Galaxy 170 R/S Operating Manual April 2016

Copyright © 2017 Eppendorf AG, Germany. All rights reserved, including graphics and images. No part of this publication may be reproduced without the prior permission of the copyright owner. Eppendorf® and the Eppendorf Brand Design are registered trademarks of Eppendorf AG, Germany. New Brunswick™ is a trademark of Eppendorf AG, Germany. Galaxy® is a registered trademark of Eppendorf, Inc., USA. BioCommand® is a registered trademark of Eppendorf, Inc., USA. Registered trademarks and protected trademarks are not marked in all cases with ® or ™ in this manual. p. Tab. Operating Galaxy® on see Fig. p. 170 manual R/S CO2 Incubators English Operating (EN) manual

6710 900.025-03/072017 Gen. 2

Table of contents Galaxy® 170 R/S CO2 Incubators English (EN)

Table of contents 1

Operating instructions... 7 1.1 Using this manual... 7 1.2 Danger symbols and danger levels... 7 1.2.1 Danger symbols... 7 1.2.2 Danger levels... 7 1.3 Symbols used... 8

2

Safety... 9 2.1 Intended use... 9 2.2 User profile... 9 2.3 Application limits... 10 2.3.1 Description of ATEX Guideline (94/9EC)... 10 2.4 Information on product liability... 10 2.5 Warnings for intended use... 11 2.5.1 Personal injury and damage to device... 11 2.6 Warning signs on the device... 12

3

Product description... 13 3.1 Product overview... 13 3.1.1 Galaxy 170 R CO2 Incubators... 13 3.1.2 Galaxy 170 S CO2 Incubators... 16 3.1.3 Inside the chamber... 19 3.2 Delivery package... 20 3.2.1 Inspection of boxes... 21 3.2.2 Packing list verification... 21 3.3 Features... 21 3.3.1 Control system... 21 3.3.2 Direct heating system... 21 3.3.3 Infrared sensor... 21 3.3.4 Controlled humidity tray... 21 3.3.5 Seamless chamber... 22 3.3.6 Standard features... 22 3.3.7 Multiple options... 22 3.3.8 Two-level alarm system... 22 3.4 Stacking devices... 22

4

Installation... 23 4.1 Utilities requirements... 23 4.2 Selecting the location... 23 4.3 Unpacking the incubator... 24 4.4 Initial setup... 25 4.5 Making connections... 28

3

4

Table of contents Galaxy® 170 R/S CO2 Incubators English (EN)

5

Operation... 31 5.1 Preparing for operation... 31 5.2 Using the humidity tray... 32 5.3 RS-232 interface... 33 5.4 BMS relay contact alarm... 37 5.4.1 Incubator operating with and without alarm... 39 5.5 Operation for optional features... 40

6

Operating controls and function for Galaxy 170 R Incubator... 41 6.1 170 R control panel... 41 6.2 Programming... 42 6.2.1 Temperature and CO2 level... 42 6.2.2 User access code... 42 6.2.3 Removing user access code... 42 6.3 Referencing the CO2 sensor with Auto-Zero... 43 6.4 USER settings... 43 6.4.1 SET DATE AND TIME... 43 6.4.2 AUDIBLE ALARM VOLUME ADJUST... 44 6.4.3 PROGRAMMABLE CO2 AUTOZERO... 44 6.4.4 DATALOGGER... 45 6.4.5 POWER FREQUENCY... 45 6.4.6 DISABLE... 45 6.4.7 DISINFECTION (optional)... 45 6.5 DATALOGGER screen... 46 6.5.1 ALARM EVENTS... 46 6.5.2 TEMPERATURE GRAPH + DOOR OPEN BAR CHART... 47 6.5.3 CO2 GRAPH + DOOR OPEN BAR CHART... 48 6.5.4 DIAGNOSTIC CHAMBER ELEMENT GRAPH... 48 6.5.5 DIAGNOSTIC DOOR GRAPH... 48 6.5.6 DIAGNOSTIC DOOR ELEMENT GRAPH... 48 6.5.7 RESTART GRAPHIC RECORD... 48 6.6 CHAMBER ALARMS menu screen... 49 6.6.1 Chamber alarm system function... 50 6.7 DIAGNOSTICS menu screen... 51 6.8 HELP MENU screen... 52

7

Operating controls and function for Galaxy 170 S Incubator... 53 7.1 170 S control panel... 53 7.2 Setting temperature and CO2... 54 7.3 Programming the alarm system... 54 7.3.1 Setting the high and low temperature alarms... 54 7.3.2 Setting the CO2 high and low alarms... 55 7.3.3 Door open alarm... 55 7.3.4 Alarm duration... 55 7.3.5 Alarm arming delay... 56

Table of contents Galaxy® 170 R/S CO2 Incubators English (EN)

7.4

7.5

Chamber alarm system... 56 7.4.1 Temperature sensor system alarms... 57 7.4.2 Over-temperature cut-out and alarm... 57 CO2 Auto-zero system... 57 7.5.1 Auto-zero... 57 7.5.2 Changing auto-zero frequency... 58 7.5.3 High temperature disinfection operation... 58

8

Maintenance... 59 8.1 Routine maintenance... 59 8.1.1 General... 59 8.1.2 Daily Checks... 59 8.1.3 Weekly checks... 60 8.1.4 Monthly checks... 60 8.1.5 CO2 Sampling with analyzer... 60 8.1.6 Routine checks of the O2 control option (170 R only)... 61 8.2 Cleaning... 61 8.3 Disinfection/Decontamination... 62 8.4 High temperature disinfection... 64

9

Troubleshooting... 65 9.1 General errors... 65 9.2 O2 sensor... 65

10 Transport, storage and disposal... 67 10.1 Transport... 67 10.2 Storage... 67 10.3 Disposal... 68 11 Technical data... 69 11.1 Weight/dimensions... 69 11.1.1 Equipment dimensions... 69 11.1.2 Internal dimensions... 69 11.1.3 Transporting dimensions... 69 11.1.4 Shelves... 70 11.2 Power supply... 71 11.3 Ambient conditions... 71 11.4 Application parameters... 71 11.4.1 Temperature management... 71 11.4.2 CO2 control... 72 11.4.3 Relative humidity... 72 11.4.4 Calibration... 72 12 Ordering information... 73 12.1 Accessories... 73 12.2 Available options... 74

5

6

Table of contents Galaxy® 170 R/S CO2 Incubators English (EN)

13 Equipment options... 75 13.1 High temperature disinfection... 75 13.1.1 Using the high temperature disinfection (Galaxy 170 R)... 75 13.1.2 Using the high temperature disinfection (Galaxy 170 S)... 77 13.1.3 High temperature disinfection option with oxygen control... 78 13.2 O2 control (1 - 19 %) (Galaxy 170 R only)... 79 13.2.1 Setting up the N2 tank... 79 13.2.2 Setting up oxygen control... 80 13.2.3 Operating guidelines... 81 13.2.4 Referencing to atmosphere... 82 13.2.5 Replace sensor soon... 82 13.2.6 Replace sensor now... 83 13.2.7 Removing and replacing O2 sensor... 84 13.2.8 Replacing the filter disc... 86 13.2.9 Troubleshooting the O2 sensor... 87 13.2.10 Specifications... 87 13.3 O2 control (0.1 - 19 %) (Galaxy 170 R only)... 88 13.3.1 Setting up the N2 tank... 90 13.3.2 Setting up O2 control... 91 13.3.3 Enabling or disabling O2 control... 91 13.3.4 Alarms... 92 13.3.5 Referencing to atmosphere... 92 13.3.6 Programming desired O2 level... 92 13.3.7 Precautions... 92 13.4 O2 Sensor replacement (Galaxy 170 R only)... 93 13.5 Humidity alert and monitoring package (Galaxy 170 R only)... 93 13.5.1 Humidity tray warning system... 93 13.5.2 Humidity display and alarm system... 94 13.6 Copper inner chamber... 94 Index... 95 Certificates... 97

Operating instructions Galaxy® 170 R/S CO2 Incubators English (EN)

1 1.1

Operating instructions Using this manual

 Read this operating manual thoroughly before using the device for the first time. Also observe the instructions for use of the accessories.

 This operating manual is part of the product. It must always be kept easily accessible.  Enclose this operating manual when transferring the device to third parties.  You will find the current version of the operating manual for all available languages on our website at www.eppendorf.com/manuals.

1.2

Danger symbols and danger levels

The safety instructions of this operating manual indicate the following danger symbols and danger levels:

1.2.1

Danger symbols Hazard point

Hot surface

Electric shock

Material damage

Explosion

Heavy loads

Inhalation

1.2.2

Danger levels

DANGER

Will lead to severe injuries or death.

WARNING

May lead to severe injuries or death.

CAUTION

May lead to light to moderate injuries.

NOTICE

May lead to material damage.

7

8

Operating instructions Galaxy® 170 R/S CO2 Incubators English (EN)

1.3

Symbols used

Depiction

Meaning

1. 2.

Actions in the specified order



Actions without a specified order

•

List

Text

Display or software texts Additional information

Safety Galaxy® 170 R/S CO2 Incubators English (EN)

2 2.1

Safety Intended use

Galaxy 170 R/170 S CO2 incubator is designed to and serve to provide stable and homogeneous atmosphere required for cell culture by controlling temperature, carbon dioxide and optionally oxygen for cultivating samples and cells from biological laboratories. They are intended exclusively for use indoors and for operation by qualified staff for research purposes only.

CAUTION! Lack of safety due to incorrect accessories or spare parts

 Accessories and spare parts that are not recommended by Eppendorf compromise the safety, functions, and precision of the device. Eppendorf cannot be held liable or accept any liability for damage resulting from the use of non-recommended accessories.  Only use accessories and original spare parts recommended by Eppendorf.

2.2

User profile

The device may only be operated by trained lab personnel who have carefully read the operating manual and are familiar with the device functions.

CAUTION!

 This equipment must be operated as described in this manual. If operational guidelines are not followed, equipment damage and personal injury can occur. Please read the entire Operating Manual before attempting to use this unit.  Do not use this equipment in a hazardous atmosphere or with hazardous materials for which the equipment was not designed.  Eppendorf is not responsible for any damage to this equipment that may result from the use of an accessory not manufactured by Eppendorf.

9

10

Safety Galaxy® 170 R/S CO2 Incubators English (EN)

2.3

Application limits

2.3.1

Description of ATEX Guideline (94/9EC) DANGER! Explosion hazard

 Do not operate the device in areas where work is completed with explosive substances.  Do not use this device to process any explosive or highly reactive substances.  Do not use this device to process any substances which could create an explosive atmosphere.

CAUTION! Lack of safety due to incorrect use of materials

 Do not use this device to process any flammable materials, or use materials where the transfer of mechanical energy to glass apparatus could lead to breakage.

Due to its design and the ambient conditions in its interior, the device is not suitable for use in potentially explosive atmospheres. The device may only be used in a safe environment, e.g., the open atmosphere of a ventilated lab. The use of substances which may contribute to a potentially explosive atmosphere is not permitted. The final decision regarding the risks associated with using these types of substances is the user's responsibility.

2.4

Information on product liability

In the following cases, the designated protection of the device may be compromised. Liability for any resulting property damage or personal injury is then transferred to the operator: • The device is not used in accordance with the operating manual. • The device is used outside of its intended use. • The device is used with accessories or consumables which are not recommended by Eppendorf. • The device is maintained or repaired by individuals not authorized by Eppendorf. • The user makes unauthorized changes to the device.

Safety Galaxy® 170 R/S CO2 Incubators English (EN)

2.5

Warnings for intended use

Before using the device, read the operating manual and observe the following general safety instructions.

2.5.1

Personal injury and damage to device WARNING! Burns during the high temperature disinfection cycle

 Do not open equipment door during the high temperature disinfection cycle.  Do not touch the equipment during the high temperature disinfection cycle. WARNING! Risk of personal injury

 Do not open the device!  Do not operate a broken device! (e.g. if the exterior is damaged)  Do not modify the device! CAUTION! Risk of personal injury

 At least four people are required to safely lift the incubator  The incubator has a high center of gravity. Make sure it does not topple over during transportation/moving!

WARNING! Risk of personal injury and equipment damage The incubator or incubators may topple if they are not fixed with a safety latch.

 Each incubator or stack of two incubators on a stacking stand must be fixed to the wall with a safety latch.

CAUTION! Risk of personal injury

 Safety standards of lab must be considered.  Use your personal protective equipment. CAUTION! Risk of personal injury

 Before closing the glass door be sure that the shelves well installed inside the chamber. Slamming the glass door against the shelf could cause broken glass and potential injury.

NOTICE! Damage of the incubator Lifting the incubator by the door causes permanent damage to the incubator.

 Lift the incubator by its housing.  Never lift the incubator by its door.

11

12

Safety Galaxy® 170 R/S CO2 Incubators English (EN)

NOTICE! Risk of material damage

 Never put any liquid material on the top of the incubator. Spilled liquid could cause short circuit; this would cause permanent damage to the incubator. NOTICE! Risk of material damage

 To avoid possible damage to the CO2 sensor, never leave water in the humidity tray while the incubator is switched off, or when a high temperature disinfection cycle is initiated (optional feature).  Allow a clearance of 50 mm (2 in) to allow access for oxygen sensor removal. NOTICE! Risk of material damage

 CO2 gas pressure must not exceed 0.1 MPa (1.0 bar, 14.5 psi) at the CO2 in-line pressure regulator.

 For O2 option: N2 gas pressure must not exceed 0.1 MPa (1 bar, 14.5 psi) at the in-line pressure regulator.

 For O2 option: Remove the O2 sensor when using high temperature disinfection. NOTICE! Risk of material damage

 The instrument or equipment, and its external connections, to be used inside the chamber should be specified as suitable for use in a humid environment, and at 37 °C. If in doubt, consult with the manufacturer of the equipment.  Always ensure the connections are properly and securely made.  Instruments bring heat into the chamber. Do not bring too much heat into the chamber. Too much heat will make trouble in the temperature control; this could cause the loss of sample. NOTICE! Risk of material damage

 Do not modify the device; this could cause the loss of sample.

2.6 Symbol

Warning signs on the device Meaning Hazard point

Hot surface

Operating manual must be read

Location

Product description Galaxy® 170 R/S CO2 Incubators English (EN)

3 3.1

Product description Product overview

3.1.1

Galaxy 170 R CO2 Incubators

Abb. 3-1: Front and rear view of the 170 R CO2 Incubator

1

8 Fig. 3-1:

2

3

7

7

4

6

5

Front and rear view of the 170 R CO2 Incubator

1 CO2 sample port

5 O2 sensor location

2 Display/Interface

6 Left-hand side of control box

3 Inline pressure regulator location

7 Holes for installing the lifting handles

4 Access port

8 Right-hand side of control box

13

14

Product description Galaxy® 170 R/S CO2 Incubators English (EN)

Abb. 3-2: Left-hand side control box

2

1 2 3 3

Fig. 3-2:

Left-hand side control box

1 Auto-Zero filter 2 Inline pressure regulator CO2

3 Inline pressure regulator N2 (option)

Product description Galaxy® 170 R/S CO2 Incubators English (EN)

Abb. 3-3: Right-hand side control box

1 2

3 4

Fig. 3-3:

Right-hand side control box

1 BMS relay contact alarm socket

3 On/Off switch

2 RS232 socket

4 Mains/power cord receptacle

15

16

Product description Galaxy® 170 R/S CO2 Incubators English (EN)

3.1.2

Galaxy 170 S CO2 Incubators

Abb. 3-4: Front and rear view of the 170 S CO2 Incubator

1

7 Fig. 3-4:

2

3

6

6

4

5

Front and rear view of the 170 S CO2 Incubator

1 CO2 sample port

5 Left-hand side of control box

2 Display/Interface

6 Holes for installing the lifting handles

3 Inline pressure regulator location

7 Right-hand side of control box

4 Access port

Product description Galaxy® 170 R/S CO2 Incubators English (EN)

Abb. 3-5: Left-hand side control box

1

CO

2

2 3

Fig. 3-5:

Left-hand side control box

1 Inline pressure regulator CO2 2 On/Off switch

3 Mains/power cord receptacle

17

18

Product description Galaxy® 170 R/S CO2 Incubators English (EN)

Abb. 3-6: Right-hand side control box

1 2 3

Fig. 3-6:

Right-hand side control box

1 RS232 socket 2 BMS relay contact alarm socket

3 Auto-Zero filter

Product description Galaxy® 170 R/S CO2 Incubators English (EN)

3.1.3

Inside the chamber

Abb. 3-7: Internal components

1 23 4 5

6 7

11

8

9

10

Fig. 3-7:

Internal components

1 Sample port

7 CO2

2 N2 inlet (170 R option)

8 O2 sensor (170 R option)

3 Door switch

9 Humidity sensor (170 R option)

4 Not used

10 Water level sensor (170 R option)

5 CO2 inlet

11 Temperature probe

6 Access port

19

20

Product description Galaxy® 170 R/S CO2 Incubators English (EN)

3.2

Delivery package

Quantity

Description

1

Operating manual

4

Non-tip perforated shelves Installed

2

Wired shelf racks Installed

1

Humidity tray Installed

1 2

White porous CO2 sensor cover Installed Packed in accessories bag

1

Colored protective cover Packed in accessories bag

1

Mains/power cord Packed in box inside outer carton

1

10 mm (0.4 in) outer diameter tubing with 6.5 mm (0.2 in) inner diameter with large white filter Packed in accessories bag

4

Screw-in lifting handles Packed in accessories bag

1

Auto-zero CO2 inlet filter Installed

3

Spare shelf rack foot Packed in accessories bag

3

Spare shelf rack spacer Packed in accessories bag

4

Silicone rubber suction feet Packed in accessories bag

1

Safety fastening kit

1

BMS standard 6-pin DIN plug Packed in accessories bag

2

Plug for access port Installed

1

Plug for O2 sensor Installed

1

Jaw wrench for adjusting the feet Packed in accessories bag

4

Plugs for threads for stacking stand assembly Installed