Heat Sealer Instruction Manual March 2006

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No part of this publication may be reproduced without the prior permission of the copyright owner. Copyright© 2003 Eppendorf AG, Hamburg

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Table of contents Introduction ... 4

2

Safety precautions ... 4

3 3.1 3.2 3.3 3.4

Installation ... 6 Delivery package ... 6 Setting up the device ... 6 Functional units ... 7 Starting up the device ... 7

4 4.1 4.2

Operation ... 8 Sealing ... 8 Removing seal ... 9

5

Maintenance ... 10

6

Troubleshooting ... 11

7

Technical data ... 12

8

Ordering information ... 13

Table of contents

1

3

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1 Introduction / 2 Safety precautions 1

The Heat Sealer offers a convenient method for heat sealing plates made from various types of plastic. The device is designed for low to medium throughput applications.

Introduction / Safety precautions

Pre-set temperature settings enable optimal sealing. Used in conjunction with Eppendorf heat sealing film and foils, the Heat Sealer provides a nearly 100 % seal, preventing sample loss during exposure to high temperatures or for long-term storage.

2 Safety precautions Please read the operating instructions carefully before using the Heat Sealer. If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. The Heat Sealer may only be used by qualified specialists in the research lab. The following instructions must be unconditionally observed: The safety regulations valid for the lab are to be constantly observed when working with the device. In order to avoid damage through condensation, the device may first be switched on 12 hours after being set up. This is also the case when the location of the device is changed. The mains voltage must coincide with the information on the identification plate. The device must be connected to a grounded socket. The device must be switched off and the plug pulled before maintenance work and cleaning are carried out. Wait for the heater plate to cool. The Heat Sealer must stand completely on a stable work surface. Damage to the device can result from using sample plates other than those recommended. Unsuitable plates can be damaged to such an extent that sample material may escape. This is especially important to remember when working with infectious materials. The heater plate is set to approximately 170 °C. Sealed plates can also reach temperatures of over 50 °C very quickly. There is a risk of burns! Coming into contact with heater plate and sample plates should be avoided until temperatures of around 30 °C or less are reached. Do not use materials (plates, foils, films) that are not sufficiently temperature resistant. Do not get your fingers caught between the heater plate and the plates to be sealed. Explosive, flammable and volatile materials may not be sealed with the Heat Sealer. The device may not be operated in rooms at risk of explosion. The appropriate safety regulations should be observed when working with pathogenic material, radioactive materials or other health-damaging materials. In the event of spillage or other contamination of the unit, switch off and disconnect the unit from the mains power supply. Do not attempt to clean the unit until it has thoroughly cooled.

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2 Safety precautions 2

It should be ascertained that no liquid penetrates into the device. Repairs should be carried out by a specialist. Use only original Eppendorf replacement parts.

Safety precautions

Transfer If the device is passed on to someone else, please include the instruction manual. Disposal In case the product is to be disposed of, the relevant legal regulations are to be observed. Information on the disposal of electrical and electronic devices in the European Community The disposal of electrical devices is regulated within the European Community by national regulations based on EU Directive 2002/96/EC on waste electrical and electronic equipment (WEEE). According to these regulations, any devices supplied after 13.08.05 in the business-to-business sphere, to which this product is assigned, may no longer be disposed of in municipal or domestic waste. They are marked with the following symbol to indicate this. As disposal regulations within the EU may vary from country to country, please contact your supplier if necessary.

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3 Installation 3 3.1 Delivery package

Installation

The delivery package contains the following items 1 Heat Sealer 1 Base Plate for 96 well PCR plates 1 Instruction Manual 1 Power lead

3.2 Setting up the device In order to avoid damage caused by condensation, the device may first be switched on 12 hours after setting up. This also applies to a change in location. The Heat Sealer must stand completely stable on the work surface in a safe, dry location away from the edge of the work surface. Space requirement:

Width: 14 cm Depth: 15 cm Height: 33 cm

Mains connection:

An earthed wall socket for the Heat Sealer.

The mains voltage must agree with the information on the name plate. The device must be connected to a grounded socket. The delivery carton should be kept in order to be able to ship the device in case repairs are necessary.

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3 Installation 3 3.3 Functional units

Installation

5

1 2 3

4

1

Heater light

2

Power light

3

Mains switch

4

Heater plate

5

Mains connection socket

0 = off / I = on

3.4 Starting up the device Connect the mains cable to the Heat Sealer and then to the power main. Switch on the mains switch on the side of the unit. If electricity is being supplied to the unit, the green power light will illuminate. If the heater within the unit is receiving power, the amber light will also illuminate. When starting from cold, it is preferable to leave the unit on for 20 to 25 minutes to settle down before carrying out the first sealing operation. On initial power-up, both green and amber lights will appear. After approximately 10 – 15 minutes the amber light will go out. Do not use the Heat Sealer until 20 – 25 minutes have elapsed from power-up. By this time the amber lamp will flicker on and off constantly. This flickering on and off is the normal behaviour exhibited by the amber lamp indicating correct working temperature has been reached. The unit is now up to temperature and ready for sealing. 7

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4 Operation 4 4.1 Sealing

Operation

After installation, follow the procedures set out below: –

Check that the unit is connected to an appropriate power supply and leave for 20 – 25 minutes to settle down to correct operating temperature. The green power lamp should be illuminated and the amber heater lamp flicker on and off.

–

Slide the plate to be sealed on to the platform of the sealer, making sure that it is flat between the two centralizing springs.

–

Deep well plates can be sealed without a base plate. If the plate to be sealed does not have its own support skirt to keep it perfectly flat, place it in an appropriate base plate (PCR Plate 96 should be placed in the metal 96-well base plate supplied with this instrument. PCR Plate 384 has an alternative base plate (see recommended accessories, ordering information).

–

Once the heater plate has reached operating temperature, the amber heater light on the side of the sealer will go off.

–

Place the sealing film over the plate, ensuring that the sealing surface is face down.

–

Holding the handle on the top of the unit, push the heater down to the plate. Once the heater is resting on top of the plate, continue to push down to compress the floating springs which are located behind the plate. Once these are fully compressed (a distance of only 5 mm), this applies a force equivalent to a weight of 10 kg to the surface of the plate.

–

Hold in this position for approximately 2 – 5 seconds (this time will vary significantly depending on the plate material, the surface area and the sealing film being used. An initial time/seal study is strongly recommended for each new type of plate) then turn the plate by 180° and hold for another 2 – 5 seconds (again, an initial time/seal study is strongly recommended). The heater plate is set to approximately 170 °C. Sealed plates can also reach temperatures of over 50 °C very quickly. There is a risk of burns! Coming into contact with heater plate and sample plates should be avoided until temperatures of around 30 °C or less are reached.

–

Return the heater to the top of the track.

–

Remove the plate.

Note: The instrument is fitted with an internal heater with a maximum temperature of approximately 170 °C when ON. Take care to avoid skin contact with the heater plate and to prevent contact between the heater plate and any material which may be damaged or ignited. Foil-backed film may remain hot for a number of seconds after sealing; care should be taken in handling these plates.

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4 Operation 4 4.2 Removing seal Depending on the heat sealing sheet used, it can be removed in a number of ways:

Operation

Eppendorf Peel-it-Lite Heat Sealing Foil (0030 127.668) can be peeled off. Complete removal of the foil can be achieved with the Eppendorf Foil Stripper (0030 127.641) or by hand. If the foil has been removed fully, a new foil sheet can be used to reheat-seal the plate. For removal of the sample, Eppendorf Pierce-it-Lite Heat Sealing Foil (0030 127.676) can be easily pierced with an Eppendorf pipette tip. For long-term storage, a pierced foil seal can be resealed with adhesive PCR Foil (0030 127.471) or PCR Film (0030 127.480) or reheat-sealed with Pierce-it-Lite. Using of Eppendorf Heat Sealing Film (0030 127.650) allows monitoring of the sample, but the seal can only be pierced or peeled off with difficulty.

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5 Maintenance 5

The device may only be opened by a qualified specialist. The device guarantee becomes defunct with unauthorized work on the device!

Maintenance

The device must be switched off and the main plug pulled before maintenance or cleaning is carried out. The heater plate reaches temperatures of more than 50 °C very quickly. There is a danger of burns. Wait until the temperature reaches 30 °C or less. It should be ascertained that no liquid penetrates into the device. Before using any cleaning or decontamination method other than that recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment. You can clean the Heat Sealer cover with a cloth dipped in water, mild lab cleaner or ethanol (methanol or formaldehyde can also be used). Note: Do not use acetone or abrasive cleaners for cleaning! In case of radioactive spillages, Eppendorf recommends that you use a proprietary cleaning agent and follow the manufacturer´s instructions!

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6 Troubleshooting 6

Should you have any recurring problems with this unit, please contact our service department or your local Eppendorf dealer. Return of the device requires its original packing. Cause

Solution

Plates not correctly sealed. Evaporation problems.

–

Sealing time not sufficient.

–

General sealing time 2 – 5 seconds; for appropriate sealing conditions see specifications of used heat sealing film / foil.

–

Sealing not consistent.

–

Turn the plate by 180° after first sealing and hold for another 2 – 5 seconds.

–

Springs behind the heated plate not fully compressed; push the heater head down on to the seal and plate more strongly.

–

Cleaning of heater plate, see chapter 5.

Heater plate impurity.

–

Instrument ON, amber heater – light off (no flickering on and off to top up the heat to the unit).

E.g. film / foil out of place.

Excess-temperature – problem. If the block temperature exceeds 220 °C, thermal cut-out removes power to the heater.

Troubleshooting

Error

Thermal cut-out is non-resettable and the unit should be returned to Eppendorf for repair.

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7 Technical data

Technical data

7 Power supply:

110 V AC (Nominal) 230 V AC (Nominal)

Input frequency:

47 – 63 Hz

Fuses:

T 5 A (T) (110 V) T 3.15 A (T) (230 V)

Power consumption:

Approx. 500 W (110 V) Approx. 400 W (230 V)

Internal heater: Heater plate:

Ambient to 220 °C max. Approx. 170 °C max.

Dimensions:

Width: 14 cm Depth: 15 cm Height: 33 cm

Weight:

3.4 kg

Instrument housing:

Sheet metal

Operating conditions:

10 to 35 °C, 10 % to 80 %, non-condensing

Storage conditions:

– 20 to 40 °C, 10 % to 80 %, non-condensing

Technical specifications subject to change!

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8 Ordering information 8

Desription

Order no. international

Order no. for North America

Heat Sealer, 115 V, incl. Base Plate for 96 well PCR plate

5390 000.016

951023078

Heat Sealer, 230 V, incl. Base Plate for 96 well PCR plate

5390 000.024

Recommended Accessories: Base Plate for 384 well PCR plate Instruction manual

5390 100.029 5390 900.009

951023086

Also available from Eppendorf: Heat Sealing Film 100 sheets PCR Film (adhesive) 100 sheets PCR Foil (adhesive) 100 sheets Pierce-it-Lite 100 sheets Peel-it-Lite 100 sheets Foil Stripper

0030 127.650 0030 127.480 0030 127.471 0030 127.676 0030 127.668 0030 127.641

951023060 951023019 951023001 951023213 951023205 951023043

Eppendorf twin.tec PCR Plate 96, skirted colorless 25 pieces yellow 25 pieces green 25 pieces blue 25 pieces red 25 pieces

0030 128.648 0030 128.656 0030 128.664 0030 128.672 0030 128.680

951020401 951020427 951020443 951020460 951020486

Eppendorf twin.tec PCR Plate 96, semi-skirted colorless 25 pieces yellow 25 pieces green 25 pieces blue 25 pieces red 25 pieces

0030 128.575 0030 128.583 0030 128.591 0030 128.605 0030 128.613

951020303 951020320 951020346 951020362 951020389

Eppendorf twin.tec PCR Plate 384 colorless 25 pieces yellow 25 pieces green 25 pieces blue 25 pieces red 25 pieces

0030 128.508 0030 128.516 0030 128.524 0030 128.532 0030 128.540

951020702 951020711 951020729 951020737 951020745

0030 501.101 0030 505.107

951031801 951032204

0030 501.209 0030 505.204

951032603 951033006

0030 501.306 0030 505.301

951033405 951033600

Eppendorf Plate Deepwell 96/500 µl 20 pieces 120 pieces Eppendorf Plate Deepwell 96/1000 µl 20 pieces 80 pieces Eppendorf Plate/Deepwell 96/2000 µl 20 pieces 80 pieces (also available as sterile or LoBind qualities, and various colors)

Ordering information

Please use only the original accessories recommended by Eppendorf. The function and safety of the device may be negatively affected by the use of accessories or consumables other than those recommended by Eppendorf. We do not honor any warranty or accept any responsibility by damage resulting from such action.

PCR (Polymerase Chain Reaction) is covered by U.S. patents owned by Hoffmann-La Roche.

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Eppendorf Offices ASEAN Eppendorf AG Regional Office in Malaysia Tel. +60 3 8023 2769 Fax +60 3 8023 3720 E-Mail: [email protected]

FRANCE EPPENDORF FRANCE S.A.R.L. Tel. +33 1 30 15 67 40 Fax +33 1 30 15 67 45 E-Mail: [email protected] Internet: www.eppendorf.fr

SPAIN Eppendorf Ibérica S.L. Tel. +34 91 651 76 94 Fax +34 91 651 81 44 E-Mail: [email protected] Internet: www.eppendorf.es

Internet: www.eppendorf.com.my

GERMANY

SWITZERLAND

AUSTRALIA / NEW ZEALAND Eppendorf South Pacific Pty. Ltd. Tel. +61 2 9889 5000 Fax +61 2 9889 5111 E-mail: [email protected] Internet: www.eppendorf.com.au

Eppendorf Vertrieb Deutschland GmbH Tel. +49 2232 418-0 Fax +49 2232 418-155 E-Mail: [email protected] Internet: www.eppendorf.de

Vaudaux-Eppendorf AG Tel. +41 61 482 1414 Fax +41 61 482 1419 E-Mail: [email protected] Internet: www.eppendorf.com

UNITED KINGDOM AUSTRIA Eppendorf AG c/o Schott Austria Tel. +43 1 29017560 Fax +43 1 290175620 E-Mail: [email protected] Internet: www.eppendorf.com

INDIA Eppendorf India Limited Tel. +91 44 52111314 Fax +91 44 52187405 E-Mail: [email protected] Internet: www.eppendorf.co.in

Eppendorf UK Limited Tel. +44 1223 200 440 Fax +44 1223 200 441 E-Mail: [email protected] Internet: www.eppendorf.co.uk

USA ITALY BRAZIL Eppendorf do Brasil Ltda. Tel. +55 11 30 95 93 44 Fax +55 11 30 95 93 40 E-Mail: [email protected] Internet: www.eppendorf.com.br

Eppendorf s.r.l. Tel. +390 2 55 404 1 Fax +390 2 58 013 438 E-Mail: [email protected] Internet: www.eppendorf.it

Eppendorf North America Tel. +1 516 334 7500 Fax +1 516 334 7506 E-Mail: [email protected] Internet: www.eppendorfna.com

JAPAN CANADA Eppendorf Canada Ltd. Tel. +1 905 826 5525 Fax +1 905 826 5424 E-Mail: [email protected] Internet: www.eppendorf.com

Eppendorf Japan Co. Ltd. Tel. +81 3 5825 2363 Fax +81 3 5825 2365 E-Mail: [email protected] Internet: www.eppendorf.jp

NORDIC CHINA Eppendorf AG Tel. +86 21 68760880 Fax +86 21 50815371 E-Mail: [email protected] Internet: www.eppendorf.cn

Eppendorf Nordic Aps Tel. +45 70 22 2970 Fax +45 45 76 7370 E-Mail: [email protected] Internet: www.eppendorf.dk

OTHER COUNTRIES see: www.eppendorf.com/worldwide

In touch with life Your local distributor: www.eppendorf.com/worldwide Eppendorf AG · 22331 Hamburg · Germany · Tel. +49 40 538 01-0 · Fax +49 40 538 01-556 E-Mail: [email protected] Eppendorf North America, Inc. · One Cantiague Road, P.O. Box 1019 · Westbury, N.Y. 11590-0207 USA Tel. +1 516 334 7500 · Toll free phone 800 645 3050 · Fax +1 516 334 7506 · E-Mail: [email protected]

Application Support Europe, International: Tel. +49 1803 666 789 · E-Mail: [email protected] North America: Tel. 800 645 3050 ext. 2258 · E-Mail: [email protected] Asia, Pacific: Tel. +603 8023 2769 · E-Mail: [email protected]

eppendorf is a registered trademark. B 5390 900.009-07/0306 · Printed in Germany

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