User Manual Supplement
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![BacT/ALERT® 3D Version B.40 Release 4 Customer Communication bioMérieux and the blue logo, and BacT/ALERT are used, pending and/or registered trademarks of bioMérieux in the USA and other countries. © 2013 bioMérieux, Inc. All rights reserved. The following information about the release of the new BacT/ALERT® 3D Version B.40 Release 4 firmware is not contained in any of the user documentation. Please read all information provided and consult your local bioMérieux representative for further information. Reloading Positive Bottles Smear and subculture all bottles determined positive by BacT/ALERT. • If a positive bottle is reloaded within 10 minutes of being unloaded, the bottle status remains positive and the Status Determination Code is 250. • If the bottle is reloaded more than 10 minutes after being unloaded, the status reverts to negative-to-date. The BacT/ALERT system algorithms are looking for the organism’s log phase growth curve. If a positive bottle is reloaded at the end of, or after, the log phase growth curve, the bottle may or may not be flagged positive again. Therefore, true positive bottles that are reloaded may or may NOT flag positive on the BacT/ALERT system again. The subculture made at the initial positive flag should be relied upon for the culture results. CAUTION: Failure to perform a smear and subculture after identifying a positive anonymous bottle, or before reloading a positive bottle, may lead to a false negative result. 9305502 [01] 1/1](https://public.bioclinicalservices.com.au/library/representations/proxy/eyJfcmFpbHMiOnsiZGF0YSI6ODkxNSwicHVyIjoiYmxvYl9pZCJ9fQ==--02e0bae3bf84857808b880261ec2d42df2917652/eyJfcmFpbHMiOnsiZGF0YSI6eyJyZXNpemUiOiIzMjB4In0sInB1ciI6InZhcmlhdGlvbiJ9fQ==--4214356078d1f110e449cda512a9432562da3e70/bact_alert_3_d_user_manual_supplement__ver_b-40_rel.pdf.png)
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BacT/ALERT® 3D Version B.40 Release 4 Customer Communication bioMérieux and the blue logo, and BacT/ALERT are used, pending and/or registered trademarks of bioMérieux in the USA and other countries. © 2013 bioMérieux, Inc. All rights reserved.
The following information about the release of the new BacT/ALERT® 3D Version B.40 Release 4 firmware is not contained in any of the user documentation. Please read all information provided and consult your local bioMérieux representative for further information.
Reloading Positive Bottles Smear and subculture all bottles determined positive by BacT/ALERT. • If a positive bottle is reloaded within 10 minutes of being unloaded, the bottle status remains positive and the Status Determination Code is 250. • If the bottle is reloaded more than 10 minutes after being unloaded, the status reverts to negative-to-date. The BacT/ALERT system algorithms are looking for the organism’s log phase growth curve. If a positive bottle is reloaded at the end of, or after, the log phase growth curve, the bottle may or may not be flagged positive again. Therefore, true positive bottles that are reloaded may or may NOT flag positive on the BacT/ALERT system again. The subculture made at the initial positive flag should be relied upon for the culture results. CAUTION: Failure to perform a smear and subculture after identifying a positive anonymous bottle, or before reloading a positive bottle, may lead to a false negative result.
9305502
[01]
1/1