ECLIPSE 80i Instructions March 2012

Introduction Thank you for purchasing this Nikon product. This instruction manual, which describes basic microscope operations, is intended for users of the Nikon ECLIPSE 80i microscope. To ensure correct use, please read this manual carefully before operating the product. • This manual may not be reproduced or transmitted in whole or in part without Nikon's express consent. • The contents of this manual are subject to change without notice. • Although every effort has been made to ensure the accuracy of this manual, errors or inconsistencies may remain. If you note any points that are unclear or incorrect, please contact your nearest Nikon representative. • Some of the products described in this manual may not be included in the set you have purchased. • Make sure you have read the manuals for any other products attached to or to be used with this microscope (super high-pressure mercury lamp power supply, high-intensity light source, etc.).

Warning/Caution symbols used in this manual Although Nikon products are designed to provide the utmost safety, ignoring safety precautions or improper use may result in personal injury or property damage, as well as voiding the terms of the warranty. To ensure safe use, please read the instruction manual carefully and thoroughly before trying to operate the instrument. Do not discard this manual. Store in a convenient location near the product for ready reference. In this manual, safety precautions are indicated by the following symbols. For safe, correct use of the microscope, always follow the instructions indicated by these symbols.

Symbol

Meaning

WARNING

Disregarding instructions indicated by this symbol may result in death or serious injury.

CAUTION

Disregarding instructions indicated by this symbol may result in injury or property damage.

1

Introduction

Meaning of symbols used on the product When appearing on the product, the symbols below indicate the need for caution at all times during use. Consult the instruction manual and read the relevant instructions before attempting to use or adjust any part to which the symbol has been affixed. Caution! Biohazard This symbol found on the stage indicates the following:0 • WARNING: Contact between sample and microscope may result in biohazard risks. • To avoid biohazard contamination, avoid touching the contaminated portion with bare hands.

• Decontaminate the contaminated part according to the standard procedure specified for your laboratory. Caution for heat This symbol found on the lamphouse of the ECLIPSE 80i indicates the following: • The lamp and surrounding areas (including the lamphouse) become very hot during and immediately after a period of illumination. • Risk of burns. Do not touch the lamp or surrounding areas during or immediately after a period of illumination.

• Make sure the lamp and surrounding areas have cooled sufficiently before attempting to replace the lamp

2

Safety Precautions Please follow the safety precautions given below.

WARNING 1.

Intended use of the product This product is intended primarily for microscopic observations of cells and tissue set on glass sides using diascopic (transmitted) and episcopic illumination. It is intended for use in experimentation and observation of cells and tissue in the fields of pathology and cytology in hospital and other laboratory settings.

2.

Do not disassemble. Disassembly may result in malfunctions and/or electrical shock and will void the terms of the warranty. Never attempt to disassemble any part other than the parts described in this manual. If you experience problems with the product, contact your nearest Nikon representative.

3.

Read the instruction manuals carefully. To ensure safety, carefully read this manual and the manual provided with any other equipment used with this product. Observe all warnings and cautions given at the beginning of each manual. When the digital imaging head or the D-FL Epi-illumination attachment is attached to the microscope The mercury lamp (or xenon lamp) for Epi-fl microscopy requires special care during handling. Make sure you have read the instruction manual for the light source (super high-pressure mercury lamp power supply or high-intensity source).

4.

Power cord for microscope and the power cord for AC adapter Use one of the power cords specified. Using the wrong power cord may result in fire or other hazards. The product is classified as subject to Class I protection against electrical shock. Make sure it is connected to an appropriate ground terminal. Refer to Chapter 8 for the power cords specified. To prevent electric shock, always turn off the main power switch (press it to the " " position) of the product before attaching or detaching the power cord.

5.

Inspect the AC adapter (when using the D-CB C-BOX). The D-CB C-BOX is powered by an AC adapter. Use only the specified adapter model meeting the requirements. Using any other type of adapter may result in malfunction, overheating, and/or fire. Refer to Chapter 8 for the adapter specified. • To prevent malfunctions and/or fire, use the AC adapter in a well-ventilated location. To ensure proper heat radiation and to prevent overheating, never cover or place any object on the adapter. • To prevent malfunctions, always turn off the power switch (the switch is in the out position) of the C-BOX before attaching the AC adapter.

3

Safety Precautions

WARNING 6.

Heat from the light source The lamp and surrounding areas (including the lamphouse) will become very hot during and immediately after a period of illumination. • Risk of burns. Never touch the lamp or surrounding areas during or immediately after a period of illumination. • Make sure the lamp and surrounding areas have cooled sufficiently before attempting to replace the lamp • To avoid risk of fire, do not place fabric, paper or highly flammable volatile materials such as gasoline, petroleum benzine, paint thinner or alcohol near the lamphouse while the lamp is lit or during a period of around thirty minutes after the lamp has been turned off.

7.

Mercury lamps and xenon lamps (when the digital imaging head or the D-FL Epi-illumination attachment is attached) The mercury lamp (or xenon lamp) for the digital imaging head or the D-FL Epi-illumination attachment requires special care during handling. For safe and correct use of this system, carefully read the warnings below. Keep in mind all potential hazards. Additionally, carefully read the manual for the super high-pressure mercury lamp power supply (or high-intensity light source) and the manual (if provided) from the lamp manufacturer, then follow the instructions given therein. Hazards of Mercury Lamps and Xenon Lamps 1)

When lit, mercury (and xenon) lamps radiate ultraviolet light that can damage your eyes and skin. Direct viewing of the light may result in blindness.

2)

The lamps contain sealed gas under very high pressure, pressure that increases when the lamp is on. If the lamp is scratched, fouled, subjected to high external pressure or physical impact, or used beyond its service life, the sealed gas may escape or the lamp may burst, resulting in gas inhalation, injury from glass, or other injury.

3)

When the lamp is lit, the lamp and surroundings will become extremely hot. Touching the lamp with bare hands may result in burns; flammable materials placed near the lamp may ignite.

4)

Using the wrong lamp type may result in accidents, including bursting of the lamp.

Safety is a top design priority for Nikon products. The preceding hazards should pose no danger as long as the user observes all of the warnings and cautions given in the manuals, and uses the system only for its intended purpose. However, failure to heed the warnings and cautions given in the manuals, subjecting the system to shock or impact, or attempting to disassemble the system may result in accidents and injury. Make sure you are familiar with and adhere to all warnings and cautions. 8.

Always turn off the lamp when changing filter cubes (when the digital imaging head or the D-FL Epi-illumination attachment is attached to the microscope). When changing filter cubes, always turn off the digital imaging head or the D-FL Epi-illumination attachment. Leaving the lamp on may result in ultraviolet exposure.

9.

Leave the D/UV slider in the optical path (when using the digital imaging head or the D-FL Epi-illumination attachment). Always leave the D/UV slider (which is one of the D-ES EPI ND slider types) in the optical path when performing Epi-bright-field microscopy, Epi-dark-field microscopy, or Epi-DIC microscopy with the mercury lamphouse attached. If the slider is moved out of the path, ultraviolet light will be radiated and damage your eyes.

4

Safety Precautions

WARNING 10. Hazardous Sample This microscope is intended primarily for microscopic observations of cells and tissue set on glass sides. Check to determine whether a sample is hazardous before handling. Handle hazardous samples according to the standard procedure specified by your laboratory. If the sample is potentially infectious, wear rubber gloves and avoid touching samples. If contact occurs between a sample and the microscope, decontaminate the contaminated portion according to the standard procedure specified for your laboratory.

CAUTION 1.

Turn off power during assembly, connection/disconnection of cords, lamp replacement, and maintenance. To prevent electric shock and/or malfunctions, always turn off the power switch(es) of the product (press it (them) to the "!" position) and unplug the power cord from the wall outlet before assembly, connecting or disconnecting of cords, lamp replacement, and cleaning of the microscope and the objective.

2.

Lamp replacement precautions To avoid burns, wait at least 30 minutes after the lamp is turned off to give it sufficient time to cool. To avoid electric shock or malfunctions, never attempt to replace the lamp without first turning off the power switches for the microscope and the peripheral devices (press them to the "!" position) and unplugging the power cord from the wall outlet. Make sure the lamphouse cover is securely fitted to the lamphouse after lamp replacement. Never turn on the lamp while the lamphouse cover is open. Do not break up used lamps; instead, dispose of them as special industrial waste or as specified by local regulations.

3.

Use the specified lamp and lamphouse. Always use the specified lamp and lamphouse. Using an unspecified lamp may cause malfunction or fire. Refer to Chapter 8 for the specified lamp and lamphouse.

4.

Avoid contact with water. Never allow water to come into contact with the product, and keep the product away from liquids. Splashing water onto the product may cause a short, resulting in malfunction or abnormal heating. If water is splashed onto the product, immediately turn off the power switch (press it to the "!" position) and remove the power cord from the receptacle. Then wipe off moisture with a dry cloth or something similar. If water enters the product, do not use; in this case, contact your distributor.

5.

Do not place any object on top of the product. Do not place any object on top of the product or cover it with a cloth or the like. The system temperature will rise, resulting in malfunctions.

5

Safety Precautions

CAUTION 6.

Cautions on assembling and installing the microscope • Take care to avoid pinching your fingers or hands during microscope assembly. • Scratches or fouling such as fingerprints on optical components (such as lens and filters) will degrade microscope images. Be careful to avoid scratches or direct contact with the lens and filters. • The main unit weighs about 12 kg. Grasp the main unit by the recesses at the front of the arm and at the bottom of the main unit. • Remove all attachments (if attached) from the microscope before carrying the microscope. • Do not install the product in a locker or cabinet.

7.

Remove any covers from the product before switching on. Do not use the product while covered with a cloth, etc., as this will give rise to abnormal heat, which could cause a fire.

8.

Caution concerning long, sustained observations To relieve fatigue resulting from long observation sessions, limit continuous observations to one hour. Take at least 10- to 15-minute breaks between observation sessions. Adjust the layout of other equipment (such as the display and the mouse) and match to the position of the product and the height of your chair.

9.

Disposal of the product To avoid biohazard risks, dispose of the product as contaminated equipment according to the standard procedure specified for your laboratory.

6

Notes on handling the product

Notes on handling the product 1.

Handle the product gently. This product is a precision optical instrument and requires gentle handling. Avoid subjecting it to sudden impact and shocks. Even relatively minor impacts are capable of affecting the precision of the object.

2.

Weak electromagnetic waves The product emits weak electromagnetic waves. Do not place precision electronic devices near the product to avoid degrading their performance. If TV or radio reception is affected, move the TV or radio farther away from the product.

3.

Scratches, dirt, and foreign particles on the lens Scratches or fouling such as fingerprints on optical components (such as lens and filters) will degrade microscope images. If these parts become dirty, clean them as described in chapter "7. Care and maintenance" at the end of this manual.

4.

Dirt on the lamps Never touch the lamp with bare hands. Dirt or fingerprints on the lamp will result in uneven illumination and reduce the service life of the lamp. Always wear gloves when handling lamps.

5.

Installation location This product is a precision instrument. Use or storage in inappropriate environments may result in malfunctions or poor performance. Consider the following factors when selecting an installation location: • Select a vibration-free location. Install the product on a level surface. • Install the product at least 10 cm away from walls. • Choose a location less exposed to hazards in the event of collisions, earthquakes, or other potential disasters. To keep the product from falling, use strong rope or other means if necessary to secure it to the working desk or to another heavy, stable item. To fix the microscope, there are two M6 screw holes (one at the right and the other at the left) behind the recess at the base of the main body (back side). • Avoid locations exposed to direct sunlight, locations immediately under room lights, and other bright locations. • Avoid locations with excessive dust. • Do not install the product near any liquid. • Make sure the ambient temperature is 0 to 40°C and humidity is 85% or less. Installing the 80i in hot, humid locations may result in mildew formation or condensation, impairing performance or generating malfunctions. • Do not install the product in a locker or cabinet. • Select a layout that allows easy removal of the power cord from the product's AC inlet in the event of an emergency. • Room lights just above the microscope may reduce visibility in the objective as extraneous light. If possible, switch off room lights directly above the microscope when making observations.

7

Notes on handling the product

6.

Focusing knobs • Never turn the focus knobs on the left and right sides of the microscope in opposite directions at the same time. Doing so may damage the microscope. • Turning the coarse focus knob past its farthest point will damage the microscope. Never use undue force when turning the knob.

7.

Protect the ports from dust and extraneous light (when the trinocular eyepiece tube, the digital imaging head, or the C-TE ergonomic binocular tube is attached). To keep out extraneous light and dust, always attach the supplied cap to any port not currently in use.

8.

Handling of filters (when the digital imaging head or the D-FL Epi-illumination attachment is attached to the microscope) • Interference filters (especially excitation filters, which are exposed to strong light) degrade over time. Replace them after the appropriate hours. • Filter characteristics may alter if the filter is exposed to high humidity. To prevent changes in or degradation of filter characteristics, avoid using or storing the filters under conditions of high humidity or high temperature. Avoid subjecting filters to rapid temperature changes. When a filter is not in use, store in a desiccator or hermetically sealed container with a drying agent. • The filters in the nine types of filter cubes listed below offer sharp, high-resolution waveform characteristics superior to normal filters. However, due to their sophisticated coatings, they must be handled with special care. In particular, take care to avoid abrasion from cleaning. (Follow the procedure described in section "1. Filter and lens cleaning" of chapter "7. Cleaning and Maintenance.") Single-band filter cubes: DAPI, FITC, TxRed, GFP Multi-band filter cubes: F-R, F-T, D-F, D-F-R, D-F-T

9.

Handling of the D-FLD dark-field illumination cube (when using the digital imaging head or the D-FL Epi-illumination attachment) There are two wings in front of the D-FL Epi-illumination attachment, these prevent light leakage. Do not apply any pressure onto the wings, as malfunction may result.

10. Unpacking and unclamping 1)

Check the contents to ensure that the package contains the following: • ECLIPSE 80i

x1

• Tools:

Hex wrench x2 Allen key x1

• Accessories: Shield cover x1 Dust-proof cover

x1

2)

Before connecting the power cord, remove the cushioning material from under the sub-stage by turning the focusing handle and raising the sub-stage.

3)

Do not discard the packing case, as it may be needed for future transportation.

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Abbreviations Used in The Manual

Abbreviations Used in The Manual The product names and abbreviations used in this manual are given below. The manual uses the following abbreviations:

Name of device

Abbreviation

Microscope ECLIPSE 80i

80i

C-ER Eye Level Riser

Eye Level Riser

C-TE Ergonomic Binocular Tube

Ergonomic Binocular Tube

C-TEP DSC Port for Ergonomic Binocular Tube

DSC Port

D-FL Epi-illumination Attachment

Epi-illumination Attachment

D-DH Digital Imaging Head M

DIH-M

D-CB C-Box

C-Box

D-LH Precentered Lamphouse

Lamphouse

D-NID6 Intelligent Sextuple DIC Nosepiece

Intelligent Nosepiece

C-HS Hand Switch

Hand Switch

DS Camera Head DS-5M

Camera Head

DS Camera Control Unit DS-L1

DS-L1

DS Camera Cable

Camera Cable

Super High-pressure Mercury Lamp Power Supply Unit

Mercury Lamp Power Supply Unit

Super High-pressure Mercury Lamphouse

Mercury Lamphouse

9

How to use this instruction manual

How to use this instruction manual This instruction manual is composed of two parts, as below: Manual 1 "Microscopy" describes basic microscope operations that you must follow. Please read this manual carefully before operating the product. Manual 2 "Reference" describes the operations of each attachment. Please read an appropriate section as necessary.

Manual 1

Title

Importance

Content

Microscopy

Must be read

Safety precautions Microscopy, Assembly, Troubleshooting, Cleaning and maintenance, Specifications

Manual 2

Reference

As necessary

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Detailed operations of attachments

Using Microscopy Manual

Using Microscopy Manual The part names and microscopy procedures are described in three separate sections, corresponding to specific configurations of 80i and accessory devices. Confirm the combination of the 80i and accessory devices you are using, then refer to the appropriate section.

Combination

Description

Bright-field set

See Chapter 1.

(Eyepiece tube mounted directly on 80i)

(For operating procedures and detailed information on individual parts, refer to the separate Reference manual.)

(Camera and monitor are optional.)

See Chapter 2.

80i + Epi-illumination attachment

(For operating procedures and detailed information on individual parts, refer to the Reference manual.)

(Camera and monitor are optional.)

80i + DIH-M

See Chapter 3.

(Camera and monitor are optional.)

(For operating procedures and detailed information on individual parts, refer to the Reference manual.)

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Contents

Contents Introduction ... 1 Warning/Caution symbols used in this manual ... 2 Meaning of symbols used on the product ... 2

Safety Precautions ... 3 WARNING... 3 CAUTION... 5 Notes on handling the product ... 7 Abbreviations Used in The Manual ... 9 How to use this instruction manual ...10 Using Microscopy Manual ...11 Chapter 1 Part Names and Microscopy Procedures (Bright-Field Set) ...14 1.1

Names of Main Components ... 14

1.2

Names of Parts Used to Make Adjustments... 15 1.2.1 Right view ... 15 1.2.2 Left view ... 16 1.2.3 Rear view... 16 1.2.4 Ergonomic binocular tube with camera attached... 17

1.3

Bright-Field Microscopy ... 18

1.4

Photomicroscopy... 22

Chapter 2 Part Names and Microscopy Procedures (With Epi-illumination Attachment Mounted) ...24 2.1 2.2

Names of Main Components ... 24 Names of Parts Used to Make Adjustments... 25 2.2.1 Right view ... 25 2.2.2 Left view ... 26 2.2.3 Rear view (without accessory device) ... 26

2.3

Bright-Field Microscopy ... 27

2.4

Epi-fluorescence Microscopy ... 31

2.5

Photomicroscopy... 35

Chapter 3 Part Names and Microscopy Procedures(With DIH-M Mounted)) ...37 3.1

Names of Main Components ... 37

3.2

Names of Parts Used to Make Adjustments... 38 3.2.1 Right view ... 38 3.2.2 Left view ... 39 3.2.3 Rear view (without accessory device) ... 40

3.3

Bright-Field Microscopy ... 41

3.4

Epi-fluorescence Microscopy ... 45

3.5

Photomicroscopy... 49

Chapter 4 Assembly ...51 Chapter 5 Replacing Consumables ...63 5.1

Replacing the lamp... 63

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Contents

Chapter 6 Troubleshooting...64 6.1

Optical... 64

6.2

Electrical ... 65

Chapter 7 Cleaning and Maintenance ...66 7.1

Lens cleaning ... 66

7.2

Cleaning the product ... 66

7.3

Disinfecting the product ... 66

7.4

Storage... 67

7.5

Periodic Inspections (fee charged)... 67

Chapter 8 Specifications...68 8.1

Specifications ... 68

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1 Part Names and Microscopy Procedures (Bright-Field Set) 1.1

Names of Main Components

Eyepiece

Eyepiece tube

Camera head (Required for image capture) (One example shown in the photo) DSC port (Required for image capture) Revolving nosepiece Objective Lamphouse Specimen holder Stage Condenser

Microscope (main body) Camera control unit (Required for image capture) (One example shown in the photo)

14

Chapter 1

Part Names and Microscopy Procedures (Bright-Field Set) 1.2

Names of Parts Used to Make Adjustments

1 1.2

Names of Parts Used to Make Adjustments

1.2.1

Right view

Diopter adjustment ring

Optical path switching lever

Aperture diaphragm lever

Y stage knob X stage knob (Provided on the left side in certain products) Filter IN/OUT switches

Field diaphragm knob

Fine focus knob

Coarse focus upper-limit clamp

15

Chapter 1

Part Names and Microscopy Procedures (Bright-Field Set) 1.2

1.2.2

Names of Parts Used to Make Adjustments

Left view

Condenser focus knob

(Provided on the right side in certain products)

Coarse focus torque adjustment knob Coarse focus knob Condenser centering screw

Fine focus knob

Brightness control knob

1.2.3

Preset switch

Power ON indicator

Rear view

Tool storage compartment

Power switch AC power inlet

Input voltage indication

Lamphouse

16

Chapter 1

Part Names and Microscopy Procedures (Bright-Field Set) 1.2

Names of Parts Used to Make Adjustments

1 1.2.4

Ergonomic binocular tube with camera attached

Camera cable connector Camera head

C mount Camera fine focus adjustment screw Camera centering screws

17

Chapter 1

Part Names and Microscopy Procedures (Bright-Field Set) 1.3

1.3

1

Bright-Field Microscopy

Bright-Field Microscopy Turn on power.

Press the power switch to the "|" position.

2

Press the preset switch.

Press the preset switch.

3

Move the ND8, ND32, and NCB11 filters into the optical path.

NCB11 ND32 ND8

4

Push in the optical path switching lever to direct the full optical path toward the binocular section.

18

The full optical path directed toward the binocular section

Push in the optical path-switching lever.

Chapter 1

Part Names and Microscopy Procedures (Bright-Field Set) 1.3

Bright-Field Microscopy

1

5

Raise the condenser to the uppermost position.

Raise the condenser using the condenser focus knob.

6

Fully open the field diaphragm and aperture diaphragm. (When using the 1-100x condenser, move the top lens into the optical path.)

Fully open the aperture diaphragm using the aperture diaphragm lever.

7

Fully open the field diaphragm using the field diaphragm knob.

Set the 10x objective into the optical path.

Select the 10x objective.

8

Set a specimen and move the portion to be viewed into the optical path.

Set a specimen and secure in place using the specimen holder.

Move the portion to be viewed into the optical path using the XY stage knobs.

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