RS Biotech Galaxy B User Manual

Section 1 Product Description The Galaxy B CO2 Incubator is designed to ensure accurate and reliable operation. The unit incorporates a touch sensitive keypad and two, three-digit L. E. D. displays which allows easy setting and monitoring of the chamber condition. The microprocessor control system ensures a rapid, controlled return to optimum chamber conditions after a door opening whilst also preventing any overshoot. A thermal heating element completely surrounds the incubator providing an even temperature within the chamber. The independently heated outer door is designed to ensure an even distribution of heat thereby eliminating condensation on the inner door. Incorporated into the unit is an over temperature safety system. This operates independently of the main control system. A purpose designed condensate collection point at the rear of the chamber allows a high uniform relative humidity and prevents undesirable condensation in other parts of the chamber. A solid state infra red sensor is used to control the level of CO2 giving excellent reliability and is unaffected by humidity. The CO2 system has a semi-automatic zero function which provides a simple process for the user to follow to maintain the correct CO2 level within the chamber. There is a two level alarm system. The chamber monitoring alarm is programmable and will alert you if temperature or CO2 have not recovered within a preset time after the door has been opened. If not required, this system can be disarmed. System alarms occur only if a fault has developed which requires user intervention to rectify. Air circulation is achieved without the use of a fan eliminating this potential source of contamination and reducing small sample evaporation within the chamber. The 150 litre chamber and all internal components are manufactured from polished stainless steel. The shelves and shelf racks (which are non-tip) are easily removed for thorough cleansing. The outer shell of the incubator is manufactured from zinc plated stove enamelled steel to give a durable corrosion resistant finish. The outer door and top cover are vacuum formed from ABS plastic and they provide a high level of insulation. There are 4 internal supports below the top cover to allow one unit to be stacked directly on top of another. NB - There is no requirement to remove the top cover for normal maintenance and servicing, therefore the upper unit does not have to be moved when servicing the lower unit. The keypad and display are incorporated into the door permitting maximum usable chamber volume within the overall footprint of the incubator.

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Section 2 Guidance Notes and Routine Checks 2.1 To ensure that chamber conditions remain as stable as possible always minimize the length of time that the inner door is open. The magnetic door catch is specifically designed to make door opening and closing as easy as possible. When opening the inner door, wipe off any small drops of condensate that may have formed on the inner seal. This will avoid a build up of condensation. 2.2 Always use copper sulphate (or a recognised biocide) in the humidity tray. Tests have shown that as well as inhibiting bacterial growth in the tray this also reduces contamination on the chamber walls (one teaspoonful of copper sulphate is recommended). 2.3

Daily

2.3.1

Check that the temperature and CO2 levels are reading within specification.

2.3.2 Check the reserve pressure in the CO2 cylinder (normally 50 bar when full). The design of the incubator ensures very low consumption of CO2 and during normal working conditions a type K cylinder should last approximately 12 months. If there is a significant drop at the cylinder 50 bar pressure, it means that the cylinder is almost empty and should be replaced, ensuring that there are no leaks at any of the joints. 2.4

Monthly

2.4.1

Top up the humidity tray (to a maximum volume of 1.5 litres). The use of warm water (approx. 37.0°C) will ensure a rapid return to optimum chamber conditions. After topping up check that the humidity tray is situated directly beneath the deflector plate on the rear wall of the chamber. This ensures that any condensate collected is returned to the reservoir.

2.4.2

If required, a sample of the gas inside the chamber can be taken using the CO2 sample port and checked using a CO2 gas analyser. The CO2 port is located on the left hand side of the Rear Cover. During sampling please ensure the following:a)

The programmed value for CO2 is lowered by 0.2% to prevent CO2 from being injected into the chamber and giving a false reading.

b)

A flow rate not in excess of 0.5 litres/minute is used to take a sample.

c)

The outer door is kept closed to minimize heat loss through the glass door.

It is recommended that prior to sampling, a CO2 auto zero be performed (see Section 6).

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Section 3 Installation 3.1

Unpack the incubator by removing the cardboard sleeve and the protective packing. Remove the parts in the Humidity Tray and fit the feet as follows:- insert the front pair first, tilt the incubator backwards and screw the feet in to the required depth. Repeat the operation, tilting the incubator forward for the rear pair. To simplify lifting, leave the unit on the pallet. At least one person at either side is required for lifting. In the unlikely event of any transit damage this must be reported within 7 days.

3.2

The following items are supplied with your new incubator:6 Shelf Racks (in situ). 4 x Front / Rear Racks & 2 x Centre Racks 3 shelves (in situ) 1 Humidity Tray (in situ) 1 White Porous CO2 Sensor Cover (in situ) 1 Black Sensor Cover (in situ) There is a holder at the rear of the unit to store the black sensor cover

The following items are supplied in the Humidity Tray:1 Mains power cord 3m of PVC 6mm bore plastic tubing with an in-line CO2 HEPA-vent filter connected ready for use. 3 Hose clips (1 large, 2 small) 4 Adjustable feet 4 Anti-slip pads for the adjustable feet 1 Black Sensor Cover (in situ) There is a holder at the rear of the unit to store the black sensor cover To remove the shelves they are withdrawn until the stops in the Centre Racks are reached, then tilted up to approximately 30°. The shelf can then be removed. The lower shelf position is reserved for the humidity tray, which should always be left in place even if the incubator is not humidified. 3.3

In order to use the incubator you will require:a)

A 220/240 V 50 HZ earthed electrical supply - minimum capacity 3 amps

b)

CO2 gas cylinder (100% CO2 vapour withdrawal) together with a two stage regulator for pressure control to 0.35 BAR. (5lbf / in2).

c)

Copper Sulphate or other biocide for the humidity reservoir (optional).

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3.4 The incubator is designed to operate at a chamber temperature of 1.0°C above ambient and at an absolute minimum ambient temperature of 15°C if being used at 37°C. Therefore care should be taken to avoid placing the incubator in draughts, direct sunlight or near a radiator. 3.5 Place the incubator in the working position. The unit is designed to be sited on a level surface capable of bearing 60kg (approx – actual weight will depend on options fitted). It is recommended that a spirit level be used on the centre of the middle shelf to position the incubator, the feet individually adjusted to compensate for uneven surfaces and the anti-slip pads fitted. The incubator is designed so that one incubator can be safely stacked on top of another identical unit. It is not possible to put any other type of incubator or heavy apparatus on top. The top cover is only supported properly where the feet of another incubator would go. 3.6 Remove the black protective cover from sensor (keep for use when cleaning). Ensure that the white porous sensor cover remains in place. There is a holder at the rear of the unit to store the black sensor cover during incubator cleaning. Remove the silica gel dessicant sachet. 3.7 Connect the incubator to an earthed power supply using the power cord provided. Switch on the incubator at the mains supply. The display will illuminate immediately. 3.8 Connect the CO2 using the 6mm plastic tubing to the CO2 HEPA filter at the rear of the chamber. Use the tubing clips provided to eliminate CO2 leaks. Turn on the gas supply with the pressure set to 0.35 BAR (5lbf/ in2) when the incubator is at the programmed temperature. 3.9

The chamber set points are pre-programmed at 37.0°C and 0.0% CO2

3.10

Leave the incubator until the temperature is at set point. This may take 1 to 2 hours.

3.11 Fill the humidity tray with 1.5 litres of warm distilled water. For cell culture work – we recommend the use of copper sulphate (or recognised biocide) in the humidity tray. Tests have shown that as well as inhibiting bacterial growth in the tray this can reduce contamination on the chamber walls (one small teaspoonful of copper sulphate is recommended). For IVF and other sensitive work – we do not recommend the use of any biocide in the humidity tray. To reduce the possibility of contamination, the humidity tray water should be changed and the humidity tray cleaned with 70% Alcohol / 30% distilled water solution every 10 to 14 days. NB – ensure that the Humidity Tray is pushed fully back against the rear wall.. The Humidity Tray is designed so that it is always in the optimum position for condensate collection. 3.12 Leave the incubator for at least two hours (preferably overnight) to allow conditions to stabilize. 3.13 Perform a CO2 Auto Zero as described in section 6 and program the CO2 level. There should be an audible “click” when the programmed level is entered, this is the CO2 valve opening There is a magnetic switch above the outer door, and a magnet mounted in the door which switches off the CO2 valve when the outer door is open. 3.14

The incubator is now ready for use.

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Section 4 Setting the Humidity Conditions 3.12

If humidification is required, the humidity tray which is located on the lower shelf runners should be pulled forward and filled with 1.5 litres of warm distilled water. For cell culture work – we recommend the use of copper sulphate (or recognised biocide) in the humidity tray. Tests have shown that as well as inhibiting bacterial growth in the tray this can reduce contamination on the chamber walls (one small teaspoonful of copper sulphate is recommended).

3.13

For IVF and other sensitive work – we do not recommend the use of any biocide in the humidity tray. To reduce the possibility of contamination, the humidity tray water should be changed and the humidity tray cleaned with 70% Alcohol / 30% distilled water solution every 10 to 14 days. NB – ensure that the Humidity Tray is pushed fully back against the rear wall. The Humidity Tray is specially designed so that it always directly beneath the deflector plate on the rear wall of the chamber. This ensures that any condensation is returned to the reservoir.

DO NOT LEAVE WATER IN THE HUMIDITY TRAY IF THE INCUBATOR IS SWITCHED OFF, AS THIS MAY RESULT IN DAMAGE TO THE CO2 SENSOR.

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Section 5 Control Panel Description The control panel consists of 2, 3 segment LED displays, and 4 keys. The functions and descriptions of these are described below. bold, italicized text represents messages shown on the display.

∗ ↑ ↓ ‘↵’

PROGRAMMING Key UP Key DOWN Key ACCEPT Key

The decimal points on the display for Temperature and CO2 flash ON / OFF to signify that the Alarm System is not armed.

The picture above shows the screen in normal operation, this is how the screen should be illuminated when running normally.

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Section 6 Programming Temperature, CO2 & Auto Zeroing 6.1 Programming Temperature & CO2 The ‘∗’ Key allows Temperature and CO2 to be programmed within the following ranges: Temperature Range: CO2 Range:

10°C (must be 1°C above ambient) to 50°C. 0.2% to 10%

Once the Temperature has been programmed it must be entered with the ‘↵’ Key, the CO2 can then be programmed. Press the ‘↑’ and ‘↓’ Keys simultaneously to enter the Engineering Mode. If this is inadvertently done, press the ‘∗’ Key to exit.

6.2 CO2 Auto Zero The purpose of the Auto Zero system is to reference the CO2 Sensor to an Atmospheric CO2 level of 0.05%. Over a period of time the sensor zero may drift causing an inaccuracy in the CO2 level at the Progammed Value. It is recommended that the CO2 system is Auto Zeroed monthly to ensure that CO2 level is correct. To carry out the Auto Zero, press the ‘∗’ and ‘↵’ keys simultaneously. The Inner Door must be opened and closed according to the displayed instructions, after which the process is completely automatic. 6.2.1 The Door is opened for 60 seconds to completely degas the chamber. A countdown is shown on the Display after which the instruction to close the door is displayed. 6.2.2 The Door is then closed and a 10 minute countdown starts, to allow the chamber conditions to recover and the CO2 sensor signal to stabilise, after which the sensor signal is automatically rereferenced. 6.2.3 The incubator will now reopen the CO2 Valve to allow the CO2 level to recover to the Programme value. 6.2.4 Pressing any key, or opening the door during the Auto Zero will automatically terminate the process and control of CO2 will restart.

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Section 7 Programming The Alarm System Press the ‘↵’ & ‘↓’ Keys simultaneously to enter Alarm Program Mode. 7.1

Setting the High and Low Temperature Alarms.

The LED display shows °C . AL . . Press the ‘↵’ Key to display the High Temperature Alarm, HI 37.5. The factory setting is the programmed value +0.5°C. This value can be adjusted using the ‘↑’ & ‘↓’ Keys, the minimum setting is 0.5°C from set point. Press the ‘↵’ Key to accept the setting and the Low Temperature Alarm is displayed LO 36.5. Press the ‘↵’ Key to accept the setting. 7.2

Setting the CO2 High and Low Alarms.

Press the ‘↑’ Key, the display will show CO2 . AL . . Press the ‘↵’ Key to display HI . 5.5 Adjustment and the Low Alarm Setpoint are as described in 1) above. 7.3

Door Open Alarm.

To adjust the time delay after opening the door before the alarm sounds, Press the ‘↑’ Key, the display will show doo . rAL Press the ‘↵’ Key and the ‘↑’ & ‘↓’ Keys to adjust the time in the following steps – 15, 30, 45, 60, 75 & 90 seconds, then OFF. Press ‘↵’ to select the preferred value. Press ‘∗’ to return to the Main Alarm Programming Menu. Press ‘∗’ again to return to the main display. 7.4

Alarm Duration.

To adjust the length of time which all Alarms are on for. Press the ‘↑’ Key, the display shows Per . Iod. Press the ‘↵’ Key and the ‘↑’ & ‘↓’ Keys to adjust the Alarm on time in the following steps OFF, 10 sec, 30 sec, 60 sec, 600 sec, 1 Hr , then ON. Press ‘ENT’ to select the preferred value. Press ‘∗’ to return to the Main Alarm Programming Menu. Press ‘∗’ again to return to the main display. 7.5

Alarm Arm Time.

This is the length of time that is allowed for the Temperature and CO2 to recover before the Alarm

System is armed. Press the ‘↑’ Key, the display will show dr . dEL Press the ‘↵’ Key and the ‘↑’ & ‘↓’ Keys to adjust the Alarm Arm time in the following steps – 0.15 Hr (15mins), 0.20, 0.30, 1.00, then OFF. Press ‘↵’ to select the preferred value. Press ‘∗’ to return to the Main Alarm Programming Menu. Press ‘∗’ again to return to the main display. NB – When set to OFF the Alarm will arm only when programmed set point is reached.

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Section 8 The Alarm System Refer to the Flow Chart on the following page. 8.1

At switch on or after the programmed values have been reprogrammed, the Alarm System is inactive until the programmed values ± 0.1 are achieved, after which the Alarm System is armed. If Temperature and / or CO2 levels deviate more than ± 0.5°C / %CO2, the display flashes, the buzzer sounds and a message appears on the screen. This can be cancelled by pressing any key.

8.2

When the inner door is opened the Alarm System is disabled and on closing the door the preset Alarm Arm timeout starts. If chamber conditions recover within the Alarm Arm Time, the Alarm System is rearmed. At the timeout the Alarm System is armed and if the Temperature and CO2 are outside the ± 0.5 alarm point, the alarm will be activated.

8.3

If the alarm is not acknowledged and chamber conditions subsequently recover, the Audio alarm is cancelled but the alarm message is left on the screen to alert the user to the fact that an alarm has occurred. The message can be cancelled by pressing any key.

8.4

The duration of the Audio Alarm can be adjusted from inactive to continuous. See Programming the Alarm System, Section 7.

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Chamber Alarm System Flow Chart

OPEN INNER DOOR

CHANGE PROGRAMMED VALUES

SWITCH ON

DISARM ALARMS

CLOSE INNER DOOR ARE TEMPERATURES AND CO2 LEVELS WITHIN +/- 0.1 OF SET POINT? 15 MINUTES ALARM DELAY TIMEOUT STARTED NO

YES 15 MINUTES ALARM DELAY TIMEOUT COMPLETED ARM ALARMS

IS TEMPERATURE OR CO2 LEVEL MORE THAN +/- 0.5 OUTSIDE PROGRAMMED POINTS?

YES

NO

VISIBLE AND AUDIBLE ALARM - MESSAGE ON SCREEN

CANCEL ALARM BY PRESSING ANY KEY

ALARMS CANCELLED

HAVE CONDITIONS RECOVERED TO WITHIN +/- 0.1?

VISUAL AND AUDIBLE ALARMS CANCELLED, MESSAGE LEFT ON SCREEN

CANCEL MESSAGE BY PRESSING ANY KEY

NO YES

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Section 9 System Alarms 9.1

Temperature Sensor Alarms.

9.1.1

In the event of any one of the four temperature sensor failing (there are two in the door and two for the chamber), the following message will appear °C FAIL

Because the incubator can no longer control temperature properly the heating will switch off and the incubator will cool down to room temperature. If a sensor fails but subsequently corrects itself, the temperature control will restart and an alarm message as follows will be remain on the temperature side of the display SAL ... This message can be cancelled by pressing any key. 9.2 9.2.1

Over Temperature Cut-out / Alarm. This alarm only occurs if the Chamber temperature exceeds the programmed temperature by 1°C or if the element exceeds Set Point + Chamber Control Point Hi +1°C. The activation threshold is fixed and cannot be adjusted.

9.2.2

Following activation, the over temperature cut-out / alarm system operates in 2 sequential modes:Mode 1:- Over temperature cut-out / alarm activates and cuts power to the heating elements and the CO2 control valve. The condition is shown on the display by the message °Ct riP . When the chamber temperature has fallen to the programmed set point, the system changes to mode 2. Mode 2:- The control system then tries to maintain the chamber temperature at the programmed level by switching the heating elements on and off using an emergency less precise control method. The message tAL. will flash on screen to signal that an over temperature fault occurred and the incubator is being controlled by the emergency control system. This message cannot be cancelled from the keypad. 9.2.3 Normal temperature control can be regained and the over temperature cut out / alarm cancelled by reprogramming the temperature, opening / closing the glass door, or switching the incubator off / on. If the problem persists the alarm will re occur, if this happens please consult your distributor. 9.3

CO2 Alarms.

9.3.1 This alarm only occurs if the CO2 Auto Zero cannot adjust to reference the signal to atmosphere at the end of the Auto Zero the following message will appear AUTO ZERO FAI LED This means that the CO2 Sensor is defective and requires replacement. If any of these alarms occur then contact your distributor immediately.

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Section 10. Cleaning and Disinfecting Cleaning 10.1

The exterior of the incubator should be kept clean by wiping over with a soft cloth and soapy water.

Disinfecting 10.2

The recommended disinfecting agent for use with the incubator is a solution of 70% Isopropanol (alcohol) and 30% distilled water. Appropriate safety regulations should be followed when using this solution. Gloves should be worn as a routine precaution. Alcohol can form an explosive vapour in confined spaces – ensure adequate ventilation during cleaning. There will be no adverse effects to the incubator if the following procedures are carried out:-

10.3

Program 0.0% CO2 and switch off the incubator. Disconnect from the mains supply.

10.4

Dampen a clean cloth with the 70 / 30% solution and wipe down all external surfaces. Take care not to let any of the liquid come into contact with any electrical outlets or assemblies.

10.5

Remove all shelves, the humidity reservoir, shelf runners and shelf racks.

10.6

Place the black protective cover over the CO2 sensor, also protect any additional sensors such as Oxygen or humidity with the cover(s) supplied.

10.7

NOTE: It is very important to ensure that no disinfectant solution is spilled onto the white porous CO2 sensor cover at the rear of the chamber. Failure to use the protective cover(s) could result in damage to the sensor(s) and may affect your warranty.

10.8

The humidity reservoir can be cleaned by rinsing in sterile water, wiping down with the alcohol solution, and rinsing with sterile water.

10.9

Wipe down the inside of the chamber with alcohol / water solution, and leave to dry completely.

10.10

Wipe the internal components of the chamber with alcohol / water solution twice. Wipe off excess liquid and leave to dry completely. Re-assemble in reverse order before switching the incubator on. NB:- Check the shelf racks are the correct way round – ie: the small hole in the keyhole slot should be uppermost. Ensure the protective cover(s) are removed from the sensor(s) and replaced in the holder for safe keeping (protective covers must also be removed from any additional sensors fitted such as Oxygen or humidity).

10.11

Refill the humidity tray with 1.5 litres of warm distilled water (approx. 37.0°C) It is recommended that one teaspoonful of copper sulphate or a small quantity of other biocide is added to the water to prevent growth. NB – ensure that the Humidity Tray is pushed fully back against the rear wall.

10.12

Leave the incubator for at least two hours (preferably overnight) to allow conditions to stabilize.

10.13

When the incubator has stabilized, carry out an Auto Zero and re-program the desired CO2 level.

10.14

Never use the following substances to clean the stainless steel as damage will result:

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Sodium Azide, Aqua Regia, Iodine, Ferric Chloride or Sulphuric Acid

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Section 11 Galaxy B Technical Specification Temperature Management Digital programming via microprocessor control on 0.1°C increments. Temperature measurement of the chamber, outer door and both heating elements via 4 resistance-temperature curve matched thermistors with sensitivity <0.1°C “Out of Limits” temperature protection system independent of microprocessor control. Range Control Stability Uniformity

1°C above ambient temperature to 50°C* ± 0.1°C ± 0.1°C ± 0.2°C

Relative Humidity Achieved with stainless steel humidity reservoir Reservoir capacity Relative humidity

1.5 litres 95% at 37°C

Shelves Polished stainless steel – non-perforated. Capacity Standard No

0.2m2 / shelf 3

CO2 Control Solid state infra-red CO2 sensor which operates independent of humidity. Semi-automatic CO2 zeroing facility. Range 0.2 - 10%, or 0.2 - 20% Control 0.1% Stability ± 0.2% Uniformity ± 0.1% Recovery rate Better than 0.7% minute Gas connections 6mm tubing Required gas pressure 0.35 bar / 5psi * Where the incubator is to be run close to ambient temperature it may be necessary to make some adjustments to the control parameters to avoid the temperature overshooting. This can be done via the keypad. Please contact your Distributor for details.

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Dimensions Chamber External Crated

(Height x width x depth) 625 x 485 x 500mm 760 x 575 x 630mm 1000 x 720 x 790mm (Including pallet)

Weight (uncrated) Weight (crated) Chamber volume

60kg (approx – actual weight will depend on options fitted). 80kg 150 litres

Electrical Supply Voltage Power

220 - 240v 50/60Hz 500 watts

Energy required to maintain chamber at 37°C - under 0.1kwh.

Storage Temperature 10 - 50°C

NB:- Factory calibration of the incubator is carried out at 37°C, 5% CO2 & 95% RH in an ambient temperature of 20 - 25°C, with no heat-generating apparatus inside the chamber.

Software calibration adjustments may be required to optimise performance if the incubator is being used well outside of these operating conditions. Performance specifications may also be affected. Please contact RS Biotech, quoting the incubator Serial Number and complete details of operating conditions, for advice on calibration adjustments and relevant performance specifications.

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Section 12 Ordering Information

Galaxy B

Add option numbers to the base number. Galaxy B 150 litre Incubator 0.2% - 10% CO2 220 / 240V 50 / 60 Hz Model 110V 50 / 60 Hz Model

150 - 400 150 – 401

No Charge Options (must be specified when placing order):CO2 Control 0.2 - 20% Left Hand Door

150 - 413 150 - 427

Model Options (factory fitted):Copper interior Seamless Copper interior Seamless Chamber Three Inner Door Six Inner Door Access Port BMS Relay Contact Alarm* Internal IP66 Mains Socket Key Lock on Outer Door

150 - 416 150 - 428 150 - 432 150 - 422 150 - 425 150 - 419 150 – 423 150 - 424 150 – 431

Some option combinations are not possible, others may incur extra cost. Please enquire before ordering. Options marked ‘*’ can be retro-fitted by Service Engineer

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13 Accessories For a full range of accessories, please consult your distributor.

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