ECLIPSE Ni-U Instruction Manual and Assembly/ Maintenance Manual

Introduction

Introduction Thank you for purchasing a Nikon product. This instruction manual is written for users of the Nikon ECLIPSE Ni-U microscope. To ensure correct usage, read this manual carefully before operating this product. ● No part of this manual may be reproduced or transmitted in any form without prior written permission from Nikon. ● The contents of this manual are subject to change without notice. ● The equipment described in this manual may differ from the actual product in its appearance. ● Although every effort has been made to ensure the accuracy of this manual, errors or inconsistencies may remain. If you note any points that are unclear or incorrect, please contact your nearest Nikon representative. ● Some of the equipment described in this manual may not be included in the set you have purchased. ● If you intend to use any other equipment with this product, read the manual for that equipment too. ● If this equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. ● Training: This product can be used without special training, provided that this manual is read thoroughly before use. Kindly contact your nearest Nikon representative if you have any questions, find any errors, or wish to provide us with your opinion.

i

Introduction

Contents of the Manual The instruction manual for ECLIPSE Ni-U is provided as two volumes.  Operation Safety Precautions Components Microscopy Procedures Operation Flowchart Bright/Dark-field Microscopy Epi-fluorescence Microscopy Differential Interference Contrast Microscopy Phase Contrast Microscopy Motorized Bright-field Microscopy Motorized Epi-fluorescence Microscopy Individual Operations

 Assembly/Maintenance (This manual) Assembly Troubleshooting Maintenance and Storage Specifications Before reading the “Assembly/Maintenance” manual, read the “Safety Precautions” in the “Operation” manual.

Symbols Used in This Manual The following symbols are used in this manual.  Symbols for Safety

WARNING CAUTION

Highlights important information that should be noted for safety. Read “Safety Precautions” for details.

 Other Symbols Indicates information you should note or comply with to prevent defects or malfunction of this product.

Indicates information you should be aware of in using this product, as well as other useful information.

ii

Summary of Contents

Summary of Contents (See the next page for the detailed contents.) Introduction Contents of the Manual Symbols Used in This Manual

iii

Specifications

Specifications

Maintenance and Storage

Maintenance and Storage

Troubleshooting

Troubleshooting

Assembly

Assembly

Contents

Contents Introduction ... i Contents of the Manual ...ii Symbols Used in This Manual ...ii

Summary of Contents ... iii

Assembly... 1 1

ECLIPSE Ni-U System Configuration ...2

2

Components List ...3

3

Assembly Method...4 Introduction ... 4 [1] Check the input voltage... 4 [2] Attach the DIA motorized shutter (optional). ... 5 [3] Attach the lamp. ... 5 [4] Attach the lamphouse for dia-illumination. ... 5 [5] Attach the standard arm/contact arm. ... 6 [6] Attach the epi-fluorescence cube turret and epi-fluorescence attachment (required for epi-fluorescence microscopy)... 7 ■ Attaching a filter cube ... 8 ■ Replacing excitation and barrier filters ... 9 ■ Attaching a light shielding plate ... 9 [7] Attach the quadrocular tilting tube and DSC zooming port for quadrocular tube. ... 10 [8] Attach the EPI motorized shutter (optional). ... 10 [9] Attach eyepieces. ...11 [10] Attach the substage...11 [11] Attach the condenser holder. ... 12 [12] Attach the stage. ... 12 [12.1] For standard stage ... 12 [12.2] For rotatable ceramic coated stage... 12 [13] Attach the condenser... 13 ■ Attaching the optical module to the universal condenser... 13 ■ Attaching the DIC module to DIC condenser oil... 17 [14] Attach the nosepiece... 19 [15] Attach the objective. ... 19 [16] Attach the DIC rotation polarizer unit (required for differential interference contrast microscopy). ... 19 [17] Attach the eyelevel riser (optional)... 20 [18] Attach a camera and connect the DS camera control unit (optional). ... 20 [18.1] When attaching to the DSC port for the ergonomic binocular tube 20 [18.2] When attaching to the DSC zooming port for quadrocular tube ... 21 [18.3] When attaching to the quadrocular tilting tube... 21 [18.4] When attaching a camera to the trinocular tube ... 21 [19] Replace the ND filter (optional). ... 22 [20] Connect the motorized device cable... 22 [21] Connect the power cord. ... 24

Troubleshooting... 25 1

Optical System and Operation ...26 1.1 1.2 1.3

General... 26 Epi-fluorescence Microscopy ... 30 Differential Interference Contrast Microscopy... 31

iv

Contents 1.4

2

Phase Contrast Microscopy ... 32

Electrical Requirements ...33 2.1 2.2

General... 33 Epi-fluorescence Microscopy ... 34

Maintenance and Storage ... 35 1

Replacing the Lamp ...36

2

Cleaning ...37 2.1 2.2 2.3 2.4

Cleaning Lenses... 37 Cleaning Parts Other than the Lens... 37 Cleaning Immersion Oil... 38 Decontaminating the Product... 38

3

Transportation ...38

4

Storage...38

5

Regular Inspections (Charged) ...38

Specifications ... 39 1

Microscopy (Principles) ...40

2

Performance Properties ...40

3

Physical Properties ...43

v

Contents

vi

1

Assembly

Chapter

1

Assembly This chapter contains an Ni-U system configuration diagram, a list of its components, and explains how to assemble the system. Read appropriate notes such as CAUTION “9 Cautions on assembling and installing the product” at the beginning of the separately provided instruction manual “Operation” or “4 Installation location” in “Notes on Handling the Product” in the same manual before you work on assembling. ■ Assembly tools provided with the microscope • Hex driver x3: 2 mm across flats x3 • Hex wrench x2: 3 and 4 mm across flats

1

Chapter 1

1

Assembly

ECLIPSE Ni-U System Configuration ENG mount camera C mount camera Photomicrography device

Camera cable to DS-U3 or DS-L3

Various adapters DS camera head

Assembly

CFI eyepiece

Centering telescope

DSC port for ergonomic binocular tube

TV adapter

Tube adapter for quadrocular tube

Motorized shutter cable to control box B

Trinocular tube

Binocular tube

Quadrocular tilting tube

Ergonomic tube

DSC zooming port Motorized shutter Motorized shutter adapter

To control box B

Eyelevel riser

Filter cube

Epi-fluorescence cube turret*2

Analyzer unit

Contact arm

Epi-fluorescence attachment

HG adapter

Standard arm D-FB

FL/DIC analyzer

Excitation balancer

HG fiber

Analyzer slider

Lambda plate

Mercury lamp DIC nosepiece*1

DIC slider

Nosepiece

Nosepiece with analyzer slot*1

RS-232C cable to control box B for motorized operation

CFI objective

Motorized shutter cable to control box B

Ni-U main body

HG precentered fiber illuminator

Substage for rotatable ceramic coated stage

Rotatable ceramic coated stage

To Ni-U main body Motorized shutter

Substage

Motorized shutter adapter

Control box B

Lamphouse

Stage

EPI

1.4

1.2

Achr-Apl N.A=1.4 1.0 0.8 0.6 0.4

FL

INTSL

DIA

0.2

SHUTTER OPEN 32

16

8

4

2

1

INTSL ND

Condenser

Condenser holder

OBJ

FL

CONTROL NI-SRCP MADE IN JAPAN

Polarizer unit

Simple remote control pad

*1: A contact arm is required to use a motorized/intelligent nosepiece. *2: A contact arm is required to use a motorized/intelligent epi-fluorescence cube turret. *3: DS-U3 DS camera control unit can not be connected via USB.

2

DS-L3/ DS-U3 Camera Control Unit

PC USB cable to control box B*3

Camera trigger cable to Ni-U main body or control box B

Chapter 1

2

Assembly

Components List

The ECLIPSE Ni-U components are shown in the table below. Depending on when you purchased the ECLIPSE Ni-U, there may be devices not yet available for use and devices not listed that are already available for use. Contact your nearest Nikon representative for details.

Illuminator for dia-illumination Arm

Tube

DSC port Eyepiece

Nosepiece

Objective Substage

Stage

Condenser holder

Condenser

Epi-fluorescence cube turret Epi-fluorescence attachment Mercury lamp illuminator

Analyzer unit/ Slider

Polarizer unit

Shutter

Device Name ECLIPSE Ni-U (12V 100W 100-240V power supply for illumination integrated) Precentered lamphouse (12V 100W) Halogen lamp (100W) Standard arm Contact arm Binocular tube Trinocular eyepiece tube F Trinocular eyepiece tube T Ergonomic tube Quadrocular tilting tube DSC port for ergonomic binocular tube DSC zooming port for quadrocular tube CFI eyepiece Centering telescope Universal quintuple nosepiece BD quintuple nosepiece Sextuple nosepiece Sextuple nosepiece with analyzer slot Sextuple DIC nosepiece Intelligent DIC sextuple nosepiece Intelligent Septuple nosepiece Motorized DIC sextuple nosepiece Motorized septuple nosepiece CFI objective Substage Substage for rotatable ceramic coated stage Right handle stage with 2S holder Left handle stage with 2S holder Right handle ceramic coated stage with 1S holder Left handle ceramic coated stage with 1S holder NI-U right handle rotatable ceramic coated stage with holder NI-U left handle rotatable ceramic coated stage with holder Condenser holder Dark field condenser (Oil) and (Dry) C-C aplanatic condenser C-C achromat condenser No.9 C-C abbe condenser No.9 X LWD condenser C-C slide achro condenser 2-100x C-C achromat swing-out condenser 1-100x C-C phase turret condenser DIC condenser (Oil)

DSC retention support

Remarks

NI-LH PHILIPS 7724 OSRAM HLX 64623 NI-SAM NIU-CAM C-TB C-TF C-TT C-TE2 NI-TT C-TEP2 NI-RPZ CFI CFI UW C-CT L-NU5 L-NBD5 C-N C-NA D-ND6 NI-ND6-I NI-N7-I NI-ND6-E NI-N7-E CFI LU Plan Fluor Epi LU Plan Fluor BD NI-SS NI-SSR C-SR2S C-SL2S C-CSR1S C-CSL1S

Combine with a manual model such as manual nosepiece Combine with a motorized models such as intelligent/motorized nosepiece C-TBM mildew-proof type also available C-TFM mildew-proof type also available

Connected to Nikon DS camera head or other C mount camera. Nikon DS camera head is controlled from DS-L3/DS-U3.

LU Plan Fluor Epi/BD can be attached to the L-NU5 or L-NBD5 nosepiece. LU nosepiece adapter is required to attach a non-BD objective to L-NBD5. Rotatable ceramic coated stage can not be attached

NIU-CSRR2 NIU-CSLR2 NI-CH

Cannot be used when rotatable ceramic coated stage is attached. Used with OIL DIC module Supports DRY DIC module, PH module, dark field module, NI-CALN1 2-4x auxiliary lens, D-C 2-4x auxiliary lens

D-CUO

Universal condenser (Dry)

NI-CUD

Cube turret Intelligent epi-fluorescence cube turret Motorized epi-fluorescence cube turret

NI-FLT6 NI-FLT6-I NI-FLT6-E

A filter cube and NI-FA FL/DIC analyzer attachable. D-FB excitation balancer attachable.

Epi-fluorescence attachment

NI-FLEI

HG precentered fiber illuminator Motorized HG precentered fiber illuminator

C-HGFI C-HGFIE

Analyzer slider for DIC

D-DA

Analyzer tube for simple polarization Analyzer tube for first-order red compensation Analyzer slider for simple polarization Analyzer slider for first-order red compensation DIC slider Lambda plate DIC rotatable polarizer unit Polarizer unit for simple polarization Polarizer unit for first-order red compensation

C-ISA C-IA D-SA C-AS D-C D-LP D-DP C-SP C-TP

Motorized shutter

NI-SH-E

Motorized shutter adapter for upright epi-fl Motorized shutter adapter for dia-illumination Control box B Simple remote control pad

NI-SHAEP-U NI-SHADI NI-CTLB NI-SRCP

DS-L3/DS-U3 camera control unit

DS-L3 DS-U3

Eyelevel riser

C-ER

Controller

Eyelevel riser Variable intermediate magnification unit

Model ECLIPSE Ni-U

Variable intermediate magnification unit

Y-IM

DSC support column

NI-RPS NI-RPSAA NI-RPSAB

DSC adapter A and B

Tube adapter for quadrocular tube

Tube adapter for quadrocular tube

C-TAQ

TV adapter

TV adapter

Y-TV Y-TV55

Can be attached to C-NA, D-ND6, NI-ND6-I, NI-N7-I, NI-ND6-E, or NI-N7-E nosepiece. Combined with C-SP polarizer unit. Combined with C-TP polarizer unit.

Can be attached to D-ND6, NI-ND6-I, or NI-ND6-E nosepiece. Can be attached to D-ND6, NI-ND6-I, or NI-ND6-E nosepiece.

For connection, NI-SHCL motorized shutter cable is required and is connected to the control box B.

DS-L3 is connected to the control box B via USB cable DS-L3/DS-U3 is connected to the Ni-U main body or the control box B via C-CTC camera trigger cable L3/U3 DS camera I/F cable is used to connect to the camera

Can be attached to the quadrocular tilting tube. Attach a C mount camera, ENG mount camera or a photomicrography device via various adapters. Y-TV can be attached to C-TF or C-TT trinocular tube. Connect a C mount camera, ENG mount camera, or photomicroscopy device with an adapter. Y-TV55 can be attached to C-TF or C-TT trinocular tube. Connect a C mount camera with C mount adapter 0.55x.

3

Assembly

Device Microscope main body

Chapter 1

3

Assembly

Assembly Method Introduction

Assembly

Ni-U is a manual microscope. Basically, a manual model is attached for each device consisting the microscope. However, for nosepiece, epi-fluorescence cube turret, and HG precentered fiber illuminator, motorized models can be attached. In this case, contact arm, control box B, and simple remote control pad are required. In addition, in order to configure information for motorized devices, DS-L3 DS camera control unit must be connected. Motorized devices on Ni-U can be controlled from DS-L3. Note that when using epi-fluorescence cube turrets by layering, motorized/intelligent epi-fluorescence cube turret can only be used in 1st layer. Assemble the device according to the following procedure.

Precautions for connecting cables Cable connections are required for some motorized devices. Be sure to turn off the power to the microscope and peripheral devices before starting cable connection. Cable connections are described in each assembly step. Nikon recommends connecting cables at the end of the assembly. See “20 Connect the motorized device cable.” for the connector connections. Information setting regarding motorized models Set information of the motorized devices or optical element attached on the microscope setup menu for the DS-L3 DS Camera Control Unit. (See Chapter 3 “19 Operations on DS-L3” - “19.1 Setting Up the Microscope.)

1

Check the input voltage. Check the input voltage indicated on the back of the microscope. Use the microscope only if the indicated input voltage matches the power supply voltage for the area in which the microscope will be used. Input voltage label

WARNING

MODEL ECLIPSE Ni-U NIKON CORPORATION TOKYO, JAPAN

100–240V~ 1.7A 50/60Hz

920001 MADE IN JAPAN

This device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) this device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation. This Class A digital apparatus complies with Canadian ICES-003. Cet appareil numérique de la classe A est confirme à la norme NMB-003 du Canada.

DSC

INSPECTION EQUIPMENT 4N75

If the indicated voltage and the supplied voltage differ, do not attempt to use the microscope. Contact your nearest Nikon representative.

LAMP DC12V 100W LAMP CTRL (BOX B)

Checking the input voltage (Microscope rear view) Unlocking the elevating section Tool: flathead screwdriver After you confirm that the input voltage is correct, loosen and remove the tightening screw at the front of the elevating section.

Tightening screw

NCB ND ND 11 8 32 OUT

POWE R IN

Unlocking the elevating section

4

Chapter 1

2

Assembly

Attach the DIA motorized shutter (optional). The motorized shutter is attached by Nikon. Contact your nearest Nikon representative when the DIA motorized shutter needs to be attached or removed. Assembly

3

Attach the lamp. Tool: Hex wrench (3 mm across flats) Lamp handling precautions Avoid touching the glass surface of the lamp with your bare hands.

Insert the hex wrench and loosen the fixing screw. Fixing screw

(1)

Loosen the lamphouse cover fixing screw and lift up the cover to remove.

(2)

Insert the lamp clamp lever to open the socket pin hole. Attach the lamp while holding down the lever. Put the lamp clamp lever back to its original position.

Lamphouse cover

Designated lamp: PHILIPS7724 or OSRAM HLX64623 (3)

Reattach the cover back to its original position and tighten the lamphouse cover fixing screw.

Removing the lamphouse cover Lamp Lamphouse fixing screw Lamp clamp lever

Attaching the lamp

4

Attach the lamphouse for dia-illumination. Tool: Hex driver (2 mm across flats) (1)

Insert the lamphouse at the rear of the microscope and tighten the fixing screws.

(2)

Connect the dia-illumination lamphouse cable to the LAMP connector on the rear of the main body.

Lamphouse fixing screw

EL MOD40V~ ION

100–2

Ni-U ECL IPSE 01 9 2 0 0 PAN MAD

E IN

JA

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DS C

ION INSPECT NT EQUIPME 4N75

CT RL LAM P (BOX B)

DC

LAMP W 12V 100

CAUT

ION!

-High

Tempera

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NI-L

9520 JAPA

01

N

Securing the dia-illumination lamphouse

5

Chapter 1

5

Assembly

Attach the standard arm/contact arm.

Assembly

The attachment procedure is the same for standard arm and contact arm. To use the motorized accessories, attach the contact arm. The contact arm has a contact. When attaching a motorized device on the arm, remove the contact cover beforehand. Tool: Hex wrench (4 mm across flats) (1)

Place the arm while aligning it with the two positioning pins on the main body and tighten the four fixing screws.

(2)

When using contact arm, connect the cable from the CONTACT ARM1 connector to the CONTACT ARM1 connector on the control box B.

(3)

Connect the cable from the CONTACT ARM2 connector to the CONTACT ARM2 connector on the control box B.

Arm fixing screw

Positioning pin (Standard arm) Contact cover Arm fixing screw

(Contact arm)

Attaching the arm

6

Chapter 1

6

Assembly

Attach the epi-fluorescence cube turret and epi-fluorescence attachment (required for epi-fluorescence microscopy). Tool: Hex driver (2 mm across flats) Hex wrench (3 mm across flats)

(1)

Insert the positioning pin of the epi-fluorescence attachment while aligning it with the positioning hole on the epi-fluorescence cube turret side, and then tighten the connection fixing screws (x2) on the epi-fluorescence cube turret with a hex driver.

(2)

When using a motorized or intelligent epi-fluorescence cube turret, remove the contact cover on the contact arm beforehand. (Contact cover is not removed for manual epi-fluorescence cube turret.)

(3)

Loosen the fixing screw on the front of the arm using a hex driver so that the tip of the screw does not protrude into the connecting section.

(4)

Align the round dovetail and convex contact on the bottom of the epi-fluorescence cube turret with the round dovetail and concave contact of the arm and slide the entire mounted attachment to the rear. (There is no concave contact in manual epi-fluorescence cube turret.)

(5)

Tighten the fixing screw loosened in step (3).

(6)

Tighten the four fixing screws on top of the epi-fluorescence attachment.

(7)

Attach the HG adapter to the bayonet mount at the rear of the epi-fluorescence attachment and connect the HG precentered fiber illuminator. (See the instruction manual provided with the HG precentered fiber illuminator.)

F. STO P A. STO P

EX. ADJ

Connection fixing screw

Connecting epi-fluorescence cube turret and epi-fluorescence attachment

P A. STO P F. STO

1

EX. ADJ

2 2

3

4

5

6

Epi-fluorescence attachment fixing screw

Fixing screw

Attaching and fixing epi-fluorescence cube turret and epi-fluorescence attachment set

Bayonet mount HG adapter F. STO P A. STO

P

EX. ADJ

HG fiber MOD EL 0V~ ORAT CORP NIKON , JAPAN TOKYO

ION

100–24 1.7A z 50/60H

Ni-U ECLI PSE 01 9200 N MADE

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IN JAPA

s: two condition n. d operatio undesire cause

following to the

ON INSPECTI NT EQUIPME 4N75

Attaching the HG adapter

7

Assembly

Epi-fluorescence Positioning hole cube turret Epi-fluorescence attachment

First, connect the epi-fluorescence cube turret and epi-fluorescence attachment and then attach them on the microscope arm.

Chapter 1

Assembly

■ Attaching a filter cube Precautions for attaching and removing the cube Assembly

• Be sure to check that the light source is turned off before attaching or removing the cube. • Be sure to check that the power switch for microscope main body is turned off and then attach the cube by

rotating the cube switchover turret. Also for the motorized epi-fluorescence cube turret, attach it by manually rotating the inside turret. (1)

Pull out the filter cube replacement cover on the front of the epi-fluorescence cube turret to remove it.

(2)

Attach the filter cube to the slot.

(3)

Insert the filter cube nameplate into the slot cover window. Insert into the same address window as the address shown inside the slot.

ND4

ND8

ND16

1 6

1

2

3

4

5

A

5 λ

1

6

Filter cube replacement slot cover

1

3 2

5

A

UV-2A

Address indication

EX 330DM 400 380 BA 420

Filter cube

(4)

Cube switchover turret

Turn the cube switchover turret to attach the filter cubes in the remaining slots and also insert the nameplates. For bright-field microscopy For bright-field microscopy, be sure to make the address 1 empty. For motorized/intelligent epi-fluorescence cube turret, only address 1 can be set to [OPEN].

(5)

A λ

6

2

Attaching a filter cube

Restore the slot cover back to its original position.

8

Address indication Nameplate window

Chapter 1

Assembly

■ Replacing excitation and barrier filters The excitation filter, barrier filter, and dichroic mirror can be removed from the cube for replacement.

Turn approx. 30 degrees to secure in place.

Align the projection on the barrier filter with the groove on the filter cube and turn clockwise by approximately 30 degrees to secure it in place. Orientation of the filter •

The filter is two-faced. Be aware that the filter does not face a wrong side. Nikon filters have an arrow indication printed on the outer frame. Make sure that the arrow faces the direction of a dichroic mirror when attaching the filter.

•

Contact your nearest Nikon representative when the dichroic mirror needs to be replaced.

UV-2A

EX 33 DM 400-380 BA 42 0 0

Barrier filter

Excitation filter

Replacing the excitation and barrier filters

■ Attaching a light shielding plate Tool: Hex driver (2 mm across flats) Lightly screw the two fixing screws provided with the light shielding plate into the front bottom of the epi-fluorescence cube turret. Hook the convex part of the light shielding plate to the two fixing screws and tighten the fixing screws.

1 2 2

3

4

5

6

Fixing screw (At the bottom of the epi-fluorescence cube turret)

Fixing the light shielding plate

9

Assembly

Excitation filters are screw-in filters, while barrier filters are slide-in filters.

Chapter 1

Assembly

7 Attach the quadrocular tilting tube and DSC zooming port for quadrocular tube. Assembly

Tools: Hex driver (2 mm across flats) Hex driver (2.5 mm across flats, provided with DSC zooming port) Hex wrench (3 mm across flats) Hex wrench (4 mm across flats) First connect the quadrocular tilting tube and DSC zooming port for quadrocular tube, then attach them on the microscope arm (if epi-fluorescence cube turret or epi-fluorescence attachment is attached, on top of that). (1)

Remove the tightening screws at the bottom of the tube with the hex wrench (4 mm across flats).

(2)

Insert the positioning pin of the DSC zooming port while aligning it with the positioning hole on the tube side, and then tighten the connection fixing screws (2.5 mm across flats) on the top of the tube with a hex wrench.

(3)

Loosen the fixing screw on the front of the arm using a hex driver (2 mm across flats) so that the tip of the screw does not protrude into the connecting section. (The fixing screw at the front of the turret, if epi-fluorescence cube turret is attached.)

(4)

Loosen the fixing screws (x2) on both sides of the zooming port with a hex driver (2 mm across flats).

(5)

Align the round dovetail of the quadrocular tube with the round dovetail of the arm and slide the entire attached devices to the rear.

(6)

Tighten the fixing screw loosened in step (3).

(7)

Tighten the fixing screws (x2) on top of the zooming port with a hex wrench (3 mm across flats).

(8)

Tighten the fixing screws (x2) loosened in step (4).

(9)

Affix the provided sticker to cover the hole for the zooming port fixing screw.

Removing the tube tightening screw (Tube bottom view) Connection fixing screw Positioning hole

Connecting the quadrocular tube and DSC zooming port Zooming port fixing screw

1.

60

5

50

70

When attaching on the epi-fluorescence cube turret, the location of the fixing screws at the front is deep so it will be easier that you insert the hex driver (2 mm across flats) into the screw before placing the quadrocular tube or that you have a pen light when working.

Tightening screw

2

0.6

Set screw Fixing screw

Fixing the quadrocular tube and DSC zooming port set

8

Attach the EPI motorized shutter (optional). The motorized shutter is attached by Nikon. Contact your nearest Nikon representative when the EPI motorized shutter needs to be attached or removed.

10

Chapter 1

9

Assembly

Attach eyepieces. Notch

Protrusion

Assembly

60

50

70

Make sure the notch on the eyepiece side and the protrusion of the eyepiece sleeve are aligned, then insert the eyepieces. Notch on eyepiece The eyepiece has a notch to prevent rotation. When attaching, match the notch with the protrusion on the eyepiece sleeve. The eyepiece lens will not be positioned properly if the notch is not matched with the protrusion.

Attaching eyepieces

10 Attach the substage. Attach NI-SS substage or NI-SSR substage for rotatable stage suitable for the type of the stage used. The procedure is the same for both models. Tools: Hex driver (2 mm across flats) Hex wrench (3 mm across flats) (1)

(2)

Place the square groove on the substage onto the reference guide bar on the elevating section of the main body. Move the substage to the left, press it against the reference surface of the elevating section, and tighten the drop-proof fixing screws (x3) with hex wrench (3 mm across flats).

Substage fixing screw

Reference guide bar

Put the provided elevating section cover onto a front of the elevating section and tighten the provided two screws with hex driver (2 mm across flats) to secure. Square groove

Substage fixing screw (Substage from the front)

Attaching the substage

Elevating section cover

Attaching the elevating section cover

11

Chapter 1

Assembly

11 Attach the condenser holder. Attach a condenser holder to the substage. (Remove or attach the condenser holder after detaching the stage.) Hook the half dovetail of the condenser holder onto the half dovetail groove of the lower substage and push it against the reference surface. Then, tighten the fixing screw on the top of the substage.

Condenser holder fixing screw

Half dovetail

Half dovetail groove

Attaching the condenser holder

12 Attach the stage. 12.1 For standard stage (1)

Turn the coarse focus knob until the substage is brought to the lowermost position.

(2)

Loosen the stage clamp screw on the front of the stage.

(3)

Align the stage with the round dovetail of the substage and secure it in place with the stage clamp screw.

Stage clamp screw ND N 8 3 OUT

POWE

R IN

Securing the standard stage

12.2 For rotatable ceramic coated stage Tools: Hex driver (2 mm across flats) Centering tool: Ball-point hex drivers provided with the product (x2)

When removing the stage

80 70

90

60

Center the stage and then tighten the stage positioning screw. (See Chapter 3, “3 Bringing the Target into the Optical Path (Horizontal Stage Movement, Rotation) - ■Centering the rotatable ceramic coated stage” in the “Operation”)

50

(3)

40

Attach by engaging the round dovetail at the bottom of the stage with the round dovetail of the substage.

20

(2)

10

Sufficiently loosen the two right and left centering screws on the back of the substage and the stage positioning screw on the front of the stage using a hex driver.

0

(1)

30

Assembly

Tool: Hex wrench (3 mm across flats)

R POWE

Stage centering screws (on both sides)

Stage positioning screw

Securing the rotatable ceramic coated stage

When removing the stage, loosen the stage centering screws (x2) and stage positioning screw.

12

Chapter 1

Assembly

13 Attach the condenser. Tool: Hex driver (2 mm across flats)

(2)

Turn the condenser focus knob until the condenser holder reaches the bottom.

(3)

Fit the round dovetail of the condenser to the condenser holder so that the Nikon nameplate faces front and tighten the fixing screw of the condenser holder with a hex driver.

UD0 0 1 NI-C 6 9 6 JAPAN

Condenser fixing screw

Securing the condenser

■ Attaching the optical module to the universal condenser The turret has one empty hole and six optical module mounting holes. The empty hole is address 1 and the optical module cannot be attached here. The desired optical module can be mounted in the mounting holes of address 2 to 7. Tool: Hex driver (2 mm across flats) • DIC module on the condenser (required for differential interference contrast microscopy) There are DIC modules [D-C DIC N1 DRY], [D-C DIC N2 DRY], and [D-C DIC NR DRY]. Select the DIC module appropriate for the objective. (See following table.) If the combination is not correct, differential interference contrast image cannot be obtained or the contrast decreases significantly. To support for particular purposes, module for higher contrast or resolution are available. Note, however, that in principle the contrast contradicts the resolution of the differential interference contrast image (the higher the contrast, the lower the resolution). • PH module (required for phase contrast microscopy) There are PH modules [D-C PH-1], [D-C PH-2], and [D-C PH-3]. Select the PH module with a matching PH code with the PH objective. • Dark field module (required for dark-field microscopy) The dark field module [D-C DF] can be used with any objective with a numerical aperture (NA) of 0.7 or less. Note, however, that 2x and 4x objectives are not supported. • 2-4x auxiliary lens (required for low magnification bright-field microscopy) 2x and 4x objectives can only be used for bright-field microscopy. In this case, select a 2-4x auxiliary lens. Two types of 2-4x auxiliary lens are available with different diameter and different condenser turret attachment address: 2-4x auxiliary lens [NI-CAL N1] and 2-4x auxiliary lens [D-C 2-4x].

13

Assembly

Turn the coarse focus knob until the substage is brought to the upper limit.

0

(1)