CKX41 and CKX31 CULTUREMICROSCOPE Instructions Sept 2004

CKX41/CKX31

CONTENTS IMPORTANT - Be sure to read this section for safe use of the equipment. -

1-3

1

NOMENCLATURE

4-5

2

CONTROLS

6-7

3

SUMMARY OF OBSERVATION PROCEDURE

4

USING THE CONTROLS

8

9-15

4-1 Microscope Frame ... 9 1 Turning On the Light Source

2 Adjusting the Brightness

3 Adjusting the Tension of the Coarse Adjustment Knob

4-2 Stage... 10 1 Placing the Specimen

2 Moving the Specimen

4-3 Observation Tube ... 11-14 1 Adjusting the Interpupillary Distance

2 Adjusting the Diopter

3 Using the Eye Shades

4 Using Eyepiece Micrometer Disks

5 Selecting the Light Path (U-TR30-2 Only)

6 Adjusting the Tilt

4-4 Illumination Column... 15 1 Using the Filters

2 Using the Aperture Iris Diaphragm

3 Removing the Condenser Lens

4-5 Objective Correction Collars ... 16 1 Correction Collar

5

PHASE CONTRAST OBSERVATION

6

PHOTOMICROGRAPHY & TV OBSERVATION

7

TROUBLESHOOTING GUIDE

21-22

8

SPECIFICATIONS

23-24

9

ASSEMBLY - See this section for the replacement of the light bulb. -

25-29

17-19

20

PROPER SELECTION OF THE POWER SUPPLY CORD... 30-31

CKX41/CKX31

IMPORTANT The difference between the CKX31 and CKX41 microscopes lie in the following basis systems. CKX31

CKX41

Observation tube

Binocular tube fixed

Replaceable*

Stage center plate

–

Replaceable**

Not mountable

Mountable

Reflected fluorescence system

* The U-CBI30-2/U-BI30-2/U-CTBI/CKX-TBI binocular tube or U-CTR30-2/U-TR30-2 trinocular tube can be mounted. But no intermediate attachment can be mounted. **The standard stage center plate can be replaced with the CK40-CPG glass center plate, IX-CP50 center plate (50 mm), etc.

SAFETY PRECAUTIONS ³

²

@

Fig. 1

5

4 Fig. 2

1. After the equipment has been used in an observation of a specimen that is accompanied with a potential of infection, clean the parts coming in contact with the specimen to prevent infection. · Moving this product is accompanied with the risk of dropping the specimen. Be sure to remove the specimen before moving this product. · In case the specimen is damaged by erroneous operation, promptly take the infection prevention measures. 2. Install the microscope on a sturdy, level table or bench (Weight: CKX31 about 8 kg, CKX41 about 8.8 kg). 3. When transporting the microscope, be sure to keep it horizontal by holding it by the root of the observation tube relay tube 1 and the illumination column 2. (Fig. 1) Be sure to remove the specimen since it may fall. # Do not tilt it during carrying to prevent damaging the desktop or microscope. 4. Culture liquid or water spilt on the stage, objective or microscope frame may damage the equipment. Immediately disconnect the power cord from the wall outlet and wipe the liquid or water off if it is spilt on them. 5. The surfaces of the lamp socket 3 of the illumination column will become extremely hot during operation. When installing the microscope, make sure to allow ample free space around and in particular above the lamp socket. (Fig. 1) When using the TV camera or photomicrographic system, install them so that the connection cable does not come in contact with the lamp socket. 6. To avoid potential shock hazards and burns when replacing the light bulb, set the main switch 4 to “ ” (OFF) then disconnect the power cord from the wall outlet in advance. Whenever you replace the bulb during use or right after use, allow the lamp socket 3 and bulb to cool before touching. (Figs. 1 & 2) Designated bulbs:

6V30WHAL high-intensity halogen bulb (PHILIPS 5761)

7. Always use the power cord provided by Olympus. If no power cord is provided, please select the proper power cord by referring to the section “PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of this instruction manual. If the proper power cord is not used, product safety performance cannot be warranted. 8. Always ensure that the grounding terminal of the microscope and that of the wall outlet are properly connected. If the equipment is not grounded, Olympus can no longer warrant the electrical safety performance of the equipment.

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9. The microscope system is unstable when the large camera back is attached. When pulling out the film, be sure to hold the microscope with one hand. 10. Always turn the light intensity control knob 5 gently. Do not attempt to turn it beyond the stop position. (Fig. 2)

Safety Symbols The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment in the safest possible manner. Symbol

Explanation Indicates that the surface becomes hot, and should not be touched with bare hands. Before use, carefully read the instruction manual. Improper use could result in personal injury to the user and/or damage to the equipment. Indicates that the main switch is ON.

Indicates that the main switch is OFF.

Warnings Warning engraving is placed at parts where special precaution is required when handling and using the microscope. Always heed the warnings. Warning engraving position

1

Lamp socket (U-LS30-3) [Warning against high temperature]

Getting Ready 1. A microscope is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact. 2. Do not use the microscope where it is subjected to direct sunlight, high temperature and humidity, dust or vibrations. (For the operating conditions, see chapter 8, “SPECIFICATIONS”.) 3. Use the tension adjustment ring to adjust the rotation tension of the coarse adjustment knob. 4. For the applicable objectives, see chapter 8, “SPECIFICATIONS”. Olympus cannot guarantee the performance when other objective than specified is used.

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CKX41/CKX31

2

Maintenance and Storage 1. Clean all glass components by wiping gently with gauze. To remove fingerprints or oil smudges, wipe with gauze slightly moistened with a mixture of ether (70%) and alcohol (30%). Since solvents such as ether and alcohol are highly flammable, they must be handled carefully. Be sure to keep these chemicals away from open flames or potential sources of electrical sparks –– for example, electrical equipment that is being switched on or off. Also remember to always use these chemicals only in a well-ventilated room. 2. Do not attempt to use organic solvents to clean the microscope components other than the glass components. To clean them, use a lint-free, soft cloth slightly moistened with a diluted neutral detergent. 3. Be careful not to spill any liquid –– such as a culture solution –– on the unit. If you do spill anything, immediately set the main switch to “ ” (OFF) and unplug the power cord. Then wipe away any liquid on or under the objectives. 4. If no objectives are mounted, be sure to cover the objective mounting threaded positions on the revolving nosepiece to prevent and dust and spilled culture solution from getting on the lenses inside. 5. Do not disassemble any part of the microscope as this could result in malfunction or reduced performance. 6. When not using the microscope, keep it covered with a dust cover. Make sure the lamp socket is cool before covering the microscope. 7. Using a device that radiates ultraviolet light such as a germicidal lamp near the microscope may discolor (yellow) parts of the microscope surface. The amount of discoloration depends on the radiation intensity of the ultraviolet light and the distance between the microscope and radiation source. When not using the microscope, cover it with the dust cover. We recommend that you also cover the microscope with an impermeable sheet.

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Caution If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the equipment may also be damaged. Always use the equipment as outlined in this instruction manual. The following symbols are used to set off text in this instruction manual. : Indicates that failure to follow the instructions in the warning could result in bodily harm to the user and/or damage to equipment (including objects in the vicinity of the equipment). # : Indicates that failure to follow the instructions could result in damage to equipment. } : Indicates commentary (for ease of operation and maintenance).

This device complies with the requirements of directive 98/79/EC concerning in vitro diagnostic medical devices. CE marking means the conformity to the directive.

NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment. This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the instruction manual, may cause harmful interference to radio communications. Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense. FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment.

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NOMENCLATURE CKX31

Lamp Socket

Phase Contrast Slider

U-LS30-3

· Pre-centered Ph slider: IX2-SLP · Ph centering slider: IX2-SL

Objectives For brightfield observation: PlanCN 4X PlanCN 10X PlanCN 20X PlanCN 40X PlanCN 60X PlanCN 100XO #Dedicated objectives are required for phase contrast observations (see page 17).

Illumination Column (Fixed)

Eyepieces (10X)

Binocular Tube (Fixed) Ultralong Working Distance Condenser

Microscope Frame · CKX31SF

Revolving Nosepiece (Fixed) Quadruple revolving nosepiece

Stage (Fixed) · Stage extension plate: CK2-SS · Mechanical stage: CK40-MVR

4

CKX41/CKX31

CKX41 Lamp Socket

Phase Contrast Slider

U-LS30-3

· Pre-centered Ph slider: IX2-SLP · Ph centering slider: IX2-SL

Objectives

Illumination Column (Fixed)

For brightfield observation: PlanCN 4X PlanCN 10X PlanCN 20X PlanCN 40X PlanCN 60X PlanCN 100XO #Dedicated objectives are required for phase contrast observations (see page 17).

Ultralong Working Distance Condenser

Eyepieces · WHB10X* · WHN10X/WHN10X-H**

Stage Center Plate · Standard stage center plate · Glass stage center plate: CK-40CPG · Stage center plate (50 mm): IX-CP50

Observation Tube · Binocular tube: U-CBI30-2*/U-BI30-2** · Trinocular tube: U-CTR30-2*/U-TR30-2** · Tilting binocular tube: U-CTBI***/CKX-TBI* */** Only the eyepieces carrying the same marking “ * " or “ ** " as the observation tube marking can be attached. *** Dedicated 10X eyepiece built in.

Revolving Nosepiece (Fixed) Quadruple revolving nosepiece

Microscope Frame CKX41SF

Stage (Fixed) · Stage extension plate: CK2-SS · Mechanical stage: CK40-MVR

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CONTROLS CKX31 }If you have not yet assembled the microscope, read chapter 9, “ASSEMBLY” (pages 25 to 29).

Filter holder (Page 29) Phase contrast slider (Page 16)

Aperture iris diaphragm lever (Page 15)

Interpupillary distance scale (Page 11) Slider centering knob storage holes Diopter adjustment ring (Page 12)

Light intensity control knob (Page 9)

Fine adjustment knob Coarse adjustment knob Coarse adjustment knob tension adjustment ring (Page 9) Main switch (Page 9)

6

CKX41/CKX31

CKX41 }If you have not yet assembled the microscope, read chapter 9, “ASSEMBLY” (pages 25 to 29).

Filter holder (Page 29)

Interpupillary distance scale (Page 11)

Phase contrast slider (Page 16)

Aperture iris diaphragm lever (Page 15)

Diopter adjustment ring (Page 12)

Standard stage center plate

Slider centering knob storage holes

Light intensity control knob (Page 9)

Fine adjustment knob Coarse adjustment knob Coarse adjustment knob tension adjustment ring (Page 9) Main switch (Page 9)

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SUMMARY OF OBSERVATION PROCEDURE

1. Set the main switch 1 to “ I ” (ON) and turn the light intensity control knob 2 to obtain appropriate brightness. (Page 9)

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@ 2. When using the U-TR30-2 trinocular tube, push in the light path selector knob 3 to set the light path at 100% for binocular observation. (Page 14)

³ 3. Place a specimen on the stage. (Page 10) 4. Turn the revolving nosepiece to bring the 10X objective into the light path. Be sure to turn the revolving nosepiece until it clicks.

5. Adjust the interpupillary distance of the eyepieces. (Page 11)

6. Adjust the diopter of the eyepieces. (Page 12)

7. Bring the required objective into the light path and focus on the specimen. 8. When using the 40X objective provided with the correction collar, set the scale on the correction collar according to the thickness of the vessel bottom. (Page 16) }When performing phase contrast observation, see pages 17 and after.

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9. When observing an undyed specimen with brightfield, stop down the aperture iris diaphragm 4. In phase contrast observation, set the aperture wide open. (Page 15) Bring the required filter into the light path. (Page 15) 10. In brightfield observation, use the LBD filter. In phase contrast observation, use the IF550 green filter as required. }In photomicrography, it is recommended to use the 45HA heat absorbing filter.

CKX41/CKX31

USING THE CONTROLS 4-1 Microscope Frame 1

Turning On the Light Source

(Fig. 3)

Set the main switch 1 on the side panel of the microscope frame to “ I ” (ON).

@ Fig. 3

2

Adjusting the Brightness

(Fig. 4)

Turn the light intensity control 1 clockwise to raise the voltage and increase the brightness. Turn it counterclockwise to lower the voltage and decrease the brightness. }The service life of the bulb can be extended by using the bulb at a lower voltage.

@

Fig. 4

the Tension of the Coarse 3 Adjusting Adjustment Knob

(Fig. 5)

# Be sure to use the tension adjustment ring 1 to adjust the rotation tension of the coarse adjustment knob. Adjustment Procedure

@ Fig. 5

Turn the tension adjustment ring 1 with your fingers or using a flat-blade screwdriver. When the ring is turned in the direction of the arrow, tension of the coarse adjustment knob increases. Turning the ring in the opposite direction decreases the tension. If the revolving nosepiece descends on its own or if the specimen gets out of focus quickly even when it is brought into focus using the fine adjustment knob, it means that the tension of the coarse adjustment knob is too low. Turn the ring in the direction of the arrow to increase the tension.

9

4-2 Stage 1

@

Placing the Specimen

(Figs. 6 & 7)

Put the specimen in the center of the stage. When Using a

35 mm Petri Dish

}With the CKX41, a 35 mm petri dish can be mounted directly on the stage provided that the standard stage center plate is in use. 1. With the CKX31, put the provided 35 mm petri dish holder 1 on the stage and mount the 35 mm petri dish on the opening in the center. 2. To move the petri dish, slide the entire holder. Fig. 6

Using the Mechanical Stage 1. When using a 96-well or 24-well micro-titer plate, extend the specimen holder 2 to directly hold the micro-titer plate. (Fig. 7) 2. To hold any other type of plate, combine one of the following provided holders with the mechanical stage. · Terasaki holder 3 (AB4488): For Terasaki plate, 35 mm petri dish holder 4 or 65 mm petri dish. · Slide glass holder 5 (AB4489): For slide glass, 54 mm petri dish. · Blood cell test plate holder IX2-BCTP 6 (optional):For a blood cell test plate holder or other calculating chamber for bacteria and eosinophil +0.3 +0.3 with mounting section dimensions corresponding to H 77 0 x V 35 0 x D 2 mm, or for a 60mm petri dish. 3. The specimen can be moved to the desired position by turning the X-axis knob 7 and Y-axis knob 8 (Stroke: 120 mm in X-axis direction, 78 mm in Y-axis direction).

| ² ³ 5

6

8 7

Fig. 7

2

Moving the Specimen Turn the X-axis and Y-axis knobs of the mechanical stage or move the specimen directly by hand. # Be careful when changing objectives. When objectives are switched after observing the specimen with an objective with short working distance, the newly selected objective may interfere with the stage center plate or petri dish holder. }With the CKX41, the IX-CP50 stage center plate ( 50 mm) allows a wide range of use without interference.

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CKX41/CKX31

4-3 Observation Tube 1

Adjusting the Interpupillary Distance

(Figs. 8 to 10)

When adjusting the interpupillary distance, take care not to let your fingers get caught in the gaps of the binocular section. With the CKX31 While looking through the eyepieces, move both eyepieces until the left and right fields of view coincide completely. Adjust so that the two index dots · 1 are horizontal. (Fig. 9) Fig. 8 }To make the line connecting the two index dots horizontal, adjust so that the index dots come on the extension of one of the horizontality lines inscribed on the pivot. When your interpupillary distance is other than 50, 60, 70 and 75, adjust so that the line connecting the two index dots are in parallel with the horizontality lines on the pivot. (Fig. 9) }Note your interpupillary distance so that it can be quickly duplicated.

@ Fig. 9 With the CKX41 }When the observation tube is the U-CBI30-2, U-CTR30-2 or U-CTBI, follow the procedure in "With the CKX31". }When the observation tube is the U-BI30-2, U-TR30-2 or CKX-TBI only one index dot is provided. (Fig. 10) · While looking through the eyepieces, move both eyepieces until the left and right fields of view coincide completely. The position of index dot · indicates the interpupillary distance. }Note your interpupillary distance so that it can be quickly duplicated. Fig. 10

11

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2

Adjusting the Diopter

(Figs. 11 to 13)

With the CKX31 1. While looking through the left eyepiece with your left eye, turn the coarse and fine focus adjustment knobs to bring the specimen into focus. 2. While looking through the right eyepiece with your right eye, turn only the diopter adjustment ring 1 to focus on the specimen. (Fig. 11)

Fig. 11 With the CKX41

²

}When the U-CTBI is used, align the white dot · with the index line on the scale of the right eyepiece’s diopter adjustment ring. 1. While looking through the right eyepiece with your right eye, turn the coarse and fine focus adjustment knobs to bring the specimen into focus. 2. While looking through the left eyepiece with your left eye, turn only the diopter adjustment ring 2 to focus on the specimen. (Fig. 12)

Fig. 12 Using a Finder Eyepiece }Insert the finder eyepiece into the right eyepiece sleeve of the U-TR30-2 trinocular tube. 1. Looking through the right eyepiece with your right eye, turn the eyepiece top ring until clearly defined double crosslines can be seen in the field of view. (Fig. 13) 2. Looking through the right eyepiece, turn the coarse and fine adjustment knobs to bring the specimen and double crosslines into simultaneous focus. 3. Looking through the left eyepiece with your left eye, turn the diopter adjustment ring to focus on the specimen.

Fig. 13

3

Using the Eye Shades

(Fig. 14)

When Wearing Eyeglasses Use with the eyeshades in the normal, folded-down position. This will prevent the eyeglasses from being scratched. When Not Wearing Eyeglasses Extend the folded eyeshades in the direction of the arrow to prevent extraneous light from entering between the eyepieces and eyes. Fig. 14

12

CKX41/CKX31

4 1

2

WHN10X-H

Fig. 15-1

WHB10X-H

4

3

5 Fig. 15-2

Using the Eyepiece Micrometer Disk

(Figs. 15)

}When the WHN10X-H (or WHN10X) eyepieces are used, an eyepiece micrometer disk can be inserted in one of them. When the eyepiece does not have a diopter adjustment mechanism, however, it is hard to focus on the micrometer disk if the operator has poor eyesight. Should that be the case, adjust the focus with eyeglasses on. Use an eyepiece micrometer disk with a diameter of 24 mm and thickness of 1.5 mm. Following Fig. 15-1, turn the built-in micrometer-mounting frame ² counterclockwise to remove it from the eyepiece and place a micrometer disk @ into the mounting frame. The engraving on the eyepiece micrometer disk should face downward in the micrometer-mounting frame. Screw the micrometer-mounting frame back into the eyepiece. }When the WHB10X-H (or WHB10X) eyepieces are used, an eyepiece micrometer disk with a diameter of 20.4 mm and thickness of 1 mm can be inserted in one of them using the 20.4RH reticle holders ³ (2-piece set). When the eyepiece does not have a diopter adjustment mechanism, however, it is hard to focus on the micrometer disk if the operator has poor eyesight. Should that be the case, adjust the focus with eyeglasses on. When the reticle holders are used, the field number becomes 19.6. The field number of the U-CTBI's built-in eyepieces is 18, so it does not change. 1. Remove both eyepieces. (When the U-CTBI is used, remove only the right eyepiece by unscrewing the clamping screw on it with a precision flat-blade screwdriver.) 2. Place an eyepiece micrometer disk | in one of the reticle holders ³ so that the engraving on the eyepiece micrometer disk faces downward. 3. Screw the reticle holder ³ containing the eyepiece micrometer disk | into the bottom of the eyepiece. At the end of screwing, turn the reticle holder by hooking your nail on its notch 5 to screw it all the way in. 4. To provide the other eyepiece with the same field number, screw in the other reticle holder, without eyepiece micrometer disk, into the bottom of the other eyepiece. 5. Replace the eyepiece(s) in its(their) original position(s).

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5

Selecting the Light Path (U-TR30-2 Only)

(Fig. 16)

Slide the light path selector knob 1 to select the desired light path. Light Path Selector Knob

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Symbol

Intensity Ratio

Applications

Pushed in

100% for binocular eyepieces

Observation of dark specimens

Middle position

20% for binocular Observation of eyepieces, 80% for bright specimens, photography, TV TV/photography observation

Pulled out

100% for TV/ photography

Fig. 16

Photography, TV observation

# The U-CTR30-2 trinocular observation tube does not have the light path selector knob and its light intensity ratio is fixed at 50% binocular and 50% TV/photography.

@

@ Fig. 17

14

6

Adjusting the Tilt

(Fig. 17)

}When using the U-CTBI or CKX-TBI, adjust the height and tilt of the observation tube to obtain the most comfortable viewing position. Holding the binocular section with both hands, raise or lower it to the desired position. · U-CTBI/CKX-TBI: 30 to 60° # Never attempt to force the binocular section past the upper or lower stop position. Applying excessive force could destroy the limiting mechanism. # The connectable eyepieces are the WHB10X only or the CXK-TBI and the built-in eyepieces (10X) for the U-TCBI. Combination with any other eyepiece will result in insufficient illumination at the periphery of the field of view. When adjusting the tilt of the U-CTBI, be careful not to have your fingers caught by the spaces @ between the binocular assembly and cover.

CKX41/CKX31

4-4 Illumination Column 1

Using the Filters }Using appropriate filters according to the purposes allows you to observe and photograph specimens more effectively. Particularly, the use of the LBD filter is recommended in observation and photomicrography because it renders more neutral colors. }More than one filter can be stacked in the filter holder (Filter diameter: 45 mm. Maximum thickness of stacked filters: 11 mm). Filter

Aperture iris diaphragm image

70-80%

2

30-20%

Application

45IF550-W45

Monochrome contrast filter (Green)

45ND6, 45ND25

Light intensity adjustment filter (Transmittance 6% and 25%)

45LBD2-N, 45KB

Color temperature conversion filter (For observation and photomicrography)

45HA (Heat absorbing filter)

Exposure time compensation in photomicrography

Using the Aperture Iris Diaphragm

(Fig. 18)

}The aperture iris diaphragm determines the numerical aperture of the illumination system in brightfield observation. It enables you to adjust the depth of focus, contrast and resolution according to your requirements. · Checking the aperture iris diaphragm: Remove the eyepiece when necessary (and insert the CT-5 or UCT30 if you have one). Then look into the eyepiece sleeve; you will seen the field of view as shown in Fig. 18. Now adjust the aperture iris diaphragm lever as required. · In general, when observing a dyed specimen, set the aperture iris diaphragm to 70% to 80% of the N.A. of the objective in use. However, when observing a culture specimen, which is not dyed, set the aperture iris diaphragm lever toward “ ”.

Objective pupil

Fig. 18

3

Removing the Condenser Lens

(Fig. 19)

}To provide more working distance, turn the condenser’s lower section 1 in the direction of the arrow and remove it. When you do this, however, keep in mind that proper illumination cannot be achieved. Remove the condenser lens only when using a large culture vessel.

@

Fig. 19

15

4-5 Objective Correction Collars 1 @

² Fig. 20

16

Correction Collar

(Fig. 20)

}A culture microscope is designed to observe specimens contained in vessels of various bottom thickness values. In order to achieve optimum objective performance of the culture microscope, the LUCPlanFLN20X, 40X, 60X, etc. are provided with a correction collar @. The correction is possible according to the vessel thickness. 1. If the thickness of the vessel bottom is known: Match the correction collar to the thickness of the vessel bottom using the collar scale ² provided. 2. If the thickness of the vessel bottom is unknown: The optimum position for the correction collar can be obtained from the image resolution. If a satisfactory sharp image is not obtained after focus adjustment, rotate the correction collar to the left and right so that you can compare the images at both sides. Reset the collar to the better image, then starting from this position, further rotate the collar to the left and right until both images can be obtained for comparison. By repeating this procedure several times, you will find best position for the correction collar. Refocus after rotating the correction collar.

CKX41/CKX31

PHASE CONTRAST OBSERVATION The following two slider units are available for phase contrast observation. Mount a slider onto the microscope and replace the objectives with phase contrast compatible objectives. Model Name

Description

Applicable Objectives (Note)

Ph precentering slider IX2-SLP

· The light annuli are precentered, so no adjustment is required. (For 4X, for 10X/20X/40X, and empty position) · The empty position can be used as a filter holder.

UPlanFLN4XPhP CAchN10XPhP LCAchN20XPhP LCAchN40XPhP

Ph centering slider IX2-SL

· The light annulus has to be centered. (For PHL, for PHC/PH1, and for PH2/empty) · The empty position can be used as a filter holder.

UPlanFLN4XPh (PHL) CPlanN10XPh (PHC) LCAchN20XPh (PHC) PlanN10XPh (PH1) LUCPlanFLN 20XPh (PH1) LCAchN40XPh (PH2)

(Note) Only the four objectives listed can be used with the IX2-SLP. If other objective is used, the phase contrast effect cannot be obtained.

1

Names of Parts Phase Sliders Ph precentering slider IX2-SLP For 4X (fixed)

Empty For 10X, 20X or 40X (fixed)

Ph centering slider IX2-SL

Optical element IX2-SLPHC IX2-SLPH1

Optical element IX2-SLPH2

For PHL (built in)

Centering knobs # The IX2-SLPH2 does not need centering.

17

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³

2

²

Fig. 21

3 @ ²

Fig. 22

18

(Fig. 21)

}When the IX2-SL phase contrast slider is used, it is required to attach an optical element matching the phase objective in use. 1. Hold the IX2-SLPH2 optical element 1 so that engraving faces upward and drop in the optical element in the empty position 2. 2. When mounting the IX2-SLPHC or IX2-SLPH1 optical element 3, insert the provided centering knobs into the screw holes 5, loosen them fully before dropping the optical element in the empty position 4 so that the engraving faces upward. 3. After dropping in the optical element, lightly tighten the centering knobs.

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Mounting the Optical Element

Mounting the Phase Slider

(Fig. 22)

1. Hold the phase slider 1 face up (engraving side up) with the finger hold on the right, and insert it into the illumination column slot. 2. When performing phase contrast observation, always set the aperture iris diaphragm lever 2 to “(” (wide open).