CO2 Incubators Models CB 53, CB 150 and CB 210 Operating Manual Dec 2012

Product registration

CB (E3) 12/2012

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Contents EC – declaration of conformity ... 2 Product registration ... 5

1.

SAFETY ... 9

1.1 1.2

Legal considerations ... 9 Structure of the safety instructions ... 9 1.2.1 Signal word panel ... 9 1.2.2 Safety alert symbol ... 10 1.2.3 Pictograms ... 10 1.2.4 Word message panel structure ... 11 1.3 Localization / position of safety labels on the unit ... 11 1.4 Type plate... 12 1.5 General safety instructions on installing and operating the CO2 incubator... 13 1.6 Precautions when working with gases ... 14 1.7 Precautions when handling gas cylinders ... 16 1.8 Intended use ... 16

2.

UNIT DESCRIPTION ... 17

2.1 2.2 2.3

Unit overview ... 18 Instrument panel for CB ... 19 Control panel at the rear of the unit ... 20

3.

COMPLETENESS OF DELIVERY, TRANSPORTATION, STORAGE, AND INSTALLATION... 21

3.1 3.2 3.3 3.4

Unpacking, and checking equipment and completeness of delivery ... 21 Guidelines for safe lifting and transportation... 22 Storage ... 22 Location of installation and ambient conditions ... 22

4.

INSTALLATION AND CONNECTIONS ... 26

4.1

CO2 and O2 sensors ... 26 4.1.1 The CO2 measuring principle ... 26 4.1.2 General notes ... 26 4.1.3 Connecting the CO2 sensor ... 27 4.1.4 Connecting the O2 sensor (unit with O2 control) ... 27 4.2 Shelves... 27 4.3 Permadry™ water pan ... 28 4.4 Gas connections ... 29 4.4.1 Connection of the CO2 gas cylinder ... 29 4.4.2 Connection of the O2 gas cylinder (unit with O2 control)... 31 4.4.3 Connection of the N2 gas cylinder (unit with O2 control) ... 32 4.4.4 Connecting the gas hose to the unit rear (for CO2, O2, N2) ... 33 4.4.5 Gas cylinder connection kits (option) ... 35 4.4.6 Connection of the CO2 gas cylinder with Fail Safe (option) ... 36 4.4.7 Connecting the built-in gas cylinder changer for CO2 (option for CB 150 / CB 210) ... 37 4.4.8 Connecting the built-in CO2 gas cylinder changer with an external gas connection for a second incubator (option for CB 150 / CB 210)... 37 4.4.9 Connection of the built-in gas cylinder changers for N2 and O2 (option for CB 150 / CB 210 with O2 control) ... 38 4.5 Electrical connection ... 39 4.6 Handling and aligning the multiple-divided inner glass door, gas proof (optional equipment) ... 40

5.

START UP ... 41

5.1 5.2 5.3

Function overview of the MB1 display controller... 41 Factory settings ... 42 Turning on the unit ... 42

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6.

CONTROLLER MB1 SETTINGS... 43

6.1 6.2 6.3 6.4 6.5 6.6

Selection of the menu language ... 43 Overview of controller MB1 displays ... 44 Menu settings in the “User-settings” menu ... 45 Menu settings in the “User Level” menu ... 46 Sea level data entry ... 47 Graphic representation of the historical measurement (chart recorder function) ... 48 6.6.1 Setting the storage rate... 50 6.7 Entering the set point values ... 51 6.7.1 Setting ranges ... 51 6.7.2 Note when setting high gas concentrations ... 52 6.8 Deactivating the O2 control (unit with O2 control) ... 53 6.9 Humidity control of the Permadry™ system... 54 6.10 Performance during and after power failures and shut down ... 54

7.

TEMPERATURE SAFETY DEVICES ... 55

7.1 7.2

Over temperature protective device (class 1) ... 55 Safety controller (temperature safety device class 3.1) ... 55

8.

NOTIFICATION AND ALARM FUNCTIONS ... 57

8.1 8.2 8.3 8.4 8.5 8.6 8.7 8.8

Notification and alarm system overview (auto diagnosis system) ... 57 Resetting the notification or alarm messages ... 58 Zero-voltage relay alarm output ... 59 Door open... 60 High and low temperature ... 60 CO2 over / under concentration ... 61 O2 over / under concentration (unit with O2 control) ... 61 Low pressure indication in case of empty gas cylinders ... 62

9.

OPTIONS ... 63

9.1

Silicone access ports 30 mm / 1.18 in, closable with 2 silicone plugs (8012-0558 rear, 8012-0559 left, 8012-0560 right) (option) ... 63 9.2 Interior socket 230V (option for CB 150 / CB 210) ... 64 9.3 Analog outputs for temperature and CO2 (option) ... 65 9.4 Access port for extra-low voltage (option for CB 150 / CB 210) ... 66 9.5 Keyboard locking (option) ... 67 9.6 Communication software APT-COM™ 3 DataControlSystem (option) ... 67 9.7 Ethernet interface (option)... 67 9.8 CO2 Fail Safe (option) ... 68 9.8.1 Function overview ... 68 9.8.2 Fail Safe alarm ... 68 9.8.3 Error detection in case of Fail Safe alarms ... 69 9.8.4 Detailed information of the time history regarding learning and comparison ... 69 9.9 Built-in gas cylinder changers (option) ... 70 9.9.1 Built-in gas cylinder changer for CO2 (option for CB 150 and CB 210) ... 70 9.9.2 Built-in cylinder CO2 changer with external gas connection for a second incubator (option for CB 150 and CB 210) ... 70 9.9.3 Built-in gas cylinder changers for N2 and O2 (option for CB 150 and CB 210 with O2 control)71 9.9.4 External gas cylinder changer for CO2, N2 or O2 (option) ... 72 9.10 Stands ... 72 9.10.1 Stacking stand (option) ... 72 9.10.2 Stacking adapter for direct thermal decoupled stacking (option) ... 72 9.10.3 Base on castors (option) ... 73

10. REFERENCE MEASUREMENTS ... 73 10.1 CO2 reference measuring ... 73 10.1.1 Measuring the CO2 concentration indirectly via the pH of the cell medium ... 73 10.1.2 Measuring CO2 directly via chemical indicator tubes... 74 10.1.3 Measuring CO2 directly with an electronic infrared measuring device ... 75 10.2 Temperature reference measurement ... 75

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11. AVOIDING MICROBIAL CONTAMINATION... 76 11.1 11.2 11.3 11.4 11.5

Cells and media ... 76 Laboratory conditions / equipment around the incubator ... 76 Working and behavior in the lab ... 76 Chamber design and equipment of the CO2 incubator ... 77 Handling the CO2 incubator ... 78

12. CLEANING, DECONTAMINATION / DISINFECTION, AND STERILIZATION ... 79 12.1 Cleaning ... 79 12.2 Decontamination / chemical disinfection of the CO2 incubator ... 80 12.3 Disinfection of the CO2 sensor ... 81 12.4 Sterilization of the O2 sensor (unit with O2 control) ... 82 12.5 Hot-air sterilization at 180 °C / 356 °F... 82 12.5.1 Overview ... 82 12.5.2 Procedure for hot-air sterilization ... 83 12.5.3 Terminating the sterilization cycle ... 85

13. MAINTENANCE AND SERVICE ... 86 13.1 13.2 13.3 13.4 13.5

Maintenance intervals, service ... 86 Checking the air jacket heating fan ... 87 Checking the humidity system fan ... 87 Gas inlet fine filter ... 87 Sending the unit back to BINDER GmbH ... 88

14. DISPOSAL... 89 14.1 Disposal of the transport packing ... 89 14.1.1 Outer unit packing ... 89 14.1.2 Packing inside the unit and equipment ... 89 14.2 Decommissioning ... 90 14.3 Disposal of the unit in the Federal Republic of Germany ... 90 14.4 Disposal of the unit in the member states of the EC except for the Federal Republic of Germany . 91 14.5 Disposal of the unit in non-member states of the EC ... 92

15. TROUBLESHOOTING ... 93 16. TECHNICAL DESCRIPTION ... 97 16.1 Factory calibration and adjustment ... 97 16.2 Over current protection ... 97 16.3 Definition of usable volume ... 97 16.4 CB technical data ... 98 16.5 CB equipment and options ... 100 16.6 Spare parts ... 101 16.7 Important conversion data for non-SI units ... 102 16.8 Conversion table for gas inlet pressures, bar – psi ... 102 16.9 Dimensions CB 53 ... 103 16.10 Dimensions CB 150 ... 104 16.11 Dimensions CB 210 ... 105

17. CONTAMINATION CLEARANCE CERTIFICATE ... 106 17.1 For units located outside North America and Central America ... 106 17.2 For units in North America and Central America ... 109

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Dear customer, For the correct operation of the CO2 incubator CB, it is important that you read this operating manual completely and carefully and observe all instructions as indicated. Failure to read, understand and follow the instructions may result in personal injury. It can also lead to damage to the unit and/or poor equipment performance.

1.

Safety

This operating manual is part of the components of delivery. Always keep it handy for reference. The device should only be operated by laboratory personnel especially trained for this purpose and familiar with all precautionary measures required for working in a laboratory. To avoid injuries and damage observe the safety instructions of the operating manual.

WARNING Failure to observe the safety instructions. Serious injuries and unit damage.  Observe the safety instructions in this operating manual  Carefully read the complete operating instructions of the CO2 incubator CB.

1.1

Legal considerations

This operating manual is for informational purposes only. It contains information for installing, start-up, operation and maintenance of the product. Note: the contents and the product described are subject to change without notice. Understanding and observing the instructions in this operating manual are prerequisites for hazard-free use and safety during operation and maintenance. In no event shall BINDER be held liable for any damages, direct or incidental arising out of or related to the use of this manual. This operating manual cannot cover all conceivable applications. If you would like additional information, or if special problems arise that are not sufficiently addressed in this manual, please ask your dealer or contact us directly by phone at the number located on page one of this manual. Furthermore, we emphasize that the contents of this operating manual are not part of an earlier or existing agreement, description, or legal relationship, nor do they modify such a relationship. All obligations on the part of BINDER derive from the respective purchase contract, which also contains the entire and exclusively valid statement of warranty administration. The statements in this manual neither augment nor restrict the contractual warranty provisions.

1.2

Structure of the safety instructions

In this operating manual, the following safety definitions and symbols indicate dangerous situations following the harmonization of ISO 3864-2 and ANSI Z535.6.

1.2.1 Signal word panel Depending on the probability of serious consequences, potential dangers are identified with a signal word, the corresponding safety color, and if appropriate, the safety alert symbol.

DANGER Indicates an imminently hazardous situation that, if not avoided, will result in death or serious (irreversible) injury.

WARNING Indicates a potentially hazardous situation which, if not avoided, could result in death or serious (irreversible) injury CB (E3) 12/2012

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CAUTION Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor (reversible) injury

CAUTION Indicates a potentially hazardous situation, which, if not avoided, may result in damage to the product and/or its functions or of a property in its proximity.

1.2.2 Safety alert symbol Use of the safety alert symbol indicates a risk of injury. Observe all measures that are marked with the safety alert symbol in order to avoid death or injury.

1.2.3 Pictograms Warning signs

Electrical hazard

Hot surface

Explosive atmosphere

Stability hazard

Lifting hazard

Gas cylinders

Suffocation hazard

CO2 suffocation and poisoning hazard

Fire promoting agents

Harmful substances

Risk of corrosion and / or chemical burns

Lift with several persons

Explosive substances

Biohazard Mandatory action signs

Pollution Hazard

Mandatory regulation

Read operating instructions

Disconnect the power plug

Environment protection

Wear protective gloves

Wear safety goggles

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Prohibition signs

Do NOT touch

Do NOT spray with water

Do NOT climb

Information to be observed in order to ensure optimum function of the product.

1.2.4 Word message panel structure Type / cause of hazard. Possible consequences. ∅ Instruction how to avoid the hazard: prohibition  Instruction how to avoid the hazard: mandatory action Observe all other notes and information not necessarily emphasized in the same way, in order to avoid disruptions that could result in direct or indirect injury or property damage.

1.3

Localization / position of safety labels on the unit

The following labels are located on the unit: Pictograms (Warning signs)

Service label

Hot surface Risk of injury • on the outer door: CB-UL only • above the access ports (option)

CO2 incubator CB

CO2 incubator CB-UL

Figure 1: Position of labels on the CO2 incubator

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Keep safety labels complete and legible. Replace safety labels that are no longer legible. Contact BINDER Service for these replacements.

1.4

Type plate

Position of type plate: left unit side (seen from front), at the bottom in the middle Nominal temperature 187 °C 369°F Enclosure protection IP 20 Temp. safety device DIN 12880 Class 3.1 Art. No. 9040-0038 Project No.

1,40 kW 230 V 1 N ~ 6,1 A 50/60 Hz US PATS 4585923 / 5222612 / 5309981 5405194 / 5601143 / 5773287 / 6079403 D 78532 Tuttlingen / Germany Tel. + 49 (0) 7462/ 2005-0 Internet: www.binder-world.com

CB 150 Serial No. 00-00000 Made in Germany

Figure 2: Type plate (example of CB 150 regular unit) Indications of the type plate BINDER CB 150 Serial No. 00-00000 187 °C Nominal temperature 369°F Enclosure protection IP 20 Temp. safety device DIN 12880 Class 3.1 Art. No. 9040-0038 Project No. --1,40 kW 230 V 1 N ~ 6,1 A 50/60 Hz Symbol on the type plate

Information Manufacturer: BINDER GmbH Model CB 150 Serial No. 00-00000 Nominal temperature IP type of protection 20 acc. to EN 60529 Temperature safety device acc. to standard DIN 12880 Temperature safety device, class 3.1 Art. No. 9040-0038 (Special application acc. to project no.) Nominal power 1.40 kW Nominal voltage 230 V ± 10 %, single-phase unit Nominal current 6.1 Amp Power frequency 50/60 Hz

Information CE conformity marking Electrical and electronic equipment manufactured / placed on the market in the EC after 13 August 2005 and to be disposed of in separate collection according to directive 2002/96/EC on waste electrical and electronic equipment (WEEE). The equipment is certified in the GOST R certification system of GOSTSTANDARD Russia. ®

(CB-UL only) LABORATORY EQUIPMENT 43KM

CB (E3) 12/2012

The equipment is certified by Underwriters Laboratories Inc. accordnd ing to standards CAN/CSA-C22.2 No. 61010-1, 2 Edition, 2004-07 (Electrical Equipment for Measurement, Control, and Laboratory Use; nd Part 1: General Requirements); UL 61010-1, 2 Edition, 2005-07-22 (Electrical Equipment for Measurement, Control, and Laboratory Use; nd Part 1: General Requirements); IEC 61010-1:2001, 2 Edition and IEC 61010-2-10 (Particular Requirements for Laboratory Equipment for the heating of materials).

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1.5

General safety instructions on installing and operating the CO2 incubator

With regard to operating the CO2 incubator CB and to the installation location, please observe the guideline BGI/GUV-I on safe working in laboratories (formerly BGR/GUV-R 120 or ZH 1/119 laboratory guidelines issued by the employers’ liability insurance association) (for Germany). BINDER GmbH is only responsible for the safety features of the unit provided skilled electricians or qualified personnel authorized by BINDER perform all maintenance and repair, and if components relating to chamber safety are replaced in the event of failure with original spare parts. To operate the unit, use only original BINDER accessories or accessories from third-party suppliers authorized by BINDER. The user is responsible for any risk caused by using unauthorized accessories.

CAUTION Danger of overheating. Damage to the unit. ∅ Do NOT install the unit in unventilated recesses.  Ensure sufficient ventilation for dispersal of the heat. Do not operate the CO2 incubator CB in hazardous locations.

DANGER Explosion hazard. Danger of death. ∅ Do NOT operate the unit in potentially explosive areas.  KEEP explosive dust or air-solvent mixtures AWAY from the unit. The CO2 incubator CB does not dispose of any measures of explosion protection.

DANGER Explosion hazard. Danger of death.  Do NOT introduce any substance into the CO2 incubator which is combustible or explosive at working temperature. ∅ NO explosive dust or air-solvent mixture in the inner chamber. Any solvent contained in the charging material must not be explosive or inflammable. I.e., irrespective of the solvent concentration in the steam room, NO explosive mixture with air must form. The temperature inside the chamber must lie below the flash point or below the sublimation point of the charging material. Familiarize yourself with the physical and chemical properties of the charging material, as well as the contained moisture constituent and its behavior with the addition of heat energy and humidity. Familiarize yourself with any potential health risks caused by the charging material, the contained moisture constituent or by reaction products that may arise during the temperature process. Take adequate measures to exclude such risks prior to putting the CO2 incubator into operation.

DANGER Electrical hazard. Danger of death. ∅ The unit must NOT become wet during operation or maintenance.

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The CO2 incubators were produced in accordance with VDE regulations and were routinely tested in accordance to VDE 0411-1 (IEC 61010-1).

CAUTION The glass doors and the inner chamber will become hot during operation. Danger of burning. ∅ Do NOT touch the glass doors, the inner surfaces or the charging material during operation.

WARNING Stability hazard. Danger of injury. Damage to the unit and the charging material. Housing cover breakaway. ∅ Do NOT climb on the lower housing cover. ∅ Do NOT load the lower housing cover with heavy objects while the unit door is open.

1.6

Precautions when working with gases

Notes on handling carbon dioxide (CO2) Carbon dioxide (CO2) in high concentrations is hazardous to health. It is colorless and almost odorless and therefore practically imperceptible. Vent out any CO2 gas that may escape via good room ventilation or a suitable connection to an exhaust system. We recommend installing a CO2 warning system.

WARNING High concentration of CO2 (> 4 Vol.-%). Risk of death by suffocation. Danger of poisoning. ∅ Do NOT set up units in non-ventilated recesses  Ensure technical ventilation measures  Observe the relevant regulations for handling CO2. Unit with O2 control: Notes on handling oxygen (O2) Oxygen (O2) is colorless and almost odorless and therefore practically imperceptible. It promotes burns, which can proceed explosively. There is a fire hazard for flammable oxygenated materials, e.g. clothes and hair. O2 is heavier than air and may accumulate in low-lying areas.

WARNING High concentration of O2 (> 21 % O2). Fire and explosion hazard through contact of combustible materials with O2. Risk of burns and other injuries. ∅ Do NOT set up units in non-ventilated recesses  Ensure technical ventilation measures  Observe the relevant regulations for handling O2.

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Take appropriate measures to prevent oxygen enrichment and fire and explosion hazards in areas where oxygen enrichment is possible. General information for safe handling of oxygen: • Make sure training of personnel on hazards of oxygen enrichment and necessary safety measures • Make sure adequate labeling of all oxygen equipment and facilities • Make sure gas tightness of all gas connections by checking them for leaks (e.g. with leak spray or diluted soap solution) • Close the main valve of the source of oxygen after work when not using the chamber • Never lubricate O2 equipment with oil or fat. Use only materials and spare parts which are approved for use with oxygen. • Regularly inspect fire extinguishers for proper condition • Set up emergency showers where oxygen enrichment is possible • Strictest smoking ban and no ignition sources in areas where oxygen enrichment is possible • Make sure good ventilation of areas where oxygen enrichment is possible (location of the chamber and/or O2 cylinders • Persons who may have been in a possibly oxygen-enriched atmosphere must keep away from ignition sources (flames, cigarettes, etc.) and ventilate their clothes at least 15 minutes • Always keep emergency routes free Unit with O2 control: Notes on handling nitrogen (N2) Nitrogen (N2) in high concentrations is hazardous to health. It is colorless and almost odorless and therefore practically imperceptible. Any N2 gas that may escape must be safely led out via good room ventilation or a suitable connection to an exhaust system.

WARNING High concentration of N2. Risk of death by suffocation. ∅ Do NOT set up units in non-ventilated recesses  Ensure technical ventilation measures  Observe the relevant regulations for handling N2.

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1.7

Precautions when handling gas cylinders General information for safe handling of gas cylinders: • Store and use gas cylinders only in well-ventilated locations. • Open the gas cylinder valve slowly to avoid pressure surges • Secure gas cylinders during storage and use against falling (chaining). • Transport gas cylinders with a cylinder cart, do not carry, roll, or throw them. • Always close the valve even with apparently empty cylinders; screw on the cap when not in use. Return gas cylinders with the valve closed. • Do not open gas cylinders by force. Mark them when damaged • Protect gas cylinders against fire, e.g. do not store together with flammable liquids • Observe relevant regulations for dealing with gas cylinders.

Secure the gas cylinders against falling and other mechanical damage.

WARNING Safety valve tearing off. Sudden release of the stored pressure energy. Risk of injury.  Secure gas cylinders against falling (chaining).  Transport gas cylinders with a cylinder cart. The valve of the gas cylinder always must be closed before screwing on or unscrewing the gas hose.

WARNING Opening the cylinder valve when the cylinder is not connected. Sudden release of the stored pressure energy. Risk of injury.  Close the gas cylinder valve before connecting or removing the gas hose. After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or diluted soap solution).

1.8

Intended use

Series CB incubators are suitable for the cultivation of mammal cells under typical conditions of approx. 37 °C / 98.6°F. The incubator permits setting defined pH conditions by common NaHCO3 buffer systems of commercial cell media by keeping an exact CO2 atmosphere inside. CB incubators guarantee high humidity inside to avoid osmolarity increasing caused by the evaporation of the cell media. With the unit with O2 control, a variable oxygen atmosphere can additionally influence the growth of the cells. Observing the instructions in this operating manual and conducting regular maintenance work (chap. 13) is part of the intended use. Other applications are not approved. WARNING: If customer should use a BINDER chamber running in non-supervised continuous operation, we strongly recommend in case of inclusion of irrecoverable specimen or samples to split such specimen or samples and store them in at least two chambers, if this is feasible. CB (E3) 12/2012

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Always use proper protective equipment (clothing, gloves, safety glasses, etc.). Always follow good hygienic practices according to GLP/SOP protocols. Each individual operating the series CB incubator is responsible for his or her own safety

2.

Unit description

The CO2 incubators CB are equipped with a multifunctional microprocessor display controller with 4channel technology for temperature, CO2, and O2 (unit with O2 control) levels and a digital display accurate to one-tenth of a degree resp. 0.1 vol.-% The inner chamber, the pre-heating chamber and the inside of the doors are all made of stainless steel (material no. 1.4301 (V2A) in Germany). The inner surfaces are smooth and therefore easy to clean. The inner chamber is deep-drawn from one piece, polished (suitable for pharmaceutical applications) and has no welds or inaccessible corners. The hinges and the seal of the inner glass door are glued from the outside to aid cleaning of the inner chamber. When operating the chamber at high temperatures (sterilization), the impact of the oxygen in the air may cause discoloration of the metallic surfaces (yellowish-brown or blue) by natural oxidation processes. These colorations are harmless and will in no way impair the function or quality of the unit. The perforated shelves are also made of stainless steel. You can insert a maximum of 3 (CB 53), 6 (CB 150), resp. 8 (CB 210) shelves. The housing is RAL 7035 powder-coated. All corners and edges are also completely coated. The heating system of the CO2 incubator permits hot-air auto-sterilization at a set point of 187.5 °C / 369.5°F. Thus, a temperature of 180 °C / 356°F is maintained for at least 30 minutes on all internal surfaces, resulting in sterilization of the entire inner chamber. Therefore, this procedure meets all international guidelines regarding hot air sterilization, e.g. AAMI ST63, DIN 58947, European Pharmacopoeia. Thanks to the standard safety device (class 3.1 according to DIN 12880), the set temperature is maintained in case of failure. The gas enters the chamber via a fine filter (aseptic filter) with a high filtration efficiency that also filters the smallest particles. A highly precise, drift-free CO2 infrared measuring system in combination with the permanent mixture of CO2 gas through a special proprietary gas mixing head developed by BINDER allows precise and constant CO2 concentrations for long periods. This creates optimum growth conditions for cultures. The CO2 measuring system can be removed from the inner chamber by hand and cleaned with suitable detergents if needed. The CO2 incubator is also available with O2 control in addition to CO2 control. For options, see chap. 16.5. The CO2 incubators CB are equipped with a serial interface RS 422 for computer communication, e.g. with the communication software APT-COM™ 3 DataControlSystem (option, chap. 9.6) Temperature range: • CB 53: by 5 °C / 9°F above ambient temperature up to +60 °C / 140°F • CB 150 / CB 210: by 7 °C / 12.6°F above ambient temperature up to +60 °C / 140°F CO2 range: 0 vol.-% up to 20 vol.-%) O2 range (unit with O2 control): 0.2 vol.-% up to 95 vol.-%

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2.1

Unit overview (A)

(B) (C) (D) (E) (G)

(H)

FRONT

(I) (J) (K) (L) Figure 3: CB 150

(C) (D) (E) (D2) (F)

Figure 4: CB 53 with O2 control

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(B) (C) (D) (D2) (F) (G)

(E) Figure 5: CB 150 / CB 210 with O2 control and options (A)

Instrument panel with MB1 display controller, indicating temperature and CO2 as well as O2 (unit with O2 control)”

(B)

Connection socket for extra-low voltage supply (option, chap. 9.4)

(C)

CO2 sensor

(D)

Gas mixing head CO2

(D2) Additional gas mixing head O2/ N2 (unit with O2 control) (E)

Pt 100 temperature probe

(F)

O2 sensor (unit with O2 control)

(G)

Internal socket 230V (max. 3 A) (option, chap. 9.2)

(H)

Perforated shelves, made of stainless steel

(I)

Measuring access port

(J)

Inner glass doors

(K)

Permadry™ water pans

(L)

Lower housing cover

2.2

Instrument panel for CB

(A)

(A1)

Figure 6: Triangle instrument panel (A)

Microprocessor display controller MB1

(A1)

Main power switch ON/OFF

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2.3

Control panel at the rear of the unit 20

19

3

2

4

9

6

7

5

18

17

Figure 7: Rear control panel CB 53 with O2 control and options 20

1

8

2

9

3

10 19

4

11

5

6

12 13 4 15 16

7

17

18

Figure 8: Rear control panel CB 150 / CB 210 with O2 control and options

(1)

Switch for internal socket 230 V / 115 V (option)

(2)

Miniature fuse

(3)

Connection socket for zero-voltage relay alarm output

(4)

Quick acting closure socket for N2 (unit with O2 control)

(5)

Quick acting closure socket for O2 (unit with O2 control)

(6)

Quick acting closure socket for CO2

(7)

Switch for pressure alarm O2 ON/OFF (unit with O2 control) When turning off both switches (7) and (17) together: O2 control OFF.

(8)

External socket for extra-low voltage supply (option for CB 150 / CB 210, chap. 9.4)

(9)

Power cable

(10)

RS 422 interface for computer communication

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(11)

Switch for N2 gas cylinder changer, ON/OFF (option for CB 150 / CB 210 with O2 control)

(12)

Quick acting closure socket for 2

(13)

Switch for O2 gas cylinder changer, ON/OFF (option for CB 150 / CB 210 with O2 control)

(14)

Quick acting closure socket for 2 O2 cylinder (option for CB 150 / CB 210 with O2 control)

(15)

Switch for CO2 gas cylinder changer, ON/OFF (option for CB 150 / CB 210)

(16)

Quick acting closure socket for 2

(17)

Switch for pressure alarm N2 ON/OFF (unit with O2 control) When turning off both switches (7) and (17) together: O2 control OFF.

(18)

External gas connection for 2

(19)

Connection socket for analog output 4-20 mA (option, chap. 9.3)

nd

N2 cylinder (option for CB 150 / CB 210 with O2 control)

nd

nd

nd

CO2 cylinder (option for CB 150 / CB 210)

incubator (option for CB 150 / CB 210)

(20)

Ethernet interface for computer communication (option)

3.

Completeness of delivery, transportation, storage, and installation

3.1

Unpacking, and checking equipment and completeness of delivery

After unpacking, please check the unit and its optional accessories, if any, based on the delivery receipt for completeness and for transportation damage. Inform the carrier immediately if transportation damage has occurred. The final tests of the manufacturer may have caused traces of the shelves on the inner surfaces. This has no impact on the function and performance of the unit. Please remove any transportation protection devices and adhesives in/on the unit and on the doors and remove the operating manuals and accessory equipment. Remove any protective lamination sheet on the inner metal surfaces prior to commissioning.

CAUTION Sliding or tilting of the unit. Damage to the unit. Risk of injury by lifting heavy loads. ∅ Do NOT lift or transport the unit using the door handle, the door or the lower housing.  Lift the unit from the pallet at the four lower corners with the aid of four people.

If you need to return the unit, please use the original packing and observe the guidelines for safe lifting and transportation (chap. 3.2). For disposal of the transport packing, see chap. 14.1.

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Note on second-hand units (Ex-Demo-Units): Second-hand units are units that were used for a short time for tests or exhibitions. They are thoroughly tested before resale. BINDER ensures that the chamber is technically sound and will work flawlessly. Second-hand units are marked with a sticker on the unit door. Please remove the sticker before commissioning the unit.

3.2

Guidelines for safe lifting and transportation

After operation, please observe the guidelines for temporarily decommissioning the unit (chap. 14.2). Empty the Permadry™ water pan before moving the incubator. In case of any spilling of the contents, shut down the incubator and dry it out carefully and completely.

CAUTION Sliding or tilting the unit. Damage to the unit. Risk of injury by lifting heavy loads.  Transport the unit only in its original packaging.  Secure the CO2 incubator with transport straps for transport. ∅ Do NOT lift or transport the unit using the door handle, the door or the lower housing.  Lift the unit at the four lower corners with the aid of 4 people and place it on a rolling pallet.  Move the unit to the desired location and lift it from the rolling pallet with the aid of four people. • Permissible ambient temperature range for transport: -10 °C / 14°F to +60 °C / 140°F. You can order transport packing and rolling pallets for transportation purposes from BINDER Service.

3.3

Storage

Intermediate storage of the unit is possible in a closed and dry room. Observe the guidelines for temporary decommissioning (chap. 14.2). • Permissible ambient temperature range for storage: -10 °C / 14°F to +60 °C / 140°F. • Permissible ambient humidity: max. 70 % r.H., non-condensing When after storage in a cold location you transfer the unit to its warmer installation site, condensation may form. Before start-up, wait at least one hour until the CO2 incubator has attained ambient temperature and is completely dry.

3.4

Location of installation and ambient conditions

Notes on the location of installation Set up the CO2 incubator on a flat, even surface, free from vibration and in a well-ventilated, dry location. The chambers are designed for setting up inside a building (indoor use). Freestanding CB incubators are suitable for installation on tables or on the optionally available stand (height 200 mm / 0.5 ft). Note: The site of installation must be capable of supporting the unit’s weight (see technical data, chap. 16.4). Align the unit using a spirit level to ensure even covering of the cell-cultures with the medium. For this purpose, manually adjust the four incubator feet.

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CO2 incubators can be stacked on top of each other (two units maximum). For safe stacking that is easy to maintain, use the original BINDER stacking stand (chap. 9.10.1) or the stacking adapter (chap. 9.10.2).

CAUTION Sliding of stacked unit. Damage to the units.  When stacking, use rubber pads (Art. No. 8012-0376) for the feet of the upper incubator. To completely separate the unit from the power supply, you must disconnect the power plug. Install the unit in a way that the power plug is easily accessible and can be easily pulled in case of danger. In order to avoid contamination, never place the unit directly on the floor.

CAUTION Danger of overheating. Damage to the unit. ∅ Do NOT set up units in non-ventilated recesses.  Ensure sufficient ventilation for dispersal of the heat. Do not install or operate the CO2 incubator CB in potentially explosive areas.

DANGER Explosion hazard. Danger of death. ∅ Do NOT operate the unit in potentially explosive areas.  KEEP explosive dust or air-solvent mixtures AWAY from the vicinity of the unit. Ambient conditions • Permissible ambient temperature range for operation: CB 53: +18 °C / 64.4 °F to +32 °C / 89.6 °F CB 150 / CB 210: +18 °C / 64.4 °F to +30 °C / 86 °F At elevated ambient temperature values, fluctuations in temperature can occur. • Ideal ambient temperature CB 53: at least 5 °C / 9°F below the intended working temperature. E.g., working temperature 37 °C / 98.6°F = ambient temperature 32 °C / 89.6°F and lower. In the event of working temperatures of less than 5 °C / 9°F above the ambient temperature, the set point can be exceeded. • Ideal ambient temperature CB 150 / CB 210: at least 7 °C / 12.6°F below the intended working temperature. E.g., working temperature 37 °C / 98.6°F = ambient temperature 30 °C / 86°F and lower. In the event of working temperatures of less than 7 °C / 12.6°F above the ambient temperature, the set point can be exceeded. The ambient temperature should not be substantially higher than the indicated ambient temperature of +25 °C / 77°F to which the specified technical data relates. For other ambient conditions, deviations from the indicated data are possible. Avoid direct solar radiation on the unit. • Permissible ambient humidity: 70 % r.H. max., non-condensing. • Installation height: max. 2000 m / 6561.7 ft above sea level. After the incubator has been turned on for the first time, enter the altitude of the site above sea level into the controller (chap. 6.5). • Wall distances: rear 100 mm / 3.94 in, sides 50 mm / 1.97 in. CB (E3) 12/2012

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