Thermo Scientific

NanoDrop 2000, 2000c and 1000 Calibration Check Rev Feb 2009

Guide

2 Pages

INSTRUCTIONS  NanoDrop 2000/2000c & 1000  Calibration Check A vial of CF-1 (aqueous potassium dichromate (K2Cr2O7) solution) is required to run the calibration check procedure for the Thermo Scientific NanoDrop 2000/2000c and NanoDrop 1000 Spectrophotometers. Procedure 1. Ensure the measurement pedestals are clean and that a 1ul water sample “beads” up on the lower pedestal. 2. Remove any lint build-up from around the instrument solenoid by following the instructions on the back page of this document. 3. Open the Calibration Check Software and follow the prompts in the Customer Guidance text box of the software. 4. Enter the Target Absorbance found on the CF-1 vial as directed in the images below. 5. Add 1ul of deionized water and select “Blank”. 6. Before opening the ampoule of CF-1 Calibration Fluid, shake vigorously to ensure solution is thoroughly mixed. Ensure all solution is collected in the bottom portion of the ampoule. 7. Carefully break the neck of the ampoule to open the CF-1 Calibration Fluid. 8. Follow the on-screen prompts in the Customer Guidance text box. Using individual 1ul samples of the CF-1 Calibration Check Fluid, measure 10 replicates. 9. After the 10th measurement, the calibration check results will be displayed on-screen in the Customer Guidance text box. If the instrument does not pass the calibration check using 1ul samples, immediately rerun the procedure (step 8) using 2ul samples. 10. The NanoDrop™ 2000/2000c software will archive the results in the autosave file at C:Documents and SettingsAll UsersDocumentsThermoNanoDrop2000AutoSaveCalibCheck. 11. The NanoDrop 1000 software will archive the results will in the NanoDrop data folder at: C:NanoDrop DataCalib check.. 12. If using the NanoDrop 1000 calibration check specific software downloaded from our website, the results are not automatically archived and must be manually saved to retain a record. 13. If recalibration is required, contact us @ 302-479-7707 or by email at nanodrop@thermofisher.com. Note The CF-1 Calibration Fluid is supplied in a single use vial. The CF-1 must be used within one hour of opening the vial. Exposure to the environment or transferring of the fluid to another container may cause a significant concentration change.  NanoDrop 1000 Calibration Check Screen  Thermo Fisher Scientific - NanoDrop Products  Wilmington, Delaware USA  NanoDrop 2000/2000c Calibration Check Screen  Technical support: info@nanodrop.com  302-479-7707  www.nanodrop.com
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