2246
34142-104** 34148-705 34141-114 34141-115 34144-307 34153-304 34151-301
4 x 50ml 4 x 50ml 4 x 1oz 4 x 1oz 4 x 15ml 24 x Haem 8 x Wass
4200 4200 4200 4200 4200 4200 4200
3130 3130 3130 3130 3130 3130 3130
30 118 conical 29 112 Glass Sterilin 17.5 110 7.7 120 12.7 120
34142-101** 34142-101** 43161-103 34148-101 34146-104 34146-103 34146-102
8 x 15ml 16 x 15ml 16 x 15ml 16 x Haem. 16 x 5 or 3ml 16 x 2ml 16 x 0.5 or 1ml
4000 3400 3400 3400 3400 3400 3400
2612 2000 2000 2000 2000 2000 2000
17 17 conical 17 7.7 13 12 9
120 120 115 120 120 100 100
ROTORS AND ACCESSORIES
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4.5.3 8 Place Swing out rotor 43121-111 (includes buckets and spindle nut)
Bucket
34411-905#
Trunnion
Adaptor/ Cushion
34142-101**
Rotor Capacity
8 x 15ml
Max.* Speed
3500
Max. RCF
2200
Max. Tube Size Dia (mm) 17
Ht (mm) 115
ROTORS AND ACCESSORIES
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4.5.4 8 Place Angle rotor 34117-603
Bucket
34411-909 34411-909 34411-909 34411-909 34411-909 34411-909 34411-909
Trunnion
Adaptor/ Cushion
34142-104** 34148-705 34141-114 34141-115 34144-307 34153-304 34151-301
Rotor Capacity
8 x 50ml 8 x 50ml 8 x 1oz 8 x 1oz 8 x 15ml 48 x Haem. 16 x Wass.
Max.* Speed
4600 4600 4600 4600 4600 4600 4600
Max. RCF
3120 3120 3120 3120 3120 3120 3120
Max. Tube Size Dia Ht (mm) (mm) 29 110 conical 29 110 Glass Sterilin 17.5 110 7.7 120 12.7 120
ROTORS AND ACCESSORIES
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4.5.5 12 Place Angle rotor 34117-604
Bucket
34411-904 34411-904 34411-904 34411-904 34411-904 34411-904
Trunnion
Adaptor/ Cushion
34142-101** 43161-103 34148-101 34146-104 34146-103 34146-102
Rotor Capacity
12 x 15ml 12 x 15ml 12 x Haem. 12 x 5 or 3ml 12 x 2ml 12 x 0.5 or 1ml
Max.* Speed
4600 4600 4600 4600 4600 4600
Max. RCF
3000 3000 3000 3000 3000 3000
Max. Tube Size Dia (mm) 17 conical 17 7.7 13 12 9
Ht (mm) 110 110 110 120 100 100