Superspeed FA Rotor Models SE-12, SS-34, SM-24, SA-300, SA-600 Operation User -Maintenance Manual

Preface

Important Safety Information Certain potentially dangerous conditions are inherent to the use of all centrifuge rotors. To ensure safe operation of this rotor, anyone using it should be aware of all safe practices and take all precautions described below and throughout this manual. WARNING When using radioactive, toxic, or pathogenic materials, be aware of all characteristics of the materials and the hazards associated with them in the event leakage occurs during centrifugation. If leakage does occur, neither the centrifuge nor the rotor can protect you from the particles dispersed in the air. To protect yourself, we recommend additional precautions be taken to prevent exposure to these material, for example, use of controlled ventilation or isolation areas. Always be aware of the possibility of contamination when using radioactive, toxic, or pathogenic materials. Take all necessary precautions and use appropriate decontamination procedures if exposure occurs. Never use any material capable of producing flammable or explosive vapors or creating extreme exothermic reactions. Never exceed the maximum rated speed of the installed rotor; to do so can cause rotor failure. Always reduce (derate) rotor speed as instructed in this manual whenever: •

the rotor speed/temperature combination exceeds the solubility of the gradient material and causes it to precipitate.

•

the compartment load exceeds the maximum allowable compartment load specified. See Chapter 2, paragraph 2-2.

Failure to reduce rotor speed under these conditions can cause rotor failure.

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Superspeed Fixed-Angle Rotors

Thermo Fisher Scientific

Preface

CAUTION Do not expose aluminum rotor components to: strong acids, bases, or alkaline laboratory detergents; liquid chlorine bleach; or salts (chlorides) of heavy metals such as cesium, lead, silver, or mercury. Use of these materials with aluminum can cause a chemical reaction that initiates corrosion. Do not operate or precool the rotor at the critical speed, as this will have a detrimental effect on centrifuge component life. See Chapter 2, paragraphs 2-3. Do not operate the rotor unless it is symmetrically balanced as described in this manual. Operating the rotor out of balance can cause damage to the centrifuge drive assembly. Do not operate the rotor without the cover in position and locked in place and the rotor locked to the centrifuge drive. See Chapter 2, paragraph 2-6. Always maintain the rotor in the recommended manner. The rotor and all accessories must be clean and inspected prior to each run: do not use rotors showing signs of corrosion or cracking. See Chapter 3, Care and Maintenance. Do not autoclave or expose any aluminum rotor parts to temperatures in excess of 121°C.

Thermo Fisher Scientific

Superspeed Fixed-Angle Rotors

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Preface

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Superspeed Fixed-Angle Rotors

Thermo Fisher Scientific

Contents Preface

Important Safety Information ... ii

Chapter 1 Description Rotor Description... 1-1 Rotor Specification ... 1-2 Replacement Parts ... 1-3 Tubes, Bottles and Adapter ... 1-6 Chapter 2 Operation Prerun Safety Checks... 2-1 Compartment Loads in Exess of Design Mass... 2-1 Critical Speed ... 2-2 Relative Centrifugal Force (RCF) Determination ... 2-3 Calculation of Sedimentation Time in Aqueous (Non-Gradient) Solutions ... 2-4 Rotor Loading, Balancing and Sealing ... 2-5 Rotor Installation ... 2-8 Rotor Speed/Temperature Differential Determination . 2-9 Capter 3

Care and maintenance Corrosion ... 3-1 Cleaning ... 3-2 Storage ... 3-2 Inspection ... 3-3 Rotor Seal Integrity ... 3-3 Service Decontamination Policy ... 3-6

Appendix RCF Values and K Factor Tables... 4-1 Warranty ... 4-16 Chemical Compatibility Chart ... 4-17 Blank Rotor Speed/Temperature Differential Graphs. 4-24

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Superspeed Fixed-Angle Rotors

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Contents

List of Illustrations Parts Location: SA-600, SE-12, SM-24 and SS-34 Rotors... 1-4 Parts of the SA-300 Rotor ... 1-5 Cross Section of Superspeed Fixed-Angle Rotor Showing Radial Distances ... 2-3 Determining the Minimum Radius of a Partially-Filled Tube or Bottle... 2-4 Rotor Balancing... 2-6 Properly Seated ULTRACRIMP® Rotor Cap... 2-8 Sample Rotor Speed/Temperature Differential Graphs (Continued) ... 2-11

List of Tables Rotor Specifications ... 1-1 Parts Identification for the SA-600, SE-12, SM-24 and SS-34 Rotors... 1-3 Parts Identification for the SA-300 Rotor... 1-5 Maximum Compartment Mass for Each Rotor ... 2-2 Critical Speed... 2-2 Rotor Balancing... 2-6 SE-12 Rotor: Sealed Tube or Bottle with Maximum Allowable Volume... 4-1 SE-12 Rotor: Unsealed Tube or Bottle with Maximum Allowable Volume... 4-2 SM-24 Rotor: Sealed Tube or Bottle with Maximum Allowable Volume - OUTER ROW ... 4-4 SM-24 Rotor: Sealed Tube or Bottle with Maximum Allowable Volume - INNER ROW ... 4-6 SM-24 Rotor: Unsealed Tube or Bottle with Maximum Allowable Volume - OUTER ROW ... 4-7 SM-24 Rotor: Unsealed Tube or Bottle with Maximum Allowable Volume - INNER ROW ... 4-8 SS-34: Rotor: Sealed Tube or Bottle with Maximum Allowable Volume... 4-10 SS-34: Rotor: Unsealed Tube or Bottle with Maximum Allowable Volume... 4-11 SA-300: Rotor: Sealed Tube or Bottle with Maximum Allowable Volume... 4-13 SA-300: Rotor: Unsealed Tube or Bottle with Maximum Allowable Volume... 4-14 Chemical Compatability Charts... 4-17

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Superspeed Fixed-Angle Rotors

Thermo Fisher Scientific

Chapter 1 DESCRIPTION This manual contains information required to operate and maintain the Thermo Scientific Superspeed Fixed-Angle Rotors: SA-600, SA-300, SE-12, SM-24 and SS-34. If you require additional information regarding operation or maintenance, please contact Thermo Fisher Scientific for assistance. In the United States, call Thermo Fisher Scientific toll-free 1-866-9THERMO; outside the United States, contact the nearest Thermo Fisher Scientific office (see back cover) or your local representative for Thermo Fisher Scientific products. Thermo Scientific product information is available on our internet web site at http://www.thermo.com/centrifuge .

Rotor Description

Thermo Scientific Superspeed Fixed-Angle Rotors are designed for use in the Thermo Scientific RC-28S SUPRAspeed® Centrifuge1 RC-26 PLUS, RC-5C PLUS, RC-5C, RC-5B PLUS, RC-5B, RC-6, RC-6+, and Evolution RC Superspeed Centrifuges. The rotors are machined from high strength-to-weight ratio aluminum alloy forgings. For added safety, the SA-300, SA-600, SE-12, SM-24, and SS-34 rotors are designed to provide biocontainment up to ACDP Category 3.2

Rotor Specifications

Table 1-1 provides the basic specifications for each of the rotors described in this manual.

Table 1-1. Rotor Specifications Diameter Mass (weight)

SE-12

SS-34

SM-24

SA-300

SA-600

19 cm (7.8 in)

23 cm ( 9 in)

24 cm (9.13 in)

21 cm (8.27 in)

27 cm (10.75 in)

4.1 kg

6.8 kg (15 lbs)

7.7 kg (17 lbs)

5.6 kg (12 lbs)

10.1 kg (22.5 lbs)

(9 lbs)

1The SA-300 Rotor can not be used in the RC-28S Centrifuge.

2Advisory Committee on Dangerous Pathogens (1984), Categorization of pathogens according to hazard and categories of containment, HMSO, ISBN 0 11 883781 3. Sealed rotors are intended to be part of a biosafety system such as are specified in international and national biosafety guidelines. Do not rely upon them as the only means of safeguarding yourself and the environment when handling pathogenic microorganisms.

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Superspeed Fixed-Angle Rotors

1-1

DESCRIPTION Rotor Specifications

Table 1-1. Rotor Specifications SE-12

SS-34

SM-24

SA-300

SA-600

Tube Angle Number of Places

40°

34°

28°

34°

34°

12

8

24

6

12

Maximum Rotor Speed* (in rpm)

26,000

20,500**

20,500**

25,000

17,000**

Relative Centrifugal Force (RCF) at Maximum Speed***

70,450

50,228

51,965 (Outer Row)

67,509

41,837

K Factor at Maximum Speed**

335

115g

115g

42,717 (Inner Row) 714

434 (Outer Row) 591 (Inner Row)

Maximum Compartment Mass

30g

115g

27g

* Speed in revolutions per minute (rpm) is related to angular velocity, w, according to the following:

Where ω = rad/s. All further references in this manual to speed will be designated as rpm. **Maximum rotor speed is dependent on the centrifuge used. ***

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Superspeed Fixed-Angle Rotors

With a sealed tube or bottle containing the maximum allowable volume.

Thermo Fisher Scientific

DESCRIPTION Rotor Specifications

Replacement Parts

Tables 1-2 and 1-3 list part numbers of replacement parts available for each Thermo Scientific Superspeed Fixed-Angle Rotor. Figures 1-1 and 1-2 show parts location.

Table 1-2. Parts Identification for the SA-600, SE-12, SM-24 and SS-34 Rotors Catalog Number*

*

Thermo Fisher Scientific

Item

Description

SA-600

SE-12

SM-24

SS-34

-

Rotor Assembly

28500

27004

29017

28020

Rotor Cover Assembly (complete)

46531

--

46528

46504

Rotor Holddown Assembly

46525

--

46525

46525

1

Adapter

28021

28021

28021

28021

2

Cap Screw

63078 (4)

63078 (4)

63078 (4)

63078 (4)

3

Setscrew

60077 (4)

60077 (4)

60077 (4)

60077 (4)

4

Rotor Cover Assembly

12291

27002

29016

28018

5

O-ring (Cover)

60810

61720

60696

60188

6

Cover Sealing Stud

28008

28008

28008

28008

7

Flat Washer (1/4 in)

60751 (2)

60751 (2)

60751 (2)

60751 (2)

8

Lock Washer

63021

63021

63021

63021

9

Cover Stud Top Washer

28010

28010

28010

28010

10

Cover Seat Washer

28011

28011

28011

28011

11

Retaining Ring

60067

60067

60067

60067

12

Cover Seat

28007

28007

28007

28007

13

Cover Clamping Stud

28006

28006

28006

28006

14

O-ring

61616

61616

61616

61616

15

O-ring

60189

60189

60189

60189

-

Extractor Wrench

01031

01031

01031

01031

Number in parentheses indicates quantity required.

Superspeed Fixed-Angle Rotors

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DESCRIPTION Rotor Specifications

Figure 1-1. Parts Location: SA-600, SE-12, SM-24 and SS-34 Rotors

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Thermo Fisher Scientific

DESCRIPTION Rotor Specifications

Table 1-3. Parts Identification for the SA-300 Rotor Item

Description

Catalog Number

-

Rotor Assembly

18100

-

Rotor Cover Assembly, includes:

18122

1

Rotor Cover

18112

2

Rotor Locking Knob

18115

3

Cover Locking Knob

18111

4

O-Ring, Rotor Locking Knob

61616

5

Retaining Ring, Cover Locking Knob

61882

6

Washer, Cover Knob, Top

60751

®

7

O-ring, Cover, Viton

8

O-ring, Cover Knob Shaft, Viton®

91245 ®

67745

9

O-ring, Cover Knob, Viton

10

Washer, Splitlock, SST

63021

11

Washer, Cover Knob Shaft, SST

11921

12

Washer, Cover Knob, Bottom, Nylon

11936

67908

Figure 1-2. Parts of the SA-300 Rotor

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Superspeed Fixed-Angle Rotors

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DESCRIPTION Rotor Specifications

Tubes, Bottles and Adapters

Each Thermo Scientific Superspeed Fixed-Angle Rotor accepts a variety of plastic, glass, and stainless steel tubes and bottles. Special adapters permit the use of tubes and bottles other than those which fit the basic rotor compartment. For an up-to-date list and complete description of all available tubes, bottles, and adapters currently available for use with these rotors, please refer to the most current Product Guide. To order or for information, in the United States, call Thermo Fisher Scientific toll-free 1-866-9THERMO; outside the United States, contact the nearest Thermo Scientific office (see back cover) or your local representative for Thermo Fisher Scientific products. Thermo Scientific information is available on our internet web site at http://www.thermo.com. The chemical compatibility of rotor elements and accessory materials is given in the Appendix. This data is to be used only as a guide when selecting a tube or bottle suited for the sample being used.

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Thermo Fisher Scientific

Chapter 2 OPERATION

Prerun Safety Checks

To ensure safe performance of the rotor, before every run you should: a. read the Safety Information Page in the front of this manual. b. make sure each tube compartment is clean and shows no sign of corrosion. c.

be sure the rotor itself is clean and show no sign of corrosion or cracking. Also, make sure there are no scratches or burrs around the rim of the rotor.

d. check the centrifuge chamber, drive spindle, and tapered mounting surface of the rotor to be sure they are clean and free of scratches and burrs. e. check the chemical compatibility of all materials used (see the Appendix). f.

inspect the rotor cover O-ring and the cover locking knob O-ring for cracks, tears, or abrasions and replace if necessary (see Tables 1-2 , 1-3 and 1-4).

g. make sure the rotor cover is on and properly tightened (paragraph 2-6). h. be sure the proper environment has been selected for operation; for example, controlled ventilation or isolation, if required.

Compartment Loads in Excess of Design Mass

There is a maximum allowable compartment mass established for each superspeed fixed-angle rotor. To prevent rotor failure, the total contents for each compartment, including specimen, tubes, cover, and adapter (if used), should not exceed the figure given in Table 2-1 unless rotor speed is reduced proportionately. Strict adherence to the maximum allowable compartment mass or reduced speed (see below) is required to prevent rotor failure. Observe the WARNING on the Safety Information Page in the front of this manual. The rotor speed is reduced in proportion to the square root of the ratio of the maximum allowable compartment mass to the actual mass of the total load. If the compartment mass is more than that specified for the rotor (see Table 2-1) the reduced speed can be determined by using the formula given below.

Thermo Fisher Scientific

Superspeed Fixed-Angle Rotors

2-1

OPERATION Critical Speed

Table 2-1. Maximum Compartment Mass for Each Rotor

Critical Speed

Rotor

Maximum Compartment Mass (grams)

Maximum Rotor Speed (rpm)

SS-34

115

20,500

SE-12

30

26,000

SM-24

27

20,500

SA-300

115

25,000

SA-600

115

17,000

The critical speed is that speed at which any rotor imbalance will produce a driving frequency equal to the resonant frequency of the rotating system (that is, the rotor and centrifuge drive). At this speed, the rotor may produce large amplitude vibrations which can be felt in the instrument frame. Mass imbalance will contribute to increased vibration intensity at the critical speed. Operation at the critical speed will have a detrimental effect on centrifuge component life and therefore, should be avoided (the critical speed for each superspeed fixed-angle rotor is given in Table 2-2). Observe the CAUTION on the Safety Information Page in the front of this manual.

Table 2-2. Critical Speed Rotor

Thermo Scientific RC-28S* RC-26 PLUS and RC-24 Evolution RC

Thermo Scientific RC-5C PLUS, RC-5C, RC-5B PLUS, RC-5B, RC-6 PLUS, RC-6,

SA-300

1400

1100

SA-600

1200

950

SE-12

1700

1100

SM-24

1300

1100

SS-34

1350

1140

*The SA-300 Rotor can not be used in the RC-28S Centrifuge.

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Superspeed Fixed-Angle Rotors

Thermo Fisher Scientific

OPERATION Critical Speed

Relative Centrifugal Force (RCF) Determination

Relative Centrifugal Force (RCF) refers to the force during cen-trifugation that moves the particulate outward from the center of rotation. This force is proportional to the radial distance and the square of the rotor speed. RCF is determined by the following formula:

when r = the radius in centimeters from the centerline of the rotor to the point in the tube where RCF value is required and rpm= the rotor speed in revolutions per minute Figure 2-1 shows the point of measurement for the minimum, average and maximum radii of a superspeed fixed angle rotor; the illustration on the left is for a sealed bottle used with the maximum allowable fluid volume, and the one on the right shows an uncapped tube with maximum allowable fluid volume. Tables 4-1 through 4-6 (Section 4) provide the RCF value at each radius for all superspeed fixed-angle rotors at speeds from 1000 rpm to the maximum rotor speed (in increments of 500 rpm).

Note The radii values given are the actual rotor specifications; these values do not take the thickness of the tube (bottle or adapter) into consideration.

Figure 2-1. Cross Section of Superspeed Fixed-Angle Rotor Showing Radial Distances If using less than the maximum allowable fluid volume in a tube or a bottle, the minimum and average radius will change depending on the amount of fluid. The maximum radius is a fixed distance that will not change.

Thermo Fisher Scientific

Superspeed Fixed-Angle Rotors

2-3

OPERATION Calculation of Sedimentation Times in Aqueous (Non-Gradient) Solutions

The minimum radius is found by subtracting this distance from the maximum radius. The average radius is simply one-half the sum of the minimum and the maximum radii. The RCF values may now be determined for these radii by using the formula given.

Figure 2-2. Determining the Minimum Radius of a Partially-Filled Tube or Bottle

Calculation of Sedimentation Times in Aqueous (Non-Gradient) Solutions

The time required to sediment a particle in water at 20°C through the maximum rotor path length (that is, the distance between rminimum and rmaximum) can be estimated using the equation:

where: t

= sedimentation time in hours

K

= the clearing factor for the rotor (defined below)

S20, W = the sedimentation coefficient for the particle of interest in water at 20°C as expressed in Svedbergs1

1The sedimentation coefficient (S) in seconds, for a particle in a centrifugal field is defined by the equation S = (dx/dt) [1/(ω2x)]; where dx/dt = sedimentation velocity of the particle in cm/s; w = rotor speed in rad/s; and x = the distance of the particle from the axis of rotation in centimeters. Conventionally, experimentally determined values of sedimentation coefficients are multiplied by 1013 to convert them to Svedberg units (S), so a particle with an experimentally determined sedimentation coefficient of 10-11 seconds is usually referred to in the literature as a "100 S particle." Since the value determined for the sedimentation coefficient is dependent on the density and viscosity of the solution in which centrifugation is performed, values are usually reported for the standard conditions of infinite dilution in water at 20°C, and designated S20, w. 20

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Superspeed Fixed-Angle Rotors

Thermo Fisher Scientific

OPERATION Rotor Loading, Balancing and Sealing

The clearing, or K, factor is defined by the equation:

Where rmaximum and rminimum are the maximum and minimum rotor radii, respectively, and rotor speed is expressed in rpm. K factors for the superspeed fixed-angle rotors for speeds from 1000 rpm to the maximum rpm (in increments of 500 rpm) have been listed in Tables 4-1 through 4-6 (Section 4). Example: The SS-34 Rotor used with unsealed tubes or bottles has a K factor of 381 at the maximum permitted speed (20,500 rpm). If the particles to be sedimented have a sedimentation coefficient of 100 S, the estimated run time required at maximum speed will be:

Note that the calculation assumes particles in water at 20°C. If the suspending medium is denser or more viscous than water, the sedimentation time will be greater.

Rotor Loading, Balancing and Sealing

The rotor can be operated with either a full or less than full complement of tubes as long as the load is properly balanced. Do not operate the rotor unless it is balanced as described below and illustrated in figure 2-3. Operating the rotor out of balance can cause damage to the centrifuge drive assembly. •

The load in each opposing rotor compartment, including adapters (where applicable), tube, and specimen must be properly balanced within five (5) grams. Rotor imbalance can cause damage to the centrifuge drive.

•

When using less than a full complement of tubes, the rotor can be operated at its maximum allowable speed provided opposing pairs of tubes are positioned as shown in figure 2-3.

Note If you are using ULTRACRIMP® tubes in the SA-300 rotor, prepare them for use following the tube filling and tube sealing procedures given in the ULTRACRIMP® Tube Sealing System instruction manual.

Thermo Fisher Scientific

Superspeed Fixed-Angle Rotors

2-5

OPERATION Rotor Loading, Balancing and Sealing

Figure 2-3. Rotor Balancing

Table 2-3. Rotor Balancing Rotor six-place rotor

SA-300

eight-place rotor

SS-34

twelve-place rotor

SE-12, SA-600

twenty-four place rotor

SM-24

Load and seal the SA-300 rotor as follows: 1. Gently load filled tubes into the tube compartments, balancing the rotor as described. If using ULTRACRIMP® tubes in the SA-300, insert a rotor cap (Catalog No. 03538) into each tube compartment that contains a filled tube. The rotor cap will seat against top edge of tube compartment (see figure 2-4).

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Superspeed Fixed-Angle Rotors

Thermo Fisher Scientific

OPERATION Rotor Loading, Balancing and Sealing

2. Check that the locking knob thread is lightly coated with lubricant, Catalog No. 61556.

Note Apply a light coat of lubricant, to the threads of the rotor locking knob after every five or six runs. The lubricant is supplied with the rotor.

3. Inspect the exposed O-rings on the rotor cover. Apply a thin film of vacuum grease if they are dry (Catalog No. 65937).

Note For the SA-300 rotor, a light coat of vacuum grease must be applied to the rotor cover O-ring and the cover locking knob O-ring every five or six runs to ensure the biocontainment seal works. For location of the O-rings, see figure 1-3. Vacuum grease is supplied with the rotor.

4. Place the rotor cover on the rotor. All superspeed fixed-angle rotors are equipped with double locking knobs on the cover. The larger knob secures the cover to the rotor and the smaller knob secures the rotor to the drive spindle. Before installing the rotor in the centrifuge, tighten the larger knob to tighten the cover to the rotor. The cover is tight when you feel a definite stop in the threads and there is no gap between the rotor body and the cover. Observe the CAUTION.

CAUTION Do not operate the rotor without the cover securely locked in place. Failure to do so can cause the cover to become disengaged during centrifugation.

Thermo Fisher Scientific

Superspeed Fixed-Angle Rotors

2-7

OPERATION Rotor Installation

Figure 2-4. Properly Seated ULTRACRIMP® Rotor Cap

Rotor Installation

Before placing the rotor on the tapered spindle of the centrifuge rotor drive, make sure that the rotor centerhole and tapered spindle are clean and free of nicks and scratches. Wipe these surfaces clean before each use. Place the rotor on the tapered spindle and carefully engage the drive pins. CAUTION 1. Failure to load and install the rotor in accordance with the instructions in the rotor operating guide could result in damage to the centifuge. The rotor cover must on and locked in place and the rotor must be locked on the the drive spindle. 2. Use specified Thermo Scientific rotors ONLY. 3. Do not incline or move the instrument while the rotor is spinning. 4. Do not place any object on the instrument or lean on the instrument.

Note If the temperature of the rotor chamber is below room temperature, precool the rotor to the lower temperature before securing the rotor in place. Cooling the rotor will minimize the chance of it seizing to the tapered spindle. Perform the centrifuge run as explained in the centrifuge instruction manual.

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Superspeed Fixed-Angle Rotors

Thermo Fisher Scientific