T-865 Fixed-Angle Ultracentrifuge 52266-15 Instruction Manual March 2010

© 2009 Thermo Fisher Scientific Inc. All rights reserved.

ULTRACRIMP< is either a registered trademark or a trademark of Thermo Fisher Scientific. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.

Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the product operation. This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc. The contents of this document are subject to change without notice. All technical information in this document is for reference purposes only. System configurations and specifications in this document supersede all previous information received by the purchaser. Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or errorfree and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might result from any use of this document, even if the information in the document is followed properly. This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of Sale shall govern all conflicting information between the two documents. Release history: 52266-15 printed in March 2010.

For Research Use Only. Not for use in diagnostic procedures.

T Table of contents Thermo Scientific T-865 Fixed-Angle Ultracentrifuge Rotor ... ii Important Safety Information ... iii Chapter 1

DESCRIPTION ... 1-1 Rotor Description ... 1-2 Rotor Specifications ... 1-2 Accessories ... 1-2

Chapter 2

OPERATION ... 2-1 Prerun Safety Checks ... 2-2 Compartment Loads in Excess of Design Mass ... 2-2 Critical Speed ... 2-3 Rotor Precool ... 2-3 Relative Centrifugal Force (RCF) Determination ... 2-3 Calculation of Sedimentation Time in Aqueous (Non–gradient) Solutions ... 2-7 Chemical Compatibility ... 2-8 Rotor Balancing ... 2-8 Rotor Loading and Sealing ... 2-9 Centrifuge/Rotor Log Book ... 2-11

Chapter 3

CARE and MAINTENANCE ... 3-1 Corrosion ... 3-2 Cleaning ... 3-2 Storage ... 3-4 Overspeed Decal Replacement ... 3-4 Service Decontamination Policy ... 3-5

Chemical Compatibility Chart ...A-1 Contact Information ...B-1

Thermo Scientific

T-865

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P

This manual is a guide for the use of

Thermo Scientific T-865 Fixed-Angle Ultracentrifuge Rotor Data herein has been verified and is believed adequate for the intended use of the rotor. Because failure to follow the recommendations set forth in this manual could produce personal injury or property damage, always follow the recommendations set forth herein. Thermo does not guarantee results and assumes no obligation for the performance of rotors or other products that are not used in accordance with the instructions provided. This publication is not a license to operate under, nor a recommendation to infringe upon, any process patents. Publications prior to the Issue Date of this manual may contain data in apparent conflict with that provided herein. Please consider all data in this manual to be the most current. NOTES, CAUTIONS, and WARNINGS within the text of this manual are used to emphasize important and critical instructions. WARNING informs the operator of a hazard or unsafe practice that could result in personal injury, affect the operator's health, or contaminate the environment. CAUTION informs the operator of an unsafe practice that could result in damage of equipment. NOTE highlights essential information.

CAUTION and WARNING are accompanied by a hazard symbol and appear near the information they correspond to.

© 1998, 1999, 2008, 2010 by Thermo Fisher Scientific

Thermo Scientific

T-865

ii

Preface

Important Safety Information Certain potentially dangerous conditions are inherent to the use of all centrifuge rotors. To ensure safe operation of this rotor, anyone using it should be aware of all safe practices and take all precautions described below and throughout this manual.

WARNING When using radioactive, toxic, or pathogenic materials, be aware of all characteristics of the materials and the hazards associated with them in the event leakage occurs during centrifugation. If leakage does occur, neither the centrifuge nor the rotor can protect you from particles dispersed in the air. To protect yourself, we recommend additional precautions be taken to prevent exposure to these materials, for example, use of controlled ventilation or isolation areas. Always be aware of the possibility of contamination when using radioactive, toxic, or pathogenic materials. Take all necessary precautions and use appropriate decontamination procedures if exposure occurs. Never use any material capable of producing flammable or explosive vapors or creating extreme exothermic reactions. Never exceed the maximum rated speed of the installed rotor; to do so can cause rotor failure. Always reduce (derate) rotor speed as instructed in this manual whenever: • the rotor speed/temperature combination exceeds the solubility of the gradient material and causes it to precipitate. • the compartment load exceeds the maximum allowable compartment load specified (average fluid density greater than 1.2 g/ml). See page 2-2. Failure to reduce rotor speed under these conditions can cause rotor failure..

iii

T-865

Thermo Scientific

Preface

CAUTION Do not expose aluminum rotor components to: strong acids, bases, or alkaline laboratory detergents; liquid chlorine bleach; salts (chlorides) of heavy metals such as cesium, lead, silver, or mercury. Use of these material with aluminum can cause a chemical reaction that initiates corrosion. Do not operate or precool the rotor at the critical speed, as this will have a detrimental effect on centrifuge component life. See pages 2-2 and 2-3. Operate the rotor only when it is symmetrically balanced as described in this manual. Operating the rotor out of balance can cause damage to the centrifuge drive assembly. Rotors with covers must be operated with the cover in position and locked in place. Always maintain the rotor in the recommended manner. The rotor and all accessories must be clean and inspected prior to each run: do not use rotors showing signs of corrosion or cracking. See Chapter 3, Care and Maintenance. Do not autoclave the aluminum rotor cover or expose any aluminum rotor parts to temperatures in excess of 100°C.

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1

DESCRIPTION This manual contains information required to operate and maintain the Thermo Fisher Scientific T-865 Fixed-Angle Ultracentrifuge Rotor.. If you require additional information regarding operation or maintenance, please contact Thermo Fisher Scientific for assistance. In the United States, call Thermo Fisher Scientific toll-free 1-866-9THERMO; outside the United States, contact the nearest Thermo Fisher Scientific office (see back cover) or your local representative for Thermo Fisher Scientific products. Thermo Fisher Scientific product information is available on our internet web site at http:// www.thermo.com.

Contents

• “Rotor Description” on page 1-2 • “Rotor Specifications” on page 1-2 • “Accessories” on page 1-2

Thermo Scientific

T-865

1-1

1 DESCRIPTION Rotor Description

Rotor Description The T-865 is an 8-place fixed-angle rotor that can be used at speeds up to 65 000 rpm.1 The rotor body is machined from a titanium forging for strength and corrosion resistance. The aluminum lid and locking nut are given a blue anodized finish for surface protection.The rotor is tightly sealed during operation by two O-rings - one that fits inside the rotor lid and another around the bottom of the locking nut. The eight tube compartments are bored at a 23.5° angle to the axis of rotation. A disc with alternative black and reflective segments on the bottom of the rotor provides overspeed protection.

Rotor Specifications Table 1-1. Rotor Specifications Rotor Type

Fixed Angle

Maximum Speed

65 000 rpm*

Relative Centrifugal Force (RCF) at Maximum Speed:

*

at rminimum (9.10 cm)

429 459

at raverage (6.47 cm)

305 340

at rmaximum (3.84 cm)

181 222

K Factor at Maximum Speed

51.7

Critical Speed

1200 rpm

Tube Size ( Maximum)

25 mm x 97 mm

Capacity per Tube Compartment (Nominal)

36 ml

Number of Tube Compartment

8

Total Rotor Capacity

288 ml

Maximum Tube Compartment Mass

66 g

Tube Angle

23.5°

Rotor Diameter

19.5 cm (7.75 inch)

Rotor Weight (empty)

8.3 kg (18.3 lb)

With tubes filled with a homogenous solution having an average density of 1.2 g/ml or less.

Accessories a. Accessories Supplied The accessories supplied with the T-865 Rotor, Catalog No. 54305 (rotor and complete accessories), are listed in Table 1-1. Those items indicated by an asterisk (*) are not supplied with Catalog No. 51411, which is a T-865 with basic accessories only. 1

Speed in revolutions per minute (rpm) is related to angular velocity, ω, according to the following:

2π ω = (rpm) ⎛⎝ ------⎞⎠ = ( rpm ) ( 0.10472 ) 60 Where ω = rad/s. All further references to speed in this manual will be designated as rpm.

1-2

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Thermo Scientific

1 DESCRIPTION Accessories

Note In order to seal the ULTRACRIMP< tubes (supplied with Catalog No. 54305) you must have an ULTRACRIMP<Sealing Tool, Catalog No. 03920. To order replacement accessories, telephone 1-800-522-7746 in the United States. Outside the United States, contact the local representative, distributor or agent for Thermo Fisher Scientific products. Be sure to provide the catalog number and description of the part included in Table 1-1, plus the rotor model and serial number. Table 1-2.Accessories Supplied Quantity

*

Catalog Number *

Description

ULTRACRIMP< tubes, Polyallomer, 35 ml (Pkg/25)**

8

03989

8

03996*

1

*

03935

Tube Racks, 4-place (1 pair)

1

*

12826

ULTRACRIMP< Tube Removal Tool

1

51942

Rotor Stand

1

65937

Vacuum Grease

1

61556

Lubricant

1

51345

Overspeed Decal, 65 000 rpm, (extra)

2

64666

Rotor Cover O-Ring, Viton® (extra)

2

64667

Locking Nut O-Ring, Viton® (extra)

1

52392

Rotor Cover Tool

1

52384

Centrifuge/Rotor Log Book

1

52266

Instruction Manual

Rotor Cap

Not supplied with Catalog No. 51411 (basic accessories only).

**

Each package of tubes is supplied with an equal number of tube caps and tube plugs.

b. Optional Multipiece Sealing Assembly If you prefer, you can use the T-865 Rotor with thinwall polyallomer tubes and aluminum sealing cap assemblies. These sealing assemblies consist of a tube plug, tube plug cover, sealing nut, and postfill screw. When the assembly is tightened, the cover crimps the top of the tube to form the seal. To use these assemblies, you must purchase all items separately: the T-865 Rotor with basic accessories only, Catalog No. 51411; a Tool Kit, Catalog No. 52610; the tubes; and the sealing assembly components. Table 1-2 lists the catalog number and description of the tubes and sealing assembly components required, and Table 1-3 lists the parts supplied in the Tool Kit. If you have the tools listed, you need not purchase the kit; or, if necessary, any of the tools listed can be ordered separately by the catalog number given in the table.

Thermo Scientific

T-865

1-3

1 DESCRIPTION Accessories

Table 1-3.Optional Multipiece Sealing Assembly Components Catalog Number

Description

03141

Tubes, 36.0 ml, Polyallomer (Pkg/25)

52572

Sealing Cap Assembly (each); 8 required

Table 1-4.Tool Kit (Catalog No. 52610) for use with Multipiece Sealing Assembly Catalog Number

Description

51834

Tube Cap Vise

52013

Tube Removal Tool

66298

Tube Cap Wrench, 3/4 inch

Note A Delrin< adapter (Catalog No. 00399) is available that fits the tube compartments of the T-865 Rotor. With this adapter, the rotor can also be used with 11.5 ml tubes and 10 ml tubes or bottles to a maximum speed of 30 000 rpm. For ordering information, please see Thermo Fisher Scientific Rotor and Tube Guide on our internet web site at http:// www.thermo.com

c. Rotor Cover Removal Tool The rotor cover of the T-865 Rotor is designed to fit the rotor body tightly to ensure a positive seal; however, because of this tight fit, it is sometimes difficult to remove. If you have difficulty removing the rotor cover, a removal tool is available (Catalog No. 52652; complete instructions for use of the tool are supplied with it).

1-4

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2

OPERATION Contents

• “Prerun Safety Checks” on page 2-2 • “Compartment Loads in Excess of Design Mass” on page 2-2 • “Critical Speed” on page 2-3 • “Rotor Precool” on page 2-3 • “Relative Centrifugal Force (RCF) Determination” on page 2-3 • “Calculation of Sedimentation Time in Aqueous (Non–gradient) Solutions” on page 2-7 • “Chemical Compatibility” on page 2-8 • “Rotor Balancing” on page 2-8 • “Rotor Loading and Sealing” on page 2-9 • “Centrifuge/Rotor Log Book” on page 2-11

Thermo Scientific

T-865

2-1

2 OPERATION Prerun Safety Checks

Prerun Safety Checks To ensure safe performance of the rotor, before every run you should: a. read the Safety Information Page in the front of this manual. b. make sure each tube compartment is clean and that there is no sign of corrosion. c. be sure the rotor itself is clean and shows no sign of corrosion or cracking. Also, make sure there are no scratches or burrs around the rim of the rotor. d. check the centrifuge chamber and drive spindle to be sure that they are clean and free of scratches and burrs. e. verify that the proper overspeed decal is firmly attached to the bottom of the rotor; the decal should have 14 black segments (see page 3-4 for Overspeed Decal Replacement procedure). f.

check the chemical compatibility of all materials used (see Appendix A).

g. inspect the rotor cover O-rings for cracks, tears, or abrasions; replace if necessary. Be sure the O-rings are properly lubricated. h. make sure the rotor cover is on and properly tightened. i.

be sure that the proper environment has been selected for operation; for example, controlled ventilation or isolation, if required.

j.

check the top speed capability of the tube (or bottle) being used. CAUTION When using a tube or bottle assembly other than those supplied by Thermo Fisher Scientific, be sure to check the top speed capability; when in doubt, do a test run for the desired application. If using a Thermo Fisher Scientific tube (or bottle) assembly other than those supplied with the rotor, refer to the Product Guide for the maximum speed. Exceeding the top speed capability of the tube (or bottle) can result in its breakage.

Compartment Loads in Excess of Design Mass The maximum operating speed (65 000 rpm) is based on the recommended design mass that has been established for this rotor, representing the maximum mass that each tube compartment can contain at top speed. The total contents of each compartment (including specimen, tube, rotor cap, tube cap, and tube plug) should not exceed the recommended figure unless the rotor speed is reduced proportionately. (If using optional multipiece sealing assembly, include the weight of the complete assembly.) Strict adherence to the maximum allowable compartment mass or reduced speed is required to prevent rotor failure. Observe the WARNING on the Safety Information Page in the front of this manual. The design mass for each compartment of the T-865 Rotor is 66 grams at 65 000 rpm. This figure is based on the use of a thinwall polyallomer tube filled with a liquid at 1.2 specific gravity, plus all sealing assembly components. If the total compartment mass is greater than 66 grams, use the following formula to determine the reduced speed:

66 g Reduced Speed = 65000 -------------------------------Actual Weight

2-2

T-865

Thermo Scientific

2 OPERATION Critical Speed

Critical Speed The critical speed is that speed at which any rotor imbalance will produce a driving frequency equal to the resonant frequency of the rotating system (that is, the rotor and the centrifuge drive). At this speed, the rotor may produce large amplitude vibrations which can be felt in the centrifuge frame. Mass imbalance will contribute to increased vibration intensity at the critical speed. Avoid operating the rotor at the critical speed, which is approximately 1200 rpm for the T-865 Rotor. Operation at the critical speed will have a detrimental effect on centrifuge component life. Observe the CAUTION on the Safety Information Page in the front of this manual.

Rotor Precool If samples are routinely processed around 4°C or below, the rotor should be stored in a refrigerator or a cold room. If this is not possible, the rotor can be precooled in a Thermo Fisher Scientific Ultracentrifuge. Refer to the ultracentrifuge instruction manual for precooling directions. Be careful not to precool the rotor at its critical speed (read paragraph on page 2-2, Critical Speed).

Relative Centrifugal Force (RCF) Determination Relative centrifugal force (RCF) refers to the force during centrifugation that moves the particulate outward from the center of rotation. This force is proportional to the radial distance and the square of the rotor speed. The RCF value, also known as g force, is determined by the following formula:

rpm RCF = 11.17(r) ⎛⎝ ------------⎞⎠ 1000 when r

2

= the radius in centimeters from the centerline of the rotor to the point in the tube where RCF value is required

and rpm = the rotor speed in revolutions per minute Figure 2-1 shows the minimum, average, and maximum radii of the T-865. Table 2-1 gives the RCF value at each radius at speeds from 20 000 rpm to 65 000 rpm (in increments of 500 rpm). The RCF value at any other speed can be calculated by using the above formula. Note The radii values given are the actual rotor specifications. These values do not take the thickness of the tube into consideration.

Thermo Scientific

T-865

2-3

2 OPERATION Relative Centrifugal Force (RCF) Determination

Table 2-5. T-865 Rotor: RCF Values and K Factors* rmax. 9.10 cm

RCF ravg. 6.47cm

rmin. 3.84 cm

20 000

40 659

28 908

17 157

546

20 500

42 717

30 371

18 026

519

21 000

44 826

31 871

18 916

495

21 500

46 986

33 407

19 827

472

22 000

49 197

34 979

20 760

451

22 500

51 459

36 587

21 714

431

23 000

53 771

38 231

22 690

413

23 500

56 135

39 911

23 688

395

24 000

58 549

41 627

24 706

379

24 500

61 014

43 380

25 746

364

25 000

63 529

45 169

26 808

349

25 500

66 096

46 994

27 891

336

26 000

68 713

48 854

28 996

323

26 500

71 382

50 752

30 121

311

27 000

74 101

52 685

31 269

299

27 500

76 871

54 654

32 438

289

28 000

79 691

56 660

33 628

278

28 500

82 563

58 701

34 840

269

29 000

85 485

60 779

36 073

260

29 500

88 458

62 893

37 327

251

30 000

91 482

65 043

38 604

243

30 500

94 557

67 229

39 901

235

31 000

97 683

69 451

41 220

227

31 500

100 859

71 710

42 560

220

32 000

104 087

74 004

43 922

213

32 500

107 365

76 335

45 306

207

33 000

110 694

78 702

46 710

200

33 500

114 073

81 105

48 136

195

34 000

117 504

83 544

49 584

189

34 500

120 985

86 019

51 053

183

35 000

124 518

88 531

52 544

178

35 500

128 101

91 078

54 056

173

36 000

131 735

93 662

55 589

168

36 500

135 419

96 282

57 144

164

Speed (rpm)

2-4

T-865

K Factors

Thermo Scientific

2 OPERATION Relative Centrifugal Force (RCF) Determination Table 2-5. T-865 Rotor: RCF Values and K Factors* rmax. 9.10 cm

RCF ravg. 6.47cm

rmin. 3.84 cm

37 000

139 155

98 937

58 720

159

37 500

142 941

101 630

60 318

155

38 000

146 778

104 358

61 937

151

38 500

150 666

107 122

63 578

147

39 000

154 605

109 923

65 240

144

39 500

158 595

112 759

66 923

140

40 000

162 635

115 632

68 628

136

40 500

166 726

118 541

70 355

133

41 000

170 869

121 486

72 103

130

41 500

175 062

124 467

73 872

127

42 000

179 305

127 484

75 663

124

42 500

183 600

130 538

77 475

121

43 000

187 945

133 627

79 309

118

43 500

192 342

136 753

81 164

115

44 000

196 788

139 914

83 040

113

44 500

201 286

143 112

84 938

110

45 000

205 835

146 347

86 858

108

45 500

210 435

149 617

88 799

105

46 000

215 085

152 923

90 761

103

46 500

219 786

156 266

92 745

101

47 000

224 538

159 644

94 750

98.8

47 500

229 341

163 059

96 777

96.7

48 000

234 195

166 510

98 825

94.7

48 500

239 099

169 997

100 895

92.8

49 000

244 054

173 520

102 986

90.9

49 500

249 061

177 079

105 098

89.1

50 000

254 118

180 675

107 232

87.3

50 500

259 225

184 306

109 387

85.6

51 000

264 384

187 974

111 564

83.9

51 500

269 593

191 678

113 762

82.3

52 000

274 853

195 418

115 982

80.7

52 500

280 165

199 194

118 223

79.2

53 000

285 526

203 006

120 486

77.7

53 500

290 939

206 855

122 770

76.3

54 000

296 403

210 739

125 075

74.9

Speed (rpm)

Thermo Scientific

K Factors

T-865

2-5

2 OPERATION Relative Centrifugal Force (RCF) Determination

Table 2-5. T-865 Rotor: RCF Values and K Factors* rmax. 9.10 cm

RCF ravg. 6.47cm

rmin. 3.84 cm

54 500

301 917

214 660

127 402

73.5

55 000

307 482

218 616

129 751

72.2

55 500

313 098

222 609

132 121

70.9

56 000

318 765

226 638

134 512

69.6

56 500

324 483

230 704

136 925

68.4

57 000

330 251

234 805

139 359

67.2

57 500

336 070

238 942

141 814

66.0

58 000

341 941

243 116

144 291

64.9

58 500

347 861

247 326

146 790

63.8

59 000

353 833

251 572

149 310

62.7

59 500

359 856

255 854

151 851

61.7

60 000

365 929

260 172

154 414

60.6

60 500

372 053

264 526

156 998

59.6

61 000

378 228

268 916

159 604

58.7

61 500

384 454

273 343

162 231

57.7

62 000

390 731

277 805

164 880

56.8

62 500

397 059

282 304

167 550

55.9

63 000

403 437

286 839

170 242

55.0

63 500

409 866

291 410

172 954

54.1

64 000

416 346

296 018

175 689

53.3

64 500

422 877

300 661

178 445

52.5

65 000

429 459

305 340

181 222

51.7

Speed (rpm)

*

2-6

T-865

K Factors

These values do not take the thickness of the tube into consideration.

Thermo Scientific

2 OPERATION Calculation of Sedimentation Time in Aqueous (Non–gradient) Solutions

Figure 2-1. T-865 Rotor Radii

Calculation of Sedimentation Time in Aqueous (Non–gradient) Solutions The time required to sediment a particle in water at 20°C through the maximum rotor path length (that is, the distance between rminimum and rmaximum) can be calculated using the equation:

K t = ------------S 20 ,w where: t = sedimentation time in hours K = the clearing factor for the rotor (defined on the next page) the sedimentation coefficient for the particle of interest in water at 20°C as expressed in S20, w = Svedbergs1 1

The sedimentation coefficient (S) in seconds, for a particle in a centrifugal field is defined by the equation S = (dx/dt) [1/(ω2x)]; where dx/dt = sedimentation velocity of the particle in cm/s; ω = rotor speed in rad/s; and x = the distance of the particle from the axis of rotation in centimeters. Conventionally, experimentally determined values of sedimentation coefficients are multiplied by 1013 to convert them to Svedberg units (S), so a particle with an experimentally determined sedimentation coefficient of 10-11 seconds is usually referred to in the literature as a "100 S particle." Since the value determined for the sedimentation coefficient is dependent on the density and viscosity of the solution in which centrifugation is performed, values are usually reported for the standard conditions of infinite dilution in water at 20°C, and designated S20, w.

Thermo Scientific

T-865

2-7

2 OPERATION Chemical Compatibility

The clearing (or K) factor is defined by the equation:

r maximum rotor speed 2 K = ( 253000 ) In ⎛ --------------------⎞ ÷ ⎛ -------------------------⎞ ⎝ r minimum⎠ ⎝ 1000 ⎠ Where rmaximum and rminimum are the maximum and minimum rotor radii, respectively, and rotor speed is expressed in rpm. K factors for the T-865 Rotor, at speeds from 20 000 rpm to 65 000 rpm (in increments of 500 rpm), have been listed in Table 2-1. Example: The T-865 Rotor has a K factor of 51.7 at the maximum permitted speed (65 000 rpm). If the particles to be sedimented have a sedimentation coefficient of 10S, the estimated run time required at maximum speed will be:

51.7 t = ---------- 5.17 hours = 5 hours, 10 minutes 10S Note that the calculation assumes particles in water at 20°C. If the suspending medium is denser or more viscous than water, the sedimentation time will be greater.

Chemical Compatibility The critical components of the T-865 Rotor apt to come in contact with solution are: rotor body (titanium), rotor cover assembly (aluminum), rotor cap and tube cap (aluminum), tube plug (Buna N), O-rings (Viton<), plus the material of the tubes (or bottles) being used. The chemical compatibility of rotor elements and accessory materials is given in the Appendix. Because no organized chemical resistance data exists for materials under the stress of centrifugation, this data is intended to be used only as a guide. When in doubt, we recommend pretesting of sample lots.

Rotor Balancing Always balance the rotor according to the following criteria: a. observe the CAUTION on the Safety Information Page in the front of this manual; b. balance pairs of tubes containing fluid of identical specific gravity to within 0.5 gram and place them in opposite tube compartments; c. when using less than a full complement of eight tubes, the rotor can be operated at its maximum allowable speed with two, four, or six samples, provided opposing pairs are positioned as shown in figure 2-2; and d. if one, three, five, or seven samples are to be run, balance the load as above with a dummy tube that contains a solution of the same specific gravity as the sample in the opposing tube compartment.

2-8

T-865

Thermo Scientific

2 OPERATION Rotor Loading and Sealing

Figure 2-2. Compartment Loading

Rotor Loading and Sealing Prepare the ULTRACRIMP® tubes for use by following the tube filling and tube sealing procedures given in the ULTRACRIMP® Tube Sealing System Instruction Manual. Note If using the optional tubes and multipiece sealing assemblies (Catalog No. 03141 and 52572 respectively), seal the tubes and load them in the rotor according to the instructions supplied with the sealing cap assemblies. Load and seal the rotor as follows: 1. Gently place the filled and sealed ULTRACRIMP< tubes in the tube compartments, balancing the rotor as described in previous paragraph. 2. Place a rotor cap (Catalog No. 03996) in each tube compartment that contains a tube. Push the rotor cap into the tube compartment until it is properly seated; when properly seated, the edge of the cap will be approximately flush with the top of the outer edge of the tube compartment. See figure 2-3. CAUTION Do not put a rotor cap in an empty tube compartment. Rotor caps should only be installed in compartments that contain a filled tube.

Thermo Scientific

T-865

2-9

2 OPERATION Rotor Loading and Sealing

Figure 2-3. Properly Seated Rotor Cap 3. Check the locking nut O-ring and rotor cover O-ring for scuffs, cracks or breaks. Replace, if necessary (see Table 1-1 for replacement part catalog numbers). 4. Coat O-rings lightly with vacuum grease, Catalog No. 65937. 5. Check that the locking nut thread is lightly lubricated with grease, Catalog No. 61556. 6. Place the rotor cover (with O-ring) on the rotor body. Finger tighten the locking nut by turning it clockwise. Insert the rotor cover tool through locking nut and continue turning the locking nut clockwise until rotor cover is tightened securely. Remove the rotor cover tool from the locking nut. CAUTION The rotor cover tool must be removed prior to centrifuge run.

7. Carry the rotor carefully, and lower it into the centrifuge chamber slowly and vertically. Be sure it snaps in place on the drive adapter. Check that the rotor is properly seated by pulling it gently in an upward direction and noting a small amount of resistance. Perform the run as explained in the centrifuge instruction manual. The rotor cover is designed to fit the rotor body tightly to ensure a positive seal; however, because of this tight fit, it is sometimes difficult to remove. If you have difficulty removing the cover, a tool is available that is used to easily remove the cover. The catalog number of the rotor cover removal tool is 52652; complete instructions for use of the tool are supplied with it.

2-10

T-865

Thermo Scientific