Thermo Scientific

Small Benchtop Effective and Efficient Application Note Oct 2014

Application Note

4 Pages

Richard Sicard, Thermo Fisher Scientific, Asheville, North Carolina, USA Romana Hakim, Thermo Fisher Scientific, Osterode am Harz, Germany  Appli c a ti on N ote  Using Thermo Scientific Small Benchtop Centrifuges for the Effective and Efficient Processing of Clinical Samples  Key Words Small benchtop centrifuge, Platelet-poor plasma (PPP), Urinalysis, Blood chemistry analysis  Introduction Clinical laboratory testing plays an important role in the detection, diagnosis, and treatment of disease. The use of centrifugal force for separation of blood and urine is a very crucial step within the process. It is very important not only to perform this step correctly, but also efficiently so as to derive the best results for the patient sample. Blood is typically separated to yield platelet-poor plasma (PPP), which in turn is used for most coagulation tests. Ideally, PPP should have a platelet count of less than 10 x 103 per µL1. Similarly, the analysis of urine is also very important in the clinical laboratory. Information derived from this testing can predict many important medical concerns, one of which is the function of kidneys or associated infections. This common test measures various compounds such as white blood cells, red blood cells, epithelial cells, bacteria, calcium oxalate crystals, mucous threads, yeast, and hyaline casts that pass through the urine2, 3.  capacity than the standard swinging bucket rotor, this rotor’s higher speed does make it a very important rotor in efficiently and effectively processing PPP. These small benchtop centrifuges offer sample protection with the Thermo Scientific™ ClickSeal™ biocontainment lid system certified by Public Health England, Porton Down, UK. They also provide Thermo Scientific™ Auto-Lock™ rotor exchange which allows rotors to be installed and removed from the chamber of the centrifuge with the push of a button – no tools required. Table 1. Thermo Scientific™ Small Benchtop Centrifuges Heraeus™ Megafuge™ 8 small benchtop centrifuge, ventilated Heraeus™ Megafuge™ 8R small benchtop centrifuge, refrigerated Sorvall™ ST 8 small benchtop centrifuge, ventilated Sorvall™ ST 8R small benchtop centrifuge, refrigerated SL 8 small benchtop centrifuge, ventilated SL 8R small benchtop centrifuge, refrigerated  Finally, blood chemistry analysis is also a very common test in the clinical laboratory. These results are used to evaluate a variety of components such as potassium, aspartate aminotransferase (AST), phosphorus, and lactate dehydrogenase (LDH)4. The objective of this note is to illustrate the efficiency and effectiveness of Thermo Scientific™ small benchtop centrifuges in critical centrifugation steps of the following clinical testing: (A) platelet-poor plasma (PPP) preparation, (B) urinalysis, and (C) blood chemistry analysis. Thermo Scientific small benchtop centrifuges (see Table 1) can be used with the Thermo Scientific TX-150 swinging bucket rotor for high-speed horizontal processing. The TX-150 rotor accommodates up to 24 x 5/7 mL blood collection tubes or 8 x 50 mL conical tubes at 4500 rpm (3260 x g) but when configured with the Thermo Scientific™ HIGHConic™ rotor, it can achieve up to 8,700 rpm (10,155 x g) with 6 tubes. While a lower  Figure 1. Thermo Scientific Small Benchtop Centrifuge.
File Type: PDF
File Size: 547 KB
File Name: small_benchtop_effective_and_efficient_application_.pdf

By clicking Download you are confirming this is the correct document for your purposes
and that you agree to our Terms & Conditions