BX51 and BX52 SYSTEM MICROSCOPE Instructions March 2000

BX51/52

CONTENTS Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to assemble the microscope yourself, please read section 7, “ASSEMBLY” (pages 27 to 29) carefully.

IMPORTANT - Be sure to read this section for safe use of the equipment. -

1-3

1

NOMENCLATURE

4-5

2

TRANSMITTED LIGHT BRIGHTFIELD OBSERVATION PROCEDURE

6-7

3

USING THE CONTROLS

8-20

3-1 Base ... 8-10 1 Voltage Indication; 2 Using the Light Intensity Preset Switch; 3 Using the Filters

3-2 Focusing Block ... 11 1 Replacing the Fine Adjustment Knob; 2 Adjusting the Coarse Adjustment Knob Tension; 3 Pre-focusing Lever

3-3 Stage ... 12-14 1 Placing the Specimen; 2 Adjusting the X- and Y-Axis Knob Tension; 3 Rotating the Stage;

4 Adjusting the Stage Height

3-4 Observation Tube ... 15-17 1 Adjusting the Interpupillar Distance; 2 Adjusting the Diopter; 3 Using the Eye Shades;

4 Using Eyepiece Micrometer Disks;

5 Selecting the Light Path;

6 Adjusting the Tilt

3-5 Condenser ... 18-19 1 Centering the Condenser; 2 Compatibility of Objectives and Condensers

3-6 Immersion Objectives ... 20 1 Using Immersion Objectives

3-7 Objectives with Correction Collar ... 20

4

TROUBLESHOOTING GUIDE

21-23

5

SPECIFICATIONS

24-25

6

OPTICAL CHARACTERISTICS

7

ASSEMBLY - See this section for the replacement of the light bulb. -

26

27-29

PROPER SELECTION OF THE POWER SUPPLY CORD ... 30-31

BX51/BX52

IMPORTANT This microscope employs a UIS (Universal Infinity System) optical design, and should be used only with UIS eyepieces, objectives and condensers for the BX2 series. (Some of the modules designed for the BX series are also usable. For details, please consult Olympus or the catalogues.) Less than optimum performance may result if inappropriate accessories are used.

SAFETY PRECAUTIONS

@

Fig. 1

(Fig. 1) 1. Install the microscope on a sturdy, level table or bench so as not to block the air vents on the underside of the base. Do not place the microscope on a flexible surface, as this could result in blocking the air vents and cause overheating or a fire. 2. The surfaces of the lamp housing on the rear of the microscope will become extremely hot during operation. When installing the microscope, make sure to allow ample free space (10 cm or more) around and in particular above the lamp housing. 3. When installing the microscope, route the power cord away from the lamp housing. Should the power cord come in contact with the hot lamp housing, the power cord could melt and cause electric shock. 4. To avoid potential shock hazards and burns when replacing the light ” (OFF) then disconnect the power bulb, set the main switch @ to “ cord from the wall outlet in advance. Whenever you replace the bulb during use or right after use, allow the lamp socket ‚ and bulb to cool before touching. (Fig. 1) Designated bulb

12V100WHAL (PHILIPS 7724) 12V50WHAL-L (LIFE JC)

# The microscope also incorporate a fuse (this should be replaced by the manufacturer or an authorized agent). 5. Always use the power cord provided by Olympus. If no power cord is provided, please select the proper power cord by referring to the section “PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of this instruction manual. If the proper power cord is not used, product safety performance cannot be warranted. 6. Always ensure that the grounding terminal of the microscope and that of the wall outlet are properly connected. If the equipment is not grounded, Olympus can no longer warrant the electrical safety performance of the equipment. 7. Never insert metallic objects into the air vents of the microscope frame as this could result in electrical shock, personal injury and equipment damage.

1

Safety Symbols The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment in the safest possible manner. Symbol

Explanation Indicates that the surface becomes hot, and should not be touched with bare hands. Before use, carefully read the instruction manual. Improper use could result in personal injury to the user and/or damage to the equipment.

l

Indicates that the main switch is ON.

Indicates that the main switch is OFF.

Warnings Warning engraving is placed at parts where special precaution is required when handling and using the microscope. Always heed the warnings. Warning engraving position

1

Getting Ready

Fig. 2

2

Lamp housing (Warning against high temperature)

1. A microscope is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact. 2. Do not use the microscope where it is subjected to direct sunlight, high temperature and humidity, dust or vibrations. (For the operating conditions, refer to section 5, “SPECIFICATIONS”.) 3. When moving the microscope, carefully carry it with the grasping part on the rear of the arm and the base as shown in Fig. 2 (Weight: approx. 16 kg). # If a module such as a photographic device is installed, the microscope system will be very heavy. Remove the intermediate attachment before carrying. # Damage to the microscope will occur if you grasp it by the stage, coarse/fine adjustment knob or binocular section of the observation tube. 4. The BX51/52 series can be used with up to two intermediate attachments (e.g. a U-CA magnification changer, U-EPA2 eyepoint adjuster, etc.). For restrictions when using two intermediate attachments, make sure to read the instruction manual provided with the respective intermediate attachments.

BX51/BX52

2

Maintenance and Storage 1. Clean all glass components by wiping gently with gauze. To remove fingerprints or oil smudges, wipe with gauze slightly moistened with a mixture of ether (70%) and alcohol (30%). Since solvents such as ether and alcohol are highly flammable, they must be handled carefully. Be sure to keep these chemicals away from open flames or potential sources of electrical sparks –– for example, electrical equipment that is being switched on or off. Also remember to always use these chemicals only in a well-ventilated room. 2. Do not attempt to use organic solvents to clean the microscope components other than the glass components. To clean them, use a lint-free, soft cloth slightly moistened with a diluted neutral detergent. 3. Do not disassemble any part of the microscope as this could result in malfunction or reduced performance. 4. When not using the microscope, keep it covered with a supplied dust cover.

3

Caution If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the equipment may also be damaged. Always use the equipment as outlined in this instruction manual. The following symbols are used to set off text in this instruction manual. : Indicates that failure to follow the instructions in the warning could result in bodily harm to the user and/or damage to equipment (including objects in the vicinity of the equipment). # : Indicates that failure to follow the instructions could result in damage to equipment. } : Indicates commentary (for ease of operation and maintenance).

3

1 NOMENCLATURE } If you have not yet assembled the microscope, read section 7, “ASSEMBLY” (pages 27 to 29). Transmitted light specification models

BX51TF or BX52TF microscope frame.

Light path selector knob (Page 16) Interpupillary distance adjustment scale (Page 15)

Condenser height adjustment knob (Page 18)

Allen screwdriver (accommodation position)

Diopter adjustment ring (Page 15)

Main switch (Page 1)

Voltage indicator LED Slide holder (Page 12)

Aperture iris diaphragm ring (Page 19)

Light intensity preset switch (Page 8)

Fine adjustment knob (Page 11) (Detachable) Pre-focusing Lever (Page 11)

Filter mount (Page 9)

Light intensity preset adjustment screw (Page 8)

Y-axis knob (Page 13)

X-axis knob (Page 13) Field iris diaphragm ring (Page 18) Coarse adjustment knob (Page 11) Brightness adjustment knob (Page 8) (Lamp voltage adjustment knob) Optional filter knob (Page 9) Coarse adjustment tension adjustment ring (Page 11) Filter knobs (Page 9) ND6, ND25 and LBD from the front to the rear

4

BX51/BX52

Transmitted light/reflected light specification models NOTE

BX51TRF and BX52TRF microscope frames

The controls are identical to those of the transmitted light specification models except for the transmitted/ reflected light switch and the reflected light system (vertical illuminator, reflected lamp housing).

Reflected light illuminator* · Universal illuminator (BX-URA2) · Fluorescence illuminator (BX-RFA)

Reflected lamp housing* · 100 W mercury burner housing (U-LH100HG) · 100 W mercury apo-burner housing (U-LH100HGAPO) · 75 W xenon lamp housing (U-LH75XEAPO) · 100 W halogen lamp housing (U-LH100-3) · System lamp housing (U-ULH, halogen bulb specification)

Transmitted/reflected ight switch (for exclusive use with the halogen light source)

* For the reflected illuminators and reflected lamp housings, please also refer to their instruction manuals.

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TRANSMITTED LIGHT BRIGHTFIELD OBSERVATION PROCEDURE

Set the main switch to “ I ” (ON) and adjust the brightness.

(Page)

@Main switch ²Brightness adjustment knob

(P. 8)

Set the transmitted light (BX51TRF/BX52TRF only)

³Transmitted/reflected light switch

(P. 5)

Engage only the LBD filter.

|LBD filter knob

(P. 9)

Select the light path (trinocular tube).

ƒLight path selector knob

(P. 16)

Place the specimen on the stage.

…Slide holder †X-/Y-axis knobs

(P. 12) (P. 13)

Engage the 10X objective in the light path.

‡Revolving nosepiece

ŠCoarse/fine adjustment knobs

(P. 11)

‰Binocular tube ‹Diopter adjustment ring ŒCondenser height adjustment knob ™Condenser centering knob

(P. 15) (P. 15) (P. 18)

Adjust the aperture iris and field iris diaphragms.

šAperture iris diaphragm ring ›Field iris diaphragm ring

(P. 19) (P. 18)

Engage the objective to be used in the light path and bring the specimen in focus.

‡Revolving nosepiece

Bring the specimen in focus.

Adjust the interpupillary distance. Adjust the diopter. Adjust the light axis.

(P. 18)

Engage the required filters.

œFilters

(P. 9)

Adjust the brightness.

²Brightness adjustment knob

(P. 8)

Start observation.

6

(Controls Used)

BX51/BX52

‰ ƒ

Œ

@ ‹ ³

‡ …

š ™

†

Š

›

²

Š

œ

|

} Copy the observation procedure pages on separate sheets and post it near your microscope.

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USING THE CONTROLS 3-1 Base 1

Voltage Indication

(Fig. 3)

1. Turn the brightness adjustment knob @ clockwise to increase the voltage and make illumination brighter. 2. The numerals to the right of the lamp voltage indicator LEDs ² indicate the voltage.

@

²

Fig. 3

2

Using the Light Intensity Preset Switch

(Fig. 4)

}The light intensity preset switch @ makes it possible to limit the light intensity to a preselected level regardless of the position of the brightness adjustment knob. The light intensity preset has been preset to optimum brightness for photography when the built-in LBD filter is engaged (approx. 9 V with marking). 1. Press the light intensity preset switch @ to the ON position. (The face of the switch lights when it is ON.) 2. Using a small flat-blade screwdriver, turn the preset adjustment screw ² to obtain the required light intensity. Turning the screw clockwise increases brightness. 3. When the light intensity preset switch is set to OFF, the brightness returns to the level set by the brightness adjustment knob. # While the light intensity preset switch is ON, turning the light brightness adjustment knob does not affect brightness.

@ ² Fig. 4

3

Using the Filters

(Figs. 5 - 10)

}You can place a filter in the light path with one of the following methods. · Push in the knob of the desired built-in filter to engage it in the light path. (Page 9) · Place a filter on the filter mount on the base and engage in the light path. (Page 9) · Insert a filter in the U-FC filter cassette, attach it on the filter mount and engage it in the light path by sliding the filter lever. (Page 9)

8

BX51/BX52

Using Built-In Filters (Fig. 5) Each of filter knobs @ to | can be engaged into the light path by pressing the knob in. Pressing the button again disengages the filter from the light path. Filter Type/Purpose

@ ²³ | Fig. 5

@

ND6 (Neutral Density filter for light adjustment, transmittance 6%)

²

ND25 (Neutral Density filter for light adjustment, transmittance 25%)

³

LBD (for color balancing, daylight filter)

|

OP (optional) filter set*

* The set can be ordered at your local Olympus representative. Mounting a Single Filter

ƒ

(Fig. 6)

A filter with a diameter of 45 mm ƒ can be placed on the filter mount on the base. If you need to place multiple filters, please purchase the U-FC filter cassette. # Even when a filter cassette is used, a filter with thickness of no more than 3 mm can still be placed on the filter mount.

Fig. 6

Using the Filter Cassette

(Figs. 7 - 10)

Loading Filters into Filter Cassette

…

Fig. 7

}The filter cassette accommodates filters with a diameter of 45 mm and thickness of 2.7 mm or less. }The filter cassette has two filter levels on the right side and one on the left side. 1. Move all filter levers to the OUT position except for the one belonging to the slot into which the filter is to be inserted. 2. Slide the lever … to the IN position. Make sure that it clicks securely into place. (Fig. 7) 3. Holding the lever in the position shown in Fig. 7, put the filter into the cassette by inserting it in the direction indicated by the arrow. 4. Insert the other two filters in the same manner.

9

Mounting the Filter Cassette

‡ † Š ‰

Fig. 8

1. Fully loosen the filter cassette clamping screw †. (Fig. 8) 2. Align the key ‡ on the bottom surface of the filter cassette with the positioning slot Š on the filter mount, then snap the filter cassette into place from above. 3. Rotate the filter cassette to align its sides with the base. (Fig. 9) 4. Align the clamping screw † with the positioning hole ‰ on the filter mount, then tighten the screw to fasten the filter cassette. # When the filter cassette is installed, the stage may hit it when lowered. Therefore, exercise caution when lowering the stage with the filter cassette installed.

Fig. 9 Using the Filter Cassette

(Fig. 10)

Usable Filters

‹ Fig. 10

10

Applications

45ND-6, 45ND-25

Neutral density filter

45G-530, 45G-533, 45IF550

Green

45Y-48

Yellow

45O-560

Orange

B&W contrast filter

Up to three of the above filters can be loaded in the filter cassette. Moving the levers ‹ on the left and right sides of the cassette to the IN position brings the corresponding filter into the light path.

BX51/BX52

3-2 Focusing Block 1

³

²

@

2

3

Fig. 13

Adjusting the Coarse Adjustment Knob Tension

(Fig. 12)

# Adjust the coarse adjustment knob tension using the tension adjustment ring @. The coarse adjustment knob tension is preadjusted for easy use. However, if desired, you can change the tension using the tension adjustment ring @. Turning the ring in the direction of the arrow increases tension, and vice versa. The tension is too low if the stage drops by itself of focus is quickly lost after adjustment with the fine adjustment knob. In this case, turn the ring in the direction of the arrow to increase tension.

Fig. 12

@

(Fig. 11)

}The fine adjustment knob is designed detachable to prevent interference with hand during manipulation of the fine adjustment knob or X-/Y-axis knobs. Usually attach the knob on the opposite side to the X- and Y-axis knobs. 1. Using the Allen screwdriver, loosen the clamping screw @ and remove the fine adjustment knob ². 2. Remove the seal from the fine adjustment knob screw hole on the other side and attach the knob by reversing the removal procedure. 3. Attach a provided seal on the screw hole of the removed fine adjustment knob ³. }The fine adjustment knob can be operated with your fingertip or finger surface at the same time as manipulating the X- or Y-axis knob.

Fig. 11

@

Replacing the Fine Adjustment Knob

Pre-focusing Lever

(Fig. 13)

}The pre-focusing lever ensures that the objective does not come in contact with the specimen and simplifies focusing. After focusing on the specimen with the coarse adjustment knob, turn this lever @ in the direction of the arrow and lock; the upper limit on coarse adjustment movement is set at the locked position. After changing a specimen, refocusing is easily accomplished by rotating the coarse adjustment knob to reach the pre-focused position, then making fine adjustments with the fine adjustment knob. }Stage movement with the fine adjustment knob is not locked. # If you want to lower the stage to the lower limit, release the lock in advance.

11

3-3 Stage ²

@

1

Placing the Specimen # The dimensions of the slide glass should be 26 x 76 mm with thickness of 0.9 to 1.2 mm, and the cover glass should have thickness of 0.17 mm. # When observing very large specimens, remove the slide holder and place the specimen directly on the stage. Microscopy with Double-Slide Holder (Fig. 14) 1. Turn the coarse adjustment knob @ to lower the stage. 2. Open the spring-loaded curved finger ² on the slide holder and place one or two specimen slides on the stage from the front. 3. After placing the sides as far as they will go, gently release the curved finger.

Fig. 14

Microscopy with Single-Slide Holder (Fig. 15) The specimen side can easily be placed by sliding it into the slide holder from the front.

Fig. 15 Using an Oil Immersion Objective Adsorption of immersion oil can cause the specimen to drift. In such cases, it is recommended to use the optional BH2-SCB-3 specimen clip ³ for oil immersion objectives. (Fig. 16) Using an Oil Immersion Condenser

³ Fig. 16

12

It may be effective to use the optional U-SVRO (right-hand knob) or USVLO (left-hand knob) slotted stage which prevents close contact between the specimen and stage.

BX51/BX52

2

Adjusting the X- and Y-Axis Knob Tension

(Fig. 17)

1. Hold the X-axis knob @ and slide up the Y-axis knob ² up to expose the adjustment knobs. 2. Turning the X-axis adjustment knob ³ or Y-axis adjustment knob | clockwise (in the direction of the arrow) increases the tension and counterclockwise decreases it. # If the tension is adjusted to tight, a creaking sound may be heard during stage travel, and the stage stopping accuracy may be imperiled.

² | ³ @ Fig. 17

CAUTION

After long hours of use, the stage guide may be deviated and the stage travel range may be decreased. However, this is not malfunction and can be corrected easily as described below.

[Treatment] Horizontal direction: Hold the specimen holder and move the stage guide to the left and right so that it hits the stoppers. Vertical direction: Hold the upper stage and move it to the front and rear so that it hits the stoppers. Stage Feed Knob Rubber Caps (Optional) }When the X- and Y-axis knobs are fitted with the rubber caps, the knobs can be adjusted without slipping and fine adjustment is possible by holding the knobs with a very light force. The knob rubber caps also reduce fatigue after long hours of operation. The U-SHGT thick type (thickness 5 mm) and U-SHG thin type (thickness 2 mm) knob rubbers are available. To attach the knob rubbers: First fit the larger knob rubber to the Y-axis (upper) knob from below it, then fit the smaller knob rubber to the X-axis (lower) knob from below it.

13

3

Rotating the Stage

(Fig. 18)

1. Slightly loosen the stage clamping screw @. 2. The stage can be rotated both clockwise and counterclockwise by the stage clamping screw. # A click may be heard and felt during rotation. However, this is due to the construction of the substage and does not indicate a malfunction. }The angle of rotation varies depending on the positions of the X- and Yaxis knobs.

@ Fig. 18

Angle of Rotation

4

@ Fig. 19

²

³

Fig. 20

14

Clockwise

Counterclockwise

Right hand knobs

230°

20°

Left hand knobs

20°

230°

Adjusting the Stage Height

(Figs. 19 & 20)

}By lowering the position of the substage, the microscope will accommodate specimens with maximum height of 35 mm. This is useful when observing metallurgical specimens and other thick objects. 1 Lower the stage to the lower limit, then remove the stage from the microscope. 2 Using the Allen screwdriver, loosen the substage bracket clamping screw @ and remove the substage. 3. Turn the coarse adjustment knob and raise the focusing block ³ to where the stopper screw ² on the arm becomes visible. 4. Using the Allen screwdriver, loosen and remove the upper stopper screw ². 5. Reattach substage bracket and stage.

BX51/BX52

3-4 Observation Tube 1

Adjusting the Interpupillar Distance

(Fig. 21)

While looking through the eyepieces, adjust for binocular vision until the left and right fields of view coincide completely. The index dot · indicates the interpupillary distance. }Note your interpupillary distance so that it can be quickly duplicated.

Fig. 21

@

2

Adjusting the Diopter

(Figs 22 & 23)

1. Looking through the eyepiece without the diopter adjustment ring, rotate the coarse and fine adjustment knobs to bring the specimen into focus. 2. Looking through the eyepiece with the diopter adjustment ring, turn only the diopter adjustment ring @ to focus on the specimen. (Fig. 22)

² Fig. 22 Using a Finder Eyepiece 1. Looking through the right eyepiece with your right eye, turn the top of the eyepiece ² until a clearly defined double crossline can be seen in the field of view. (Fig. 22 & 23) 2. Looking through the right eyepiece, rotate the coarse and fine adjustment knobs to bring the specimen and double crossline into simultaneous focus. 3. Looking through the left eyepiece with your left eye, turn the diopter adjustment ring @ to focus on the specimen. Fig. 23

3

Using the Eye Shades

(Fig. 24)

When Wearing Eyeglasses Use with the eye shades in the normal, folded-down position. This will prevent the eyeglasses from being scratched. When Not Wearing Eyeglasses Extend the folded eye shades in the direction of the arrow to prevent extraneous light from entering between the eyepieces and eyeglasses. Fig. 24

15

4 @

Using Eyepiece Micrometer Disks

(Fig. 25)

Eyepiece micrometer disks can be inserted into WH10X-H (or WH10X) eyepieces. Use 24 mm diam. x 1.5 mm thick micrometer disks. Following Fig. 25, unscrew the micrometer mounting frame ² from the eyepiece and place a micrometer disk @ into the mounting frame. The engraving on the micrometer disk should face downward into the micrometer mounting frame. Screw the micrometer mounting frame back into the eyepiece sleeve.

²

Fig. 25

@

5

Selecting the Light Path (with the U-TR30-2 or U-SWTR-2)

(Fig. 26)

Slide the light path selector knob @ to select the desired light path. Light Path Selector Knob

Intensity Ratio

Applications

100% for binocular eyepieces

Observation of dark specimens

Middle position

20% for binocular eyepieces, 80% for TV/photography

Observation of bright specimens, photography, TV observation

Pulled out

100% for TV/ photography

Photography, TV observation

Pushed in

Fig. 26

16

Symbol

BX51/BX52

6

Fig. 27

Adjusting the Tilt (with the U-TBI3)

(Fig. 27)

}Adjust the height and tilt of the observation tube to obtain the most comfortable viewing position. Holding the binocular section with both hands, raise or lower it to the desired position. # Never attempt to force the binocular section past the upper or lower stop position. Applying excessive force could destroy the limiting mechanism. }The U-TBI3 can be used in combination with only one intermediate attachment. }If you need photography using the U-TBI3, use the U-TRU trinocular tube. With the U-ETBI (Fig. 28) The U-ETBI is an ergonomic observation tube with normal field capable of tilting adjustment with an erect image and the eyepiece position can be adjusted by 45 mm toward the front and rear.

Fig. 28

17

3-5 Condenser 1

³

@

² Fig. 29

Centering the Condenser

(Figs. 29 & 30)

1. Turn the condenser height adjustment knob @ to raise the condenser to its upper limit. 2. Focus on the specimen using the 10X objective. # When using the U-SC3 swing-out condenser, move the top lens into the light path. 3. Rotate the field iris diaphragm ring ² in the direction of the arrow so that the diaphragm image comes inside the field of view. 4. Manipulate the condenser height adjustment knob @ to focus on the diaphragm image 5. Turn the two condenser centering screws ³ to move the iris diaphragm image to the center of the field of view. 6. Gradually open the field iris diaphragm. The condenser is properly centered if the iris image is centered and inscribed in the field of view. 7. During actual use, open the field diaphragm slightly until its image circumscribes the field of view.

Effects of Field Iris Diaphragm (Fig. 30) The field iris diaphragm restricts the diameter of the beam of light entering the objective and thus excludes extraneous light, improving image contrast. The diameter of the field iris should e adjusted for objective power to the extent that it just circumscribes the field of view. (See “Compatibility of Objectives and Condensers” on the next page.) Fig. 30

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