MVE Operating Manual for Liquid Nitrogen Dewars Rev G

GENERAL DESCRIPTION The SC, XC and LAB series container is a vacuum insulated container of aluminum with fiberglass neck construction providing the highest efficiency possible in liquid nitrogen storage. Use the container for inert fluids only. Liquid oxygen is not compatible with fiberglass material and must not be stored. A sharp blow to the outer vessel can damage the neck tube or start a vacuum leak. Use caution and common sense in handling the container. The life expectancy of the CryoSystem Series is five (5) years. SC, XC, and Lab Series have a life expectancy of ten (10) years. Upon receipt of the container, examine both the container and packaging for any evidence of damage during shipping. Contact the shipping agent if any damage is apparent. Watch after the first fill for any signs of vacuum loss, such as frost or sweating on the outside jacket. (Some frost near the tip just after filling is normal). A. SAFETY To avoid injury by frostbite, use extreme care whenever handling liquid nitrogen, liquid nitrogen storage, transfer vessels or any objects which have come in contact with liquid nitrogen. • Leave no areas of skin exposed. • Always wear proper safety attire over clothing: face shield, cryogenic gloves, cryogenic apron. • Never overfill liquid nitrogen vessels. • Always keep liquid nitrogen vessel in an upright position. • Do not tightly seal liquid nitrogen container or prevent nitrogen gas from escaping. • Use extreme care to prevent spilling and splashing liquid nitrogen during transfer. • Immediately remove any clothing or safety attire on which liquid nitrogen has been spilled. • Get immediate medical attention for any frostbite injuries due to liquid nitrogen. WARNING: The venting of nitrogen vapors will deplete oxygen in the air, possibly leading to asphyxiation or even death. DO NOT store or use liquid container in areas that have poor ventilation. Name and address of manufacturer Authorized representative in the European Community B. FILLING INSTRUCTIONS To avoid damage to the aluminum cryogenic vessel which may result in premature vacuum loss, it is important to follow this procedure during the addition of liquid nitrogen to a warm vessel and on subsequent additions. 1. Slowly pour liquid nitrogen into new or warm vessels with a funnel or transfer line when possible. 2. Allow liquid nitrogen to settle in vessel for 2 hours to completely cool inner. 3. Fill the vessel to the desired level after the 2 hr settling (cooldown) time. 4. If filling the dewar from a pressurized source, make sure that the source tank is at a low pressure (22 psi or below). 5. If transfer hose is used for extracting liquid nitrogen from a pressurized liquid source always use a phase separator on the end of the hose. 6. Remember to always wear proper safety attire over clothing; face shield, cryogenic gloves, and apron. 7. Never overfill your dewar with liquid nitrogen. Overfilling the tank may cause pumpout components to leak leading to immediate or premature vacuum failure to occur. C. MEASURING LIQUID NITROGEN QUANTITY 1. Use wooden or plastic dipstick. Never use a hollow tube to measure liquid nitrogen. 2. Level will be indicated by frostline, which develops when dipstick is removed. D. LIQUID WITHDRAWAL 1. Liquid withdrawal from LAB units must be accomplished by pouring or utilizing a withdrawal device. Withdrawal device pressurizes to approximately 5 psi and the pressure forces liquid up the withdrawal tube out the valve. 2. Always wear proper safety attire; shield, gloves and apron. 2

WARRANTY ACTIVATION IMPORTANT! PLEASE FILL OUT THIS REGISTRATION CARD TO ACTIVATE YOUR WARRANTY YOUR NAME _____________________________________________________________________ ADDRESS _______________________________________________________________________ CITY ___________________________________ STATE _________ ZIP ____________________ NAME OF DISTRIBUTOR ____________________________________________________________ ADDRESS ________________________________________________________________________ SERIAL NUMBER ______________________________ DATE PURCHASED ___________________ What is being stored in the container?  Semen  Embryos  Skin  Vaccines  Blood  Bone Marrow  Cell Lines  Other Was the new container purchased for  Replacement  New Lab  Project  Additional Storage How did you become aware of MVE products?  Advertisement  Sales Distributor  Colleague  Trade Show  Thomas Register  Internet  Other ___________________ What Journal/Magazines are you currently receiving? (Rate 1 as most useful, 3 as less useful) 1 ________________________ 2 _________________________ 3 ________________________ How important were the following features that lead to the purchase of this product? Very Important Not Important Quality of freezer 10 9 8 7 6 5 4 3 2 1 MVE reputation 10 9 8 7 6 5 4 3 2 1 Price 10 9 8 7 6 5 4 3 2 1 Storage Capacity 10 9 8 7 6 5 4 3 2 1 Warranty 10 9 8 7 6 5 4 3 2 1 Standard Features 10 9 8 7 6 5 4 3 2 1 Customer Service 10 9 8 7 6 5 4 3 2 1 Distributor Reputation 10 9 8 7 6 5 4 3 2 1 Colleague Referral 10 9 8 7 6 5 4 3 2 1 Temperature Under Lid 10 9 8 7 6 5 4 3 2 1 Nitrogen Consumption 10 9 8 7 6 5 4 3 2 1 Serviceability 10 9 8 7 6 5 4 3 2 1 Other 10 9 8 7 6 5 4 3 2 1 How satisfied are you with your local MVE representative? Very Satisfied Not Satisfied 10 9 8 7 6 5 4 3 2 1 Comments _______________________________________________________________________ ________________________________________________________________________________ Overall, how satisfied are you with your MVE container? 10 9 8 7 6 5 4 3 2 1 Comments________________________________________________________________________ ________________________________________________________________________________ Please make any additional comments or suggestions _____________________________________ ________________________________________________________________________________

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Limited Warranty Statement Chart Inc., 2200 Airport Industrial Dr., Suite 500, Ball Ground, GA 30107

General Terms: “New Product” – Chart Inc. ("CHART") warrants to the original purchaser ("Purchaser") that each new Liquid Nitrogen Dewar (collectively, the "New Products"), shall be free from defects in materials and workmanship for a period of two (2) years from the date of shipment, except as provided below. CHART warrants that the dewar vacuum integrity shall be free from defects in materials and workmanship as listed below, except as provided in the Limited Warranty Provisions below: Spectrum and CryoSystem Series, ET11, ET24, A-200, Workhorse 2003: 3 years / XC, SC, and Lab Series, ET 23-JLM: 5 years

“Repair/Blemished Product” – CHART warrants to the Purchaser that all repaired equipment and factory blemished equipment (collectively, the "Repair/Blemished Products") shall be free of defects in materials and workmanship for a period of (90) days from the date of shipment, except as provided below. CHART warrants that the dewar vacuum integrity shall be free from defects in materials and workmanship for a period of (I) year from date of shipment, except as provided below.

“Service Part” – CHART warrants to the Purchaser that all Service Parts and Accessory Items (each a "Service Part") are warranted for (90) days from date of shipment to be free of defects in material and workmanship, except as provided below.

Limited Warranty Provisions: Purchaser agrees that before this limited warranty shall become effective, Purchaser shall fully inspect each New Product, Repair/Blemished Product, or Service Part within ten (10) days of delivery and before such Product is put to use. Purchaser also agrees to operate the New Product, Repair/Blemished Product, or Service Part in accordance with CHART's operating instructions and that failure to do so shall void this limited warranty. Purchaser further agrees that any claim for breach of warranty must be made in writing within 60 days of discovery of a purported defect. CHART will not be responsible for any alleged breach of warranty, which, as a result of CHART's inspection, CHART determines to have arisen from a cause not covered by this limited warranty. In this case, CHART will charge the purchaser a nominal fee to repair the unit.

CHART BIOMEDICAL PRODUCTS ATTN: CUSTOMER SERVICE MANAGER 2200 Airport Industrial Dr. Suite 500 Ball Ground, GA 30107 PLACE STAMP HERE

This limited warranty does not apply to: (A) Normal routine service items; (B) Repair or replacement necessitated by misuse, abuse, accident, or repairs made by persons other than CHART or persons not authorized by CHART, (C) Use of external equipment or parts with the New Product, Repair/Blemished Product, or Service Parts other than those approved by CHART, (D) Defects caused by effects of normal wear and tear; and (E) Acts of God, or other causes not within the control of CHART.

Thank You! You have just purchased the finest cryobiological storage container manufactured today. With Chart BioMedical Division’s experience and reputation, you can be assured of the most advanced technology and unsurpassed quality throughout our product. These containers have been designed, manufactured and tested to achieve maximum efficiency, along with durability to give many years of trouble-free service. If you have any questions regarding this product please contact Chart BioMedical.

If Purchaser believes that a New Product, Repair/Blemished Product, or Service Part does not comply with the limited warranty stated above, Purchaser should contact CHART at the address stated above, describing the problem and providing proof of the date of purchase. If directed by CHART, Purchaser shall return the New Product, Repair/Blemished Product, or Service Part freight prepaid, properly packaged in a CHART approved shipping container and properly identified by a Return Material Authorization Number issued by CHART. New Products, Repair/Blemished Product, or Service Parts returned without a Return Material Authorization Number will be refused and returned at Purchaser's expense. The remedies available for any breach of this limited warranty are limited to repair or replacement of the defective New Product, Repair/Blemished Product, Service Part, or refund of the purchase price, at the sole discretion of CHART. CHART warrants that replacement or repaired New Product, Repair/Blemished Product, or Service Part shall be free from defects in material and workmanship for the duration of the unexpired portion of the original warranty or ninety (90) days from the date of re-shipment to Purchaser, whichever is longer. PURCHASER'S RECOVERY FROM CHART FOR ANY CLAIM SHALL NOT EXCEED PURCHASER’S PURCHASE PRICE FOR THE NEW PRODUCT, REPAIR/BLEMISHED PRODUCT, OR SERVICE PART GIVING RISE TO SUCH CLAIM, IRRESPECTIVE OF THE NATURE OF THE CLAIM, WHETHER IN CONTRACT, TORT, WARRANTY, NEGLIGENCE, STRICT LIABILITY OR OTHERWISE. CHART SHALL NOT BE LIABLE FOR AND PURCHASER SHALL INDEMNIFY, DEFEND AND HOLD CHART HARMLESS FROM ANY CLAIMS BASED ON CHART'S COMPLIANCE WITH PURCHASER'S DESIGNS, SPECIFICATIONS OR INSTRUCTIONS, OR MODIFICATION OF ANY PRODUCTS BY PARTIES OTHER THAN CHART, OR USE IN COMBINATION WITH OTHER PRODUCTS. PURCHASER SHALL NOT IN ANY EVENT BE ENTITLED TO, AND CHART SHALL NOT BE LIABLE FOR, INDIRECT, SPECIAL, INCIDENTAL OR CONSEQUENTIAL DAMAGES OF ANY NATURE INCLUDING, WITHOUT LIMITATION, BUSINESS INTERRUPTION COSTS, REMOVAL AND/OR REINSTALLATION COSTS, REPROCUREMENT COSTS, LOSS OF PROFIT OR REVENUE, LOSS OF DATA, PROMOTIONAL OR MANUFACTURING EXPENSES, OVERHEAD, INJURY TO REPUTATION OR LOSS OF CUSTOMERS, EVEN IF CHART HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGES. EXCEPT FOR THIS LIMITED WARRANTY, CHART HAS MADE NO WARRANTIES OR REPRESENTATIONS, EXPRESSED OR IMPLIED, AND HEREBY DISCLAIMS ANY OTHER WARRANTIES, INCLUDING, BUT NOT LIMITED TO, IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. NO REPRESENTATION OR STATEMENT OF CHART MAY CHANGE OR ALTER THIS LIMITED WARRANTY. Any claims for breach of this limited warranty shall be governed by Georgia law and without regard to conflict of law rules thereof and must be brought in a state or federal court in Georgia.

4

Some states do not allow limitations on implied warranties or on incidental or consequential damages, so the above limitations may not apply. This limited warranty gives the Purchaser specific legal rights. Purchaser may also have other rights, which vary from state to state. 5

GENERAL DESCRIPTION The SC, XC and LAB series container is a vacuum insulated container of aluminum with fiberglass neck construction providing the highest efficiency possible in liquid nitrogen storage. Use the container for inert fluids only. Liquid oxygen is not compatible with fiberglass material and must not be stored. A sharp blow to the outer vessel can damage the neck tube or start a vacuum leak. Use caution and common sense in handling the container. The life expectancy of the CryoSystem Series is five (5) years. SC, XC, and Lab Series have a life expectancy of ten (10) years. Upon receipt of the container, examine both the container and packaging for any evidence of damage during shipping. Contact the shipping agent if any damage is apparent. Watch after the first fill for any signs of vacuum loss, such as frost or sweating on the outside jacket. (Some frost near the tip just after filling is normal). A. SAFETY To avoid injury by frostbite, use extreme care whenever handling liquid nitrogen, liquid nitrogen storage, transfer vessels or any objects which have come in contact with liquid nitrogen. • Leave no areas of skin exposed. • Always wear proper safety attire over clothing: face shield, cryogenic gloves, cryogenic apron. • Never overfill liquid nitrogen vessels. • Always keep liquid nitrogen vessel in an upright position. • Do not tightly seal liquid nitrogen container or prevent nitrogen gas from escaping. • Use extreme care to prevent spilling and splashing liquid nitrogen during transfer. • Immediately remove any clothing or safety attire on which liquid nitrogen has been spilled. • Get immediate medical attention for any frostbite injuries due to liquid nitrogen. WARNING: The venting of nitrogen vapors will deplete oxygen in the air, possibly leading to asphyxiation or even death. DO NOT store or use liquid container in areas that have poor ventilation. Name and address of manufacturer Authorized representative in the European Community B. FILLING INSTRUCTIONS To avoid damage to the aluminum cryogenic vessel which may result in premature vacuum loss, it is important to follow this procedure during the addition of liquid nitrogen to a warm vessel and on subsequent additions. 1. Slowly pour liquid nitrogen into new or warm vessels with a funnel or transfer line when possible. 2. Allow liquid nitrogen to settle in vessel for 2 hours to completely cool inner. 3. Fill the vessel to the desired level after the 2 hr settling (cooldown) time. 4. If filling the dewar from a pressurized source, make sure that the source tank is at a low pressure (1.52 bar or below). 5. If transfer hose is used for extracting liquid nitrogen from a pressurized liquid source always use a phase separator on the end of the hose. 6. Remember to always wear proper safety attire over clothing; face shield, cryogenic gloves, and apron. 7. Never overfill your dewar with liquid nitrogen. Overfilling the tank may cause pumpout components to leak leading to immediate or premature vacuum failure to occur. C. MEASURING LIQUID NITROGEN QUANTITY 1. Use wooden or plastic dipstick. Never use a hollow tube to measure liquid nitrogen. 2. Level will be indicated by frostline, which develops when dipstick is removed. D. LIQUID WITHDRAWAL 1. Liquid withdrawal from LAB units must be accomplished by pouring or utilizing a withdrawal device. Withdrawal device pressurizes to approximately 0.35 bar and the pressure forces liquid up the withdrawal tube out the valve. 2. Always wear proper safety attire; shield, gloves and apron. 6

WARRANTY ACTIVATION IMPORTANT! PLEASE FILL OUT THIS REGISTRATION CARD TO ACTIVATE YOUR WARRANTY YOUR NAME _____________________________________________________________________ ADDRESS _______________________________________________________________________ CITY ___________________________________ STATE _________ ZIP ____________________ NAME OF DISTRIBUTOR ____________________________________________________________ ADDRESS ________________________________________________________________________ SERIAL NUMBER ______________________________ DATE PURCHASED ___________________ What is being stored in the container?  Semen  Embryos  Skin  Vaccines  Blood  Bone Marrow  Cell Lines  Other Was the new container purchased for  Replacement  New Lab  Project  Additional Storage How did you become aware of MVE products?  Advertisement  Sales Distributor  Colleague  Trade Show  Thomas Register  Internet  Other ___________________ What Journal/Magazines are you currently receiving? (Rate 1 as most useful, 3 as less useful) 1 ________________________ 2 _________________________ 3 ________________________ How important were the following features that lead to the purchase of this product? Very Important Not Important Quality of freezer 10 9 8 7 6 5 4 3 2 1 MVE reputation 10 9 8 7 6 5 4 3 2 1 Price 10 9 8 7 6 5 4 3 2 1 Storage Capacity 10 9 8 7 6 5 4 3 2 1 Warranty 10 9 8 7 6 5 4 3 2 1 Standard Features 10 9 8 7 6 5 4 3 2 1 Customer Service 10 9 8 7 6 5 4 3 2 1 Distributor Reputation 10 9 8 7 6 5 4 3 2 1 Colleague Referral 10 9 8 7 6 5 4 3 2 1 Temperature Under Lid 10 9 8 7 6 5 4 3 2 1 Nitrogen Consumption 10 9 8 7 6 5 4 3 2 1 Serviceability 10 9 8 7 6 5 4 3 2 1 Other 10 9 8 7 6 5 4 3 2 1 How satisfied are you with your local MVE representative? Very Satisfied Not Satisfied 10 9 8 7 6 5 4 3 2 1 Comments _______________________________________________________________________ ________________________________________________________________________________ Overall, how satisfied are you with your MVE container? 10 9 8 7 6 5 4 3 2 1 Comments________________________________________________________________________ ________________________________________________________________________________ Please make any additional comments or suggestions _____________________________________ ________________________________________________________________________________

7

Limited Warranty Statement Chart Inc., 2200 Airport Industrial Dr., Suite 500, Ball Ground, GA 30107

General Terms: “New Product” – Chart Inc. ("CHART") warrants to the original purchaser ("Purchaser") that each new Liquid Nitrogen Dewar (collectively, the "New Products"), shall be free from defects in materials and workmanship for a period of two (2) years from the date of shipment, except as provided below. CHART warrants that the dewar vacuum integrity shall be free from defects in materials and workmanship as listed below, except as provided in the Limited Warranty Provisions below: Spectrum and CryoSystem Series, ET11, ET24, A-200, Workhorse 2003: 3 years / XC, SC, and Lab Series, ET 23-JLM: 5 years

“Repair/Blemished Product” – CHART warrants to the Purchaser that all repaired equipment and factory blemished equipment (collectively, the "Repair/Blemished Products") shall be free of defects in materials and workmanship for a period of (90) days from the date of shipment, except as provided below. CHART warrants that the dewar vacuum integrity shall be free from defects in materials and workmanship for a period of (I) year from date of shipment, except as provided below.

“Service Part” – CHART warrants to the Purchaser that all Service Parts and Accessory Items (each a "Service Part") are warranted for (90) days from date of shipment to be free of defects in material and workmanship, except as provided below.

Limited Warranty Provisions: Purchaser agrees that before this limited warranty shall become effective, Purchaser shall fully inspect each New Product, Repair/Blemished Product, or Service Part within ten (10) days of delivery and before such Product is put to use. Purchaser also agrees to operate the New Product, Repair/Blemished Product, or Service Part in accordance with CHART's operating instructions and that failure to do so shall void this limited warranty. Purchaser further agrees that any claim for breach of warranty must be made in writing within 60 days of discovery of a purported defect. CHART will not be responsible for any alleged breach of warranty, which, as a result of CHART's inspection, CHART determines to have arisen from a cause not covered by this limited warranty. In this case, CHART will charge the purchaser a nominal fee to repair the unit.

CHART BIOMEDICAL PRODUCTS ATTN: CUSTOMER SERVICE MANAGER Unit 2 – Maxdata Centre Downmill Road Bracknell, Berkshire RG12 1QS United Kingdom PLACE STAMP HERE

This limited warranty does not apply to: (A) Normal routine service items; (B) Repair or replacement necessitated by misuse, abuse, accident, or repairs made by persons other than CHART or persons not authorized by CHART, (C) Use of external equipment or parts with the New Product, Repair/Blemished Product, or Service Parts other than those approved by CHART, (D) Defects caused by effects of normal wear and tear; and (E) Acts of God, or other causes not within the control of CHART.

Thank You! You have just purchased the finest cryobiological storage container manufactured today. With Chart BioMedical Division’s experience and reputation, you can be assured of the most advanced technology and unsurpassed quality throughout our product. These containers have been designed, manufactured and tested to achieve maximum efficiency, along with durability to give many years of trouble-free service. If you have any questions regarding this product please contact Chart BioMedical.

If Purchaser believes that a New Product, Repair/Blemished Product, or Service Part does not comply with the limited warranty stated above, Purchaser should contact CHART at the address stated above, describing the problem and providing proof of the date of purchase. If directed by CHART, Purchaser shall return the New Product, Repair/Blemished Product, or Service Part freight prepaid, properly packaged in a CHART approved shipping container and properly identified by a Return Material Authorization Number issued by CHART. New Products, Repair/Blemished Product, or Service Parts returned without a Return Material Authorization Number will be refused and returned at Purchaser's expense. The remedies available for any breach of this limited warranty are limited to repair or replacement of the defective New Product, Repair/Blemished Product, Service Part, or refund of the purchase price, at the sole discretion of CHART. CHART warrants that replacement or repaired New Product, Repair/Blemished Product, or Service Part shall be free from defects in material and workmanship for the duration of the unexpired portion of the original warranty or ninety (90) days from the date of re-shipment to Purchaser, whichever is longer. PURCHASER'S RECOVERY FROM CHART FOR ANY CLAIM SHALL NOT EXCEED PURCHASER’S PURCHASE PRICE FOR THE NEW PRODUCT, REPAIR/BLEMISHED PRODUCT, OR SERVICE PART GIVING RISE TO SUCH CLAIM, IRRESPECTIVE OF THE NATURE OF THE CLAIM, WHETHER IN CONTRACT, TORT, WARRANTY, NEGLIGENCE, STRICT LIABILITY OR OTHERWISE. CHART SHALL NOT BE LIABLE FOR AND PURCHASER SHALL INDEMNIFY, DEFEND AND HOLD CHART HARMLESS FROM ANY CLAIMS BASED ON CHART'S COMPLIANCE WITH PURCHASER'S DESIGNS, SPECIFICATIONS OR INSTRUCTIONS, OR MODIFICATION OF ANY PRODUCTS BY PARTIES OTHER THAN CHART, OR USE IN COMBINATION WITH OTHER PRODUCTS. PURCHASER SHALL NOT IN ANY EVENT BE ENTITLED TO, AND CHART SHALL NOT BE LIABLE FOR, INDIRECT, SPECIAL, INCIDENTAL OR CONSEQUENTIAL DAMAGES OF ANY NATURE INCLUDING, WITHOUT LIMITATION, BUSINESS INTERRUPTION COSTS, REMOVAL AND/OR REINSTALLATION COSTS, REPROCUREMENT COSTS, LOSS OF PROFIT OR REVENUE, LOSS OF DATA, PROMOTIONAL OR MANUFACTURING EXPENSES, OVERHEAD, INJURY TO REPUTATION OR LOSS OF CUSTOMERS, EVEN IF CHART HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGES. EXCEPT FOR THIS LIMITED WARRANTY, CHART HAS MADE NO WARRANTIES OR REPRESENTATIONS, EXPRESSED OR IMPLIED, AND HEREBY DISCLAIMS ANY OTHER WARRANTIES, INCLUDING, BUT NOT LIMITED TO, IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. NO REPRESENTATION OR STATEMENT OF CHART MAY CHANGE OR ALTER THIS LIMITED WARRANTY. Any claims for breach of this limited warranty shall be governed by Georgia law and without regard to conflict of law rules thereof and must be brought in a state or federal court in Georgia.

8

Some states do not allow limitations on implied warranties or on incidental or consequential damages, so the above limitations may not apply. This limited warranty gives the Purchaser specific legal rights. Purchaser may also have other rights, which vary from state to state. 9