Xplorer and Xplorer plus Operating Manual Feb 2012

Copyright© 2012 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copyright owner. eppendorf®, epT.I.P.S.® and Eppendorf Xplorer® are registered trademarks of Eppendorf AG, Hamburg, Germany. Trademarks are not marked in all cases with ™or ®in this manual. Xplorer pipettes are using U.S. Patent Nos. 7,694,592 B2, 8,028,592 B2, 7,674,432 B2, 7,585,468 B2, 7,434,484 B2, 6,778,917, 6,499,365. Further information can be found on the enclosed CD or on our website www.eppendorf.com.

4861 910.011-00/022012

Eppendorf Xplorer® (plus) - Operating manual 1 Table of contents 1

Operating instructions... 6 1.1 1.2

1.3 1.4 2

2.2

2.3 2.4 2.5 2.6

Intended use... 14 Warnings for intended use... 14 Information on product liability... 15

Installation... 16 4.1 4.2 4.3

5

Main illustration... 9 2.1.1 Single-channel... 9 2.1.2 Multi-channel... 10 Display... 10 2.2.1 Header... 11 2.2.2 Status line... 11 2.2.3 Main field... 11 2.2.4 Footer... 12 Delivery package... 12 Features... 13 Warranty... 13 Materials... 13

Safety... 14 3.1 3.2 3.3

4

Using this manual... 6 Danger symbols and danger levels... 6 1.2.1 Hazard icons... 6 1.2.2 Degrees of danger... 6 Symbols used... 6 Glossary... 7

Product description... 9 2.1

3

Table of contents

1

Table of contents

Power supply assembly... 16 Connect rechargeable lithium-polymer battery... 16 Charging the rechargeable battery... 17

Operation... 19 5.1 5.2 5.3 5.4 5.5 5.6 5.7

5.8 5.9 5.10

5.11 5.12 5.13

Switching the pipette on and off... 19 Setting the Date and Time... 19 Principle of operation... 20 Selecting the operating mode... 21 Setting the parameter (edit mode)... 22 Using pipette tips... 24 Tips for correct pipetting... 25 5.7.1 Preparation... 25 5.7.2 Aspirating liquid... 25 5.7.3 Dispensing liquid... 25 Automatic dispensing (Ads dial setting)... 27 Dispensing (Dis dial setting)... 28 Pipetting (Pip dial setting)... 29 5.10.1 Standard pipetting... 29 5.10.2 Reverse pipetting... 30 Pipetting and mixing (P/M dial setting)... 31 Manual pipetting (Man dial setting)... 32 Special operating modes... 33 5.13.1 Multiple aspiration (Aspirate) (Spc dial setting)... 33

3

Eppendorf Xplorer® (plus) - Operating manual

Table of contents

1

5.14 5.15

5.16 5.17

6

Troubleshooting... 49 6.1 6.2

7

Reset... 49 Error search... 49

Maintenance... 51 7.1 7.2

7.3

7.4

7.5

Cleaning... 51 Sterilizing or disinfecting the pipette... 51 7.2.1 Autoclaving... 52 7.2.2 Disinfection... 52 Replacing O-rings... 52 7.3.1 Removing the O-ring... 53 7.3.2 Mounting a new O-ring... 53 Xplorer pipette disassembly and assembly... 53 7.4.1 Single-channel up to 1 000 μL... 53 7.4.2 5 mL and 10 mL single-channel... 56 7.4.3 Multi-channel... 57 Maintenance... 60

8

General information on adjustment... 61

9

Technical data... 63 9.1 9.2 9.3 9.4 9.5

4

5.13.2 Diluting (Spc dial setting)... 34 5.13.3 Sequential dispensing (Spc dial setting)... 36 5.13.4 Reverse pipetting (Spc dial setting)... 37 5.13.5 Sequential pipetting (Spc dial setting)... 38 Program (Prg dial setting)... 39 Editing (Edit dial setting)... 39 5.15.1 Pswrd. protection... 40 5.15.2 Fixed volume... 40 5.15.3 Programm... 41 Pipetting with fixed volume (Fix dial setting)... 42 Option (Opt dial setting)... 42 5.17.1 General help... 42 5.17.2 Volume limit... 43 5.17.3 Counter... 44 5.17.4 Ejector reset... 44 5.17.5 History... 44 5.17.6 Rocker setting... 44 5.17.7 Sound level... 44 5.17.8 Brightness... 44 5.17.9 Adjustment... 45 5.17.10 Language... 47 5.17.11 Personalization... 47 5.17.12 Service... 48 5.17.13 Service interval... 48 5.17.14 Date and Time... 48

Eppendorf Xplorer single-channel... 63 Eppendorf Xplorer multi-channel... 64 Test conditions... 65 Speed table for Xplorer single-channel... 66 Speed table for Xplorer multi-channel... 66

Eppendorf Xplorer® (plus) - Operating manual 1

10 Ordering Information... 67 11 Transport, storage and disposal... 68 Decontamination before shipment... 68 Storage... 68 Disposal... 69

Table of contents

11.1 11.2 11.3

Index... 70

5

Eppendorf Xplorer® (plus) - Operating manual 1

Operating instructions 1

1.1

Operating instructions

Using this manual  Read this operating manual completely before using the device for the first time. Also observe

1

the instructions for use of the accessories.

Operating instructions

 This operating manual is part of the product. thus, must always be easily accessible.  Enclose this operating manual when transferring the device to third parties.  If this manual is lost, please request another one. The current version is available on our website: www.eppendorf.com.

 This operating manual applies to devices with software version 2.01.00 or higher.  The operating manual in additional languages, adjusting instructions and additional information can be found on the Xplorer CD or on our website www.eppendorf.com.

1.2

Danger symbols and danger levels

1.2.1

Hazard icons

1.2.2

Biohazard

Explosion

Electric shock

Toxic substances

Hazard point

Material damage

Degrees of danger The safety instructions in this operating manual indicate the following degrees of danger:

1.3

DANGER

Will lead to severe injuries or death.

WARNING

May lead to severe injuries or death.

CAUTION

May lead to light to moderate injuries.

NOTICE

May lead to material damage.

Symbols used Depiction

Meaning



You are requested to perform an action.

1.

Perform these actions in the sequence described.

2.

•

List.

Text

Terms and key names from the software. References useful information.

6

Eppendorf Xplorer® (plus) - Operating manual 1.4

Glossary

A Air-cushion principle

An air cushion is located between the piston-cylinder system of the pipette and the liquid in the pipette tip. Moving the piston creates a vacuum and may lead to liquid being aspirated into the pipette tip. During the downward movement of the piston, the air cushion is pressed downwards and the liquid in the pipette tip is dispensed. Optimizing the piston stroke ensures that the stroke volume leads to the liquid volume shown in the display.

1 Operating instructions

Pipettes in which the piston is brought into contact with the liquid to be dispensed are defined as positive-displacement pipettes.

B

Biopur

Eppendorf Biopur® is an Eppendorf AG purity level for consumables. In addition to the requirements for standard goods, e.g., precision, accuracy, wetting behavior and leak tightness, Biopur products also meet the highest standards when it comes to sterility and being free of ATP, PCR inhibitors, human and bacterial DNA, pyrogens, DNase and RNase. Goods with the Biopur purity level are inspected and certified by an external laboratory. Lot-specific certificates can be downloaded from our website: www.eppendorf.com.

Blow-out

Movement of the piston that dispenses the remaining liquid in the pipette tip after the pipetting step has been completed. When using normal pipetting, the liquid blow-out is part of the dispensing volume. When using reverse pipetting, blow-out is not part of the dispensing volume.

C Cycle

Together, the piston movement upward (liquid aspiration) and piston movement downward (liquid dispensing) form a cycle.

F Free jet dispensing

Dispensing steps without a pipette tip touching tube wall. If a drip forms on the pipette tip after free jet dispensing, this drip always belongs to the next dispensing step. We recommend completing a dispensing series using only the free jet method or wall dispensing method. Complete the reverse stroke in accordance with the dispensing series using either the free jet or wall dispensing method. Errors listed in the technical data were determined using the wall dispensing method.

I Increment

Step size or resolution. For example, the volume can be increased or decreased in 0.01 mL increments for the volume selection on a 10 mL Xplorer pipette.

ISO 8655

The ISO 8655 standard defines, among other factors, limiting values (systematic error [accuracy] and random error [precision]) and the test procedure for dispensing devices.

M Multivolume Nominal volume

The total of the remaining stroke and the reverse stroke.

N

The maximum dispensing volume of a pipette. The nominal volume is also used as the name of a pipette. The term "nominal volume" comes from the ISO 8655 standard.

P

PCR clean

PCR clean is an Eppendorf AG purity level for consumables. In addition to the requirements for standard goods, e.g., precision, accuracy, wetting behavior and leak tightness, products with the PCR clean purity level also meet the highest standards when it comes to sterility and being free of human DNA, DNase, RNase and PCR inhibitors. Goods with the PCR clean purity level are inspected and certified by an external laboratory. Lot-specific certificates can be downloaded from our website: www.eppendorf.com.

PhysioCare Concept®

Products that carry the Eppendorf PhysioCare Concept logo feature optimized ergonomics and make operation as simple as possible, even with complex dispensing tasks. PhysioCare products can be easily serviced and cleaned. They are made of extremely light materials and require minimal operating force.

7

Eppendorf Xplorer® (plus) - Operating manual R Rechargeable lithium-polymer battery

Operating instructions

1

This rechargeable battery is a special version of the rechargeable lithium-ion battery. Like rechargeable lithium-ion batteries, rechargeable lithium-polymer-batteries have a very high charging capacity and a long service life. The special encapsulation of the rechargeable lithium-polymer-battery makes it lighter than rechargeable lithium-ion batteries.

Remaining stroke

After dispensing steps have been completed, any small quantities of residual liquid as safety reserves; this liquid will be dispensed as the remaining stroke. You can discard the liquid of the remaining stroke or reuse it.

Reverse stroke

After aspiration, the piston is moved into a defined position. Liquid is dispensed during this piston movement. The reverse stroke is not a dispensing step.

S Sterile

Sterile is an Eppendorf AG purity level for consumables. In addition to the requirements for standard goods, e.g., precision, accuracy, wetting behavior and leak tightness, products with the Sterile purity level also meet the highest standards when it comes to sterility and being pyrogen-free.

V

Vapor pressure

This term refers to the pressure exerted by the vapor of a material (solid or liquid) in an enclosed container. The vapor is in equilibrium with the solid or liquid phase of the material. The vapor pressure increases when the temperature increases. Each pure liquid has a vapor pressure of 1013 hPa (mbar) at boiling point. Volume errors caused by high vapor pressure can be reduced by prewetting the tip.

Viscosity

Viscosity describes the viscidity of liquids and suspensions. Dynamic or absolute viscosity is now usually given in Pa · s or mPa · s. The unit P or cP is frequently found in older literature. 1 mPa · s corresponds to 1 cP. At room temperature a 50% glycerin solution has a viscosity of approx. 6 mPa · s. As the glycerin concentration increases, viscosity increases considerably. Absolutely anhydrous glycerin has a viscosity of approx. 1480 mPa · s at room temperature.

W

Wall dispensing

8

Liquid dispensing during which the pipette tip touches the tube inner wall. We recommend completing a dispensing series using only the free jet method or wall dispensing method.

Eppendorf Xplorer® (plus) - Operating manual Product description Product description

Main illustration

2.1.1

Single-channel 1 2 3 4 Ads

P/

M

F ix

Dis Pip

Man Opt

2.1

Sp c Prg it Ed

2 2

5

USB port

2 3 4

Only for Eppendorf service. ON switch Reset key Connector socket

5

For connecting the charging plug of an Eppendorf Xplorer power supply. Selection dial

6

For setting an operating mode. Charging contacts

7

Gold-plated contacts for loading and storage in a series 4880 charger stand or charger carousel. Backlit color display

8

Switch the display off when it is not in use. The display automatically switches back on when the pipette is moved. Softkeys

9

The assignment of the respective softkey is shown in the display footer. Rocker

6 7 8 9 10

12345

11

12

13 14

2 Product description

1

You can either press the rocker up or down. The rocker can either be used to trigger the piston movement or to scroll through the menu and change the values of parameters. The color of the rocker is different for the various volumes and matches the color of the trays for the suitable epT.I.P.S. pipette tips. 10 Ejector Moves the ejector sleeve and ejects the pipette tip. After a filled pipette tip has been ejected, the piston returns to the basic position after a slight delay. This option can be switched off (see p. 42). 11 Serial number 12 Ejector sleeve The ejector sleeve is marked with the nominal volume of the pipette. 13 Spring-loaded tip cone The spring loading action optimizes the force required for attaching and ejecting tips. The spring loading action is available for all Xplorer pipettes up to 1 000 μL. 14 Pipette tip The Xplorer pipettes may only be used with the suitable pipette tip fitted. We recommend using epT.I.P.S.

9

Eppendorf Xplorer® (plus) - Operating manual 2.1.2

Multi-channel A description of the upper part can be found in the main illustration of the single-channel pipette (see p. 9). 1 Lever The lever is used to release the multi-channel lower part. 2 Multi-channel lower part The multi-channel lower part is autoclavable. The multi-channel lower part is freely rotatable. It is not detached when rotated. The outer channels are marked with the numbers 1 and 8 (or 12).

2 Product description

The multi-channel version has a piston for each channel so that less than 8 or 12 tips can also be mounted. You can open the multi-channel lower part in order to exchange or remove individual channels. 3 Latches, right and left

6

Unlock the cover plate (6) and internal ejector rail. The 1 200 μL multi-channel lower part can be opened with a pin (see p. 58). 4 Spring-loaded tip cones for 10 μL, 100 μL and 300 μL

1 2

The spring loading action optimizes the force required for attaching and ejecting tips. 5 Pipette tips

3 4

We recommend using epT.I.P.S. 6 Cover plate for 10 μL, 100 μL and 300 μL Removable cover plate with internal ejector rail. The cover plate is opposite the side with the lever (1).

5

2.2

Display The brightness of the display can be changed in the options (see p. 42). The display dims during usage intervals and switches off after a prolonged usage interval. Move the Xplorer pipette to switch the display back on. 1 2

3

4

10

1

Header

2

Status line

3

Main field

4

Footer

Eppendorf Xplorer® (plus) - Operating manual 2.2.1

Header

Adjustment

Hint!

The adjustment can be changed in the options. The adjustment is described separately (see p. 61). Detailed information can be found in a separate document on the CD and on our website: www.eppendorf.com. If you have changed the factory settings, the symbol will be displayed to the left of the header. Additional symbols to the right indicate which adjustment is selected in the options. 1-point adjust.

2

Single-point adjustment by the user. 2-point adjust.

Product description

Two-point adjustment by the user. 3-point adjust. Three-point adjustment by the user. Glycerol 50% Adjustment to liquid type 50 % glycerol. Ethanol 75% Adjustment to liquid type 75 % ethanol. Altitude Adjustment to an altitude other than 0 m above sea level. epTIPS long Adjustment to an epT.I.P.S. long. Volume The selected volume for the acoustic feedback can be set in the options (see p. 42). If the volume has been set to 0, no loudspeaker symbol is displayed. Battery Information on the charging capacity of the Li-polymer battery Rechargeable battery fully charged. Rechargeable battery partially discharged. Rechargeable battery almost discharged. Rechargeable battery must be charged (see p. 17). 2.2.2

Status line The selected operating mode is displayed here during operation. In edit mode, the name of the parameter to be edited is displayed.

2.2.3

Main field All parameters of the operating mode are displayed simultaneously during dispensing. A black arrow indicates the direction of the next piston movement. In edit mode, the parameter to be edited is marked in red. Some of the dispensing parameters in the main field include:

• Dispensing volume • Speed: 8 speeds for aspiration and dispensing • Counter: Number of conducted dispensing, optional setting in the Pip mode

Hint!

An explanation of all dispensing parameters can be found in the "Setting parameters" chapter (see p. 22).

11

Eppendorf Xplorer® (plus) - Operating manual 2.2.4

Footer Assignment of the softkeys with variable functions. Some of the softkeys in the footer are:

Edit Next End Help History

Open the edit mode. Select next parameter. Exit the edit or help mode. Show help. Display the last dispensing settings of the accessed mode (see p. 44).

2 Product description

Hint!

2.3

The right softkey can be assigned to Help or History. If the right Help softkey is activated, the softkey can be used to display information on the selected operating mode. If the History softkey is activated, it can be used to call the most recently executed dispensings. The assignment of the softkey can be defined in the options (Opt). The softkey is assigned to Help when the Xplorer is delivered.

Delivery package Quantity

Description

1

Eppendorf Xplorer

1

Rechargeable lithium-polymer battery

1

Power supply with power plug adapters

1

English/German operating manual Additional languages are available on the CD.

1

CD with operating manual in several languages, ordering information and additional documents

1

Short instructions in English Additional languages are available on the CD.

1

Locking ring for deactivating the spring loading action for  1 000 μL single-channel Eppendorf Xplorer pipettes

1

For multi-channel pipettes from 100 μL upwards: multi-channel tool for cutting the O-rings on the tip cones

12

1

Unlocking tool for opening the 1 200 μL multi-channel lower part

1

Pipette wrench for opening the lower part on 5 mL and 10 mL single-channel pipettes

2 or 3

Locking clip for lifting the spring loading on 8 or 12 channel lower parts with 10 μL, 100 μL and 300 μL nominal volumes.

Eppendorf Xplorer® (plus) - Operating manual 2.4

Features The Xplorer pipettes are motorized and electronically controlled piston-stroke pipettes designed to aspirate and dispense liquid volumes according to the air-displacement principle. The Xplorer pipette family includes single-channel and multi-channel pipettes (8 or 12 channels). Depending on the Xplorer pipette, you can dispense volumes ranging from 0.5 μL to 10 mL. The liquid is aspirated into a pipette tip which is attached to the Xplorer pipette. The main control element of the Xplorer pipette is a rocker. The color of the rocker differs depending on the volume range of the Xplorer pipette. It has different functions during dispensing operations and in the dispensing parameter editing mode. The rocker can either be pressed up or down.

2

The lower parts of the Xplorer pipettes are autoclavable. For all Xplorer pipettes from 10 μL to 1 000 μL nominal volumes, the tip cone responds with spring-loading action when a pipette tip is attached.

Warranty

Product description

2.5

In case of warranty claims, contact your local Eppendorf contractual partner. No warranty is provided for any damage due to misuse or if the upper part has been opened by unauthorized persons. The rechargeable lithium-polymer battery and all other wear parts are excluded from the warranty.

2.6

Materials Aggressive substances may damage the pipette, pipette tips and accessories. NOTICE!

 Check the chemical resistance before using organic solvents or aggressive chemicals.  Observe the cleaning instructions (see Cleaning on p. 51). Components of the Xplorer pipette that can be accessed by the user are made of the following materials: Component

Material

External surfaces of the upper part

• Improved polypropylene (PP) • Polycarbonate (PC) • Polycarbonate (PC), coated • Polycarbonate (PC), dyed • Gold plated

Exterior and interior of lower parts

• Improved polypropylene (PP) • Polyvinylidene fluoride (PVDF) • Polyetherimide (PEI) • Polyphenylene sulfide (PPS) • Polyetheretherketone (PEEK) • Polytetrafluoroethylene (PTFE) • Ethylene propylene diene rubber (EPDM) • Silicone • Steel (stainless steel and spring steel)

Pipette tip

Material

epT.I.P.S.

Polypropylene (PP)

epDualfilter T.I.P.S. filter

Polyethylene (PE)

13

Eppendorf Xplorer® (plus) - Operating manual 3 3

3.1

Safety Safety

Intended use The Xplorer pipette is a lab device intended for dispensing liquids in the volume range from 0.5 μL to 10 mL, in combination with matching pipette tips. In vivo applications (applications in or on the human body) are not permitted. The Xplorer pipette may only be operated by trained specialist staff. All users must have read the operating manual carefully and familiarized themselves with the device's mode of operation.

3.2

Warnings for intended use Risk of explosion.

DANGER!

3

 Do not operate the device in areas where work is completed with explosive substances.  Do not use this device to process any explosive or highly reactive substances.  Do not use this device to process any substances which could create an explosive atmosphere.

Safety

Damage to health due to handling infectious liquids and pathogenic germs.

 When handling infectious liquids and pathogenic germs, observe the national regulations, the WARNING!

biological security level of your laboratory and the material safety data sheets and the manufacturer's application notes.

 Wear personal protective equipment.  Follow the instructions regarding hygiene, cleaning and decontamination.  For complete instructions regarding the handling of germs or biological material of risk group II or higher, please refer to the "Laboratory Biosafety Manual" (Source: World Health Organization, current edition of the Laboratory Biosafety Manual). Damage to health due to toxic, radioactive or aggressive chemicals.

WARNING!

 Wear personal protective equipment (PPE).  Observe the national regulations for handling these substances.  Observe the material safety data sheets and the manufacturer's application notes. Personal injury caused by the incorrect handling of the rechargeable battery.

WARNING!

 Do not disassemble or modify the rechargeable battery.  Never pierce, crush or throw the rechargeable battery.  Only use the rechargeable battery in the Xplorer pipette.  Do not touch leaky rechargeable batteries.  Do not use any damaged rechargeable batteries.  Dispose of damaged rechargeable batteries in accordance with legal regulations. Danger to people from careless use.

CAUTION!

14

 Never point the opening of a Xplorer pipette fitted with pipette tip at yourself or anyone else.  Only initiate dispensing if it is safe to do so.  For all dispensing tasks, make sure that you are not endangering yourself or anyone else.

Eppendorf Xplorer® (plus) - Operating manual Poor safety due to incorrect accessories and spare parts.

CAUTION!

The use of accessories and spare parts other than those recommended by Eppendorf may impair the safety, functioning and precision of the device. Eppendorf cannot be held liable or accept any liability for damage resulting from the use of incorrect or non-recommended accessories and spare parts or from the improper use of such equipment.

 Only use accessories and original spare parts recommended by Eppendorf. Damage to device from missing pipette tips.

 Only use the Xplorer pipette with fitted pipette tips. NOTICE! Carry-over, contamination and incorrect dispensing results due to the incorrect use of pipette tips. NOTICE!

The pipette tips are for single use only. Prolonged use can have a negative impact on dispensing tasks.

3

 Use the pipette tips only once.  Do not use autoclaved ep Dualfilter T.I.P.S. for dispensing.

Safety

Incorrect dispensing volume with special liquids and from temperature differences. NOTICE!

Solutions which differ greatly from water in terms of their physical data, or temperature differences between the pipette, pipette tip and liquid, can result in incorrect dispensing volumes.

 Avoid temperature differences between pipette, pipette tip and liquid.  Check the dispensing volume and make sure that you can affirm all the questions listed in the general information. Damage to device from penetration of liquids.

NOTICE!

 Do not deposit the Xplorer pipette with a filled pipette tip.  Do not allow any liquids to penetrate the inside of the housing.  If liquid has penetrated the inside of the housing, have the upper part of the Xplorer pipette repaired only by service partners of Eppendorf AG. Contact your responsible sales office before returning a product.

3.3

Information on product liability In the following cases, the designated protection of the device may be compromised. Liability for any resulting property damage or personal injury is then transferred to the operator:

• The device is not used in accordance with the operating manual. • The device is used outside of its intended use. • The device is used with accessories or consumables which are not recommended by Eppendorf.

• The device is maintained or repaired by people not authorized by Eppendorf. • The user makes unauthorized changes to the device.

15

Eppendorf Xplorer® (plus) - Operating manual 4 4

4.1

Installation Installation

Power supply assembly 1 Power plug adapters

1 a

a EU c

b

d

b United Kingdom c USA

GS

N 1388 LEI-4 MADE IN CHINA

UL I.T.E. POWER SUPPLY MODEL NO: INPUT: 100–240 V 50/60 Hz 0.5 A OUTPUT: 5 V = 1.0 A Approval no.

IIIIIII

d Australia 2 Power supply 3 Charging plug

C

IIIIIIIIIIIIIIIIIIII IIIIIIIIIIIIIIIIIIII

3

2

 Fit the power plug adapter required for your power supply into the opening of the power supply. In the event of any doubt which power plug adapter should be used for the power supply unit, you should ask an electrician for advice.

4 4.2

Connect rechargeable lithium-polymer battery The lithium polymer battery is already inserted in the battery compartment upon delivery.

Installation

1. Open the rechargeable battery compartment cover.

2. Connect the plug of the rechargeable lithium polymer battery to the connector socket. 3. Close the rechargeable battery compartment cover. On the Xplorer plus pipette, you will then be prompted to set the date and time (see p. 19).

16

Eppendorf Xplorer® (plus) - Operating manual 4.3

Charging the rechargeable battery Incorrect or damaged power supplies can lead to severe person injury or damage to the device. WARNING!

Incorrect or damaged power supplies can cause electric shock or cause the device to overheat, catch fire, melt, short circuit or cause similar damage.

 Only use the supplied power supply for charging the device. The correct power supply unit is indicated via the Eppendorf logo and the device name on the power supply.

 Do not use a damaged power supply. Loss of full rechargeable battery charging capacity if charged incorrectly. NOTICE!

The supplied rechargeable battery is not charged completely. If the rechargeable battery is not charged completely before it is used for the first time, its charging capacity will remain permanently greatly reduced. The rechargeable battery will only achieve its full capacity after a few discharge/charge cycles.

 Charge the rechargeable battery for at least three hours before it is used for the first time.  Do not charge the rechargeable battery in a hot location (>60°C).  Only charge the rechargeable battery using the supplied power supply.

Hint!

For identification purposes, the power supply contains a sticker with the name of the compatible devices. The power supply is designed for the 100 V- 240 V range.

4

4986 603.005

Multipette stream/Xstream Repeater ® stream/Xstream Xplorer

Installation

®

TM

Recharging the rechargeable battery 1. Insert the assembled power supply (order number 4986 603.005) into a power outlet. 2. In the Xplorer pipette check whether a rechargeable battery is inserted and connected. 3. Insert the charging plug of the power supply into the connector socket of the Xplorer pipette. The display briefly shows the connection with the power supply. The charging process is indicated by a flashing rechargeable battery symbol in the display. If the rechargeable battery is almost completely discharged, the display will only switch on after a slight delay. 4. Switch on the ON switch to observe the charging process. The pipette can be charged in both switch settings. 5. Charge the rechargeable battery for at least three hours. The rechargeable battery will only achieve its full capacity after several discharge/charge cycles.

17

Eppendorf Xplorer® (plus) - Operating manual

CD

A 4880 series charger stand or charger carousel can also be used to store and charge single-channel and multi-channel Xplorer pipettes (see Ordering information on the Xplorer CD).

4 Installation

4880 charging stand

18

4880 charging carousel

Eppendorf Xplorer® (plus) - Operating manual 5 5 5

Operation

Switching the pipette on and off

Operation

5.1

Operation

When not in use, the switched on pipette automatically switches to the standby mode after a period of time. The pipette automatically switches back on when it is moved.

Hint!

Power on On

 Switch on the ON switch to turn on the pipette.

Power off On

5.2

 Switch off the ON switch to turn off the pipette.

Setting the Date and Time Only available for the Xplorer plus pipettes Set the Date and Time parameters during the initial start-up. Requirement

• Battery connected (see p. 16). • Battery charged (see p. 17). • Pipette switched on (see p. 19). 1. Select Date or Time using the rocker. 2. Press the Select softkey. 3. Change the value using the rocker. 4. Press the Next softkey to change the field. 5. Press the End softkey to end the entry. 6. Change the second parameter in the same way. 7. Press the End softkey to end the entry.

Hint!

To change the date and time at a later time, change the Date and Time parameter in the options (see p. 48).

19

Eppendorf Xplorer® (plus) - Operating manual 5

5.3

Principle of operation

Operation

Every Xplorer pipette is equipped with various operating modes and a primary mode for configuring the Xplorer pipette. The adjustable parameters and operational procedures differ in the operating modes. During operation, the piston in the pipette is moved according to how the rocker is actuated. When you press the rocker up, the piston in the pipette moves up. Liquid is aspirated into the attached pipette tip. When you press the rocker down, the piston in the pipette moves down. The liquid is dispensed into the pipette tip. Press the Edit softkey to open the edit mode of the selected operating mode. Here, you can use the rocker to change the parameter that is outlined and marked red.

Hint!

Special operating sequences with an electric pipette are possible with dispensing with the rocker. For example, you can cancel a piston movement in the Dis, Pip and P/M modes at any time by pressing the rocker in the other direction. You can cancel the piston movement in the Ads and Man modes by releasing the depressed rocker. After the piston has been stopped, you can continue the aspiration or dispensing process by pressing the rocker in the corresponding direction. If the piston is in the basic position, you can repeat the blow-out (Blow) at any time by pressing the rocker down.

Hint!

20

The pipette software is available in several languages. To change the language, change the Language parameter in the options (see p. 47).