COBE 2991 Cell Processor Overview 2012

COBE® 2991™ CELL PROCESSOR OVERVIEW

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COBE® 2991 Cell Processor

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Presentation Overview

 Presentation Objectives  Operational Principles  Operator Controls  LED Program Board Overview  Using the LED Program Board

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COBE® 2991 Cell Processor

Presentation Objectives

Participants will be able to:  List the three operations that constitute a wash cycle  State the three LED program board modes  Explain STOP SELECT and define CALL, R.C. and P.C.  Describe how to program a Hold at the start of the Agitate Wash-In operation

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COBE® 2991 Cell Processor

COBE 2991 Cell Processor

WPC DFRBC Research Applications

Bone Marrow Processing 6

COBE® 2991 Cell Processor

COBE 2991 CELL PROCESSOR Operational Principles

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COBE® 2991 Cell Processor

Centrifugal Separation Technology

 Separation of product components based on specific gravities  Removal and/or collection of desired fraction  Addition and agitation of wash solutions to remove unwanted cells, debris and fluids

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COBE® 2991 Cell Processor

COBE 2991 Overview

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COBE® 2991 Cell Processor

Basic Process Three operations per cycle:  Start/Spin  Supernatant-Out  Agitate/Wash-In

Spin time, centrifuge speed, super out rate and volume; minimum agitate time, valve selections and number of cycles can be programmed according to different protocols. Stop options:  Call  Packed cells  Resuspended cells

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COBE® 2991 Cell Processor

Start/Spin

 Centrifuge spins at set speed  RBCs and Buffy Coat sediment based on their specific gravities  Plasma collects in the center

RBCs Buffy Coat Plasma

RBC wash example 11

COBE® 2991 Cell Processor

Supernatant-Out

 Centrifuge continues to spin  Supernatant out valve opens  Flexible diaphragm inflates Flexible diaphragm Hydraulic pump

RBC wash example 12

COBE® 2991 Cell Processor

 Supernatant collects in waste collection bag

Supernatant-Out RBC Detect

Supernatant-Out operation continues until:  RBCs are detected by the red cell detector (RCD) RCD

-or-

 Desired supernatant volume is reached

RBC wash example 13

COBE® 2991 Cell Processor

Agitate/Wash-In

Wash solution

 Centrifuge slows and begins to agitate  Selected wash valve opens  Flexible diaphragm deflates  Wash solution and RBCs mix

RBC wash example 14

COBE® 2991 Cell Processor

Validated Protocols

 1-L Wash Packed or Resuspended RBCs  2-L Wash Packed or Resuspended RBCs  Deglycerolized High Glycerol Frozen RBCs with or without resuspension  Deglycerolized High Glycerol Frozen RBCs Low-Volume Unit (60 mL to 80 mL)  Deglycerolized Low-Glycerol Frozen RBCs

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COBE® 2991 Cell Processor

Validated Protocols (continued)

 Single Unit Leukocyte Concentrates  Collection of Young RBCs  Bone Marrow Processing Using a Density Gradient Separation Medium  Bone Marrow Concentration  Mononuclear Cell Washing  Cell Concentration  Large Volume Wash

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COBE® 2991 Cell Processor

OPERATOR CONTROLS

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COBE® 2991 Cell Processor

Main Control Panel

Program board

Operator controls

Valve assembly RCD

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COBE® 2991 Cell Processor

 LED program board  Rotary dials/controls  Auto/manual button  Lighted operator control buttons and status lights

Secondary Control Panel

 Pump restore rate  Red cell override timer (RCO)  Circuit breakers  Audible alarm (primary)

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COBE® 2991 Cell Processor

LED PROGRAM BOARD OVERVIEW

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COBE® 2991 Cell Processor