PerfectSpin 24 R Instruction Manual Ver 1

CONTENTS 1

SAFETY PRECAUTIONS

1

2

GENERAL DESCRIPTION

2

2.1 2.2 2.3 3

Description Technical specifications Warranty

INSTALLATION 3.1 3.2 3.3 3.4 3.5 3.6 3.7

Unpacking the centrifuge Installation Opening and Closing the Lid Loading the Rotor Overloading rotors Starting and Stopping a Run Emergency Lid Release

2 2 3 3 3 3 4 4 4 5 5

4

OVERVIEW

6

5

OPERATION

7

5.1 5.2 5.3 5.4 5.5 5.6 5.7 6

Setting the Time (TIME) Setting the Speed (RPM) Setting the Centrifugal Force (RCF) Setting the Temperature (TEMP) Cooling Quick-Spin Operation Changing the Settings During a Run

SERVICE AND MAINTENANCE 6.1 6.2 6.3 6.4 6.5 6.6

7 7 7 8 8 8 8 9

Centrifuge service Cleaning the centrifuge Cleaning the rotor Disinfection Replacing the Rotor Replacing fuses

9 9 9 9 9 9

7

TROUBLESHOOTING GUIDE

10

8

TECHNICAL SERVICE AND ORDERING INFORMATION

11

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Instruction Manual PerfectSpin 24 R Digital, refrigerated Microcentrifuge

1 SAFETY PRECAUTIONS Before using the PerfectSpin 24 R refrigerated microcentrifuge for the first time, please read this entire operating manual carefully. To guarantee problem free, safe operation, it is essential to observe the following points: 1. When using infectious, radioactive, toxic and other solutions which may pose health risks, please observe the appropriate safety precautions. 2. Do not use this machine in a potentially explosive environment or with potentially explosive chemicals. 3. Install the machine in a location free of excessive dust. 4. Avoid placing the machine in direct sunlight. 5. Choose a flat, stable surface capable of withstanding the weight of the machine. 6. Install the machine at room temperature 15-30 °C, relative humidity 20-80 %. 7. Don’t block the air vents. 8. Make sure the power source conforms to the required power supply specifications. 9. To avoid electric shock, make sure the machine is plugged into a grounded electric outlet. 10. Do not allow water or any foreign objects to enter the various openings of the machine. 11. Switch off the power switch before cleaning or performing any service on the machine, such as replacing the fuses. 12. The rotor and the rotor lid must always be installed securely on the motor shaft. 13. Always load the rotor symmetrically. Each tube should be counterbalanced by another tube of the same type and weight. 14. To guarantee sufficient ventilation, ensure that the centrifuge has at least 30 cm of free space on all sides, including the rear. 15. Repairs should be carried out by PEQLAB authorized service personnel only. 16. Use original spare parts and accessories only. Please be aware of the danger of electric shock or other dangers.

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Instruction Manual PerfectSpin 24 R Digital, refrigerated Microcentrifuge

2 GENERAL DESCRIPTION 2.1 Description The PerfectSpin 24 R refrigerated microcentrifuge is a compact, bench-top centrifuge which is suitable for both research and clinical laboratories. The motor is brushless and requires no routine maintenance. It is supplied with a 24 x 1.5 / 2.0 ml rotor for micro samples. The maximum speed of the PerfectSpin 24 R is 13,500 rpm (17,135 rcf). The temperature range is from –10 °C to 40 °C. This manual provides important safety information for the PerfectSpin 24 R refrigerated microcentrifuge (Cat. No. 91-PSPIN-24R). It should be kept near the centrifuge for quick and easy reference. -

Brushless motor drive, control speed up to 13,500 rpm/17,135 x g Powerful refrigeration system, maintain 4 °C at max. speed Includes unique easy access rotor (24 x 1.5 ml) Exceptionally quiet and compact Optional StripSpin adapter available for 0.2 ml tubes and strips Compact, ergonomic design User-friendly operation interface Quick button for instant run without setting Low noise level Complies with CE, RoHS

2.2 Technical specifications Model Max. Speed Max. RCF Acceleration Time Deceleration Time Temperature Range Max. Rotor Capacity Rotor Lid Time Range Admissible Sample Density Noise at Max. Speed Operating Temperature Operating Humidity (RH) Power Supply

91-PSPIN-24R 13,500 rpm 17,135 g < 17 seconds < 18 seconds –10 °C to 40 °C 24 x 2.0 / 1.5 ml tubes Easy snap-on type 0.5 to 99 min. or “continuous” 1.2 g / ml 56 dB 5 °C to 30 °C 20 - 80 % 100 V / 50~60 Hz, 6.0 A 110 V / 50~60 Hz, 5.5 A 230 V / 50~60 Hz, 2.8 A 280 x 430 x 248 mm 22 kg Complies with CE, Class B (EN60101-1-2, EN 55011) Complies with RoHS

Dimensions (W x D x H) Weight (with Rotor) Certification

Note: Specifications are subject to change without prior notice. PEQLAB Biotechnologie GmbH_v1208E

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Instruction Manual PerfectSpin 24 R Digital, refrigerated Microcentrifuge

2.3 Warranty PEQLAB guarantees that the PerfectSpin 24 R refrigerated microcentrifuge you have received has been thoroughly tested and meets its published specification. However, immediately upon arrival, please check carefully that the shipment is complete and has not been damaged in transit. For missing parts or to report any kind of damage, please contact PEQLAB (see 'TECHNICAL SERVICE’). Please retain all packaging materials until the delivery has been completely checked since this will speed up the return of goods if required and reduce environmental impact. Any form of returns, replacements or credit notes must be agreed in advance by PEQLAB. This centrifuge has been subjected to thorough testing and quality control. In the unlikely event of a manufacturing fault, our two year warranty (from the date of delivery) covers the centrifuge and the rotor. This warranty becomes invalid in the case of incorrect operation, use of non-standard spare parts or accessories and unauthorized modification of the rotor or centrifuge. PEQLAB reserves the right to make technical modifications.

3 INSTALLATION 3.1 Unpacking the centrifuge The centrifuge is delivered in an external carton and an internal carton with protective PE foam cushions. Remove the centrifuge from each carton. All packaging should be retained until it has been established that the centrifuge is working properly. Inspect the centrifuge for any visible signs of shipping damage. Shipping damage is the responsibility of the transport company. Any claims for damage must be filed within 48 hours with the company that delivered the centrifuge. Confirm that all items are included in the PerfectSpin 24 R refrigerated microcentrifuge package: -

PerfectSpin 24 R unit with a 24 x 1.5 / 2 ml rotor and a rotor lid

-

Operation manual

-

Electric fuses (7A & 3.15A)

-

Warranty card

-

Rotor wrench

-

Power cord

The accessories supplied with the centrifuge should be kept with the instruction manual near the centrifuge’s place of installation. If there are any items missing, damaged, or not according to your order, please contact your distributor or sales representative immediately.

Note: Please use 7A fuse in power source 100~120/50~60Hz countries, while 3.15A fuse is for power source 200~230V/50~60Hz countries.

3.2 Installation Place the machine on a stable, flat table. Please keep the machine at least 30 cm from the surrounding area or objects.

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Instruction Manual PerfectSpin 24 R Digital, refrigerated Microcentrifuge

Mains power and frequency “must must” must be compatible with the information given on the product label on the rear of the centrifuge. Connect the power cord to the power socket of the centrifuge, then to the power source. Switch the Power On/Off switch to turn on the centrifuge. After the LCD display is on and the buzzer beeps twice, the centrifuge is ready to operate.

3.3 Opening and Closing the Lid The lid can be opened only when the centrifuge is powered on. Press

button to open the lid.

The lid will be locked automatically when the user presses down the front edge of the lid.

Note: Do not slam the lid!

3.4 Loading the Rotor For safety reasons, the rotor must be loaded with centrifuge tubes symmetrically. The difference in the weight between the tubes should not exceed 0.1 g. The centrifuge tubes should be loaded in pairs opposite each other and each should contain roughly the equal weight of sample. One or two additional loaded tubes may need to be added to achieve this. Please refer Figure 1 to see an example of a properly loaded rotor. Attention: To reduce air friction and noise, the rotor lid should be attached to the rotor during centrifugation. Make sure that the rotor lid snaps snaps securely into place, by pressing down on center catch.

Securing screw

Figure 1: Loading the rotor

3.5 Overloading rotors The maximum load of the rotor and the maximum speed have been established by the manufacturer. Do not attempt to exceed these values. The maximum speed of the rotor has been established for liquids having a homogeneous density of 1.2 g / ml or less. In order to centrifuge liquids with a higher density it is necessary to reduce the speed. Failure to reduce the speed may result in damage to the rotor and centrifuge. The revised maximum speed can be calculated with the following formula: Reduced speed (nred) =

1.2 x max speed (nmax) higher _ density _ value

Example: Where the density of the liquid is 1.7, the new maximum speed would be calculated as follows:

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Instruction Manual PerfectSpin 24 R Digital, refrigerated Microcentrifuge

nred =

1.2 x 13,300 rpm = 11,174 rpm 1.7

If in doubt concerning maximum speeds, please contact the manufacturer for assistance.

3.6 Starting and Stopping a Run Please refer Chapter 5 Operation to set up your preferred settings of time, speed and temperature. To start a run, press down the

(START/STOP START/STOP) START/STOP knob.

When the pre-set time expires, the centrifuge will stop automatically. To stop the centrifuge prior to the expiration of set time, press the (START/STOP START/STOP) START/STOP knob again. When the time is set as “continuous continuous”( continuous - - ), the centrifuge will run continuously. To stop the continuous run, press the (START/STOP START/STOP) START/STOP knob. By pressing and holding the run will stop if the

button, the centrifuge will continue a short-run of set speed. The short-

button is released.

Note: For safety reasons, the (START/STOP START/STOP) START/STOP knob will be inactivated when the lid is open. A symbol ” will be flashing until the lid is closed. “

3.7 Emergency Lid Release When the lid can’t be opened by pressing the button, users can open the lid manually. First, turn off the power of the centrifuge. Second, use a flat-head screw driver or other tool to remove the Emergency Lid Release Plug (Please refer Figure 2) on the right-hand side of the machine. Third, pull the wire (attached to the plug) while gently pushing downward on the lid. The lid will now release.

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Instruction Manual PerfectSpin 24 R Digital, refrigerated Microcentrifuge

4 OVERVIEW This section presents an overview of the PerfectSpin 24 R microcentrifuge’s various components and control panel (see Figure 2) as well as the symbols and indicators on the LCD display (see Figure 3). Lid lock hole Air vents

Power On/Off switch & fuse box Emergency lid release plug

Figure 2: PerfectSpin 24 R refrigerated microcentrifuge overview

Figure 3: LCD display panel Keypad Function Button START/STOP and Value Adjustment Knob

Symbol

TIME button

Function Description - Start/Stop a run by pressing down - Rotate to Increase/decrease the values of time, temperature, speed or g force - To adjust run time setting

RPM button

-

To adjust speed setting

RCF button

-

To adjust RCF setting

TEMP button

-

To adjust temperature setting

LID button

-

Open the lid

QUICK button

-

Quick-Spin centrifugation

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Instruction Manual PerfectSpin 24 R Digital, refrigerated Microcentrifuge

Audible Signals The audible signals are beneficial when you are familiarizing yourself with the operating procedure of the PerfectSpin 24 R refrigerated microcentrifuge. They can also be disabled if desired by pressing button six times. The audible signal function can be resumed by pressing button six times again. Definition of the audible sounds are shown in the table below. Audible Sound 2 beeps 3 beeps

Operation Power on machine End of a run

5 OPERATION Users can pre-set the time, temperature and speed (RPM/RCF) before a run.

Attention: Never attempt to operate the centrifuge with rotors or adapters adapters that show signs of corrosion or mechanical damage. Never centrifuge strongly corrosive materials that may damage the rotors, accessories or bowl of the unit.

5.1 Setting the Time (TIME) Operating time can be selected from 0.5 min to 99 min or “continuous”. Press button to change the time setting. The 2 digits of TIME on the display will flash. Turn the (START/STOP START/STOP) START/STOP knob to increase or decrease the value. Press button again to confirm the selection or press (START/STOP START/STOP) START/STOP knob to store and run the selection. The flashing of time value will stop and the time value will be stored automatically if no button is pressed after 6 seconds of the adjustment. (START/STOP START/STOP) knob clockwise or counterOperating time can be set as “continuous continuous” START/STOP continuous by turning the clockwise to make the 2 digits of TIME to display “-- -“. When the time is set as “continuous”, the centrifuge will continue to run without a stop. Press (START/STOP START/STOP) START/STOP knob to end the continuous operation.

5.2 Setting the Speed (RPM) The speed (RPM) can be selected from 500 to 13,500 rpm. Press button to change the speed setting. The 5 digits of speed (RPM) on the display will flash. Turn the (START/STOP START/STOP) START/STOP knob to increase or decrease the value. Press button again to confirm the selection or press (START/STOP START/STOP) START/STOP knob to store and run the selection. The flashing of RPM value will stop and the RPM value will be stored automatically if no button is pressed after 6 seconds of the adjustment.

5.3 Setting the Centrifugal Force (RCF) The speed can be selected from 20 to 17,200 x g. Press button to change the RCF setting. The 5 digits of RCF on the display will flash. Turn the (START/STOP START/STOP) START/STOP knob to increase or decrease the value. Press button again to confirm the selection or press (START/STOP START/STOP) START/STOP knob to store and run the selection. The flashing of RCF value will stop and the RCF value will be stored automatically if no button is pressed after 6 seconds of the adjustment.

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Instruction Manual PerfectSpin 24 R Digital, refrigerated Microcentrifuge

5.4 Setting the Temperature (TEMP) The temperature can be selected from -10 to 40 °C. Press button to change the temperature setting. (START/STOP START/STOP) The 2 digits of temperature on the display will flash. Turn the START/STOP knob to increase or decrease the value. Press button again to confirm the selection or press (START/STOP START/STOP) START/STOP knob to store and run the selection. The flashing of TEMP value will stop and the value will be stored automatically if no button is pressed after 6 seconds of the adjustment.

5.5 Cooling The temperature can be set from -10 °C and 40 °C. The set temperature can also be changed during the run. Pre Pre-Cooling Pre-Cooling starts a temperature-control run at 6,000 rpm. It allows the rotor to be cooled down to the new, pre-set temperature quickly. Cooling down from room temperature to 4 °C takes approximately 8 min. (max. 16 minutes). To activate Pre-Cooling function, press and hold button, then press (START/STOP START/STOP) START/STOP key. Both “RPM” and “TEMP” icons will flash during Pre-Cooling. The Pre-Cooling can be stopped by pressing the (START/STOP START/STOP) START/STOP key. The Pre-Cooling will function only when the set temperature is lower than the ambient temperature. Standby Cooling When the centrifuge is powered on, the refrigeration system will cool down the chamber to the pre-set temperature before or after the run if the chamber temperature is above the pre-set temperature. Note:

1. To extend the life cycle of the compressor, always remember to inactivate the Standby Cooling (by increasing the set temperature above room temperature) or power off the centrifuge when it is not in use. 2. When the lid is open, the Standby Cooling will stop after 3 minutes.

5.6 Quick-Spin Operation The centrifuge can be operated for a short run by pressing and holding the

button. The centrifuge

will continue to run as long as the button is depressed and the time, in seconds, will count up on the time display. The Quick-Spin speed and temperature is based on the last run settings remaining in the memory. The icon “RPM RPM” RPM will flash during Quick-Spin operation.

5.7 Changing the Settings During a Run Users can change the settings while the rotor is running. By pressing any function button once ( , , , ), the current value will switch into the pre-set value mode. The to-be-changed value flashes, and can then be adapted. Once the display switches into the current value mode after completion of the entered values, the new values are activated. The new values are stored and executed by pressing the function button again or by waiting 6 seconds.

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Instruction Manual PerfectSpin 24 R Digital, refrigerated Microcentrifuge

6 SERVICE AND MAINTENANCE Always unplug the power plug before service and maintenance.

6.1 Centrifuge service The brushless motor in the PerfectSpin 24 R requires no routine maintenance. Any required service should be performed by authorized, qualified personnel only. Repairs performed by unauthorized personnel voids the warranty.

6.2 Cleaning the centrifuge Always keep the centrifuge housing, rotor chamber, rotor and rotor accessories clean. All parts should be wiped down periodically with a soft cloth. For more thorough cleaning, use a neutral cleaning agent (pH between 6 and 8) applied with a soft cloth. Excessive amounts of liquid should be avoided. Liquid should not come into contact with the motor. After cleaning, ensure that all parts are dried thoroughly by hand or in a warm air cabinet (maximum temperature 50 °C).

6.3 Cleaning the rotor The rotor should be cleaned after each use. When spinning samples containing phenol or phenol chloroform, the rotor should be cleaned immediately after use.

6.4 Disinfection Should a spill of infectious materials occur within the rotor or chamber, the unit should be disinfected. This should be performed by qualified personnel with proper protective equipment.

6.5 Replacing the Rotor The PerfectSpin 24 R comes complete with a standard 24-place rotor installed. To remove the rotor, remove the rotor securing screw from the motor shaft by turning the screw, using the rotor wrench on two holes of the securing screw (please refer figure 1). Lift the rotor directly upward in a straight vertical motion. To replace rotor, first make sure the motor shaft and rotor mounting hole are clean. Place the rotor on the motor shaft. Reinstall the rotor securing screw on the motor shaft by turning it clockwise. Hold the rotor with one hand and tighten the rotor securing screw, using the rotor wrench.

6.6 Replacing fuses Check the fuse when it is recommended in the Troubleshooting Guide located in this manual. The fuse holder is located in the power inlet on the rear of the unit. Disconnect the power cord from the power inlet. Open the fuse holder drawer by inserting a small screwdriver under the tab and prying it open. Remove the innermost (operative) fuse from its retaining tabs and replace the fuse if necessary. A spare fuse is located in the outermost chamber of the fuse drawer. Replace only with a fuse of exactly the same value as the original.

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Instruction Manual PerfectSpin 24 R Digital, refrigerated Microcentrifuge

7 TROUBLESHOOTING GUIDE To ensure the product’s quality and performance, the PerfectSpin 24 R refrigerated microcentrifuge is 100 % inspected by the manufacturer. Error messages will appear on the display if the PerfectSpin 24 R refrigerated microcentrifuge fails to perform the attempted action properly. In the case of Error (Err) messages or faults, please refer to the solutions in the following table to clear the error messages or faults. If the following solutions are not able to resolve the problem, please contact PEQLAB’s Customer Service Department at +49 91 31 / 6 10 70 20 to arrange for authorized service. Symptom

Possible Cause

Solution

“

Press (START/STOP START/STOP) START/STOP knob when Lid is open.

Close the lid

Defective lid lock sensor

Call for service

Tubes are not inserted symmetrically in rotor holes

Load tubes symmetrically

Sample liquid in tubes not properly balanced

Make sure that the same volume of liquid is in each tube

Defective or improperly adjusted balance sensor

Call for service

Rotor is stuck when a run starts

Call for service

Lid not closed correctly

Close lid correctly

No speed or time has been selected

Set speed and/or time

No power supply

-

“

“ Error

” Error (rotor imbalance)

Centrifuge cannot be started, although power is on Centrifuge will not start

Blown fuse Lid Lock will not release

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Check power source Check the power cord connection - Check the power cord quality Check the fuse and replace if necessary

Defective lid lock

Open manually and have unit serviced

No power from PC board

Call for service

Lid lock jammed

Call for service

Centrifuge is not receiving power

See “Centrifuge will not start”

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Instruction Manual PerfectSpin 24 R Digital, refrigerated Microcentrifuge

8 TECHNICAL SERVICE AND ORDERING INFORMATION Should you have any questions about the PerfectSpin 24 R or its accessories, please call PEQLAB’s Customer Service Department at +49 91 31 / 6 10 70 20. Please have the unit’s serial number (located on the back panel of the instrument) available when calling. Should an item require return to PEQLAB for service, a decontamination form must be completed first by the user. Items sent without decontamination form will not be accepted. All returns must be pre-approved by PEQLAB! The following accessories are included or available for the PerfectSpin 24 R: Angle rotor for 24 x 1.5 ml tubes Tube measurement Max. speed Centrifuging radius Max. RCF (g-value)

(Included) 1.5 ml / 2.0 ml (10 x 40 mm) 13,500 rpm 8.4 cm 17,135 x g

Rotor Removal Tool Order no.

(Included) 91-PSPIN-RW

Rotor Lid Order no.

(Included) 91-PSPIN-RL

Rotor Securing Screw Order no.

(Included) 91-PSPIN-RS

Strip Spin Adapter Order no. Tubes accepted Max Speed Range Centrifuge radius Range RCF (g value)

91-PSPIN-AS 0.2 ml tubes or 8 x 0.2 ml strips 13,500 rpm 4.32 cm to 5.33 cm 8,802 x g to 10,860 x g

Adapter for 0.5 ml tubes (pk./6) Order no. Tube measurement Max. speed Centrifuging radius RCF (g-value)

91-PSPIN-A05 8 x 30 mm 13,500 rpm 7.53 cm 15,342 x g

Adapter for 0.2 ml tubes (pk./6) Order no. Tube measurement Max. speed Centrifuging radius RCF (g-value)

91-PSPIN-A02 6 x 21 mm 13,500 rpm 7.03 cm 14,324 x g

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Instruction Manual PerfectSpin 24 R Digital, refrigerated Microcentrifuge

NOTES

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Instruction Manual PerfectSpin 24 R Digital, refrigerated Microcentrifuge

NOTES

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