This instruction manual is for the Olympus Ergonomic Microscope Model BX45. To ensure the
safety, obtain optimum performance and to familiarize yourself fully with the use of this microscope,
we recommend that you study this manual thoroughly before operating the microscope. Retain this
instruction manual in an easily accessible place near the work desk for future reference.
This publication is printed on 100% recycled paper
Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to assemble
the microscope yourself, please read section 8, “ASSEMBLY” (pages 28 to 30) carefully.
— Be sure to read this section for safe use of the equipment. —
TRANSMITTED LIGHT BRIGHTFIELD OBSERVATION PROCEDURE
USING THE CONTROLS
3-1 Base .................................................................................................................................................................................................................... 8-9
1 Voltage Indication; 2 Using the Light Intensity Preset Switch; 3 Using the Filters
3-2 Focusing Block ...................................................................................................................................................................................... 10
1 Replacing the Fine Adjustment Knob;
2 Adjusting the Coarse Adjustment Knob Tension;
3 Pre-focusing Lever
3-3 Stage ............................................................................................................................................................................................................ 11-12
1 Placing the Specimen; 2 Adjusting the X- and Y-Axis Knob Tension;
3 Rotating the Stage
3-4 Observation Tube ...................................................................................................................................................................... 13-14
1 Adjusting the Interpupillar Distance; 2 Adjusting the Diopter;
3 Using the Eye Shades;
4 Using Eyepiece Micrometer Disks;
5 Adjusting the Tilt
3-5 Condenser .......................................................................................................................................................................................... 15-16
1 Centering the Condenser; 2 Compatibility of Objectives and Condensers
3-6 Immersion Objectives .................................................................................................................................................................. 17
1 Using Immersion Objectives
3-7 Objectives with Correction Collar .............................................................................................................................. 17
3-8 Marker (U-MARKER)........................................................................................................................................................................ 18
4-1 Transmitted Light Phase Contrast Observation .......................................................................... 18-19
4-2 Transmitted Light Darkfield Observation ......................................................................................................... 20
4-3 Transmitted Light Simple Polarization Observation ......................................................................... 21
ASSEMBLY — See this section for the replacement of the light bulb. —
■ PROPER SELECTION OF THE POWER SUPPLY CORD ................................................................... 31-32
This microscope employs a UIS2/UIS (Universal Infinity System) optical design, and should be used only
with UIS2/UIS eyepieces, objectives and condensers for the BX2 series. (Some of the modules designed for
the BX series and objectives/eyepieces for the UIS series are also usable. For details, please consult Olympus
or the catalogues.) Less than optimum performance may result if inappropriate accessories are used.
1. After the equipment has been used in an observation of a specimen that
is accompanied with a potential of infection, clean the parts coming in
contact with the specimen to prevent infection.
· Moving this product is accompanied with the risk of dropping the specimen. Be sure to remove the specimen before moving this product.
· In case the specimen is damaged by erroneous operation, promptly
take the infection prevention measures.
2. Install the microscope on a sturdy, level table or bench so as not to block
the air vents on the underside of the base.
Do not place the microscope on a flexible surface, as this could result in
blocking the air vents and cause overheating or a fire.
3. To prevent obstruction of the natural convection-based air cooling of the
microscope, make sure to leave at least 10 cm of free space between
walls or other objects, and the left, right and rear sides of the microscope
and the lamp socket when installing the microscope.
4. To avoid potential shock hazards and burns when replacing the light
bulb, set the main switch @ to “
” (OFF) then disconnect the power
cord from the wall outlet in advance. Whenever you replace the bulb
during use or right after use, allow the lamp socket ² and bulb to cool
before touching. (Figs 1 & 2)
6V30WHAL (PHILIPS 5761)
# The microscope also incorporate a fuse (this should be replaced by
the manufacturer or authorized agent).
5. Always use the power cord provided by Olympus. If no power cord is
provided, please select the proper power cord by referring to the section
“PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of
this instruction manual. If the proper power cord is not used, product
safety and performance cannot be guaranteed.
6. Always ensure that the grounding terminal of the microscope and that
of the wall outlet are properly connected. If the equipment is not
grounded/earthed, Olympus can no longer warrant the electrical safety
and performance of the equipment.
7. Never insert metallic objects into the air vents of the microscope frame
as this could result in electrical shock, personal injury and equipment
8. The power cord may be melt by the heat of lamp socket if the cord
comes in contact with it. Distribute the power cord at an enough distance from the lamp socket.
The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment in the safest possible manner.
Indicates that the surface becomes hot, and should not be touched with bare hands.
Before use, carefully read the instruction manual. Improper use could result in personal injury to
the user and/or damage to the equipment.
Indicates that the main switch is ON.
Indicates that the main switch is OFF.
Warning engraving/stickers are placed at parts where special precaution is required when handling and using the
microscope. Always heed the warnings.
(Warning against high temperature)
Microscope frame rear panel
(Warning against high temperature)
Should warning stickers become soiled, peeled off, etc., contact Olympus for replacement.
1. A microscope is a precision instrument. Handle it with care and avoid
subjecting it to sudden or severe impact.
2. Do not use the microscope where it is subjected to direct sunlight, high
temperature and humidity, dust or vibrations. (For the operating conditions, refer to section 6, “SPECIFICATIONS”.)
3. When moving the microscope, remove the specimen and modules that
may drop during transport and carefully carry it with the grasping part
on the rear of the arm and the base as shown in Fig. 3 (Weight: approx.
# Damage to the microscope will occur if you grasp it by the stage,
coarse/fine adjustment knob or binocular section of the observation tube.
4. The BX45 can be used with only one intermediate attachment.
Maintenance and Storage
1. To clean the lenses and other glass components, simply blow dirty away using a commercially available blower and wipe
gently using a piece of cleaning paper (or clean gauze).
If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available absolute
Since the absolute alcohol is highly flammable, it must be handled carefully.
Be sure to keep it away from open flames or potential sources of electrical sparks –– for example, electrical
equipment that is being switched on or off.
Also remember to always use it only in a well-ventilated room.
2. Do not attempt to use organic solvents to clean the microscope components other than the glass components. To clean
them, use a lint-free, soft cloth slightly moistened with a diluted neutral detergent.
3. Do not disassemble any part of the microscope as this could result in malfunction or reduced performance.
4. When not using the microscope, keep it covered with a dust cover.
5. When disposing of the microscope. Check the regulations and rules of your local government and be sure to
If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition,
the equipment may also be damaged. Always use the equipment as outlined in this instruction manual.
The following symbols are used to set off text in this instruction manual.
: Indicates that failure to follow the instructions in the warning could result in bodily harm to the
user and/or damage to equipment (including objects in the vicinity of the equipment).
# : Indicates that failure to follow the instructions could result in damage to equipment.
} : Indicates commentary (for ease of operation and maintenance).
This device complies with the requirements of directive 98/79/EC concerning in vitro diagnostic
medical devices. CE marking means the conformity to the directive.
NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device,
pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection
against harmful interference when the equipment is operated in a commercial environment. This
equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in
accordance with the instruction manual, may cause harmful interference to radio communications.
Operation of this equipment in a residential area is likely to cause harmful interference in which case
the user will be required to correct the interference at his own expense.
FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance
could void the user’s authority to operate the equipment.
}If you have not yet assembled the microscope, read section 8, “ASSEMBLY” (pages 28 to 30).
Interpupillary distance adjustment scale
Diopter adjustment ring
Condenser centering screws
Slider insertion slot
Main switch (Page 1)
Slide holder (Page 11)
Voltage indicator LED
Light intensity preset
switch (Page 8)
Aperture iris diaphragm ring
Light intensity preset
Turret (Page 5)
Y-axis knob (Page 12)
X-axis knob (Page 12)
Brightness adjustment knob (Page 8)
(Lamp voltage adjustment knob)
Field iris diaphragm ring (Page 15)
Coarse adjustment tension adjustment ring (Page 10)
Filter slider (Page 9)
Coarse adjustment knob (Page 10)
Top lens swing-out lever (Page 15)
Fine adjustment knob (Page 10)
Fine adjustment knob rubber cap
Pre-focusing lever (Page 10)
Condenser height adjustment knob (Page 15)
View when disassembled from the microscope frame.
Aperture iris diaphragm (Page 16)
Phase contrast/darkfield ring mount (Pages 18 - 20)
Optical devices for phase contrast
Optical devices for darkfield
Filter mount (Bottom side) (Page 9)
Mountable filters (32 mm diameter)
· 32C (provided), (32LBD)*
* Refer to Page 9 for precautions
TRANSMITTED LIGHT BRIGHTFIELD OBSERVATION
}As the phase contrast, darkfield and simplified polarized light observations using transmitted light requires preparation using
optical devices such as an analyzer or polarizer, they will additionally be detailed in section 4, “OBSERVATION”.
Set the main switch to “ I ” (ON) and adjust the
²Brightness adjustment knob
Select the BF (brightfield) light path by turning
Select the light path (trinocular tube).
|Light path selector knob
‡Top lens swing-out lever
ŠCoarse/fine adjustment knobs
‹Diopter adjustment ring
ŒCondenser height adjustment
™Condenser centering screws
šAperture iris diaphragm ring
›Field iris diaphragm ring
‡Top lens swing-out lever
Insert the required filters.
Adjust the brightness.
²Brightness adjustment knob
Place the specimen on the stage.
Engage the 10X objective in the light path.
Then engage the top lens in the light path.
Bring the specimen in focus.
Adjust the interpupillary distance.
Adjust the diopter.
Adjust the light axis.
Adjust the aperture iris and field iris diaphragms.
Engage the desired objective in the light path
and bring the specimen in focus.
| (Trinocular observation tube only)
} Copy the observation procedure pages on separate sheets and post it near your microscope.
USING THE CONTROLS
1. Turn the brightness adjustment knob @ clockwise to increase the voltage and make illumination brighter.
2. The numerals ² around the knob indicate the approximate voltage.
Using the Light Intensity Preset Switch
}The light intensity preset switch @ makes it possible to limit the light
intensity to a preselected level regardless of the position of the brightness adjustment knob. The light intensity preset switch has been set to
about 4 V at the factory.
1. Press the light intensity preset switch @ to the ON position. (The face of
the switch lights when it is ON.)
2. Using a small flat-blade screwdriver, turn the preset adjustment screw ²
to obtain the required light intensity. Turning the screw clockwise increases
3. When the light intensity preset switch is set to OFF, the brightness returns
to the level set by the brightness adjustment knob.
}While the light intensity preset switch is ON, turning the brightness adjustment knob does not affect brightness.
Using the Filters
(Figs. 6 - 8)
}You can place a filter in the light path with either method. (Only the 32C
daylight filter is provided with the microscope.)
· Place a 32 mm diameter filter on the filter slider and engage it in the light
path. (Page 9)
· Insert up to three 32 mm diameter filters on the bottom side of the turret
and turn to engage the filters in the light path. (Page 9)
Mounting a Single Filter
One of the filters listed below can be engaged in the light path by inserting the filter @ in the filter slider ² and engaging the filter slider in the
For light brightness control, transmittance 6%
For light brightness control, transmittance 25%
32C (provided), 32LBD
For daylight/color balancing
For B&W contrast (Green)
Using the Turret (Fig. 7)
}The top and bottom parts of the turret are integrated. Therefore, the filters
to be mounted in the bottom part are determined by the aperture iris
diaphragm positioning and optical devices inserted in the upper part.
: 32C, ND, (32LBD)
1. Loosen the turret clamping screw ³ using the Allen screwdriver, and pull
out the turret |.
2. Place the turret upside down and remove the filter clamping ring ƒ by
pushing its knob section.
3. Place the required filters and set the clamping ring ƒ to the original
position by engaging it with three hooks …. (Fig. 8)
}When an interference filter (32LBD or 32IF550) is used, flare or ghost may
be observed. The flare or ghost may be reduced by inserting the interference filter in the filter slider and placing the ND filter in the turret.
(Examples) · Aperture iris
· Phase contrast (PH)
· Darkfield (DFA)
3-2 Focusing Block
# The stage of this microscope is set at a low position. Take care not to let your hand interfere with the stage when
operating the coarse adjustment knob.
Adjusting the Coarse Adjustment Knob
# Adjust the coarse adjustment knob tension using the tension adjustment ring.
The coarse adjustment knob tension is preadjusted for easy use. However, if desired, you can change the tension using the tension adjustment ring @. Turning the ring in the direction of the arrow increases
tension, and vice versa.
The tension is too low if the objective drops by itself or focus is quickly
lost after adjustment with the fine adjustment knob. In this case, turn the
ring in the direction of the arrow to increase tension.
# The fine adjustment knob is attached to the right side when the
microscope is shipped from the factory.
}The fine adjustment knob is designed detachable to prevent interference
with hand during manipulation of the X- and Y-axis knobs.
Usually attach the knob on the opposite side to the X- and Y-axis knobs.
1. Pull and remove the rubber cap @ from the fine adjustment knob.
2. Using the Allen screwdriver, loosen the clamping screw ² and remove
the fine adjustment knob ³.
3. Remove the seal from the fine adjustment knob screw hole on the other
side and attach the knob by reversing the removal procedure.
4. Attach a provided seal on the screw hole ƒ of the fine adjustment dial |,
from which the fine adjustment knob has been removed.
}The fine adjustment dial | can be operated with your fingertip or finger
surface at the same time as manipulating the X- and Y-axis knobs.
Replacing the Fine Adjustment Knob
}The pre-focusing lever ensures that the objective does not come in contact with the specimen and simplifies focusing.
After focusing on the specimen with the coarse adjustment knob, turn
this lever @ in the direction of the arrow and lock; the lower limit on
coarse adjustment movement is set at the locked position.
After changing a specimen, refocusing is easily accomplished by rotating the coarse adjustment knob to reach the pre-focused position, then
making fine adjustments with the fine adjustment knob.
}The objective’s vertical movement activated by the fine adjustment knob
is not locked.
Placing the Specimen
# The dimensions of the slide glass should be 26 x 76 mm with thickness of 0.9 to 1.4 mm, and the cover glass should have thickness of
# When observing very large specimens, remove the slide holder and
place the specimen directly on the stage.
Microscopy with Double-Slide Holder (Fig. 12)
1. Turn the coarse adjustment knob @ to raise the objective.
2. Open the spring-loaded curved finger ² on the slide holder and place
one or two specimen slides on the stage from the front.
3. After placing the sides as far as they will go, gently release the curved
Microscopy with Single-Slide Holder (Fig. 13)
The specimen side can easily be placed by sliding it into the slide holder
from the front.
Using an Oil Immersion Objective
Adsorption of immersion oil can cause the specimen to drift. In such
cases, it is recommended to use the optional BH2-SCB-3 specimen clip
³ for oil immersion objectives. (Fig. 14)
Adjusting the X- and Y-Axis Knob Tension
1. Hold the X-axis knob @ and slide up the Y-axis knob ² up to expose the
2. Turning the X-axis adjustment knob ³ or Y-axis adjustment knob | clockwise (in the direction of the arrow) increases the tension and counterclockwise decreases it.
# If the tension is adjusted to tight, a creaking sound may be heard
during stage travel, and the stage stopping accuracy may be imperiled.
After long hours of use, the stage guide may be deviated and the stage travel range may be decreased.
However, this is not malfunction and can be corrected
easily as described below.
Horizontal direction: Hold the specimen holder and move the stage guide
to the left and right so that it hits the stoppers.
Vertical direction: Hold the upper stage and move it to the front and rear
so that it hits the stoppers.
Rubber Caps for X- and Y-Axis Knobs (Optional)
}When the X- and Y-axis knobs are fitted with the rubber caps, the knobs
can be adjusted without slipping and fine adjustment is possible by
holding the knobs with a very light force. The rubber caps also reduce
fatigue after long hours of operation.
The U-SHGT thick type (thickness 5 mm) and U-SHG thin type (thickness
2 mm) rubber caps are available.
To attach the rubber caps:
First fit the larger knob rubber to the Y-axis (upper) knob from below it,
then fit the smaller knob rubber to the X-axis (lower) knob from below it.
Rotating the Stage
1. Using the Allen screwdriver, slightly loosen the stage clamping screw @.
2. The stage can be rotated both clockwise and counterclockwise.
# A click may be heard and felt during rotation. However, this is due to
the construction of the substage and does not indicate a malfunction.
}The angle of rotation varies depending on the X- and Y-axis knobs.
Angle of Rotation
Right hand knobs
Left hand knobs
3-4 Observation Tube
Adjusting the Interpupillar Distance
While looking through the eyepieces, adjust for binocular vision until the
left and right fields of view coincide completely. The index dot · indicates
the interpupillary distance.
}Note your interpupillary distance so that it can be quickly duplicated.
Adjusting the Diopter
1. Looking through the eyepiece without the diopter adjustment ring, rotate
the coarse and fine adjustment knobs to bring the specimen into focus.
2. Looking through the eyepiece with the diopter adjustment ring, turn only
the diopter adjustment ring @ to focus on the specimen.
Using the Eye Shades
When Wearing Eyeglasses
Use with the eye shades in the normal, folded-down position. This will
prevent the eyeglasses from being scratched.
When Not Wearing Eyeglasses
Extend the folded eye shades in the direction of the arrow to prevent
extraneous light from entering between the eyepieces and eyes.
When the WHN10X-H (or WHN10X) eyepieces are used, an eyepiece
micrometer disk can be inserted in one of them. When the eyepiece
does not have a diopter adjustment mechanism, however, it is hard to
focus on the micrometer disk if the operator has poor eyesight. Should
that be the case, adjust the focus with eyeglasses on.
Use an eyepiece micrometer disk with a diameter of 24 mm and
thickness of 1.5 mm.
Following Fig. 11, turn the built-in micrometer-mounting frame ² counterclockwise to remove it from the eyepiece and place a micrometer disk.
Using Eyepiece Micrometer Disks
Adjusting the Tilt (with the U-TBI3)
}Adjust the height and tilt of the observation tube to obtain the most comfortable viewing position.
Holding the binocular section with both hands, raise or lower it to the
# Never attempt to force the binocular section past the upper or lower
stop position. Applying excessive force could destroy the limiting
# When the U-TBI3 is used, part of the peripheries of the field of view
may become dark as the aperture iris diaphragm is stopped down
to approximately the minimum aperture.
# When the U-TBI3 widefield tilting binocular is used in combination
with a U-EPA2 intermediate attachment, the light in the peripheral
sections of the field may be dark.
With the U-ETBI/U-TTBI (Fig. 22)
The U-ETBI and U-TTBI are ergonomic observation tubes with normal
field, capable of the tilting adjustment as well as the adjustment of the
eyepiece position toward the front and rear (by 45 mm). The U-ETBI is the
erect image model and the U-TTBI is the inverted image model, and
both models are of the same size.
Centering the Condenser
(Figs. 23 - 25)
1. Turn the condenser height adjustment knob @ to raise the condenser to
its upper limit, then use the top lens swing-out lever ² move the top lens
into the light path.
2. Focus on the specimen using the 10X objective.
3. Rotate the field iris diaphragm ring ³ in the direction of the arrow so that
the diaphragm image comes inside the field of view.
4. Manipulate the condenser height adjustment knob @ to focus on the
5. Insert the two condenser centering screws | into the condenser centering thread holes (below the marking) and turn the screws to move the
iris diaphragm image to the center of the field of view.
6. Gradually open the field iris diaphragm. The condenser is properly centered if the iris image is centered and inscribed in the field of view.
7. During actual use, open the field diaphragm slightly until its image circumscribes the field of view.
}After completing the condenser centration, store the centering screws in
the accommodation positions on the right side of the microscope frame
(page 4) so as not to lose them.
Effects of Field Iris Diaphragm (Fig. 25)
The field iris diaphragm restricts the diameter of the beam of light entering the objective and thus excludes extraneous light, improving image
contrast. The diameter of the field iris should e adjusted for objective
power to the extent that it just circumscribes the field of view. (See “Compatibility of Objectives and Condensers” on the next page.)
}With the 100X objective, the field iris diaphragm image cannot be observed unless the iris diaphragm is minimized. With the 4X objective,
maximize the iris diaphragm to observe it.
Aperture Iris Diaphragm (Figs. 26 & 27)
· The aperture iris diaphragm determines the numerical aperture of the
illumination system. Matching the numerical aperture of the illumination
system with that of the objective provides better image resolution and
contrast, and also increases the depth of focus.
· Since the contrast of microscope specimens is ordinarily low, setting the
condenser aperture iris diaphragm to between 70% and 80% of the N.A.
of the objective in use is usually recommended. If necessary, adjust the
ratio by removing the eyepiece and looking into the eyepiece sleeve
while adjusting the aperture iris diaphragm ring @ until the image shown
in Fig. 26 is seen.
}Using the numerical aperture scale:
Set the condenser numerical aperture scale to about 80% of the NA
value ² of the respective objective. (Fig. 27)
Example: With the UPlanFLN40X (NA 0.75), set the scale to 0.75 x 0.8 = 0.6.
Compatibility of Objectives and Condensers
1.25X - 4X*
Applicable by swing the top lens out.
Applicable by engaging the top lens in the light path.
* When using a 4X or lower-power objective, fully open the condenser aperture iris diaphragm and use the field iris
diaphragm in the base as aperture diaphragm. With the 1.25X to 2X objectives, the peripheral sections of the field of view
may be dark but observation is still possible.
}If you want objectives for cytological observation, please purchase the PlanN10XCY and PlanN series objectives or the
PlanFLN10XCY and UPlanFLN series objectives.
3-6 Immersion Objectives
# Be sure to use the provided Olympus Immersion oil.
Using Immersion Objectives
1. Focus on the specimen with objectives in the order of lower-power to
2. Before engaging the immersion objective, place a drop of provided
immersion oil onto the specimen at the area to be observed.
3. Turn the revolving nosepiece to engage the immersion objective, then
focus using the fine adjustment knob.
# Since air bubbles in the oil will affect the image quality, make sure
that the oil is free of bubbles.
a. To check for bubbles, remove the eyepiece and fully open the field and
aperture iris diaphragms, then look at the exit pupil of the objective inside
the observation tube. (The pupil should appear round and bright.)
b. To remove bubbles, turn the revolving nosepiece to repeatedly defocus
and refocus the oil immersion objective.
# With this condenser, do not use immersion oil in the space between
the top lens and specimen.
4. After use, remove immersion oil from the objective front lens by wiping
with gauze slightly moistened with absolute alcohol.
Caution in use of immersion oil
If immersion oil enters your eyes or contacts with your skin, immediately
take the following treatment.
Eyes: Rinse with fresh water (for 15 minutes or more).
Skin: Rinse with water and soap.
If the appearance of the eyes or skin is altered or pain persists, immediately see your doctor.
3-7 Objectives with Correction Collar
}If the cover glass thickness is not 0.17 mm, the objectives cannot manifest their performances. If a correction collar equipped objective is used
in this case, the difference in thickness can be compensated for by adjusting the collar.
· If the cover glass thickness is known, set the correction collar @ to that
value. (Fig. 29)
· If the cover glass thickness is unknown, adjust the correction collar @
and fine adjustment knob alternately until the positioning with the highest resolution is obtained.
# Be careful not to touch the correction collar @ when turning the
3-8 Marker (U-MARKER)
}Mounting the marker @ on a 10X objective allows you to mark the desired positions with a simple, one-touch operation.
Marking can be controlled by either the left or right hand, depending on
how the marker is installed.
(For details, refer to the Instruction Manual.)
}If you want objectives for cytological observation, please purchase the
PlanN10XCY and PlanN series objectives or the PlanFLN10XCY and
UPlanFLN series objectives.
}When 10X and 40X objectives are switched alternately for observation,
brightness adjustment after switching the objectives becomes unnecessary if the PlanN10XCY or PlanFLN10XCY (ND filter built-in type) is used
because the same observation brightness is achieved at 10X and 40X.
However, with high-contrast specimens, ghosting is likely to occur due to
the characteristeics of the ND filter.
# To prevent the pen tip of the marker from drying up, always attach
the exclusive cap ² on the pen tip whenever it is not used.
}This chapter describes the observation methods other than the transmitted light brightfield observation.
4-1 Transmitted Light Phase Contrast Observation
(Figs. 31 & 32)
1. Loosen the turret clamping screw @ with the Allen screwdriver, and pull
out the turret ².
2. Insert the two centering screws ³ (common use with the condenser
centration) into the optical device centering thread holes and turn the
centering screws counterclockwise.
3. Insert the optical device into the mount hole by applying pressure onto
the plate spring ƒ with the PH ring |.
4. Turn the centering knobs ³ clockwise for an optimum tightness for stabilizing the optical device.
5. Attach the seal provided with the optical device to the sealing position ….
6. If you want to insert a PH ring in another hole, perform steps 2 to 5 above.
7. Remove the centering knobs ³ and place the turret in the original position.
(Insert the turret in the direction of the arrow on the dovetail.)
Mounting the Optical Device (PH Ring)
(Figs. 33 - 35)
# Disengage the analyzer and polarizer from the light path, and replace the objective with a Ph objective.
1. Engage the Ph objective in the light path.
2. Turn the turret @ to engage the suitable PH ring for the Ph objective in
the light path.
Applicable Ph Objectives
PlanCN10XPh, PlanCN20X, PlanN10XPh, PlanN20XPh,
PlanCN40XPh, PlanN40XPh, UPlanFLN40XPh
PlanC100XOPh, PlanN100XOPh, UPlanFLN60XOPh,
Applicable Ph Objectives
Ach10-XPh, Ach20XPh, Plan10XPh, Plan20XPh,
UPlanFl10XPh, UPlanFl20XPh, UPlanApo10XPh
Ach40XPh, Plan40XPh, UPlanFl40XPh, UPlanApo20XPh
Ach100XOPh, Plan100XOPh, UPlanFl100XOPh,
UPlanApo40XOPh, UPlanApo100XOIPh, PlanApo60XOPh
3. Adjust the field iris diaphragm ² so that circumscribes the field of view.
4. Place the specimen on the stage and focus on the specimen.
5. Remove one of the eyepieces and replace it with the U-CT30 centering
6. Turn the upper part of the U-CT30 centering telescope to focus on both
the bright ring (ring slit) and darker ring (phaseline of the objective).
7. Insert the two centering screws ³ into the optical device centering thread
holes (above the markings) and adjust centering of the PH ring so that
the bright ring overlaps the dark ring in the field. (Fig. 34)
# If more than one PH ring slit image is displayed, center the brightest
PH ring slit image.
# Be sure to remove the centering knobs when it is required to turn
8. Repeat steps 6 and 7 above for each objective with different power.
9. Remove the U-CT30 centering telescope and replace it with the eyepieces.
Store the centering screws in the accommodation positions | on the
right side of microscope frame so as not to lose them. (Fig. 33)
4-2 Transmitted Light Darkfield Observation
Mounting the Optical Device (DF Ring)
}The optical device (DF ring) can be mounted in the same way as the optical device (PH ring). Please see page 18.
# Disengage the polarizer and analyzer from the light path.
1. Turn the turret (@ in Fig. 33) to engage the BX45-DFA in the light path.
2. Engage an applicable objective in the light path.
PlanCN10X, PlanCN20X, PlanCN40X, PlanN10X, PlanN20X, PlanN40X, PlanN50XOI,
UPlanFLN10X, UPlanFLN20X, UPlanFLN60XOI, UPlanFLN100XOI, UPlanSApo10X
Ach10X, Ach20X, Ach40X, Plan10X, Plan20X, Plan40X, Plan50XOI, UPlanFl10X, UPlanFI20X,
UPlanFI100XOI3, UPlanApo10X, UPlanApo20X, UPlanApo100XOI3
* Objectives with magnification of 10X or more and NA or 0.7 or less can be used.
Objectives equipped with iris diaphragm can also be used if their NA can be reduced to no more than 0.7.
3. Place the specimen on the stage and focus on the specimen.
4. Remove the eyepieces, look into the eyepiece sleeves to locate the objective pupil, and turn the optical device centering
screw holes (above the markings) to center the DF ring so that no light exits through the objective pupil.
5. Attach the eyepiece again, observe the darkfield image, and repeat centering to obtain the best possible darkfield effects.
6. Adjust the condenser height to obtain uniform darkfield illumination across the upper and lower halves of the condenser
7. Open the field iris so that regular illumination is obtained.
When switching the darkfield observation objective or the observation mode between darkfield and another
method, be sure to keep your eyes away from the eyepiece.
When you switch the objective or turret between the darkfield and other positions while looking into the eyepiece.
Otherwise, the direct light may enter your eyes.
4-3 Transmitted Light Simple Polarization Observation
}Polarized light observation requires the U-ANT analyzer (or any module incorporating an analyzer) and the BX45-PO
Mounting the BX-45PO Polarizer
1. Pull out the filter slider @.
2. Insert the BX45-PO polarizer ² into the hole all the way until stopped.
}The polarizer levers should extend in the lateral direction. This position is
the Cross-Nikol (dark) position.
Mounting the U-ANT Analyzer
1. Remove the rubber cap on the slider inlet on the upper part of revolving
2. Hold the U-ANT analyzer ² so that the side with indications faces up,
then align index markings and drop the analyzer into the dummy slider
@ which is provided with the BX45-PO. (The analyzer will be clamped by
3. Place the dummy slider with the U-ANT analyzer @, push the dummy
slider fully in to engage the analyzer in the light path.
}The analyzer can be disengaged from the light path by pulling the dummy
slider by one step.
1. Turn the turret (@ in Fig. 33) to select BF of the transmitted brightfield
observation light path.
2. Engage the objective in the light path.
# When a Ph objective is used, the contrast may be weakened.
3. Turn the polarizer lever slightly so that the field becomes darkest (Cross
4. Place a specimen and focus on it.
5. Adjust the field iris diaphragm so that it circumscribes the field of view.
6. The contrast may be increased by adjusting the aperture iris diaphragm.
Under certain conditions, performance of the unit may be adversely affected by factors other than defects. If problems occur,
please review the following list and take remedial action as needed. If you cannot solve the problem after checking the
entire list, please contact your local Olympus representative for assistance.
1. Optical System
a) Bulb does not light.
Bulb is burned out.
Power cord is unplugged.
Plug power cord into the power outlet.
b) Bulb operates, but field of view re- Aperture and field iris diaphragms are not Adjust them to proper sizes.
opened wide enough.
c) Field of view is obscured or not Revolving nosepiece is not correctly en- Make sure that the revolving nosepiece
clicks properly into place.
Condenser top lens is not set correctly.
Swing out the top lens.
Condenser is not properly centered.
Center the condenser.
Turret is set in an intermediate position.
Set turret to a click position.
Field iris diaphragm is stopped down too Open the field iris diaphragm until it
circumscribes the field.
Bulb is not mounted correctly.
Push the pins of halogen bulb all the
way until the stop position.
d) Dirt or dust is visible in the field of Dirt/dust on the eyepieces.
Dirt or dust on condenser top lens, turret
upper surface covering glass
Dirt/dust on the specimen.
Visibility is poor.
Image is not poor.
Contrast is poor.
Details are indistinct.
A non-UIS2/UIS objective is used.
Use only UIS2/UIS series objectives
with this microscope.
Aperture iris diaphragm is stopped down Open aperture iris diaphragm.
Correction collar on correction collar While focusing, turn the correction
equipped objective is not properly ad- collar to find the best position.
Front lens of objective is dirty.
Immersion oil is not being used with an Use immersion oil.
oil immersion objective.
Immersion oil contains bubbles.
Remove the bubbles.
Recommended immersion oil is not Use the provided immersion oil.
Dirt/dust on specimen.
Dirt/dust on condenser top lens, turret or
upper surface covering glass.
Inappropriate object side or cover glass Replace with glass of recommended