Thermo Scientific

NanoDrop 2000 and 2000c User Manual Ver 1.0 Revised March 2009

User Manual

97 Pages

Table of Contents 1. INTRODUCTION ... 1-1 INSTRUMENT DESCRIPTION ... 1-1 INSTRUMENT SPECIFICATIONS ... 1-1 Patents... 1-1 SAMPLE RETENTION PEDESTAL MEASUREMENTS ... 1-2 Pedestal Basic Use ... 1-2 CUVETTE MEASUREMENTS... 1-3 Cuvette Basic Use ... 1-3 BLANKING AND ABSORBANCE CALCULATIONS ... 1-4 FLUORESCENT DYES ... 1-5 2. SOFTWARE... 2-1 COMPUTER REQUIREMENTS ... 2-1 SOFTWARE INSTALLATION ... 2-1 Registering Your Instrument... 2-2 SOFTWARE FEATURES ... 2-2 Left Pane ... 2-2 Right Pane... 2-5 DATA AND ACCOUNT MANAGEMENT ... 2-7 My Data ... 2-7 Reports ... 2-7 Autosave files... 2-9 Options... 2-9 3. APPLICATIONS... 3-1 OVERVIEW ... 3-1 QUICK START ... 3-1 MEASUREMENT RANGES ... 3-2 NUCLEIC ACID ... 3-3 Overview... 3-3 Nucleic Acid Calculations ... 3-3 Unique Screen Features ... 3-4 Making Nucleic Acid Measurements... 3-5 Oligo Calc ... 3-6 MICRO ARRAY ... 3-8 Overview... 3-8 Dye/Chromophore Editor... 3-8 Unique Screen Features ... 3-8 Making Micro Array Measurements ... 3-9 Oligo Calc ... 3-10 UV-VIS ... 3-13 Overview... 3-13 Unique Screen Features ... 3-13 Making UV-Vis Measurements ... 3-14 PROTEIN A280 ... 3-16 Overview... 3-16 Unique Screen Features ... 3-17 Making Protein A280 Measurements... 3-18 PROTEINS & LABELS ... 3-20 Overview... 3-20 Dye/Chromophore Editor... 3-20 Unique Screen Features ... 3-21 Making Proteins & Labels Measurements ... 3-22 PROTEIN BCA ... 3-24 Overview... 3-24 Unique Screen Features ... 3-25 Making BCA Assay Measurements ... 3-27 PROTEIN LOWRY ... 3-29 Overview... 3-29
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