New Brunswick Scientific
New Brunswick Scientific CO2 Incubators
CO-150 Guide to Operations Rev A April 2004
Guide to Operations
40 Pages
Preview
Page 1
Model CO-150 CO2 Incubator MANUAL NO: M1310-0050 Revision A April 17, 2004
NEW BRUNSWICK SCIENTIFIC CO., INC. BOX 4005 • 44 TALMADGE ROAD • EDISON, NJ 08818-4005 Telephone: 1-732-287-1200 • 1-800-631-5417 Fax: 732-287-4222 • Telex: 4753012 NBSCO Internet: http://www.nbsc.com • E-mail: [email protected]
3
INTERNATIONAL OFFICES:
BELGIUM New Brunswick Scientific NV-SA Stationsstraat 180/4 3110 Rotselaar België/Belgique Tel: 32 (0)16 56 28 31 Fax: 32 (0)16 57 27 53 E-mail: [email protected]
GERMANY New Brunswick Scientific GmbH In Der Au 14 D-72622 Nürtingen Deutschland Tel: 49 (0)7022 932490 Fax: 49 (0)7022 32486 E-mail: [email protected]
CHINA New Brunswick Scientific Co., Inc. Room 1501, Xiangjiang Building, No. 18 Lane 1265, Zhongshan Road (W) Shanghai 200051, P.R. China Tel: 86 21 3223 0203 Fax: 86 21 6278 7182 E-mail: [email protected]
THE NETHERLANDS New Brunswick Scientific BV Kerkenbos 1101, 6546 BC Nijmegen P.O Box 6826, 6503 GH Nijmegen Nederland Tel: 31 (0)24 3717 600 Fax: 31 (0)24 3717 640 E-mail: [email protected]
FRANCE New Brunswick Scientific SARL 3, rue des Deux-Boules 75001 Paris France Tel: 33 (0)1 4026 2246 Fax: 33 (0)1 4026 5423 E-mail: [email protected]
UNITED KINGDOM New Brunswick Scientific (UK) Ltd. 17 Alban Park St. Albans, Herts. AL4 0JJ United Kingdom Tel: 44 (0)1727 853855 or 0800 581331 Fax: 44 (0)1727 835666 E-mail: [email protected] Web: www.nbsuk.co.uk
New Brunswick Scientific
User’s Guide
5
CAUTION! This equipment must be operated as described in this manual. If operational guidelines are not followed, equipment damage and personal injury can occur. Please read the entire User’s Guide before attempting to use this unit. Do not use this equipment in a hazardous atmosphere or with hazardous materials for which the equipment was not designed. New Brunswick Scientific Co., Inc. (NBS) is not responsible for any damage to this equipment that may result from the use of an accessory not manufactured by NBS.
New Brunswick Scientific
User’s Guide
7
Copyright Notice New Brunswick Scientific Company, Inc. Box 4005, 44 Talmadge Road Edison, New Jersey 08818-4005 Copyright 2004 New Brunswick Scientific Co., Inc. All Rights Reserved. Reproduction, adaptation, or translation without prior written permission from New Brunswick Scientific is prohibited.
Disclaimer Notice New Brunswick Scientific Co., Inc. reserves the right to change information in this document without notice. Updates to information in this document reflect our commitment to continuing product development and improvement.
Manual Conventions NOTE:
CAUTION!
WARNING!
Bold
New Brunswick Scientific
Notes contain essential information that deserves special attention.
Caution messages appear before procedures which, if caution is not observed, could result in damage to the equipment.
Warning messages alert you to specific procedures or practices which, if not followed correctly, could result in serious personal injury.
Text in bold face type emphasizes key words or phrases.
User’s Guide
9
WARRANTY Every Instrument manufactured by the New Brunswick Scientific Co., Inc. is warranted to be free from defects in material and workmanship. This apparatus, with the exception of glassware, lamps and electrodes (where supplied), is warranted for 1 year against faulty components and assembly and our obligation under this warranty is limited to repairing or replacing the instrument or part thereof, which shall within 1 year following date of shipment prove to be defective after our examination. This warranty does not extend to any NBS products which have been subjected to misuse, neglect, accident or improper installation or application; nor shall it extend to products which have been repaired or altered outside the NBS factory without prior authorization from the New Brunswick Scientific Co., Inc.
New Brunswick Scientific
User’s Guide
11
TABLE OF CONTENTS 1
INTRODUCTION ... 13
2
UNPACKING & INSTALLATION ... 15 2.1 2.2 2.3 2.4 2.5 2.6
3
INSPECTION OF BOXES ... 15 UNPACKING ... 15 UTILITIES... 16 LOCATION ... 16 INSTALLING THE FEET ... 17 SETTING UP ... 17
OPERATION ... 19 3.1 CONTROL PANEL ... 19 3.2 PREPARING FOR OPERATION ... 20 3.3 USING THE HUMIDITY TRAY... 21 3.4 SETTING TEMPERATURE & CO2 ... 21 3.5 REFERENCING CO2 WITH AUTOZERO ... 22 3.6 PROGRAMMING THE ALARM SYSTEM ... 23 3.6.1 Setting High & Low Temperature Alarms... 23 3.6.2 Setting the CO2 High & Low Alarms ... 23 3.6.3 Door Open Alarm ... 24 3.6.4 Alarm Duration ... 24 3.6.5 Alarm Arming Delay... 24 3.7 CHAMBER ALARM SYSTEM ... 25 3.7.1 Temperature Sensor System Alarms ... 27 3.7.2 Over-Temperature Cut-out & Alarm... 27 3.7.3 CO2 Control System Alarm... 28
4
ROUTINE MAINTENANCE... 29 4.1 4.2 4.3
5
GENERAL NOTES ... 29 DAILY CHECKS ... 29 MONTHLY CHECKS ... 29
CLEANING & DISINFECTING ... 31 5.1 5.2
CLEANING ... 31 DISINFECTING ... 31
6
SPECIFICATIONS ... 33
7
OPTIONS & ACCESSORIES... 35 7.1 7.2
8
OPTIONS ... 35 AVAILABLE ACCESSORIES ... 35
DRAWINGS & TABLES... 37 8.1 8.2
LIST OF DRAWINGS ... 37 LIST OF TABLES ... 37
New Brunswick Scientific
User’s Guide
13
1
INTRODUCTION
The model CO-150 CO2 incubator is microprocessor-controlled and designed to ensure accurate and reliable operation. The unit incorporates a simple, door-mounted touchsensitive keypad with two individual three-digit LED displays that allow for easy programming and monitoring of the chamber conditions. A direct heat system, utilizing a thermal heating element, completely surrounds the incubator, providing an even temperature within the chamber. The independently heated outer door is designed to ensure an even distribution of heat, thereby eliminating condensation on the inner door. This system ensures a rapid, controlled return to optimum chamber conditions after a door opening while also preventing any overshoot. A solid-state infrared sensor is used to control the level of CO2, providing excellent reliability and remaining unaffected by humidity. The CO2 system has a semi-automatic zero function (“AutoZero”) that provides a simple process to maintain an accurate level of CO2 within the chamber. The 150-liter chamber and all internal components are manufactured from polished stainless steel. The non-tip shelves, shelf racks and humidity tray are easily removed without tools for thorough cleaning and are capable of being sterilized. Air circulation is achieved without the use of a fan, eliminating ductwork (a potential source of contamination), simplifying cleaning, eliminating vibration and reducing small sample evaporation within the chamber. The outer shell of the incubator is manufactured from zinc-plated stove enamelled steel to give a durable corrosion-resistant finish. The incubator incorporates a two-level alarm system. The chamber-monitoring alarms are programmable and will alert you if temperature or CO2 have not recovered within a preset time after the door has been opened. If it is not required, this system can be disarmed. The system alarms occur only if a problem has developed with system components that require user intervention to rectify. The incubator also incorporates an over-temperature safety system that operates independently from the main control system. The incubator’s direct heat system was designed with optimal use of laboratory space in mind: it allows the most efficient internal volume for the footprint of the instrument. In addition, the incubator’s top panel is specifically designed to support the weight of a second identical incubator stacked directly on top of the first.
New Brunswick Scientific
User’s Guide
15
2 2.1
UNPACKING & INSTALLATION
Inspection of Boxes After you have received your order from New Brunswick Scientific, inspect the boxes carefully for any damage that may have occurred during shipping. Report any damage to the carrier and to your local NBS Sales Order Department or distributor immediately.
2.2
Unpacking
WARNING! At least two people are required for to safely lift your CO-150. Disassemble the wooden shipping crate and remove the protective packing. Save the packing materials for possible future use, and be sure to save this User’s Guide for instruction and reference. To simplify lifting the incubator, leave it on the pallet. You must have at least one person at either side to safely lift the unit. Supported by the base only, remove the incubator by lifting it from the delivery pallet. Locate and remove the parts stored in the Humidity Tray. If any part of your order was damaged during shipping, is missing, or fails to operate, please fill out Customer Satisfaction Form 6300 (packed in the envelope with your warranty card) and return it by fax or mail. You can also call New Brunswick Scientific’s or your distributor’s service department. Using your NBS packing list, verify that you have received the correct materials, and that nothing is missing. Table 1 on the following page outlines the accessory items that are supplied with your new incubator:
New Brunswick Scientific
User’s Guide
16
Table 1: Accessories Provided Quantity 3 2 14 1 1 1 1 3 meters, 9.8 feet 2 4 4 1
2.3
Item Non-tip Shelves Wire Shelf Racks (one for each side) Silicone Rubber Suction Feet Humidity Tray White porous CO2 Sensor Cover Black Sensor Cover Power Cord PVC Tubing, ~1/4-inch or 6mm bore, with an inline CO2 HEPA-filter connected, ready for use Hose Clips Adjustable Feet, with locking nuts Anti-slip Pads for adjustable feet User Manual
Notes Packed Separately Packed Separately Packed in Humidity Tray Packed Separately Installed Installed Packed in Humidity Tray Packed in Humidity Tray Packed in Humidity Tray Packed in Humidity Tray Packed in Humidity Tray Supplied
Utilities In order to use the incubator, you will need: Table 2: Utilities
Utility Electricity
Requirement 110/120V, 50/60 Hz grounded electrical supply with min. capacity of 6 amps OR 220/240V, 50 Hz grounded electrical supply with minimum capacity of 3 amps Cylinder with 100% CO2 vapor withdrawal, together with a two-stage regulator for pressure control to 5 psi or 0.35 BAR
CO2 Gas
2.4
Location The incubator is designed to operate at a chamber temperature of 1.0°C above ambient, and at an absolute minimum ambient temperature of 15°C if the unit is being used at 37°C. Care should be taken to avoid placing the incubator in a position that may affect its performance, such as those listed below. DO NOT place the incubator: • • •
M1310-0050
Directly under, beside or within the air-flow of heating or airconditioning ducts, or other drafts; Directly beside heat generating equipment such as a heater, an autoclave or oven; Near the exhaust of heat- or cold-generating equipment (like a ULT freezer); Model CO-150 CO2 Incubator
User’s Guide
17
•
Near a window exposed to direct, unshaded sunlight.
Remove the unit from the pallet and place the incubator in the working position, on a level surface capable of bearing its weight (approximately 163 lbs./74 kg; actual use weight will be heavier and will depend on the options installed and the material stored in the incubator). The incubator is designed so that one incubator can be safely stacked on top of another identical unit. It is not possible to put any other type of incubator or heavy apparatus on top, as the top cover is designed to support only the feet of another model CO-150.
NOTE: There is no need to remove the top panel for normal maintenance or servicing, so the upper unit does not have to be moved when you are servicing the lower unit. 2.5
Installing the Feet To ensure adequate airflow for correct operation of the relative humidity control system, the incubator feet must be installed. More than one person is required to perform this operation. To insert the adjustable feet: 1. Make sure the locking nuts are installed onto each of the four feet provided. 2. Beginning with the front pair: tilt the incubator toward the back, and screw the feet in, to the required depth. 3. Tilt the incubator forward to install the rear pair of feet. 4. Put an anti-slip pad (provided) on each foot.
2.6
Setting Up Install the power cord: 1. Insert the power cord into its receptacle on the back of the unit (see Figure 3 below). 2. Press the cord firmly into its socket. Install the wire shelf racks and shelves and level the incubator: 1. Each wire shelf rack has silicone suction cups that hold the rack in place. Place one silicone suction cup onto the end of each of the wire rack supports (each marked with an asterisk in Figure 1a below). Be sure to install the suction cup marked R as indicated in this illustration.
New Brunswick Scientific
User’s Guide
18
2. Place the racks inside the chamber, one on each side (they will only fit correctly one way). Two suction cups sit on the bottom of the incubator chamber, and the one marked R (“Rear”) is placed against the back wall of the chamber. Figure 1: Installing Shelf Racks & Shelves R
*
*
*
* *
* * a
HUMIDITY TRAY
b
3. Install the 3 shelves (Figure 1b above). Make sure that each shelf’s anti-tip groove is properly inserted onto each of the wire shelf rack guides. 4. Level the incubator by adjusting the feet. Place a small level on the second shelf of the incubator. Adjust the leveling feet until the incubator is level and stable. Lock the leveling feet in place by tightening the locking nuts on each foot. Install the humidity tray: 1. Install the humidity tray onto the lowest supports of the wire shelf racks (see Figure 1b above). 2. Ensure that the humidity tray is pushed fully back against the rear wall. The tray is designed so that it is always in the optimum position for condensate collection. Connect the CO2 gas supply: 1. Connect the unit to the CO2 supply using the ~1/4-inch or 6mm plastic tubing (with installed HEPA filter) by attaching the tubing from the two-stage regulator (or in-line regulator) to the matching CO2 inlet on the rear of the incubator. 2. Use the tubing clips provided to eliminate CO2 leaks.
M1310-0050
Model CO-150 CO2 Incubator
User’s Guide
19
3 3.1
OPERATION
Control Panel The control panel consists of two individual three-digit LED displays, and four Function keys (see Figure 2 below). Figure 2: Control Panel CO2 DISPLAY (shown in normal operation at factory setpoint)
TEMPERATURE DISPLAY (shown in normal operation at factory setpoint)
FUNCTION KEYS
Table 3: Function Keys Key Symbol
Key Name
∗
Programming
▼
Down
▲
Up
◄
Enter
Function Press this key to enter Programming mode, and to set values in either display. Press this key simultaneously with the Enter key to perform AutoZero. In Programming mode, use this key to scroll down through numbered values in the display. Press this key simultaneously with the Enter key to work in the Alarm system. In Programming mode, use this key to scroll up through numbered values in the display. Press this key to save a new setpoint. Press this key simultaneously with the Programming key to perform AutoZero. Press this key simultaneously with the Down key to work in the Alarm system.
NOTE: If you accidentally press both the Up and Down keys simultaneously, you will engage Engineering Mode: press the Programming key immediately to exit. New Brunswick Scientific
User’s Guide
20
3.2
Preparing for Operation 1. Remove the black protective cover from the CO2 sensor, and store it for use when you clean the incubator. There is a storage holder at the rear of the unit (see Figure 3 below). Figure 3: Rear Panel
FUSE HOLDER
POWER INLET
2. Ensure that the white porous sensor cover is in place. 3. Remove the silica gel dessicant sachet. 4. Using the power cord provided, connect the incubator to a grounded power supply. The display will illuminate immediately.
NOTE: The incubator does not contain a main power switch, and will turn on immediately when connected to a power source. If the power receptacle has a switch, make certain the power receptacle is switched on. When the incubator turns on, the decimal points on the display for Temperature and CO2 flash on and off to signify that the Alarm System is not armed. 5. Turn on the gas supply with the pressure set to 5 psi or 0.35 bar when the incubator is at the programmed temperature.
NOTE: Be certain to check for leaks in the CO2 connections to avoid depleting your CO2 gas supply. This can be accomplished using a solution of soapy water applied to each fitting and checking for bubbles. If any bubbles are noted, readjust the fitting. 6. The chamber setpoints are pre-programmed at 37.0°C and 5.0% CO2. 7. If you are not planning to humidify the incubator, leave the humidity tray in place and skip to Step 4 in Section 3.3 below. M1310-0050
Model CO-150 CO2 Incubator
User’s Guide
21
3.3
Using the Humidity Tray 1. Fill the humidity tray with 1.5 liters of warm (∼ 37.0ºC) distilled water. 2. For cell culture work, we recommend the addition of one small teaspoonful (~0.11oz or 3.2g) of copper sulphate (or another recognized biocide) in the humidity tray. Tests have shown that in addition to inhibiting bacterial growth in the tray, this can reduce contamination on the chamber walls. 3. For IVF and other sensitive work, we do not recommend the use of any biocide in the humidity tray. To reduce the possibility of contamination, every 10 - 14 days the humidity tray water should be changed and the humidity tray should be cleaned with a solution of 70% isopropyl alcohol and 30% distilled water.
NOTE: Ensure that the Humidity Tray is pushed fully back against the rear wall. The humidity tray is designed so that it is always in the optimum position for condensate collection.
CAUTION! Never leave water in the humidity tray if the incubator is switched OFF, as this may cause damage to the CO2 sensor.
4. Let the incubator run for at least two hours (preferably overnight) to allow conditions to stabilize. 3.4
Setting Temperature & CO2 At this time, program the desired Temperature and CO2 setpoints. Temperature and CO2 may be set within the following ranges: Table 4: Temperature & CO2 Setpoint Ranges Parameter Temperature CO2
Available Setpoint Range 10ºC to 50ºC (must be at least 1ºC above ambient) 0.2% to 20%
To set the Temperature: 1. Press the Programming (∗) key.
New Brunswick Scientific
User’s Guide
22
2. Press the Up (▲) or Down (▼) key until the desired value appears in the left display. 3. Press the Enter (◄) key to save the setpoint. 4. Now you can set the CO2 level. To set the CO2 level: 1. Press the programming (∗) key. 2. Press the up (▲) or down (▼) key until the desired value appears in the right display. 3. Press the Enter (◄) key to save the setpoint. If at any time you wish to change the CO2 level without adjusting the Temperature setpoint, press the Programming (∗) key twice to work in the CO2 display.
NOTE: Allow the incubator to stabilize at the setpoints selected before continuing. 3.5
Referencing CO2 with AutoZero The purpose of the AutoZero system is to reference the CO2 sensor to an atmospheric CO2 level of 0.05%. Over a period of time, the sensor baseline may drift, causing an inaccuracy in the CO2 level at the programmed value. When you put your incubator into operation, we recommend that you AutoZero the CO2 system once a month to ensure that the CO2 level is as accurate as possible. After the incubator has been in storage (or transit) for a while, the AutoZero should also be checked, which is why you should run an AutoZero prior to using the incubator for the first time. To perform the AutoZero: 1. If you have not already done so, set the CO2 level (see Section 3.4). There should be an audible “click” when the programmed level is entered: this is the CO2 valve opening.
NOTE: There is a magnetic switch above the outer door, and a magnet mounted in the door that switches off the CO2 valve when the outer door is opened.
M1310-0050
Model CO-150 CO2 Incubator
User’s Guide
23
2. Press the ∗ and ◄ keys simultaneously. 3. Read the instructions that appear in the display; the inner door must be opened and closed according to the displayed instructions, after which the process will be completely automatic. 4. Open the door for 60 seconds to completely degas the chamber. A countdown is shown on the display. 5. When the display instructs you to do so, close the door. An automatic 10-minute countdown will start, to allow the chamber conditions to recover and the CO2 sensor signal to stabilize. 6. At the end of the countdown, the sensor signal will automatically be rereferenced. The incubator will reopen the CO2 valve to allow the CO2 level to recover to the setpoint value.
NOTE: Pressing any key, or opening the door during the AutoZero will automatically terminate the process and control of CO2 will restart. The incubator is now ready for use. 3.6 3.6.1
Programming the Alarm System Setting High & Low Temperature Alarms 1. Press the ◄ & ▼ keys simultaneously to enter Alarm Program Mode. The display will show °C. AL. 2. Press the ◄ key to display the High Temperature Alarm, HI 37.5. The factory setting is the setpoint value (37.0ºC) + 0.5°C. 3. If you wish to adjust the High Temperature Alarm, use the ▲ or ▼ key. The minimum setting, however, is 0.5°C from setpoint. 4. Press the ◄ key to save the setting, whether or not you have changed it. Now the Low Temperature Alarm is displayed, LO 36.5. The factory setting is the setpoint value (37.0ºC) - 0.5°C. 5. Use the ▲ or ▼ key if you wish to adjust the value. 6. Press the ◄ key to accept the setting, whether or not you have changed it.
3.6.2
Setting the CO2 High & Low Alarms 1. While you are still in Alarm Program Mode (see Step 1 of Section 3.6.1 above), press the ▲ key. The display will show CO2 AL.
New Brunswick Scientific
User’s Guide
24
2. Press the ◄ key to display HI.5.5. The factory setting is the setpoint value (5.0%) + 0.5%. 3. If you wish to adjust the High CO2 Alarm, use the ▲ or ▼ key. The minimum setting, however, is 0.5°C from setpoint. 4. Press the ◄ key to save the setting, whether or not you have changed it. Now the Low CO2 Alarm is displayed, LO 4.5. The factory setting is the setpoint value (5.0%) - 0.5%. 5. Use the ▲ or ▼ key if you wish to adjust the value. 6. Press the ◄ key to accept the setting, whether or not you have changed it. 3.6.3
Door Open Alarm When you open the door, an alarm will sound after a preset time delay. If you wish to adjust the time delay: 1. In Alarm Program Mode (see Step 1 of Section 3.6.1 above), press the ▲ key, the display will show doo. r AL (reading across both displays). 2. Press the ◄ key and the ▲ & ▼ keys to adjust the time (as you scroll through the available choices, you will see 15, 30, 45, 60, 75 & 90 seconds, then OFF). 3. Press ◄ to save the desired value.
3.6.4
Alarm Duration The following steps will adjust the length of time during which all audible alarms will last: 1. In Alarm Program Mode (see step 1 of Section 3.6.1 above), press the ▲ key. The display shows Per . Iod (reading across both displays). 2. Press the ◄ key and the ▲ & ▼ keys to adjust the Alarm duration (as you scroll through the available choices, you will see OFF, 10 sec, 30 sec, 60 sec, 600 sec, 1 Hr, then ON). 3. Press ◄ to select the desired value.
3.6.5
Alarm Arming Delay This is the length of time that is allowed for the Temperature and CO2 to recover after opening the incubator before the Alarm System is armed again. This helps prevent unnecessary alarms from occurring. To change the delay: 1. In Alarm Program Mode (see step 1 of Section 3.6.1 above), press the ▲ key. The display will show dr . dEL (across both windows).
M1310-0050
Model CO-150 CO2 Incubator
User’s Guide
25
2. Press the ◄ key and the ▲ & ▼ keys to adjust the Alarm Arming time (as you scroll through the available choices, you will see 0.15 Hr, 0.20, 0.30, 1.00, then OFF). 3. Press ◄ to select the desired value. Press ∗ to return to the main alarm programming menu, and press ∗ again to return to the main display.
NOTE: When alarm delay is set to OFF, the Alarms will arm only when the programmed setpoint is reached. 3.7
Chamber Alarm System When the incubator is switched ON, or after any values have been reprogrammed, the Alarm System is inactive until the setpoint values (± 0.1) are achieved, after which the Alarm System is armed. See Figure 4, Chamber Alarm System Flow Chart, on the following page. If temperature and/or CO2 levels deviate more than the programmed amount, the display flashes, the audible alarm sounds and a message appears on the screen. You can acknowledge (and cancel) the alarm by pressing any key. When the inner door is opened, the Alarm System is disabled. When you close the door, the preset Alarm Arming Delay starts. When the delay time expires, the Alarm System is rearmed; if the temperature and/or CO2 fall below or above the alarm setpoints, the alarm will be activated. If chamber conditions recover within the Alarm Arming Delay time, the Alarm System will be rearmed but no alarm will be activated. If an alarm is not acknowledged but the chamber conditions subsequently recover, the audible alarm will be cancelled but the alarm message will remain on the screen to alert the user to the fact that an alarm has occurred. You can cancel this alarm message by pressing any key. The duration of the audible alarm can be adjusted from inactive to continuous (see Section 3.6.4 above).
New Brunswick Scientific
User’s Guide
26
Figure 4: Chamber Alarm System Flow Chart
CHANGE PROGRAMMED VALUES
SWITCH ON
OPEN INNER DOOR
DISARM ALARMS
CLOSE INNER DOOR ARE TEMPERATURES AND CO2 LEVELS WITHIN +/- 0.1 OF SET POINT? 15 MINUTES ALARM DELAY TIMEOUT STARTED NO
YES
15 MINUTES ALARM DELAY TIMEOUT COMPLETED ARM ALARMS
IS TEMPERATURE OR CO2 LEVEL MORE THAN +/- 0.5 OUTSIDE PROGRAMMED POINTS?
YES
NO
VISIBLE AND AUDIBLE ALARM - MESSAGE ON SCREEN
CANCEL ALARM BY PRESSING ANY KEY
ALARMS CANCELLED
HAVE CONDITIONS RECOVERED TO WITHIN +/- 0.1?
VISUAL AND AUDIBLE ALARMS CANCELLED, MESSAGE LEFT ON SCREEN
CANCEL MESSAGE BY PRESSING ANY KEY
NO
M1310-0050
YES
Model CO-150 CO2 Incubator
User’s Guide
27
3.7.1
Temperature Sensor System Alarms There are four temperature sensors: two in the door and two in the chamber. If any of these sensors should fail, the following message will appear: °C FAIL . Because the incubator can no longer control temperature properly without the failed sensor, the heating will switch off and the incubator will cool down to room temperature. If, however, a sensor fails but subsequently corrects itself, the temperature control will restart and an alarm message will remain on the temperature side of the display: SAL ... (meaning Sensor Alarm). This message can be cancelled by pressing any key.
3.7.2
Over-Temperature Cut-out & Alarm This alarm only occurs if the chamber temperature exceeds the Temperature Setpoint by 1°C or if one of the heating elements exceeds a specific activation threshold. The activation threshold is set at the factory and cannot be adjusted. Following activation, the over-temperature cut-out and alarm system operates in two sequential modes: Mode 1: The over-temperature cut-out and alarm activates and cuts power to the heating elements and the CO2 control valve. The condition is shown on the display by the message ºCt riP . When the chamber temperature has fallen to the programmed setpoint, the system changes to Mode 2. Mode 2: The control system then tries to maintain the chamber temperature at the programmed level by switching the heating elements on and off, using an emergency control method that is, however, less precise. The message tAL. will flash on-screen to signal that an over-temperature fault occurred and the incubator is being controlled by the emergency control system. This message cannot be cancelled via the keypad. Normal temperature control can be regained and the over-temperature cut-out and alarm can be cancelled by reprogramming the temperature, opening and closing the glass door, or switching the incubator off and back on. If the problem persists, the alarm will recur; if this happens, please contact your service representative or your distributor immediately.
New Brunswick Scientific
User’s Guide