LSB Aqua Pro Shaking Water Bath Operating Manual V1

Original instructions If you have any feedback on Grant’s products or services we would like to hear from you. Please send all feedback to: Grant Instruments (Cambridge) Ltd Shepreth Cambridgeshire SG8 6GB UK Tel: +44 (0) 1763 260 811 Fax: +44 (0) 1763 262 410 E-mail: [email protected] Contents 1 2 3 4

Use of products How to use this operating manual Product registration and warranty Safety information 4.1 Safety symbols 4.2 Safety warnings 5 Operating instructions 5.1 Unpacking instructions 5.2 Installation 5.3 Electrical supply 5.4 Selecting and configuring trays and racks 5.5 Fitting the shaking trolley and a tray 6 Operating procedures 6.1 Bath liquids 6.2 Liquid level 6.3 Run-dry protection 6.4 Operation above 60°C 6.5 Achieving high temperature accuracy when shaking is used 6.6 Use as a thermostatic bath without shaking 6.7 Emptying the baths 7 Using the shaking water bath 7.1 Bath controls 7.2 Indicators 7.3 Setting the control temperature () 7.4 Setting the shaking speed 7.5 Stopping and starting the shaking 7.6 Enabling the keypad lock 7.7 Setting the over temperature protection () 7.8 Configuring and running temperature and shaking speed presets (, ) 7.9 Running temperature presets (, ) 7.10 Setting the countdown timer () 8 Calibration 8.1 Calibration options 8.2 Single point calibration () 8.3 Dual point calibration (, ) 9 Technical specification 10 Technical tips 10.1 Which water should you use in your bath? 10.2 Troubleshooting guide 11 Warranty information LSB Aqua Pro Detailed operating manual

4 4 4 5 5 5 7 7 7 7 7 9 10 10 10 11 12 12 12 12 13 13 13 14 14 16 16 17 18 19 19 21 21 21 23 26 27 27 28 28

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12 Maintenance and service 12.1 Cleaning 12.2 Fuses 12.3 Replacing the mains cord 12.4 Routine safety tests 12.5 Service 13 Compliance

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1 Use of products The following products are covered by this operating manual: LSB Aqua Pro range: LSB12, LSB12 AUS, LSB12 US, LSB18, LSB18 AUS, LSB18 US, The products listed above are a general purpose series of thermostatically controlled linear and orbital shaking water baths designed for indoor laboratory use by a professional user.

2 How to use this operating manual This operating manual will allow you to unpack, set up and operate this water bath correctly and safely. Important safety information, symbols and warnings are listed below and should be read carefully. If there is a technical matter that this operating manual does not address, or any other questions concerning this product, please contact Grant Instruments or your local distributor who will be able to provide any additional information. English is the original language of this manual

3 Product registration and warranty The warranty for this water bath is detailed in section 8 but to register you should complete the on-line registration form at www.grantinstruments.com. Not registering your product may affect your warranty.

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4 Safety information 4.1

Safety symbols

The symbols below are marked on the equipment to indicate:

Caution: Surfaces and water can be hot during and after use Read this manual before using the bath Important safety warning Recommended operation Failure to follow may affect the performance of the equipment

4.2

Safety warnings

Read the whole of the instructions. Safety may be impaired if they are not followed.

Surfaces and water can be hot during and after use. Allow the liquid temperature to fall to a safe level before emptying. Empty the bath before moving it. This bath is only intended for use with water. Use of other fluids or heat transfer media may invalidate the warranty and present a risk of fire or explosion. Place on a stable flat surface to reduce the risk of accidental spillage. No user serviceable parts. Risk of electric shock after disassembly or operation with covers removed. Not for use in environments with a risk of flammable or explosive gases. To be operated within the limits listed in this guide Do not use the bath to heat any material that could cause a fire or any other kind of hazard. Take care when operating after a power interruption as shaking will restart at the previous speed. There are accessible moving parts in the bath. Observe safe working practises when operating a shaking bath. Users should not interfere with moving parts when in use and prevent accidental contact from items such as fingers, hair or clothing. Only use the mains cord provided or one with an identical rating. Ensure that the mains plug and the switch are easily accessible.

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A clearance of >10cm around the bath is required to ensure adequate air flow. If a potentially hazardous liquid is spilt onto the equipment, disconnect it from the power supply and have it checked by a competent person. It is the user’s responsibility to carry out appropriate decontamination if hazardous material is spilt on the equipment. Clean the outside of the equipment with a damp cloth, using water and domestic cleaning products only. The use of other chemical cleaning agents may damage the equipment. Always follow the manufacturer’s instructions and any applicable legislation about the use of potentially hazardous substances.

Before first switching on the bath please remember to fill the bath with water. Switching the bath on dry can damage the heater and could invalidate the product warranty. If the equipment has been transported or stored in cold or humid conditions, condensation may form inside it. If that could have happened, allow time (at least 2 hours at room temperature) for the condensation to evaporate before using the equipment. Restricting or interfering with the tray motion in the bath may reduce performance and reduce reliability

To preserve your water bath in peak condition consult the extra guidance listed in this manual. Failure to do so may affect your warranty. Consult online resources for additional important information.

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5 Operating instructions 5.1

Unpacking instructions

Standard equipment includes:  Shaking water bath  Shaking trolley  Drive magnet  Mains cord with plug  Gabled polycarbonate lid  Short user guide Remove packing materials carefully, and retain for future shipment or storage of the equipment. 5.2

Installation

Place the water bath on a level, non-combustible surface. Ensure that the mains plug and the switch are easily accessible. A clearance of >10cm around the bath is required to ensure adequate air flow. 5.3

Electrical supply

Check that the supply voltage marked on the serial number label, and the type of mains plug, are correct for your mains supply outlet, which must have an Earth (Ground) connection. To disconnect the equipment from the mains supply, remove the mains plug from the mains supply outlet. 5.4

Selecting and configuring trays and racks

Different types of shaking trays are available to hold different vessel types. These need to be purchased separately. See www.grantinstruments.com for a full list of accessories.

Part number TU12 TU18 TF12 TF18 TS12 TS18

Description Universal tray with adjustable springs, for LSB12. Highly versatile for a variety of vessel types Universal tray with adjustable springs, for LSB18. Highly versatile for a variety of vessel types Flask tray with threaded holes to hold 25 - 1000ml flask clamps, racks and deep well plate holder (see options below) for LSB12 Flask tray with threaded holes to hold 25 - 1000ml flask clamps, racks and deep well plate holder (see options below) for LSB18 Test tube tray compatible with SR test tube and microtube racks (see options below) for LSB12. Holds up to 3 SR racks Test tube tray compatible with SR test tube and microtube racks (see options below) for LSB18. Holds up to 5 SR racks

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Flask clamps and plate holder Part number Description SC-25 For 25ml flask SC-50 For 50ml flask SC-100 For 100ml flask SC-250 For 250ml flask SC-500 For 500ml flask SC-1000 For 1000ml flask SH-DWP For 1 x deep well plate (≥2ml) Test tube rack /microtube rack Part number SR-10 SR-13 SR-16 SR-19 SR-25 SR-30 Part number SR-SE SR-LE

TF12 capacity 18 14 9 5 4 2 2

Tube diameter (mm) 10 13 16 19 25 30 Micrstube size (ml) 0.5 1.5

TF18 capacity 33 26 17 14 6 4 4

Rack capacity 48 44 24 21 12 10 Rack capacity 119 48

Configure the tray to hold in place the type of vessel being used. The number flasks and vessels that can be fitted to each tray along with the suggested position of holding springs and flask clamps is available online at www.grantinstruments.com

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5.5

Fitting the shaking trolley and a tray

Tray

Trolley

Drive magnet

Locate the trolley arm pin into the hole on the drive magnet The tray can now be lowered directly on to the trolley and will be held in place

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6 Operating procedures 6.1

Bath liquids

Water is the only liquid permitted for use in the LSB Aqua Pro. See section 7.1 for more information 6.2

Liquid level

The best liquid level for your bath depends on the application and several factors must be considered:   



When the shaking function is not in use the bath will provide optimum performance when filled to the swage line which is typically 25mm below the top of the tank. The water level used in the tank will influence the temperature accuracy and stability. Using liquid levels below the swage line needs consideration, especially when operating at higher water temperatures (>50ºC) and without a lid. When the shaking function is used the optimum liquid level depends on: o The tray type and vessel type in use. o The shaking mode and shaking speed o The minimum level which keeps the samples covered all the time and therefore allows their temperature to be properly controlled For a given shaking setting (speed/stroke length/orbital or linear) there will be a practical maximum level above which fluid from the bath will pushed over the sides of the bath by the motion of the vessels.

Do not fill above the swage line. Grant recommends the following minimum fill levels Bath LSB12 LSB18

Recommended minimum fill 5 litre 7.5 litre

Approximate water depth 60mm 60mm

Table 1 - Recommended minimum fill levels

Do not attempt to use your bath without water in the tank. The bath has an inbuilt protection mechanism known as dry start protection which will detect if the bath is started without any water, in most circumstances, and prevent the bath from continuing to heat. In this instance the bath will display  and sound an alarm. Switch the bath off before filling it with water and switching on again. The bath will not resume operation without being turned off and on again..

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The tank internal surface can become very hot if an accidental dry start has occurred, even if the dry start cut out has operated. Avoid touching the tank until it has been left to cool for several minutes. Refill carefully, a hot heater can cause a spattering of very hot water droplets and scalding steam

Repeated dry starting of the bath stresses key components in the bath which can affect service life and the equipment’s warranty.

Allowing the bath to run dry Always take care to avoid allowing the water to evaporate to the point that the bath runs dry. This can lead to the bath’s internal safety cut-out operating requiring a suitable qualified technician to reset it.

6.3

Run-dry protection

The bath has a built-in advanced detection mechanism to greatly reduce the chances of the safety cut out occurring in most circumstances where the bath is left to accidentally run dry. This feature is only enabled automatically when the following criteria are met: 

The set temperature is >50°C



The bath has been operating for a least one hour



The water is not set to boiling point (>=99ºC)

If the bath detects signs that the bath water level may be becoming low, it will alert the user by displaying  and sounding an alarm Once you have checked the water level and topped up the water level as necessary you will need to switch the bath off and on in order to resume operation. For users with specialist applications where this feature may be unnecessarily triggered it can be disabled. Press the set key to enter the bath menu and use the arrow keys to select  (Dry Protection Alarm). Press the set key and use the arrow keys to select off. Use the set key to confirm that the dry start and run dry protection are switched off. The bath also includes an independent safety temperature cut-out which will protect the bath in the unlikely event of a fault or if the dry protection alarm is switched off. If the cutout is activated then the bath will stop heating, show  on the display and sound an alarm. The bath should be switched off, unplugged and allowed to cool for at least 30 minutes. The cut-out can only be reset by a suitably qualified technician who must verify that the bath has not been damaged.

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6.4

Operation above 60°C

The lid must be used above 60°C to maintain proper temperature control and to ensure that the water temperature reaches the set point. The lid will also prevent excessive evaporation that requires the bath to be filled more often and will save energy. The lid should only be lifted by the handle, as other parts can become hot during use. It also has a vent/thermometer hole – this hole should not be sealed as pressure could build up inside the bath. 6.5

Achieving high temperature accuracy when shaking is used

To achieve higher accuracy and temperature stability, Grant recommends the bath is calibrated with the bath running at the shaking speed typically used. Single and dual point calibration options can be set. See Section 8. When operating above 70°C, Grant recommends a minimum shaking speed of 50 rpm.

Restricting or interfering with the tray motion in the bath may reduce performance and reduce reliability

6.6

Use as a thermostatic bath without shaking

If the shaking trolley and drive magnet are lifted out, a shaking bath can be used as a simple thermostatic bath. When used in this way, if flat bottomed vessels or objects are to be placed in the bath, always use a steel base tray (optional accessory) to avoid damage to the under-tank heater.

6.7

Emptying the baths

Allow the liquid temperature to fall to a safe level before emptying. Take reasonable precautions to prevent accidental spillage. If the bath is drained at temperatures above 50°C then the drain mechanism will be damaged and will need to be replaced.

Baths have drain taps to make emptying easier. To empty the bath using the drain tap, push the supplied drain insert into the drain tap. Note that the water will begin to empty as soon as the drain insert is fully engaged. A length of hose can be added to the barbed end of the drain insert if required.

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7 Using the shaking water bath 7.1

Bath controls

Temperature and Display

Shaking speed rpm button

Shaking ON/OFF button

Up, down and set keys

Alarm indicator

Preset button

Heater indicator

Over temperature protection OTP button

Figure 1 – LSB Aqua Pro front panel controls

The mains on/off switch is at the rear of the unit 7.2

Indicators

There are three indicators:  Main display – used to show temperature, shaking speed and short messages  Heater – Illuminated when the bath is heating  Alarm - flashes red when activated. A buzzer also sounds.

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7.3

Setting the control temperature ()

The water temperature of the bath can be set using the main display. The following example shows setting the water bath to 37.0°C.

. . . Figure 2 - Setting the control temperature

7.4

Setting the shaking speed



Press the rpm (revolutions per minute) button



Select speed between 20 and 200. The trolley will immediately start to move at the displayed speed

.

The speed value will automatically be saved. Press set or rpm button to quickly store the displayed speed

Figure 3 - Setting the shaking speed

The shaking speed can be checked at anytime by pressing the rpm button. The speed is displayed for 2 seconds. The bath shaking speed is dependent on how much the tray is loaded. If the shaking speed is set too high for a given tray load then the motion will judder and become erratic. If this happens, either reduce the shaking speed or the tray load until the motion becomes smooth and consistent.

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The following graphs show the shaking speed range achievable under certain tray loads and can be used for guidance. The graphs were created using Erlenmeyer flasks with a fill depth of 90mm. Factors such as fill level and vessel type will influence the maximum speed achievable. Best performance will be achieved where vessels are held securely.

LSB12

LSB18

If the shaking speed is changed by more 40rpm during operation, allow half an hour for the bath liquid temperature to stabilise. When above 70°C the recommended minimum shaking speed is 50 rpm to maintain temperature accuracy and stability.

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7.5

Stopping and starting the shaking

 

To stop the shaking, press button once

To start the shaking press button once

Figure 4 – Stopping and starting the shaking

The shaking will start at the speed previously set using the rpm button. If no speed has been set then the display will show when start is pressed. 7.6

Enabling the keypad lock

The keypad lock is intended to help users avoid accidental changes to the set point temperature of the bath. To enable and disable the lock, press and hold the set and up or down keys for three seconds as shown below

Locked

Unlocked

Figure 5 - Locking and unlocking the keypad

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7.7

Setting the over temperature protection ()

The over temperature protection can be used to protect samples by setting a maximum temperature limit the bath is allowed to heat to. If the bath exceeds this temperature, it will stop heating, display  (over temperature alarm) and sound an alarm. The alarm can be silenced by pressing the set key. Allow the bath to cool below the over temperature value to resume normal operation. Note that if the cause of overheating is an incorrect set point, this will need to be corrected otherwise the alarm will operate again. You should set the over temperature value, allowing for a safety margin to the sample maximum temperature limit if possible. Additionally the  limit should be greater than the bath set point to avoid nuisance alarms. Grant recommends this is at least 1°C. To set the over temperature alarm:

 . .

If an over temp value has already been set the display will show the value and not off.

Set the value you wish to use – in this case 40ºC

The OTP is now set and the bath will display the current water temperature

Figure 6 - Setting the over temperature alarm

To disable the alarm, set the over temperature alarm limit to 10.0°C and then press the down button one further time so the display shows . Save this by pressing the over temperature alarm key.

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7.8

Configuring and running temperature and shaking speed presets (, )

Temperature and shaking speed presets allow you to conveniently store bath temperature and speed settings you routinely use. The bath has 2 presets, numbered , .

Before you can use presets you need to store the values you wish to use in at least one of the presets using the following sequence:

 .   1



Press P. Preset 1 temperature can now be configured Set the value you wish to use – in this case 37ºC

Press P to store the value. Shaking speed preset 1 can now be configured Set the value you wish to use – in this case 120 rpm

Press the P to store the value. Preset 2 temperature can now be configured

.

Set the value you wish to use – in this case 75ºC



Press the P to store the value. Shaking speed preset 2 can now be configured



Set the value you wish to use – in this case 150rpm

 

Press the P to store the value. The display and shaking speed will return to the value before preset configuration was started

Figure 7 - Configuring a preset value

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7.9

Running temperature presets (, )

Running Preset 1

 .

The Preset temperature is displayed momentarily before returning to the current bath temperature

Running Preset 2

X3

 

The Preset temperature is displayed momentarily before returning to the current bath temperature

Figure 8 – Running a preset

7.10 Setting the countdown timer () A countdown timer can be set in the range of 1 to 999 minutes. The countdown timer will sound an alarm at the end of a countdown period. It can be used to time experiments or remind you to take a further action. Note the countdown timer does not take into account if the bath has not reached the set temperature. If the bath has not reached the set temperature, this will need to be taken into account when starting the countdown timer.

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When the countdown timer expires the bath will sound an alarm and display . Press set to silence the alarm.

To set the countdown timer:

Select the menu option

  . .

Select the countdown timer menu option

If a countdown timer value has already been set the display will show the value and not off. Set the value you require – in this case 10 minutes

Press the set key to start the timer. The remaining time will be displayed in minutes and seconds m.ss

 >10 mins

..

Or if the countdown period is greater than 10 minutes the bath with automatically return to displaying the water temperature. An extra dot at the end of the temperature will flash to show the timer is operating

Figure 9 - Setting the countdown timer

To find out the water temperature whilst the display is showing the remaining time, press the set button. To enter the configuration menus whilst the display is showing the remaining time, press the set button twice. The timer can be turned off at any time by selecting the  menu option and pressing the down arrow button until the display shows .

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