Legend MACH 1.6/R Instruction Manual Dec 2003

How to use this manual Use this manual to get acquainted with your centrifuge and its accessories. This manual helps you to avoid inappropriate handling. Make sure to keep it close to the centrifuge. A manual that is not kept handy cannot provide protection against improper handling and thus against damage to persons and objects. The manual comprises chapters on • Safety regulations • Instrument description • Transportation and hook-up • Rotor program and accessories • Use of the centrifuge • Maintenance and care • Troubleshooting • Technical data • Index

Overleaf you will find a graphic representation of the control panel with a survey of the most important functions

Please fold out

(only for instruments with refrigeration unit)

deceleration profiles speed/RCF

temperature run time

Quick run

open lid stop start

Bucket selection

rpm/RCF switch key display

Set value input

Before switching on the centrifuge please read this manual

The mains switch is located on the right-hand side panel. Pressed up = ON I Pressed down = OFF 0

Control panel of the Legend™MACH 1.6 /R Display panels Deceleration profiles (1= slowest ... 3= fastest) permanent display: deceleration profile last set 1- 3 Speed / RCF run: end: lid open: (before start) lid closed: error code:

start : stop : open lid: quick run:

normal start of the centrifuge manual stop of a run open lid (possible only with the instrument switched on) short-term operation of the centrifuge as long as key remains pressed

current value of speed or RCF after activation of switch key "End" "OPEN" "Lift Lid" (if lid is not automatically lifted off)

rpm/RCF switch: switching between rpm and RCF display bucket set.: Setting of the bucket number deceleration profiles: switching the deceleration profiles

"0" with flashing point (rotor not yet identified) will flash in display

Adjusting knobs

Run time time selection: - remaining run time to 0 continuous operation (hld): - run time passed (in hours, minutes) quick run: - run time passed (as long as button is held; in minutes and seconds)

Temperature* run:

Keys

current sample temperature in °C (in temperature equilibrium)

* only for instruments with refrigeration unit

Set value input:

setting the respective set values. Short pressing of one of the adjusting knobs effects switching from present to set value on the set value display, signalised by flashing display.

Error codes (troubleshooting see chapter "Troubleshooting"): E-19: Unpermissible rotor E-20: Rotor not identified E-29: Motor or rotor blocked E-31: Overtemperature in motor E-32: Overtemperature in electronic E-33: Excessive pressure in the refrigeration unit E-34: Overvoltage E-35: Overcurrent Lift Lid : Smoothly lift the lid rotor: set speed higher than permissible speed of the rotor bAL : unbalance

Contents

Contents For your safety... 3

Operation ... 35

Proper use... 3 Improper use ... 3 Centrifuging hazardous materials ... 3 Handling the centrifuge ... 4 Conformity to current standards... 5 Safety instructions in this manual ... 5

Switching on the centrifuge ... 35 Actuating the lid... 35 Opening the lid ... 35 Closing the lid ... 35 Installing the rotor... 36 Loading the rotor ... 37 Maximum loading ... 37 Filling the centrifuge tubes ... 38 Maximum permissible load difference ... 38 Inserting the centrifuge tubes ... 39 Entering parameters... 41 Deceleration curves ... 41 Switching from speed to RCF display ... 41 Bucket selection for swinging bucket rotors... 41 Selecting speed... 42 Entering the RCF value... 42 More about the RCF value ... 43 Selecting run time ... 43 Run time selection ... 43 Continuous operation ... 44 Extended time mode... 44 Selecting the temperature ... 45 Starting the centrifuge ... 45 Unbalance display... 46

The Legend™ MACH 1.6 / R ... 7 Description ... 7 Safety systems... 8 Function and features ... 9

Before use... 13 Centrifuge transport and installation ... 13 Proper location ... 13 Main connection ... 14

Rotors and accessories ... 15 Rotors for the Legend™ MACH 1.6 ... 16 Rotors for the Legend™ MACH 1.6 R ... 19 Handling the rotor... 27 Aerosol-tight operation ... 30 Checking of aerosol-tight bio-containment... 34

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Contents Changing the settings during the run ... 46 Stopping the centrifuge ... 46 Stopping with preset run time ... 46 Stopping with continuous operation ... 46 Temperature control during standby ... 47 ”Quick Run” ... 47 Removing the rotor... 48 Audible alarm ... 48 Turning off the centrifuge ... 49

Maintenance and care ... 51 Maintenance to be performed by the customer ... 51 Cleaning... 51 Disinfection ... 52 Decontamination... 54 Autoclaving ... 54 The KENDRO service offer ... 55 Warranty conditions... 55

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Troubleshooting ... 57 Emergency lid release... 57 Error troubleshooting... 59 Contacting Kendro Service... 68

Technical Data ... 69 Electrical connections / fuses ... 71

Appendix ... 73 Deceleration profiles... 73 Speed/RCF diagrams... 79

Index ... 85

For your safety

For your safety ®

Proper use

The SORVALL centrifuges are manufactured according to current technical standards and regulations. Nonetheless, centrifuges may pose danger to individuals and surrounding if

The centrifuge is designed to separate liquidsuspended materials having different densities and particle size, respectively (maximum sample density is 1.2 g/cm³ {ml} at maximum speed).

• they are not used as designed

Improper use

• they are operated by untrained personnel • their design is improperly changed • the safety instructions are not followed Therefore, personnel involved with operation and maintenance of the centrifuge must read and follow the safety instructions. In addition, the pertinent regulations for prevention of accidents must be strictly followed. This manual is an integral part of the centrifuge assembly and must be kept close at hand at all times. When damages to the power cord or at casing are noticed the centrifuge must to be set out of operation!

During a run, a safety zone of 30 cm around the centrifuge must be maintained where neither persons nor hazardous materials may be present. The centrifuge may cause harm to its user or other persons or may damage goods if safety measures are not followed:

Centrifuging hazardous materials • The centrifuge is neither made inert, nor is it explosion-proof. Therefore never use the centrifuge in an explosion-prone environment. • Do not centrifuge explosive or flammable substances. The same holds for substances prone to react violently with each other.

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For your safety • Do not centrifuge toxic or radioactive substances or pathogenic microorganisms without suitable safety systems. If microbiological samples of risk group II (according to "Laboratory Bio-safety Manual" of WHO) are being centrifuged, aerosol-tight bio-seals have to be used. For materials in a higher risk group, more than one precaution is required.

• You may use the centrifuge only with a properly loaded rotor. You must not overload the rotor. • Strictly follow the rules and regulations for cleaning and disinfection • If the rotor or the rotor lid shows signs of corrosion or wear, you must stop using it. • Never open the lid manually if the rotor is still turning.

• Should toxins or pathogenic substances enter the centrifuge or its parts, you must perform appropriate procedures for disinfection (see "Maintenance and care – Disinfection").

• You may use the emergency lid release only in case of emergency, e.g. during an interruption of power supply (see chapter "Troubleshooting").

• Strongly corrosive substances that may cause damage to materials and reduce the mechanical strength of the rotor may be centrifuged only inside protective tubes.

• Never use the centrifuge if the front panel has been partially or totally removed.

Handling the centrifuge • Use only original accessories for the centrifuge. The only exceptions are common glass or plastic centrifuge tubes if they are approved for the rotor speed and RCF values. • Never use the centrifuge unless the rotor is properly installed.

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• Never use the centrifuge with an opened lid.

• Changes in mechanical or electrical components of the centrifuge may only be carried out by individuals authorized by Kendro Laboratory Products.

For your safety

Conformity to current standards ®

Safety instructions in this manual

SORVALL centrifuges are manufactured and tested according to the following standards and regulations:

This symbol denotes potential hazards to persons.

- For all voltages

This symbol denotes potential damage to the centrifuge or parts in its immediate surroundings.

• IEC 61010 - For 120 V only •

General comments are marked with this symbol. In addition, you are asked to adhere to the pertinent regulations, in Germany

- For 230 V only •

• Regulations for prevention of accidents BGV A2 • Regulations for prevention of accidents VBG 5 • Regulations for prevention of accidents VBG 7z - with cooled devices additionally

Please get acquainted with the details of the test standards from the technical data.

• Regulations for prevention of accidents BGV D4

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For your safety Notes

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Description of the Legend™ MACH 1.6

The Legend™ MACH 1.6 / R

Description

The figure below shows a Legend™ MACH 1.6 R with an opened lid and a swinging bucket rotor installed.

The Legend™ MACH 1.6 / R is a general-purpose tabletop centrifuge for biotechnological and pharmaceutical research that moves high capacity centrifugation onto the fast track. It spins more tubes at higher RCFs more rapidly than competitive instruments and can process nominally 1.6 liters of sample in a single run. There are various rotors with high RCF values that can accommodate a wide range of accessories for all common tube types, micro titer plates and deep-wells. The user-friendly "QUIKset®" control panel permits easy selection of speed, RCF value, run time and deceleration profile, as well as temperature of the Legend™ MACH 1.6 R. You can switch from speed to RCF display and vice versa, with a touch of a button and even during a run.

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Description of the Legend™ MACH 1.6

Safety systems The Legend™ MACH 1.6 / R is equipped with a number of safety systems: • Housing and lid are constructed of 6 mm steel. • Lid with window • Lid lock with safety check You can only open the centrifuge lid when the power is turned on and the rotor has come to a stop. You can only start the centrifuge if the lid is properly locked.

Parts supplied Accessories supplied with the centrifuge are: − power cord − a special wrench for securing the rotor (As seen in the picture) − condensed operating instructions

• Automatic rotor identification • Electronic unbalance detection as a function of rotor (SMARTspinTM)

Do not tamper with the safety systems!

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− corrosion protective oil The printed documents consist of the delivery notes and this Manual.

Description of the Legend™ MACH 1.6

Function and features Basic unit/ function

Description / feature

Cabinet / frame

Galvanized steel

Chamber

Stainless steel

Drive

Brushless induction drive

Key pad and display

Key pad and display elements covered by an easy-care continues surface

Control

Microprocessor driven by "QUIKset®"

Main memory

Recalls last run parameters

Advanced features

RCF-programming, quick run, temperature control during standby

Deceleration profiles

3 deceleration profiles

Rotor identification

Automatic

TM

SMARTspin unbalance detection system

Electronic, effective as a function of rotor and speed

Soft touch lid lock

Motor assisted lid locking

(* only with refrigeration unit)

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Description of the Legend™ MACH 1.6

The "QUIKset®" user interface Function

Feature

Deceleration profile

1 = slowest, ... 3 = fastest deceleration curve

Setting speed by rpm

Adjustable from 300 rpm to 15 000 rpm, in 10 rpm increments

RCF selection

Upon actuation of RCF switch , the RCF value can then be entered

Time selection

Adjustable in minutes from 1 min to 9 h 59 min, or extended time mode from 1 min to 99 h, ”hld"-mode: continuous operation

Run time display in "quick run" mode

Between 1 s and 60 s in seconds´ steps, above in minutes´ steps

Setting temperature *

Adjustable from -9°C to +40°C, in one degree increments

End of centrifugation

The speed display will read "end"

(* only with refrigeration unit)

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Description of the Legend™ MACH 1.6

Function

Feature

Lid opening

Automatic unlocking via ”open lid" key ( ) (unlocking in case of power failure: see chapter ”Troubleshooting")

Start

Start key (

)

Stop

Stop key (

)

"Quick Run" mode

Pressing the "quick run" key ( ) activates maximum acceleration up to the maximum permissible speed of rotor; upon key release the centrifuge stops with maximum deceleration power.

Diagnostic messages

•

Alternating display ”Rotor"/maximum speed or RCF (speed selected exceeds max. speed of the rotor)

•

Lid has not been lifted off the lock during opening: display ”lift lid" (manual lifting of lid is required)

•

General instrument malfunction (error messages with ERROR codes, see "Troubleshooting")

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Description of the Legend™ MACH 1.6

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Before use

Before use Centrifuge transport and installation After opening the box remove the protective materials. When transporting the centrifuge, consider its weight (see "Technical Data"); always grab it on both sides ensuring that enough helpers are around. Do not lift on the front panel!

The centrifuge can be damaged by jolting during the transport! Transport the centrifuge only in the upright position using proper containment and secure it properly. Handle the centrifuge carefully.

Proper location The centrifuge may only be used indoors. Its location must meet the following criteria: • A safety zone of at least 30 cm (12 inches) around the centrifuge must be maintained. Hazardous materials must not be stored beside the centrifuge during its use. • The laboratory bench or centrifuge trolley must be stable and resonance-free. A good support is provided by a laboratory bench or a centrifuge cart with lockable casters. • To ensure sufficient air circulation, a minimum distance from the wall of 10 cm (4 inches) at the back and of 15 cm (6 inches) on each side must be kept.

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Before use • The centrifuge must be protected from heat and direct sunlight. • The location must be well ventilated at all times. UV rays reduce the durability of plastics. Protect the centrifuge, rotors and plastic accessories from direct sunlight.

Main connection Connect the centrifuge only to a grounded main power supply. Make sure that the power cord is compatible with the valid safety regulations and that your main voltage and frequency correspond to the specifications on the instrument label. Make sure that the centrifuge is switched off (on the right-hand side of front panel) before connecting electrical wire to main power supply.

RS232 interface specifications Main connection

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Rotors and accessories

Rotors and accessories A rotor is not included as part of a Legend™ centrifuge. A large variety of rotors are available as accessories. In addition, there are adapters and reduction sleeves for a variety of commercially available tubes and bottles. For a complete list of available tubes, bottles, adapters and accessories, please refer to our current sales documentation. For more information you can visit our web site at http://www.Kendro.com

Legend™ MACH 1.6

(air cooled)

During centrifugation, heat is generated by air friction between the rapidly spinning rotor and the air inside the rotor chamber. The continuous air flow in the air cooled centrifuge is restricting the temperature rise of the samples. The equilibrium temperature rise depends on the ambient temperature, the type of rotor, the speed, the duration and the number of runs. Use caution when touching rotors as they may be hot after long runs at high speed.

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Rotors and accessories

Rotors for the Legend™ MACH 1.6 Table 1: Rotors for Legend™ MACH 1.6 120V

(Differences of 230V instruments are shown in parentheses)

Rotor designation

Swinging Bucket Rotor TTH 400 75002000 Rectangular bucket 150 ml 75002001

With bucket Order no.

Round bucket 400 ml 75002002

Maximum permissible load [ g ]

4 x 540

Maximum speed nmax [ rpm ]

4500

( 4700 )

4700

Maximum RCF value at nmax

4234

( 4618 )

4618

Radius (max.)

18.7

[ cm ]

Acceleration / deceleration time [ s ]

4 x 570

18.7

50 / 40

( 40 / 35 )

55 / 40

relative to room temperature 23°C, run time 1 hour

14.0

( 12.0 )

13.0

Aerosol-tight *

Yes (with cap 75002003)

Yes (with cap 75002004)

Autoclavable

121°C

121°C

Heating of samples at nmax [ °C ]

* Tested and approved by CAMR, Porton-Down, UK

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( 40 / 35 )