BX53 SYSTEM MICROSCOPE Instructions Sept 2013

In accordance with European Directive 2002/96/EC on Waste Electrical and Electronic Equipment, this symbol indicates that the product must not be disposed of as unsorted municipal waste, but should be collected separately. Refer to your local Olympus distributor in EU for return and/or collection systems available in your country.

NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment. This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the instruction manual, may cause harmful interference to radio communications. Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense. FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance could void the user's authority to operate the equipment.

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BX53

CONTENTS

Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to assemble the microscope yourself, please read section 10, “ASSEMBLY" (pages 34 to 37) carefully.

IMPORTANT -- Be sure to read this section for safe use of the equipment. --

1-3

1

MODULE NOMENCLATURE

2

CONTROLS

5-7

3

FLOW OF OBSERVATION

8,9

4

SIMPLIFIED OBSERVATION PROCEDURE

4

10-12

4-1 Basic Operation (Until Observation of Specimen)...10,11 4-2 Microscope Adjustments (How to Improve the Observed Image)...11,12

5

1 Adjusting the Interpupillary Distance... 11

2 Adjusting the Diopter ... 11

3 Adjusting the Centering...12

4 Adjusting the Contrast...12

USING THE CONTROLS

13-26

5-1 Base...13-16 1 Adjusting the Brightness...13

2 Using the Light Intensity Preset Switch...13

3 Using the ECO/NORMAL Switch...14

4 Using the Filters... 14-16

5-2 Focusing Block... 17,18 1 Focusing Controls... 17

2 Adjusting the Focus... 17

3 Replacing the Fine Adjustment 4 Adjusting the Coarse Adjustment Knob... 18 Knob Tension... 18 5 Pre-focusing Lever... 18

5-3 Stage...19-21 1 Placing the Specimen...19

2 Using the Auxiliary Slide Holder...19

3 Adjusting the X- and Y-Axis Knob Tension... 20 5 Adjusting the Stage Height... 21

4 Rotating the Stage... 21

5-4 Observation Tube... 22-24 1 Adjusting the Diopter... 22

2 Using the Eye Shades... 22

3 Mounting the Eyepiece Micrometer 4 Selecting the Light Path of the Disk... 23 Trinocular Tube... 23 5 Adjusting the Tilt... 24 6 Using Eyepieces Incorporating a Micrometer... 25

5-5 Condenser... 25 1 Compatibility of Objectives and Condensers... 25

5-6 Immersion Objectives... 26 1 Using Immersion Objectives... 26

5-7 Objectives with Correction Collar... 26

6

CAMERA RECORDING

7

TROUBLESHOOTING GUIDE

28-30

8

SPECIFICATIONS

31,32

9

OPTICAL CHARACTERISTICS (UIS2 Series)

10

ASSEMBLY -- See this section for the replacement of the light bulb. --

34-37

11

PREVENTIVE INSPECTION SHEET FOR ILLUMINATION DEVICES

38

27

33

n PROPER SELECTION OF THE POWER SUPPLY CORD... 39,40

BX53

IMPORTANT This microscope employs a UIS2 (Universal Infinity System) optical design, and should be used only with UIS2 eyepieces, objectives and condensers for the BX2 series. (Some of the modules designed for the BX series and objectives/eyepieces for the UIS series are also usable. For details, please consult Olympus or the catalogues.) Less than optimum performance may result if inappropriate accessories are used.

SAFETY PRECAUTIONS

(Fig. 1) 1. If potentially infectious samples may be observed, use protective gloves or other protective means to prevent the skin from contacting with samples directly. After observation, be sure to clean the portion contacted with samples. · Moving this product is accompanied with the risk of dropping the sample. Be sure to remove the sample before moving this product. · In case the sample is damaged by erroneous operation, promptly take the infection prevention measures. · Follow the procedures described in Chapter “Getting Ready” (see on page 2) prior to using the accessories of this product. Otherwise, the stability of the device will be lost and the dropped sample will cause the possibility of infection. · When you maintain the device which may have contacted with potentially infectious reagents, be sure to wear the protectors such as gloves, or clean the device prior to operation. · Before disposing of device contacted with potentially infectious samples, be sure to follow the regulations and rules of your local government. 2. Install the microscope on a sturdy, level table or bench. In particular, do not put the mat or so under the microscope for safety purpose. 3. Be careful not to tilt the microscope too much. Otherwise, the mobile parts such as the light path selector knob may move in unintended directions. 4. The surfaces of the lamp housing on the rear of the microscope will become extremely hot during operation. When installing the microscope, make sure to allow ample free space around and in particular above the halogen lamp housing. After the microscope is installed, make sure that the space under the lamp housing is ensured. 5. When installing the microscope, route the power cord away from the lamp housing. Should the power cord come in contact with the hot lamp housing, the power cord could melt and cause electric shock. 6. To avoid potential shock hazards and burns when replacing the light bulb, set the main switch @ to “ ” (OFF) then disconnect the power cord from the wall outlet in advance. Whenever you replace the bulb during use or right after use, allow the lamp housing, and bulb to cool before touching. (Fig. 1)

1

Designated bulb CAUTION

Fig. 1

12V100WHAL-L (PHILIPS 7724)

Always use the designated lamp bulb. Using other bulb may cause malfunction.

7. Always use the power cord provided by Olympus. If no power cord is provided, please select the proper power cord by referring to the section “PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of this instruction manual. If the proper power cord is not used, product safety performance cannot be warranted. 8. Always ensure that the grounding terminal of the microscope and that of the wall outlet are properly connected. If the equipment is not grounded, Olympus can no longer warrant the electrical safety performance of the equipment. 9. Never insert metallic objects into the air vents of the microscope frame as this could result in electrical shock, personal injury and equipment damage. 10. The standard service life of the lamp housing is eight (8) years of use or 20,000 hours of total power ON period, whichever is the shorter period. For details, see Inspection Sheet on page 38.

1

Restrictions in Use When all combinations (@ to ³) below are satisfied at the same time, the microscope may fall if tilted larger than a certain degree, comparing to other combinations. Please mount the camera port of the intermediate observation tube toward diagonally backward 45° to prevent from falling. @ The observation tube is either the trinocular tube, U-TTBI, U-ETBI or U-TTLBI. ² The intermediate observation tube is either U-TRUS or U-DP. ³ The camera heavier than 0.4kg is mounted on the intermediate observation tube described in ².

Safety Symbols The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment in the safest possible manner. Symbol

Explanation Indicates that the surface becomes hot, and should not be touched with bare hands. Indicates a non-specific general hazard. Follow the description given after this symbol or in instruction manual. Indicates that the main switch is ON. Indicates that the main switch is OFF.

Caution indications Caution labels are placed at parts where special precaution is required when handling and using the microscope. Always heed the cautions. Caution label position

Lamp housing [Caution against high temperature]

Caution label position

Rear panel [Caution against high temperature]

If a caution label is dirty or peeled off, contact Olympus for the replacement or other inquiry.

1

Getting Ready

(Fig. 2) 1. A microscope is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact. Also note that the microscope does not have a waterproof construction. 2. Do not use the microscope where it is subjected to direct sunlight, high temperature and humidity, dust or vibrations. (For the operating conditions, refer to section 8, “SPECIFICATIONS”.) 3. When moving the microscope, remove the observation and specimen and carefully carry the microscope by holding the grasping part on the rear of the arm and the front part of the base as shown in Fig. 2. CAUTION

Fig. 2

2

To prevent damage to the microscope, never hold it by the stage or coarse/fine adjustment knob.

· Even when moving the microscope along the desktop surface, be sure to remove the specimen in advance. · When the microscope needs to be packaged for forwarding to a remote location, contact Olympus. Please do not lift up the microscope by holding the bottom side of the power supply cover (black) located on the rear panel. 4. When the accessories of this product are used, make sure that the system height does not exceed 1 meter. Otherwise, the stability of the device will be lost. 5. When an arm, illuminator, intermediate attachment, observation tube and camera are to be attached to the upper part of the microscope frame, make sure that their total weight does not exceed 18 kg.

BX53

2

Maintenance and Storage 1. To clean the lenses and other glass components, simply blow dirt away using a commercially available blower and wipe gently using a piece of cleaning paper (or clean gauze). If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available absolute alcohol.

CAUTION

· Since the absolute alcohol is highly flammable, it must be handled carefully. Be sure to keep it away from open flames or potential sources of electrical sparks --- for example, electrical equipment that is being switched on or off, which could cause ignition of a fire. Also remember to always use it only in a well-ventilated room. · Set the main switch to “ ” (OFF) and wait for the lamp housing to cool down sufficiently before proceeding to maintenance.

2. Do not use organic solvents to clean the microscope components other than the glass components. To clean them, use a lint-free, soft cloth slightly moistened with a diluted neutral detergent. 3. Do not disassemble any part of the microscope except for the parts that are specified to be disassembled in this manual, as this could result in malfunction or reduced performance. 4. After using the microscope, set the main switch to “ ” (OFF), wait for the lamp housing to cool down sufficiently, and keep it covered with a dust cover during storage. 5. Before disposing of this product, be sure to follow the regulations and rules of your local government.

3

Caution If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the equipment may also be damaged. Always use the equipment as outlined in this instruction manual. The following symbols are used to set off text in this instruction manual. CAUTION : Indicates a potentially hazardous situation which, if not avoided, may result in minor

}

4

or moderate injury or damage to the equipment or other property. It may also be used to alert against unsafe practices. : Indicates commentary (for ease of operation and maintenance).

Intended use This product has been designed to be used to observe magnified images of specimens in various routine work and research applications. This includes the observation of living cells or of specimen taken from tissues to gain physiological or morphological information at hospitals or laboratories. Typical field of applications are genetics, human blood and tissue examination, neurology, pharmacology and cellular biology. Do not use this instrument for any purpose other than its intended use. This product complies with the requirements of directive 98/79/EC concerning in vitro diagnostic medical devices. CE marking means the conformity to the directive.

Safety and EMC Precautions · This product complies with the emission and immunity requirements described in IEC61326 series. · The electromagnetic environment should be evaluated prior to operation of this product. Do not use this product in close proximity to the sources of strong electromagnetic radiation to prevent interference with the proper operation. · Use only power cord which OLYMPUS specifies. Otherwise the safety and EMC performance of the product can not be assured. · Be sure to ground the product. Otherwise our intended electric safety and EMC performance of the product can not be assured.

3

1 MODULE NOMENCLATURE } The modules mentioned below show only the typical product names. As there are some products that are not mentioned but also applicable to this microscope, check the latest catalogues or consult Olympus. For the products marked “ * ”, also read their instruction manuals. Observation tube · U-BI30-2 · U-TBI-3 · U-TR30-2 · U-TTLBI Eyepieces · WHN10X · WHN10X-H

Intermediate attachment · U-TRU · U-CA · U-DO3

Arm (or vertical illuminator) · BX3-ARM · BX3-URA* · BX3-RFAS* · BX3-RFAA*

Lamp housing · U-LH100-3 Revolving nosepiece · U-D6RE · U-D7RE · U-D7RES · U-D7REA

Slide holder · U-HLST-4 · U-HLD-4 · U-HLDT-4

Stage · U-SVRB-4 · U-SVRO

Condenser · U-SC3 · U-AAC · U-UCD8 · BX3-UCD8A

4

2 CONTROLS

BX53

} If you have not yet assembled the microscope, read section 10, “ASSEMBLY” (pages 34 to 37).

Interpupillary distance adjustment scale (Page 11)

Light path selector knob (Page 23)

Condenser height adjustment knob (Page 12)

Diopter adjustment ring (Page 22)

Slide holder (Page 19)

Aperture iris diaphragm ring (Page 12)

Filter mount (Page 15)

Field iris diaphragm ring (Page 12)

Allen screwdriver holder Coarse adjustment knob (Page 17)

Y-axis knob* (Page 20) X-axis knob* (Page 20)

Light preset adjustment screw (Page 13) Coarse adjustment tension adjustment ring (Page 18)

Filter knobs (Page 15) ND6, ND25 and LBD from the front to the rear (In-out possible from the other side)

* The U-SHG (thin type) and U-SHGT (thick type) stage feed knob rubber caps can be attached optionally.

5

Main switch (Page 10) I : ON : OFF Pre-focusing lever (Page 18) Pilot LED Fine adjustment knob (Page 17)

Green lighting: Lamp bulb ON Red lighting: Auto OFF

Detachable. Human sensor (Page 14)

Light intensity preset switch (Page 13) Coarse adjustment knob (Page 17)

Brightness adjustment knob (Page 13)

Filter knobs (Page 15) ND6, ND25 and LBD from the front to the rear (In-out possible from the other side) ECO indicator (Page 14) ECO: ON (Green) NORMAL: OFF

6

ECO/NORMAL switch (Page 14)

BX53

Low Magnification Condenser U-LC

Swing-out Condenser U-SC3 Top lens

Auxiliary lens mounting tool Top lens swing-out knob

Aperture iris diaphragm ring (Page 12) Auxiliary lens for U-LC

Condenser mount dovetail

* The auxiliary lens is mounted on the lamp adapter when the U-LC is used. (Page 35)

7

3 FLOW OF OBSERVATION } When the ECO mode is set, the halogen bulb turns OFF automatically in about 30 minutes the microscope becomes unattended.

Set the main switch to “ I ” (ON) and adjust the brightness.

Engage only the LBD filter.

Select the light path (trinocular tube).

Place the specimen on the stage.

Engage the 10X objective in the light path.

Bring the specimen in focus.

(Page)

@Main switch 2Brightness adjustment knob

(P. 13)

3LBD filter knob

(P. 10,14-16)

4Light path selector knob

(P. 23)

5Slide holder 6X-/Y-axis knobs

(P. 19) (P. 20)

7Revolving nosepiece

8Coarse/fine adjustment knobs

(P. 17)

9Binocular tube aDiopter adjustment ring bCondenser height adjustment knob cCondenser centering knob

(P. 11) (P. 22) (P. 12)

Adjust the aperture iris and field iris diaphragms.

dAperture iris diaphragm ring eField iris diaphragm ring

(P. 12) (P. 12)

Engage the objective to be used in the light path and bring the specimen in focus.

7Revolving nosepiece 8Coarse/fine adjustment knobs

(P. 17)

Adjust the interpupillary distance. Adjust the diopter. Adjust the light axis.

Engage the required filters.

Adjust the brightness.

Start observation.

8

(Controls Used)

fFilters

2Brightness adjustment knob

(P. 12)

(P. 10,14-16)

(P. 13)

BX53

9 4

1

b a

7 5

d

6 c e 3 2

f 8

} Copy the observation procedure pages on separate sheets and post it near your microscope.

9

4 SIMPLIFIED OBSERVATION PROCEDURE 4-1 Basic Operation (Until Observation of Specimen) This section describes the basic operation of the microscope until the start of observation of a specimen. For the detailed operating procedure of each control, please read the description page specified below.

1

Press the main switch of the microscope frame to “ I ” (ON).

2

Rotate the knob to adjust the lamp brightness. (Details: Page 13)

3

Push in the LBD filter knob to engage the LBD filter in the light path. (Details: Page 15)

1

Fig. 3

2

Fig. 4

3

Fig. 5

What is LBD filter? The color of the sun at the noon of a clear day is called the daylight color, which looks white to human eyes. On the other hand, the halogen bulb used as the light source of this microscope does not emit the daylight-color light, so the LBD filter is engaged in the light path to convert the color of halogen bulb light into daylight. In general, engaging the LBD filter makes it possible to observe specimens in natural color tones.

10

BX53

5

4

Rotate the knob to lower the stage. (Details: Page 17)

5

Open the clamping lever of the slide holder and place the specimen slide. (Details: Page 19)

6

Rotate the revolving nosepiece to engage the 10X objective in the light path.

7

Rotate the coarse and fine adjustment knobs to bring the specimen into focus. (Details: Page 17)

8

Rotate the stage knob to adjust the observation position.

4 Fig. 6

6

7

8 Now you can observe the magnified image of the specimen. To improve the observation further, read section 4-2, “Microscope Adjustments” below. Fig. 7

4-2 Microscope Adjustments (How to Improve the Observed Image) Adjust the microscope as described below to improve the observed image.

1

Adjusting the Interpupillary Distance 1

While looking through the eyepieces, adjust for binocular vision until the left and right fields of view coincide completely. } Note your interpupillary distance so that it can be quickly duplicated.

1

Fig. 8

2

Adjusting the Diopter The diopter adjustment ensures parfocality from low to high magnifications and makes it possible to avoid defocusing even when the objective is switched. For details, see page 22.

Fig. 9

11

3

Adjusting the Centering 1 2

4

7

6

5 3 8

9

1

Place the specimen.

2

Rotate the revolving nosepiece to select the 10X objective.

3

Rotate the knobs to bring the specimen into focus.

4

Rotate the knob to raise the condenser to its upper limit.

5

Rotate the field iris diaphragm ring in the direction of the arrow so that the diaphragm image comes inside the field of view.

6

Rotate the knobs to focus on the field iris diaphragm image (A in Fig. 10).

7

Turn the two condenser centering screws to move the iris diaphragm image to the center of the field of view (B in Fig. 10).

8

Gradually open the field iris diaphragm until its image is inscribed in the field of view (C in Fig. 10).

9

Open the field diaphragm slightly until its image circumscribes the field of view slightly (D in Fig. 10).

} Be careful not to rotate the condenser clamping screw, located behind the centering knobs, by mistake. } When using a condenser with top lens such as the U-SC3, engage the top lens in the light path before proceeding to the centering adjustment.

A

B Fig. 10

4

C

D

Movement of Field Iris Diaphragm Image

Adjusting the Contrast Aperture iris diaphragm image

70-80% 30-20%

Objective pupil N.A. scale

12

2

1

1

Set the condenser aperture iris diaphragm ring so that the condenser NA scale position indicates 70% of the NA of the objective in use. After this, observe the image and fine adjust the aperture iris diaphragm to the desired condition.

2

The image of the aperture iris diaphragm can be viewed by removing the eyepieces and looking into the observation tube. Adjust so that the image looks approximately like that shown in the figure above.

5 USING THE CONTROLS

BX53

5-1 Base 1

Adjusting the Brightness

(Fig. 11)

Turn the brightness adjustment knob @ clockwise to increase the voltage and make illumination brighter. } When the brightness adjustment knob @ is adjusted to the camera symbol marking, the brightness of the light becomes the daylight (color temperature of about 5500K). However, note that the daylight may not always be reproduced accurately depending on the degree of bulb consumption, etc.

1

Fig. 11

2 1 2

Fig. 12

Using the Light Intensity Preset Switch

(Fig. 12)

The light intensity preset switch @ makes it possible to limit the light intensity to a preselected level regardless of the position of the brightness adjustment knob. The light intensity preset has been preset to optimum brightness for photography when the built-in LBD filter is engaged (approx. 9 V with marking). 1. Press the light intensity preset switch @ to the ON position. (The face of the switch lights when it is ON.) 2. Using a small flat-blade screwdriver, turn the preset adjustment screw 2 to obtain the required light intensity. Turning the screw clockwise increases brightness. 3. When the light intensity preset switch @ is set to OFF, the brightness returns to the level set by the brightness adjustment knob. } While the light intensity preset switch is ON, turning the light brightness adjustment knob does not affect brightness.

13

3

5 4

(Fig. 13)

} The ECO mode helps prevent leaving the halogen bulb ON while the microscope is not used. 1. Press the ECO/NORMAL switch 3 to ON (so that the PILOT lamp | lights in green). Hereafter, when the human sensor 5 of the microscope has not detected human motion, etc. for more than about 30 minutes, the lighting halogen bulb is turned OFF automatically (the PILOT lamp | lights in red). 2. To turn the bulb ON again after it has been turned OFF automatically: · Set the main switch to “ ” (OFF) then “ I ” (ON) again. · Press the ECO/NORMAL switch 3 twice.

1 2

3

Using the ECO/NORMAL Switch

Fig. 13

} In the ECO mode, the human sensor may sometimes be unable to detect a human body and the halogen bulb be turned OFF during use of the microscope in the following cases. · When the heat source (human skin, etc.) in the detection range of the human sensor hardly moves or moves at a high speed. · When far-IR rays from the sun or incandescent lamp is directly incident on the human sensor. · When an object that transmits little far-IR rays, such as glass or acrylic plate, is located between the human sensor and the person operating the microscope. · When there is little difference between the ambient temperature and the body temperature of the person. } In the ECO mode, the halogen bulb may not be turned OFF automatically in the following cases. In addition to these cases, the halogen bulb may neither be turned OFF in the case of sensor detection error. · When the sensor detects the movement of a heat source other than human body. · When the sensor detects a heat source that repeats heat generation, such as a motor repeating ON-OFF. · When the temperature in the detection area changes suddenly, for example due to hot/cold winds of an air conditioner or vapor from a room humidifier.

4

Using the Filters

(Figs. 14 to 20)

} You can place a filter in the light path with one of the following methods. · Push in the knob of the desired built-in filter to engage it in the light path. (Page 15) · Place a filter on the filter mount on the base and engage in the light path. (Page 15) · Insert a filter in the U-FC filter cassette, attach it on the filter mount and engage it in the light path by sliding the filter lever. (Page 15)

14

BX53

Using Built-In Filters (Figs. 14 & 15) Use the filter knobs to engage or disengage the corresponding filters into or from the light path. Filter Type/Purpose

1

2

3

Fig. 14

IN OUT

OUT IN

1

ND6 (Neutral Density filter for light adjustment, transmittance 6%)

2

ND25 (Neutral Density filter for light adjustment, transmittance 25%)

3

LBD (for color balancing, daylight filter)

Assuming that you face the microscope from the front panel, push in a filter knob on the right or pull out a filter knob on the left to engage the corresponding filter in the light path. The filter can be disengaged from the light path by reversing the above. } When pushing in a filter knob, push it all the way to ensure engagement of the corresponding filter. If the filter is engaged incompletely, optimum observation may be impossible. Mounting a Single Filter (Fig. 16) A filter with a diameter of 45 mm 5 can be placed on the filter mount on the base. If you need to place multiple filters, please purchase the U-FC filter cassette.

Fig. 15

CAUTION

Even when a filter cassette is used, a filter with thickness of no more than 3 mm can still be placed on the filter mount.

Using the Filter Cassette (Figs. 17 to 20) Loading Filters into Filter Cassette

5

} The filter cassette accommodates filters with a diameter of 45 mm and thickness of 2.7 mm or less. } The filter cassette has two filter levels on the right side and one on the left side. } When the filter cassette is used, the ECO mode cannot be used because the human sensor is hidden by the filter cassette. 1. Move all filter levers to the OUT position except for the one belonging to the slot into which the filter is to be inserted. 2. Slide the lever 6 to the IN position. Make sure that it clicks securely into place. (Fig. 17) 3. Holding the lever in the position shown in Fig. 17, put the filter into the cassette by inserting it in the direction indicated by the arrow. 4. Insert the other two filters in the same manner.

Fig. 16

6

Fig. 17

15

Mounting the Filter Cassette

8 7 9 a

1. Fully loosen the filter cassette clamping screw †. (Fig. 18) 2. Align the key ‡ on the bottom surface of the filter cassette with the positioning slot 9 on the filter mount, then snap the filter cassette into place from above. 3. Rotate the filter cassette to align its sides with the base. (Fig. 19) 4. Align the clamping screw † with the positioning hole ‰ on the filter mount, then tighten the screw to fasten the filter cassette. CAUTION

Fig. 18

When the filter cassette is installed, the stage may hit it when lowered. Therefore, exercise caution when lowering the stage with the filter cassette installed.

Fig. 19 Using the Filter Cassette (Fig. 20) Usable Filters

b Fig. 20

16

Applications

45-ND6, 45-ND25

Neutral density filter

45G-530, 43IF550-W45

Green

45O-560

Orange

B&W contrast filters

Up to three of the above filters can be loaded in the filter cassette. Moving the levers b on the left and right sides of the cassette to the IN position brings the corresponding filter into the light path.