G85 Bench Top Incubator Instruction Manual Issue 2

Instruction Manual - G85

Table of contents 1

GENERAL INFORMATION & SERVICE ...3

2

UNPACKING AND INSPECTION ...4

3

ACCESSORIES ...4 3.1 3.2

4

USER MANUAL...6 4.1 4.2

5

5.3 5.4 5.5 5.6 5.7

5.8

5.9

ASSEMBLING THE G85 ...7 CONNECTION TO THE MAINS ...7 5.2.1 Start of apparatus...7 KEYBOARD FUNCTIONS ...8 OPERATING THE HEATED COMPARTMENT ...9 TEMPERATURE SETTING AND CONTROL ...9 COMBINATION KEYS ...9 GAS FLOW SETTING ...10 5.7.1 Why gassing?...10 5.7.2 Working gas pressure...10 5.7.3 Humidifying the gas mixture ...11 5.7.4 Purge function ...13 TEMPERATURE/FLOW ALARM...14 5.8.1 Temperature alarm...14 5.8.2 Gas flow alarm ...14 WARMING UP ...15

MENU FUNCTION...16 6.1 6.2 6.3 6.4 6.5 6.6 6.7 6.8 6.9 6.10 6.11

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DEFINITION OF USE ...6 GENERAL DESCRIPTION ...6

FEATURES AND OPERATION ...7 5.1 5.2

6

STANDARD ACCESSORIES ...4 OPTIONAL EQUIPMENT ...5

OVERVIEW OF OPTIONS ...17 UNIT ...18 RS232 ...19 TUNE ...20 INT.T...21 TI.ST ...21 ST.ST ...22 HEAT ...23 A-ST ...24 HOUR ...25 REST...26

USER MAINTENANCE ...27 7.1 7.2

CLEANING ...27 CALIBRATION ...27

8

TROUBLE SHOOTING ...28

9

TECHNICAL DATA...29

10

LIMITED WARRANTY...30

11

LIABILITY ...31

12

REPLACEMENT ...31

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Instruction Manual - G85

1 General Information & Service COPYRIGHT This manual contains information that is subject to copyright. All rights reserved. This manual should not be photocopied, otherwise copied or distributed, completely or in part, without the approval of K-SYSTEMS Kivex Biotec. Users of K-SYSTEMS Kivex Biotec products should not hesitate to contact us if there are any unclear points or ambiguities in this manual. K-SYSTEMS Kivex Biotec A/S Klintehøj vænge 3-5 3460 Birkerød Denmark Tel.: +45 45 99 56 00 Fax: + 45 45 99 56 19 E-mail: [email protected] Internet: www.K-SYSTEMS.dk Issue 2 Changenote No: 422 Service address: Please contact your local K-SYSTEMS Kivex Biotec distributor for details of your nearest authorized service agent.

CAUTION If the equipment is used in a manner not specified by this manual, the safety of the user may be at risk and the equipment may be damaged. Always use the equipment as outlined in this instruction manual.

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Instruction Manual - G85

2 Unpacking and inspection •

Unpack the equipment.

•

Check the packing list to insure all accessories are there.

•

Examine the packing material carefully for separately packed items.

•

Check the incubator for external surface damage.

•

Check that the display is not broken or damaged.

•

• Check that the lids and gas flow regulator knob operate correctly without any hindrance.

3 Accessories 3.1 Standard accessories 24 volts main adapter (PSU) Order code: 51122

Mains Cable Standard Schuko – Oder code: 52768 UK – Order code: 53886 US – Order code: 52773 Silicone tube Order code: 11066

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Instruction Manual - G85

3.2 Optional equipment Optional equipment consists of a range of warming blocks. Please contact your local distributor or K-SYSTEMS Kivex Biotec for details about the full range of warming blocks. Warming block B16 Holds two culture dishes B16F – Falcon 353001 Order code:26059 B16N – NUNC 150318 Order code: 26060 Warming block B24 Holds one center well dish Falcon 353653 Order code: 26027 Warming block B25 Holds one 60 mm culture dish Falcon 353002 NUNC 150288 Order code: 26028 Warming block B26 Holds one 4-well Falcon dish with lid Falcon 353654 Order code: 26031 Warming block B28 Holds one 4-well dish NUNC 176740 Order code: 26029 Warming block B29 Holds one 5-well dish MiniTüb 19021/0005 Order code: 26030

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Instruction Manual - G85

4 User Manual 4.1 Definition of use The K-SYSTEMS Kivex Biotec G85 CO² Bench Top incubator is designed for keeping gametes and embryos in a controlled environment.

4.2 General description The chambers and aluminum warming blocks maintain a preset temperature in the range ambient to 42° C. The temperature is electronically controlled by a PID controller and can be read on the displaying increments of 0.1° C. The G85 features heated lids. The bottom part is made from thick aluminum that gives ideal temperature distribution. Back part that houses the electronic is made from stainless steel. Interchangeable aluminum Warming blocks holding a variety of culture dishes are available. The G85 features a built in digital flow meter with a possible flow of 0 - 29 liters per hour and a humidification system. The G85 has also a purge function enabling a quick CO2 level recovery within the chambers. The recovery time is 15 seconds with a pressure of 0.5 bar. Note

The atmosphere in the chambers may be controlled by flushing with a gas mixture. Use only 5% CO2 in air.

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Instruction Manual - G85

5 Features and operation 5.1 Assembling the G85 No assembly needed. Just follow directions in section 5.2 and the unit is ready for use.

5.2 Connection to the mains The mains voltage is automatically regulated to the correct level by the main adapter (PSU). Connect the G85 to the mains supply using the enclosed PSU. For safety reasons the unit has an external PSU which operates at a low voltage. 5.2.1

Start of apparatus

Connect the unit to the PSU and the PSU to the mains wall socket. To power up the unit, press the heat on button on the keyboard. The display will indicate the current temperature. The aluminum dial on the side of the G85 can be turned and the gas flow level is adjusted. The valve controlling the flow is a precision valve with a short span from closed to open, so adjust with slow movements. A hint for adjusting is to close it fully and then open it gently.

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Instruction Manual - G85

5.3 Keyboard functions

Purge

Heat ON/Off

LED Display

Setpoint

Arrow keys UP/DOWN

Alarm

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Instruction Manual - G85

5.4 Operating the heated compartment Action Press heat ON/OFF key to show the actual temperature.

Key

5.5 Temperature setting and control Action Press setpoint key and the set temperature is shown.

Key

Press and hold setpoint key. While holding the setpoint key in, press either the arrow UP or arrow DOWN key to raise or lower the set temperature. When the desired temperature is reached, let go of the setpoint key. Note The setpoint can be adjusted to a reading between ambient and 49.9 ˚C.

5.6 Combination keys Action Press alarm and UP key.

Key

Display The keyboard is locked.

Press alarm and UP key.

The keyboard is unlocked.

Press Heat ON/OFF and setpoint key.

Switches between temperature and time.

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Instruction Manual - G85

5.7 Gas flow setting The display can switch between temperature and flow rate. The flow rate is shown in the display by the integrated digital flow meter in the G85. Action Press arrow key either up or down one time.

Key

Now the flow rate in liter per hour will be shown. The flow rate can be adjusted between 0 – 29 l/h. If the flow becomes larger that 29 l/h the display will indicate “Hi” (see section 5.8). Action Press arrow key either up or down once again to change to the display of temperature. 5.7.1

Key

Why gassing?

Most of the culture media which are used in assisted reproduction techniques are sensitive to changes in pH. Culture media must be maintained at a pH about 7.4. This can be obtained by gassing with atmosphere of 5% or 6% CO2. 5.7.2

Working gas pressure

Working gas pressure (inlet pressure on the G85) must be regulated to 0.5 bar by a reduction valve. The reduction valve must be a high quality unit of the pressure type that can be adjusted precisely in the 0-1 bar range. The flow rate on the G85 must be adjusted to a specific level in accordance with the use of the unit. Frequent openings of the lids will require higher flow rates. Note Any use of flow type regulators or inadequate quality pressure type regulators will cause the flow on the G85 to be unstable and drift. Below is listed the full recovery rates for CO2 level in both chambers in minutes. This can be used as guideline for the use. Flow (l/h) * Time (min) **

10 12

28 2.8

50 0.9

*Flow setting in display of G85. **Time it will take to recover CO2 level after a chamber has been opened at the above flow setting.

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Instruction Manual - G85

If the use necessitates low recovery times, either a constant high flow must be used or purging the chambers by setting the flow level at a high rate for 2-3 minutes. A flow of 10 l/h will be sufficient to sustain the CO2 levels in the chambers when the lids are not opened. The flow rate should at no time be kept under 8 l/h. A flow rate of 15-20 l/h should be adequate under most conditions without the use of manual purge. 5.7.3

Humidifying the gas mixture

In order to avoid Gassing with cold and dry Gas, the built in humidifier can be used. Connect the tubes as show on the picture below.

Warning Always route the gas as shown or permanent damage to the airflow sensors may occur. If the humidification system is not used and the gassing is done with dry gas, a silicone tube U loop must be connected between the outlet and inlet for the humidification bottle. K-SYSTEMS have experienced a quantity of failures on the flow sensors due to liquid entering the sensor and damaging it. The fault is registered by the user getting a “0” reading in the display, when an obvious flow can be seen as bubbles in the humidification flask. When adjustment in the flow rate is done on the regulator valve on the side of the G85, no changes can be seen to the flow reading in the display. It will stay at “0” disregarding the flow.

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Instruction Manual - G85

The fault occurs because of under pressure in the gas supply when the G85 is not in use. If the user shuts off the gas supply only on the bottle or the external regulator an under pressure will develop. The under pressure will draw back liquid from the humidification bottle through the gas outlet to bottle tube and into the G85 where it will damage the flow sensor. If the user remembers to completely close the regulator valve on the side of the G85 no such problem will occur. •

Alternatively the exterior 5% CO2 supply tube should be disconnected from the G85 when the G85 is not in use.

•

The Gas outlet to bottle tube disconnected when the G85 is not in use.

Or:

There is no recommended hardware update at this time, as such an under pressure cannot be avoided with certain types of gas regulators. We have considered the addition of a one-way flow valve in the circuit, but test have shown that under unfortunate circumstances the process can still be triggered. The only fully safe way to avoid the problem is as described above.

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Instruction Manual - G85

A notice or warning sticker could be added to the G85: •

Close regulator valve fully when not in use:

•

The Gas outlet to bottle tube disconnected when the G85 is not in use

Or:

There is no recommended hardware update at this time, as such an under pressure cannot be avoided with certain types of gas regulators. We have considered the addition of a one-way flow valve in the circuit, but test have shown that under unfortunate circumstances the process can still be triggered. The only fully safe way to avoid the problem is as described above. A notice or warning sticker could be added to the G85: •

Close regulator valve fully when not in use:

•

Disconnect gas outlet to bottle tube when not in use.

Or:

If the problem has occurred the gas flow sensor PCB will need to be replaced. 5.7.4

Purge function

The G85 has a purge function enabling to have a fast recovery of the CO2 level within the chambers. This function is to be used when the lid of the chambers have been opened in order to place or remove culture dishes. The purge time is 15 seconds. Action Press the alarm key to activate the purge function.

Key

When activated a “Pu” indication appears on the display.

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Instruction Manual - G85

5.8 Temperature/Flow alarm The G85 has one alarm which is activated on two events: • • 5.8.1

Temperature deviations (high or low) Gas flow Temperature alarm

The temperature alarm indicates too high or too low temperature. The alarm will be activated if the temperature rises or falls more than 0.5° C from the setpoint. There will be an acoustic signal and the red LED light in the alarm key will be activated. Action Press setpoint key and alarm key to mute the acoustic signal.

Key

Press setpoint key and alarm key once again to bring the signal back on.

Note The red LED and the acoustic signal will turn off if the temperature or gas flow stabilizes again to the setpoint level.

5.8.2

Gas flow alarm

The gas flow alarm will get activated, if the flow is higher than 29 l/h. The display will then show “hi”. The alarm will also be activated if the flow is “on”, and the heat “off”. In this case the display will show “f.on”. Note The maximum flow is 29 l/h. The G85 will not be damaged by a higher flow as long as the gas flow is kept below 100 l/h.

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Instruction Manual - G85

5.9 Warming up Warming up from 20.0 to setpoint takes less than 30 minutes, under normal conditions. When a cold item is placed in either of the chambers it may need up to 40 minutes to stabilize, depending on the thermal mass of the object and the initial temperature. G85 with two warming blocks in one chamber and humidifier show on the picture below.

Note

Always place a warming block in the chambers during warm up and be careful with the heated area under the humidifier bottle as it will become hot.

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Instruction Manual - G85

6 Menu function The user can access a number of advanced functions via the menu. Action Press and hold UP+DOWN key for 3 seconds to enter the menu.

Key

The first option “UNIT” will appear in the display. Press UP/DOWN key to navigate within the Menu. While holding the setpoint key in, press either the arrow UP or arrow DOWN key to change values within the options. Press and hold UP+DOWN key for 3 seconds again to exit the menu.

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Display

Instruction Manual - G85

The menu consists of the 10 options listed below. For further detail see the description on the following pages.

6.1 Overview of options

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Instruction Manual - G85

6.2 UNIT In the UNIT option the displayed temperature unit can be set to either degrees Celsius or Fahrenheit. Follow these steps to navigate in the UNIT option. Action Key Press and hold UP+DOWN key for 3 seconds to enter the menu. The menu "UNIT" will appear in the display. Press and hold setpoint key to change values. While holding the setpoint key in, press either the arrow UP or arrow DOWN key to select the required type of unit. When the required type has been selected let go of the setpoint key. Press and hold UP+DOWN key for 3 seconds to exit the menu.

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Display

Instruction Manual - G85

6.3 RS232 The RS232 option is for serial communication and data logging of the temperature and alarm conditions. Follow these steps to navigate in the RS232 option. Action Key Press and hold UP+DOWN key for 3 seconds to enter the menu. The option "UNIT" will appear in the display. Press DOWN key until the option “RS232” appears in the display. Press and hold setpoint key to change values. While holding the setpoint key in, press either the arrow UP or arrow DOWN key to select between "ON" and "OFF". “OFF”: The RS232 function is turned off. “ON”: Data is send once every two seconds. When the required setting is activated, let go of the setpoint key. Press and hold UP+DOWN key for 3 seconds to exit the menu.

Data logging the temperature valves during use

Note

The use of this function warrants extra equipment software. The parameters on or off will not affect normal usage when serial communication is not connected.

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Display

Instruction Manual - G85

6.4 TUNE The TUNE option is for calibration of the displayed temperature. If there is an offset between the value on the display and any measurements made with a high precision external temperature sensor, this can be corrected. The new temperature reading will be kept as the displayed value, with temperature control conducted on this basis. Follow these steps to navigate in the TUNE option. Action Key Press and hold UP+DOWN key for 3 seconds to enter the menu. The menu "USER" will appear in the display. Press DOWN key until the option “TUNE” appears in the display.

Display

Press and hold setpoint key to change values. While holding the setpoint key in, press either the arrow UP or arrow DOWN key to select the value temperature. When the required setting is activated, let go of the setpoint key. Press and hold UP+DOWN key for 3 seconds to exit the menu.



Any change in the factory set calibration should always be based on very certain temperature measurements. K-SYSTEMS Kivex Biotec only recommend the use of a high quality PT-100 sensor that is calibrated with the precision instrument used for measuring according to the manufacturers specifications. Also ensure an optimal contact between the sensor and the place measured. Use only a sensor type that is correct for the purpose and of correct size. If in doubt contact your local distributor or K-SYSTEMS Kivex Biotec.

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