Thermo Scientific
Cystospin 4 Operators Manual Issue 6 2015
Operators Manual
75 Pages
Preview
Page 1
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Accessories ... 23 Appendices ... 60 Appendix A – Standard Workflow Diagram ... 60 Appendix B – Methodology Guidelines ... 62 Appendix D – Approved Reagent List... 79 Approved Reagent List Cleaning Agents ... 79 Reagent List... 79 Approved Reagents... 14
General Safety ... 5 General Theory – General laboratory Considerations .. 62 General Theory – Introduction... 62
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Cells / 40x field as a guideline to the number of drops of cell suspension / Thermo Scientific Cytospin 4 sample chamber ... 65 Chemical Safety ... 7 Cleaning and Maintenance ... 44 Routine Cleaning and Maintenance ... 45 Company Information ... 3 Cone Seal ... 48 Control Keypad ... 31 Controls ... 28 Main Control Panel ... 28
Installation and Setup ... 16 Instrument Function Error Codes ... 50 Introduction ... 74 Introduction to the Cytospin 4 Description ... 13 Introduction to the Thermo Scientific Cytospin 4 System Specifications ... 15 Introduction to Thermo Scientific Cytospin 4 ... 13 IVD Intended Use ... 13
H Haematology and other clinical Microscopy Specimens ... 74
L E Electrical Requirements ... 21 Electrical Specification ... 15 EMC Statement ... 4 Entering the Acceleration Required ... 42 Entering the Speed Required ... 41 Entering the Time Required ... 41 Environment ... 7 Environmental Specifications ... 15 Error Codes, Audible Tones and Warnings ... 43 EZ Double Cytofunnel ... 25 EZ Megafunnel ... 26 EZ Megafunnel End of Spin Processing Run ... 27 EZ Single Cytofunnel ... 23
F Front Panel... 45
Lid Seal ... 49 Lid, Case, Plinth and Bowl Liner ... 45 Loading the EZ Cytofunnel ... 37 Loading the Sealed Head... 38
M Mechanical Specification ... 15 Method of Operation ... 33 Methodology Guidelines ... 75
O Opening and Closing the Cytospin 4 Lid ... 18 Opening and Closing the Sealed Head ... 19 Operation Loading the Cytospin 4 ... 37 Operating the Thermo Scientific Cytospin 4 ... 35 Optimal Thermo Scientific Cytospin 4 Technique ... 76
P Program Details ... 15 Program Keypad ... 28
S Safety Information ... 5 Saving a Program ... 40 Seal Replacement ... 48 Sealed Head Assembly ... 46 Sealed Head Base ... 46 Sealed Head Lid... 47 Selecting a Program ... 40 Siwtch Convention ... 15 Sound Power level ... 15 Spares and Accessories ... 58 Special Considerations ... 71 Cell Adhesion ... 71 Cytology ... 71 Haematology ... 72 Microbiology ... 72 Urine Specimens ... 72 Specimen Preparation ... 63 Determination of Cell Number ... 64 Evaluation of Specimen to Assess Technique ... 69 Fixation ... 69 Initial Examination ... 63 Loading the Thermo Scientific Cytospin 4 Sample Chambers ... 67 Selecting Time, Speed and Acceleration ... 68 Specimen Dilution ... 67 Specimen Enrichment ... 66 Unloading the Sample Chambers ... 68
Starting a Run... 42 Status Display ... 32 Support Plate ... 47 Switching On and Off ... 22 Symbols ... 6
T Table 2 – Error Codes ... 51 Table 3 - Unsatisfactory Cytocentrifugation Results – Quality ... 52 Table 4 - Unsatisfactory Cytocentrifugation Results – Quality (Number of Cells) ... 54 Table 5 – Unusual Pattern of Cell Population Distribution ... 55 Table 6 – Summary ... 56 To Switch Off ... 22 To Switch On ... 22 Transportation Instructions ... 77 Repacking ... 77 Troubleshooting ... 50 Instrument Function ... 50
U Unloading the Thermo Scientific Cytospin 4 ... 42 Unpacking ... 17 Uses and Application... 74
W Warranty Statement ... 7